id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-342657-od48cntc	Klemm, Theresa	Mechanism and inhibition of SARS-CoV-2 PLpro	2020-06-19		.txt	text/plain	1704	110	65	More variability is seen in MERS PLpro, which binds to ubiquitin and 138 ISG15 CTD similarly through its ability to 'close' the Fingers subdomain 26 (see 139 discussion in Extended Data Fig. 4) . A 1536-well low-volume high-throughput assay previously used to identify inhibitors Together, our data suggests that a repurposing strategy using 3727 unique known 199 drugs towards SARS2 PLpro is unlikely to yield drug candidates, and highlights the 200 importance of a counterscreen in assessing the validity of hits coming from a screen 201 of known drugs before any conclusions on their therapeutic potential can be drawn. Since it had previously 237 been shown that these inhibitors are specific for PLpro over human DUBs 1 (also see 238 Figure 3b ) and since treatment with rac5c did not affect Lys48-linked polyubiquitin in 239 the absence of nsp3 expression (Extended Data Fig 8f) , the effect of rac5c on 240 Lys48-polyubiquitin is likely due to inhibition of nsp3/PLpro.	./cache/cord-342657-od48cntc.txt	./txt/cord-342657-od48cntc.txt