id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-307021-uppekume	Crooke, Elliott	ProOmpA is stabilized for membrane translocation by either purified E. coli trigger factor or canine signal recognition particle	1988-09-23		.txt	text/plain	5574	347	62	Pro-OmpA preincubated at 21°C with membrane vesicles lost competence for subsequent membrane translocation ( Figure 6 , lane 11) while preincubation in the presence of membranes and trigger factor, but without ATP and NADH (lane 12), stabilized the assembly-active precursor. SRP Stabilizes ProOmpA for Membrane Translocation Like trigger factor, canine SRP ha8 been shown to recognize presecretory proteins, although until now its activity has been assayed in the context of a polysome. Nikko1 does not inhibit the membrane assembly of proOmpA that has already formed a complex with trigger factor (Figure 9 , lane 3, no detergent; lane 4, detergent added), and thus has no effect on the membrane translocation "machinery" per se or on the protease protection assay. It is likely that other soluble proteins, such as those encoded by the secA and se& genes, would bind to our preparations of plasma membrane vesicles or, if added to the in vitro translocation in the absence (lane 2) or presence (lanes 3 and 4) of trigger factor (100 ng).	./cache/cord-307021-uppekume.txt	./txt/cord-307021-uppekume.txt