id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-008613-tysyq6o4	Thomas, Sheila M.	Two mRNAs that differ by two nontemplated nucleotides encode the amino coterminal proteins P and V of the paramyxovirus SV5	1988-09-09		.txt	text/plain	8410	433	60	Simian virus 5 (SV5), a prototype paramyxovirus, has a single-stranded, negative sense genomic RNA (vRNA) approximately 15,000 nucleotides in chain length that is transcribed in infected cells by the virion-associated RNA transcriptase to yield virus-specific mRNAs. The SV5 "P" gene has been shown to encode both the P protein (Mr = 44,000), and protein V (Mr = 24,000) by the arrest of translation in vitro of both P and V using a cDNA clone derived from SV5-specific mRNAs (Paterson et al., 1984) . Antigenic Region Mapping of the P and V Monoclonal Antibodies on the P and V Gene Using T7 RNA Polymerase Runoff Transcripts, In Vitro Translation, and Immunoprecipitation The full-length P203-1 DNA insert cloned using Xbal linkers (XX) and a Hindlll to Xbal fragment (HX) containing the 3' two-thirds of the P203-1 DNA were placed under the control of the T7 promoter in the plasmid pGEM-2.	./cache/cord-008613-tysyq6o4.txt	./txt/cord-008613-tysyq6o4.txt