id	sid	tid	token	lemma	pos
cord-279463-bli8hwda	1	1	key	key	JJ
cord-279463-bli8hwda	1	2	:	:	:
cord-279463-bli8hwda	1	3	cord-279463-bli8hwda	cord-279463-bli8hwda	JJ
cord-279463-bli8hwda	1	4	authors	author	NNS
cord-279463-bli8hwda	1	5	:	:	:
cord-279463-bli8hwda	2	1	Lipp	Lipp	NNP
cord-279463-bli8hwda	2	2	,	,	,
cord-279463-bli8hwda	2	3	Joachim	Joachim	NNP
cord-279463-bli8hwda	2	4	;	;	:
cord-279463-bli8hwda	2	5	Dobberstein	Dobberstein	NNP
cord-279463-bli8hwda	2	6	,	,	,
cord-279463-bli8hwda	2	7	Bernhard	Bernhard	NNP
cord-279463-bli8hwda	2	8	title	title	NN
cord-279463-bli8hwda	2	9	:	:	:
cord-279463-bli8hwda	2	10	The	the	DT
cord-279463-bli8hwda	2	11	membrane	membrane	NN
cord-279463-bli8hwda	2	12	-	-	HYPH
cord-279463-bli8hwda	2	13	spanning	span	VBG
cord-279463-bli8hwda	2	14	segment	segment	NN
cord-279463-bli8hwda	2	15	of	of	IN
cord-279463-bli8hwda	2	16	invariant	invariant	JJ
cord-279463-bli8hwda	2	17	chain	chain	NN
cord-279463-bli8hwda	2	18	(	(	-LRB-
cord-279463-bli8hwda	2	19	Iγ	Iγ	NNP
cord-279463-bli8hwda	2	20	)	)	-RRB-
cord-279463-bli8hwda	2	21	contains	contain	VBZ
cord-279463-bli8hwda	2	22	a	a	DT
cord-279463-bli8hwda	2	23	potentially	potentially	RB
cord-279463-bli8hwda	2	24	cleavable	cleavable	JJ
cord-279463-bli8hwda	2	25	signal	signal	NN
cord-279463-bli8hwda	2	26	sequence	sequence	NN
cord-279463-bli8hwda	2	27	date	date	NN
cord-279463-bli8hwda	2	28	:	:	:
cord-279463-bli8hwda	2	29	1986	1986	CD
cord-279463-bli8hwda	2	30	-	-	SYM
cord-279463-bli8hwda	2	31	09	09	CD
cord-279463-bli8hwda	2	32	-	-	SYM
cord-279463-bli8hwda	2	33	26	26	CD
cord-279463-bli8hwda	2	34	journal	journal	NN
cord-279463-bli8hwda	2	35	:	:	:
cord-279463-bli8hwda	2	36	Cell	cell	NN
cord-279463-bli8hwda	2	37	DOI	doi	NN
cord-279463-bli8hwda	2	38	:	:	:
cord-279463-bli8hwda	2	39	10.1016/0092	10.1016/0092	CD
cord-279463-bli8hwda	2	40	-	-	HYPH
cord-279463-bli8hwda	2	41	8674(86)90710	8674(86)90710	CD
cord-279463-bli8hwda	2	42	-	-	HYPH
cord-279463-bli8hwda	2	43	5	5	CD
cord-279463-bli8hwda	2	44	sha	sha	NNP
cord-279463-bli8hwda	2	45	:	:	:
cord-279463-bli8hwda	2	46	9b370f9b62666750df6efbabe7efa6811ee8c338	9b370f9b62666750df6efbabe7efa6811ee8c338	NNP
cord-279463-bli8hwda	2	47	doc_id	doc_id	NNPS
cord-279463-bli8hwda	2	48	:	:	:
cord-279463-bli8hwda	2	49	279463	279463	CD
cord-279463-bli8hwda	2	50	cord_uid	cord_uid	NNS
cord-279463-bli8hwda	2	51	:	:	:
cord-279463-bli8hwda	3	1	bli8hwda	bli8hwda	NNP
cord-279463-bli8hwda	3	2	Abstract	abstract	JJ
cord-279463-bli8hwda	4	1	The	the	DT
cord-279463-bli8hwda	4	2	human	human	JJ
cord-279463-bli8hwda	4	3	invariant	invariant	JJ
cord-279463-bli8hwda	4	4	chain	chain	NN
cord-279463-bli8hwda	4	5	(	(	-LRB-
cord-279463-bli8hwda	4	6	Iγ	Iγ	NNP
cord-279463-bli8hwda	4	7	)	)	-RRB-
cord-279463-bli8hwda	4	8	of	of	IN
cord-279463-bli8hwda	4	9	class	class	NN
cord-279463-bli8hwda	4	10	II	II	NNP
cord-279463-bli8hwda	4	11	histocompatibility	histocompatibility	NN
cord-279463-bli8hwda	4	12	antigens	antigen	NNS
cord-279463-bli8hwda	4	13	spans	span	VBZ
cord-279463-bli8hwda	4	14	the	the	DT
cord-279463-bli8hwda	4	15	membrane	membrane	NN
cord-279463-bli8hwda	4	16	of	of	IN
cord-279463-bli8hwda	4	17	the	the	DT
cord-279463-bli8hwda	4	18	endoplasmic	endoplasmic	NN
cord-279463-bli8hwda	4	19	reticulum	reticulum	NN
cord-279463-bli8hwda	4	20	once	once	RB
cord-279463-bli8hwda	4	21	.	.	.
cord-279463-bli8hwda	5	1	It	-PRON-	PRP
cord-279463-bli8hwda	5	2	exposes	expose	VBZ
cord-279463-bli8hwda	5	3	a	a	DT
cord-279463-bli8hwda	5	4	small	small	JJ
cord-279463-bli8hwda	5	5	amino	amino	NN
cord-279463-bli8hwda	5	6	-	-	HYPH
cord-279463-bli8hwda	5	7	terminal	terminal	NN
cord-279463-bli8hwda	5	8	domain	domain	NN
cord-279463-bli8hwda	5	9	on	on	IN
cord-279463-bli8hwda	5	10	the	the	DT
cord-279463-bli8hwda	5	11	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	5	12	side	side	NN
cord-279463-bli8hwda	5	13	and	and	CC
cord-279463-bli8hwda	5	14	a	a	DT
cord-279463-bli8hwda	5	15	carboxyterminal	carboxyterminal	JJ
cord-279463-bli8hwda	5	16	,	,	,
cord-279463-bli8hwda	5	17	glycosylated	glycosylated	JJ
cord-279463-bli8hwda	5	18	domain	domain	NN
cord-279463-bli8hwda	5	19	on	on	IN
cord-279463-bli8hwda	5	20	the	the	DT
cord-279463-bli8hwda	5	21	exoplasmic	exoplasmic	JJ
cord-279463-bli8hwda	5	22	side	side	NN
cord-279463-bli8hwda	5	23	of	of	IN
cord-279463-bli8hwda	5	24	the	the	DT
cord-279463-bli8hwda	5	25	membrane	membrane	NN
cord-279463-bli8hwda	5	26	.	.	.
cord-279463-bli8hwda	6	1	When	when	WRB
cord-279463-bli8hwda	6	2	the	the	DT
cord-279463-bli8hwda	6	3	exoplasmic	exoplasmic	JJ
cord-279463-bli8hwda	6	4	domain	domain	NN
cord-279463-bli8hwda	6	5	of	of	IN
cord-279463-bli8hwda	6	6	Iγ	Iγ	NNP
cord-279463-bli8hwda	6	7	is	be	VBZ
cord-279463-bli8hwda	6	8	replaced	replace	VBN
cord-279463-bli8hwda	6	9	by	by	IN
cord-279463-bli8hwda	6	10	the	the	DT
cord-279463-bli8hwda	6	11	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	6	12	protein	protein	NN
cord-279463-bli8hwda	6	13	chloramphenicol	chloramphenicol	NNP
cord-279463-bli8hwda	6	14	acetyltransferase	acetyltransferase	NNP
cord-279463-bli8hwda	6	15	(	(	-LRB-
cord-279463-bli8hwda	6	16	CAT	CAT	NNP
cord-279463-bli8hwda	6	17	)	)	-RRB-
cord-279463-bli8hwda	6	18	,	,	,
cord-279463-bli8hwda	6	19	CAT	CAT	NNP
cord-279463-bli8hwda	6	20	becomes	become	VBZ
cord-279463-bli8hwda	6	21	the	the	DT
cord-279463-bli8hwda	6	22	exoplasmic	exoplasmic	JJ
cord-279463-bli8hwda	7	1	,	,	,
cord-279463-bli8hwda	7	2	glycosylated	glycosylated	JJ
cord-279463-bli8hwda	7	3	domain	domain	NN
cord-279463-bli8hwda	7	4	of	of	IN
cord-279463-bli8hwda	7	5	the	the	DT
cord-279463-bli8hwda	7	6	resulting	result	VBG
cord-279463-bli8hwda	7	7	membrane	membrane	NN
cord-279463-bli8hwda	7	8	protein	protein	NN
cord-279463-bli8hwda	7	9	IγCAT∗.	IγCAT∗.	NNP
cord-279463-bli8hwda	7	10	Deletion	Deletion	NNP
cord-279463-bli8hwda	7	11	of	of	IN
cord-279463-bli8hwda	7	12	the	the	DT
cord-279463-bli8hwda	7	13	hydrophilic	hydrophilic	JJ
cord-279463-bli8hwda	7	14	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	7	15	domain	domain	NN
cord-279463-bli8hwda	7	16	from	from	IN
cord-279463-bli8hwda	7	17	IγCAT	IγCAT	NNP
cord-279463-bli8hwda	7	18	gives	give	VBZ
cord-279463-bli8hwda	7	19	rise	rise	NN
cord-279463-bli8hwda	7	20	to	to	IN
cord-279463-bli8hwda	7	21	a	a	DT
cord-279463-bli8hwda	7	22	secreted	secrete	VBN
cord-279463-bli8hwda	7	23	protein	protein	NN
cord-279463-bli8hwda	7	24	from	from	IN
cord-279463-bli8hwda	7	25	which	which	WDT
cord-279463-bli8hwda	7	26	an	an	DT
cord-279463-bli8hwda	7	27	amino	amino	NN
cord-279463-bli8hwda	7	28	-	-	HYPH
cord-279463-bli8hwda	7	29	terminal	terminal	NN
cord-279463-bli8hwda	7	30	segment	segment	NN
cord-279463-bli8hwda	7	31	is	be	VBZ
cord-279463-bli8hwda	7	32	cleaved	cleave	VBN
cord-279463-bli8hwda	7	33	,	,	,
cord-279463-bli8hwda	7	34	most	most	RBS
cord-279463-bli8hwda	7	35	likely	likely	JJ
cord-279463-bli8hwda	7	36	by	by	IN
cord-279463-bli8hwda	7	37	signal	signal	NN
cord-279463-bli8hwda	7	38	peptidase	peptidase	NN
cord-279463-bli8hwda	7	39	.	.	.
cord-279463-bli8hwda	8	1	We	-PRON-	PRP
cord-279463-bli8hwda	8	2	conclude	conclude	VBP
cord-279463-bli8hwda	8	3	that	that	IN
cord-279463-bli8hwda	8	4	the	the	DT
cord-279463-bli8hwda	8	5	membrane	membrane	NN
cord-279463-bli8hwda	8	6	-	-	HYPH
cord-279463-bli8hwda	8	7	spanning	span	VBG
cord-279463-bli8hwda	8	8	region	region	NN
cord-279463-bli8hwda	8	9	of	of	IN
cord-279463-bli8hwda	8	10	Iγ	Iγ	NNP
cord-279463-bli8hwda	8	11	contains	contain	VBZ
cord-279463-bli8hwda	8	12	a	a	DT
cord-279463-bli8hwda	8	13	signal	signal	JJ
cord-279463-bli8hwda	8	14	sequence	sequence	NN
cord-279463-bli8hwda	8	15	in	in	IN
cord-279463-bli8hwda	8	16	its	-PRON-	PRP$
cord-279463-bli8hwda	8	17	amino	amino	NN
cord-279463-bli8hwda	8	18	-	-	HYPH
cord-279463-bli8hwda	8	19	terminal	terminal	NN
cord-279463-bli8hwda	8	20	half	half	NN
cord-279463-bli8hwda	8	21	and	and	CC
cord-279463-bli8hwda	8	22	that	that	IN
cord-279463-bli8hwda	8	23	hydrophilic	hydrophilic	JJ
cord-279463-bli8hwda	8	24	residues	residue	NNS
cord-279463-bli8hwda	8	25	at	at	IN
cord-279463-bli8hwda	8	26	the	the	DT
cord-279463-bli8hwda	8	27	amino	amino	NN
cord-279463-bli8hwda	8	28	-	-	HYPH
cord-279463-bli8hwda	8	29	terminal	terminal	NN
cord-279463-bli8hwda	8	30	end	end	NN
cord-279463-bli8hwda	8	31	of	of	IN
cord-279463-bli8hwda	8	32	a	a	DT
cord-279463-bli8hwda	8	33	signal	signal	JJ
cord-279463-bli8hwda	8	34	sequence	sequence	NN
cord-279463-bli8hwda	8	35	can	can	MD
cord-279463-bli8hwda	8	36	determine	determine	VB
cord-279463-bli8hwda	8	37	cleavage	cleavage	NN
cord-279463-bli8hwda	8	38	by	by	IN
cord-279463-bli8hwda	8	39	signal	signal	NN
cord-279463-bli8hwda	8	40	peptidase	peptidase	NN
cord-279463-bli8hwda	8	41	.	.	.
cord-279463-bli8hwda	9	1	The	the	DT
cord-279463-bli8hwda	9	2	human	human	JJ
cord-279463-bli8hwda	9	3	invariant	invariant	JJ
cord-279463-bli8hwda	9	4	chain	chain	NN
cord-279463-bli8hwda	9	5	(	(	-LRB-
cord-279463-bli8hwda	9	6	ly	ly	NNP
cord-279463-bli8hwda	9	7	)	)	-RRB-
cord-279463-bli8hwda	9	8	of	of	IN
cord-279463-bli8hwda	9	9	class	class	NN
cord-279463-bli8hwda	9	10	II	II	NNP
cord-279463-bli8hwda	9	11	histocompatibility	histocompatibility	NN
cord-279463-bli8hwda	9	12	antigens	antigen	NNS
cord-279463-bli8hwda	9	13	spans	span	VBZ
cord-279463-bli8hwda	9	14	the	the	DT
cord-279463-bli8hwda	9	15	membrane	membrane	NN
cord-279463-bli8hwda	9	16	of	of	IN
cord-279463-bli8hwda	9	17	the	the	DT
cord-279463-bli8hwda	9	18	endoplasmic	endoplasmic	NN
cord-279463-bli8hwda	9	19	reticulum	reticulum	NN
cord-279463-bli8hwda	9	20	once	once	RB
cord-279463-bli8hwda	9	21	.	.	.
cord-279463-bli8hwda	10	1	It	-PRON-	PRP
cord-279463-bli8hwda	10	2	exposes	expose	VBZ
cord-279463-bli8hwda	10	3	a	a	DT
cord-279463-bli8hwda	10	4	small	small	JJ
cord-279463-bli8hwda	10	5	amino	amino	NN
cord-279463-bli8hwda	10	6	-	-	HYPH
cord-279463-bli8hwda	10	7	terminal	terminal	NN
cord-279463-bli8hwda	10	8	domain	domain	NN
cord-279463-bli8hwda	10	9	on	on	IN
cord-279463-bli8hwda	10	10	the	the	DT
cord-279463-bli8hwda	10	11	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	10	12	side	side	NN
cord-279463-bli8hwda	10	13	and	and	CC
cord-279463-bli8hwda	10	14	a	a	DT
cord-279463-bli8hwda	10	15	carboxyterminal	carboxyterminal	JJ
cord-279463-bli8hwda	10	16	,	,	,
cord-279463-bli8hwda	10	17	glycosylated	glycosylated	JJ
cord-279463-bli8hwda	10	18	domain	domain	NN
cord-279463-bli8hwda	10	19	on	on	IN
cord-279463-bli8hwda	10	20	the	the	DT
cord-279463-bli8hwda	10	21	exoplasmic	exoplasmic	JJ
cord-279463-bli8hwda	10	22	side	side	NN
cord-279463-bli8hwda	10	23	of	of	IN
cord-279463-bli8hwda	10	24	the	the	DT
cord-279463-bli8hwda	10	25	membrane	membrane	NN
cord-279463-bli8hwda	10	26	.	.	.
cord-279463-bli8hwda	11	1	When	when	WRB
cord-279463-bli8hwda	11	2	the	the	DT
cord-279463-bli8hwda	11	3	exoplasmic	exoplasmic	JJ
cord-279463-bli8hwda	11	4	domain	domain	NN
cord-279463-bli8hwda	11	5	of	of	IN
cord-279463-bli8hwda	11	6	ly	ly	NNP
cord-279463-bli8hwda	11	7	is	be	VBZ
cord-279463-bli8hwda	11	8	replaced	replace	VBN
cord-279463-bli8hwda	11	9	by	by	IN
cord-279463-bli8hwda	11	10	the	the	DT
cord-279463-bli8hwda	11	11	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	11	12	protein	protein	NN
cord-279463-bli8hwda	11	13	chloramphenicol	chloramphenicol	NNP
cord-279463-bli8hwda	11	14	acetyltransferase	acetyltransferase	NNP
cord-279463-bli8hwda	11	15	(	(	-LRB-
cord-279463-bli8hwda	11	16	CAT	CAT	NNP
cord-279463-bli8hwda	11	17	)	)	-RRB-
cord-279463-bli8hwda	11	18	,	,	,
cord-279463-bli8hwda	11	19	CAT	CAT	NNP
cord-279463-bli8hwda	11	20	becomes	become	VBZ
cord-279463-bli8hwda	11	21	the	the	DT
cord-279463-bli8hwda	11	22	exoplasmic	exoplasmic	JJ
cord-279463-bli8hwda	11	23	,	,	,
cord-279463-bli8hwda	11	24	glycosylated	glycosylated	JJ
cord-279463-bli8hwda	11	25	domain	domain	NN
cord-279463-bli8hwda	11	26	of	of	IN
cord-279463-bli8hwda	11	27	the	the	DT
cord-279463-bli8hwda	11	28	resulting	result	VBG
cord-279463-bli8hwda	11	29	membrane	membrane	NN
cord-279463-bli8hwda	11	30	protein	protein	NN
cord-279463-bli8hwda	11	31	I$AT	I$AT	NNS
cord-279463-bli8hwda	12	1	*	*	NFP
cord-279463-bli8hwda	12	2	.	.	.
cord-279463-bli8hwda	13	1	Deletion	deletion	NN
cord-279463-bli8hwda	13	2	of	of	IN
cord-279463-bli8hwda	13	3	the	the	DT
cord-279463-bli8hwda	13	4	hydrophilic	hydrophilic	JJ
cord-279463-bli8hwda	13	5	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	13	6	domain	domain	NN
cord-279463-bli8hwda	13	7	from	from	IN
cord-279463-bli8hwda	13	8	l$XT	l$XT	NNP
cord-279463-bli8hwda	13	9	gives	give	VBZ
cord-279463-bli8hwda	13	10	rise	rise	NN
cord-279463-bli8hwda	13	11	to	to	IN
cord-279463-bli8hwda	13	12	a	a	DT
cord-279463-bli8hwda	13	13	secreted	secrete	VBN
cord-279463-bli8hwda	13	14	protein	protein	NN
cord-279463-bli8hwda	13	15	from	from	IN
cord-279463-bli8hwda	13	16	which	which	WDT
cord-279463-bli8hwda	13	17	an	an	DT
cord-279463-bli8hwda	13	18	amino	amino	NN
cord-279463-bli8hwda	13	19	-	-	HYPH
cord-279463-bli8hwda	13	20	terminal	terminal	NN
cord-279463-bli8hwda	13	21	segment	segment	NN
cord-279463-bli8hwda	13	22	is	be	VBZ
cord-279463-bli8hwda	13	23	cleaved	cleave	VBN
cord-279463-bli8hwda	13	24	,	,	,
cord-279463-bli8hwda	13	25	most	most	RBS
cord-279463-bli8hwda	13	26	likely	likely	JJ
cord-279463-bli8hwda	13	27	by	by	IN
cord-279463-bli8hwda	13	28	signal	signal	NN
cord-279463-bli8hwda	13	29	peptidase	peptidase	NN
cord-279463-bli8hwda	13	30	.	.	.
cord-279463-bli8hwda	14	1	We	-PRON-	PRP
cord-279463-bli8hwda	14	2	conclude	conclude	VBP
cord-279463-bli8hwda	14	3	that	that	IN
cord-279463-bli8hwda	14	4	the	the	DT
cord-279463-bli8hwda	14	5	membranespanning	membranespanne	VBG
cord-279463-bli8hwda	14	6	region	region	NN
cord-279463-bli8hwda	14	7	of	of	IN
cord-279463-bli8hwda	14	8	ly	ly	NNP
cord-279463-bli8hwda	14	9	contains	contain	VBZ
cord-279463-bli8hwda	14	10	a	a	DT
cord-279463-bli8hwda	14	11	signal	signal	JJ
cord-279463-bli8hwda	14	12	sequence	sequence	NN
cord-279463-bli8hwda	14	13	in	in	IN
cord-279463-bli8hwda	14	14	its	-PRON-	PRP$
cord-279463-bli8hwda	14	15	amino	amino	NN
cord-279463-bli8hwda	14	16	-	-	HYPH
cord-279463-bli8hwda	14	17	terminal	terminal	NN
cord-279463-bli8hwda	14	18	half	half	NN
cord-279463-bli8hwda	14	19	and	and	CC
cord-279463-bli8hwda	14	20	that	that	IN
cord-279463-bli8hwda	14	21	hydrophilic	hydrophilic	JJ
cord-279463-bli8hwda	14	22	residues	residue	NNS
cord-279463-bli8hwda	14	23	at	at	IN
cord-279463-bli8hwda	14	24	the	the	DT
cord-279463-bli8hwda	14	25	amino	amino	NN
cord-279463-bli8hwda	14	26	-	-	HYPH
cord-279463-bli8hwda	14	27	terminal	terminal	NN
cord-279463-bli8hwda	14	28	end	end	NN
cord-279463-bli8hwda	14	29	of	of	IN
cord-279463-bli8hwda	14	30	a	a	DT
cord-279463-bli8hwda	14	31	signal	signal	JJ
cord-279463-bli8hwda	14	32	sequence	sequence	NN
cord-279463-bli8hwda	14	33	can	can	MD
cord-279463-bli8hwda	14	34	determine	determine	VB
cord-279463-bli8hwda	14	35	cleavage	cleavage	NN
cord-279463-bli8hwda	14	36	by	by	IN
cord-279463-bli8hwda	14	37	signal	signal	NN
cord-279463-bli8hwda	14	38	peptldase	peptldase	NN
cord-279463-bli8hwda	14	39	.	.	.
cord-279463-bli8hwda	15	1	Translocation	translocation	NN
cord-279463-bli8hwda	15	2	of	of	IN
cord-279463-bli8hwda	15	3	proteins	protein	NNS
cord-279463-bli8hwda	15	4	across	across	IN
cord-279463-bli8hwda	15	5	the	the	DT
cord-279463-bli8hwda	15	6	membrane	membrane	NN
cord-279463-bli8hwda	15	7	of	of	IN
cord-279463-bli8hwda	15	8	the	the	DT
cord-279463-bli8hwda	15	9	endoplasmic	endoplasmic	NN
cord-279463-bli8hwda	15	10	reticulum	reticulum	NN
cord-279463-bli8hwda	15	11	(	(	-LRB-
cord-279463-bli8hwda	15	12	ER	ER	NNP
cord-279463-bli8hwda	15	13	)	)	-RRB-
cord-279463-bli8hwda	15	14	requires	require	VBZ
cord-279463-bli8hwda	15	15	signal	signal	NN
cord-279463-bli8hwda	15	16	sequences	sequence	NNS
cord-279463-bli8hwda	15	17	and	and	CC
cord-279463-bli8hwda	15	18	specific	specific	JJ
cord-279463-bli8hwda	15	19	receptors	receptor	NNS
cord-279463-bli8hwda	15	20	that	that	WDT
cord-279463-bli8hwda	15	21	recognize	recognize	VBP
cord-279463-bli8hwda	15	22	them	-PRON-	PRP
cord-279463-bli8hwda	15	23	(	(	-LRB-
cord-279463-bli8hwda	15	24	see	see	VB
cord-279463-bli8hwda	15	25	recent	recent	JJ
cord-279463-bli8hwda	15	26	reviews	review	NNS
cord-279463-bli8hwda	15	27	by	by	IN
cord-279463-bli8hwda	15	28	Hortsch	Hortsch	NNP
cord-279463-bli8hwda	15	29	and	and	CC
cord-279463-bli8hwda	15	30	Meyer	Meyer	NNP
cord-279463-bli8hwda	15	31	,	,	,
cord-279463-bli8hwda	15	32	1984	1984	CD
cord-279463-bli8hwda	15	33	;	;	.
cord-279463-bli8hwda	16	1	Walter	Walter	NNP
cord-279463-bli8hwda	16	2	et	et	NNP
cord-279463-bli8hwda	16	3	al	al	NNP
cord-279463-bli8hwda	16	4	.	.	NNP
cord-279463-bli8hwda	16	5	,	,	,
cord-279463-bli8hwda	16	6	1984	1984	CD
cord-279463-bli8hwda	16	7	;	;	:
cord-279463-bli8hwda	16	8	Rapoport	Rapoport	NNP
cord-279463-bli8hwda	16	9	and	and	CC
cord-279463-bli8hwda	16	10	Wiedmann	Wiedmann	NNP
cord-279463-bli8hwda	16	11	,	,	,
cord-279463-bli8hwda	16	12	1985	1985	CD
cord-279463-bli8hwda	16	13	;	;	:
cord-279463-bli8hwda	16	14	Wickner	Wickner	NNP
cord-279463-bli8hwda	16	15	and	and	CC
cord-279463-bli8hwda	16	16	Lodish	Lodish	NNP
cord-279463-bli8hwda	16	17	,	,	,
cord-279463-bli8hwda	16	18	1985	1985	CD
cord-279463-bli8hwda	16	19	)	)	-RRB-
cord-279463-bli8hwda	16	20	.	.	.
cord-279463-bli8hwda	17	1	Signal	signal	NN
cord-279463-bli8hwda	17	2	sequences	sequence	NNS
cord-279463-bli8hwda	17	3	have	have	VBP
cord-279463-bli8hwda	17	4	been	be	VBN
cord-279463-bli8hwda	17	5	found	find	VBN
cord-279463-bli8hwda	17	6	at	at	IN
cord-279463-bli8hwda	17	7	the	the	DT
cord-279463-bli8hwda	17	8	amino	amino	NN
cord-279463-bli8hwda	17	9	-	-	HYPH
cord-279463-bli8hwda	17	10	terminal	terminal	NN
cord-279463-bli8hwda	17	11	end	end	NN
cord-279463-bli8hwda	17	12	of	of	IN
cord-279463-bli8hwda	17	13	precursors	precursor	NNS
cord-279463-bli8hwda	17	14	for	for	IN
cord-279463-bli8hwda	17	15	secretory	secretory	NN
cord-279463-bli8hwda	17	16	and	and	CC
cord-279463-bli8hwda	17	17	transmembrane	transmembrane	NN
cord-279463-bli8hwda	17	18	proteins	protein	NNS
cord-279463-bli8hwda	17	19	.	.	.
cord-279463-bli8hwda	18	1	In	in	IN
cord-279463-bli8hwda	18	2	many	many	JJ
cord-279463-bli8hwda	18	3	cases	case	NNS
cord-279463-bli8hwda	18	4	they	-PRON-	PRP
cord-279463-bli8hwda	18	5	are	be	VBP
cord-279463-bli8hwda	18	6	cleaved	cleave	VBN
cord-279463-bli8hwda	18	7	during	during	IN
cord-279463-bli8hwda	18	8	their	-PRON-	PRP$
cord-279463-bli8hwda	18	9	translocation	translocation	NN
cord-279463-bli8hwda	18	10	across	across	IN
cord-279463-bli8hwda	18	11	the	the	DT
cord-279463-bli8hwda	18	12	membrane	membrane	NN
cord-279463-bli8hwda	18	13	by	by	IN
cord-279463-bli8hwda	18	14	a	a	DT
cord-279463-bli8hwda	18	15	specific	specific	JJ
cord-279463-bli8hwda	18	16	protease	protease	NN
cord-279463-bli8hwda	18	17	(	(	-LRB-
cord-279463-bli8hwda	18	18	signal	signal	NN
cord-279463-bli8hwda	18	19	peptidase	peptidase	NN
cord-279463-bli8hwda	18	20	)	)	-RRB-
cord-279463-bli8hwda	18	21	.	.	.
cord-279463-bli8hwda	19	1	Signal	signal	NN
cord-279463-bli8hwda	19	2	sequences	sequence	NNS
cord-279463-bli8hwda	19	3	are	be	VBP
cord-279463-bli8hwda	19	4	quite	quite	RB
cord-279463-bli8hwda	19	5	variable	variable	JJ
cord-279463-bli8hwda	19	6	in	in	IN
cord-279463-bli8hwda	19	7	length	length	NN
cord-279463-bli8hwda	19	8	,	,	,
cord-279463-bli8hwda	19	9	ranging	range	VBG
cord-279463-bli8hwda	19	10	from	from	IN
cord-279463-bli8hwda	19	11	16	16	CD
cord-279463-bli8hwda	19	12	to	to	IN
cord-279463-bli8hwda	19	13	more	more	JJR
cord-279463-bli8hwda	19	14	than	than	IN
cord-279463-bli8hwda	19	15	50	50	CD
cord-279463-bli8hwda	19	16	amino	amino	NN
cord-279463-bli8hwda	19	17	acid	acid	NN
cord-279463-bli8hwda	19	18	residues	residue	NNS
cord-279463-bli8hwda	19	19	(	(	-LRB-
cord-279463-bli8hwda	19	20	von	von	NNP
cord-279463-bli8hwda	19	21	Heijne	Heijne	NNP
cord-279463-bli8hwda	19	22	,	,	,
cord-279463-bli8hwda	19	23	1983	1983	CD
cord-279463-bli8hwda	19	24	)	)	-RRB-
cord-279463-bli8hwda	19	25	.	.	.
cord-279463-bli8hwda	20	1	They	-PRON-	PRP
cord-279463-bli8hwda	20	2	all	all	DT
cord-279463-bli8hwda	20	3	have	have	VBP
cord-279463-bli8hwda	20	4	a	a	DT
cord-279463-bli8hwda	20	5	central	central	JJ
cord-279463-bli8hwda	20	6	core	core	NN
cord-279463-bli8hwda	20	7	of	of	IN
cord-279463-bli8hwda	20	8	hydrophobic	hydrophobic	JJ
cord-279463-bli8hwda	20	9	amino	amino	NN
cord-279463-bli8hwda	20	10	acid	acid	NN
cord-279463-bli8hwda	20	11	residues	residue	NNS
cord-279463-bli8hwda	20	12	,	,	,
cord-279463-bli8hwda	20	13	and	and	CC
cord-279463-bli8hwda	20	14	most	most	JJS
cord-279463-bli8hwda	20	15	of	of	IN
cord-279463-bli8hwda	20	16	them	-PRON-	PRP
cord-279463-bli8hwda	20	17	have	have	VBP
cord-279463-bli8hwda	20	18	a	a	DT
cord-279463-bli8hwda	20	19	positively	positively	RB
cord-279463-bli8hwda	20	20	charged	charge	VBN
cord-279463-bli8hwda	20	21	amino	amino	NN
cord-279463-bli8hwda	20	22	-	-	HYPH
cord-279463-bli8hwda	20	23	terminal	terminal	NN
cord-279463-bli8hwda	20	24	segment	segment	NN
cord-279463-bli8hwda	20	25	(	(	-LRB-
cord-279463-bli8hwda	20	26	von	von	NNP
cord-279463-bli8hwda	20	27	Heijne	Heijne	NNP
cord-279463-bli8hwda	20	28	,	,	,
cord-279463-bli8hwda	20	29	1985	1985	CD
cord-279463-bli8hwda	20	30	)	)	-RRB-
cord-279463-bli8hwda	20	31	.	.	.
cord-279463-bli8hwda	21	1	Signal	signal	NN
cord-279463-bli8hwda	21	2	sequences	sequence	NNS
cord-279463-bli8hwda	21	3	on	on	IN
cord-279463-bli8hwda	21	4	nascent	nascent	JJ
cord-279463-bli8hwda	21	5	polypeptides	polypeptide	NNS
cord-279463-bli8hwda	21	6	are	be	VBP
cord-279463-bli8hwda	21	7	recognized	recognize	VBN
cord-279463-bli8hwda	21	8	by	by	IN
cord-279463-bli8hwda	21	9	the	the	DT
cord-279463-bli8hwda	21	10	signal	signal	NN
cord-279463-bli8hwda	21	11	recognition	recognition	NN
cord-279463-bli8hwda	21	12	particle	particle	NN
cord-279463-bli8hwda	21	13	(	(	-LRB-
cord-279463-bli8hwda	21	14	SRP	SRP	NNP
cord-279463-bli8hwda	21	15	)	)	-RRB-
cord-279463-bli8hwda	21	16	,	,	,
cord-279463-bli8hwda	21	17	a	a	DT
cord-279463-bli8hwda	21	18	ribonucleoprotein	ribonucleoprotein	NN
cord-279463-bli8hwda	21	19	complex	complex	NN
cord-279463-bli8hwda	21	20	that	that	WDT
cord-279463-bli8hwda	21	21	mediates	mediate	VBZ
cord-279463-bli8hwda	21	22	the	the	DT
cord-279463-bli8hwda	21	23	interaction	interaction	NN
cord-279463-bli8hwda	21	24	with	with	IN
cord-279463-bli8hwda	21	25	the	the	DT
cord-279463-bli8hwda	21	26	membrane	membrane	NN
cord-279463-bli8hwda	21	27	by	by	IN
cord-279463-bli8hwda	21	28	the	the	DT
cord-279463-bli8hwda	21	29	selective	selective	JJ
cord-279463-bli8hwda	21	30	binding	binding	NN
cord-279463-bli8hwda	21	31	to	to	IN
cord-279463-bli8hwda	21	32	docking	docking	NN
cord-279463-bli8hwda	21	33	protein	protein	NN
cord-279463-bli8hwda	21	34	(	(	-LRB-
cord-279463-bli8hwda	21	35	or	or	CC
cord-279463-bli8hwda	21	36	SRP	SRP	NNP
cord-279463-bli8hwda	21	37	receptor	receptor	NN
cord-279463-bli8hwda	21	38	)	)	-RRB-
cord-279463-bli8hwda	22	1	(	(	-LRB-
cord-279463-bli8hwda	22	2	Walter	Walter	NNP
cord-279463-bli8hwda	22	3	et	et	NNP
cord-279463-bli8hwda	22	4	al	al	NNP
cord-279463-bli8hwda	22	5	.	.	NNP
cord-279463-bli8hwda	22	6	,	,	,
cord-279463-bli8hwda	22	7	1981b	1981b	CD
cord-279463-bli8hwda	22	8	;	;	:
cord-279463-bli8hwda	22	9	Meyer	Meyer	NNP
cord-279463-bli8hwda	22	10	et	et	NNP
cord-279463-bli8hwda	22	11	al	al	NNP
cord-279463-bli8hwda	22	12	.	.	NNP
cord-279463-bli8hwda	22	13	,	,	,
cord-279463-bli8hwda	22	14	1982	1982	CD
cord-279463-bli8hwda	23	1	;	;	:
cord-279463-bli8hwda	23	2	Gilmore	Gilmore	NNP
cord-279463-bli8hwda	23	3	et	et	NNP
cord-279463-bli8hwda	23	4	al	al	NNP
cord-279463-bli8hwda	23	5	.	.	NNP
cord-279463-bli8hwda	23	6	,	,	,
cord-279463-bli8hwda	23	7	1982	1982	CD
cord-279463-bli8hwda	23	8	)	)	-RRB-
cord-279463-bli8hwda	23	9	.	.	.
cord-279463-bli8hwda	24	1	Membrane	membrane	NN
cord-279463-bli8hwda	24	2	proteins	protein	NNS
cord-279463-bli8hwda	24	3	are	be	VBP
cord-279463-bli8hwda	24	4	also	also	RB
cord-279463-bli8hwda	24	5	inserted	insert	VBN
cord-279463-bli8hwda	24	6	into	into	IN
cord-279463-bli8hwda	24	7	the	the	DT
cord-279463-bli8hwda	24	8	ER	ER	NNP
cord-279463-bli8hwda	24	9	membrane	membrane	NN
cord-279463-bli8hwda	24	10	by	by	IN
cord-279463-bli8hwda	24	11	an	an	DT
cord-279463-bli8hwda	24	12	SRP	SRP	NNP
cord-279463-bli8hwda	24	13	-	-	HYPH
cord-279463-bli8hwda	24	14	mediated	mediate	VBN
cord-279463-bli8hwda	24	15	mechanism	mechanism	NN
cord-279463-bli8hwda	24	16	(	(	-LRB-
cord-279463-bli8hwda	24	17	Anderson	Anderson	NNP
cord-279463-bli8hwda	24	18	et	et	NNP
cord-279463-bli8hwda	24	19	al	al	NNP
cord-279463-bli8hwda	24	20	.	.	NNP
cord-279463-bli8hwda	24	21	,	,	,
cord-279463-bli8hwda	24	22	1983	1983	CD
cord-279463-bli8hwda	24	23	;	;	:
cord-279463-bli8hwda	24	24	Rottier	Rottier	NNP
cord-279463-bli8hwda	24	25	et	et	NNP
cord-279463-bli8hwda	24	26	al	al	NNP
cord-279463-bli8hwda	24	27	.	.	.
cord-279463-bli8hwda	25	1	,	,	,
cord-279463-bli8hwda	25	2	1985	1985	CD
cord-279463-bli8hwda	25	3	;	;	:
cord-279463-bli8hwda	25	4	Spiess	Spiess	NNP
cord-279463-bli8hwda	25	5	and	and	CC
cord-279463-bli8hwda	25	6	Lodish	Lodish	NNP
cord-279463-bli8hwda	25	7	,	,	,
cord-279463-bli8hwda	25	8	1986	1986	CD
cord-279463-bli8hwda	25	9	;	;	:
cord-279463-bli8hwda	25	10	Lipp	Lipp	NNP
cord-279463-bli8hwda	25	11	and	and	CC
cord-279463-bli8hwda	25	12	Dobberstein	Dobberstein	NNP
cord-279463-bli8hwda	25	13	,	,	,
cord-279463-bli8hwda	25	14	1986	1986	CD
cord-279463-bli8hwda	25	15	)	)	-RRB-
cord-279463-bli8hwda	25	16	.	.	.
cord-279463-bli8hwda	26	1	Those	those	DT
cord-279463-bli8hwda	26	2	spanning	span	VBG
cord-279463-bli8hwda	26	3	the	the	DT
cord-279463-bli8hwda	26	4	membrane	membrane	NN
cord-279463-bli8hwda	26	5	once	once	RB
cord-279463-bli8hwda	26	6	have	have	VBP
cord-279463-bli8hwda	26	7	either	either	CC
cord-279463-bli8hwda	26	8	the	the	DT
cord-279463-bli8hwda	26	9	carboxyl	carboxyl	NN
cord-279463-bli8hwda	26	10	terminus	terminus	NN
cord-279463-bli8hwda	26	11	(	(	-LRB-
cord-279463-bli8hwda	26	12	type	type	NN
cord-279463-bli8hwda	26	13	I	-PRON-	PRP
cord-279463-bli8hwda	26	14	membrane	membrane	NN
cord-279463-bli8hwda	26	15	proteins	protein	NNS
cord-279463-bli8hwda	26	16	)	)	-RRB-
cord-279463-bli8hwda	26	17	or	or	CC
cord-279463-bli8hwda	26	18	the	the	DT
cord-279463-bli8hwda	26	19	amino	amino	NN
cord-279463-bli8hwda	26	20	terminus	terminus	NN
cord-279463-bli8hwda	26	21	(	(	-LRB-
cord-279463-bli8hwda	26	22	type	type	NN
cord-279463-bli8hwda	26	23	II	II	NNP
cord-279463-bli8hwda	26	24	membrane	membrane	NN
cord-279463-bli8hwda	26	25	proteins	protein	NNS
cord-279463-bli8hwda	26	26	)	)	-RRB-
cord-279463-bli8hwda	26	27	exposed	expose	VBN
cord-279463-bli8hwda	26	28	on	on	IN
cord-279463-bli8hwda	26	29	the	the	DT
cord-279463-bli8hwda	26	30	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	26	31	side	side	NN
cord-279463-bli8hwda	26	32	.	.	.
cord-279463-bli8hwda	27	1	Membrane	membrane	NN
cord-279463-bli8hwda	27	2	insertion	insertion	NN
cord-279463-bli8hwda	27	3	of	of	IN
cord-279463-bli8hwda	27	4	type	type	NN
cord-279463-bli8hwda	27	5	I	-PRON-	PRP
cord-279463-bli8hwda	27	6	membrane	membrane	NN
cord-279463-bli8hwda	27	7	proteins	protein	NNS
cord-279463-bli8hwda	27	8	most	most	RBS
cord-279463-bli8hwda	27	9	likely	likely	JJ
cord-279463-bli8hwda	27	10	proceeds	proceed	VBZ
cord-279463-bli8hwda	27	11	in	in	IN
cord-279463-bli8hwda	27	12	a	a	DT
cord-279463-bli8hwda	27	13	manner	manner	NN
cord-279463-bli8hwda	27	14	very	very	RB
cord-279463-bli8hwda	27	15	similar	similar	JJ
cord-279463-bli8hwda	27	16	to	to	IN
cord-279463-bli8hwda	27	17	that	that	DT
cord-279463-bli8hwda	27	18	of	of	IN
cord-279463-bli8hwda	27	19	secretory	secretory	JJ
cord-279463-bli8hwda	27	20	proteins	protein	NNS
cord-279463-bli8hwda	28	1	(	(	-LRB-
cord-279463-bli8hwda	28	2	Lingappa	Lingappa	NNP
cord-279463-bli8hwda	28	3	et	et	NNP
cord-279463-bli8hwda	28	4	al	al	NNP
cord-279463-bli8hwda	28	5	.	.	NNP
cord-279463-bli8hwda	28	6	,	,	,
cord-279463-bli8hwda	28	7	1978	1978	CD
cord-279463-bli8hwda	28	8	)	)	-RRB-
cord-279463-bli8hwda	28	9	.	.	.
