id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-259681-k9cnikqk	Johnson, David C.	O-linked oligosaccharides are acquired by herpes simplex virus glycoproteins in the Golgi apparatus	1983-03-31		.txt	text/plain	5745	267	45	These studies were carried out not only under conditions designed to allow normal glycoprotein processing, but also in the presence of monensin, an ionophore known to cause accumulation of secreted and membrane proteins and virions in Golgi-derived vacuoles (Tartakoff and Vassalli, 1978; Uchida et al., 1979; Johnson and Schlesinger, 1980; Johnson and Spear, 1982) , and in ricin-resistant cells defective in the processing of N-linked oligosaccharides. Our results indicate that extension of O-linked oligosaccharides to yield chains of sufficient number or size to affect electrophoretic mobilities of the glycoproteins, and probably also addition of the first amino acid-linked GalNAc residues, occur in the Golgi apparatus subsequent to addition of fatty acid and coincident with the processing of high-mannose-type Nlinked oligosaccharides to complex-type oligosaccharides. The "C-labeled HSVl glycoproteins gC and gD were isolated on preparative SDS-polyacrylamide gels and treated with 0.05 M NaOH, 1 M NaBHa at 45°C for 14-20 hr (mild alkaline borohydride), conditions shown to selectively release O-linked oligosaccharides (Spiro, 1966; Marshall and Neuberger, 1977) .	./cache/cord-259681-k9cnikqk.txt	./txt/cord-259681-k9cnikqk.txt