id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-255856-0xqllbzz	Florman, Harvey M.	O-linked oligosaccharides of mouse egg ZP3 account for its sperm receptor activity	1985-05-31		.txt	text/plain	8973	445	50	A specific size class of O-linked oligosaccharides, recovered following mild alkaline hydrolysis and reduction of ZP3, is shown to possess sperm receptor activity and to bind to sperm. In these experiments, a competition assay (Bleil and Wassarman, 1980a; Florman et al., 1984) was used to determine the ability of oligosaccharides, derived from either solubilized zonae pellucidae or purified zona pellucida glycoproteins, to inhibit the binding of sperm to eggs in vitro ("sperm receptor activity"). On the other hand, egg zonae pellucidae subjected to mild alkaline hydrolysis, under the conditions described above, lost approximately 90% of the sperm receptor activity present in untreated samples and in samples treated only with distilled water (Figure 2) . Initial experiments demonstrated that radiolabeled (3H-NaBH,) oligosaccharides, possessing sperm receptor activity, could be recovered from alkaline hydrolysates of egg zonae pellucidae.	./cache/cord-255856-0xqllbzz.txt	./txt/cord-255856-0xqllbzz.txt