cord-279463-bli8hwda	29	1	Type	type	NN
cord-279463-bli8hwda	29	2	I	-PRON-	PRP
cord-279463-bli8hwda	29	3	membrane	membrane	NN
cord-279463-bli8hwda	29	4	proteins	protein	NNS
cord-279463-bli8hwda	29	5	are	be	VBP
cord-279463-bli8hwda	29	6	usually	usually	RB
cord-279463-bli8hwda	29	7	synthesized	synthesize	VBN
cord-279463-bli8hwda	29	8	with	with	IN
cord-279463-bli8hwda	29	9	a	a	DT
cord-279463-bli8hwda	29	10	cleavable	cleavable	JJ
cord-279463-bli8hwda	29	11	signal	signal	NN
cord-279463-bli8hwda	29	12	sequence	sequence	NN
cord-279463-bli8hwda	29	13	and	and	CC
cord-279463-bli8hwda	29	14	,	,	,
cord-279463-bli8hwda	29	15	in	in	IN
cord-279463-bli8hwda	29	16	contrast	contrast	NN
cord-279463-bli8hwda	29	17	to	to	IN
cord-279463-bli8hwda	29	18	secretory	secretory	JJ
cord-279463-bli8hwda	29	19	proteins	protein	NNS
cord-279463-bli8hwda	29	20	,	,	,
cord-279463-bli8hwda	29	21	are	be	VBP
cord-279463-bli8hwda	29	22	held	hold	VBN
cord-279463-bli8hwda	29	23	in	in	IN
cord-279463-bli8hwda	29	24	the	the	DT
cord-279463-bli8hwda	29	25	membrane	membrane	NN
cord-279463-bli8hwda	29	26	by	by	IN
cord-279463-bli8hwda	29	27	a	a	DT
cord-279463-bli8hwda	29	28	"	"	``
cord-279463-bli8hwda	29	29	stop	stop	NN
cord-279463-bli8hwda	29	30	transfer	transfer	NN
cord-279463-bli8hwda	29	31	"	"	''
cord-279463-bli8hwda	29	32	sequence	sequence	NN
cord-279463-bli8hwda	29	33	.	.	.
cord-279463-bli8hwda	30	1	Examples	example	NNS
cord-279463-bli8hwda	30	2	of	of	IN
cord-279463-bli8hwda	30	3	type	type	NN
cord-279463-bli8hwda	30	4	I	-PRON-	PRP
cord-279463-bli8hwda	30	5	membrane	membrane	NN
cord-279463-bli8hwda	30	6	proteins	protein	NNS
cord-279463-bli8hwda	30	7	are	be	VBP
cord-279463-bli8hwda	30	8	the	the	DT
cord-279463-bli8hwda	30	9	vesicular	vesicular	JJ
cord-279463-bli8hwda	30	10	stomatitis	stomatitis	NN
cord-279463-bli8hwda	30	11	virus	virus	NN
cord-279463-bli8hwda	30	12	G	g	NN
cord-279463-bli8hwda	30	13	protein	protein	NN
cord-279463-bli8hwda	30	14	and	and	CC
cord-279463-bli8hwda	30	15	class	class	NN
cord-279463-bli8hwda	30	16	I	i	NN
cord-279463-bli8hwda	30	17	and	and	CC
cord-279463-bli8hwda	30	18	class	class	NN
cord-279463-bli8hwda	30	19	II	II	NNP
cord-279463-bli8hwda	30	20	histocompatibility	histocompatibility	NN
cord-279463-bli8hwda	30	21	antigens	antigen	NNS
cord-279463-bli8hwda	30	22	(	(	-LRB-
cord-279463-bli8hwda	30	23	Lingappa	Lingappa	NNP
cord-279463-bli8hwda	30	24	et	et	NNP
cord-279463-bli8hwda	30	25	al	al	NNP
cord-279463-bli8hwda	30	26	.	.	NNP
cord-279463-bli8hwda	30	27	,	,	,
cord-279463-bli8hwda	30	28	1978	1978	CD
cord-279463-bli8hwda	30	29	;	;	:
cord-279463-bli8hwda	31	1	Dobberstein	Dobberstein	NNP
cord-279463-bli8hwda	31	2	et	et	NNP
cord-279463-bli8hwda	31	3	al	al	NNP
cord-279463-bli8hwda	31	4	.	.	NNP
cord-279463-bli8hwda	31	5	,	,	,
cord-279463-bli8hwda	31	6	1979	1979	CD
cord-279463-bli8hwda	31	7	)	)	-RRB-
cord-279463-bli8hwda	31	8	.	.	.
cord-279463-bli8hwda	32	1	Of	of	IN
cord-279463-bli8hwda	32	2	the	the	DT
cord-279463-bli8hwda	32	3	type	type	NN
cord-279463-bli8hwda	32	4	II	II	NNP
cord-279463-bli8hwda	32	5	membrane	membrane	NN
cord-279463-bli8hwda	32	6	proteins	protein	NNS
cord-279463-bli8hwda	32	7	so	so	RB
cord-279463-bli8hwda	32	8	far	far	RB
cord-279463-bli8hwda	32	9	investigated	investigate	VBN
cord-279463-bli8hwda	32	10	,	,	,
cord-279463-bli8hwda	32	11	all	all	DT
cord-279463-bli8hwda	32	12	are	be	VBP
cord-279463-bli8hwda	32	13	synthesized	synthesize	VBN
cord-279463-bli8hwda	32	14	without	without	IN
cord-279463-bli8hwda	32	15	a	a	DT
cord-279463-bli8hwda	32	16	cleavable	cleavable	JJ
cord-279463-bli8hwda	32	17	signal	signal	NN
cord-279463-bli8hwda	32	18	sequence	sequence	NN
cord-279463-bli8hwda	32	19	.	.	.
cord-279463-bli8hwda	33	1	The	the	DT
cord-279463-bli8hwda	33	2	neuraminidase	neuraminidase	NN
cord-279463-bli8hwda	33	3	of	of	IN
cord-279463-bli8hwda	33	4	influenzavirus	influenzavirus	NNP
cord-279463-bli8hwda	33	5	(	(	-LRB-
cord-279463-bli8hwda	33	6	60s	60	NNS
cord-279463-bli8hwda	33	7	et	et	NNP
cord-279463-bli8hwda	33	8	al	al	NNP
cord-279463-bli8hwda	33	9	.	.	NNP
cord-279463-bli8hwda	33	10	,	,	,
cord-279463-bli8hwda	33	11	1984	1984	CD
cord-279463-bli8hwda	33	12	)	)	-RRB-
cord-279463-bli8hwda	33	13	,	,	,
cord-279463-bli8hwda	33	14	the	the	DT
cord-279463-bli8hwda	33	15	invariant	invariant	JJ
cord-279463-bli8hwda	33	16	chain	chain	NN
cord-279463-bli8hwda	33	17	(	(	-LRB-
cord-279463-bli8hwda	33	18	Ii	Ii	NNP
cord-279463-bli8hwda	33	19	or	or	CC
cord-279463-bli8hwda	33	20	ly	ly	NNP
cord-279463-bli8hwda	33	21	)	)	-RRB-
cord-279463-bli8hwda	33	22	of	of	IN
cord-279463-bli8hwda	33	23	class	class	NN
cord-279463-bli8hwda	33	24	II	II	NNP
cord-279463-bli8hwda	33	25	histocompatibility	histocompatibility	NN
cord-279463-bli8hwda	33	26	antigens	antigen	NNS
cord-279463-bli8hwda	33	27	(	(	-LRB-
cord-279463-bli8hwda	33	28	Claesson	Claesson	NNP
cord-279463-bli8hwda	33	29	et	et	NNP
cord-279463-bli8hwda	33	30	al	al	NNP
cord-279463-bli8hwda	33	31	.	.	NNP
cord-279463-bli8hwda	33	32	,	,	,
cord-279463-bli8hwda	33	33	1983	1983	CD
cord-279463-bli8hwda	33	34	;	;	:
cord-279463-bli8hwda	33	35	Strubin	Strubin	NNP
cord-279463-bli8hwda	33	36	et	et	NNP
cord-279463-bli8hwda	33	37	al	al	NNP
cord-279463-bli8hwda	33	38	.	.	NNP
cord-279463-bli8hwda	33	39	,	,	,
cord-279463-bli8hwda	33	40	1984	1984	CD
cord-279463-bli8hwda	33	41	;	;	:
cord-279463-bli8hwda	33	42	Long	long	RB
cord-279463-bli8hwda	33	43	,	,	,
cord-279463-bli8hwda	33	44	1985	1985	CD
cord-279463-bli8hwda	33	45	;	;	:
cord-279463-bli8hwda	33	46	Lipp	Lipp	NNP
cord-279463-bli8hwda	33	47	and	and	CC
cord-279463-bli8hwda	33	48	Dobberstein	Dobberstein	NNP
cord-279463-bli8hwda	33	49	,	,	,
cord-279463-bli8hwda	33	50	1986	1986	CD
cord-279463-bli8hwda	33	51	)	)	-RRB-
cord-279463-bli8hwda	33	52	,	,	,
cord-279463-bli8hwda	33	53	the	the	DT
cord-279463-bli8hwda	33	54	transferrin	transferrin	NN
cord-279463-bli8hwda	33	55	receptor	receptor	NN
cord-279463-bli8hwda	33	56	(	(	-LRB-
cord-279463-bli8hwda	33	57	Schneider	Schneider	NNP
cord-279463-bli8hwda	33	58	et	et	NNP
cord-279463-bli8hwda	33	59	al	al	NNP
cord-279463-bli8hwda	33	60	.	.	NNP
cord-279463-bli8hwda	33	61	,	,	,
cord-279463-bli8hwda	33	62	1984	1984	CD
cord-279463-bli8hwda	33	63	)	)	-RRB-
cord-279463-bli8hwda	33	64	,	,	,
cord-279463-bli8hwda	33	65	and	and	CC
cord-279463-bli8hwda	33	66	the	the	DT
cord-279463-bli8hwda	33	67	asialoglycoprotein	asialoglycoprotein	NNP
cord-279463-bli8hwda	33	68	receptor	receptor	NN
cord-279463-bli8hwda	33	69	(	(	-LRB-
cord-279463-bli8hwda	33	70	Chiacchia	Chiacchia	NNP
cord-279463-bli8hwda	33	71	and	and	CC
cord-279463-bli8hwda	33	72	Drickamer	Drickamer	NNP
cord-279463-bli8hwda	33	73	,	,	,
cord-279463-bli8hwda	33	74	1984	1984	CD
cord-279463-bli8hwda	33	75	;	;	:
cord-279463-bli8hwda	33	76	Holland	Holland	NNP
cord-279463-bli8hwda	33	77	et	et	NNP
cord-279463-bli8hwda	33	78	al	al	NNP
cord-279463-bli8hwda	33	79	.	.	NNP
cord-279463-bli8hwda	33	80	,	,	,
cord-279463-bli8hwda	33	81	1984	1984	CD
cord-279463-bli8hwda	33	82	;	;	:
cord-279463-bli8hwda	33	83	Spiess	Spiess	NNP
cord-279463-bli8hwda	33	84	and	and	CC
cord-279463-bli8hwda	33	85	Lodish	Lodish	NNP
cord-279463-bli8hwda	33	86	,	,	,
cord-279463-bli8hwda	33	87	1986	1986	CD
cord-279463-bli8hwda	33	88	)	)	-RRB-
cord-279463-bli8hwda	33	89	all	all	DT
cord-279463-bli8hwda	33	90	belong	belong	VBP
cord-279463-bli8hwda	33	91	to	to	IN
cord-279463-bli8hwda	33	92	this	this	DT
cord-279463-bli8hwda	33	93	class	class	NN
cord-279463-bli8hwda	33	94	of	of	IN
cord-279463-bli8hwda	33	95	membrane	membrane	NN
cord-279463-bli8hwda	33	96	proteins	protein	NNS
cord-279463-bli8hwda	33	97	.	.	.
cord-279463-bli8hwda	34	1	Some	some	DT
cord-279463-bli8hwda	34	2	steps	step	NNS
cord-279463-bli8hwda	34	3	in	in	IN
cord-279463-bli8hwda	34	4	their	-PRON-	PRP$
cord-279463-bli8hwda	34	5	membrane	membrane	NN
cord-279463-bli8hwda	34	6	insertion	insertion	NN
cord-279463-bli8hwda	34	7	must	must	MD
cord-279463-bli8hwda	34	8	be	be	VB
cord-279463-bli8hwda	34	9	similar	similar	JJ
cord-279463-bli8hwda	34	10	to	to	IN
cord-279463-bli8hwda	34	11	that	that	DT
cord-279463-bli8hwda	34	12	of	of	IN
cord-279463-bli8hwda	34	13	secretory	secretory	NN
cord-279463-bli8hwda	34	14	and	and	CC
cord-279463-bli8hwda	34	15	type	type	NN
cord-279463-bli8hwda	34	16	I	-PRON-	PRP
cord-279463-bli8hwda	34	17	membrane	membrane	NN
cord-279463-bli8hwda	34	18	proteins	protein	NNS
cord-279463-bli8hwda	34	19	,	,	,
cord-279463-bli8hwda	34	20	as	as	IN
cord-279463-bli8hwda	34	21	an	an	DT
cord-279463-bli8hwda	34	22	SRP	SRP	NNP
cord-279463-bli8hwda	34	23	-	-	HYPH
cord-279463-bli8hwda	34	24	and	and	CC
cord-279463-bli8hwda	34	25	docking	dock	VBG
cord-279463-bli8hwda	34	26	protein	protein	NN
cord-279463-bli8hwda	34	27	-	-	HYPH
cord-279463-bli8hwda	34	28	dependent	dependent	JJ
cord-279463-bli8hwda	34	29	membrane	membrane	NN
cord-279463-bli8hwda	34	30	insertion	insertion	NN
cord-279463-bli8hwda	34	31	has	have	VBZ
cord-279463-bli8hwda	34	32	been	be	VBN
cord-279463-bli8hwda	34	33	demonstrated	demonstrate	VBN
cord-279463-bli8hwda	34	34	for	for	IN
cord-279463-bli8hwda	34	35	some	some	DT
cord-279463-bli8hwda	34	36	of	of	IN
cord-279463-bli8hwda	34	37	them	-PRON-	PRP
cord-279463-bli8hwda	35	1	(	(	-LRB-
cord-279463-bli8hwda	35	2	Spiess	Spiess	NNP
cord-279463-bli8hwda	35	3	and	and	CC
cord-279463-bli8hwda	35	4	Lodish	Lodish	NNP
cord-279463-bli8hwda	35	5	,	,	,
cord-279463-bli8hwda	35	6	1986	1986	CD
cord-279463-bli8hwda	35	7	;	;	:
cord-279463-bli8hwda	35	8	Lipp	Lipp	NNP
cord-279463-bli8hwda	35	9	and	and	CC
cord-279463-bli8hwda	35	10	Dobberstein	Dobberstein	NNP
cord-279463-bli8hwda	35	11	,	,	,
cord-279463-bli8hwda	35	12	1986	1986	CD
cord-279463-bli8hwda	35	13	)	)	-RRB-
cord-279463-bli8hwda	35	14	.	.	.
cord-279463-bli8hwda	36	1	Membrane	membrane	NN
cord-279463-bli8hwda	36	2	insertion	insertion	NN
cord-279463-bli8hwda	36	3	might	may	MD
cord-279463-bli8hwda	36	4	occur	occur	VB
cord-279463-bli8hwda	36	5	in	in	IN
cord-279463-bli8hwda	36	6	a	a	DT
cord-279463-bli8hwda	36	7	loop	loop	NN
cord-279463-bli8hwda	36	8	-	-	HYPH
cord-279463-bli8hwda	36	9	like	like	JJ
cord-279463-bli8hwda	36	10	fashion	fashion	NN
cord-279463-bli8hwda	36	11	as	as	IN
cord-279463-bli8hwda	36	12	this	this	DT
cord-279463-bli8hwda	36	13	scheme	scheme	NN
cord-279463-bli8hwda	36	14	can	can	MD
cord-279463-bli8hwda	36	15	most	most	RBS
cord-279463-bli8hwda	36	16	easily	easily	RB
cord-279463-bli8hwda	36	17	explain	explain	VB
cord-279463-bli8hwda	36	18	how	how	WRB
cord-279463-bli8hwda	36	19	the	the	DT
cord-279463-bli8hwda	36	20	different	different	JJ
cord-279463-bli8hwda	36	21	membrane	membrane	NN
cord-279463-bli8hwda	36	22	topologies	topology	NNS
cord-279463-bli8hwda	36	23	of	of	IN
cord-279463-bli8hwda	36	24	membrane	membrane	NN
cord-279463-bli8hwda	36	25	proteins	protein	NNS
cord-279463-bli8hwda	36	26	are	be	VBP
cord-279463-bli8hwda	36	27	achieved	achieve	VBN
cord-279463-bli8hwda	36	28	(	(	-LRB-
cord-279463-bli8hwda	36	29	Engelman	Engelman	NNP
cord-279463-bli8hwda	36	30	and	and	CC
cord-279463-bli8hwda	36	31	Steitz	Steitz	NNP
cord-279463-bli8hwda	36	32	,	,	,
cord-279463-bli8hwda	36	33	1981	1981	CD
cord-279463-bli8hwda	36	34	)	)	-RRB-
cord-279463-bli8hwda	36	35	.	.	.
cord-279463-bli8hwda	37	1	As	as	IN
cord-279463-bli8hwda	37	2	type	type	NN
cord-279463-bli8hwda	37	3	II	II	NNP
cord-279463-bli8hwda	37	4	membrane	membrane	NN
cord-279463-bli8hwda	37	5	proteins	protein	NNS
cord-279463-bli8hwda	37	6	contain	contain	VBP
cord-279463-bli8hwda	37	7	only	only	RB
cord-279463-bli8hwda	37	8	a	a	DT
cord-279463-bli8hwda	37	9	single	single	JJ
cord-279463-bli8hwda	37	10	stretch	stretch	NN
cord-279463-bli8hwda	37	11	of	of	IN
cord-279463-bli8hwda	37	12	hydrophobic	hydrophobic	JJ
cord-279463-bli8hwda	37	13	amino	amino	NN
cord-279463-bli8hwda	37	14	acid	acid	NN
cord-279463-bli8hwda	37	15	residues	residue	NNS
cord-279463-bli8hwda	37	16	,	,	,
cord-279463-bli8hwda	37	17	this	this	DT
cord-279463-bli8hwda	37	18	might	may	MD
cord-279463-bli8hwda	37	19	function	function	VB
cord-279463-bli8hwda	37	20	as	as	IN
cord-279463-bli8hwda	37	21	a	a	DT
cord-279463-bli8hwda	37	22	signal	signal	NN
cord-279463-bli8hwda	37	23	for	for	IN
cord-279463-bli8hwda	37	24	membrane	membrane	NN
cord-279463-bli8hwda	37	25	insertion	insertion	NN
cord-279463-bli8hwda	37	26	as	as	RB
cord-279463-bli8hwda	37	27	well	well	RB
cord-279463-bli8hwda	37	28	as	as	IN
cord-279463-bli8hwda	37	29	a	a	DT
cord-279463-bli8hwda	37	30	membrane	membrane	NN
cord-279463-bli8hwda	37	31	anchor	anchor	NN
cord-279463-bli8hwda	37	32	(	(	-LRB-
cord-279463-bli8hwda	37	33	Markoff	Markoff	NNP
cord-279463-bli8hwda	37	34	et	et	NNP
cord-279463-bli8hwda	37	35	al	al	NNP
cord-279463-bli8hwda	37	36	.	.	NNP
cord-279463-bli8hwda	37	37	,	,	,
cord-279463-bli8hwda	37	38	1984	1984	CD
cord-279463-bli8hwda	37	39	;	;	:
cord-279463-bli8hwda	37	40	Spiess	Spiess	NNP
cord-279463-bli8hwda	37	41	and	and	CC
cord-279463-bli8hwda	37	42	Lodish	Lodish	NNP
cord-279463-bli8hwda	37	43	,	,	,
cord-279463-bli8hwda	37	44	1986	1986	CD
cord-279463-bli8hwda	37	45	)	)	-RRB-
cord-279463-bli8hwda	37	46	.	.	.
cord-279463-bli8hwda	38	1	To	to	TO
cord-279463-bli8hwda	38	2	identify	identify	VB
cord-279463-bli8hwda	38	3	and	and	CC
cord-279463-bli8hwda	38	4	characterize	characterize	VB
cord-279463-bli8hwda	38	5	this	this	DT
cord-279463-bli8hwda	38	6	sequence	sequence	NN
cord-279463-bli8hwda	38	7	,	,	,
cord-279463-bli8hwda	38	8	we	-PRON-	PRP
cord-279463-bli8hwda	38	9	tested	test	VBD
cord-279463-bli8hwda	38	10	membrane	membrane	NN
cord-279463-bli8hwda	38	11	insertion	insertion	NN
cord-279463-bli8hwda	38	12	of	of	IN
cord-279463-bli8hwda	38	13	the	the	DT
cord-279463-bli8hwda	38	14	human	human	JJ
cord-279463-bli8hwda	38	15	invariant	invariant	JJ
cord-279463-bli8hwda	38	16	chain	chain	NN
cord-279463-bli8hwda	38	17	(	(	-LRB-
cord-279463-bli8hwda	38	18	ly	ly	NNP
cord-279463-bli8hwda	38	19	)	)	-RRB-
cord-279463-bli8hwda	38	20	and	and	CC
cord-279463-bli8hwda	38	21	several	several	JJ
cord-279463-bli8hwda	38	22	deletion	deletion	NN
cord-279463-bli8hwda	38	23	and	and	CC
cord-279463-bli8hwda	38	24	fusion	fusion	NN
cord-279463-bli8hwda	38	25	proteins	protein	NNS
cord-279463-bli8hwda	38	26	derived	derive	VBN
cord-279463-bli8hwda	38	27	from	from	IN
cord-279463-bli8hwda	38	28	it	-PRON-	PRP
cord-279463-bli8hwda	38	29	in	in	IN
cord-279463-bli8hwda	38	30	a	a	DT
cord-279463-bli8hwda	38	31	cell	cell	NN
cord-279463-bli8hwda	38	32	-	-	HYPH
cord-279463-bli8hwda	38	33	free	free	JJ
cord-279463-bli8hwda	38	34	membrane	membrane	NN
cord-279463-bli8hwda	38	35	insertion	insertion	NN
cord-279463-bli8hwda	38	36	system	system	NN
cord-279463-bli8hwda	38	37	.	.	.
cord-279463-bli8hwda	39	1	ly	ly	NNP
cord-279463-bli8hwda	39	2	is	be	VBZ
cord-279463-bli8hwda	39	3	a	a	DT
cord-279463-bli8hwda	39	4	typical	typical	JJ
cord-279463-bli8hwda	39	5	type	type	NN
cord-279463-bli8hwda	39	6	II	II	NNP
cord-279463-bli8hwda	39	7	membrane	membrane	NN
cord-279463-bli8hwda	39	8	protein	protein	NN
cord-279463-bli8hwda	39	9	(	(	-LRB-
cord-279463-bli8hwda	39	10	Claesson	Claesson	NNP
cord-279463-bli8hwda	39	11	et	et	NNP
cord-279463-bli8hwda	39	12	al	al	NNP
cord-279463-bli8hwda	39	13	.	.	NNP
cord-279463-bli8hwda	39	14	,	,	,
cord-279463-bli8hwda	39	15	1983	1983	CD
cord-279463-bli8hwda	39	16	;	;	:
cord-279463-bli8hwda	39	17	Strubin	Strubin	NNP
cord-279463-bli8hwda	39	18	et	et	NNP
cord-279463-bli8hwda	39	19	al	al	NNP
cord-279463-bli8hwda	39	20	.	.	NNP
cord-279463-bli8hwda	39	21	,	,	,
cord-279463-bli8hwda	39	22	1984	1984	CD
cord-279463-bli8hwda	39	23	;	;	:
cord-279463-bli8hwda	39	24	Lipp	Lipp	NNP
cord-279463-bli8hwda	39	25	and	and	CC
cord-279463-bli8hwda	39	26	Dobberstein	Dobberstein	NNP
cord-279463-bli8hwda	39	27	,	,	,
cord-279463-bli8hwda	39	28	1986	1986	CD
cord-279463-bli8hwda	39	29	)	)	-RRB-
cord-279463-bli8hwda	39	30	.	.	.
cord-279463-bli8hwda	40	1	It	-PRON-	PRP
cord-279463-bli8hwda	40	2	exposes	expose	VBZ
cord-279463-bli8hwda	40	3	30	30	CD
cord-279463-bli8hwda	40	4	amino	amino	NN
cord-279463-bli8hwda	40	5	-	-	HYPH
cord-279463-bli8hwda	40	6	terminal	terminal	NN
cord-279463-bli8hwda	40	7	residues	residue	NNS
cord-279463-bli8hwda	40	8	on	on	IN
cord-279463-bli8hwda	40	9	the	the	DT
cord-279463-bli8hwda	40	10	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	40	11	side	side	NN
cord-279463-bli8hwda	40	12	,	,	,
cord-279463-bli8hwda	40	13	spans	span	VBZ
cord-279463-bli8hwda	40	14	the	the	DT
cord-279463-bli8hwda	40	15	membrane	membrane	NN
cord-279463-bli8hwda	40	16	between	between	IN
cord-279463-bli8hwda	40	17	residues	residue	NNS
cord-279463-bli8hwda	40	18	30	30	CD
cord-279463-bli8hwda	40	19	and	and	CC
cord-279463-bli8hwda	40	20	60	60	CD
cord-279463-bli8hwda	40	21	,	,	,
cord-279463-bli8hwda	40	22	and	and	CC
cord-279463-bli8hwda	40	23	exposes	expose	VBZ
cord-279463-bli8hwda	40	24	a	a	DT
cord-279463-bli8hwda	40	25	large	large	JJ
cord-279463-bli8hwda	40	26	carboxy	carboxy	JJ
cord-279463-bli8hwda	40	27	-	-	HYPH
cord-279463-bli8hwda	40	28	terminal	terminal	NN
cord-279463-bli8hwda	40	29	domain	domain	NN
cord-279463-bli8hwda	40	30	on	on	IN
cord-279463-bli8hwda	40	31	the	the	DT
cord-279463-bli8hwda	40	32	exoplasmic	exoplasmic	JJ
cord-279463-bli8hwda	40	33	side	side	NN
cord-279463-bli8hwda	40	34	.	.	.
cord-279463-bli8hwda	41	1	This	this	DT
cord-279463-bli8hwda	41	2	domain	domain	NN
cord-279463-bli8hwda	41	3	has	have	VBZ
cord-279463-bli8hwda	41	4	two	two	CD
cord-279463-bli8hwda	41	5	sites	site	NNS
cord-279463-bli8hwda	41	6	for	for	IN
cord-279463-bli8hwda	41	7	the	the	DT
cord-279463-bli8hwda	41	8	addition	addition	NN
cord-279463-bli8hwda	41	9	of	of	IN
cord-279463-bli8hwda	41	10	N	n	NN
cord-279463-bli8hwda	41	11	-	-	HYPH
cord-279463-bli8hwda	41	12	linked	link	VBN
cord-279463-bli8hwda	41	13	carbohydrate	carbohydrate	NN
cord-279463-bli8hwda	41	14	units	unit	NNS
cord-279463-bli8hwda	41	15	.	.	.
cord-279463-bli8hwda	42	1	Membrane	membrane	NN
cord-279463-bli8hwda	42	2	insertion	insertion	NN
cord-279463-bli8hwda	42	3	of	of	IN
cord-279463-bli8hwda	42	4	ly	ly	NNP
cord-279463-bli8hwda	42	5	requires	require	VBZ
cord-279463-bli8hwda	42	6	SRP	SRP	NNP
cord-279463-bli8hwda	42	7	and	and	CC
cord-279463-bli8hwda	42	8	docking	docking	NN
cord-279463-bli8hwda	42	9	protein	protein	NN
cord-279463-bli8hwda	42	10	(	(	-LRB-
cord-279463-bli8hwda	42	11	Lipp	Lipp	NNP
cord-279463-bli8hwda	42	12	and	and	CC
cord-279463-bli8hwda	42	13	Dobberstein	Dobberstein	NNP
cord-279463-bli8hwda	42	14	,	,	,
cord-279463-bli8hwda	42	15	1986	1986	CD
cord-279463-bli8hwda	42	16	)	)	-RRB-
cord-279463-bli8hwda	42	17	.	.	.
cord-279463-bli8hwda	43	1	As	as	IN
cord-279463-bli8hwda	43	2	the	the	DT
cord-279463-bli8hwda	43	3	amino	amino	NN
cord-279463-bli8hwda	43	4	-	-	HYPH
cord-279463-bli8hwda	43	5	terminal	terminal	NN
cord-279463-bli8hwda	43	6	,	,	,
cord-279463-bli8hwda	43	7	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	43	8	domain	domain	NN
cord-279463-bli8hwda	43	9	is	be	VBZ
cord-279463-bli8hwda	43	10	hydrophilic	hydrophilic	JJ
cord-279463-bli8hwda	43	11	and	and	CC
cord-279463-bli8hwda	43	12	shows	show	VBZ
cord-279463-bli8hwda	43	13	no	no	DT
cord-279463-bli8hwda	43	14	resemblance	resemblance	NN
cord-279463-bli8hwda	43	15	to	to	IN
cord-279463-bli8hwda	43	16	a	a	DT
cord-279463-bli8hwda	43	17	signal	signal	JJ
cord-279463-bli8hwda	43	18	sequence	sequence	NN
cord-279463-bli8hwda	43	19	,	,	,
cord-279463-bli8hwda	43	20	it	-PRON-	PRP
cord-279463-bli8hwda	43	21	has	have	VBZ
cord-279463-bli8hwda	43	22	been	be	VBN
cord-279463-bli8hwda	43	23	proposed	propose	VBN
cord-279463-bli8hwda	43	24	that	that	IN
cord-279463-bli8hwda	43	25	the	the	DT
cord-279463-bli8hwda	43	26	membrane	membrane	NN
cord-279463-bli8hwda	43	27	-	-	HYPH
cord-279463-bli8hwda	43	28	spanning	span	VBG
cord-279463-bli8hwda	43	29	region	region	NN
cord-279463-bli8hwda	43	30	,	,	,
cord-279463-bli8hwda	43	31	or	or	CC
cord-279463-bli8hwda	43	32	part	part	NN
cord-279463-bli8hwda	43	33	of	of	IN
cord-279463-bli8hwda	43	34	it	-PRON-	PRP
cord-279463-bli8hwda	43	35	,	,	,
cord-279463-bli8hwda	43	36	functions	function	VBZ
cord-279463-bli8hwda	43	37	as	as	IN
cord-279463-bli8hwda	43	38	an	an	DT
cord-279463-bli8hwda	43	39	internal	internal	JJ
cord-279463-bli8hwda	43	40	,	,	,
cord-279463-bli8hwda	43	41	uncleavable	uncleavable	JJ
cord-279463-bli8hwda	43	42	signal	signal	NN
cord-279463-bli8hwda	43	43	sequence	sequence	NN
cord-279463-bli8hwda	43	44	(	(	-LRB-
cord-279463-bli8hwda	43	45	Dobberstein	Dobberstein	NNP
cord-279463-bli8hwda	43	46	et	et	NNP
cord-279463-bli8hwda	43	47	al	al	NNP
cord-279463-bli8hwda	43	48	.	.	NNP
cord-279463-bli8hwda	43	49	,	,	,
cord-279463-bli8hwda	43	50	1983	1983	CD
cord-279463-bli8hwda	43	51	;	;	:
cord-279463-bli8hwda	43	52	Claesson	Claesson	NNP
cord-279463-bli8hwda	43	53	et	et	NNP
cord-279463-bli8hwda	43	54	al	al	NNP
cord-279463-bli8hwda	43	55	.	.	NNP
cord-279463-bli8hwda	43	56	,	,	,
cord-279463-bli8hwda	43	57	1983	1983	CD
cord-279463-bli8hwda	43	58	;	;	:
cord-279463-bli8hwda	43	59	Lipp	Lipp	NNP
cord-279463-bli8hwda	43	60	and	and	CC
cord-279463-bli8hwda	43	61	Dobberstein	Dobberstein	NNP
cord-279463-bli8hwda	43	62	,	,	,
cord-279463-bli8hwda	43	63	1986	1986	CD
cord-279463-bli8hwda	43	64	)	)	-RRB-
cord-279463-bli8hwda	43	65	.	.	.
cord-279463-bli8hwda	44	1	We	-PRON-	PRP
cord-279463-bli8hwda	44	2	demonstrate	demonstrate	VBP
cord-279463-bli8hwda	44	3	here	here	RB
cord-279463-bli8hwda	44	4	that	that	IN
cord-279463-bli8hwda	44	5	the	the	DT
cord-279463-bli8hwda	44	6	membrane	membrane	NN
cord-279463-bli8hwda	44	7	-	-	HYPH
cord-279463-bli8hwda	44	8	spanning	span	VBG
cord-279463-bli8hwda	44	9	region	region	NN
cord-279463-bli8hwda	44	10	of	of	IN
cord-279463-bli8hwda	44	11	ly	ly	NNP
cord-279463-bli8hwda	44	12	is	be	VBZ
cord-279463-bli8hwda	44	13	composed	compose	VBN
cord-279463-bli8hwda	44	14	of	of	IN
cord-279463-bli8hwda	44	15	a	a	DT
cord-279463-bli8hwda	44	16	potentially	potentially	RB
cord-279463-bli8hwda	44	17	cleavable	cleavable	JJ
cord-279463-bli8hwda	44	18	signal	signal	NN
cord-279463-bli8hwda	44	19	sequence	sequence	NN
cord-279463-bli8hwda	44	20	fused	fuse	VBN
cord-279463-bli8hwda	44	21	to	to	IN
cord-279463-bli8hwda	44	22	part	part	NN
cord-279463-bli8hwda	44	23	of	of	IN
cord-279463-bli8hwda	44	24	a	a	DT
cord-279463-bli8hwda	44	25	membrane	membrane	NN
cord-279463-bli8hwda	44	26	anchor	anchor	NN
cord-279463-bli8hwda	44	27	,	,	,
cord-279463-bli8hwda	44	28	which	which	WDT
cord-279463-bli8hwda	44	29	together	together	RB
cord-279463-bli8hwda	44	30	with	with	IN
cord-279463-bli8hwda	44	31	the	the	DT
cord-279463-bli8hwda	44	32	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	44	33	domain	domain	NN
cord-279463-bli8hwda	44	34	determine	determine	VBP
cord-279463-bli8hwda	44	35	the	the	DT
cord-279463-bli8hwda	44	36	orientation	orientation	NN
cord-279463-bli8hwda	44	37	of	of	IN
cord-279463-bli8hwda	44	38	ly	ly	XX
cord-279463-bli8hwda	44	39	in	in	IN
cord-279463-bli8hwda	44	40	the	the	DT
cord-279463-bli8hwda	44	41	ER	ER	NNP
cord-279463-bli8hwda	44	42	membrane	membrane	NN
cord-279463-bli8hwda	44	43	.	.	.
cord-279463-bli8hwda	45	1	Deletion	deletion	NN
cord-279463-bli8hwda	45	2	of	of	IN
cord-279463-bli8hwda	45	3	the	the	DT
cord-279463-bli8hwda	45	4	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	45	5	domain	domain	NN
cord-279463-bli8hwda	45	6	exposes	expose	VBZ
cord-279463-bli8hwda	45	7	the	the	DT
cord-279463-bli8hwda	45	8	signal	signal	JJ
cord-279463-bli8hwda	45	9	sequence	sequence	NN
cord-279463-bli8hwda	45	10	at	at	IN
cord-279463-bli8hwda	45	11	the	the	DT
cord-279463-bli8hwda	45	12	amino	amino	NN
cord-279463-bli8hwda	45	13	terminus	terminus	NN
cord-279463-bli8hwda	45	14	of	of	IN
cord-279463-bli8hwda	45	15	the	the	DT
cord-279463-bli8hwda	45	16	membrane	membrane	NN
cord-279463-bli8hwda	45	17	-	-	HYPH
cord-279463-bli8hwda	45	18	spanning	span	VBG
cord-279463-bli8hwda	45	19	region	region	NN
cord-279463-bli8hwda	45	20	,	,	,
cord-279463-bli8hwda	45	21	resulting	result	VBG
cord-279463-bli8hwda	45	22	in	in	IN
cord-279463-bli8hwda	45	23	cleavage	cleavage	NN
cord-279463-bli8hwda	45	24	of	of	IN
cord-279463-bli8hwda	45	25	this	this	DT
cord-279463-bli8hwda	45	26	otherwise	otherwise	RB
cord-279463-bli8hwda	45	27	uncleaved	uncleaved	JJ
cord-279463-bli8hwda	45	28	signal	signal	NN
cord-279463-bli8hwda	45	29	.	.	.
cord-279463-bli8hwda	46	1	Claesson	Claesson	NNP
cord-279463-bli8hwda	46	2	et	et	NNP
cord-279463-bli8hwda	46	3	al	al	NNP
cord-279463-bli8hwda	46	4	.	.	NNP
cord-279463-bli8hwda	46	5	,	,	,
cord-279463-bli8hwda	46	6	1993	1993	CD
cord-279463-bli8hwda	46	7	)	)	-RRB-
cord-279463-bli8hwda	46	8	.	.	.
cord-279463-bli8hwda	47	1	ply	ply	VB
cord-279463-bli8hwda	47	2	.	.	.
cord-279463-bli8hwda	48	1	the	the	DT
cord-279463-bli8hwda	48	2	complete	complete	JJ
cord-279463-bli8hwda	48	3	ly	ly	XX
cord-279463-bli8hwda	48	4	coding	code	VBG
cord-279463-bli8hwda	48	5	and	and	CC
cord-279463-bli8hwda	48	6	all	all	DT
cord-279463-bli8hwda	48	7	of	of	IN
cord-279463-bli8hwda	48	8	its	-PRON-	PRP$
cord-279463-bli8hwda	48	9	3	3	CD
cord-279463-bli8hwda	48	10	'	'	POS
cord-279463-bli8hwda	48	11	noncoding	noncoding	JJ
cord-279463-bli8hwda	48	12	sequence	sequence	NN
cord-279463-bli8hwda	48	13	was	be	VBD
cord-279463-bli8hwda	48	14	cloned	clone	VBN
cord-279463-bli8hwda	48	15	behind	behind	IN
cord-279463-bli8hwda	48	16	the	the	DT
cord-279463-bli8hwda	48	17	T5	T5	NNP
cord-279463-bli8hwda	48	18	promoter	promoter	NN
cord-279463-bli8hwda	48	19	(	(	-LRB-
cord-279463-bli8hwda	48	20	P	p	NN
cord-279463-bli8hwda	48	21	)	)	-RRB-
cord-279463-bli8hwda	48	22	in	in	IN
cord-279463-bli8hwda	48	23	the	the	DT
cord-279463-bli8hwda	48	24	pDS5	pDS5	NNP
cord-279463-bli8hwda	48	25	expression	expression	NN
cord-279463-bli8hwda	48	26	vector	vector	NN
cord-279463-bli8hwda	48	27	.	.	.
cord-279463-bli8hwda	49	1	plycat	plycat	NNP
cord-279463-bli8hwda	49	2	,	,	,
cord-279463-bli8hwda	49	3	the	the	DT
cord-279463-bli8hwda	49	4	portion	portion	NN
cord-279463-bli8hwda	49	5	downstream	downstream	JJ
cord-279463-bli8hwda	49	6	of	of	IN
cord-279463-bli8hwda	49	7	the	the	DT
cord-279463-bli8hwda	49	8	Pstl	Pstl	NNP
cord-279463-bli8hwda	49	9	site	site	NN
cord-279463-bli8hwda	49	10	in	in	IN
cord-279463-bli8hwda	49	11	ply	ply	NN
cord-279463-bli8hwda	49	12	was	be	VBD
cord-279463-bli8hwda	49	13	replaced	replace	VBN
cord-279463-bli8hwda	49	14	by	by	IN
cord-279463-bli8hwda	49	15	the	the	DT
cord-279463-bli8hwda	49	16	chloramphenicol	chloramphenicol	NNP
cord-279463-bli8hwda	49	17	acetyltransferase	acetyltransferase	NNP
cord-279463-bli8hwda	49	18	(	(	-LRB-
cord-279463-bli8hwda	49	19	cat	cat	NN
cord-279463-bli8hwda	49	20	)	)	-RRB-
cord-279463-bli8hwda	49	21	gene	gene	NN
cord-279463-bli8hwda	49	22	resulting	result	VBG
cord-279463-bli8hwda	49	23	in	in	IN
cord-279463-bli8hwda	49	24	an	an	DT
cord-279463-bli8hwda	49	25	in	in	IN
cord-279463-bli8hwda	49	26	-	-	HYPH
cord-279463-bli8hwda	49	27	frame	frame	NN
cord-279463-bli8hwda	49	28	fusion	fusion	NN
cord-279463-bli8hwda	49	29	protein	protein	NN
cord-279463-bli8hwda	49	30	.	.	.
cord-279463-bli8hwda	50	1	PAN	pan	NN
cord-279463-bli8hwda	50	2	-	-	HYPH
cord-279463-bli8hwda	50	3	lycat	lycat	NN
cord-279463-bli8hwda	50	4	,	,	,
cord-279463-bli8hwda	50	5	the	the	DT
cord-279463-bli8hwda	50	6	segment	segment	NN
cord-279463-bli8hwda	50	7	between	between	IN
cord-279463-bli8hwda	50	8	the	the	DT
cord-279463-bli8hwda	50	9	Sau3A	Sau3A	NNS
cord-279463-bli8hwda	50	10	and	and	CC
cord-279463-bli8hwda	50	11	Sstll	Sstll	NNP
cord-279463-bli8hwda	50	12	sites	site	NNS
cord-279463-bli8hwda	50	13	of	of	IN
cord-279463-bli8hwda	50	14	plycat	plycat	NN
cord-279463-bli8hwda	50	15	coding	code	VBG
cord-279463-bli8hwda	50	16	for	for	IN
cord-279463-bli8hwda	50	17	the	the	DT
cord-279463-bli8hwda	50	18	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	50	19	domain	domain	NN
cord-279463-bli8hwda	50	20	was	be	VBD
cord-279463-bli8hwda	50	21	deleted	delete	VBN
cord-279463-bli8hwda	50	22	.	.	.
cord-279463-bli8hwda	51	1	A	a	DT
cord-279463-bli8hwda	51	2	new	new	JJ
cord-279463-bli8hwda	51	3	ATG	ATG	NNP
cord-279463-bli8hwda	51	4	initiation	initiation	NN
cord-279463-bli8hwda	51	5	codon	codon	NN
cord-279463-bli8hwda	51	6	right	right	RB
cord-279463-bli8hwda	51	7	in	in	IN
cord-279463-bli8hwda	51	8	front	front	NN
cord-279463-bli8hwda	51	9	of	of	IN
cord-279463-bli8hwda	51	10	the	the	DT
cord-279463-bli8hwda	51	11	membrane	membrane	NN
cord-279463-bli8hwda	51	12	-	-	HYPH
cord-279463-bli8hwda	51	13	spanning	span	VBG
cord-279463-bli8hwda	51	14	segment	segment	NN
cord-279463-bli8hwda	51	15	is	be	VBZ
cord-279463-bli8hwda	51	16	provided	provide	VBN
cord-279463-bli8hwda	51	17	by	by	IN
cord-279463-bli8hwda	51	18	the	the	DT
cord-279463-bli8hwda	51	19	vector	vector	NN
cord-279463-bli8hwda	51	20	(	(	-LRB-
cord-279463-bli8hwda	51	21	see	see	VB
cord-279463-bli8hwda	51	22	Figure	figure	NN
cord-279463-bli8hwda	51	23	4A	4a	CD
cord-279463-bli8hwda	51	24	)	)	-RRB-
cord-279463-bli8hwda	51	25	.	.	.
