id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-354725-lqio7l8k	Arumugam, Arunkumar	A Rapid COVID-19 RT-PCR Detection Assay for Low Resource Settings	2020-04-30		.txt	text/plain	3478	203	64	Using COVID-19 positive clinical specimens, we demonstrated that RT-PCR assays can be performed in as little as 12 minutes using untreated samples, heat-inactivated samples, or extracted RNA templates. 5 Despite its established performance in sensitivity and specificity, RT-qPCR requires expensive equipment such as RNA isolation instruments and real-time PCR thermal cyclers, which are not available in many resource limiting settings. Two water baths (one for denaturation and one for the reverse transcription and then the annealing/extension steps) were made using food storage plastic containers heated by sous vide immersion heaters. We tested this rapid water bath RT-PCR approach using untreated or heat-inactivated samples directly added to one-step RT-PCR master mixes without an RNA extraction step. Extracted templates from COVID-19 positive clinical specimens and contrived negative samples were tested using water bath-based RT-PCR. With a 3-minute RNA extraction protocol, we were able to get positive RT-PCR results with all ten COVID-19 positive clinical samples.	./cache/cord-354725-lqio7l8k.txt	./txt/cord-354725-lqio7l8k.txt