id author title date pages extension mime words sentences flesch summary cache txt cord-337701-56tmg38b Xiao, Yan Comparison of three TaqMan Real-Time Reverse Transcription-PCR assays in detecting SARS-CoV-2 2020-07-06 .txt text/plain 2122 120 57 In the present study, we evaluated the sensitivity, specificity, amplification efficiency, and linear detection ranges of three qRT-PCR assays, including the assays developed by our group (IPBCAMS), and the assays recommended by WHO and China CDC (CCDC). No effect of the 152 co-existed other viral nucleic acids on the LOD and the linear detection range was 153 observed, although higher Ct values were generated than those of RNA transcript 154 alone as template in all of the three qRT-PCR assays. Although most of the evaluated 232 assays exhibited good sensitivity, specificity, reproducibility and wide linear detection 233 range, performance test with clinical specimens from suspected COVID-19 patients 234 suggested that the N gene assay in IPBCAMS assays and CCDC assays, and the ORF 235 1b gene assays in IPBCAMS assays were the preferred qRT-PCR assays for accurate 236 detection of SARS-CoV-2. ./cache/cord-337701-56tmg38b.txt ./txt/cord-337701-56tmg38b.txt