cord-279463-bli8hwda	52	1	The	the	DT
cord-279463-bli8hwda	52	2	regions	region	NNS
cord-279463-bli8hwda	52	3	coding	code	VBG
cord-279463-bli8hwda	52	4	for	for	IN
cord-279463-bli8hwda	52	5	protein	protein	NN
cord-279463-bli8hwda	52	6	are	be	VBP
cord-279463-bli8hwda	52	7	boxed	box	VBN
cord-279463-bli8hwda	52	8	.	.	.
cord-279463-bli8hwda	53	1	The	the	DT
cord-279463-bli8hwda	53	2	membrane	membrane	NN
cord-279463-bli8hwda	53	3	-	-	HYPH
cord-279463-bli8hwda	53	4	spanning	span	VBG
cord-279463-bli8hwda	53	5	region	region	NN
cord-279463-bli8hwda	53	6	of	of	IN
cord-279463-bli8hwda	53	7	ly	ly	NNP
cord-279463-bli8hwda	53	8	is	be	VBZ
cord-279463-bli8hwda	53	9	indicated	indicate	VBN
cord-279463-bli8hwda	53	10	by	by	IN
cord-279463-bli8hwda	53	11	loops	loop	NNS
cord-279463-bli8hwda	53	12	;	;	:
cord-279463-bli8hwda	53	13	the	the	DT
cord-279463-bli8hwda	53	14	hydrophilic	hydrophilic	JJ
cord-279463-bli8hwda	53	15	domains	domain	NNS
cord-279463-bli8hwda	53	16	by	by	IN
cord-279463-bli8hwda	53	17	dots	dot	NNS
cord-279463-bli8hwda	53	18	.	.	.
cord-279463-bli8hwda	54	1	cat	cat	NN
cord-279463-bli8hwda	54	2	-	-	HYPH
cord-279463-bli8hwda	54	3	derived	derive	VBN
cord-279463-bli8hwda	54	4	sequences	sequence	NNS
cord-279463-bli8hwda	54	5	are	be	VBP
cord-279463-bli8hwda	54	6	indicated	indicate	VBN
cord-279463-bli8hwda	54	7	by	by	IN
cord-279463-bli8hwda	54	8	slanted	slanted	JJ
cord-279463-bli8hwda	54	9	lines	line	NNS
cord-279463-bli8hwda	54	10	.	.	.
cord-279463-bli8hwda	55	1	The	the	DT
cord-279463-bli8hwda	55	2	position	position	NN
cord-279463-bli8hwda	55	3	of	of	IN
cord-279463-bli8hwda	55	4	N	n	NN
cord-279463-bli8hwda	55	5	-	-	HYPH
cord-279463-bli8hwda	55	6	linked	link	VBN
cord-279463-bli8hwda	55	7	glycosylation	glycosylation	NN
cord-279463-bli8hwda	55	8	sites	site	NNS
cord-279463-bli8hwda	55	9	in	in	IN
cord-279463-bli8hwda	55	10	ly	ly	NNP
cord-279463-bli8hwda	55	11	and	and	CC
cord-279463-bli8hwda	55	12	the	the	DT
cord-279463-bli8hwda	55	13	potential	potential	JJ
cord-279463-bli8hwda	55	14	N	n	CD
cord-279463-bli8hwda	55	15	-	-	HYPH
cord-279463-bli8hwda	55	16	linked	link	VBN
cord-279463-bli8hwda	55	17	glycosylation	glycosylation	NN
cord-279463-bli8hwda	55	18	site	site	NN
cord-279463-bli8hwda	55	19	in	in	IN
cord-279463-bli8hwda	55	20	CAT	cat	NN
cord-279463-bli8hwda	55	21	protein	protein	NN
cord-279463-bli8hwda	55	22	are	be	VBP
cord-279463-bli8hwda	55	23	indicated	indicate	VBN
cord-279463-bli8hwda	55	24	by	by	IN
cord-279463-bli8hwda	55	25	an	an	DT
cord-279463-bli8hwda	55	26	asterisk	asterisk	NN
cord-279463-bli8hwda	55	27	.	.	.
cord-279463-bli8hwda	56	1	Relevant	relevant	JJ
cord-279463-bli8hwda	56	2	cleavage	cleavage	NN
cord-279463-bli8hwda	56	3	sites	site	NNS
cord-279463-bli8hwda	56	4	for	for	IN
cord-279463-bli8hwda	56	5	restriction	restriction	NN
cord-279463-bli8hwda	56	6	endonucleases	endonuclease	NNS
cord-279463-bli8hwda	56	7	are	be	VBP
cord-279463-bli8hwda	56	8	also	also	RB
cord-279463-bli8hwda	56	9	indicated	indicate	VBN
cord-279463-bli8hwda	56	10	.	.	.
cord-279463-bli8hwda	57	1	Protein	protein	NN
cord-279463-bli8hwda	57	2	segments	segment	NNS
cord-279463-bli8hwda	57	3	that	that	WDT
cord-279463-bli8hwda	57	4	perform	perform	VBP
cord-279463-bli8hwda	57	5	a	a	DT
cord-279463-bli8hwda	57	6	particular	particular	JJ
cord-279463-bli8hwda	57	7	function	function	NN
cord-279463-bli8hwda	57	8	can	can	MD
cord-279463-bli8hwda	57	9	be	be	VB
cord-279463-bli8hwda	57	10	identified	identify	VBN
cord-279463-bli8hwda	57	11	by	by	IN
cord-279463-bli8hwda	57	12	their	-PRON-	PRP$
cord-279463-bli8hwda	57	13	deletion	deletion	NN
cord-279463-bli8hwda	57	14	or	or	CC
cord-279463-bli8hwda	57	15	addition	addition	NN
cord-279463-bli8hwda	57	16	to	to	IN
cord-279463-bli8hwda	57	17	unrelated	unrelated	JJ
cord-279463-bli8hwda	57	18	proteins	protein	NNS
cord-279463-bli8hwda	57	19	.	.	.
cord-279463-bli8hwda	58	1	We	-PRON-	PRP
cord-279463-bli8hwda	58	2	used	use	VBD
cord-279463-bli8hwda	58	3	this	this	DT
cord-279463-bli8hwda	58	4	approach	approach	NN
cord-279463-bli8hwda	58	5	to	to	TO
cord-279463-bli8hwda	58	6	localize	localize	VB
cord-279463-bli8hwda	58	7	and	and	CC
cord-279463-bli8hwda	58	8	characterize	characterize	VB
cord-279463-bli8hwda	58	9	the	the	DT
cord-279463-bli8hwda	58	10	region	region	NN
cord-279463-bli8hwda	58	11	in	in	IN
cord-279463-bli8hwda	58	12	ly	ly	XX
cord-279463-bli8hwda	58	13	that	that	DT
cord-279463-bli8hwda	58	14	is	be	VBZ
cord-279463-bli8hwda	58	15	responsible	responsible	JJ
cord-279463-bli8hwda	58	16	for	for	IN
cord-279463-bli8hwda	58	17	membrane	membrane	NN
cord-279463-bli8hwda	58	18	insertion	insertion	NN
cord-279463-bli8hwda	58	19	.	.	.
cord-279463-bli8hwda	59	1	Deletions	deletion	NNS
cord-279463-bli8hwda	59	2	and	and	CC
cord-279463-bli8hwda	59	3	fusions	fusion	NNS
cord-279463-bli8hwda	59	4	were	be	VBD
cord-279463-bli8hwda	59	5	made	make	VBN
cord-279463-bli8hwda	59	6	at	at	IN
cord-279463-bli8hwda	59	7	the	the	DT
cord-279463-bli8hwda	59	8	DNA	dna	NN
cord-279463-bli8hwda	59	9	level	level	NN
cord-279463-bli8hwda	59	10	after	after	IN
cord-279463-bli8hwda	59	11	cloning	clone	VBG
cord-279463-bli8hwda	59	12	of	of	IN
cord-279463-bli8hwda	59	13	ly	ly	NNP
cord-279463-bli8hwda	59	14	cDNA	cDNA	NNP
cord-279463-bli8hwda	59	15	into	into	IN
cord-279463-bli8hwda	59	16	an	an	DT
cord-279463-bli8hwda	59	17	expression	expression	NN
cord-279463-bli8hwda	59	18	vector	vector	NN
cord-279463-bli8hwda	59	19	.	.	.
cord-279463-bli8hwda	60	1	Messenger	Messenger	NNP
cord-279463-bli8hwda	60	2	RNA	RNA	NNP
cord-279463-bli8hwda	60	3	was	be	VBD
cord-279463-bli8hwda	60	4	transcribed	transcribe	VBN
cord-279463-bli8hwda	60	5	from	from	IN
cord-279463-bli8hwda	60	6	these	these	DT
cord-279463-bli8hwda	60	7	plasmids	plasmid	NNS
cord-279463-bli8hwda	60	8	and	and	CC
cord-279463-bli8hwda	60	9	translated	translate	VBN
cord-279463-bli8hwda	60	10	in	in	IN
cord-279463-bli8hwda	60	11	a	a	DT
cord-279463-bli8hwda	60	12	cell	cell	NN
cord-279463-bli8hwda	60	13	-	-	HYPH
cord-279463-bli8hwda	60	14	free	free	JJ
cord-279463-bli8hwda	60	15	system	system	NN
cord-279463-bli8hwda	60	16	.	.	.
cord-279463-bli8hwda	61	1	The	the	DT
cord-279463-bli8hwda	61	2	resulting	result	VBG
cord-279463-bli8hwda	61	3	proteins	protein	NNS
cord-279463-bli8hwda	61	4	were	be	VBD
cord-279463-bli8hwda	61	5	tested	test	VBN
cord-279463-bli8hwda	61	6	for	for	IN
cord-279463-bli8hwda	61	7	their	-PRON-	PRP$
cord-279463-bli8hwda	61	8	ability	ability	NN
cord-279463-bli8hwda	61	9	to	to	TO
cord-279463-bli8hwda	61	10	insert	insert	VB
cord-279463-bli8hwda	61	11	into	into	IN
cord-279463-bli8hwda	61	12	microsomal	microsomal	JJ
cord-279463-bli8hwda	61	13	membranes	membrane	NNS
cord-279463-bli8hwda	61	14	(	(	-LRB-
cord-279463-bli8hwda	61	15	Blobel	Blobel	NNP
cord-279463-bli8hwda	61	16	and	and	CC
cord-279463-bli8hwda	61	17	Dobberstein	Dobberstein	NNP
cord-279463-bli8hwda	61	18	,	,	,
cord-279463-bli8hwda	61	19	1975	1975	CD
cord-279463-bli8hwda	61	20	;	;	:
cord-279463-bli8hwda	61	21	Stueber	Stueber	NNP
cord-279463-bli8hwda	61	22	et	et	NNP
cord-279463-bli8hwda	61	23	al	al	NNP
cord-279463-bli8hwda	61	24	.	.	NNP
cord-279463-bli8hwda	61	25	,	,	,
cord-279463-bli8hwda	61	26	1984	1984	CD
cord-279463-bli8hwda	61	27	)	)	-RRB-
cord-279463-bli8hwda	61	28	.	.	.
cord-279463-bli8hwda	62	1	Plasmids	plasmid	NNS
cord-279463-bli8hwda	62	2	ply	ply	NN
cord-279463-bli8hwda	62	3	,	,	,
cord-279463-bli8hwda	62	4	plycat	plycat	NN
cord-279463-bli8hwda	62	5	,	,	,
cord-279463-bli8hwda	62	6	and	and	CC
cord-279463-bli8hwda	62	7	PAN	pan	NN
cord-279463-bli8hwda	62	8	-	-	HYPH
cord-279463-bli8hwda	62	9	lycat	lycat	NN
cord-279463-bli8hwda	63	1	We	-PRON-	PRP
cord-279463-bli8hwda	63	2	have	have	VBP
cord-279463-bli8hwda	63	3	shown	show	VBN
cord-279463-bli8hwda	63	4	previously	previously	RB
cord-279463-bli8hwda	63	5	that	that	IN
cord-279463-bli8hwda	63	6	cDNA	cdna	NN
cord-279463-bli8hwda	63	7	sequences	sequence	NNS
cord-279463-bli8hwda	63	8	cloned	clone	VBN
cord-279463-bli8hwda	63	9	behind	behind	IN
cord-279463-bli8hwda	63	10	the	the	DT
cord-279463-bli8hwda	63	11	strong	strong	JJ
cord-279463-bli8hwda	63	12	T5	T5	NNP
cord-279463-bli8hwda	63	13	promoter	promoter	NN
cord-279463-bli8hwda	63	14	in	in	IN
cord-279463-bli8hwda	63	15	pDS5	pDS5	NNP
cord-279463-bli8hwda	63	16	can	can	MD
cord-279463-bli8hwda	63	17	be	be	VB
cord-279463-bli8hwda	63	18	transcribed	transcribe	VBN
cord-279463-bli8hwda	63	19	very	very	RB
cord-279463-bli8hwda	63	20	efficiently	efficiently	RB
cord-279463-bli8hwda	63	21	by	by	IN
cord-279463-bli8hwda	63	22	E.	E.	NNP
cord-279463-bli8hwda	63	23	coli	coli	NNS
cord-279463-bli8hwda	63	24	RNA	rna	NN
cord-279463-bli8hwda	63	25	polymerase	polymerase	NN
cord-279463-bli8hwda	63	26	(	(	-LRB-
cord-279463-bli8hwda	63	27	Stueber	Stueber	NNP
cord-279463-bli8hwda	63	28	et	et	NNP
cord-279463-bli8hwda	63	29	al	al	NNP
cord-279463-bli8hwda	63	30	.	.	NNP
cord-279463-bli8hwda	63	31	,	,	,
cord-279463-bli8hwda	63	32	1985	1985	CD
cord-279463-bli8hwda	63	33	)	)	-RRB-
cord-279463-bli8hwda	63	34	.	.	.
cord-279463-bli8hwda	64	1	When	when	WRB
cord-279463-bli8hwda	64	2	transcription	transcription	NN
cord-279463-bli8hwda	64	3	is	be	VBZ
cord-279463-bli8hwda	64	4	performed	perform	VBN
cord-279463-bli8hwda	64	5	in	in	IN
cord-279463-bli8hwda	64	6	the	the	DT
cord-279463-bli8hwda	64	7	presence	presence	NN
cord-279463-bli8hwda	64	8	of	of	IN
cord-279463-bli8hwda	64	9	the	the	DT
cord-279463-bli8hwda	64	10	cap	cap	NN
cord-279463-bli8hwda	64	11	analog	analog	NN
cord-279463-bli8hwda	64	12	7mGpppA	7mgpppa	CD
cord-279463-bli8hwda	64	13	,	,	,
cord-279463-bli8hwda	64	14	the	the	DT
cord-279463-bli8hwda	64	15	resulting	result	VBG
cord-279463-bli8hwda	64	16	mRNA	mrna	NN
cord-279463-bli8hwda	64	17	can	can	MD
cord-279463-bli8hwda	64	18	be	be	VB
cord-279463-bli8hwda	64	19	translated	translate	VBN
cord-279463-bli8hwda	64	20	efficiently	efficiently	RB
cord-279463-bli8hwda	64	21	in	in	IN
cord-279463-bli8hwda	64	22	eukaryotic	eukaryotic	JJ
cord-279463-bli8hwda	64	23	cell	cell	NN
cord-279463-bli8hwda	64	24	-	-	HYPH
cord-279463-bli8hwda	64	25	free	free	JJ
cord-279463-bli8hwda	64	26	systems	system	NNS
cord-279463-bli8hwda	64	27	.	.	.
cord-279463-bli8hwda	65	1	We	-PRON-	PRP
cord-279463-bli8hwda	65	2	have	have	VBP
cord-279463-bli8hwda	65	3	observed	observe	VBN
cord-279463-bli8hwda	65	4	,	,	,
cord-279463-bli8hwda	65	5	however	however	RB
cord-279463-bli8hwda	65	6	,	,	,
cord-279463-bli8hwda	65	7	that	that	IN
cord-279463-bli8hwda	65	8	a	a	DT
cord-279463-bli8hwda	65	9	stretch	stretch	NN
cord-279463-bli8hwda	65	10	of	of	IN
cord-279463-bli8hwda	65	11	GC	GC	NNP
cord-279463-bli8hwda	65	12	residues	residue	NNS
cord-279463-bli8hwda	65	13	at	at	IN
cord-279463-bli8hwda	65	14	the	the	DT
cord-279463-bli8hwda	65	15	5	5	CD
cord-279463-bli8hwda	65	16	'	'	NN
cord-279463-bli8hwda	65	17	end	end	NN
cord-279463-bli8hwda	65	18	of	of	IN
cord-279463-bli8hwda	65	19	a	a	DT
cord-279463-bli8hwda	65	20	cDNA	cdna	NN
cord-279463-bli8hwda	65	21	negatively	negatively	RB
cord-279463-bli8hwda	65	22	affects	affect	VBZ
cord-279463-bli8hwda	65	23	expression	expression	NN
cord-279463-bli8hwda	65	24	of	of	IN
cord-279463-bli8hwda	65	25	the	the	DT
cord-279463-bli8hwda	65	26	resulting	result	VBG
cord-279463-bli8hwda	65	27	RNA	RNA	NNP
cord-279463-bli8hwda	65	28	(	(	-LRB-
cord-279463-bli8hwda	65	29	unpublished	unpublished	JJ
cord-279463-bli8hwda	65	30	observation	observation	NN
cord-279463-bli8hwda	65	31	)	)	-RRB-
cord-279463-bli8hwda	65	32	.	.	.
cord-279463-bli8hwda	66	1	The	the	DT
cord-279463-bli8hwda	66	2	ly	ly	XX
cord-279463-bli8hwda	66	3	cDNA	cdna	NN
cord-279463-bli8hwda	66	4	construct	construct	NN
cord-279463-bli8hwda	66	5	(	(	-LRB-
cord-279463-bli8hwda	66	6	py-2	py-2	NNP
cord-279463-bli8hwda	66	7	)	)	-RRB-
cord-279463-bli8hwda	66	8	had	have	VBD
cord-279463-bli8hwda	66	9	been	be	VBN
cord-279463-bli8hwda	66	10	GC	GC	NNP
cord-279463-bli8hwda	66	11	tailed	tail	VBN
cord-279463-bli8hwda	66	12	and	and	CC
cord-279463-bli8hwda	66	13	was	be	VBD
cord-279463-bli8hwda	66	14	inserted	insert	VBN
cord-279463-bli8hwda	66	15	into	into	IN
cord-279463-bli8hwda	66	16	the	the	DT
cord-279463-bli8hwda	66	17	Pstl	Pstl	NNP
cord-279463-bli8hwda	66	18	site	site	NN
cord-279463-bli8hwda	66	19	of	of	IN
cord-279463-bli8hwda	66	20	pBR322	pbr322	CD
cord-279463-bli8hwda	67	1	(	(	-LRB-
cord-279463-bli8hwda	67	2	Claesson	Claesson	NNP
cord-279463-bli8hwda	67	3	et	et	NNP
cord-279463-bli8hwda	67	4	al	al	NNP
cord-279463-bli8hwda	67	5	.	.	NNP
cord-279463-bli8hwda	67	6	,	,	,
cord-279463-bli8hwda	67	7	1983	1983	CD
cord-279463-bli8hwda	67	8	)	)	-RRB-
cord-279463-bli8hwda	67	9	.	.	.
cord-279463-bli8hwda	68	1	We	-PRON-	PRP
cord-279463-bli8hwda	68	2	deleted	delete	VBD
cord-279463-bli8hwda	68	3	the	the	DT
cord-279463-bli8hwda	68	4	5	5	CD
cord-279463-bli8hwda	68	5	'	'	''
cord-279463-bli8hwda	68	6	GC	GC	NNP
cord-279463-bli8hwda	68	7	tail	tail	NN
cord-279463-bli8hwda	68	8	and	and	CC
cord-279463-bli8hwda	68	9	cloned	clone	VBN
cord-279463-bli8hwda	68	10	ly	ly	XX
cord-279463-bli8hwda	68	11	cDNA	cdna	NN
cord-279463-bli8hwda	68	12	,	,	,
cord-279463-bli8hwda	68	13	or	or	CC
cord-279463-bli8hwda	68	14	part	part	NN
cord-279463-bli8hwda	68	15	of	of	IN
cord-279463-bli8hwda	68	16	it	-PRON-	PRP
cord-279463-bli8hwda	68	17	,	,	,
cord-279463-bli8hwda	68	18	into	into	IN
cord-279463-bli8hwda	68	19	the	the	DT
cord-279463-bli8hwda	68	20	polylinker	polylinker	NN
cord-279463-bli8hwda	68	21	site	site	NN
cord-279463-bli8hwda	68	22	of	of	IN
cord-279463-bli8hwda	68	23	pDS5	pDS5	NNP
cord-279463-bli8hwda	68	24	or	or	CC
cord-279463-bli8hwda	68	25	p6/5R	p6/5R	.
cord-279463-bli8hwda	68	26	(	(	-LRB-
cord-279463-bli8hwda	68	27	see	see	VB
cord-279463-bli8hwda	68	28	Experimental	Experimental	NNP
cord-279463-bli8hwda	68	29	Procedures	procedure	NNS
cord-279463-bli8hwda	68	30	for	for	IN
cord-279463-bli8hwda	68	31	details	detail	NNS
cord-279463-bli8hwda	68	32	)	)	-RRB-
cord-279463-bli8hwda	68	33	.	.	.
cord-279463-bli8hwda	69	1	ply	ply	NN
cord-279463-bli8hwda	69	2	contains	contain	VBZ
cord-279463-bli8hwda	69	3	the	the	DT
cord-279463-bli8hwda	69	4	entire	entire	JJ
cord-279463-bli8hwda	69	5	l	l	NNP
cord-279463-bli8hwda	69	6	-	-	HYPH
cord-279463-bli8hwda	69	7	y	y	NNP
cord-279463-bli8hwda	69	8	coding	code	VBG
cord-279463-bli8hwda	69	9	region	region	NN
cord-279463-bli8hwda	69	10	behind	behind	IN
cord-279463-bli8hwda	69	11	the	the	DT
cord-279463-bli8hwda	69	12	T5	T5	NNP
cord-279463-bli8hwda	69	13	promoter	promoter	NN
cord-279463-bli8hwda	69	14	(	(	-LRB-
cord-279463-bli8hwda	69	15	Figure	figure	NN
cord-279463-bli8hwda	69	16	1	1	CD
cord-279463-bli8hwda	69	17	)	)	-RRB-
cord-279463-bli8hwda	69	18	.	.	.
cord-279463-bli8hwda	70	1	plycat	plycat	NNP
cord-279463-bli8hwda	70	2	is	be	VBZ
cord-279463-bli8hwda	70	3	an	an	DT
cord-279463-bli8hwda	70	4	in	in	IN
cord-279463-bli8hwda	70	5	-	-	HYPH
cord-279463-bli8hwda	70	6	frame	frame	NN
cord-279463-bli8hwda	70	7	fusion	fusion	NN
cord-279463-bli8hwda	70	8	between	between	IN
cord-279463-bli8hwda	70	9	the	the	DT
cord-279463-bli8hwda	70	10	5	5	CD
cord-279463-bli8hwda	70	11	'	'	POS
cord-279463-bli8hwda	70	12	region	region	NN
cord-279463-bli8hwda	70	13	of	of	IN
cord-279463-bli8hwda	70	14	ly	ly	NNP
cord-279463-bli8hwda	70	15	encoding	encode	VBG
cord-279463-bli8hwda	70	16	the	the	DT
cord-279463-bli8hwda	70	17	cytoplas	cytopla	NNS
cord-279463-bli8hwda	70	18	,	,	,
cord-279463-bli8hwda	70	19	mic	mic	NN
cord-279463-bli8hwda	70	20	,	,	,
cord-279463-bli8hwda	70	21	membrane	membrane	NN
cord-279463-bli8hwda	70	22	-	-	HYPH
cord-279463-bli8hwda	70	23	spanning	span	VBG
cord-279463-bli8hwda	70	24	segment	segment	NN
cord-279463-bli8hwda	70	25	plus	plus	CC
cord-279463-bli8hwda	70	26	12	12	CD
cord-279463-bli8hwda	70	27	amino	amino	JJ
cord-279463-bli8hwda	70	28	acids	acid	NNS
cord-279463-bli8hwda	70	29	of	of	IN
cord-279463-bli8hwda	70	30	the	the	DT
cord-279463-bli8hwda	70	31	exoplasmic	exoplasmic	JJ
cord-279463-bli8hwda	70	32	portion	portion	NN
cord-279463-bli8hwda	70	33	of	of	IN
cord-279463-bli8hwda	70	34	ly	ly	NNP
cord-279463-bli8hwda	70	35	and	and	CC
cord-279463-bli8hwda	70	36	the	the	DT
cord-279463-bli8hwda	70	37	gene	gene	NN
cord-279463-bli8hwda	70	38	encoding	encode	VBG
cord-279463-bli8hwda	70	39	the	the	DT
cord-279463-bli8hwda	70	40	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	70	41	protein	protein	NN
cord-279463-bli8hwda	70	42	chloramphenicol	chloramphenicol	NNP
cord-279463-bli8hwda	70	43	acetyltransferase	acetyltransferase	NNP
cord-279463-bli8hwda	70	44	(	(	-LRB-
cord-279463-bli8hwda	70	45	CAT	CAT	NNP
cord-279463-bli8hwda	70	46	)	)	-RRB-
cord-279463-bli8hwda	70	47	.	.	.
cord-279463-bli8hwda	71	1	The	the	DT
cord-279463-bli8hwda	71	2	CAT	cat	NN
cord-279463-bli8hwda	71	3	protein	protein	NN
cord-279463-bli8hwda	71	4	contains	contain	VBZ
cord-279463-bli8hwda	71	5	one	one	CD
cord-279463-bli8hwda	71	6	potential	potential	JJ
cord-279463-bli8hwda	71	7	site	site	NN
cord-279463-bli8hwda	71	8	for	for	IN
cord-279463-bli8hwda	71	9	the	the	DT
cord-279463-bli8hwda	71	10	addition	addition	NN
cord-279463-bli8hwda	71	11	of	of	IN
cord-279463-bli8hwda	71	12	N	n	NN
cord-279463-bli8hwda	71	13	-	-	HYPH
cord-279463-bli8hwda	71	14	linked	link	VBN
cord-279463-bli8hwda	71	15	oligosaccharide	oligosaccharide	NN
cord-279463-bli8hwda	71	16	36	36	CD
cord-279463-bli8hwda	71	17	amino	amino	NN
cord-279463-bli8hwda	71	18	acid	acid	NN
cord-279463-bli8hwda	71	19	residues	residue	NNS
cord-279463-bli8hwda	71	20	downstream	downstream	RB
cord-279463-bli8hwda	71	21	of	of	IN
cord-279463-bli8hwda	71	22	its	-PRON-	PRP$
cord-279463-bli8hwda	71	23	original	original	JJ
cord-279463-bli8hwda	71	24	initiator	initiator	NN
cord-279463-bli8hwda	71	25	methionine	methionine	NN
cord-279463-bli8hwda	71	26	.	.	.
cord-279463-bli8hwda	72	1	In	in	IN
cord-279463-bli8hwda	72	2	AN	AN	NNP
cord-279463-bli8hwda	72	3	-	-	:
cord-279463-bli8hwda	72	4	IyCAT	IyCAT	NNP
cord-279463-bli8hwda	72	5	,	,	,
cord-279463-bli8hwda	72	6	the	the	DT
cord-279463-bli8hwda	72	7	entire	entire	JJ
cord-279463-bli8hwda	72	8	hydrophilic	hydrophilic	JJ
cord-279463-bli8hwda	72	9	,	,	,
cord-279463-bli8hwda	72	10	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	72	11	segment	segment	NN
cord-279463-bli8hwda	72	12	from	from	IN
cord-279463-bli8hwda	72	13	ly	ly	NNP
cord-279463-bli8hwda	72	14	was	be	VBD
cord-279463-bli8hwda	72	15	deleted	delete	VBN
cord-279463-bli8hwda	72	16	.	.	.
cord-279463-bli8hwda	73	1	The	the	DT
cord-279463-bli8hwda	73	2	new	new	JJ
cord-279463-bli8hwda	73	3	initiator	initiator	NN
cord-279463-bli8hwda	73	4	methionine	methionine	NN
cord-279463-bli8hwda	73	5	is	be	VBZ
cord-279463-bli8hwda	73	6	provided	provide	VBN
cord-279463-bli8hwda	73	7	by	by	IN
cord-279463-bli8hwda	73	8	the	the	DT
cord-279463-bli8hwda	73	9	vector	vector	NN
cord-279463-bli8hwda	73	10	and	and	CC
cord-279463-bli8hwda	73	11	is	be	VBZ
cord-279463-bli8hwda	73	12	located	locate	VBN
cord-279463-bli8hwda	73	13	in	in	IN
cord-279463-bli8hwda	73	14	front	front	NN
cord-279463-bli8hwda	73	15	of	of	IN
cord-279463-bli8hwda	73	16	the	the	DT
cord-279463-bli8hwda	73	17	hydrophobic	hydrophobic	JJ
cord-279463-bli8hwda	73	18	segment	segment	NN
cord-279463-bli8hwda	73	19	.	.	.
cord-279463-bli8hwda	74	1	In	in	IN
cord-279463-bli8hwda	74	2	Vitro	Vitro	NNP
cord-279463-bli8hwda	74	3	Translation	Translation	NNP
cord-279463-bli8hwda	74	4	and	and	CC
cord-279463-bli8hwda	74	5	Membrane	Membrane	NNP
cord-279463-bli8hwda	74	6	Insertion	insertion	NN
cord-279463-bli8hwda	74	7	of	of	IN
cord-279463-bli8hwda	74	8	ly	ly	XX
cord-279463-bli8hwda	75	1	When	when	WRB
cord-279463-bli8hwda	75	2	ply	ply	NN
cord-279463-bli8hwda	75	3	was	be	VBD
cord-279463-bli8hwda	75	4	transcribed	transcribe	VBN
cord-279463-bli8hwda	75	5	by	by	IN
cord-279463-bli8hwda	75	6	E.	E.	NNP
cord-279463-bli8hwda	75	7	coli	coli	NNP
cord-279463-bli8hwda	75	8	RNA	RNA	NNP
cord-279463-bli8hwda	75	9	polymerase	polymerase	NN
cord-279463-bli8hwda	75	10	and	and	CC
cord-279463-bli8hwda	75	11	the	the	DT
cord-279463-bli8hwda	75	12	resulting	result	VBG
cord-279463-bli8hwda	75	13	mRNA	mrna	NN
cord-279463-bli8hwda	75	14	translated	translate	VBN
cord-279463-bli8hwda	75	15	in	in	IN
cord-279463-bli8hwda	75	16	the	the	DT
cord-279463-bli8hwda	75	17	wheat	wheat	NN
cord-279463-bli8hwda	75	18	germ	germ	NN
cord-279463-bli8hwda	75	19	cell	cell	NN
cord-279463-bli8hwda	75	20	-	-	HYPH
cord-279463-bli8hwda	75	21	free	free	JJ
cord-279463-bli8hwda	75	22	system	system	NN
cord-279463-bli8hwda	75	23	,	,	,
cord-279463-bli8hwda	75	24	a	a	DT
cord-279463-bli8hwda	75	25	single	single	JJ
cord-279463-bli8hwda	75	26	polypeptide	polypeptide	NN
cord-279463-bli8hwda	75	27	species	species	NN
cord-279463-bli8hwda	75	28	of	of	IN
cord-279463-bli8hwda	75	29	27	27	CD
cord-279463-bli8hwda	75	30	kd	kd	NNS
cord-279463-bli8hwda	75	31	was	be	VBD
cord-279463-bli8hwda	75	32	obtained	obtain	VBN
cord-279463-bli8hwda	75	33	(	(	-LRB-
cord-279463-bli8hwda	75	34	Figure	figure	NN
cord-279463-bli8hwda	75	35	2	2	CD
cord-279463-bli8hwda	75	36	,	,	,
cord-279463-bli8hwda	75	37	lane	lane	NN
cord-279463-bli8hwda	75	38	1	1	CD
cord-279463-bli8hwda	75	39	)	)	-RRB-
cord-279463-bli8hwda	75	40	.	.	.
cord-279463-bli8hwda	76	1	This	this	DT
cord-279463-bli8hwda	76	2	is	be	VBZ
cord-279463-bli8hwda	76	3	the	the	DT
cord-279463-bli8hwda	76	4	expected	expect	VBN
cord-279463-bli8hwda	76	5	molecular	molecular	JJ
cord-279463-bli8hwda	76	6	weight	weight	NN
cord-279463-bli8hwda	76	7	for	for	IN
cord-279463-bli8hwda	76	8	nonglycosylated	nonglycosylated	NNP
cord-279463-bli8hwda	76	9	ly	ly	NNP
cord-279463-bli8hwda	76	10	(	(	-LRB-
cord-279463-bli8hwda	77	1	Claesson	Claesson	NNP
cord-279463-bli8hwda	77	2	et	et	NNP
cord-279463-bli8hwda	77	3	al	al	NNP
cord-279463-bli8hwda	77	4	.	.	NNP
cord-279463-bli8hwda	77	5	,	,	,
cord-279463-bli8hwda	77	6	1983	1983	CD
cord-279463-bli8hwda	77	7	)	)	-RRB-
cord-279463-bli8hwda	77	8	.	.	.
cord-279463-bli8hwda	78	1	When	when	WRB
cord-279463-bli8hwda	78	2	rough	rough	JJ
cord-279463-bli8hwda	78	3	microsomes	microsome	NNS
cord-279463-bli8hwda	78	4	(	(	-LRB-
cord-279463-bli8hwda	78	5	RM	RM	NNP
cord-279463-bli8hwda	78	6	)	)	-RRB-
cord-279463-bli8hwda	78	7	,	,	,
cord-279463-bli8hwda	78	8	derived	derive	VBN
cord-279463-bli8hwda	78	9	from	from	IN
cord-279463-bli8hwda	78	10	dog	dog	NN
cord-279463-bli8hwda	78	11	pancreas	pancrea	NNS
cord-279463-bli8hwda	78	12	,	,	,
cord-279463-bli8hwda	78	13	were	be	VBD
cord-279463-bli8hwda	78	14	added	add	VBN
cord-279463-bli8hwda	78	15	to	to	IN
cord-279463-bli8hwda	78	16	the	the	DT
cord-279463-bli8hwda	78	17	translation	translation	NN
cord-279463-bli8hwda	78	18	system	system	NN
cord-279463-bli8hwda	78	19	,	,	,
cord-279463-bli8hwda	78	20	a	a	DT
cord-279463-bli8hwda	78	21	higher	high	JJR
cord-279463-bli8hwda	78	22	molecular	molecular	JJ
cord-279463-bli8hwda	78	23	weight	weight	NN
cord-279463-bli8hwda	78	24	species	specie	NNS
cord-279463-bli8hwda	78	25	of	of	IN
cord-279463-bli8hwda	78	26	33	33	CD
cord-279463-bli8hwda	78	27	kd	kd	NNS
cord-279463-bli8hwda	78	28	appeared	appear	VBD
cord-279463-bli8hwda	78	29	.	.	.
cord-279463-bli8hwda	79	1	This	this	DT
cord-279463-bli8hwda	79	2	increase	increase	NN
cord-279463-bli8hwda	79	3	of	of	IN
cord-279463-bli8hwda	79	4	6	6	CD
cord-279463-bli8hwda	79	5	kd	kd	NNS
cord-279463-bli8hwda	79	6	in	in	IN
cord-279463-bli8hwda	79	7	molecular	molecular	JJ
cord-279463-bli8hwda	79	8	weight	weight	NN
cord-279463-bli8hwda	79	9	is	be	VBZ
cord-279463-bli8hwda	79	10	consistent	consistent	JJ
cord-279463-bli8hwda	79	11	with	with	IN
cord-279463-bli8hwda	79	12	the	the	DT
cord-279463-bli8hwda	79	13	addition	addition	NN
cord-279463-bli8hwda	79	14	of	of	IN
cord-279463-bli8hwda	79	15	two	two	CD
cord-279463-bli8hwda	79	16	oligosaccharides	oligosaccharide	NNS
cord-279463-bli8hwda	79	17	to	to	IN
cord-279463-bli8hwda	79	18	the	the	DT
cord-279463-bli8hwda	79	19	two	two	CD
cord-279463-bli8hwda	79	20	N	n	CD
cord-279463-bli8hwda	79	21	-	-	HYPH
cord-279463-bli8hwda	79	22	glycosylation	glycosylation	NN
cord-279463-bli8hwda	79	23	sites	site	NNS
cord-279463-bli8hwda	79	24	.	.	.
cord-279463-bli8hwda	80	1	The	the	DT
cord-279463-bli8hwda	80	2	33	33	CD
cord-279463-bli8hwda	80	3	kd	kd	NNP
cord-279463-bli8hwda	80	4	form	form	NN
cord-279463-bli8hwda	80	5	ly	ly	NNP
cord-279463-bli8hwda	80	6	*	*	NNP
cord-279463-bli8hwda	80	7	was	be	VBD
cord-279463-bli8hwda	80	8	reduced	reduce	VBN
cord-279463-bli8hwda	80	9	in	in	IN
cord-279463-bli8hwda	80	10	molecular	molecular	JJ
cord-279463-bli8hwda	80	11	weight	weight	NN
cord-279463-bli8hwda	80	12	by	by	IN
cord-279463-bli8hwda	80	13	about	about	RB
cord-279463-bli8hwda	80	14	2	2	CD
cord-279463-bli8hwda	80	15	kd	kd	NN
cord-279463-bli8hwda	80	16	when	when	WRB
cord-279463-bli8hwda	80	17	proteinase	proteinase	NN
cord-279463-bli8hwda	80	18	K	K	NNP
cord-279463-bli8hwda	80	19	was	be	VBD
cord-279463-bli8hwda	80	20	used	use	VBN
cord-279463-bli8hwda	80	21	to	to	TO
cord-279463-bli8hwda	80	22	remove	remove	VB
cord-279463-bli8hwda	80	23	the	the	DT
cord-279463-bli8hwda	80	24	cytoplasmically	cytoplasmically	RB
cord-279463-bli8hwda	80	25	exposed	expose	VBN
cord-279463-bli8hwda	80	26	domain	domain	NN
cord-279463-bli8hwda	80	27	(	(	-LRB-
cord-279463-bli8hwda	80	28	Figure	figure	NN
cord-279463-bli8hwda	80	29	2	2	CD
cord-279463-bli8hwda	80	30	,	,	,
cord-279463-bli8hwda	80	31	lanes	lane	NNS
cord-279463-bli8hwda	80	32	2	2	CD
cord-279463-bli8hwda	80	33	and	and	CC
cord-279463-bli8hwda	80	34	3	3	CD
cord-279463-bli8hwda	80	35	)	)	-RRB-
cord-279463-bli8hwda	80	36	.	.	.
cord-279463-bli8hwda	81	1	When	when	WRB
cord-279463-bli8hwda	81	2	protease	protease	JJ
cord-279463-bli8hwda	81	3	digestion	digestion	NN
cord-279463-bli8hwda	81	4	was	be	VBD
cord-279463-bli8hwda	81	5	performed	perform	VBN
cord-279463-bli8hwda	81	6	in	in	IN
cord-279463-bli8hwda	81	7	the	the	DT
cord-279463-bli8hwda	81	8	presence	presence	NN
cord-279463-bli8hwda	81	9	of	of	IN
cord-279463-bli8hwda	81	10	the	the	DT
cord-279463-bli8hwda	81	11	detergent	detergent	NN
cord-279463-bli8hwda	81	12	NP	NP	NNP
cord-279463-bli8hwda	81	13	40	40	CD
cord-279463-bli8hwda	81	14	,	,	,
cord-279463-bli8hwda	81	15	ly	ly	XX
cord-279463-bli8hwda	81	16	'	'	''
cord-279463-bli8hwda	81	17	was	be	VBD
cord-279463-bli8hwda	81	18	digested	digest	VBN
cord-279463-bli8hwda	81	19	.	.	.
cord-279463-bli8hwda	82	1	These	these	DT
cord-279463-bli8hwda	82	2	data	datum	NNS
cord-279463-bli8hwda	82	3	suggest	suggest	VBP
cord-279463-bli8hwda	82	4	that	that	IN
cord-279463-bli8hwda	82	5	ly	ly	XX
cord-279463-bli8hwda	82	6	'	'	''
cord-279463-bli8hwda	82	7	is	be	VBZ
cord-279463-bli8hwda	82	8	integrated	integrate	VBN
cord-279463-bli8hwda	82	9	into	into	IN
cord-279463-bli8hwda	82	10	the	the	DT
cord-279463-bli8hwda	82	11	membrane	membrane	NN
cord-279463-bli8hwda	82	12	and	and	CC
cord-279463-bli8hwda	82	13	exposes	expose	VBZ
cord-279463-bli8hwda	82	14	20	20	CD
cord-279463-bli8hwda	82	15	-	-	SYM
cord-279463-bli8hwda	82	16	30	30	CD
cord-279463-bli8hwda	82	17	amino	amino	NN
cord-279463-bli8hwda	82	18	acid	acid	NN
cord-279463-bli8hwda	82	19	residues	residue	NNS
cord-279463-bli8hwda	82	20	on	on	IN
cord-279463-bli8hwda	82	21	the	the	DT
cord-279463-bli8hwda	82	22	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	82	23	side	side	NN
cord-279463-bli8hwda	82	24	and	and	CC
cord-279463-bli8hwda	82	25	a	a	DT
cord-279463-bli8hwda	82	26	30	30	CD
cord-279463-bli8hwda	82	27	kd	kd	NNP
cord-279463-bli8hwda	82	28	domain	domain	NN
cord-279463-bli8hwda	82	29	on	on	IN
cord-279463-bli8hwda	82	30	the	the	DT
cord-279463-bli8hwda	82	31	exoplasmic	exoplasmic	JJ
cord-279463-bli8hwda	82	32	side	side	NN
cord-279463-bli8hwda	82	33	of	of	IN
cord-279463-bli8hwda	82	34	the	the	DT
cord-279463-bli8hwda	82	35	membrane	membrane	NN
cord-279463-bli8hwda	82	36	.	.	.
