id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-332572-9h26mj7w	Wang, Jinfeng	Development and validation of the isothermal recombinase polymerase amplification assays for rapid detection of Mycoplasma ovipneumoniae in sheep	2020-06-01		.txt	text/plain	2909	144	47	title: Development and validation of the isothermal recombinase polymerase amplification assays for rapid detection of Mycoplasma ovipneumoniae in sheep ovipneumoniae DNA was detected in 29 samples in the real-time RPA, 31 samples in the LFS RPA and 32 samples in the real-time PCR assay. In this study, a real-time RPA assay using the exo probe and a LFS RPA assay using the nfo probe combined with lateral flow strip were developed for rapid, specific and sensitive detection of M. ovipneumoniae DNA was detected in 29 (26.12%), 31 (27.93%) and 32 (28.83%) samples by the real-time RPA, LFS RPA and real-time PCR, respectively (Table 1 ). The real-time RPA and LFS RPA assays demonstrated the comparable performance in detecting the 111 sheep clinical samples. The developed real-time RPA and LFS RPA assays are highly specific and sensitive for detection of M.	./cache/cord-332572-9h26mj7w.txt	./txt/cord-332572-9h26mj7w.txt