cord-279463-bli8hwda	83	1	The	the	DT
cord-279463-bli8hwda	83	2	identity	identity	NN
cord-279463-bli8hwda	83	3	of	of	IN
cord-279463-bli8hwda	83	4	ly	ly	NNS
cord-279463-bli8hwda	83	5	and	and	CC
cord-279463-bli8hwda	83	6	its	-PRON-	PRP$
cord-279463-bli8hwda	83	7	glycosylated	glycosylated	JJ
cord-279463-bli8hwda	83	8	form	form	NN
cord-279463-bli8hwda	83	9	was	be	VBD
cord-279463-bli8hwda	83	10	confirmed	confirm	VBN
cord-279463-bli8hwda	83	11	by	by	IN
cord-279463-bli8hwda	83	12	immunoprecipitation	immunoprecipitation	NN
cord-279463-bli8hwda	83	13	with	with	IN
cord-279463-bli8hwda	83	14	antibodies	antibody	NNS
cord-279463-bli8hwda	83	15	raised	raise	VBN
cord-279463-bli8hwda	83	16	against	against	IN
cord-279463-bli8hwda	83	17	the	the	DT
cord-279463-bli8hwda	83	18	amino	amino	NN
cord-279463-bli8hwda	83	19	-	-	HYPH
cord-279463-bli8hwda	83	20	terminal	terminal	NN
cord-279463-bli8hwda	83	21	72	72	CD
cord-279463-bli8hwda	83	22	(	(	-LRB-
cord-279463-bli8hwda	83	23	anti	anti	JJ
cord-279463-bli8hwda	83	24	-	-	JJ
cord-279463-bli8hwda	83	25	IyN	iyn	JJ
cord-279463-bli8hwda	83	26	)	)	-RRB-
cord-279463-bli8hwda	83	27	or	or	CC
cord-279463-bli8hwda	83	28	against	against	IN
cord-279463-bli8hwda	83	29	the	the	DT
cord-279463-bli8hwda	83	30	carboxy	carboxy	JJ
cord-279463-bli8hwda	83	31	-	-	HYPH
cord-279463-bli8hwda	83	32	terminal	terminal	NN
cord-279463-bli8hwda	83	33	144	144	CD
cord-279463-bli8hwda	83	34	(	(	-LRB-
cord-279463-bli8hwda	83	35	anti	anti	JJ
cord-279463-bli8hwda	83	36	-	-	JJ
cord-279463-bli8hwda	83	37	IyC	iyc	JJ
cord-279463-bli8hwda	83	38	)	)	-RRB-
cord-279463-bli8hwda	83	39	residues	residue	NNS
cord-279463-bli8hwda	83	40	of	of	IN
cord-279463-bli8hwda	83	41	ly	ly	XX
cord-279463-bli8hwda	83	42	.	.	.
cord-279463-bli8hwda	84	1	As	as	IN
cord-279463-bli8hwda	84	2	shown	show	VBN
cord-279463-bli8hwda	84	3	in	in	IN
cord-279463-bli8hwda	84	4	Figure	figure	NN
cord-279463-bli8hwda	84	5	2	2	CD
cord-279463-bli8hwda	84	6	,	,	,
cord-279463-bli8hwda	84	7	lanes	lane	NNS
cord-279463-bli8hwda	84	8	5	5	CD
cord-279463-bli8hwda	84	9	,	,	,
cord-279463-bli8hwda	84	10	6	6	CD
cord-279463-bli8hwda	84	11	,	,	,
cord-279463-bli8hwda	84	12	8	8	CD
cord-279463-bli8hwda	84	13	,	,	,
cord-279463-bli8hwda	84	14	and	and	CC
cord-279463-bli8hwda	84	15	9	9	CD
cord-279463-bli8hwda	84	16	,	,	,
cord-279463-bli8hwda	84	17	these	these	DT
cord-279463-bli8hwda	84	18	antibodies	antibody	NNS
cord-279463-bli8hwda	84	19	recognize	recognize	VBP
cord-279463-bli8hwda	84	20	glycosylated	glycosylate	VBN
cord-279463-bli8hwda	84	21	and	and	CC
cord-279463-bli8hwda	84	22	nonglycosylated	nonglycosylated	JJ
cord-279463-bli8hwda	84	23	forms	form	NNS
cord-279463-bli8hwda	84	24	of	of	IN
cord-279463-bli8hwda	84	25	ly	ly	XX
cord-279463-bli8hwda	84	26	.	.	.
cord-279463-bli8hwda	85	1	No	no	DT
cord-279463-bli8hwda	85	2	protein	protein	NN
cord-279463-bli8hwda	85	3	could	could	MD
cord-279463-bli8hwda	85	4	be	be	VB
cord-279463-bli8hwda	85	5	precipitated	precipitate	VBN
cord-279463-bli8hwda	85	6	with	with	IN
cord-279463-bli8hwda	85	7	anti	anti	JJ
cord-279463-bli8hwda	85	8	-	-	JJ
cord-279463-bli8hwda	85	9	IyN	iyn	JJ
cord-279463-bli8hwda	85	10	antibody	antibody	NN
cord-279463-bli8hwda	85	11	when	when	WRB
cord-279463-bli8hwda	85	12	the	the	DT
cord-279463-bli8hwda	85	13	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	85	14	domain	domain	NN
cord-279463-bli8hwda	85	15	was	be	VBD
cord-279463-bli8hwda	85	16	removed	remove	VBN
cord-279463-bli8hwda	85	17	from	from	IN
cord-279463-bli8hwda	85	18	membrane	membrane	NN
cord-279463-bli8hwda	85	19	-	-	HYPH
cord-279463-bli8hwda	85	20	integrated	integrate	VBN
cord-279463-bli8hwda	85	21	ly	ly	XX
cord-279463-bli8hwda	85	22	'	'	''
cord-279463-bli8hwda	85	23	by	by	IN
cord-279463-bli8hwda	85	24	protease	protease	NN
cord-279463-bli8hwda	85	25	digestion	digestion	NN
cord-279463-bli8hwda	85	26	(	(	-LRB-
cord-279463-bli8hwda	85	27	Figure	figure	NN
cord-279463-bli8hwda	85	28	2	2	CD
cord-279463-bli8hwda	85	29	,	,	,
cord-279463-bli8hwda	85	30	lane	lane	NN
cord-279463-bli8hwda	85	31	7	7	CD
cord-279463-bli8hwda	85	32	)	)	-RRB-
cord-279463-bli8hwda	85	33	.	.	.
cord-279463-bli8hwda	86	1	As	as	IN
cord-279463-bli8hwda	86	2	the	the	DT
cord-279463-bli8hwda	86	3	antibody	antibody	NN
cord-279463-bli8hwda	86	4	is	be	VBZ
cord-279463-bli8hwda	86	5	directed	direct	VBN
cord-279463-bli8hwda	86	6	against	against	IN
cord-279463-bli8hwda	86	7	the	the	DT
cord-279463-bli8hwda	86	8	amino	amino	NN
cord-279463-bli8hwda	86	9	-	-	HYPH
cord-279463-bli8hwda	86	10	terminal	terminal	NN
cord-279463-bli8hwda	86	11	portion	portion	NN
cord-279463-bli8hwda	86	12	of	of	IN
cord-279463-bli8hwda	86	13	ly	ly	XX
cord-279463-bli8hwda	86	14	,	,	,
cord-279463-bli8hwda	86	15	the	the	DT
cord-279463-bli8hwda	86	16	data	datum	NNS
cord-279463-bli8hwda	86	17	directly	directly	RB
cord-279463-bli8hwda	86	18	demonstrate	demonstrate	VBP
cord-279463-bli8hwda	86	19	that	that	IN
cord-279463-bli8hwda	86	20	the	the	DT
cord-279463-bli8hwda	86	21	amino	amino	NN
cord-279463-bli8hwda	86	22	terminus	terminus	NN
cord-279463-bli8hwda	86	23	is	be	VBZ
cord-279463-bli8hwda	86	24	located	locate	VBN
cord-279463-bli8hwda	86	25	on	on	IN
cord-279463-bli8hwda	86	26	the	the	DT
cord-279463-bli8hwda	86	27	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	86	28	side	side	NN
cord-279463-bli8hwda	86	29	and	and	CC
cord-279463-bli8hwda	86	30	is	be	VBZ
cord-279463-bli8hwda	86	31	accessible	accessible	JJ
cord-279463-bli8hwda	86	32	to	to	IN
cord-279463-bli8hwda	86	33	the	the	DT
cord-279463-bli8hwda	86	34	protease	protease	NN
cord-279463-bli8hwda	86	35	.	.	.
cord-279463-bli8hwda	87	1	With	with	IN
cord-279463-bli8hwda	87	2	anti	anti	JJ
cord-279463-bli8hwda	87	3	-	-	JJ
cord-279463-bli8hwda	87	4	IyC	iyc	JJ
cord-279463-bli8hwda	87	5	antibody	antibody	NN
cord-279463-bli8hwda	87	6	,	,	,
cord-279463-bli8hwda	87	7	the	the	DT
cord-279463-bli8hwda	87	8	processed	process	VBN
cord-279463-bli8hwda	87	9	form	form	NN
cord-279463-bli8hwda	87	10	of	of	IN
cord-279463-bli8hwda	87	11	ly	ly	NN
cord-279463-bli8hwda	87	12	is	be	VBZ
cord-279463-bli8hwda	87	13	readily	readily	RB
cord-279463-bli8hwda	87	14	detectable	detectable	JJ
cord-279463-bli8hwda	87	15	,	,	,
cord-279463-bli8hwda	87	16	demonstrating	demonstrate	VBG
cord-279463-bli8hwda	87	17	an	an	DT
cord-279463-bli8hwda	87	18	exoplasmic	exoplasmic	JJ
cord-279463-bli8hwda	87	19	location	location	NN
cord-279463-bli8hwda	87	20	of	of	IN
cord-279463-bli8hwda	87	21	the	the	DT
cord-279463-bli8hwda	87	22	carboxy	carboxy	JJ
cord-279463-bli8hwda	87	23	-	-	HYPH
cord-279463-bli8hwda	87	24	terminal	terminal	NN
cord-279463-bli8hwda	87	25	portion	portion	NN
cord-279463-bli8hwda	87	26	of	of	IN
cord-279463-bli8hwda	87	27	ly	ly	XX
cord-279463-bli8hwda	87	28	(	(	-LRB-
cord-279463-bli8hwda	87	29	Figure	Figure	NNP
cord-279463-bli8hwda	87	30	2	2	CD
cord-279463-bli8hwda	87	31	,	,	,
cord-279463-bli8hwda	87	32	lane	lane	NN
cord-279463-bli8hwda	87	33	10	10	CD
cord-279463-bli8hwda	87	34	)	)	-RRB-
cord-279463-bli8hwda	87	35	.	.	.
cord-279463-bli8hwda	88	1	Membrane	membrane	NN
cord-279463-bli8hwda	88	2	Insertion	insertion	NN
cord-279463-bli8hwda	88	3	of	of	IN
cord-279463-bli8hwda	88	4	IyCAT	IyCAT	NNP
cord-279463-bli8hwda	89	1	An	an	DT
cord-279463-bli8hwda	89	2	analysis	analysis	NN
cord-279463-bli8hwda	89	3	of	of	IN
cord-279463-bli8hwda	89	4	membrane	membrane	NN
cord-279463-bli8hwda	89	5	insertion	insertion	NN
cord-279463-bli8hwda	89	6	was	be	VBD
cord-279463-bli8hwda	89	7	performed	perform	VBN
cord-279463-bli8hwda	89	8	for	for	IN
cord-279463-bli8hwda	89	9	ly	ly	JJ
cord-279463-bli8hwda	89	10	-	-	NN
cord-279463-bli8hwda	89	11	CAT	cat	NN
cord-279463-bli8hwda	89	12	and	and	CC
cord-279463-bli8hwda	89	13	CAT	CAT	NNP
cord-279463-bli8hwda	89	14	as	as	IN
cord-279463-bli8hwda	89	15	described	describe	VBN
cord-279463-bli8hwda	89	16	above	above	RB
cord-279463-bli8hwda	89	17	for	for	IN
cord-279463-bli8hwda	89	18	ly	ly	XX
cord-279463-bli8hwda	89	19	.	.	.
cord-279463-bli8hwda	90	1	CAT	cat	NN
cord-279463-bli8hwda	90	2	was	be	VBD
cord-279463-bli8hwda	90	3	expressed	express	VBN
cord-279463-bli8hwda	90	4	from	from	IN
cord-279463-bli8hwda	90	5	pDS5	pDS5	NNP
cord-279463-bli8hwda	90	6	.	.	.
cord-279463-bli8hwda	91	1	IyCAT	IyCAT	NNP
cord-279463-bli8hwda	91	2	was	be	VBD
cord-279463-bli8hwda	91	3	synthesized	synthesize	VBN
cord-279463-bli8hwda	91	4	in	in	IN
cord-279463-bli8hwda	91	5	the	the	DT
cord-279463-bli8hwda	91	6	absence	absence	NN
cord-279463-bli8hwda	91	7	of	of	IN
cord-279463-bli8hwda	91	8	microsomal	microsomal	NNP
cord-279463-bli8hwda	91	9	membranes	membrane	NNS
cord-279463-bli8hwda	91	10	as	as	IN
cord-279463-bli8hwda	91	11	a	a	DT
cord-279463-bli8hwda	91	12	34	34	CD
cord-279463-bli8hwda	91	13	kd	kd	NNP
cord-279463-bli8hwda	91	14	protein	protein	NN
cord-279463-bli8hwda	91	15	(	(	-LRB-
cord-279463-bli8hwda	91	16	Figure	Figure	NNP
cord-279463-bli8hwda	91	17	3	3	CD
cord-279463-bli8hwda	91	18	,	,	,
cord-279463-bli8hwda	91	19	lane	lane	NN
cord-279463-bli8hwda	91	20	1	1	CD
cord-279463-bli8hwda	91	21	)	)	-RRB-
cord-279463-bli8hwda	91	22	and	and	CC
cord-279463-bli8hwda	91	23	in	in	IN
cord-279463-bli8hwda	91	24	the	the	DT
cord-279463-bli8hwda	91	25	presence	presence	NN
cord-279463-bli8hwda	91	26	of	of	IN
cord-279463-bli8hwda	91	27	microsomal	microsomal	NNP
cord-279463-bli8hwda	91	28	membranes	membrane	NNS
cord-279463-bli8hwda	91	29	as	as	IN
cord-279463-bli8hwda	91	30	a	a	DT
cord-279463-bli8hwda	91	31	37	37	CD
cord-279463-bli8hwda	91	32	kd	kd	NNP
cord-279463-bli8hwda	91	33	protein	protein	NN
cord-279463-bli8hwda	91	34	called	call	VBD
cord-279463-bli8hwda	91	35	lyCAT	lycat	FW
cord-279463-bli8hwda	91	36	*	*	NFP
cord-279463-bli8hwda	91	37	(	(	-LRB-
cord-279463-bli8hwda	91	38	Figure	figure	NN
cord-279463-bli8hwda	91	39	3	3	CD
cord-279463-bli8hwda	91	40	,	,	,
cord-279463-bli8hwda	91	41	lane	lane	NN
cord-279463-bli8hwda	91	42	2	2	CD
cord-279463-bli8hwda	91	43	)	)	-RRB-
cord-279463-bli8hwda	91	44	.	.	.
cord-279463-bli8hwda	92	1	12	12	CD
cord-279463-bli8hwda	92	2	3	3	CD
cord-279463-bli8hwda	92	3	4	4	CD
cord-279463-bli8hwda	93	1	In	in	IN
cord-279463-bli8hwda	93	2	Vitro	Vitro	NNP
cord-279463-bli8hwda	93	3	Translation	Translation	NNP
cord-279463-bli8hwda	93	4	and	and	CC
cord-279463-bli8hwda	93	5	Membrane	Membrane	NNP
cord-279463-bli8hwda	93	6	Insertion	insertion	NN
cord-279463-bli8hwda	93	7	of	of	IN
cord-279463-bli8hwda	93	8	ly	ly	XX
cord-279463-bli8hwda	93	9	ply	ply	NN
cord-279463-bli8hwda	93	10	was	be	VBD
cord-279463-bli8hwda	93	11	transcribed	transcribe	VBN
cord-279463-bli8hwda	93	12	in	in	IN
cord-279463-bli8hwda	93	13	the	the	DT
cord-279463-bli8hwda	93	14	presence	presence	NN
cord-279463-bli8hwda	93	15	of	of	IN
cord-279463-bli8hwda	93	16	the	the	DT
cord-279463-bli8hwda	93	17	cap	cap	NN
cord-279463-bli8hwda	93	18	analog	analog	NN
cord-279463-bli8hwda	93	19	7mGpppA	7mgpppa	CD
cord-279463-bli8hwda	93	20	by	by	IN
cord-279463-bli8hwda	93	21	E.	E.	NNP
cord-279463-bli8hwda	93	22	coli	coli	NNS
cord-279463-bli8hwda	93	23	RNA	RNA	NNP
cord-279463-bli8hwda	93	24	polymerase	polymerase	NN
cord-279463-bli8hwda	93	25	.	.	.
cord-279463-bli8hwda	94	1	The	the	DT
cord-279463-bli8hwda	94	2	resulting	result	VBG
cord-279463-bli8hwda	94	3	mRNA	mrna	NN
cord-279463-bli8hwda	94	4	was	be	VBD
cord-279463-bli8hwda	94	5	translated	translate	VBN
cord-279463-bli8hwda	94	6	in	in	IN
cord-279463-bli8hwda	94	7	the	the	DT
cord-279463-bli8hwda	94	8	wheat	wheat	NN
cord-279463-bli8hwda	94	9	germ	germ	NN
cord-279463-bli8hwda	94	10	cell	cell	NN
cord-279463-bli8hwda	94	11	-	-	HYPH
cord-279463-bli8hwda	94	12	free	free	JJ
cord-279463-bli8hwda	94	13	system	system	NN
cord-279463-bli8hwda	94	14	in	in	IN
cord-279463-bli8hwda	94	15	the	the	DT
cord-279463-bli8hwda	94	16	absence	absence	NN
cord-279463-bli8hwda	94	17	(	(	-LRB-
cord-279463-bli8hwda	94	18	lanes	lane	NNS
cord-279463-bli8hwda	94	19	1,5	1,5	CD
cord-279463-bli8hwda	94	20	.	.	.
cord-279463-bli8hwda	95	1	and	and	CC
cord-279463-bli8hwda	95	2	8)	8)	NN
cord-279463-bli8hwda	95	3	or	or	CC
cord-279463-bli8hwda	95	4	presence	presence	NN
cord-279463-bli8hwda	95	5	(	(	-LRB-
cord-279463-bli8hwda	95	6	lanes	lane	NNS
cord-279463-bli8hwda	95	7	2	2	CD
cord-279463-bli8hwda	95	8	,	,	,
cord-279463-bli8hwda	95	9	3	3	CD
cord-279463-bli8hwda	95	10	,	,	,
cord-279463-bli8hwda	95	11	4	4	CD
cord-279463-bli8hwda	95	12	,	,	,
cord-279463-bli8hwda	95	13	6	6	CD
cord-279463-bli8hwda	95	14	,	,	,
cord-279463-bli8hwda	95	15	7	7	CD
cord-279463-bli8hwda	95	16	,	,	,
cord-279463-bli8hwda	95	17	9	9	CD
cord-279463-bli8hwda	95	18	.	.	.
cord-279463-bli8hwda	95	19	and	and	CC
cord-279463-bli8hwda	95	20	10	10	CD
cord-279463-bli8hwda	95	21	)	)	-RRB-
cord-279463-bli8hwda	95	22	of	of	IN
cord-279463-bli8hwda	95	23	RM	RM	NNP
cord-279463-bli8hwda	95	24	.	.	.
cord-279463-bli8hwda	96	1	The	the	DT
cord-279463-bli8hwda	96	2	membrane	membrane	NN
cord-279463-bli8hwda	96	3	topology	topology	NN
cord-279463-bli8hwda	96	4	of	of	IN
cord-279463-bli8hwda	96	5	ly	ly	NNP
cord-279463-bli8hwda	96	6	was	be	VBD
cord-279463-bli8hwda	96	7	determined	determine	VBN
cord-279463-bli8hwda	96	8	by	by	IN
cord-279463-bli8hwda	96	9	treatment	treatment	NN
cord-279463-bli8hwda	96	10	with	with	IN
cord-279463-bli8hwda	96	11	proteinase	proteinase	NN
cord-279463-bli8hwda	96	12	K	K	NNP
cord-279463-bli8hwda	96	13	(	(	-LRB-
cord-279463-bli8hwda	96	14	PK	PK	NNP
cord-279463-bli8hwda	96	15	)	)	-RRB-
cord-279463-bli8hwda	96	16	(	(	-LRB-
cord-279463-bli8hwda	96	17	lanes	lane	NNS
cord-279463-bli8hwda	96	18	3	3	CD
cord-279463-bli8hwda	96	19	,	,	,
cord-279463-bli8hwda	96	20	7	7	CD
cord-279463-bli8hwda	96	21	,	,	,
cord-279463-bli8hwda	96	22	and	and	CC
cord-279463-bli8hwda	96	23	10	10	CD
cord-279463-bli8hwda	96	24	)	)	-RRB-
cord-279463-bli8hwda	96	25	or	or	CC
cord-279463-bli8hwda	96	26	PK	PK	NNP
cord-279463-bli8hwda	96	27	and	and	CC
cord-279463-bli8hwda	96	28	the	the	DT
cord-279463-bli8hwda	96	29	detergent	detergent	NN
cord-279463-bli8hwda	97	1	NP40	NP40	NNP
cord-279463-bli8hwda	97	2	(	(	-LRB-
cord-279463-bli8hwda	97	3	lane	lane	NN
cord-279463-bli8hwda	97	4	4	4	CD
cord-279463-bli8hwda	97	5	)	)	-RRB-
cord-279463-bli8hwda	97	6	.	.	.
cord-279463-bli8hwda	98	1	Proteins	protein	NNS
cord-279463-bli8hwda	98	2	were	be	VBD
cord-279463-bli8hwda	98	3	separated	separate	VBN
cord-279463-bli8hwda	98	4	by	by	IN
cord-279463-bli8hwda	98	5	SDS	SDS	NNP
cord-279463-bli8hwda	98	6	-	-	HYPH
cord-279463-bli8hwda	98	7	PAGE	PAGE	NNP
cord-279463-bli8hwda	98	8	and	and	CC
cord-279463-bli8hwda	98	9	visualized	visualize	VBN
cord-279463-bli8hwda	98	10	by	by	IN
cord-279463-bli8hwda	98	11	autoradiography	autoradiography	NNP
cord-279463-bli8hwda	98	12	.	.	.
cord-279463-bli8hwda	99	1	Lanes	lane	NNS
cord-279463-bli8hwda	99	2	1	1	CD
cord-279463-bli8hwda	99	3	-	-	SYM
cord-279463-bli8hwda	99	4	4	4	CD
cord-279463-bli8hwda	99	5	show	show	VBP
cord-279463-bli8hwda	99	6	total	total	JJ
cord-279463-bli8hwda	99	7	protein	protein	NN
cord-279463-bli8hwda	99	8	synthesized	synthesize	VBN
cord-279463-bli8hwda	99	9	.	.	.
cord-279463-bli8hwda	100	1	Samples	sample	NNS
cord-279463-bli8hwda	100	2	characterized	characterize	VBN
cord-279463-bli8hwda	100	3	in	in	IN
cord-279463-bli8hwda	100	4	lanes	lane	NNS
cord-279463-bli8hwda	100	5	5	5	CD
cord-279463-bli8hwda	100	6	-	-	SYM
cord-279463-bli8hwda	100	7	7	7	CD
cord-279463-bli8hwda	100	8	were	be	VBD
cord-279463-bli8hwda	100	9	immunoprecipitated	immunoprecipitate	VBN
cord-279463-bli8hwda	100	10	with	with	IN
cord-279463-bli8hwda	100	11	an	an	DT
cord-279463-bli8hwda	100	12	antibody	antibody	NN
cord-279463-bli8hwda	100	13	raised	raise	VBN
cord-279463-bli8hwda	100	14	against	against	IN
cord-279463-bli8hwda	100	15	the	the	DT
cord-279463-bli8hwda	100	16	amino	amino	NN
cord-279463-bli8hwda	100	17	-	-	HYPH
cord-279463-bli8hwda	100	18	terminal	terminal	NN
cord-279463-bli8hwda	100	19	72	72	CD
cord-279463-bli8hwda	100	20	amino	amino	NN
cord-279463-bli8hwda	100	21	acid	acid	NN
cord-279463-bli8hwda	100	22	residues	residue	NNS
cord-279463-bli8hwda	100	23	of	of	IN
cord-279463-bli8hwda	100	24	ly	ly	JJ
cord-279463-bli8hwda	100	25	(	(	-LRB-
cord-279463-bli8hwda	100	26	anti	anti	JJ
cord-279463-bli8hwda	100	27	-	-	JJ
cord-279463-bli8hwda	100	28	IyN	iyn	JJ
cord-279463-bli8hwda	100	29	)	)	-RRB-
cord-279463-bli8hwda	100	30	;	;	:
cord-279463-bli8hwda	100	31	in	in	IN
cord-279463-bli8hwda	100	32	lanes	lane	NNS
cord-279463-bli8hwda	100	33	8	8	CD
cord-279463-bli8hwda	100	34	-	-	SYM
cord-279463-bli8hwda	100	35	10	10	CD
cord-279463-bli8hwda	100	36	,	,	,
cord-279463-bli8hwda	100	37	with	with	IN
cord-279463-bli8hwda	100	38	an	an	DT
cord-279463-bli8hwda	100	39	antibody	antibody	NN
cord-279463-bli8hwda	100	40	against	against	IN
cord-279463-bli8hwda	100	41	the	the	DT
cord-279463-bli8hwda	100	42	carboxy	carboxy	JJ
cord-279463-bli8hwda	100	43	-	-	HYPH
cord-279463-bli8hwda	100	44	terminal	terminal	NN
cord-279463-bli8hwda	100	45	portion	portion	NN
cord-279463-bli8hwda	100	46	of	of	IN
cord-279463-bli8hwda	100	47	ly	ly	JJ
cord-279463-bli8hwda	100	48	(	(	-LRB-
cord-279463-bli8hwda	100	49	anti	anti	JJ
cord-279463-bli8hwda	100	50	-	-	JJ
cord-279463-bli8hwda	100	51	IyC	iyc	JJ
cord-279463-bli8hwda	100	52	)	)	-RRB-
cord-279463-bli8hwda	100	53	.	.	.
cord-279463-bli8hwda	101	1	The	the	DT
cord-279463-bli8hwda	101	2	increase	increase	NN
cord-279463-bli8hwda	101	3	in	in	IN
cord-279463-bli8hwda	101	4	molecular	molecular	JJ
cord-279463-bli8hwda	101	5	weight	weight	NN
cord-279463-bli8hwda	101	6	is	be	VBZ
cord-279463-bli8hwda	101	7	consistent	consistent	JJ
cord-279463-bli8hwda	101	8	with	with	IN
cord-279463-bli8hwda	101	9	the	the	DT
cord-279463-bli8hwda	101	10	addition	addition	NN
cord-279463-bli8hwda	101	11	of	of	IN
cord-279463-bli8hwda	101	12	one	one	CD
cord-279463-bli8hwda	101	13	N	n	CD
cord-279463-bli8hwda	101	14	-	-	HYPH
cord-279463-bli8hwda	101	15	linked	link	VBN
cord-279463-bli8hwda	101	16	oligosaccharide	oligosaccharide	NN
cord-279463-bli8hwda	101	17	to	to	IN
cord-279463-bli8hwda	101	18	the	the	DT
cord-279463-bli8hwda	101	19	CAT	cat	NN
cord-279463-bli8hwda	101	20	-	-	HYPH
cord-279463-bli8hwda	101	21	derived	derive	VBN
cord-279463-bli8hwda	101	22	portion	portion	NN
cord-279463-bli8hwda	101	23	.	.	.
cord-279463-bli8hwda	102	1	There	there	EX
cord-279463-bli8hwda	102	2	is	be	VBZ
cord-279463-bli8hwda	102	3	one	one	CD
cord-279463-bli8hwda	102	4	potential	potential	JJ
cord-279463-bli8hwda	102	5	site	site	NN
cord-279463-bli8hwda	102	6	for	for	IN
cord-279463-bli8hwda	102	7	N	n	CD
cord-279463-bli8hwda	102	8	-	-	HYPH
cord-279463-bli8hwda	102	9	linked	link	VBN
cord-279463-bli8hwda	102	10	glycosylation	glycosylation	NN
cord-279463-bli8hwda	102	11	in	in	IN
cord-279463-bli8hwda	102	12	the	the	DT
cord-279463-bli8hwda	102	13	CAT	cat	NN
cord-279463-bli8hwda	102	14	protein	protein	NN
cord-279463-bli8hwda	102	15	.	.	.
cord-279463-bli8hwda	103	1	After	after	IN
cord-279463-bli8hwda	103	2	protease	protease	JJ
cord-279463-bli8hwda	103	3	digestion	digestion	NN
cord-279463-bli8hwda	103	4	in	in	IN
cord-279463-bli8hwda	103	5	the	the	DT
cord-279463-bli8hwda	103	6	presence	presence	NN
cord-279463-bli8hwda	103	7	of	of	IN
cord-279463-bli8hwda	103	8	microsomal	microsomal	NNP
cord-279463-bli8hwda	103	9	membranes	membrane	NNS
cord-279463-bli8hwda	103	10	,	,	,
cord-279463-bli8hwda	103	11	lyCAT	lycat	FW
cord-279463-bli8hwda	103	12	*	*	NFP
cord-279463-bli8hwda	103	13	is	be	VBZ
cord-279463-bli8hwda	103	14	reduced	reduce	VBN
cord-279463-bli8hwda	103	15	in	in	IN
cord-279463-bli8hwda	103	16	molecular	molecular	JJ
cord-279463-bli8hwda	103	17	weight	weight	NN
cord-279463-bli8hwda	103	18	by	by	IN
cord-279463-bli8hwda	103	19	about	about	RB
cord-279463-bli8hwda	103	20	2	2	CD
cord-279463-bli8hwda	103	21	kd	kd	NNS
cord-279463-bli8hwda	103	22	,	,	,
cord-279463-bli8hwda	103	23	suggesting	suggest	VBG
cord-279463-bli8hwda	103	24	that	that	IN
cord-279463-bli8hwda	103	25	it	-PRON-	PRP
cord-279463-bli8hwda	103	26	exposes	expose	VBZ
cord-279463-bli8hwda	103	27	20	20	CD
cord-279463-bli8hwda	103	28	-	-	SYM
cord-279463-bli8hwda	103	29	30	30	CD
cord-279463-bli8hwda	103	30	amino	amino	NN
cord-279463-bli8hwda	103	31	acid	acid	NN
cord-279463-bli8hwda	103	32	residues	residue	NNS
cord-279463-bli8hwda	103	33	on	on	IN
cord-279463-bli8hwda	103	34	the	the	DT
cord-279463-bli8hwda	103	35	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	103	36	side	side	NN
cord-279463-bli8hwda	103	37	(	(	-LRB-
cord-279463-bli8hwda	103	38	Figure	figure	NN
cord-279463-bli8hwda	103	39	3	3	CD
cord-279463-bli8hwda	103	40	,	,	,
cord-279463-bli8hwda	103	41	lanes	lane	NNS
cord-279463-bli8hwda	103	42	2	2	CD
cord-279463-bli8hwda	103	43	and	and	CC
cord-279463-bli8hwda	103	44	3	3	CD
cord-279463-bli8hwda	103	45	)	)	-RRB-
cord-279463-bli8hwda	103	46	.	.	.
cord-279463-bli8hwda	104	1	CAT	cat	NN
cord-279463-bli8hwda	104	2	protein	protein	NN
cord-279463-bli8hwda	104	3	obtained	obtain	VBN
cord-279463-bli8hwda	104	4	after	after	IN
cord-279463-bli8hwda	104	5	transcription	transcription	NN
cord-279463-bli8hwda	104	6	-	-	HYPH
cord-279463-bli8hwda	104	7	translation	translation	NN
cord-279463-bli8hwda	104	8	from	from	IN
cord-279463-bli8hwda	104	9	pDS5	pDS5	NNP
cord-279463-bli8hwda	104	10	is	be	VBZ
cord-279463-bli8hwda	104	11	not	not	RB
cord-279463-bli8hwda	104	12	modified	modify	VBN
cord-279463-bli8hwda	104	13	by	by	IN
cord-279463-bli8hwda	104	14	the	the	DT
cord-279463-bli8hwda	104	15	added	add	VBN
cord-279463-bli8hwda	104	16	microsomes	microsome	NNS
cord-279463-bli8hwda	104	17	.	.	.
cord-279463-bli8hwda	105	1	As	as	IN
cord-279463-bli8hwda	105	2	expected	expect	VBN
cord-279463-bli8hwda	105	3	,	,	,
cord-279463-bli8hwda	105	4	no	no	DT
cord-279463-bli8hwda	105	5	shift	shift	NN
cord-279463-bli8hwda	105	6	in	in	IN
cord-279463-bli8hwda	105	7	molecular	molecular	JJ
cord-279463-bli8hwda	105	8	weight	weight	NN
cord-279463-bli8hwda	105	9	can	can	MD
cord-279463-bli8hwda	105	10	be	be	VB
cord-279463-bli8hwda	105	11	seen	see	VBN
cord-279463-bli8hwda	105	12	(	(	-LRB-
cord-279463-bli8hwda	105	13	Figure	figure	NN
cord-279463-bli8hwda	105	14	3	3	CD
cord-279463-bli8hwda	105	15	,	,	,
cord-279463-bli8hwda	105	16	lanes	lane	NNS
cord-279463-bli8hwda	105	17	5	5	CD
cord-279463-bli8hwda	105	18	and	and	CC
cord-279463-bli8hwda	105	19	6	6	CD
cord-279463-bli8hwda	105	20	)	)	-RRB-
cord-279463-bli8hwda	105	21	.	.	.
cord-279463-bli8hwda	106	1	CAT	cat	NN
cord-279463-bli8hwda	106	2	protein	protein	NN
cord-279463-bli8hwda	106	3	was	be	VBD
cord-279463-bli8hwda	106	4	very	very	RB
cord-279463-bli8hwda	106	5	resistant	resistant	JJ
cord-279463-bli8hwda	106	6	to	to	IN
cord-279463-bli8hwda	106	7	protease	protease	NN
cord-279463-bli8hwda	106	8	digestion	digestion	NN
cord-279463-bli8hwda	106	9	even	even	RB
cord-279463-bli8hwda	106	10	in	in	IN
cord-279463-bli8hwda	106	11	the	the	DT
cord-279463-bli8hwda	106	12	presence	presence	NN
cord-279463-bli8hwda	106	13	of	of	IN
cord-279463-bli8hwda	106	14	NP40	NP40	NNP
cord-279463-bli8hwda	107	1	(	(	-LRB-
cord-279463-bli8hwda	107	2	Figure	figure	NN
cord-279463-bli8hwda	107	3	3	3	CD
cord-279463-bli8hwda	107	4	,	,	,
cord-279463-bli8hwda	107	5	lanes	lane	NNS
cord-279463-bli8hwda	107	6	7	7	CD
cord-279463-bli8hwda	107	7	and	and	CC
cord-279463-bli8hwda	107	8	8)	8)	NN
cord-279463-bli8hwda	107	9	.	.	.
cord-279463-bli8hwda	108	1	I-&AT	I-&AT	NNP
cord-279463-bli8hwda	108	2	,	,	,
cord-279463-bli8hwda	108	3	in	in	IN
cord-279463-bli8hwda	108	4	contrast	contrast	NN
cord-279463-bli8hwda	108	5	,	,	,
cord-279463-bli8hwda	108	6	was	be	VBD
cord-279463-bli8hwda	108	7	very	very	RB
cord-279463-bli8hwda	108	8	sensitive	sensitive	JJ
cord-279463-bli8hwda	108	9	to	to	IN
cord-279463-bli8hwda	108	10	added	add	VBN
cord-279463-bli8hwda	108	11	protease	protease	NN
cord-279463-bli8hwda	108	12	.	.	.
cord-279463-bli8hwda	109	1	This	this	DT
cord-279463-bli8hwda	109	2	might	may	MD
cord-279463-bli8hwda	109	3	reflect	reflect	VB
cord-279463-bli8hwda	109	4	a	a	DT
cord-279463-bli8hwda	109	5	difference	difference	NN
cord-279463-bli8hwda	109	6	in	in	IN
cord-279463-bli8hwda	109	7	conformation	conformation	NN
cord-279463-bli8hwda	109	8	between	between	IN
cord-279463-bli8hwda	109	9	the	the	DT
cord-279463-bli8hwda	109	10	free	free	JJ
cord-279463-bli8hwda	109	11	CAT	cat	NN
cord-279463-bli8hwda	109	12	protein	protein	NN
cord-279463-bli8hwda	109	13	and	and	CC
cord-279463-bli8hwda	109	14	the	the	DT
cord-279463-bli8hwda	109	15	CAT	cat	NN
cord-279463-bli8hwda	109	16	-	-	HYPH
cord-279463-bli8hwda	109	17	derived	derive	VBN
cord-279463-bli8hwda	109	18	portion	portion	NN
cord-279463-bli8hwda	109	19	in	in	IN
cord-279463-bli8hwda	109	20	IyCAT	IyCAT	NNP
cord-279463-bli8hwda	109	21	.	.	.
cord-279463-bli8hwda	110	1	The	the	DT
cord-279463-bli8hwda	110	2	location	location	NN
cord-279463-bli8hwda	110	3	of	of	IN
cord-279463-bli8hwda	110	4	CAT	cat	NN
cord-279463-bli8hwda	110	5	outside	outside	IN
cord-279463-bli8hwda	110	6	of	of	IN
cord-279463-bli8hwda	110	7	the	the	DT
cord-279463-bli8hwda	110	8	membrane	membrane	NN
cord-279463-bli8hwda	110	9	vesicles	vesicle	NNS
cord-279463-bli8hwda	110	10	can	can	MD
cord-279463-bli8hwda	110	11	be	be	VB
cord-279463-bli8hwda	110	12	demonstrated	demonstrate	VBN
cord-279463-bli8hwda	110	13	by	by	IN
cord-279463-bli8hwda	110	14	sedimenting	sediment	VBG
cord-279463-bli8hwda	110	15	the	the	DT
cord-279463-bli8hwda	110	16	membranes	membrane	NNS
cord-279463-bli8hwda	110	17	by	by	IN
cord-279463-bli8hwda	110	18	centrifugation	centrifugation	NN
cord-279463-bli8hwda	110	19	.	.	.
cord-279463-bli8hwda	111	1	CAT	cat	NN
cord-279463-bli8hwda	111	2	protein	protein	NN
cord-279463-bli8hwda	111	3	is	be	VBZ
cord-279463-bli8hwda	111	4	then	then	RB
cord-279463-bli8hwda	111	5	found	find	VBN
cord-279463-bli8hwda	111	6	in	in	IN
cord-279463-bli8hwda	111	7	the	the	DT
cord-279463-bli8hwda	111	8	supernatant	supernatant	NN
cord-279463-bli8hwda	111	9	(	(	-LRB-
cord-279463-bli8hwda	111	10	data	datum	NNS
cord-279463-bli8hwda	111	11	not	not	RB
cord-279463-bli8hwda	111	12	shown	show	VBN
cord-279463-bli8hwda	111	13	)	)	-RRB-
cord-279463-bli8hwda	111	14	.	.	.
cord-279463-bli8hwda	112	1	We	-PRON-	PRP
cord-279463-bli8hwda	112	2	conclude	conclude	VBP
cord-279463-bli8hwda	112	3	from	from	IN
cord-279463-bli8hwda	112	4	the	the	DT
cord-279463-bli8hwda	112	5	data	datum	NNS
cord-279463-bli8hwda	112	6	obtained	obtain	VBN
cord-279463-bli8hwda	112	7	with	with	IN
cord-279463-bli8hwda	112	8	IrCAT	IrCAT	NNP
cord-279463-bli8hwda	112	9	and	and	CC
cord-279463-bli8hwda	112	10	CAT	cat	VB
cord-279463-bli8hwda	112	11	that	that	WDT
cord-279463-bli8hwda	112	12	the	the	DT
cord-279463-bli8hwda	112	13	signal	signal	NN
cord-279463-bli8hwda	112	14	for	for	IN
cord-279463-bli8hwda	112	15	membrane	membrane	NN
cord-279463-bli8hwda	112	16	insertion	insertion	NN
cord-279463-bli8hwda	112	17	must	must	MD
cord-279463-bli8hwda	112	18	be	be	VB
cord-279463-bli8hwda	112	19	located	locate	VBN
cord-279463-bli8hwda	112	20	within	within	IN
cord-279463-bli8hwda	112	21	the	the	DT
cord-279463-bli8hwda	112	22	first	first	JJ
cord-279463-bli8hwda	112	23	72	72	CD
cord-279463-bli8hwda	112	24	amino	amino	NN
cord-279463-bli8hwda	112	25	acid	acid	NN
cord-279463-bli8hwda	112	26	residues	residue	NNS
cord-279463-bli8hwda	112	27	of	of	IN
cord-279463-bli8hwda	112	28	ly	ly	XX
cord-279463-bli8hwda	112	29	.	.	.
cord-279463-bli8hwda	113	1	To	to	TO
cord-279463-bli8hwda	113	2	localize	localize	VB
cord-279463-bli8hwda	113	3	this	this	DT
cord-279463-bli8hwda	113	4	signal	signal	NN
cord-279463-bli8hwda	113	5	more	more	RBR
cord-279463-bli8hwda	113	6	precisely	precisely	RB
cord-279463-bli8hwda	113	7	,	,	,
cord-279463-bli8hwda	113	8	we	-PRON-	PRP
cord-279463-bli8hwda	113	9	deleted	delete	VBD
cord-279463-bli8hwda	113	10	the	the	DT
cord-279463-bli8hwda	113	11	first	first	JJ
cord-279463-bli8hwda	113	12	30	30	CD
cord-279463-bli8hwda	113	13	residues	residue	NNS
cord-279463-bli8hwda	113	14	of	of	IN
cord-279463-bli8hwda	113	15	IyCAT	IyCAT	NNP
cord-279463-bli8hwda	113	16	.	.	.
cord-279463-bli8hwda	114	1	CAT-	CAT-	NNP
cord-279463-bli8hwda	115	1	Insertion	insertion	NN
cord-279463-bli8hwda	115	2	of	of	IN
cord-279463-bli8hwda	115	3	k&AT	k&AT	NNP
cord-279463-bli8hwda	115	4	and	and	CC
cord-279463-bli8hwda	115	5	CAT	CAT	NNP
cord-279463-bli8hwda	115	6	Protein	Protein	NNP
cord-279463-bli8hwda	115	7	RNA	RNA	NNP
cord-279463-bli8hwda	115	8	derived	derive	VBD
cord-279463-bli8hwda	115	9	from	from	IN
cord-279463-bli8hwda	115	10	plycat	plycat	NN
cord-279463-bli8hwda	115	11	or	or	CC
cord-279463-bli8hwda	115	12	pDS5	pDS5	NNP
cord-279463-bli8hwda	115	13	was	be	VBD
cord-279463-bli8hwda	115	14	translated	translate	VBN
cord-279463-bli8hwda	115	15	in	in	IN
cord-279463-bli8hwda	115	16	the	the	DT
cord-279463-bli8hwda	115	17	wheat	wheat	NN
cord-279463-bli8hwda	115	18	germ	germ	NN
cord-279463-bli8hwda	115	19	cell	cell	NN
cord-279463-bli8hwda	115	20	-	-	HYPH
cord-279463-bli8hwda	115	21	free	free	JJ
cord-279463-bli8hwda	115	22	system	system	NN
cord-279463-bli8hwda	115	23	in	in	IN
cord-279463-bli8hwda	115	24	the	the	DT
cord-279463-bli8hwda	115	25	absence	absence	NN
cord-279463-bli8hwda	115	26	or	or	CC
cord-279463-bli8hwda	115	27	the	the	DT
cord-279463-bli8hwda	115	28	presence	presence	NN
cord-279463-bli8hwda	115	29	of	of	IN
cord-279463-bli8hwda	115	30	RM	RM	NNP
cord-279463-bli8hwda	115	31	.	.	.
cord-279463-bli8hwda	116	1	Membrane	membrane	NN
cord-279463-bli8hwda	116	2	insertion	insertion	NN
cord-279463-bli8hwda	116	3	was	be	VBD
cord-279463-bli8hwda	116	4	tested	test	VBN
cord-279463-bli8hwda	116	5	by	by	IN
cord-279463-bli8hwda	116	6	treatment	treatment	NN
cord-279463-bli8hwda	116	7	with	with	IN
cord-279463-bli8hwda	116	8	proteinase	proteinase	NN
cord-279463-bli8hwda	116	9	K	K	NNP
cord-279463-bli8hwda	116	10	(	(	-LRB-
cord-279463-bli8hwda	116	11	PK	PK	NNP
cord-279463-bli8hwda	116	12	)	)	-RRB-
cord-279463-bli8hwda	116	13	and	and	CC
cord-279463-bli8hwda	116	14	NP40	NP40	NNP
cord-279463-bli8hwda	116	15	.	.	.
cord-279463-bli8hwda	117	1	Addition	addition	NN
cord-279463-bli8hwda	117	2	of	of	IN
cord-279463-bli8hwda	117	3	RM	RM	NNP
cord-279463-bli8hwda	117	4	,	,	,
cord-279463-bli8hwda	117	5	PK	PK	NNP
cord-279463-bli8hwda	117	6	,	,	,
cord-279463-bli8hwda	117	7	and	and	CC
cord-279463-bli8hwda	118	1	NP40	NP40	NNP
cord-279463-bli8hwda	118	2	is	be	VBZ
cord-279463-bli8hwda	118	3	indicated	indicate	VBN
cord-279463-bli8hwda	118	4	at	at	IN
cord-279463-bli8hwda	118	5	the	the	DT
cord-279463-bli8hwda	118	6	bottom	bottom	NN
cord-279463-bli8hwda	118	7	of	of	IN
cord-279463-bli8hwda	118	8	each	each	DT
cord-279463-bli8hwda	118	9	lane	lane	NN
cord-279463-bli8hwda	118	10	.	.	.
cord-279463-bli8hwda	119	1	of	of	IN
cord-279463-bli8hwda	119	2	AN+CAT	AN+CAT	NNP
cord-279463-bli8hwda	120	1	In	in	IN
cord-279463-bli8hwda	120	2	all	all	DT
cord-279463-bli8hwda	120	3	secretory	secretory	JJ
cord-279463-bli8hwda	120	4	proteins	protein	NNS
cord-279463-bli8hwda	120	5	the	the	DT
cord-279463-bli8hwda	120	6	cleavable	cleavable	JJ
cord-279463-bli8hwda	120	7	signal	signal	NN
cord-279463-bli8hwda	120	8	for	for	IN
cord-279463-bli8hwda	120	9	membrane	membrane	NN
cord-279463-bli8hwda	120	10	translocation	translocation	NN
cord-279463-bli8hwda	120	11	is	be	VBZ
cord-279463-bli8hwda	120	12	located	locate	VBN
cord-279463-bli8hwda	120	13	at	at	IN
cord-279463-bli8hwda	120	14	the	the	DT
cord-279463-bli8hwda	120	15	amino	amino	NN
cord-279463-bli8hwda	120	16	-	-	HYPH
cord-279463-bli8hwda	120	17	terminal	terminal	NN
cord-279463-bli8hwda	120	18	end	end	NN
cord-279463-bli8hwda	120	19	of	of	IN
cord-279463-bli8hwda	120	20	the	the	DT
cord-279463-bli8hwda	120	21	precursor	precursor	NN
cord-279463-bli8hwda	120	22	polypeptide	polypeptide	NN
cord-279463-bli8hwda	120	23	.	.	.
cord-279463-bli8hwda	121	1	The	the	DT
cord-279463-bli8hwda	121	2	main	main	JJ
cord-279463-bli8hwda	121	3	feature	feature	NN
cord-279463-bli8hwda	121	4	of	of	IN
cord-279463-bli8hwda	121	5	this	this	DT
cord-279463-bli8hwda	121	6	signal	signal	NN
cord-279463-bli8hwda	121	7	appears	appear	VBZ
cord-279463-bli8hwda	121	8	to	to	TO
cord-279463-bli8hwda	121	9	be	be	VB
cord-279463-bli8hwda	121	10	its	-PRON-	PRP$
cord-279463-bli8hwda	121	11	hydrophobicity	hydrophobicity	NN
cord-279463-bli8hwda	121	12	.	.	.
cord-279463-bli8hwda	122	1	In	in	IN
cord-279463-bli8hwda	122	2	ly	ly	IN
cord-279463-bli8hwda	122	3	the	the	DT
cord-279463-bli8hwda	122	4	only	only	JJ
cord-279463-bli8hwda	122	5	hydrophobic	hydrophobic	JJ
cord-279463-bli8hwda	122	6	stretch	stretch	NN
cord-279463-bli8hwda	122	7	of	of	IN
cord-279463-bli8hwda	122	8	amino	amino	JJ
cord-279463-bli8hwda	122	9	acid	acid	NN
cord-279463-bli8hwda	122	10	residues	residue	NNS
cord-279463-bli8hwda	122	11	that	that	WDT
cord-279463-bli8hwda	122	12	resembles	resemble	VBZ
cord-279463-bli8hwda	122	13	a	a	DT
cord-279463-bli8hwda	122	14	signal	signal	JJ
cord-279463-bli8hwda	122	15	sequence	sequence	NN
cord-279463-bli8hwda	122	16	is	be	VBZ
cord-279463-bli8hwda	122	17	located	locate	VBN
cord-279463-bli8hwda	122	18	in	in	IN
cord-279463-bli8hwda	122	19	the	the	DT
cord-279463-bli8hwda	122	20	membrane	membrane	NN
cord-279463-bli8hwda	122	21	spanning	span	VBG
cord-279463-bli8hwda	122	22	region	region	NN
cord-279463-bli8hwda	122	23	about	about	RB
cord-279463-bli8hwda	122	24	30	30	CD
cord-279463-bli8hwda	122	25	amino	amino	NN
cord-279463-bli8hwda	122	26	acid	acid	NN
cord-279463-bli8hwda	122	27	residues	residue	NNS
cord-279463-bli8hwda	122	28	away	away	RB
cord-279463-bli8hwda	122	29	from	from	IN
cord-279463-bli8hwda	122	30	the	the	DT
cord-279463-bli8hwda	122	31	amino	amino	NN
cord-279463-bli8hwda	122	32	-	-	HYPH
cord-279463-bli8hwda	122	33	terminal	terminal	NN
cord-279463-bli8hwda	122	34	initiator	initiator	NN
cord-279463-bli8hwda	122	35	methionine	methionine	NN
cord-279463-bli8hwda	122	36	.	.	.
cord-279463-bli8hwda	123	1	We	-PRON-	PRP
cord-279463-bli8hwda	123	2	asked	ask	VBD
cord-279463-bli8hwda	123	3	whether	whether	IN
cord-279463-bli8hwda	123	4	removal	removal	NN
cord-279463-bli8hwda	123	5	of	of	IN
cord-279463-bli8hwda	123	6	the	the	DT
cord-279463-bli8hwda	123	7	30	30	CD
cord-279463-bli8hwda	123	8	amino	amino	NN
cord-279463-bli8hwda	123	9	-	-	HYPH
cord-279463-bli8hwda	123	10	terminal	terminal	NN
cord-279463-bli8hwda	123	11	residues	residue	NNS
cord-279463-bli8hwda	123	12	in	in	IN
cord-279463-bli8hwda	123	13	IyCAT	IyCAT	NNP
cord-279463-bli8hwda	123	14	would	would	MD
cord-279463-bli8hwda	123	15	affect	affect	VB
cord-279463-bli8hwda	123	16	its	-PRON-	PRP$
cord-279463-bli8hwda	123	17	membrane	membrane	NN
cord-279463-bli8hwda	123	18	insertion	insertion	NN
cord-279463-bli8hwda	123	19	and	and	CC
cord-279463-bli8hwda	123	20	topology	topology	NN
cord-279463-bli8hwda	123	21	.	.	.
cord-279463-bli8hwda	124	1	The	the	DT
cord-279463-bli8hwda	124	2	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	124	3	domain	domain	NN
cord-279463-bli8hwda	124	4	of	of	IN
cord-279463-bli8hwda	124	5	IyCAT	IyCAT	NNP
cord-279463-bli8hwda	124	6	was	be	VBD
cord-279463-bli8hwda	124	7	deleted	delete	VBN
cord-279463-bli8hwda	124	8	and	and	CC
cord-279463-bli8hwda	124	9	the	the	DT
cord-279463-bli8hwda	124	10	initiator	initiator	NN
cord-279463-bli8hwda	124	11	methionine	methionine	NN
cord-279463-bli8hwda	124	12	was	be	VBD
cord-279463-bli8hwda	124	13	placed	place	VBN
cord-279463-bli8hwda	124	14	in	in	IN
cord-279463-bli8hwda	124	15	front	front	NN
cord-279463-bli8hwda	124	16	of	of	IN
cord-279463-bli8hwda	124	17	the	the	DT
cord-279463-bli8hwda	124	18	membranespanning	membranespanne	VBG
cord-279463-bli8hwda	124	19	segment	segment	NN
cord-279463-bli8hwda	124	20	.	.	.
cord-279463-bli8hwda	125	1	The	the	DT
cord-279463-bli8hwda	125	2	amino	amino	NN
cord-279463-bli8hwda	125	3	-	-	HYPH
cord-279463-bli8hwda	125	4	terminal	terminal	NN
cord-279463-bli8hwda	125	5	sequences	sequence	NNS
cord-279463-bli8hwda	125	6	of	of	IN
cord-279463-bli8hwda	125	7	ly	ly	JJ
cord-279463-bli8hwda	125	8	-	-	NN
cord-279463-bli8hwda	125	9	CAT	cat	NN
cord-279463-bli8hwda	125	10	and	and	CC
cord-279463-bli8hwda	125	11	AN	AN	NNP
cord-279463-bli8hwda	125	12	-	-	:
cord-279463-bli8hwda	125	13	I$AT	I$AT	NNP
cord-279463-bli8hwda	125	14	as	as	RB
cord-279463-bli8hwda	125	15	deduced	deduce	VBN
cord-279463-bli8hwda	125	16	from	from	IN
cord-279463-bli8hwda	125	17	the	the	DT
cord-279463-bli8hwda	125	18	DNA	dna	NN
cord-279463-bli8hwda	125	19	sequences	sequence	NNS
cord-279463-bli8hwda	125	20	are	be	VBP
cord-279463-bli8hwda	125	21	shown	show	VBN
cord-279463-bli8hwda	125	22	in	in	IN
cord-279463-bli8hwda	125	23	Figure	figure	NN
cord-279463-bli8hwda	125	24	4A	4a	CD
cord-279463-bli8hwda	125	25	.	.	.
cord-279463-bli8hwda	126	1	When	when	WRB
cord-279463-bli8hwda	126	2	RNA	RNA	NNP
cord-279463-bli8hwda	126	3	derived	derive	VBN
cord-279463-bli8hwda	126	4	from	from	IN
cord-279463-bli8hwda	126	5	PANlycat	PANlycat	NNP
cord-279463-bli8hwda	126	6	was	be	VBD
cord-279463-bli8hwda	126	7	translated	translate	VBN
cord-279463-bli8hwda	126	8	in	in	IN
cord-279463-bli8hwda	126	9	the	the	DT
cord-279463-bli8hwda	126	10	wheat	wheat	NN
cord-279463-bli8hwda	126	11	germ	germ	NN
cord-279463-bli8hwda	126	12	cell	cell	NN
cord-279463-bli8hwda	126	13	-	-	HYPH
cord-279463-bli8hwda	126	14	free	free	JJ
cord-279463-bli8hwda	126	15	system	system	NN
cord-279463-bli8hwda	126	16	,	,	,
cord-279463-bli8hwda	126	17	a	a	DT
cord-279463-bli8hwda	126	18	single	single	JJ
cord-279463-bli8hwda	126	19	polypeptide	polypeptide	NN
cord-279463-bli8hwda	126	20	of	of	IN
cord-279463-bli8hwda	126	21	29	29	CD
cord-279463-bli8hwda	126	22	kd	kd	NNS
cord-279463-bli8hwda	126	23	was	be	VBD
cord-279463-bli8hwda	126	24	synthesized	synthesize	VBN
cord-279463-bli8hwda	126	25	,	,	,
cord-279463-bli8hwda	126	26	AN	AN	NNP
cord-279463-bli8hwda	126	27	-	-	HYPH
cord-279463-bli8hwda	126	28	IyCAT	IyCAT	NNP
cord-279463-bli8hwda	126	29	(	(	-LRB-
cord-279463-bli8hwda	126	30	Figure	Figure	NNP
cord-279463-bli8hwda	126	31	46	46	CD
cord-279463-bli8hwda	126	32	,	,	,
cord-279463-bli8hwda	126	33	lane	lane	NN
cord-279463-bli8hwda	126	34	1	1	CD
cord-279463-bli8hwda	126	35	)	)	-RRB-
cord-279463-bli8hwda	126	36	.	.	.
cord-279463-bli8hwda	127	1	This	this	DT
cord-279463-bli8hwda	127	2	was	be	VBD
cord-279463-bli8hwda	127	3	,	,	,
cord-279463-bli8hwda	127	4	as	as	IN
cord-279463-bli8hwda	127	5	expected	expect	VBN
cord-279463-bli8hwda	127	6	,	,	,
cord-279463-bli8hwda	127	7	about	about	RB
cord-279463-bli8hwda	127	8	3	3	CD
cord-279463-bli8hwda	127	9	kd	kd	NNP
cord-279463-bli8hwda	127	10	smaller	small	JJR
cord-279463-bli8hwda	127	11	than	than	IN
cord-279463-bli8hwda	127	12	the	the	DT
cord-279463-bli8hwda	127	13	IyCAT	IyCAT	NNP
cord-279463-bli8hwda	127	14	protein	protein	NN
cord-279463-bli8hwda	127	15	(	(	-LRB-
cord-279463-bli8hwda	127	16	Figure	Figure	NNP
cord-279463-bli8hwda	127	17	46	46	CD
cord-279463-bli8hwda	127	18	,	,	,
cord-279463-bli8hwda	127	19	lane	lane	NN
cord-279463-bli8hwda	127	20	1	1	CD
cord-279463-bli8hwda	127	21	)	)	-RRB-
cord-279463-bli8hwda	127	22	.	.	.
cord-279463-bli8hwda	128	1	In	in	IN
cord-279463-bli8hwda	128	2	the	the	DT
cord-279463-bli8hwda	128	3	presence	presence	NN
cord-279463-bli8hwda	128	4	of	of	IN
cord-279463-bli8hwda	128	5	microsomes	microsomes	NNP
cord-279463-bli8hwda	128	6	,	,	,
cord-279463-bli8hwda	128	7	two	two	CD
cord-279463-bli8hwda	128	8	new	new	JJ
cord-279463-bli8hwda	128	9	protein	protein	NN
cord-279463-bli8hwda	128	10	bands	band	NNS
cord-279463-bli8hwda	128	11	appeared	appear	VBD
cord-279463-bli8hwda	128	12	,	,	,
cord-279463-bli8hwda	128	13	one	one	CD
cord-279463-bli8hwda	128	14	about	about	IN
cord-279463-bli8hwda	128	15	1	1	CD
cord-279463-bli8hwda	128	16	kd	kd	NNP
cord-279463-bli8hwda	128	17	smaller	small	JJR
cord-279463-bli8hwda	128	18	and	and	CC
cord-279463-bli8hwda	128	19	one	one	CD
cord-279463-bli8hwda	128	20	2	2	CD
cord-279463-bli8hwda	128	21	kd	kd	NNP
cord-279463-bli8hwda	128	22	larger	large	JJR
cord-279463-bli8hwda	128	23	than	than	IN
cord-279463-bli8hwda	128	24	AN	an	DT
cord-279463-bli8hwda	129	1	-IrCAT	-ircat	CD
cord-279463-bli8hwda	130	1	Both	both	DT
cord-279463-bli8hwda	130	2	of	of	IN
cord-279463-bli8hwda	130	3	these	these	DT
cord-279463-bli8hwda	130	4	forms	form	NNS
cord-279463-bli8hwda	130	5	were	be	VBD
cord-279463-bli8hwda	130	6	resistant	resistant	JJ
cord-279463-bli8hwda	130	7	to	to	IN
cord-279463-bli8hwda	130	8	proteinase	proteinase	NN
cord-279463-bli8hwda	130	9	K	K	NNP
cord-279463-bli8hwda	130	10	,	,	,
cord-279463-bli8hwda	130	11	indicating	indicate	VBG
cord-279463-bli8hwda	130	12	that	that	IN
cord-279463-bli8hwda	130	13	they	-PRON-	PRP
cord-279463-bli8hwda	130	14	were	be	VBD
cord-279463-bli8hwda	130	15	inserted	insert	VBN
cord-279463-bli8hwda	130	16	into	into	IN
cord-279463-bli8hwda	130	17	or	or	CC
cord-279463-bli8hwda	130	18	translocated	translocate	VBN
cord-279463-bli8hwda	130	19	across	across	IN
cord-279463-bli8hwda	130	20	microsomal	microsomal	JJ
cord-279463-bli8hwda	130	21	membranes	membrane	NNS
cord-279463-bli8hwda	130	22	(	(	-LRB-
cord-279463-bli8hwda	130	23	Figure	Figure	NNP
cord-279463-bli8hwda	130	24	48	48	CD
cord-279463-bli8hwda	130	25	,	,	,
cord-279463-bli8hwda	130	26	lanes	lane	NNS
cord-279463-bli8hwda	130	27	3	3	CD
cord-279463-bli8hwda	130	28	and	and	CC
cord-279463-bli8hwda	130	29	4	4	CD
cord-279463-bli8hwda	130	30	)	)	-RRB-
cord-279463-bli8hwda	130	31	.	.	.
cord-279463-bli8hwda	131	1	We	-PRON-	PRP
cord-279463-bli8hwda	131	2	suspected	suspect	VBD
cord-279463-bli8hwda	131	3	that	that	IN
cord-279463-bli8hwda	131	4	the	the	DT
cord-279463-bli8hwda	131	5	smaller	small	JJR
cord-279463-bli8hwda	131	6	molecular	molecular	JJ
cord-279463-bli8hwda	131	7	weight	weight	NN
cord-279463-bli8hwda	131	8	form	form	NN
cord-279463-bli8hwda	131	9	was	be	VBD
cord-279463-bli8hwda	131	10	generated	generate	VBN
cord-279463-bli8hwda	131	11	by	by	IN
cord-279463-bli8hwda	131	12	signal	signal	NN
cord-279463-bli8hwda	131	13	peptidase	peptidase	NN
cord-279463-bli8hwda	131	14	cleavage	cleavage	NN
cord-279463-bli8hwda	131	15	without	without	IN
cord-279463-bli8hwda	131	16	concomitant	concomitant	JJ
cord-279463-bli8hwda	131	17	glycosylation	glycosylation	NN
cord-279463-bli8hwda	131	18	and	and	CC
cord-279463-bli8hwda	131	19	that	that	IN
cord-279463-bli8hwda	131	20	the	the	DT
cord-279463-bli8hwda	131	21	larger	large	JJR
cord-279463-bli8hwda	131	22	molecular	molecular	JJ
cord-279463-bli8hwda	131	23	weight	weight	NN
cord-279463-bli8hwda	131	24	form	form	NN
cord-279463-bli8hwda	131	25	was	be	VBD
cord-279463-bli8hwda	131	26	glycosylated	glycosylate	VBN
cord-279463-bli8hwda	131	27	and	and	CC
cord-279463-bli8hwda	131	28	cleaved	cleave	VBN
cord-279463-bli8hwda	131	29	by	by	IN
cord-279463-bli8hwda	131	30	signal	signal	NN
cord-279463-bli8hwda	131	31	peptidase	peptidase	NN
cord-279463-bli8hwda	131	32	.	.	.
cord-279463-bli8hwda	132	1	These	these	DT
cord-279463-bli8hwda	132	2	possibilities	possibility	NNS
cord-279463-bli8hwda	132	3	were	be	VBD
cord-279463-bli8hwda	132	4	tested	test	VBN
cord-279463-bli8hwda	132	5	.	.	.
cord-279463-bli8hwda	133	1	from	from	IN
cord-279463-bli8hwda	133	2	PAN	PAN	NNP
cord-279463-bli8hwda	133	3	-	-	HYPH
cord-279463-bli8hwda	133	4	lycat	lycat	NNP
cord-279463-bli8hwda	133	5	was	be	VBD
cord-279463-bli8hwda	133	6	translated	translate	VBN
cord-279463-bli8hwda	133	7	in	in	IN
cord-279463-bli8hwda	133	8	the	the	DT
cord-279463-bli8hwda	133	9	wheat	wheat	NN
cord-279463-bli8hwda	133	10	germ	germ	NN
cord-279463-bli8hwda	133	11	cell	cell	NN
cord-279463-bli8hwda	133	12	-	-	HYPH
cord-279463-bli8hwda	133	13	free	free	JJ
cord-279463-bli8hwda	133	14	system	system	NN
cord-279463-bli8hwda	133	15	in	in	IN
cord-279463-bli8hwda	133	16	the	the	DT
cord-279463-bli8hwda	133	17	absence	absence	NN
cord-279463-bli8hwda	133	18	or	or	CC
cord-279463-bli8hwda	133	19	presence	presence	NN
cord-279463-bli8hwda	133	20	of	of	IN
cord-279463-bli8hwda	133	21	RM	RM	NNP
cord-279463-bli8hwda	133	22	.	.	.
cord-279463-bli8hwda	134	1	Membrane	membrane	NN
cord-279463-bli8hwda	134	2	insertion	insertion	NN
cord-279463-bli8hwda	134	3	and	and	CC
cord-279463-bli8hwda	134	4	topology	topology	NN
cord-279463-bli8hwda	134	5	was	be	VBD
cord-279463-bli8hwda	134	6	tested	test	VBN
cord-279463-bli8hwda	134	7	by	by	IN
cord-279463-bli8hwda	134	8	treatment	treatment	NN
cord-279463-bli8hwda	134	9	with	with	IN
cord-279463-bli8hwda	134	10	proteinase	proteinase	NN
cord-279463-bli8hwda	134	11	K	K	NNP
cord-279463-bli8hwda	134	12	(	(	-LRB-
cord-279463-bli8hwda	134	13	PK	PK	NNP
cord-279463-bli8hwda	134	14	)	)	-RRB-
cord-279463-bli8hwda	134	15	and	and	CC
cord-279463-bli8hwda	135	1	NP40	NP40	NNP
cord-279463-bli8hwda	135	2	.	.	.
cord-279463-bli8hwda	136	1	Components	component	NNS
cord-279463-bli8hwda	136	2	were	be	VBD
cord-279463-bli8hwda	136	3	added	add	VBN
cord-279463-bli8hwda	136	4	as	as	IN
cord-279463-bli8hwda	136	5	indicated	indicate	VBN
cord-279463-bli8hwda	136	6	below	below	IN
cord-279463-bli8hwda	136	7	the	the	DT
cord-279463-bli8hwda	136	8	lanes	lane	NNS
cord-279463-bli8hwda	136	9	.	.	.
cord-279463-bli8hwda	137	1	IYCAT	IYCAT	NNP
cord-279463-bli8hwda	137	2	translated	translate	VBD
cord-279463-bli8hwda	137	3	in	in	IN
cord-279463-bli8hwda	137	4	the	the	DT
cord-279463-bli8hwda	137	5	wheat	wheat	NN
cord-279463-bli8hwda	137	6	germ	germ	NN
cord-279463-bli8hwda	137	7	cell	cell	NN
cord-279463-bli8hwda	137	8	-	-	HYPH
cord-279463-bli8hwda	137	9	free	free	JJ
cord-279463-bli8hwda	137	10	system	system	NN
cord-279463-bli8hwda	137	11	is	be	VBZ
cord-279463-bli8hwda	137	12	shown	show	VBN
cord-279463-bli8hwda	137	13	for	for	IN
cord-279463-bli8hwda	137	14	comparison	comparison	NN
cord-279463-bli8hwda	137	15	.	.	.
cord-279463-bli8hwda	138	1	Processed	process	VBN
cord-279463-bli8hwda	138	2	and	and	CC
cord-279463-bli8hwda	138	3	Glycoeylated	Glycoeylated	NNP
cord-279463-bli8hwda	139	1	To	to	TO
cord-279463-bli8hwda	139	2	detect	detect	VB
cord-279463-bli8hwda	139	3	the	the	DT
cord-279463-bli8hwda	139	4	signal	signal	NN
cord-279463-bli8hwda	139	5	peptide	peptide	NN
cord-279463-bli8hwda	139	6	cleavage	cleavage	NN
cord-279463-bli8hwda	139	7	of	of	IN
cord-279463-bli8hwda	139	8	a	a	DT
cord-279463-bli8hwda	139	9	glycosylated	glycosylated	JJ
cord-279463-bli8hwda	139	10	protein	protein	NN
cord-279463-bli8hwda	139	11	on	on	IN
cord-279463-bli8hwda	139	12	a	a	DT
cord-279463-bli8hwda	139	13	polyacrylamide	polyacrylamide	NN
cord-279463-bli8hwda	139	14	gel	gel	NN
cord-279463-bli8hwda	140	1	it	-PRON-	PRP
cord-279463-bli8hwda	140	2	is	be	VBZ
cord-279463-bli8hwda	140	3	necessary	necessary	JJ
cord-279463-bli8hwda	140	4	to	to	TO
cord-279463-bli8hwda	140	5	block	block	VB
cord-279463-bli8hwda	140	6	its	-PRON-	PRP$
cord-279463-bli8hwda	140	7	glycosylation	glycosylation	NN
cord-279463-bli8hwda	140	8	,	,	,
cord-279463-bli8hwda	140	9	but	but	CC
cord-279463-bli8hwda	140	10	still	still	RB
cord-279463-bli8hwda	140	11	allow	allow	VB
cord-279463-bli8hwda	140	12	membrane	membrane	NN
cord-279463-bli8hwda	140	13	insertion	insertion	NN
cord-279463-bli8hwda	140	14	to	to	TO
cord-279463-bli8hwda	140	15	occur	occur	VB
cord-279463-bli8hwda	140	16	.	.	.
cord-279463-bli8hwda	141	1	Addition	addition	NN
cord-279463-bli8hwda	141	2	of	of	IN
cord-279463-bli8hwda	141	3	N	n	NN
cord-279463-bli8hwda	141	4	-	-	HYPH
cord-279463-bli8hwda	141	5	linked	link	VBN
cord-279463-bli8hwda	141	6	oligosaccharides	oligosaccharide	NNS
cord-279463-bli8hwda	141	7	onto	onto	IN
cord-279463-bli8hwda	141	8	nascent	nascent	JJ
cord-279463-bli8hwda	141	9	polypeptides	polypeptide	NNS
cord-279463-bli8hwda	141	10	can	can	MD
cord-279463-bli8hwda	141	11	be	be	VB
cord-279463-bli8hwda	141	12	blocked	block	VBN
cord-279463-bli8hwda	141	13	by	by	IN
cord-279463-bli8hwda	141	14	including	include	VBG
cord-279463-bli8hwda	141	15	synthetic	synthetic	JJ
cord-279463-bli8hwda	141	16	acceptor	acceptor	NN
cord-279463-bli8hwda	141	17	peptides	peptide	NNS
cord-279463-bli8hwda	141	18	in	in	IN
cord-279463-bli8hwda	141	19	an	an	DT
cord-279463-bli8hwda	141	20	in	in	FW
cord-279463-bli8hwda	141	21	vitro	vitro	FW
cord-279463-bli8hwda	141	22	membrane	membrane	NN
cord-279463-bli8hwda	141	23	insertion	insertion	NN
cord-279463-bli8hwda	141	24	assay	assay	NN
cord-279463-bli8hwda	141	25	(	(	-LRB-
cord-279463-bli8hwda	141	26	Bause	Bause	NNP
cord-279463-bli8hwda	141	27	,	,	,
cord-279463-bli8hwda	141	28	1983	1983	CD
cord-279463-bli8hwda	141	29	;	;	:
cord-279463-bli8hwda	141	30	Lau	Lau	NNP
cord-279463-bli8hwda	141	31	et	et	NNP
cord-279463-bli8hwda	141	32	al	al	NNP
cord-279463-bli8hwda	141	33	.	.	NNP
cord-279463-bli8hwda	141	34	,	,	,
cord-279463-bli8hwda	141	35	1983	1983	CD
cord-279463-bli8hwda	141	36	)	)	-RRB-
cord-279463-bli8hwda	141	37	.	.	.
cord-279463-bli8hwda	142	1	IyCAT	IyCAT	NNP
cord-279463-bli8hwda	142	2	and	and	CC
cord-279463-bli8hwda	142	3	AN	AN	NNP
cord-279463-bli8hwda	142	4	-	-	:
cord-279463-bli8hwda	142	5	IyCAT	IyCAT	NNP
cord-279463-bli8hwda	142	6	were	be	VBD
cord-279463-bli8hwda	142	7	translated	translate	VBN
cord-279463-bli8hwda	142	8	in	in	IN
cord-279463-bli8hwda	142	9	the	the	DT
cord-279463-bli8hwda	142	10	presence	presence	NN
cord-279463-bli8hwda	142	11	of	of	IN
cord-279463-bli8hwda	142	12	microsomes	microsome	NNS
cord-279463-bli8hwda	142	13	with	with	IN
cord-279463-bli8hwda	142	14	and	and	CC
cord-279463-bli8hwda	142	15	without	without	IN
cord-279463-bli8hwda	142	16	the	the	DT
cord-279463-bli8hwda	142	17	acceptor	acceptor	NN
cord-279463-bli8hwda	142	18	peptide	peptide	NN
cord-279463-bli8hwda	142	19	Asn	Asn	NNP
cord-279463-bli8hwda	142	20	-	-	HYPH
cord-279463-bli8hwda	142	21	Leu	Leu	NNP
cord-279463-bli8hwda	142	22	-	-	HYPH
cord-279463-bli8hwda	142	23	Thr	Thr	NNP
cord-279463-bli8hwda	142	24	.	.	.
cord-279463-bli8hwda	143	1	The	the	DT
cord-279463-bli8hwda	143	2	size	size	NN
cord-279463-bli8hwda	143	3	of	of	IN
cord-279463-bli8hwda	143	4	IyCAT	IyCAT	NNP
cord-279463-bli8hwda	143	5	synthesized	synthesize	VBN
cord-279463-bli8hwda	143	6	in	in	IN
cord-279463-bli8hwda	143	7	the	the	DT
cord-279463-bli8hwda	143	8	presence	presence	NN
cord-279463-bli8hwda	143	9	of	of	IN
cord-279463-bli8hwda	143	10	RM	RM	NNP
cord-279463-bli8hwda	143	11	and	and	CC
cord-279463-bli8hwda	143	12	acceptor	acceptor	NN
cord-279463-bli8hwda	143	13	peptide	peptide	NN
cord-279463-bli8hwda	143	14	was	be	VBD
cord-279463-bli8hwda	143	15	indistinguishable	indistinguishable	JJ
cord-279463-bli8hwda	143	16	from	from	IN
cord-279463-bli8hwda	143	17	that	that	DT
cord-279463-bli8hwda	143	18	made	make	VBN
cord-279463-bli8hwda	143	19	in	in	IN
cord-279463-bli8hwda	143	20	the	the	DT
cord-279463-bli8hwda	143	21	absence	absence	NN
cord-279463-bli8hwda	143	22	of	of	IN
cord-279463-bli8hwda	143	23	RM	RM	NNP
cord-279463-bli8hwda	143	24	.	.	.
cord-279463-bli8hwda	144	1	When	when	WRB
cord-279463-bli8hwda	144	2	proteinase	proteinase	NN
cord-279463-bli8hwda	144	3	K	K	NNP
cord-279463-bli8hwda	144	4	was	be	VBD
cord-279463-bli8hwda	144	5	used	use	VBN
cord-279463-bli8hwda	144	6	to	to	TO
cord-279463-bli8hwda	144	7	digest	digest	VB
cord-279463-bli8hwda	144	8	its	-PRON-	PRP$
cord-279463-bli8hwda	144	9	cytoplasmically	cytoplasmically	NN
cord-279463-bli8hwda	144	10	exposed	expose	VBN
cord-279463-bli8hwda	144	11	domain	domain	NN
cord-279463-bli8hwda	144	12	,	,	,
cord-279463-bli8hwda	144	13	the	the	DT
cord-279463-bli8hwda	144	14	size	size	NN
cord-279463-bli8hwda	144	15	was	be	VBD
cord-279463-bli8hwda	144	16	reduced	reduce	VBN
cord-279463-bli8hwda	144	17	by	by	IN
cord-279463-bli8hwda	144	18	about	about	RB
cord-279463-bli8hwda	144	19	2	2	CD
cord-279463-bli8hwda	144	20	-	-	SYM
cord-279463-bli8hwda	144	21	3	3	CD
cord-279463-bli8hwda	144	22	kd	kd	NN
cord-279463-bli8hwda	144	23	(	(	-LRB-
cord-279463-bli8hwda	144	24	Figure	figure	NN
cord-279463-bli8hwda	144	25	5A	5a	NN
cord-279463-bli8hwda	144	26	)	)	-RRB-
cord-279463-bli8hwda	144	27	.	.	.
cord-279463-bli8hwda	145	1	We	-PRON-	PRP
cord-279463-bli8hwda	145	2	can	can	MD
cord-279463-bli8hwda	145	3	conclude	conclude	VB
cord-279463-bli8hwda	145	4	that	that	IN
cord-279463-bli8hwda	145	5	nonglycosylated	nonglycosylate	VBN
cord-279463-bli8hwda	145	6	IyCAT	IyCAT	NNP
cord-279463-bli8hwda	145	7	synthesized	synthesize	VBN
cord-279463-bli8hwda	145	8	in	in	IN
cord-279463-bli8hwda	145	9	the	the	DT
cord-279463-bli8hwda	145	10	presence	presence	NN
cord-279463-bli8hwda	145	11	of	of	IN
cord-279463-bli8hwda	145	12	RM	RM	NNP
cord-279463-bli8hwda	145	13	and	and	CC
cord-279463-bli8hwda	145	14	acceptor	acceptor	NN
cord-279463-bli8hwda	145	15	peptide	peptide	NN
cord-279463-bli8hwda	145	16	is	be	VBZ
cord-279463-bli8hwda	145	17	inserted	insert	VBN
cord-279463-bli8hwda	145	18	into	into	IN
cord-279463-bli8hwda	145	19	the	the	DT
cord-279463-bli8hwda	145	20	membrane	membrane	NN
cord-279463-bli8hwda	145	21	in	in	IN
cord-279463-bli8hwda	145	22	the	the	DT
cord-279463-bli8hwda	145	23	same	same	JJ
cord-279463-bli8hwda	145	24	way	way	NN
cord-279463-bli8hwda	145	25	as	as	IN
cord-279463-bli8hwda	145	26	its	-PRON-	PRP$
cord-279463-bli8hwda	145	27	glycosylated	glycosylated	JJ
cord-279463-bli8hwda	145	28	form	form	NN
cord-279463-bli8hwda	145	29	and	and	CC
cord-279463-bli8hwda	145	30	that	that	IN
cord-279463-bli8hwda	145	31	no	no	DT
cord-279463-bli8hwda	145	32	signal	signal	NN
cord-279463-bli8hwda	145	33	sequence	sequence	NN
cord-279463-bli8hwda	145	34	is	be	VBZ
cord-279463-bli8hwda	145	35	cleaved	cleave	VBN
cord-279463-bli8hwda	145	36	during	during	IN
cord-279463-bli8hwda	145	37	membrane	membrane	NN
cord-279463-bli8hwda	145	38	translocation	translocation	NN
cord-279463-bli8hwda	145	39	(	(	-LRB-
cord-279463-bli8hwda	145	40	Figure	figure	NN
cord-279463-bli8hwda	145	41	5A	5a	NN
cord-279463-bli8hwda	145	42	Figure	figure	NN
cord-279463-bli8hwda	145	43	58	58	CD
cord-279463-bli8hwda	145	44	,	,	,
cord-279463-bli8hwda	145	45	lanes	lane	NNS
cord-279463-bli8hwda	145	46	2	2	CD
cord-279463-bli8hwda	145	47	and	and	CC
cord-279463-bli8hwda	145	48	3	3	CD
cord-279463-bli8hwda	145	49	)	)	-RRB-
cord-279463-bli8hwda	145	50	.	.	.
cord-279463-bli8hwda	146	1	AN	an	LS
cord-279463-bli8hwda	146	2	-	-	:
cord-279463-bli8hwda	146	3	IyCAT'was	IyCAT'was	NNP
cord-279463-bli8hwda	146	4	also	also	RB
cord-279463-bli8hwda	146	5	found	find	VBD
cord-279463-bli8hwda	146	6	to	to	TO
cord-279463-bli8hwda	146	7	be	be	VB
cord-279463-bli8hwda	146	8	protected	protect	VBN
cord-279463-bli8hwda	146	9	against	against	IN
cord-279463-bli8hwda	146	10	exogenous	exogenous	JJ
cord-279463-bli8hwda	146	11	proteinase	proteinase	NN
cord-279463-bli8hwda	146	12	K	k	NN
cord-279463-bli8hwda	146	13	(	(	-LRB-
cord-279463-bli8hwda	146	14	Figure	Figure	NNP
cord-279463-bli8hwda	146	15	56	56	CD
cord-279463-bli8hwda	146	16	,	,	,
cord-279463-bli8hwda	146	17	lane	lane	NN
cord-279463-bli8hwda	146	18	4	4	CD
cord-279463-bli8hwda	146	19	)	)	-RRB-
cord-279463-bli8hwda	146	20	.	.	.
cord-279463-bli8hwda	147	1	This	this	DT
cord-279463-bli8hwda	147	2	suggested	suggest	VBD
cord-279463-bli8hwda	147	3	to	to	IN
cord-279463-bli8hwda	147	4	us	-PRON-	PRP
cord-279463-bli8hwda	147	5	that	that	IN
cord-279463-bli8hwda	147	6	the	the	DT
cord-279463-bli8hwda	147	7	larger	large	JJR
cord-279463-bli8hwda	147	8	form	form	NN
cord-279463-bli8hwda	147	9	was	be	VBD
cord-279463-bli8hwda	147	10	glycosylated	glycosylate	VBN
cord-279463-bli8hwda	147	11	and	and	CC
cord-279463-bli8hwda	147	12	proteolytically	proteolytically	RB
cord-279463-bli8hwda	147	13	processed	process	VBN
cord-279463-bli8hwda	147	14	and	and	CC
cord-279463-bli8hwda	147	15	that	that	IN
cord-279463-bli8hwda	147	16	AN	AN	NNP
cord-279463-bli8hwda	147	17	-	-	HYPH
cord-279463-bli8hwda	147	18	IyCAT	IyCAT	NNP
cord-279463-bli8hwda	147	19	'	'	''
cord-279463-bli8hwda	147	20	was	be	VBD
cord-279463-bli8hwda	147	21	generated	generate	VBN
cord-279463-bli8hwda	147	22	by	by	IN
cord-279463-bli8hwda	147	23	a	a	DT
cord-279463-bli8hwda	147	24	proteolytic	proteolytic	JJ
cord-279463-bli8hwda	147	25	cleavage	cleavage	NN
cord-279463-bli8hwda	147	26	,	,	,
cord-279463-bli8hwda	147	27	most	most	RBS
cord-279463-bli8hwda	147	28	likely	likely	JJ
cord-279463-bli8hwda	147	29	by	by	IN
cord-279463-bli8hwda	147	30	signal	signal	NN
cord-279463-bli8hwda	147	31	peptidase	peptidase	NN
cord-279463-bli8hwda	147	32	.	.	.
cord-279463-bli8hwda	148	1	To	to	TO
cord-279463-bli8hwda	148	2	determine	determine	VB
cord-279463-bli8hwda	148	3	the	the	DT
cord-279463-bli8hwda	148	4	site	site	NN
cord-279463-bli8hwda	148	5	of	of	IN
cord-279463-bli8hwda	148	6	cleavage	cleavage	NN
cord-279463-bli8hwda	148	7	in	in	IN
cord-279463-bli8hwda	148	8	the	the	DT
cord-279463-bli8hwda	148	9	proteolytically	proteolytically	RB
cord-279463-bli8hwda	148	10	processed	process	VBN
cord-279463-bli8hwda	148	11	forms	form	NNS
cord-279463-bli8hwda	148	12	of	of	IN
cord-279463-bli8hwda	148	13	AN	AN	NNP
cord-279463-bli8hwda	148	14	-	-	HYPH
cord-279463-bli8hwda	148	15	IrCAT	ircat	NN
cord-279463-bli8hwda	148	16	,	,	,
cord-279463-bli8hwda	148	17	the	the	DT
cord-279463-bli8hwda	148	18	positions	position	NNS
cord-279463-bli8hwda	148	19	of	of	IN
cord-279463-bli8hwda	148	20	leucine	leucine	NN
cord-279463-bli8hwda	148	21	in	in	IN
cord-279463-bli8hwda	148	22	the	the	DT
cord-279463-bli8hwda	148	23	amino	amino	NN
cord-279463-bli8hwda	148	24	-	-	HYPH
cord-279463-bli8hwda	148	25	terminal	terminal	NN
cord-279463-bli8hwda	148	26	regions	region	NNS
cord-279463-bli8hwda	148	27	of	of	IN
cord-279463-bli8hwda	148	28	AN	AN	NNP
cord-279463-bli8hwda	148	29	-	-	HYPH
cord-279463-bli8hwda	148	30	ITCAT	ITCAT	NNP
cord-279463-bli8hwda	148	31	and	and	CC
cord-279463-bli8hwda	148	32	membraneinserted	membraneinserte	VBD
cord-279463-bli8hwda	148	33	AN	AN	NNP
cord-279463-bli8hwda	148	34	-	-	HYPH
cord-279463-bli8hwda	148	35	lrCAT	lrcat	NN
cord-279463-bli8hwda	149	1	*	*	NFP
cord-279463-bli8hwda	149	2	'	'	''
cord-279463-bli8hwda	149	3	were	be	VBD
cord-279463-bli8hwda	149	4	determined	determine	VBN
cord-279463-bli8hwda	149	5	.	.	.
cord-279463-bli8hwda	150	1	AN	an	LS
cord-279463-bli8hwda	150	2	-	-	:
cord-279463-bli8hwda	150	3	I$AT	I$AT	NNP
cord-279463-bli8hwda	150	4	was	be	VBD
cord-279463-bli8hwda	150	5	translated	translate	VBN
cord-279463-bli8hwda	150	6	in	in	IN
cord-279463-bli8hwda	150	7	the	the	DT
cord-279463-bli8hwda	150	8	absence	absence	NN
cord-279463-bli8hwda	150	9	or	or	CC
cord-279463-bli8hwda	150	10	presence	presence	NN
cord-279463-bli8hwda	150	11	of	of	IN
cord-279463-bli8hwda	150	12	RM	RM	NNP
cord-279463-bli8hwda	150	13	with	with	IN
cord-279463-bli8hwda	150	14	[	[	-LRB-
cord-279463-bli8hwda	150	15	sH]leutine	sh]leutine	NN
cord-279463-bli8hwda	150	16	as	as	IN
cord-279463-bli8hwda	150	17	label	label	NN
cord-279463-bli8hwda	150	18	.	.	.
cord-279463-bli8hwda	151	1	As	as	IN
cord-279463-bli8hwda	151	2	AN	AN	NNP
cord-279463-bli8hwda	151	3	-	-	:
cord-279463-bli8hwda	151	4	IyCAT	IyCAT	NNP
cord-279463-bli8hwda	151	5	is	be	VBZ
cord-279463-bli8hwda	151	6	essentially	essentially	RB
cord-279463-bli8hwda	151	7	the	the	DT
cord-279463-bli8hwda	151	8	only	only	JJ
cord-279463-bli8hwda	151	9	protein	protein	NN
cord-279463-bli8hwda	151	10	synthesized	synthesize	VBN
cord-279463-bli8hwda	151	11	from	from	IN
cord-279463-bli8hwda	151	12	PAN	pan	NN
cord-279463-bli8hwda	151	13	-	-	HYPH
cord-279463-bli8hwda	151	14	lycat	lycat	NN
cord-279463-bli8hwda	151	15	-	-	HYPH
cord-279463-bli8hwda	151	16	derived	derive	VBN
cord-279463-bli8hwda	151	17	mRNA	mrna	NN
cord-279463-bli8hwda	151	18	,	,	,
cord-279463-bli8hwda	151	19	the	the	DT
cord-279463-bli8hwda	151	20	complete	complete	JJ
cord-279463-bli8hwda	151	21	translation	translation	NN
cord-279463-bli8hwda	151	22	mixture	mixture	NN
cord-279463-bli8hwda	151	23	was	be	VBD
cord-279463-bli8hwda	151	24	subjected	subject	VBN
cord-279463-bli8hwda	151	25	to	to	IN
cord-279463-bli8hwda	151	26	automated	automate	VBN
cord-279463-bli8hwda	151	27	Edman	Edman	NNP
cord-279463-bli8hwda	151	28	degradation	degradation	NN
cord-279463-bli8hwda	151	29	.	.	.
cord-279463-bli8hwda	152	1	As	as	IN
cord-279463-bli8hwda	152	2	seen	see	VBN
cord-279463-bli8hwda	152	3	in	in	IN
cord-279463-bli8hwda	152	4	Figure	figure	NN
cord-279463-bli8hwda	152	5	6A	6a	NN
cord-279463-bli8hwda	152	6	,	,	,
cord-279463-bli8hwda	152	7	leucine	leucine	NN
cord-279463-bli8hwda	152	8	residues	residue	NNS
cord-279463-bli8hwda	152	9	are	be	VBP
cord-279463-bli8hwda	152	10	found	find	VBN
cord-279463-bli8hwda	152	11	at	at	IN
cord-279463-bli8hwda	152	12	the	the	DT
cord-279463-bli8hwda	152	13	positions	position	NNS
cord-279463-bli8hwda	152	14	3	3	CD
cord-279463-bli8hwda	152	15	,	,	,
cord-279463-bli8hwda	152	16	10	10	CD
cord-279463-bli8hwda	152	17	,	,	,
cord-279463-bli8hwda	152	18	13	13	CD
cord-279463-bli8hwda	152	19	,	,	,
cord-279463-bli8hwda	152	20	14	14	CD
cord-279463-bli8hwda	152	21	,	,	,
cord-279463-bli8hwda	152	22	and	and	CC
cord-279463-bli8hwda	152	23	15	15	CD
cord-279463-bli8hwda	152	24	,	,	,
cord-279463-bli8hwda	152	25	as	as	IN
cord-279463-bli8hwda	152	26	predicted	predict	VBN
cord-279463-bli8hwda	152	27	from	from	IN
cord-279463-bli8hwda	152	28	the	the	DT
cord-279463-bli8hwda	152	29	sequence	sequence	NN
cord-279463-bli8hwda	152	30	deduced	deduce	VBN
cord-279463-bli8hwda	152	31	from	from	IN
cord-279463-bli8hwda	152	32	py-2	py-2	NNP
cord-279463-bli8hwda	152	33	cDNA	cdna	NN
cord-279463-bli8hwda	152	34	(	(	-LRB-
cord-279463-bli8hwda	152	35	Claesson	Claesson	NNP
cord-279463-bli8hwda	152	36	et	et	NNP
cord-279463-bli8hwda	152	37	al	al	NNP
cord-279463-bli8hwda	152	38	.	.	NNP
cord-279463-bli8hwda	152	39	,	,	,
cord-279463-bli8hwda	152	40	1983	1983	CD
cord-279463-bli8hwda	152	41	)	)	-RRB-
cord-279463-bli8hwda	152	42	.	.	.
cord-279463-bli8hwda	153	1	The	the	DT
cord-279463-bli8hwda	153	2	initiator	initiator	NN
cord-279463-bli8hwda	153	3	methionine	methionine	NN
cord-279463-bli8hwda	153	4	is	be	VBZ
cord-279463-bli8hwda	153	5	probably	probably	RB
cord-279463-bli8hwda	153	6	removed	remove	VBN
cord-279463-bli8hwda	153	7	during	during	IN
cord-279463-bli8hwda	153	8	or	or	CC
cord-279463-bli8hwda	153	9	shortly	shortly	RB
cord-279463-bli8hwda	153	10	after	after	IN
cord-279463-bli8hwda	153	11	translation	translation	NN
cord-279463-bli8hwda	153	12	(	(	-LRB-
cord-279463-bli8hwda	153	13	Kozak	Kozak	NNP
cord-279463-bli8hwda	153	14	,	,	,
cord-279463-bli8hwda	153	15	1983	1983	CD
cord-279463-bli8hwda	153	16	)	)	-RRB-
cord-279463-bli8hwda	153	17	.	.	.
cord-279463-bli8hwda	154	1	The	the	DT
cord-279463-bli8hwda	154	2	positions	position	NNS
cord-279463-bli8hwda	154	3	of	of	IN
cord-279463-bli8hwda	154	4	leucine	leucine	NN
cord-279463-bli8hwda	154	5	residues	residue	NNS
cord-279463-bli8hwda	154	6	in	in	IN
cord-279463-bli8hwda	154	7	the	the	DT
cord-279463-bli8hwda	154	8	membranetranslocated	membranetranslocate	VBN
cord-279463-bli8hwda	154	9	forms	form	NNS
cord-279463-bli8hwda	154	10	of	of	IN
cord-279463-bli8hwda	154	11	AN	AN	NNP
cord-279463-bli8hwda	154	12	-	-	:
cord-279463-bli8hwda	154	13	I$AT	I$AT	NNPS
cord-279463-bli8hwda	154	14	were	be	VBD
cord-279463-bli8hwda	154	15	similarly	similarly	RB
cord-279463-bli8hwda	154	16	determined	determine	VBN
cord-279463-bli8hwda	154	17	.	.	.
cord-279463-bli8hwda	155	1	As	as	IN
cord-279463-bli8hwda	155	2	RM	RM	NNP
cord-279463-bli8hwda	155	3	in	in	IN
cord-279463-bli8hwda	155	4	the	the	DT
cord-279463-bli8hwda	155	5	in	in	FW
cord-279463-bli8hwda	155	6	vitro	vitro	FW
cord-279463-bli8hwda	155	7	assay	assay	NN
cord-279463-bli8hwda	155	8	do	do	VBP
cord-279463-bli8hwda	155	9	not	not	RB
cord-279463-bli8hwda	155	10	translocate	translocate	VB
cord-279463-bli8hwda	155	11	all	all	DT
cord-279463-bli8hwda	155	12	chains	chain	NNS
cord-279463-bli8hwda	155	13	,	,	,
cord-279463-bli8hwda	155	14	some	some	DT
cord-279463-bli8hwda	155	15	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	155	16	forms	form	NNS
cord-279463-bli8hwda	155	17	remained	remain	VBD
cord-279463-bli8hwda	155	18	(	(	-LRB-
cord-279463-bli8hwda	155	19	see	see	VB
cord-279463-bli8hwda	155	20	inserts	insert	NNS
cord-279463-bli8hwda	155	21	in	in	IN
cord-279463-bli8hwda	155	22	Figures	figure	NNS
cord-279463-bli8hwda	155	23	6A	6a	CD
cord-279463-bli8hwda	155	24	and	and	CC
cord-279463-bli8hwda	155	25	6B	6b	CD
cord-279463-bli8hwda	155	26	)	)	-RRB-
cord-279463-bli8hwda	155	27	.	.	.
cord-279463-bli8hwda	156	1	Leucine	leucine	NN
cord-279463-bli8hwda	156	2	residues	residue	NNS
cord-279463-bli8hwda	156	3	were	be	VBD
cord-279463-bli8hwda	156	4	found	find	VBN
cord-279463-bli8hwda	156	5	at	at	IN
cord-279463-bli8hwda	156	6	positions	position	NNS
cord-279463-bli8hwda	156	7	1,2,3	1,2,3	CD
cord-279463-bli8hwda	156	8	,	,	,
cord-279463-bli8hwda	156	9	and	and	CC
cord-279463-bli8hwda	156	10	13	13	CD
cord-279463-bli8hwda	156	11	(	(	-LRB-
cord-279463-bli8hwda	156	12	Figure	figure	NN
cord-279463-bli8hwda	156	13	66	66	CD
cord-279463-bli8hwda	156	14	)	)	-RRB-
cord-279463-bli8hwda	156	15	.	.	.
cord-279463-bli8hwda	157	1	Larger	large	JJR
cord-279463-bli8hwda	157	2	peaks	peak	NNS
cord-279463-bli8hwda	157	3	at	at	IN
cord-279463-bli8hwda	157	4	positions	position	NNS
cord-279463-bli8hwda	157	5	3	3	CD
cord-279463-bli8hwda	157	6	and	and	CC
cord-279463-bli8hwda	157	7	10	10	CD
cord-279463-bli8hwda	157	8	are	be	VBP
cord-279463-bli8hwda	157	9	consistent	consistent	JJ
cord-279463-bli8hwda	157	10	with	with	IN
cord-279463-bli8hwda	157	11	the	the	DT
cord-279463-bli8hwda	157	12	presence	presence	NN
cord-279463-bli8hwda	157	13	of	of	IN
cord-279463-bli8hwda	157	14	some	some	DT
cord-279463-bli8hwda	157	15	unprocessed	unprocessed	JJ
cord-279463-bli8hwda	157	16	AN	AN	NNP
cord-279463-bli8hwda	157	17	-	-	HYPH
cord-279463-bli8hwda	157	18	IyCAT	IyCAT	NNP
cord-279463-bli8hwda	157	19	(	(	-LRB-
cord-279463-bli8hwda	157	20	see	see	VB
cord-279463-bli8hwda	157	21	insert	insert	NN
cord-279463-bli8hwda	157	22	in	in	IN
cord-279463-bli8hwda	157	23	Figure	figure	NN
cord-279463-bli8hwda	157	24	6B	6b	CD
cord-279463-bli8hwda	157	25	)	)	-RRB-
cord-279463-bli8hwda	157	26	.	.	.
cord-279463-bli8hwda	158	1	Taking	take	VBG
cord-279463-bli8hwda	158	2	into	into	IN
cord-279463-bli8hwda	158	3	account	account	NN
cord-279463-bli8hwda	158	4	the	the	DT
cord-279463-bli8hwda	158	5	size	size	NN
cord-279463-bli8hwda	158	6	reduction	reduction	NN
cord-279463-bli8hwda	158	7	of	of	IN
cord-279463-bli8hwda	158	8	about	about	IN
cord-279463-bli8hwda	158	9	1	1	CD
cord-279463-bli8hwda	158	10	kd	kd	NNS
cord-279463-bli8hwda	158	11	by	by	IN
cord-279463-bli8hwda	158	12	the	the	DT
cord-279463-bli8hwda	158	13	processing	processing	NN
cord-279463-bli8hwda	158	14	13	13	CD
cord-279463-bli8hwda	158	15	,	,	,
cord-279463-bli8hwda	158	16	14	14	CD
cord-279463-bli8hwda	158	17	,	,	,
cord-279463-bli8hwda	158	18	and	and	CC
cord-279463-bli8hwda	158	19	15	15	CD
cord-279463-bli8hwda	158	20	in	in	IN
cord-279463-bli8hwda	158	21	authentic	authentic	JJ
cord-279463-bli8hwda	158	22	AN	AN	NNP
cord-279463-bli8hwda	158	23	-	-	:
cord-279463-bli8hwda	158	24	I$AT	I$AT	NNPS
cord-279463-bli8hwda	158	25	,	,	,
cord-279463-bli8hwda	158	26	we	-PRON-	PRP
cord-279463-bli8hwda	158	27	conclude	conclude	VBP
cord-279463-bli8hwda	158	28	that	that	IN
cord-279463-bli8hwda	158	29	processing	processing	NN
cord-279463-bli8hwda	158	30	has	have	VBZ
cord-279463-bli8hwda	158	31	occurred	occur	VBN
cord-279463-bli8hwda	158	32	between	between	IN
cord-279463-bli8hwda	158	33	amino	amino	NN
cord-279463-bli8hwda	158	34	acid	acid	NN
cord-279463-bli8hwda	158	35	residues	residue	NNS
cord-279463-bli8hwda	158	36	12	12	CD
cord-279463-bli8hwda	158	37	and	and	CC
cord-279463-bli8hwda	158	38	13	13	CD
cord-279463-bli8hwda	159	1	(	(	-LRB-
cord-279463-bli8hwda	159	2	Figures	figure	NNS
cord-279463-bli8hwda	159	3	6B	6b	CD
cord-279463-bli8hwda	159	4	and	and	CC
cord-279463-bli8hwda	159	5	6C	6C	NNP
cord-279463-bli8hwda	159	6	)	)	-RRB-
cord-279463-bli8hwda	159	7	.	.	.
cord-279463-bli8hwda	160	1	Proteolytically	Proteolytically	NNP
cord-279463-bli8hwda	160	2	Processed	process	VBD
cord-279463-bli8hwda	160	3	AN	AN	NNP
cord-279463-bli8hwda	160	4	-	-	HYPH
cord-279463-bli8hwda	160	5	lyCAT	lycat	NN
cord-279463-bli8hwda	161	1	Is	be	VBZ
cord-279463-bli8hwda	161	2	Translocated	Translocated	NNP
cord-279463-bli8hwda	161	3	into	into	IN
cord-279463-bli8hwda	161	4	the	the	DT
cord-279463-bli8hwda	161	5	Lumen	Lumen	NNP
cord-279463-bli8hwda	161	6	of	of	IN
cord-279463-bli8hwda	161	7	Microsomal	Microsomal	NNP
cord-279463-bli8hwda	161	8	Vesicles	Vesicles	NNP
cord-279463-bli8hwda	161	9	With	with	IN
cord-279463-bli8hwda	161	10	the	the	DT
cord-279463-bli8hwda	161	11	proteolytic	proteolytic	JJ
cord-279463-bli8hwda	161	12	removal	removal	NN
cord-279463-bli8hwda	161	13	of	of	IN
cord-279463-bli8hwda	161	14	12	12	CD
cord-279463-bli8hwda	161	15	of	of	IN
cord-279463-bli8hwda	161	16	the	the	DT
cord-279463-bli8hwda	161	17	30	30	CD
cord-279463-bli8hwda	161	18	hydrophobic	hydrophobic	JJ
cord-279463-bli8hwda	161	19	amino	amino	NN
cord-279463-bli8hwda	161	20	acid	acid	NN
cord-279463-bli8hwda	161	21	residues	residue	NNS
cord-279463-bli8hwda	161	22	in	in	IN
cord-279463-bli8hwda	161	23	the	the	DT
cord-279463-bli8hwda	161	24	membrane	membrane	NN
cord-279463-bli8hwda	161	25	-	-	HYPH
cord-279463-bli8hwda	161	26	spanning	span	VBG
cord-279463-bli8hwda	161	27	region	region	NN
cord-279463-bli8hwda	161	28	of	of	IN
cord-279463-bli8hwda	161	29	AN	AN	NNP
cord-279463-bli8hwda	161	30	-	-	HYPH
cord-279463-bli8hwda	161	31	IyCAT	IyCAT	NNP
cord-279463-bli8hwda	162	1	,	,	,
cord-279463-bli8hwda	162	2	the	the	DT
cord-279463-bli8hwda	162	3	question	question	NN
cord-279463-bli8hwda	162	4	arose	arise	VBD
cord-279463-bli8hwda	162	5	as	as	IN
cord-279463-bli8hwda	162	6	to	to	IN
cord-279463-bli8hwda	162	7	whether	whether	IN
cord-279463-bli8hwda	162	8	the	the	DT
cord-279463-bli8hwda	162	9	processed	process	VBN
cord-279463-bli8hwda	162	10	protein	protein	NN
cord-279463-bli8hwda	162	11	was	be	VBD
cord-279463-bli8hwda	162	12	still	still	RB
cord-279463-bli8hwda	162	13	anchored	anchor	VBN
cord-279463-bli8hwda	162	14	in	in	IN
cord-279463-bli8hwda	162	15	the	the	DT
cord-279463-bli8hwda	162	16	membrane	membrane	NN
cord-279463-bli8hwda	162	17	or	or	CC
cord-279463-bli8hwda	162	18	whether	whether	IN
cord-279463-bli8hwda	162	19	it	-PRON-	PRP
cord-279463-bli8hwda	162	20	was	be	VBD
cord-279463-bli8hwda	162	21	now	now	RB
cord-279463-bli8hwda	162	22	released	release	VBN
cord-279463-bli8hwda	162	23	into	into	IN
cord-279463-bli8hwda	162	24	the	the	DT
cord-279463-bli8hwda	162	25	lumen	lumen	NN
cord-279463-bli8hwda	162	26	of	of	IN
cord-279463-bli8hwda	162	27	the	the	DT
cord-279463-bli8hwda	162	28	microsomal	microsomal	NNP
cord-279463-bli8hwda	162	29	vesicles	vesicle	NNS
cord-279463-bli8hwda	162	30	as	as	IN
cord-279463-bli8hwda	162	31	is	be	VBZ
cord-279463-bli8hwda	162	32	the	the	DT
cord-279463-bli8hwda	162	33	case	case	NN
cord-279463-bli8hwda	162	34	for	for	IN
cord-279463-bli8hwda	162	35	secretory	secretory	JJ
cord-279463-bli8hwda	162	36	proteins	protein	NNS
cord-279463-bli8hwda	162	37	.	.	.
cord-279463-bli8hwda	163	1	We	-PRON-	PRP
cord-279463-bli8hwda	163	2	used	use	VBD
cord-279463-bli8hwda	163	3	the	the	DT
cord-279463-bli8hwda	163	4	ex-	ex-	XX
cord-279463-bli8hwda	163	5	tractability	tractability	NN
cord-279463-bli8hwda	163	6	with	with	IN
cord-279463-bli8hwda	163	7	carbonate	carbonate	NN
cord-279463-bli8hwda	163	8	as	as	IN
cord-279463-bli8hwda	163	9	a	a	DT
cord-279463-bli8hwda	163	10	criterion	criterion	NN
cord-279463-bli8hwda	163	11	for	for	IN
cord-279463-bli8hwda	163	12	membrane	membrane	NN
cord-279463-bli8hwda	163	13	integration	integration	NN
cord-279463-bli8hwda	163	14	.	.	.
cord-279463-bli8hwda	164	1	Treatment	treatment	NN
cord-279463-bli8hwda	164	2	of	of	IN
cord-279463-bli8hwda	164	3	RM	RM	NNP
cord-279463-bli8hwda	164	4	with	with	IN
cord-279463-bli8hwda	164	5	carbonate	carbonate	NN
cord-279463-bli8hwda	164	6	at	at	IN
cord-279463-bli8hwda	164	7	pH	pH	NNP
cord-279463-bli8hwda	164	8	11	11	CD
cord-279463-bli8hwda	164	9	releases	release	NNS
cord-279463-bli8hwda	164	10	proteins	protein	NNS
cord-279463-bli8hwda	164	11	that	that	WDT
cord-279463-bli8hwda	164	12	are	be	VBP
cord-279463-bli8hwda	164	13	not	not	RB
cord-279463-bli8hwda	164	14	integrated	integrate	VBN
cord-279463-bli8hwda	164	15	into	into	IN
cord-279463-bli8hwda	164	16	the	the	DT
cord-279463-bli8hwda	164	17	lipid	lipid	NN
cord-279463-bli8hwda	164	18	bilayer	bilayer	NN
cord-279463-bli8hwda	164	19	as	as	RB
cord-279463-bli8hwda	164	20	well	well	RB
cord-279463-bli8hwda	164	21	as	as	IN
cord-279463-bli8hwda	164	22	proteins	protein	NNS
cord-279463-bli8hwda	164	23	present	present	JJ
cord-279463-bli8hwda	164	24	in	in	IN
cord-279463-bli8hwda	164	25	the	the	DT
cord-279463-bli8hwda	164	26	lumen	lumen	NN
cord-279463-bli8hwda	164	27	of	of	IN
cord-279463-bli8hwda	164	28	microsomal	microsomal	NNP
cord-279463-bli8hwda	164	29	vesicles	vesicle	NNS
cord-279463-bli8hwda	164	30	.	.	.
cord-279463-bli8hwda	165	1	AN+CAT	AN+CAT	NNP
cord-279463-bli8hwda	165	2	was	be	VBD
cord-279463-bli8hwda	165	3	translated	translate	VBN
cord-279463-bli8hwda	165	4	in	in	IN
cord-279463-bli8hwda	165	5	the	the	DT
cord-279463-bli8hwda	165	6	presence	presence	NN
cord-279463-bli8hwda	165	7	of	of	IN
cord-279463-bli8hwda	165	8	RM	RM	NNP
cord-279463-bli8hwda	165	9	.	.	.
cord-279463-bli8hwda	166	1	Membranes	membrane	NNS
cord-279463-bli8hwda	166	2	were	be	VBD
cord-279463-bli8hwda	166	3	isolated	isolate	VBN
cord-279463-bli8hwda	166	4	by	by	IN
cord-279463-bli8hwda	166	5	centrifugation	centrifugation	NN
cord-279463-bli8hwda	166	6	through	through	IN
cord-279463-bli8hwda	166	7	a	a	DT
cord-279463-bli8hwda	166	8	sucrose	sucrose	NN
cord-279463-bli8hwda	166	9	cushion	cushion	NN
cord-279463-bli8hwda	166	10	and	and	CC
cord-279463-bli8hwda	166	11	resuspended	resuspend	VBD
cord-279463-bli8hwda	166	12	in	in	IN
cord-279463-bli8hwda	166	13	carbonate	carbonate	NN
cord-279463-bli8hwda	166	14	buffer	buffer	NN
cord-279463-bli8hwda	166	15	.	.	.
cord-279463-bli8hwda	167	1	Solubilized	solubilized	JJ
cord-279463-bli8hwda	167	2	components	component	NNS
cord-279463-bli8hwda	167	3	were	be	VBD
cord-279463-bli8hwda	167	4	then	then	RB
cord-279463-bli8hwda	167	5	separated	separate	VBN
cord-279463-bli8hwda	167	6	from	from	IN
cord-279463-bli8hwda	167	7	membranes	membrane	NNS
cord-279463-bli8hwda	167	8	by	by	IN
cord-279463-bli8hwda	167	9	centrifugation	centrifugation	NN
cord-279463-bli8hwda	167	10	.	.	.
cord-279463-bli8hwda	168	1	Proteins	protein	NNS
cord-279463-bli8hwda	168	2	in	in	IN
cord-279463-bli8hwda	168	3	the	the	DT
cord-279463-bli8hwda	168	4	membrane	membrane	NN
cord-279463-bli8hwda	168	5	pellet	pellet	NN
cord-279463-bli8hwda	168	6	and	and	CC
cord-279463-bli8hwda	168	7	supernatant	supernatant	JJ
cord-279463-bli8hwda	168	8	were	be	VBD
cord-279463-bli8hwda	168	9	analyzed	analyze	VBN
cord-279463-bli8hwda	168	10	by	by	IN
cord-279463-bli8hwda	168	11	SDS	SDS	NNP
cord-279463-bli8hwda	168	12	-	-	HYPH
cord-279463-bli8hwda	168	13	PAGE	PAGE	NNP
cord-279463-bli8hwda	168	14	and	and	CC
cord-279463-bli8hwda	168	15	autoradiography	autoradiography	NN
cord-279463-bli8hwda	168	16	.	.	.
cord-279463-bli8hwda	169	1	Membrane	membrane	NN
cord-279463-bli8hwda	169	2	-	-	HYPH
cord-279463-bli8hwda	169	3	spanning	span	VBG
cord-279463-bli8hwda	169	4	proteins	protein	NNS
cord-279463-bli8hwda	169	5	,	,	,
cord-279463-bli8hwda	169	6	ly	ly	NNP
cord-279463-bli8hwda	169	7	and	and	CC
cord-279463-bli8hwda	169	8	IyCAT	IyCAT	NNP
cord-279463-bli8hwda	169	9	,	,	,
cord-279463-bli8hwda	169	10	and	and	CC
cord-279463-bli8hwda	169	11	the	the	DT
cord-279463-bli8hwda	169	12	secretory	secretory	JJ
cord-279463-bli8hwda	169	13	protein	protein	NN
cord-279463-bli8hwda	169	14	,	,	,
cord-279463-bli8hwda	169	15	mouse	mouse	NN
cord-279463-bli8hwda	169	16	granulocyte	granulocyte	NN
cord-279463-bli8hwda	169	17	-	-	HYPH
cord-279463-bli8hwda	169	18	macrophage	macrophage	NN
cord-279463-bli8hwda	169	19	colony	colony	NN
cord-279463-bli8hwda	169	20	stimulating	stimulate	VBG
cord-279463-bli8hwda	169	21	factor	factor	NN
cord-279463-bli8hwda	169	22	(	(	-LRB-
cord-279463-bli8hwda	169	23	GM	GM	NNP
cord-279463-bli8hwda	169	24	-	-	HYPH
cord-279463-bli8hwda	169	25	CSF	CSF	NNP
cord-279463-bli8hwda	169	26	)	)	-RRB-
cord-279463-bli8hwda	169	27	,	,	,
cord-279463-bli8hwda	169	28	were	be	VBD
cord-279463-bli8hwda	169	29	used	use	VBN
cord-279463-bli8hwda	169	30	as	as	IN
cord-279463-bli8hwda	169	31	control	control	NN
cord-279463-bli8hwda	169	32	(	(	-LRB-
cord-279463-bli8hwda	169	33	Gough	Gough	NNP
cord-279463-bli8hwda	169	34	et	et	NNP
cord-279463-bli8hwda	169	35	al	al	NNP
cord-279463-bli8hwda	169	36	.	.	NNP
cord-279463-bli8hwda	169	37	,	,	,
cord-279463-bli8hwda	169	38	1985	1985	CD
cord-279463-bli8hwda	169	39	)	)	-RRB-
cord-279463-bli8hwda	169	40	.	.	.
cord-279463-bli8hwda	170	1	As	as	IN
cord-279463-bli8hwda	170	2	is	be	VBZ
cord-279463-bli8hwda	170	3	shown	show	VBN
cord-279463-bli8hwda	170	4	in	in	IN
cord-279463-bli8hwda	170	5	Figure	Figure	NNP
cord-279463-bli8hwda	170	6	7	7	CD
cord-279463-bli8hwda	170	7	,	,	,
cord-279463-bli8hwda	170	8	ly	ly	XX
cord-279463-bli8hwda	170	9	"	"	''
cord-279463-bli8hwda	170	10	and	and	CC
cord-279463-bli8hwda	170	11	I-&AT	I-&AT	NNP
cord-279463-bli8hwda	170	12	*	*	NFP
cord-279463-bli8hwda	170	13	,	,	,
cord-279463-bli8hwda	170	14	as	as	IN
cord-279463-bli8hwda	170	15	expected	expect	VBN
cord-279463-bli8hwda	170	16	for	for	IN
cord-279463-bli8hwda	170	17	membrane	membrane	NN
cord-279463-bli8hwda	170	18	-	-	HYPH
cord-279463-bli8hwda	170	19	spanning	span	VBG
cord-279463-bli8hwda	170	20	proteins	protein	NNS
cord-279463-bli8hwda	170	21	,	,	,
cord-279463-bli8hwda	170	22	were	be	VBD
cord-279463-bli8hwda	170	23	found	find	VBN
cord-279463-bli8hwda	170	24	in	in	IN
cord-279463-bli8hwda	170	25	the	the	DT
cord-279463-bli8hwda	170	26	membrane	membrane	NN
cord-279463-bli8hwda	170	27	fraction	fraction	NN
cord-279463-bli8hwda	170	28	.	.	.
cord-279463-bli8hwda	171	1	Both	both	CC
cord-279463-bli8hwda	171	2	AN	AN	NNP
cord-279463-bli8hwda	171	3	-	-	:
cord-279463-bli8hwda	171	4	I$AT	I$AT	NNS
cord-279463-bli8hwda	171	5	'	'	''
cord-279463-bli8hwda	171	6	and	and	CC
cord-279463-bli8hwda	171	7	the	the	DT
cord-279463-bli8hwda	171	8	GM	GM	NNP
cord-279463-bli8hwda	171	9	-	-	HYPH
cord-279463-bli8hwda	171	10	CSF	CSF	NNP
cord-279463-bli8hwda	171	11	'	'	''
cord-279463-bli8hwda	171	12	were	be	VBD
cord-279463-bli8hwda	171	13	found	find	VBN
cord-279463-bli8hwda	171	14	essentially	essentially	RB
cord-279463-bli8hwda	171	15	in	in	IN
cord-279463-bli8hwda	171	16	the	the	DT
cord-279463-bli8hwda	171	17	soluble	soluble	JJ
cord-279463-bli8hwda	171	18	,	,	,
cord-279463-bli8hwda	171	19	carbonatereleased	carbonatereleased	JJ
cord-279463-bli8hwda	171	20	fraction	fraction	NN
cord-279463-bli8hwda	171	21	.	.	.
cord-279463-bli8hwda	172	1	Thus	thus	RB
cord-279463-bli8hwda	172	2	AN	AN	NNP
cord-279463-bli8hwda	172	3	-	-	HYPH
cord-279463-bli8hwda	172	4	IYCAT	IYCAT	NNP
cord-279463-bli8hwda	172	5	'	'	''
cord-279463-bli8hwda	172	6	is	be	VBZ
cord-279463-bli8hwda	172	7	released	release	VBN
cord-279463-bli8hwda	172	8	after	after	IN
cord-279463-bli8hwda	172	9	the	the	DT
cord-279463-bli8hwda	172	10	proteolytic	proteolytic	JJ
cord-279463-bli8hwda	172	11	processing	processing	NN
cord-279463-bli8hwda	172	12	into	into	IN
cord-279463-bli8hwda	172	13	the	the	DT
cord-279463-bli8hwda	172	14	lumen	lumen	NN
cord-279463-bli8hwda	172	15	of	of	IN
cord-279463-bli8hwda	172	16	the	the	DT
cord-279463-bli8hwda	172	17	microsomal	microsomal	NNP
cord-279463-bli8hwda	172	18	vesicles	vesicle	NNS
cord-279463-bli8hwda	172	19	.	.	.
cord-279463-bli8hwda	173	1	Proteolytic	proteolytic	JJ
cord-279463-bli8hwda	173	2	processing	processing	NN
cord-279463-bli8hwda	173	3	,	,	,
cord-279463-bli8hwda	173	4	as	as	IN
cord-279463-bli8hwda	173	5	described	describe	VBN
cord-279463-bli8hwda	173	6	above	above	RB
cord-279463-bli8hwda	173	7	for	for	IN
cord-279463-bli8hwda	173	8	AN	AN	NNP
cord-279463-bli8hwda	173	9	-	-	HYPH
cord-279463-bli8hwda	173	10	IyCAT	IyCAT	NNP
cord-279463-bli8hwda	173	11	,	,	,
cord-279463-bli8hwda	173	12	was	be	VBD
cord-279463-bli8hwda	173	13	also	also	RB
cord-279463-bli8hwda	173	14	obtained	obtain	VBN
cord-279463-bli8hwda	173	15	for	for	IN
cord-279463-bli8hwda	173	16	AN+	AN+	NNP
cord-279463-bli8hwda	173	17	,	,	,
cord-279463-bli8hwda	173	18	a	a	DT
cord-279463-bli8hwda	173	19	protein	protein	NN
cord-279463-bli8hwda	173	20	that	that	WDT
cord-279463-bli8hwda	173	21	lacks	lack	VBZ
cord-279463-bli8hwda	173	22	the	the	DT
cord-279463-bli8hwda	173	23	amino	amino	NN
cord-279463-bli8hwda	173	24	-	-	HYPH
cord-279463-bli8hwda	173	25	terminal	terminal	NN
cord-279463-bli8hwda	173	26	30	30	CD
cord-279463-bli8hwda	173	27	residues	residue	NNS
cord-279463-bli8hwda	173	28	of	of	IN
cord-279463-bli8hwda	173	29	ly	ly	NNP
cord-279463-bli8hwda	173	30	(	(	-LRB-
cord-279463-bli8hwda	173	31	data	datum	NNS
cord-279463-bli8hwda	173	32	not	not	RB
cord-279463-bli8hwda	173	33	shown	show	VBN
cord-279463-bli8hwda	173	34	)	)	-RRB-
cord-279463-bli8hwda	173	35	.	.	.
cord-279463-bli8hwda	174	1	Our	-PRON-	PRP$
cord-279463-bli8hwda	174	2	results	result	NNS
cord-279463-bli8hwda	174	3	show	show	VBP
cord-279463-bli8hwda	174	4	that	that	IN
cord-279463-bli8hwda	174	5	the	the	DT
cord-279463-bli8hwda	174	6	membrane	membrane	NN
cord-279463-bli8hwda	174	7	-	-	HYPH
cord-279463-bli8hwda	174	8	spanning	span	VBG
cord-279463-bli8hwda	174	9	segment	segment	NN
cord-279463-bli8hwda	174	10	of	of	IN
cord-279463-bli8hwda	174	11	the	the	DT
cord-279463-bli8hwda	174	12	type	type	NN
cord-279463-bli8hwda	174	13	II	II	NNP
cord-279463-bli8hwda	174	14	membrane	membrane	NN
cord-279463-bli8hwda	174	15	protein	protein	NN
cord-279463-bli8hwda	174	16	ly	ly	NNP
cord-279463-bli8hwda	174	17	contains	contain	VBZ
cord-279463-bli8hwda	174	18	a	a	DT
cord-279463-bli8hwda	174	19	potentially	potentially	RB
cord-279463-bli8hwda	174	20	cleavable	cleavable	JJ
cord-279463-bli8hwda	174	21	signal	signal	NN
cord-279463-bli8hwda	174	22	sequence	sequence	NN
cord-279463-bli8hwda	174	23	.	.	.
cord-279463-bli8hwda	175	1	This	this	DT
cord-279463-bli8hwda	175	2	signal	signal	NN
cord-279463-bli8hwda	175	3	sequence	sequence	NN
cord-279463-bli8hwda	175	4	is	be	VBZ
cord-279463-bli8hwda	175	5	located	locate	VBN
cord-279463-bli8hwda	175	6	in	in	IN
cord-279463-bli8hwda	175	7	the	the	DT
cord-279463-bli8hwda	175	8	amino	amino	NN
cord-279463-bli8hwda	175	9	-	-	HYPH
cord-279463-bli8hwda	175	10	terminal	terminal	NN
cord-279463-bli8hwda	175	11	half	half	NN
cord-279463-bli8hwda	175	12	of	of	IN
cord-279463-bli8hwda	175	13	the	the	DT
cord-279463-bli8hwda	175	14	membrane	membrane	NN
cord-279463-bli8hwda	175	15	-	-	HYPH
cord-279463-bli8hwda	175	16	spanning	span	VBG
cord-279463-bli8hwda	175	17	segment	segment	NN
cord-279463-bli8hwda	175	18	,	,	,
cord-279463-bli8hwda	175	19	and	and	CC
cord-279463-bli8hwda	175	20	it	-PRON-	PRP
cord-279463-bli8hwda	175	21	is	be	VBZ
cord-279463-bli8hwda	175	22	cleaved	cleave	VBN
cord-279463-bli8hwda	175	23	when	when	WRB
cord-279463-bli8hwda	175	24	the	the	DT
cord-279463-bli8hwda	175	25	preceding	precede	VBG
cord-279463-bli8hwda	175	26	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	175	27	domain	domain	NN
cord-279463-bli8hwda	175	28	is	be	VBZ
cord-279463-bli8hwda	175	29	removed	remove	VBN
cord-279463-bli8hwda	175	30	.	.	.
cord-279463-bli8hwda	176	1	All	all	DT
cord-279463-bli8hwda	176	2	properties	property	NNS
cord-279463-bli8hwda	176	3	known	know	VBN
cord-279463-bli8hwda	176	4	to	to	TO
cord-279463-bli8hwda	176	5	identify	identify	VB
cord-279463-bli8hwda	176	6	a	a	DT
cord-279463-bli8hwda	176	7	signal	signal	JJ
cord-279463-bli8hwda	176	8	sequence	sequence	NN
cord-279463-bli8hwda	176	9	and	and	CC
cord-279463-bli8hwda	176	10	a	a	DT
cord-279463-bli8hwda	176	11	cleavage	cleavage	NN
cord-279463-bli8hwda	176	12	by	by	IN
cord-279463-bli8hwda	176	13	signal	signal	NN
cord-279463-bli8hwda	176	14	peptidase	peptidase	NN
cord-279463-bli8hwda	176	15	can	can	MD
cord-279463-bli8hwda	176	16	be	be	VB
cord-279463-bli8hwda	176	17	demonstrated	demonstrate	VBN
cord-279463-bli8hwda	176	18	.	.	.
cord-279463-bli8hwda	177	1	to	to	TO
cord-279463-bli8hwda	177	2	restrict	restrict	VB
cord-279463-bli8hwda	177	3	vertical	vertical	JJ
cord-279463-bli8hwda	177	4	mobility	mobility	NN
cord-279463-bli8hwda	177	5	of	of	IN
cord-279463-bli8hwda	177	6	the	the	DT
cord-279463-bli8hwda	177	7	membranespanning	membranespanne	VBG
cord-279463-bli8hwda	177	8	segment	segment	NN
cord-279463-bli8hwda	177	9	.	.	.
cord-279463-bli8hwda	178	1	(	(	-LRB-
cord-279463-bli8hwda	178	2	6	6	LS
cord-279463-bli8hwda	178	3	)	)	-RRB-
cord-279463-bli8hwda	178	4	AN	AN	NNP
cord-279463-bli8hwda	178	5	-	-	HYPH
cord-279463-bli8hwda	178	6	ITCAT	ITCAT	NNP
cord-279463-bli8hwda	178	7	,	,	,
cord-279463-bli8hwda	178	8	during	during	IN
cord-279463-bli8hwda	178	9	its	-PRON-	PRP$
cord-279463-bli8hwda	178	10	initial	initial	JJ
cord-279463-bli8hwda	178	11	stage	stage	NN
cord-279463-bli8hwda	178	12	of	of	IN
cord-279463-bli8hwda	178	13	membrane	membrane	NN
cord-279463-bli8hwda	178	14	insertion	insertion	NN
cord-279463-bli8hwda	178	15	,	,	,
cord-279463-bli8hwda	178	16	also	also	RB
cord-279463-bli8hwda	178	17	spans	span	VBZ
cord-279463-bli8hwda	178	18	the	the	DT
cord-279463-bli8hwda	178	19	membrane	membrane	NN
cord-279463-bli8hwda	178	20	with	with	IN
cord-279463-bli8hwda	178	21	its	-PRON-	PRP$
cord-279463-bli8hwda	178	22	hydrophobic	hydrophobic	JJ
cord-279463-bli8hwda	178	23	segment	segment	NN
cord-279463-bli8hwda	178	24	.	.	.
cord-279463-bli8hwda	179	1	However	however	RB
cord-279463-bli8hwda	179	2	,	,	,
cord-279463-bli8hwda	179	3	as	as	IN
cord-279463-bli8hwda	179	4	no	no	DT
cord-279463-bli8hwda	179	5	charged	charge	VBN
cord-279463-bli8hwda	179	6	amino	amino	NN
cord-279463-bli8hwda	179	7	acid	acid	NN
cord-279463-bli8hwda	179	8	residues	residue	NNS
cord-279463-bli8hwda	179	9	are	be	VBP
cord-279463-bli8hwda	179	10	present	present	JJ
cord-279463-bli8hwda	179	11	at	at	IN
cord-279463-bli8hwda	179	12	the	the	DT
cord-279463-bli8hwda	179	13	extreme	extreme	JJ
cord-279463-bli8hwda	179	14	amino	amino	NN
cord-279463-bli8hwda	179	15	-	-	HYPH
cord-279463-bli8hwda	179	16	terminal	terminal	NN
cord-279463-bli8hwda	179	17	end	end	NN
cord-279463-bli8hwda	179	18	,	,	,
cord-279463-bli8hwda	179	19	the	the	DT
cord-279463-bli8hwda	179	20	hydrophobic	hydrophobic	JJ
cord-279463-bli8hwda	179	21	segment	segment	NN
cord-279463-bli8hwda	179	22	has	have	VBZ
cord-279463-bli8hwda	179	23	some	some	DT
cord-279463-bli8hwda	179	24	freedom	freedom	NN
cord-279463-bli8hwda	179	25	to	to	TO
cord-279463-bli8hwda	179	26	change	change	VB
cord-279463-bli8hwda	179	27	its	-PRON-	PRP$
cord-279463-bli8hwda	179	28	topology	topology	NN
cord-279463-bli8hwda	179	29	across	across	IN
cord-279463-bli8hwda	179	30	the	the	DT
cord-279463-bli8hwda	179	31	membrane	membrane	NN
cord-279463-bli8hwda	179	32	.	.	.
cord-279463-bli8hwda	180	1	Part	part	NN
cord-279463-bli8hwda	180	2	of	of	IN
cord-279463-bli8hwda	180	3	the	the	DT
cord-279463-bli8hwda	180	4	hydrophobic	hydrophobic	JJ
cord-279463-bli8hwda	180	5	segment	segment	NN
cord-279463-bli8hwda	180	6	might	may	MD
cord-279463-bli8hwda	180	7	now	now	RB
cord-279463-bli8hwda	180	8	be	be	VB
cord-279463-bli8hwda	180	9	pulled	pull	VBN
cord-279463-bli8hwda	180	10	into	into	IN
cord-279463-bli8hwda	180	11	the	the	DT
cord-279463-bli8hwda	180	12	lumen	lumen	NN
cord-279463-bli8hwda	180	13	of	of	IN
cord-279463-bli8hwda	180	14	the	the	DT
cord-279463-bli8hwda	180	15	ER	ER	NNP
cord-279463-bli8hwda	180	16	membrane	membrane	NN
cord-279463-bli8hwda	180	17	,	,	,
cord-279463-bli8hwda	180	18	and	and	CC
cord-279463-bli8hwda	180	19	a	a	DT
cord-279463-bli8hwda	180	20	former	former	JJ
cord-279463-bli8hwda	180	21	cryptic	cryptic	JJ
cord-279463-bli8hwda	180	22	site	site	NN
cord-279463-bli8hwda	180	23	for	for	IN
cord-279463-bli8hwda	180	24	signal	signal	NN
cord-279463-bli8hwda	180	25	peptidase	peptidase	NN
cord-279463-bli8hwda	180	26	cleavage	cleavage	NN
cord-279463-bli8hwda	180	27	might	may	MD
cord-279463-bli8hwda	180	28	become	become	VB
cord-279463-bli8hwda	180	29	accessible	accessible	JJ
cord-279463-bli8hwda	180	30	to	to	IN
cord-279463-bli8hwda	180	31	the	the	DT
cord-279463-bli8hwda	180	32	active	active	JJ
cord-279463-bli8hwda	180	33	center	center	NN
cord-279463-bli8hwda	180	34	of	of	IN
cord-279463-bli8hwda	180	35	signal	signal	NN
cord-279463-bli8hwda	180	36	peptidase	peptidase	NN
cord-279463-bli8hwda	180	37	.	.	.
cord-279463-bli8hwda	181	1	First	first	RB
cord-279463-bli8hwda	181	2	,	,	,
cord-279463-bli8hwda	181	3	the	the	DT
cord-279463-bli8hwda	181	4	cleavage	cleavage	NN
cord-279463-bli8hwda	181	5	occurs	occur	VBZ
cord-279463-bli8hwda	181	6	concomitant	concomitant	JJ
cord-279463-bli8hwda	181	7	with	with	IN
cord-279463-bli8hwda	181	8	insertion	insertion	NN
cord-279463-bli8hwda	181	9	into	into	IN
cord-279463-bli8hwda	181	10	the	the	DT
cord-279463-bli8hwda	181	11	ER	ER	NNP
cord-279463-bli8hwda	181	12	membrane	membrane	NN
cord-279463-bli8hwda	181	13	as	as	IN
cord-279463-bli8hwda	181	14	is	be	VBZ
cord-279463-bli8hwda	181	15	typical	typical	JJ
cord-279463-bli8hwda	181	16	for	for	IN
cord-279463-bli8hwda	181	17	cleavable	cleavable	JJ
cord-279463-bli8hwda	181	18	signal	signal	NN
cord-279463-bli8hwda	181	19	sequences	sequence	NNS
cord-279463-bli8hwda	181	20	of	of	IN
cord-279463-bli8hwda	181	21	presecretory	presecretory	NN
cord-279463-bli8hwda	181	22	proteins	protein	NNS
cord-279463-bli8hwda	181	23	(	(	-LRB-
cord-279463-bli8hwda	181	24	Blobel	Blobel	NNP
cord-279463-bli8hwda	181	25	and	and	CC
cord-279463-bli8hwda	181	26	Dobberstein	Dobberstein	NNP
cord-279463-bli8hwda	181	27	,	,	,
cord-279463-bli8hwda	181	28	1975	1975	CD
cord-279463-bli8hwda	181	29	)	)	-RRB-
cord-279463-bli8hwda	181	30	.	.	.
cord-279463-bli8hwda	182	1	Second	second	RB
cord-279463-bli8hwda	182	2	,	,	,
cord-279463-bli8hwda	182	3	the	the	DT
cord-279463-bli8hwda	182	4	cleaved	cleave	VBN
cord-279463-bli8hwda	182	5	segment	segment	NN
cord-279463-bli8hwda	182	6	is	be	VBZ
cord-279463-bli8hwda	182	7	located	locate	VBN
cord-279463-bli8hwda	182	8	at	at	IN
cord-279463-bli8hwda	182	9	the	the	DT
cord-279463-bli8hwda	182	10	aminoterminal	aminoterminal	JJ
cord-279463-bli8hwda	182	11	end	end	NN
cord-279463-bli8hwda	182	12	of	of	IN
cord-279463-bli8hwda	182	13	the	the	DT
cord-279463-bli8hwda	182	14	deletion	deletion	NN
cord-279463-bli8hwda	182	15	protein	protein	NN
cord-279463-bli8hwda	182	16	AN	AN	NNP
cord-279463-bli8hwda	182	17	-	-	:
cord-279463-bli8hwda	182	18	IyCAT	IyCAT	NNP
cord-279463-bli8hwda	182	19	.	.	.
cord-279463-bli8hwda	183	1	It	-PRON-	PRP
cord-279463-bli8hwda	183	2	is	be	VBZ
cord-279463-bli8hwda	183	3	13	13	CD
cord-279463-bli8hwda	183	4	amino	amino	NN
cord-279463-bli8hwda	183	5	acid	acid	NN
cord-279463-bli8hwda	183	6	residues	residue	NNS
cord-279463-bli8hwda	183	7	long	long	RB
cord-279463-bli8hwda	183	8	and	and	CC
cord-279463-bli8hwda	183	9	composed	compose	VBD
cord-279463-bli8hwda	183	10	entirely	entirely	RB
cord-279463-bli8hwda	183	11	of	of	IN
cord-279463-bli8hwda	183	12	hydrophobic	hydrophobic	JJ
cord-279463-bli8hwda	183	13	or	or	CC
cord-279463-bli8hwda	183	14	uncharged	uncharged	JJ
cord-279463-bli8hwda	183	15	residues	residue	NNS
cord-279463-bli8hwda	183	16	.	.	.
cord-279463-bli8hwda	184	1	Signal	signal	NN
cord-279463-bli8hwda	184	2	sequences	sequence	NNS
cord-279463-bli8hwda	184	3	can	can	MD
cord-279463-bli8hwda	184	4	vary	vary	VB
cord-279463-bli8hwda	184	5	in	in	IN
cord-279463-bli8hwda	184	6	length	length	NN
cord-279463-bli8hwda	184	7	from	from	IN
cord-279463-bli8hwda	184	8	about	about	RB
cord-279463-bli8hwda	184	9	15	15	CD
cord-279463-bli8hwda	184	10	to	to	IN
cord-279463-bli8hwda	184	11	over	over	IN
cord-279463-bli8hwda	184	12	60	60	CD
cord-279463-bli8hwda	184	13	residues	residue	NNS
cord-279463-bli8hwda	184	14	.	.	.
cord-279463-bli8hwda	185	1	The	the	DT
cord-279463-bli8hwda	185	2	only	only	JJ
cord-279463-bli8hwda	185	3	structural	structural	JJ
cord-279463-bli8hwda	185	4	element	element	NN
cord-279463-bli8hwda	185	5	identified	identify	VBN
cord-279463-bli8hwda	185	6	so	so	RB
cord-279463-bli8hwda	185	7	far	far	RB
cord-279463-bli8hwda	185	8	for	for	IN
cord-279463-bli8hwda	185	9	a	a	DT
cord-279463-bli8hwda	185	10	signal	signal	JJ
cord-279463-bli8hwda	185	11	sequence	sequence	NN
cord-279463-bli8hwda	185	12	is	be	VBZ
cord-279463-bli8hwda	185	13	its	-PRON-	PRP$
cord-279463-bli8hwda	185	14	hydrophobic	hydrophobic	JJ
cord-279463-bli8hwda	185	15	core	core	NN
cord-279463-bli8hwda	185	16	,	,	,
cord-279463-bli8hwda	185	17	usually	usually	RB
cord-279463-bli8hwda	185	18	8	8	CD
cord-279463-bli8hwda	185	19	-	-	SYM
cord-279463-bli8hwda	185	20	12	12	CD
cord-279463-bli8hwda	185	21	residues	residue	NNS
cord-279463-bli8hwda	185	22	long	long	RB
cord-279463-bli8hwda	185	23	.	.	.
cord-279463-bli8hwda	186	1	It	-PRON-	PRP
cord-279463-bli8hwda	186	2	is	be	VBZ
cord-279463-bli8hwda	186	3	followed	follow	VBN
cord-279463-bli8hwda	186	4	by	by	IN
cord-279463-bli8hwda	186	5	a	a	DT
cord-279463-bli8hwda	186	6	more	more	RBR
cord-279463-bli8hwda	186	7	polar	polar	JJ
cord-279463-bli8hwda	186	8	region	region	NN
cord-279463-bli8hwda	186	9	5	5	CD
cord-279463-bli8hwda	186	10	-	-	SYM
cord-279463-bli8hwda	186	11	7	7	CD
cord-279463-bli8hwda	186	12	residues	residue	NNS
cord-279463-bli8hwda	186	13	long	long	RB
cord-279463-bli8hwda	186	14	,	,	,
cord-279463-bli8hwda	186	15	which	which	WDT
cord-279463-bli8hwda	186	16	is	be	VBZ
cord-279463-bli8hwda	186	17	thought	think	VBN
cord-279463-bli8hwda	186	18	to	to	TO
cord-279463-bli8hwda	186	19	define	define	VB
cord-279463-bli8hwda	186	20	the	the	DT
cord-279463-bli8hwda	186	21	cleavage	cleavage	NN
cord-279463-bli8hwda	186	22	site	site	NN
cord-279463-bli8hwda	186	23	for	for	IN
cord-279463-bli8hwda	186	24	signal	signal	NN
cord-279463-bli8hwda	186	25	peptidase	peptidase	NN
cord-279463-bli8hwda	186	26	.	.	.
cord-279463-bli8hwda	187	1	Thus	thus	RB
cord-279463-bli8hwda	187	2	,	,	,
cord-279463-bli8hwda	187	3	a	a	DT
cord-279463-bli8hwda	187	4	"	"	``
cord-279463-bli8hwda	187	5	minimal	minimal	JJ
cord-279463-bli8hwda	187	6	"	"	''
cord-279463-bli8hwda	187	7	signal	signal	JJ
cord-279463-bli8hwda	187	8	sequence	sequence	NN
cord-279463-bli8hwda	187	9	would	would	MD
cord-279463-bli8hwda	187	10	be	be	VB
cord-279463-bli8hwda	187	11	composed	compose	VBN
cord-279463-bli8hwda	187	12	of	of	IN
cord-279463-bli8hwda	187	13	an	an	DT
cord-279463-bli8hwda	187	14	8	8	CD
cord-279463-bli8hwda	187	15	residue	residue	NN
cord-279463-bli8hwda	187	16	hydrophobic	hydrophobic	JJ
cord-279463-bli8hwda	187	17	core	core	NN
cord-279463-bli8hwda	187	18	followed	follow	VBN
cord-279463-bli8hwda	187	19	by	by	IN
cord-279463-bli8hwda	187	20	a	a	DT
cord-279463-bli8hwda	187	21	5	5	CD
cord-279463-bli8hwda	187	22	residue	residue	NN
cord-279463-bli8hwda	187	23	region	region	NN
cord-279463-bli8hwda	187	24	conferring	confer	VBG
cord-279463-bli8hwda	187	25	cleavage	cleavage	NN
cord-279463-bli8hwda	187	26	specificity	specificity	NN
cord-279463-bli8hwda	187	27	(	(	-LRB-
cord-279463-bli8hwda	187	28	von	von	NNP
cord-279463-bli8hwda	187	29	Heijne	Heijne	NNP
cord-279463-bli8hwda	187	30	,	,	,
cord-279463-bli8hwda	187	31	1983	1983	CD
cord-279463-bli8hwda	187	32	(	(	-LRB-
cord-279463-bli8hwda	187	33	von	von	NNP
cord-279463-bli8hwda	187	34	Heijne	Heijne	NNP
cord-279463-bli8hwda	187	35	,	,	,
cord-279463-bli8hwda	187	36	,	,	,
cord-279463-bli8hwda	187	37	1985	1985	CD
cord-279463-bli8hwda	187	38	.	.	.
cord-279463-bli8hwda	188	1	The	the	DT
cord-279463-bli8hwda	188	2	segment	segment	NN
cord-279463-bli8hwda	188	3	cleaved	cleave	VBN
cord-279463-bli8hwda	188	4	from	from	IN
cord-279463-bli8hwda	188	5	protein	protein	NN
cord-279463-bli8hwda	188	6	AN	AN	NNP
cord-279463-bli8hwda	188	7	-	-	:
cord-279463-bli8hwda	188	8	IyCAT	IyCAT	NNP
cord-279463-bli8hwda	188	9	would	would	MD
cord-279463-bli8hwda	188	10	be	be	VB
cord-279463-bli8hwda	188	11	consistent	consistent	JJ
cord-279463-bli8hwda	188	12	with	with	IN
cord-279463-bli8hwda	188	13	such	such	PDT
cord-279463-bli8hwda	188	14	a	a	DT
cord-279463-bli8hwda	188	15	minimal	minimal	JJ
cord-279463-bli8hwda	188	16	length	length	NN
cord-279463-bli8hwda	188	17	signal	signal	NN
cord-279463-bli8hwda	188	18	Sequence	sequence	NN
cord-279463-bli8hwda	188	19	.	.	.
cord-279463-bli8hwda	189	1	Finally	finally	RB
cord-279463-bli8hwda	189	2	,	,	,
cord-279463-bli8hwda	189	3	the	the	DT
cord-279463-bli8hwda	189	4	amino	amino	NN
cord-279463-bli8hwda	189	5	acid	acid	NN
cord-279463-bli8hwda	189	6	residues	residue	NNS
cord-279463-bli8hwda	189	7	around	around	IN
cord-279463-bli8hwda	189	8	the	the	DT
cord-279463-bli8hwda	189	9	cleavage	cleavage	NN
cord-279463-bli8hwda	189	10	site	site	NN
cord-279463-bli8hwda	189	11	in	in	IN
cord-279463-bli8hwda	189	12	membrane	membrane	NN
cord-279463-bli8hwda	189	13	-	-	HYPH
cord-279463-bli8hwda	189	14	translocated	translocate	VBN
cord-279463-bli8hwda	189	15	AN	AN	NNP
cord-279463-bli8hwda	189	16	-	-	HYPH
cord-279463-bli8hwda	189	17	lyCAT	lycat	NN
cord-279463-bli8hwda	190	1	*	*	NFP
cord-279463-bli8hwda	190	2	'	'	''
cord-279463-bli8hwda	190	3	are	be	VBP
cord-279463-bli8hwda	190	4	consistent	consistent	JJ
cord-279463-bli8hwda	190	5	with	with	IN
cord-279463-bli8hwda	190	6	cleavage	cleavage	NN
cord-279463-bli8hwda	190	7	by	by	IN
cord-279463-bli8hwda	190	8	signal	signal	NN
cord-279463-bli8hwda	190	9	peptidase	peptidase	NN
cord-279463-bli8hwda	190	10	.	.	.
cord-279463-bli8hwda	191	1	Based	base	VBN
cord-279463-bli8hwda	191	2	on	on	IN
cord-279463-bli8hwda	191	3	a	a	DT
cord-279463-bli8hwda	191	4	sequence	sequence	NN
cord-279463-bli8hwda	191	5	comparison	comparison	NN
cord-279463-bli8hwda	191	6	of	of	IN
cord-279463-bli8hwda	191	7	78	78	CD
cord-279463-bli8hwda	191	8	eukaryotic	eukaryotic	JJ
cord-279463-bli8hwda	191	9	signal	signal	NN
cord-279463-bli8hwda	191	10	sequences	sequence	NNS
cord-279463-bli8hwda	191	11	,	,	,
cord-279463-bli8hwda	191	12	von	von	NNP
cord-279463-bli8hwda	191	13	Heijne	Heijne	NNP
cord-279463-bli8hwda	191	14	found	find	VBD
cord-279463-bli8hwda	191	15	that	that	IN
cord-279463-bli8hwda	191	16	only	only	JJ
cord-279463-bli8hwda	191	17	small	small	JJ
cord-279463-bli8hwda	191	18	neutral	neutral	JJ
cord-279463-bli8hwda	191	19	residues	residue	NNS
cord-279463-bli8hwda	191	20	are	be	VBP
cord-279463-bli8hwda	191	21	found	find	VBN
cord-279463-bli8hwda	191	22	at	at	IN
cord-279463-bli8hwda	191	23	the	the	DT
cord-279463-bli8hwda	191	24	site	site	NN
cord-279463-bli8hwda	191	25	of	of	IN
cord-279463-bli8hwda	191	26	cleavage	cleavage	NN
cord-279463-bli8hwda	191	27	(	(	-LRB-
cord-279463-bli8hwda	191	28	-1	-1	NN
cord-279463-bli8hwda	191	29	position	position	NN
cord-279463-bli8hwda	191	30	)	)	-RRB-
cord-279463-bli8hwda	191	31	and	and	CC
cord-279463-bli8hwda	191	32	that	that	IN
cord-279463-bli8hwda	191	33	only	only	JJ
cord-279463-bli8hwda	191	34	small	small	JJ
cord-279463-bli8hwda	191	35	neutral	neutral	JJ
cord-279463-bli8hwda	191	36	and	and	CC
cord-279463-bli8hwda	191	37	uncharged	uncharged	JJ
cord-279463-bli8hwda	191	38	ones	one	NNS
cord-279463-bli8hwda	191	39	are	be	VBP
cord-279463-bli8hwda	191	40	found	find	VBN
cord-279463-bli8hwda	191	41	at	at	IN
cord-279463-bli8hwda	191	42	the	the	DT
cord-279463-bli8hwda	191	43	-3	-3	NNP
cord-279463-bli8hwda	191	44	position	position	NN
cord-279463-bli8hwda	191	45	,	,	,
cord-279463-bli8hwda	191	46	that	that	RB
cord-279463-bli8hwda	191	47	is	be	VBZ
cord-279463-bli8hwda	191	48	3	3	CD
cord-279463-bli8hwda	191	49	amino	amino	NN
cord-279463-bli8hwda	191	50	acid	acid	NN
cord-279463-bli8hwda	191	51	residues	residue	NNS
cord-279463-bli8hwda	191	52	in	in	IN
cord-279463-bli8hwda	191	53	front	front	NN
cord-279463-bli8hwda	191	54	of	of	IN
cord-279463-bli8hwda	191	55	the	the	DT
cord-279463-bli8hwda	191	56	signal	signal	JJ
cord-279463-bli8hwda	191	57	peptidase	peptidase	NN
cord-279463-bli8hwda	191	58	cleavage	cleavage	NN
cord-279463-bli8hwda	191	59	site	site	NN
cord-279463-bli8hwda	191	60	(	(	-LRB-
cord-279463-bli8hwda	191	61	von	von	NNP
cord-279463-bli8hwda	191	62	Heijne	Heijne	NNP
cord-279463-bli8hwda	191	63	,	,	,
cord-279463-bli8hwda	191	64	1983	1983	CD
cord-279463-bli8hwda	191	65	)	)	-RRB-
cord-279463-bli8hwda	191	66	.	.	.
cord-279463-bli8hwda	192	1	In	in	IN
cord-279463-bli8hwda	192	2	the	the	DT
cord-279463-bli8hwda	192	3	segment	segment	NN
cord-279463-bli8hwda	192	4	cleaved	cleave	VBN
cord-279463-bli8hwda	192	5	from	from	IN
cord-279463-bli8hwda	192	6	AN	AN	NNP
cord-279463-bli8hwda	192	7	-	-	HYPH
cord-279463-bli8hwda	192	8	I$AT	I$AT	NNS
cord-279463-bli8hwda	192	9	,	,	,
cord-279463-bli8hwda	192	10	threonine	threonine	NN
cord-279463-bli8hwda	192	11	,	,	,
cord-279463-bli8hwda	192	12	a	a	DT
cord-279463-bli8hwda	192	13	small	small	JJ
cord-279463-bli8hwda	192	14	neutral	neutral	JJ
cord-279463-bli8hwda	192	15	amino	amino	NN
cord-279463-bli8hwda	192	16	acid	acid	NN
cord-279463-bli8hwda	192	17	,	,	,
cord-279463-bli8hwda	192	18	is	be	VBZ
cord-279463-bli8hwda	192	19	found	find	VBN
cord-279463-bli8hwda	192	20	at	at	IN
cord-279463-bli8hwda	192	21	the	the	DT
cord-279463-bli8hwda	192	22	-1	-1	NN
cord-279463-bli8hwda	192	23	position	position	NN
cord-279463-bli8hwda	192	24	,	,	,
cord-279463-bli8hwda	192	25	and	and	CC
cord-279463-bli8hwda	192	26	leucine	leucine	NN
cord-279463-bli8hwda	192	27	,	,	,
cord-279463-bli8hwda	192	28	an	an	DT
cord-279463-bli8hwda	192	29	uncharged	uncharged	JJ
cord-279463-bli8hwda	192	30	amino	amino	NN
cord-279463-bli8hwda	192	31	acid	acid	NN
cord-279463-bli8hwda	192	32	,	,	,
cord-279463-bli8hwda	192	33	at	at	IN
cord-279463-bli8hwda	192	34	the	the	DT
cord-279463-bli8hwda	192	35	-3	-3	NNP
cord-279463-bli8hwda	192	36	position	position	NN
cord-279463-bli8hwda	192	37	.	.	.
cord-279463-bli8hwda	193	1	Both	both	DT
cord-279463-bli8hwda	193	2	of	of	IN
cord-279463-bli8hwda	193	3	these	these	DT
cord-279463-bli8hwda	193	4	residues	residue	NNS
cord-279463-bli8hwda	193	5	fulfill	fulfill	VBP
cord-279463-bli8hwda	193	6	the	the	DT
cord-279463-bli8hwda	193	7	above	above	RB
cord-279463-bli8hwda	193	8	described	describe	VBN
cord-279463-bli8hwda	193	9	criteria	criterion	NNS
cord-279463-bli8hwda	193	10	for	for	IN
cord-279463-bli8hwda	193	11	a	a	DT
cord-279463-bli8hwda	193	12	signal	signal	JJ
cord-279463-bli8hwda	193	13	peptidase	peptidase	NN
cord-279463-bli8hwda	193	14	cleavage	cleavage	NN
cord-279463-bli8hwda	193	15	site	site	NN
cord-279463-bli8hwda	193	16	.	.	.
cord-279463-bli8hwda	194	1	Thus	thus	RB
cord-279463-bli8hwda	194	2	,	,	,
cord-279463-bli8hwda	194	3	place	place	NN
cord-279463-bli8hwda	194	4	(	(	-LRB-
cord-279463-bli8hwda	194	5	RM	RM	NNP
cord-279463-bli8hwda	194	6	)	)	-RRB-
cord-279463-bli8hwda	194	7	and	and	CC
cord-279463-bli8hwda	194	8	time	time	NN
cord-279463-bli8hwda	194	9	of	of	IN
cord-279463-bli8hwda	194	10	cleavage	cleavage	NN
cord-279463-bli8hwda	194	11	(	(	-LRB-
cord-279463-bli8hwda	194	12	cotranslational	cotranslational	NNP
cord-279463-bli8hwda	194	13	)	)	-RRB-
cord-279463-bli8hwda	194	14	,	,	,
cord-279463-bli8hwda	194	15	hydrophobic	hydrophobic	JJ
cord-279463-bli8hwda	194	16	character	character	NN
cord-279463-bli8hwda	194	17	of	of	IN
cord-279463-bli8hwda	194	18	the	the	DT
cord-279463-bli8hwda	194	19	cleaved	cleave	VBN
cord-279463-bli8hwda	194	20	segment	segment	NN
cord-279463-bli8hwda	194	21	,	,	,
cord-279463-bli8hwda	194	22	and	and	CC
cord-279463-bli8hwda	194	23	property	property	NN
cord-279463-bli8hwda	194	24	of	of	IN
cord-279463-bli8hwda	194	25	the	the	DT
cord-279463-bli8hwda	194	26	cleavage	cleavage	NN
cord-279463-bli8hwda	194	27	site	site	NN
cord-279463-bli8hwda	194	28	demonstrate	demonstrate	VBP
cord-279463-bli8hwda	194	29	that	that	IN
cord-279463-bli8hwda	194	30	AN	AN	NNP
cord-279463-bli8hwda	194	31	-	-	:
cord-279463-bli8hwda	194	32	I$AT	I$AT	NNP
cord-279463-bli8hwda	194	33	contains	contain	VBZ
cord-279463-bli8hwda	194	34	a	a	DT
cord-279463-bli8hwda	194	35	signal	signal	JJ
cord-279463-bli8hwda	194	36	sequence	sequence	NN
cord-279463-bli8hwda	194	37	at	at	IN
cord-279463-bli8hwda	194	38	its	-PRON-	PRP$
cord-279463-bli8hwda	194	39	amino	amino	NN
cord-279463-bli8hwda	194	40	terminus	terminus	NN
cord-279463-bli8hwda	194	41	which	which	WDT
cord-279463-bli8hwda	194	42	is	be	VBZ
cord-279463-bli8hwda	194	43	cleaved	cleave	VBN
cord-279463-bli8hwda	194	44	upon	upon	IN
cord-279463-bli8hwda	194	45	membrane	membrane	NN
cord-279463-bli8hwda	194	46	insertion	insertion	NN
cord-279463-bli8hwda	194	47	by	by	IN
cord-279463-bli8hwda	194	48	signal	signal	NN
cord-279463-bli8hwda	194	49	peptidase	peptidase	NN
cord-279463-bli8hwda	194	50	.	.	.
cord-279463-bli8hwda	195	1	How	how	WRB
cord-279463-bli8hwda	195	2	can	can	MD
cord-279463-bli8hwda	195	3	we	-PRON-	PRP
cord-279463-bli8hwda	195	4	possibly	possibly	RB
cord-279463-bli8hwda	195	5	explain	explain	VB
cord-279463-bli8hwda	195	6	how	how	WRB
cord-279463-bli8hwda	195	7	the	the	DT
cord-279463-bli8hwda	195	8	deletion	deletion	NN
cord-279463-bli8hwda	195	9	of	of	IN
cord-279463-bli8hwda	195	10	the	the	DT
cord-279463-bli8hwda	195	11	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	195	12	,	,	,
cord-279463-bli8hwda	195	13	hydrophilic	hydrophilic	JJ
cord-279463-bli8hwda	195	14	segment	segment	NN
cord-279463-bli8hwda	195	15	from	from	IN
cord-279463-bli8hwda	195	16	IyCAT	IyCAT	NNP
cord-279463-bli8hwda	195	17	reveals	reveal	VBZ
cord-279463-bli8hwda	195	18	a	a	DT
cord-279463-bli8hwda	195	19	cleavable	cleavable	JJ
cord-279463-bli8hwda	195	20	signal	signal	NN
cord-279463-bli8hwda	195	21	sequence	sequence	NN
cord-279463-bli8hwda	195	22	in	in	IN
cord-279463-bli8hwda	195	23	a	a	DT
cord-279463-bli8hwda	195	24	formerly	formerly	RB
cord-279463-bli8hwda	195	25	membranespanning	membranespanne	VBG
cord-279463-bli8hwda	195	26	region	region	NN
cord-279463-bli8hwda	195	27	?	?	.
cord-279463-bli8hwda	196	1	To	to	IN
cord-279463-bli8hwda	196	2	us	-PRON-	PRP
cord-279463-bli8hwda	196	3	the	the	DT
cord-279463-bli8hwda	196	4	most	most	RBS
cord-279463-bli8hwda	196	5	plausible	plausible	JJ
cord-279463-bli8hwda	196	6	explanation	explanation	NN
cord-279463-bli8hwda	196	7	is	be	VBZ
cord-279463-bli8hwda	196	8	that	that	IN
cord-279463-bli8hwda	196	9	the	the	DT
cord-279463-bli8hwda	196	10	position	position	NN
cord-279463-bli8hwda	196	11	of	of	IN
cord-279463-bli8hwda	196	12	the	the	DT
cord-279463-bli8hwda	196	13	hydrophobic	hydrophobic	JJ
cord-279463-bli8hwda	196	14	segment	segment	NN
cord-279463-bli8hwda	196	15	in	in	IN
cord-279463-bli8hwda	196	16	the	the	DT
cord-279463-bli8hwda	196	17	membrane	membrane	NN
cord-279463-bli8hwda	196	18	is	be	VBZ
cord-279463-bli8hwda	196	19	different	different	JJ
cord-279463-bli8hwda	196	20	in	in	IN
cord-279463-bli8hwda	196	21	IyCAT	IyCAT	NNP
cord-279463-bli8hwda	196	22	and	and	CC
cord-279463-bli8hwda	196	23	AN	AN	NNP
cord-279463-bli8hwda	196	24	-	-	:
cord-279463-bli8hwda	196	25	I$AT	I$AT	NNP
cord-279463-bli8hwda	196	26	Signal	Signal	NNP
cord-279463-bli8hwda	196	27	peptidase	peptidase	NN
cord-279463-bli8hwda	196	28	is	be	VBZ
cord-279463-bli8hwda	196	29	known	know	VBN
cord-279463-bli8hwda	196	30	to	to	TO
cord-279463-bli8hwda	196	31	be	be	VB
cord-279463-bli8hwda	196	32	an	an	DT
cord-279463-bli8hwda	196	33	integral	integral	JJ
cord-279463-bli8hwda	196	34	membrane	membrane	NN
cord-279463-bli8hwda	196	35	protein	protein	NN
cord-279463-bli8hwda	196	36	not	not	RB
cord-279463-bli8hwda	196	37	exposed	expose	VBN
cord-279463-bli8hwda	196	38	on	on	IN
cord-279463-bli8hwda	196	39	the	the	DT
cord-279463-bli8hwda	196	40	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	196	41	side	side	NN
cord-279463-bli8hwda	196	42	of	of	IN
cord-279463-bli8hwda	196	43	RM	RM	NNP
cord-279463-bli8hwda	196	44	(	(	-LRB-
cord-279463-bli8hwda	196	45	Jackson	Jackson	NNP
cord-279463-bli8hwda	196	46	and	and	CC
cord-279463-bli8hwda	196	47	Blobel	Blobel	NNP
cord-279463-bli8hwda	196	48	,	,	,
cord-279463-bli8hwda	196	49	1977	1977	CD
cord-279463-bli8hwda	196	50	;	;	:
cord-279463-bli8hwda	196	51	Lively	lively	JJ
cord-279463-bli8hwda	196	52	and	and	CC
cord-279463-bli8hwda	196	53	Walsh	Walsh	NNP
cord-279463-bli8hwda	196	54	,	,	,
cord-279463-bli8hwda	196	55	1983	1983	CD
cord-279463-bli8hwda	196	56	;	;	:
cord-279463-bli8hwda	196	57	Evans	Evans	NNP
cord-279463-bli8hwda	196	58	et	et	NNP
cord-279463-bli8hwda	196	59	al	al	NNP
cord-279463-bli8hwda	196	60	.	.	NNP
cord-279463-bli8hwda	196	61	,	,	,
cord-279463-bli8hwda	196	62	1986	1986	CD
cord-279463-bli8hwda	196	63	)	)	-RRB-
cord-279463-bli8hwda	196	64	.	.	.
cord-279463-bli8hwda	197	1	As	as	IN
cord-279463-bli8hwda	197	2	in	in	IN
cord-279463-bli8hwda	197	3	many	many	JJ
cord-279463-bli8hwda	197	4	secretory	secretory	JJ
cord-279463-bli8hwda	197	5	proteins	protein	NNS
cord-279463-bli8hwda	197	6	,	,	,
cord-279463-bli8hwda	197	7	the	the	DT
cord-279463-bli8hwda	197	8	cleavage	cleavage	NN
cord-279463-bli8hwda	197	9	site	site	NN
cord-279463-bli8hwda	197	10	for	for	IN
cord-279463-bli8hwda	197	11	signal	signal	NN
cord-279463-bli8hwda	197	12	peptidase	peptidase	NN
cord-279463-bli8hwda	197	13	is	be	VBZ
cord-279463-bli8hwda	197	14	surrounded	surround	VBN
cord-279463-bli8hwda	197	15	on	on	IN
cord-279463-bli8hwda	197	16	either	either	DT
cord-279463-bli8hwda	197	17	side	side	NN
cord-279463-bli8hwda	197	18	by	by	IN
cord-279463-bli8hwda	197	19	1	1	CD
cord-279463-bli8hwda	197	20	or	or	CC
cord-279463-bli8hwda	197	21	even	even	RB
cord-279463-bli8hwda	197	22	2	2	CD
cord-279463-bli8hwda	197	23	charged	charge	VBN
cord-279463-bli8hwda	197	24	amino	amino	NN
cord-279463-bli8hwda	197	25	acid	acid	NN
cord-279463-bli8hwda	197	26	residues	residue	NNS
cord-279463-bli8hwda	197	27	.	.	.
cord-279463-bli8hwda	198	1	It	-PRON-	PRP
cord-279463-bli8hwda	198	2	is	be	VBZ
cord-279463-bli8hwda	198	3	reasonable	reasonable	JJ
cord-279463-bli8hwda	198	4	to	to	TO
cord-279463-bli8hwda	198	5	assume	assume	VB
cord-279463-bli8hwda	198	6	that	that	IN
cord-279463-bli8hwda	198	7	the	the	DT
cord-279463-bli8hwda	198	8	active	active	JJ
cord-279463-bli8hwda	198	9	center	center	NN
cord-279463-bli8hwda	198	10	of	of	IN
cord-279463-bli8hwda	198	11	this	this	DT
cord-279463-bli8hwda	198	12	enzyme	enzyme	NN
cord-279463-bli8hwda	198	13	is	be	VBZ
cord-279463-bli8hwda	198	14	located	locate	VBN
cord-279463-bli8hwda	198	15	close	close	RB
cord-279463-bli8hwda	198	16	to	to	IN
cord-279463-bli8hwda	198	17	the	the	DT
cord-279463-bli8hwda	198	18	exoplasmic	exoplasmic	JJ
cord-279463-bli8hwda	198	19	side	side	NN
cord-279463-bli8hwda	198	20	of	of	IN
cord-279463-bli8hwda	198	21	the	the	DT
cord-279463-bli8hwda	198	22	ER	ER	NNP
cord-279463-bli8hwda	198	23	membrane	membrane	NN
cord-279463-bli8hwda	198	24	,	,	,
cord-279463-bli8hwda	198	25	not	not	RB
cord-279463-bli8hwda	198	26	within	within	IN
cord-279463-bli8hwda	198	27	the	the	DT
cord-279463-bli8hwda	198	28	membrane	membrane	NN
cord-279463-bli8hwda	198	29	.	.	.
cord-279463-bli8hwda	199	1	We	-PRON-	PRP
cord-279463-bli8hwda	199	2	propose	propose	VBP
cord-279463-bli8hwda	199	3	that	that	IN
cord-279463-bli8hwda	199	4	the	the	DT
cord-279463-bli8hwda	199	5	removal	removal	NN
cord-279463-bli8hwda	199	6	of	of	IN
cord-279463-bli8hwda	199	7	the	the	DT
cord-279463-bli8hwda	199	8	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	199	9	,	,	,
cord-279463-bli8hwda	199	10	hydrophilic	hydrophilic	JJ
cord-279463-bli8hwda	199	11	segment	segment	NN
cord-279463-bli8hwda	199	12	from	from	IN
cord-279463-bli8hwda	199	13	IyCAT	IyCAT	NNP
cord-279463-bli8hwda	199	14	allows	allow	VBZ
cord-279463-bli8hwda	199	15	the	the	DT
cord-279463-bli8hwda	199	16	hydrophobic	hydrophobic	JJ
cord-279463-bli8hwda	199	17	segment	segment	NN
cord-279463-bli8hwda	199	18	to	to	TO
cord-279463-bli8hwda	199	19	shift	shift	VB
cord-279463-bli8hwda	199	20	its	-PRON-	PRP$
cord-279463-bli8hwda	199	21	position	position	NN
cord-279463-bli8hwda	199	22	within	within	IN
cord-279463-bli8hwda	199	23	the	the	DT
cord-279463-bli8hwda	199	24	ER	ER	NNP
cord-279463-bli8hwda	199	25	membrane	membrane	NN
cord-279463-bli8hwda	199	26	.	.	.
cord-279463-bli8hwda	200	1	Most	most	RBS
cord-279463-bli8hwda	200	2	likely	likely	RB
cord-279463-bli8hwda	200	3	it	-PRON-	PRP
cord-279463-bli8hwda	200	4	positions	position	VBZ
cord-279463-bli8hwda	200	5	itself	-PRON-	PRP
cord-279463-bli8hwda	200	6	more	more	RBR
cord-279463-bli8hwda	200	7	toward	toward	IN
cord-279463-bli8hwda	200	8	the	the	DT
cord-279463-bli8hwda	200	9	exoplasmic	exoplasmic	JJ
cord-279463-bli8hwda	200	10	side	side	NN
cord-279463-bli8hwda	200	11	.	.	.
cord-279463-bli8hwda	201	1	Hence	hence	RB
cord-279463-bli8hwda	201	2	,	,	,
cord-279463-bli8hwda	201	3	a	a	DT
cord-279463-bli8hwda	201	4	potential	potential	JJ
cord-279463-bli8hwda	201	5	signal	signal	NN
cord-279463-bli8hwda	201	6	peptidase	peptidase	NN
cord-279463-bli8hwda	201	7	cleavage	cleavage	NN
cord-279463-bli8hwda	201	8	site	site	NN
cord-279463-bli8hwda	201	9	becomes	become	VBZ
cord-279463-bli8hwda	201	10	accessible	accessible	JJ
cord-279463-bli8hwda	201	11	to	to	IN
cord-279463-bli8hwda	201	12	the	the	DT
cord-279463-bli8hwda	201	13	active	active	JJ
cord-279463-bli8hwda	201	14	center	center	NN
cord-279463-bli8hwda	201	15	of	of	IN
cord-279463-bli8hwda	201	16	signal	signal	NN
cord-279463-bli8hwda	201	17	peptidase	peptidase	NN
cord-279463-bli8hwda	201	18	(	(	-LRB-
cord-279463-bli8hwda	201	19	see	see	VB
cord-279463-bli8hwda	201	20	Figure	figure	NN
cord-279463-bli8hwda	201	21	8B	8b	NN
cord-279463-bli8hwda	201	22	)	)	-RRB-
cord-279463-bli8hwda	201	23	.	.	.
cord-279463-bli8hwda	202	1	It	-PRON-	PRP
cord-279463-bli8hwda	202	2	has	have	VBZ
cord-279463-bli8hwda	202	3	been	be	VBN
cord-279463-bli8hwda	202	4	noted	note	VBN
cord-279463-bli8hwda	202	5	previously	previously	RB
cord-279463-bli8hwda	202	6	in	in	IN
cord-279463-bli8hwda	202	7	type	type	NN
cord-279463-bli8hwda	202	8	I	-PRON-	PRP
cord-279463-bli8hwda	202	9	membrane	membrane	NN
cord-279463-bli8hwda	202	10	proteins	protein	NNS
cord-279463-bli8hwda	202	11	that	that	IN
cord-279463-bli8hwda	202	12	a	a	DT
cord-279463-bli8hwda	202	13	deletion	deletion	NN
cord-279463-bli8hwda	202	14	of	of	IN
cord-279463-bli8hwda	202	15	the	the	DT
cord-279463-bli8hwda	202	16	charged	charge	VBN
cord-279463-bli8hwda	202	17	amino	amino	NN
cord-279463-bli8hwda	202	18	acid	acid	NN
cord-279463-bli8hwda	202	19	residues	residue	NNS
cord-279463-bli8hwda	202	20	flanking	flank	VBG
cord-279463-bli8hwda	202	21	the	the	DT
cord-279463-bli8hwda	202	22	membrane	membrane	NN
cord-279463-bli8hwda	202	23	-	-	HYPH
cord-279463-bli8hwda	202	24	spanning	span	VBG
cord-279463-bli8hwda	202	25	region	region	NN
cord-279463-bli8hwda	202	26	does	do	VBZ
cord-279463-bli8hwda	202	27	not	not	RB
cord-279463-bli8hwda	202	28	affect	affect	VB
cord-279463-bli8hwda	202	29	the	the	DT
cord-279463-bli8hwda	202	30	overall	overall	JJ
cord-279463-bli8hwda	202	31	topology	topology	NN
cord-279463-bli8hwda	202	32	(	(	-LRB-
cord-279463-bli8hwda	202	33	Zuninga	Zuninga	NNP
cord-279463-bli8hwda	202	34	and	and	CC
cord-279463-bli8hwda	202	35	Hood	Hood	NNP
cord-279463-bli8hwda	202	36	,	,	,
cord-279463-bli8hwda	202	37	1986	1986	CD
cord-279463-bli8hwda	202	38	;	;	.
cord-279463-bli8hwda	203	1	Cutler	Cutler	NNP
cord-279463-bli8hwda	203	2	et	et	NNP
cord-279463-bli8hwda	203	3	al	al	NNP
cord-279463-bli8hwda	203	4	.	.	NNP
cord-279463-bli8hwda	203	5	,	,	,
cord-279463-bli8hwda	203	6	1986	1986	CD
cord-279463-bli8hwda	203	7	)	)	-RRB-
cord-279463-bli8hwda	203	8	.	.	.
cord-279463-bli8hwda	204	1	In	in	IN
cord-279463-bli8hwda	204	2	the	the	DT
cord-279463-bli8hwda	204	3	case	case	NN
cord-279463-bli8hwda	204	4	of	of	IN
cord-279463-bli8hwda	204	5	Es	Es	NNP
cord-279463-bli8hwda	204	6	glycoprotein	glycoprotein	NN
cord-279463-bli8hwda	204	7	of	of	IN
cord-279463-bli8hwda	204	8	Semliki	Semliki	NNP
cord-279463-bli8hwda	204	9	Forest	Forest	NNP
cord-279463-bli8hwda	204	10	virus	virus	NN
cord-279463-bli8hwda	204	11	,	,	,
cord-279463-bli8hwda	204	12	it	-PRON-	PRP
cord-279463-bli8hwda	204	13	has	have	VBZ
cord-279463-bli8hwda	204	14	been	be	VBN
cord-279463-bli8hwda	204	15	shown	show	VBN
cord-279463-bli8hwda	204	16	that	that	IN
cord-279463-bli8hwda	204	17	mutation	mutation	NN
cord-279463-bli8hwda	204	18	of	of	IN
cord-279463-bli8hwda	204	19	the	the	DT
cord-279463-bli8hwda	204	20	basic	basic	JJ
cord-279463-bli8hwda	204	21	amino	amino	NN
cord-279463-bli8hwda	204	22	acid	acid	NN
cord-279463-bli8hwda	204	23	residues	residue	NNS
cord-279463-bli8hwda	204	24	at	at	IN
cord-279463-bli8hwda	204	25	the	the	DT
cord-279463-bli8hwda	204	26	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	204	27	side	side	NN
cord-279463-bli8hwda	204	28	of	of	IN
cord-279463-bli8hwda	204	29	the	the	DT
cord-279463-bli8hwda	204	30	membranespanning	membranespanne	VBG
cord-279463-bli8hwda	204	31	segment	segment	NN
cord-279463-bli8hwda	204	32	reduces	reduce	VBZ
cord-279463-bli8hwda	204	33	the	the	DT
cord-279463-bli8hwda	204	34	stability	stability	NN
cord-279463-bli8hwda	204	35	of	of	IN
cord-279463-bli8hwda	204	36	the	the	DT
cord-279463-bli8hwda	204	37	mutant	mutant	JJ
cord-279463-bli8hwda	204	38	protein	protein	NN
cord-279463-bli8hwda	204	39	in	in	IN
cord-279463-bli8hwda	204	40	the	the	DT
cord-279463-bli8hwda	204	41	membrane	membrane	NN
cord-279463-bli8hwda	205	1	(	(	-LRB-
cord-279463-bli8hwda	205	2	Cutler	Cutler	NNP
cord-279463-bli8hwda	205	3	et	et	NNP
cord-279463-bli8hwda	205	4	al	al	NNP
cord-279463-bli8hwda	205	5	.	.	NNP
cord-279463-bli8hwda	205	6	,	,	,
cord-279463-bli8hwda	205	7	1986	1986	CD
cord-279463-bli8hwda	205	8	)	)	-RRB-
cord-279463-bli8hwda	205	9	.	.	.
cord-279463-bli8hwda	206	1	When	when	WRB
cord-279463-bli8hwda	206	2	the	the	DT
cord-279463-bli8hwda	206	3	membrane	membrane	NN
cord-279463-bli8hwda	206	4	-	-	HYPH
cord-279463-bli8hwda	206	5	spanning	span	VBG
cord-279463-bli8hwda	206	6	regions	region	NNS
cord-279463-bli8hwda	206	7	of	of	IN
cord-279463-bli8hwda	206	8	type	type	NN
cord-279463-bli8hwda	206	9	I	-PRON-	PRP
cord-279463-bli8hwda	206	10	and	and	CC
cord-279463-bli8hwda	206	11	type	type	NN
cord-279463-bli8hwda	206	12	II	ii	CD
cord-279463-bli8hwda	206	13	membrane	membrane	NN
cord-279463-bli8hwda	206	14	proteins	protein	NNS
cord-279463-bli8hwda	206	15	are	be	VBP
cord-279463-bli8hwda	206	16	compared	compare	VBN
cord-279463-bli8hwda	206	17	,	,	,
cord-279463-bli8hwda	206	18	no	no	DT
cord-279463-bli8hwda	206	19	obvious	obvious	JJ
cord-279463-bli8hwda	206	20	structural	structural	JJ
cord-279463-bli8hwda	206	21	difference	difference	NN
cord-279463-bli8hwda	206	22	can	can	MD
cord-279463-bli8hwda	206	23	be	be	VB
cord-279463-bli8hwda	206	24	found	find	VBN
cord-279463-bli8hwda	206	25	.	.	.
cord-279463-bli8hwda	207	1	In	in	IN
cord-279463-bli8hwda	207	2	both	both	DT
cord-279463-bli8hwda	207	3	types	type	NNS
cord-279463-bli8hwda	207	4	of	of	IN
cord-279463-bli8hwda	207	5	membrane	membrane	NN
cord-279463-bli8hwda	207	6	proteins	protein	NNS
cord-279463-bli8hwda	207	7	these	these	DT
cord-279463-bli8hwda	207	8	regions	region	NNS
cord-279463-bli8hwda	207	9	comprise	comprise	VBP
cord-279463-bli8hwda	207	10	a	a	DT
cord-279463-bli8hwda	207	11	stretch	stretch	NN
cord-279463-bli8hwda	207	12	of	of	IN
cord-279463-bli8hwda	207	13	20	20	CD
cord-279463-bli8hwda	207	14	to	to	TO
cord-279463-bli8hwda	207	15	30	30	CD
cord-279463-bli8hwda	207	16	hydrophobic	hydrophobic	JJ
cord-279463-bli8hwda	207	17	amino	amino	NN
cord-279463-bli8hwda	207	18	acid	acid	NN
cord-279463-bli8hwda	207	19	residues	residue	NNS
cord-279463-bli8hwda	207	20	that	that	WDT
cord-279463-bli8hwda	207	21	is	be	VBZ
cord-279463-bli8hwda	207	22	flanked	flank	VBN
cord-279463-bli8hwda	207	23	on	on	IN
cord-279463-bli8hwda	207	24	the	the	DT
cord-279463-bli8hwda	207	25	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	207	26	side	side	NN
cord-279463-bli8hwda	207	27	by	by	IN
cord-279463-bli8hwda	207	28	positively	positively	RB
cord-279463-bli8hwda	207	29	charged	charge	VBN
cord-279463-bli8hwda	207	30	amino	amino	NN
cord-279463-bli8hwda	207	31	acid	acid	NN
cord-279463-bli8hwda	207	32	residues	residue	NNS
cord-279463-bli8hwda	207	33	.	.	.
cord-279463-bli8hwda	208	1	In	in	IN
cord-279463-bli8hwda	208	2	type	type	NN
cord-279463-bli8hwda	208	3	I	-PRON-	PRP
cord-279463-bli8hwda	208	4	membrane	membrane	NN
cord-279463-bli8hwda	208	5	proteins	protein	NNS
cord-279463-bli8hwda	208	6	the	the	DT
cord-279463-bli8hwda	208	7	segment	segment	NN
cord-279463-bli8hwda	208	8	spanning	span	VBG
cord-279463-bli8hwda	208	9	the	the	DT
cord-279463-bli8hwda	208	10	membrane	membrane	NN
cord-279463-bli8hwda	208	11	does	do	VBZ
cord-279463-bli8hwda	208	12	not	not	RB
cord-279463-bli8hwda	208	13	appear	appear	VB
cord-279463-bli8hwda	208	14	to	to	TO
cord-279463-bli8hwda	208	15	participate	participate	VB
cord-279463-bli8hwda	208	16	in	in	IN
cord-279463-bli8hwda	208	17	the	the	DT
cord-279463-bli8hwda	208	18	initial	initial	JJ
cord-279463-bli8hwda	208	19	stage	stage	NN
cord-279463-bli8hwda	208	20	of	of	IN
cord-279463-bli8hwda	208	21	membrane	membrane	NN
cord-279463-bli8hwda	208	22	insertion	insertion	NN
cord-279463-bli8hwda	208	23	.	.	.
cord-279463-bli8hwda	209	1	Type	type	NN
cord-279463-bli8hwda	209	2	I	-PRON-	PRP
cord-279463-bli8hwda	209	3	membrane	membrane	NN
cord-279463-bli8hwda	209	4	proteins	protein	NNS
cord-279463-bli8hwda	209	5	usually	usually	RB
cord-279463-bli8hwda	209	6	have	have	VBP
cord-279463-bli8hwda	209	7	cleavable	cleavable	JJ
cord-279463-bli8hwda	209	8	signal	signal	NN
cord-279463-bli8hwda	209	9	sequences	sequence	NNS
cord-279463-bli8hwda	209	10	that	that	WDT
cord-279463-bli8hwda	209	11	initiate	initiate	VBP
cord-279463-bli8hwda	209	12	the	the	DT
cord-279463-bli8hwda	209	13	membrane	membrane	NN
cord-279463-bli8hwda	209	14	translocation	translocation	NN
cord-279463-bli8hwda	209	15	of	of	IN
cord-279463-bli8hwda	209	16	the	the	DT
cord-279463-bli8hwda	209	17	amino	amino	NN
cord-279463-bli8hwda	209	18	-	-	HYPH
cord-279463-bli8hwda	209	19	terminal	terminal	NN
cord-279463-bli8hwda	209	20	half	half	NN
cord-279463-bli8hwda	209	21	of	of	IN
cord-279463-bli8hwda	209	22	the	the	DT
cord-279463-bli8hwda	209	23	protein	protein	NN
cord-279463-bli8hwda	209	24	.	.	.
cord-279463-bli8hwda	210	1	The	the	DT
cord-279463-bli8hwda	210	2	membrane	membrane	NN
cord-279463-bli8hwda	210	3	-	-	HYPH
cord-279463-bli8hwda	210	4	spanning	span	VBG
cord-279463-bli8hwda	210	5	region	region	NN
cord-279463-bli8hwda	210	6	,	,	,
cord-279463-bli8hwda	210	7	in	in	IN
cord-279463-bli8hwda	210	8	its	-PRON-	PRP$
cord-279463-bli8hwda	210	9	position	position	NN
cord-279463-bli8hwda	210	10	close	close	RB
cord-279463-bli8hwda	210	11	to	to	IN
cord-279463-bli8hwda	210	12	the	the	DT
cord-279463-bli8hwda	210	13	carboxy	carboxy	JJ
cord-279463-bli8hwda	210	14	-	-	HYPH
cord-279463-bli8hwda	210	15	terminal	terminal	NN
cord-279463-bli8hwda	210	16	end	end	NN
cord-279463-bli8hwda	210	17	,	,	,
cord-279463-bli8hwda	210	18	seems	seem	VBZ
cord-279463-bli8hwda	210	19	only	only	RB
cord-279463-bli8hwda	210	20	to	to	TO
cord-279463-bli8hwda	210	21	function	function	VB
cord-279463-bli8hwda	210	22	in	in	IN
cord-279463-bli8hwda	210	23	anchoring	anchor	VBG
cord-279463-bli8hwda	210	24	the	the	DT
cord-279463-bli8hwda	210	25	protein	protein	NN
cord-279463-bli8hwda	210	26	in	in	IN
cord-279463-bli8hwda	210	27	the	the	DT
cord-279463-bli8hwda	210	28	membrane	membrane	NN
cord-279463-bli8hwda	210	29	.	.	.
cord-279463-bli8hwda	211	1	Yost	Yost	NNP
cord-279463-bli8hwda	211	2	et	et	NNP
cord-279463-bli8hwda	211	3	al	al	NNP
cord-279463-bli8hwda	211	4	.	.	.
cord-279463-bli8hwda	212	1	placed	place	VBD
cord-279463-bli8hwda	212	2	the	the	DT
cord-279463-bli8hwda	212	3	membrane	membrane	NN
cord-279463-bli8hwda	212	4	-	-	HYPH
cord-279463-bli8hwda	212	5	spanning	span	VBG
cord-279463-bli8hwda	212	6	segment	segment	NN
cord-279463-bli8hwda	212	7	of	of	IN
cord-279463-bli8hwda	212	8	the	the	DT
cord-279463-bli8hwda	212	9	murine	murine	JJ
cord-279463-bli8hwda	212	10	surface	surface	NN
cord-279463-bli8hwda	212	11	immunoglobulin	immunoglobulin	NN
cord-279463-bli8hwda	212	12	heavy	heavy	JJ
cord-279463-bli8hwda	212	13	chain	chain	NN
cord-279463-bli8hwda	212	14	close	close	RB
cord-279463-bli8hwda	212	15	to	to	IN
cord-279463-bli8hwda	212	16	the	the	DT
cord-279463-bli8hwda	212	17	amino	amino	NN
cord-279463-bli8hwda	212	18	-	-	HYPH
cord-279463-bli8hwda	212	19	terminal	terminal	NN
cord-279463-bli8hwda	212	20	end	end	NN
cord-279463-bli8hwda	212	21	of	of	IN
cord-279463-bli8hwda	212	22	a	a	DT
cord-279463-bli8hwda	212	23	fusion	fusion	NN
cord-279463-bli8hwda	212	24	protein	protein	NN
cord-279463-bli8hwda	212	25	(	(	-LRB-
cord-279463-bli8hwda	212	26	Yost	Yost	NNP
cord-279463-bli8hwda	212	27	et	et	NNP
cord-279463-bli8hwda	212	28	al	al	NNP
cord-279463-bli8hwda	212	29	.	.	NNP
cord-279463-bli8hwda	212	30	,	,	,
cord-279463-bli8hwda	212	31	1983	1983	CD
cord-279463-bli8hwda	212	32	)	)	-RRB-
cord-279463-bli8hwda	212	33	.	.	.
cord-279463-bli8hwda	213	1	In	in	IN
cord-279463-bli8hwda	213	2	this	this	DT
cord-279463-bli8hwda	213	3	position	position	NN
cord-279463-bli8hwda	213	4	the	the	DT
cord-279463-bli8hwda	213	5	segment	segment	NN
cord-279463-bli8hwda	213	6	did	do	VBD
cord-279463-bli8hwda	213	7	not	not	RB
cord-279463-bli8hwda	213	8	provide	provide	VB
cord-279463-bli8hwda	213	9	the	the	DT
cord-279463-bli8hwda	213	10	signal	signal	NN
cord-279463-bli8hwda	213	11	function	function	NN
cord-279463-bli8hwda	213	12	for	for	IN
cord-279463-bli8hwda	213	13	membrane	membrane	NN
cord-279463-bli8hwda	213	14	insertion	insertion	NN
cord-279463-bli8hwda	213	15	.	.	.
cord-279463-bli8hwda	214	1	As	as	IN
cord-279463-bli8hwda	214	2	,	,	,
cord-279463-bli8hwda	214	3	however	however	RB
cord-279463-bli8hwda	214	4	,	,	,
cord-279463-bli8hwda	214	5	a	a	DT
cord-279463-bli8hwda	214	6	hydrophilic	hydrophilic	JJ
cord-279463-bli8hwda	214	7	segment	segment	NN
cord-279463-bli8hwda	214	8	of	of	IN
cord-279463-bli8hwda	214	9	about	about	RB
cord-279463-bli8hwda	214	10	40	40	CD
cord-279463-bli8hwda	214	11	amino	amino	NN
cord-279463-bli8hwda	214	12	acid	acid	NN
cord-279463-bli8hwda	214	13	residues	residue	NNS
cord-279463-bli8hwda	214	14	precedes	precede	VBZ
cord-279463-bli8hwda	214	15	the	the	DT
cord-279463-bli8hwda	214	16	membrane	membrane	NN
cord-279463-bli8hwda	214	17	-	-	HYPH
cord-279463-bli8hwda	214	18	spanning	span	VBG
cord-279463-bli8hwda	214	19	segment	segment	NN
cord-279463-bli8hwda	214	20	,	,	,
cord-279463-bli8hwda	214	21	the	the	DT
cord-279463-bli8hwda	214	22	question	question	NN
cord-279463-bli8hwda	214	23	still	still	RB
cord-279463-bli8hwda	214	24	remains	remain	VBZ
cord-279463-bli8hwda	214	25	as	as	IN
cord-279463-bli8hwda	214	26	to	to	IN
cord-279463-bli8hwda	214	27	whether	whether	IN
cord-279463-bli8hwda	214	28	a	a	DT
cord-279463-bli8hwda	214	29	membrane	membrane	NN
cord-279463-bli8hwda	214	30	-	-	HYPH
cord-279463-bli8hwda	214	31	spanning	span	VBG
cord-279463-bli8hwda	214	32	region	region	NN
cord-279463-bli8hwda	214	33	from	from	IN
cord-279463-bli8hwda	214	34	a	a	DT
cord-279463-bli8hwda	214	35	type	type	NN
cord-279463-bli8hwda	214	36	I	-PRON-	PRP
cord-279463-bli8hwda	214	37	membrane	membrane	NN
cord-279463-bli8hwda	214	38	protein	protein	NN
cord-279463-bli8hwda	214	39	,	,	,
cord-279463-bli8hwda	214	40	when	when	WRB
cord-279463-bli8hwda	214	41	placed	place	VBN
cord-279463-bli8hwda	214	42	into	into	IN
cord-279463-bli8hwda	214	43	the	the	DT
cord-279463-bli8hwda	214	44	appropriate	appropriate	JJ
cord-279463-bli8hwda	214	45	surrounding	surrounding	NN
cord-279463-bli8hwda	214	46	,	,	,
cord-279463-bli8hwda	214	47	can	can	MD
cord-279463-bli8hwda	214	48	also	also	RB
cord-279463-bli8hwda	214	49	initiate	initiate	VB
cord-279463-bli8hwda	214	50	translocation	translocation	NN
cord-279463-bli8hwda	214	51	across	across	IN
cord-279463-bli8hwda	214	52	the	the	DT
cord-279463-bli8hwda	214	53	ER	ER	NNP
cord-279463-bli8hwda	214	54	membrane	membrane	NN
cord-279463-bli8hwda	214	55	.	.	.
cord-279463-bli8hwda	215	1	It	-PRON-	PRP
cord-279463-bli8hwda	215	2	is	be	VBZ
cord-279463-bli8hwda	215	3	well	well	RB
cord-279463-bli8hwda	215	4	conceivable	conceivable	JJ
cord-279463-bli8hwda	215	5	that	that	IN
cord-279463-bli8hwda	215	6	certain	certain	JJ
cord-279463-bli8hwda	215	7	hydrophilic	hydrophilic	JJ
cord-279463-bli8hwda	215	8	sequences	sequence	NNS
cord-279463-bli8hwda	215	9	preceding	precede	VBG
cord-279463-bli8hwda	215	10	a	a	DT
cord-279463-bli8hwda	215	11	hydrophobic	hydrophobic	JJ
cord-279463-bli8hwda	215	12	segment	segment	NN
cord-279463-bli8hwda	215	13	play	play	VB
cord-279463-bli8hwda	215	14	a	a	DT
cord-279463-bli8hwda	215	15	crucial	crucial	JJ
cord-279463-bli8hwda	215	16	role	role	NN
cord-279463-bli8hwda	215	17	in	in	IN
cord-279463-bli8hwda	215	18	exposing	expose	VBG
cord-279463-bli8hwda	215	19	a	a	DT
cord-279463-bli8hwda	215	20	potential	potential	JJ
cord-279463-bli8hwda	215	21	signal	signal	NN
cord-279463-bli8hwda	215	22	for	for	IN
cord-279463-bli8hwda	215	23	membrane	membrane	NN
cord-279463-bli8hwda	215	24	insertion	insertion	NN
cord-279463-bli8hwda	215	25	.	.	.
cord-279463-bli8hwda	216	1	Up	up	IN
cord-279463-bli8hwda	216	2	to	to	IN
cord-279463-bli8hwda	216	3	now	now	RB
cord-279463-bli8hwda	216	4	no	no	DT
cord-279463-bli8hwda	216	5	special	special	JJ
cord-279463-bli8hwda	216	6	structural	structural	JJ
cord-279463-bli8hwda	216	7	features	feature	NNS
cord-279463-bli8hwda	216	8	,	,	,
cord-279463-bli8hwda	216	9	besides	besides	IN
cord-279463-bli8hwda	216	10	hydrophobicity	hydrophobicity	NN
cord-279463-bli8hwda	216	11	,	,	,
cord-279463-bli8hwda	216	12	are	be	VBP
cord-279463-bli8hwda	216	13	known	know	VBN
cord-279463-bli8hwda	216	14	to	to	TO
cord-279463-bli8hwda	216	15	be	be	VB
cord-279463-bli8hwda	216	16	crucial	crucial	JJ
cord-279463-bli8hwda	216	17	for	for	IN
cord-279463-bli8hwda	216	18	the	the	DT
cord-279463-bli8hwda	216	19	function	function	NN
cord-279463-bli8hwda	216	20	of	of	IN
cord-279463-bli8hwda	216	21	a	a	DT
cord-279463-bli8hwda	216	22	signal	signal	JJ
cord-279463-bli8hwda	216	23	sequence	sequence	NN
cord-279463-bli8hwda	216	24	.	.	.
cord-279463-bli8hwda	217	1	A	a	DT
cord-279463-bli8hwda	217	2	common	common	JJ
cord-279463-bli8hwda	217	3	step	step	NN
cord-279463-bli8hwda	217	4	has	have	VBZ
cord-279463-bli8hwda	217	5	been	be	VBN
cord-279463-bli8hwda	217	6	proposed	propose	VBN
cord-279463-bli8hwda	217	7	for	for	IN
cord-279463-bli8hwda	217	8	the	the	DT
cord-279463-bli8hwda	217	9	early	early	JJ
cord-279463-bli8hwda	217	10	stage	stage	NN
cord-279463-bli8hwda	217	11	of	of	IN
cord-279463-bli8hwda	217	12	membrane	membrane	NN
cord-279463-bli8hwda	217	13	insertion	insertion	NN
cord-279463-bli8hwda	217	14	of	of	IN
cord-279463-bli8hwda	217	15	secretory	secretory	NN
cord-279463-bli8hwda	217	16	and	and	CC
cord-279463-bli8hwda	217	17	membrane	membrane	NN
cord-279463-bli8hwda	217	18	proteins	protein	NNS
cord-279463-bli8hwda	218	1	(	(	-LRB-
cord-279463-bli8hwda	218	2	Dobberstein	Dobberstein	NNP
cord-279463-bli8hwda	218	3	et	et	NNP
cord-279463-bli8hwda	218	4	al	al	NNP
cord-279463-bli8hwda	218	5	.	.	.
cord-279463-bli8hwda	219	1	,	,	,
cord-279463-bli8hwda	219	2	1983	1983	CD
cord-279463-bli8hwda	219	3	;	;	:
cord-279463-bli8hwda	219	4	Spiess	Spiess	NNP
cord-279463-bli8hwda	219	5	and	and	CC
cord-279463-bli8hwda	219	6	Lodish	Lodish	NNP
cord-279463-bli8hwda	219	7	,	,	,
cord-279463-bli8hwda	219	8	1986	1986	CD
cord-279463-bli8hwda	219	9	;	;	:
cord-279463-bli8hwda	219	10	Lipp	Lipp	NNP
cord-279463-bli8hwda	219	11	and	and	CC
cord-279463-bli8hwda	219	12	Dobberstein	Dobberstein	NNP
cord-279463-bli8hwda	219	13	,	,	,
cord-279463-bli8hwda	219	14	1986	1986	CD
cord-279463-bli8hwda	219	15	)	)	-RRB-
cord-279463-bli8hwda	219	16	.	.	.
cord-279463-bli8hwda	220	1	This	this	DT
cord-279463-bli8hwda	220	2	was	be	VBD
cord-279463-bli8hwda	220	3	based	base	VBN
cord-279463-bli8hwda	220	4	largely	largely	RB
cord-279463-bli8hwda	220	5	on	on	IN
cord-279463-bli8hwda	220	6	the	the	DT
cord-279463-bli8hwda	220	7	finding	finding	NN
cord-279463-bli8hwda	220	8	that	that	IN
cord-279463-bli8hwda	220	9	both	both	DT
cord-279463-bli8hwda	220	10	of	of	IN
cord-279463-bli8hwda	220	11	these	these	DT
cord-279463-bli8hwda	220	12	types	type	NNS
cord-279463-bli8hwda	220	13	of	of	IN
cord-279463-bli8hwda	220	14	proteins	protein	NNS
cord-279463-bli8hwda	220	15	require	require	VBP
cord-279463-bli8hwda	220	16	SRP	SRP	NNP
cord-279463-bli8hwda	220	17	and	and	CC
cord-279463-bli8hwda	220	18	docking	docking	NN
cord-279463-bli8hwda	220	19	protein	protein	NN
cord-279463-bli8hwda	220	20	for	for	IN
cord-279463-bli8hwda	220	21	their	-PRON-	PRP$
cord-279463-bli8hwda	220	22	membrane	membrane	NN
cord-279463-bli8hwda	220	23	insertion	insertion	NN
cord-279463-bli8hwda	220	24	.	.	.
cord-279463-bli8hwda	221	1	Here	here	RB
cord-279463-bli8hwda	221	2	,	,	,
cord-279463-bli8hwda	221	3	we	-PRON-	PRP
cord-279463-bli8hwda	221	4	show	show	VBP
cord-279463-bli8hwda	221	5	that	that	IN
cord-279463-bli8hwda	221	6	a	a	DT
cord-279463-bli8hwda	221	7	type	type	NN
cord-279463-bli8hwda	221	8	II	II	NNP
cord-279463-bli8hwda	221	9	membrane	membrane	NN
cord-279463-bli8hwda	221	10	protein	protein	NN
cord-279463-bli8hwda	221	11	can	can	MD
cord-279463-bli8hwda	221	12	be	be	VB
cord-279463-bli8hwda	221	13	converted	convert	VBN
cord-279463-bli8hwda	221	14	into	into	IN
cord-279463-bli8hwda	221	15	a	a	DT
cord-279463-bli8hwda	221	16	secretory	secretory	JJ
cord-279463-bli8hwda	221	17	protein	protein	NN
cord-279463-bli8hwda	221	18	by	by	IN
cord-279463-bli8hwda	221	19	removal	removal	NN
cord-279463-bli8hwda	221	20	of	of	IN
cord-279463-bli8hwda	221	21	the	the	DT
cord-279463-bli8hwda	221	22	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	221	23	segment	segment	NN
cord-279463-bli8hwda	221	24	.	.	.
cord-279463-bli8hwda	222	1	This	this	DT
cord-279463-bli8hwda	222	2	directly	directly	RB
cord-279463-bli8hwda	222	3	demonstrates	demonstrate	VBZ
cord-279463-bli8hwda	222	4	that	that	IN
cord-279463-bli8hwda	222	5	the	the	DT
cord-279463-bli8hwda	222	6	signal	signal	NN
cord-279463-bli8hwda	222	7	for	for	IN
cord-279463-bli8hwda	222	8	membrane	membrane	NN
cord-279463-bli8hwda	222	9	insertion	insertion	NN
cord-279463-bli8hwda	222	10	of	of	IN
cord-279463-bli8hwda	222	11	these	these	DT
cord-279463-bli8hwda	222	12	two	two	CD
cord-279463-bli8hwda	222	13	types	type	NNS
cord-279463-bli8hwda	222	14	of	of	IN
cord-279463-bli8hwda	222	15	proteins	protein	NNS
cord-279463-bli8hwda	222	16	can	can	MD
cord-279463-bli8hwda	222	17	be	be	VB
cord-279463-bli8hwda	222	18	the	the	DT
cord-279463-bli8hwda	222	19	same	same	JJ
cord-279463-bli8hwda	222	20	.	.	.
cord-279463-bli8hwda	223	1	Further	further	JJ
cord-279463-bli8hwda	223	2	deletion	deletion	NN
cord-279463-bli8hwda	223	3	into	into	IN
cord-279463-bli8hwda	223	4	the	the	DT
cord-279463-bli8hwda	223	5	carboxy	carboxy	JJ
cord-279463-bli8hwda	223	6	-	-	HYPH
cord-279463-bli8hwda	223	7	terminal	terminal	NN
cord-279463-bli8hwda	223	8	half	half	NN
cord-279463-bli8hwda	223	9	of	of	IN
cord-279463-bli8hwda	223	10	the	the	DT
cord-279463-bli8hwda	223	11	ly	ly	NNP
cord-279463-bli8hwda	223	12	hydrophobic	hydrophobic	JJ
cord-279463-bli8hwda	223	13	segment	segment	NN
cord-279463-bli8hwda	223	14	is	be	VBZ
cord-279463-bli8hwda	223	15	required	require	VBN
cord-279463-bli8hwda	223	16	to	to	TO
cord-279463-bli8hwda	223	17	elucidate	elucidate	VB
cord-279463-bli8hwda	223	18	whether	whether	IN
cord-279463-bli8hwda	223	19	the	the	DT
cord-279463-bli8hwda	223	20	cleaved	cleaved	JJ
cord-279463-bli8hwda	223	21	signal	signal	NN
cord-279463-bli8hwda	223	22	sequence	sequence	NN
cord-279463-bli8hwda	223	23	contains	contain	VBZ
cord-279463-bli8hwda	223	24	all	all	PDT
cord-279463-bli8hwda	223	25	the	the	DT
cord-279463-bli8hwda	223	26	information	information	NN
cord-279463-bli8hwda	223	27	for	for	IN
cord-279463-bli8hwda	223	28	membrane	membrane	NN
cord-279463-bli8hwda	223	29	insertion	insertion	NN
cord-279463-bli8hwda	223	30	.	.	.
cord-279463-bli8hwda	224	1	It	-PRON-	PRP
cord-279463-bli8hwda	224	2	is	be	VBZ
cord-279463-bli8hwda	224	3	conceivable	conceivable	JJ
cord-279463-bli8hwda	224	4	that	that	IN
cord-279463-bli8hwda	224	5	the	the	DT
cord-279463-bli8hwda	224	6	functional	functional	JJ
cord-279463-bli8hwda	224	7	signal	signal	NN
cord-279463-bli8hwda	224	8	sequence	sequence	NN
cord-279463-bli8hwda	224	9	extends	extend	VBZ
cord-279463-bli8hwda	224	10	over	over	IN
cord-279463-bli8hwda	224	11	the	the	DT
cord-279463-bli8hwda	224	12	cleaved	cleave	VBN
cord-279463-bli8hwda	224	13	signal	signal	NN
cord-279463-bli8hwda	224	14	sequence	sequence	NN
cord-279463-bli8hwda	224	15	into	into	IN
cord-279463-bli8hwda	224	16	the	the	DT
cord-279463-bli8hwda	224	17	adjacent	adjacent	JJ
cord-279463-bli8hwda	224	18	hydrophobic	hydrophobic	JJ
cord-279463-bli8hwda	224	19	part	part	NN
cord-279463-bli8hwda	224	20	.	.	.
cord-279463-bli8hwda	225	1	For	for	IN
cord-279463-bli8hwda	225	2	some	some	DT
cord-279463-bli8hwda	225	3	secretory	secretory	JJ
cord-279463-bli8hwda	225	4	protein	protein	NN
cord-279463-bli8hwda	225	5	it	-PRON-	PRP
cord-279463-bli8hwda	225	6	has	have	VBZ
cord-279463-bli8hwda	225	7	been	be	VBN
cord-279463-bli8hwda	225	8	observed	observe	VBN
cord-279463-bli8hwda	225	9	that	that	IN
cord-279463-bli8hwda	225	10	the	the	DT
cord-279463-bli8hwda	225	11	cleavable	cleavable	JJ
cord-279463-bli8hwda	225	12	signal	signal	NN
cord-279463-bli8hwda	225	13	sequence	sequence	NN
cord-279463-bli8hwda	225	14	is	be	VBZ
cord-279463-bli8hwda	225	15	not	not	RB
cord-279463-bli8hwda	225	16	sufficient	sufficient	JJ
cord-279463-bli8hwda	225	17	for	for	IN
cord-279463-bli8hwda	225	18	membrane	membrane	NN
cord-279463-bli8hwda	225	19	insertion	insertion	NN
cord-279463-bli8hwda	225	20	.	.	.
cord-279463-bli8hwda	226	1	In	in	IN
cord-279463-bli8hwda	226	2	the	the	DT
cord-279463-bli8hwda	226	3	case	case	NN
cord-279463-bli8hwda	226	4	of	of	IN
cord-279463-bli8hwda	226	5	staphylococcal	staphylococcal	JJ
cord-279463-bli8hwda	226	6	protein	protein	NN
cord-279463-bli8hwda	226	7	A	A	NNP
cord-279463-bli8hwda	226	8	,	,	,
cord-279463-bli8hwda	226	9	sequences	sequence	NNS
cord-279463-bli8hwda	226	10	of	of	IN
cord-279463-bli8hwda	226	11	the	the	DT
cord-279463-bli8hwda	226	12	amino	amino	NN
cord-279463-bli8hwda	226	13	-	-	HYPH
cord-279463-bli8hwda	226	14	terminal	terminal	NN
cord-279463-bli8hwda	226	15	part	part	NN
cord-279463-bli8hwda	226	16	of	of	IN
cord-279463-bli8hwda	226	17	the	the	DT
cord-279463-bli8hwda	226	18	mature	mature	JJ
cord-279463-bli8hwda	226	19	protein	protein	NN
cord-279463-bli8hwda	226	20	are	be	VBP
cord-279463-bli8hwda	226	21	required	require	VBN
cord-279463-bli8hwda	226	22	for	for	IN
cord-279463-bli8hwda	226	23	membrane	membrane	NN
cord-279463-bli8hwda	226	24	insertion	insertion	NN
cord-279463-bli8hwda	226	25	and	and	CC
cord-279463-bli8hwda	226	26	correct	correct	JJ
cord-279463-bli8hwda	226	27	processing	processing	NN
cord-279463-bli8hwda	226	28	(	(	-LRB-
cord-279463-bli8hwda	226	29	Abrahmsen	Abrahmsen	NNP
cord-279463-bli8hwda	226	30	et	et	NNP
cord-279463-bli8hwda	226	31	al	al	NNP
cord-279463-bli8hwda	226	32	.	.	NNP
cord-279463-bli8hwda	226	33	,	,	,
cord-279463-bli8hwda	226	34	1985	1985	CD
cord-279463-bli8hwda	226	35	)	)	-RRB-
cord-279463-bli8hwda	226	36	.	.	.
cord-279463-bli8hwda	227	1	SRP	SRP	NNP
cord-279463-bli8hwda	227	2	can	can	MD
cord-279463-bli8hwda	227	3	arrest	arrest	VB
cord-279463-bli8hwda	227	4	elongation	elongation	NN
cord-279463-bli8hwda	227	5	of	of	IN
cord-279463-bli8hwda	227	6	presecretory	presecretory	NN
cord-279463-bli8hwda	227	7	and	and	CC
cord-279463-bli8hwda	227	8	type	type	NN
cord-279463-bli8hwda	227	9	II	II	NNP
cord-279463-bli8hwda	227	10	membrane	membrane	NN
cord-279463-bli8hwda	227	11	proteins	protein	NNS
cord-279463-bli8hwda	227	12	after	after	IN
cord-279463-bli8hwda	227	13	70	70	CD
cord-279463-bli8hwda	227	14	or	or	CC
cord-279463-bli8hwda	227	15	even	even	RB
cord-279463-bli8hwda	227	16	more	more	JJR
cord-279463-bli8hwda	227	17	amino	amino	NN
cord-279463-bli8hwda	227	18	acid	acid	NN
cord-279463-bli8hwda	227	19	residues	residue	NNS
cord-279463-bli8hwda	227	20	have	have	VBP
cord-279463-bli8hwda	227	21	been	be	VBN
cord-279463-bli8hwda	227	22	polymerized	polymerize	VBN
cord-279463-bli8hwda	227	23	(	(	-LRB-
cord-279463-bli8hwda	227	24	Walter	Walter	NNP
cord-279463-bli8hwda	227	25	and	and	CC
cord-279463-bli8hwda	227	26	Blobel	Blobel	NNP
cord-279463-bli8hwda	227	27	,	,	,
cord-279463-bli8hwda	227	28	1981a	1981a	CD
cord-279463-bli8hwda	227	29	;	;	:
cord-279463-bli8hwda	228	1	Meyer	Meyer	NNP
cord-279463-bli8hwda	228	2	et	et	NNP
cord-279463-bli8hwda	228	3	al	al	NNP
cord-279463-bli8hwda	228	4	.	.	.
cord-279463-bli8hwda	229	1	,	,	,
cord-279463-bli8hwda	229	2	1982	1982	CD
cord-279463-bli8hwda	229	3	;	;	:
cord-279463-bli8hwda	229	4	Lipp	Lipp	NNP
cord-279463-bli8hwda	229	5	and	and	CC
cord-279463-bli8hwda	229	6	Dobberstein	Dobberstein	NNP
cord-279463-bli8hwda	229	7	,	,	,
cord-279463-bli8hwda	229	8	1986	1986	CD
cord-279463-bli8hwda	229	9	;	;	:
cord-279463-bli8hwda	229	10	Lipp	Lipp	NNP
cord-279463-bli8hwda	229	11	et	et	NNP
cord-279463-bli8hwda	229	12	al	al	NNP
cord-279463-bli8hwda	229	13	.	.	NNP
cord-279463-bli8hwda	229	14	,	,	,
cord-279463-bli8hwda	229	15	unpublished	unpublished	JJ
cord-279463-bli8hwda	229	16	data	datum	NNS
cord-279463-bli8hwda	229	17	)	)	-RRB-
cord-279463-bli8hwda	229	18	.	.	.
cord-279463-bli8hwda	230	1	These	these	DT
cord-279463-bli8hwda	230	2	domains	domain	NNS
cord-279463-bli8hwda	230	3	are	be	VBP
cord-279463-bli8hwda	230	4	then	then	RB
cord-279463-bli8hwda	230	5	inserted	insert	VBN
cord-279463-bli8hwda	230	6	into	into	IN
cord-279463-bli8hwda	230	7	the	the	DT
cord-279463-bli8hwda	230	8	ER	ER	NNP
cord-279463-bli8hwda	230	9	membrane	membrane	NN
cord-279463-bli8hwda	230	10	by	by	IN
cord-279463-bli8hwda	230	11	a	a	DT
cord-279463-bli8hwda	230	12	yet	yet	RB
cord-279463-bli8hwda	230	13	unknown	unknown	JJ
cord-279463-bli8hwda	230	14	mechanism	mechanism	NN
cord-279463-bli8hwda	230	15	.	.	.
cord-279463-bli8hwda	231	1	As	as	IN
cord-279463-bli8hwda	231	2	the	the	DT
cord-279463-bli8hwda	231	3	amino	amino	NN
cord-279463-bli8hwda	231	4	terminus	terminus	NN
cord-279463-bli8hwda	231	5	of	of	IN
cord-279463-bli8hwda	231	6	a	a	DT
cord-279463-bli8hwda	231	7	type	type	NN
cord-279463-bli8hwda	231	8	II	II	NNP
cord-279463-bli8hwda	231	9	membrane	membrane	NN
cord-279463-bli8hwda	231	10	protein	protein	NN
cord-279463-bli8hwda	231	11	has	have	VBZ
cord-279463-bli8hwda	231	12	to	to	TO
cord-279463-bli8hwda	231	13	remain	remain	VB
cord-279463-bli8hwda	231	14	on	on	IN
cord-279463-bli8hwda	231	15	the	the	DT
cord-279463-bli8hwda	231	16	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	231	17	side	side	NN
cord-279463-bli8hwda	231	18	,	,	,
cord-279463-bli8hwda	231	19	the	the	DT
cord-279463-bli8hwda	231	20	formation	formation	NN
cord-279463-bli8hwda	231	21	of	of	IN
cord-279463-bli8hwda	231	22	a	a	DT
cord-279463-bli8hwda	231	23	loop	loop	NN
cord-279463-bli8hwda	231	24	during	during	IN
cord-279463-bli8hwda	231	25	membrane	membrane	NN
cord-279463-bli8hwda	231	26	insertion	insertion	NN
cord-279463-bli8hwda	231	27	has	have	VBZ
cord-279463-bli8hwda	231	28	been	be	VBN
cord-279463-bli8hwda	231	29	proposed	propose	VBN
cord-279463-bli8hwda	231	30	.	.	.
cord-279463-bli8hwda	232	1	In	in	IN
cord-279463-bli8hwda	232	2	the	the	DT
cord-279463-bli8hwda	232	3	case	case	NN
cord-279463-bli8hwda	232	4	of	of	IN
cord-279463-bli8hwda	232	5	a	a	DT
cord-279463-bli8hwda	232	6	secretory	secretory	JJ
cord-279463-bli8hwda	232	7	protein	protein	NN
cord-279463-bli8hwda	232	8	,	,	,
cord-279463-bli8hwda	232	9	signal	signal	NN
cord-279463-bli8hwda	232	10	peptidase	peptidase	NN
cord-279463-bli8hwda	232	11	would	would	MD
cord-279463-bli8hwda	232	12	be	be	VB
cord-279463-bli8hwda	232	13	able	able	JJ
cord-279463-bli8hwda	232	14	to	to	TO
cord-279463-bli8hwda	232	15	act	act	VB
cord-279463-bli8hwda	232	16	as	as	RB
cord-279463-bli8hwda	232	17	soon	soon	RB
cord-279463-bli8hwda	232	18	as	as	IN
cord-279463-bli8hwda	232	19	the	the	DT
cord-279463-bli8hwda	232	20	loop	loop	NN
cord-279463-bli8hwda	232	21	appears	appear	VBZ
cord-279463-bli8hwda	232	22	on	on	IN
cord-279463-bli8hwda	232	23	the	the	DT
cord-279463-bli8hwda	232	24	exoplasmic	exoplasmic	JJ
cord-279463-bli8hwda	232	25	side	side	NN
cord-279463-bli8hwda	232	26	.	.	.
cord-279463-bli8hwda	233	1	An	an	DT
cord-279463-bli8hwda	233	2	initial	initial	JJ
cord-279463-bli8hwda	233	3	interaction	interaction	NN
cord-279463-bli8hwda	233	4	of	of	IN
cord-279463-bli8hwda	233	5	basic	basic	JJ
cord-279463-bli8hwda	233	6	residues	residue	NNS
cord-279463-bli8hwda	233	7	in	in	IN
cord-279463-bli8hwda	233	8	a	a	DT
cord-279463-bli8hwda	233	9	signal	signal	JJ
cord-279463-bli8hwda	233	10	sequence	sequence	NN
cord-279463-bli8hwda	233	11	with	with	IN
cord-279463-bli8hwda	233	12	the	the	DT
cord-279463-bli8hwda	233	13	phosphates	phosphate	NNS
cord-279463-bli8hwda	233	14	of	of	IN
cord-279463-bli8hwda	233	15	the	the	DT
cord-279463-bli8hwda	233	16	membrane	membrane	NN
cord-279463-bli8hwda	233	17	lipids	lipid	NNS
cord-279463-bli8hwda	233	18	was	be	VBD
cord-279463-bli8hwda	233	19	originally	originally	RB
cord-279463-bli8hwda	233	20	proposed	propose	VBN
cord-279463-bli8hwda	233	21	by	by	IN
cord-279463-bli8hwda	233	22	lnouye	lnouye	NNP
cord-279463-bli8hwda	233	23	for	for	IN
cord-279463-bli8hwda	233	24	the	the	DT
cord-279463-bli8hwda	233	25	lipoprotein	lipoprotein	NN
cord-279463-bli8hwda	233	26	of	of	IN
cord-279463-bli8hwda	233	27	E.	E.	NNP
cord-279463-bli8hwda	233	28	coli	coli	NNS
cord-279463-bli8hwda	233	29	(	(	-LRB-
cord-279463-bli8hwda	233	30	Inouye	Inouye	NNP
cord-279463-bli8hwda	233	31	et	et	NNP
cord-279463-bli8hwda	233	32	al	al	NNP
cord-279463-bli8hwda	233	33	.	.	NNP
cord-279463-bli8hwda	233	34	,	,	,
cord-279463-bli8hwda	233	35	1977	1977	CD
cord-279463-bli8hwda	233	36	)	)	-RRB-
cord-279463-bli8hwda	233	37	.	.	.
cord-279463-bli8hwda	234	1	Our	-PRON-	PRP$
cord-279463-bli8hwda	234	2	results	result	NNS
cord-279463-bli8hwda	234	3	rule	rule	VBP
cord-279463-bli8hwda	234	4	out	out	RP
cord-279463-bli8hwda	234	5	an	an	DT
cord-279463-bli8hwda	234	6	essential	essential	JJ
cord-279463-bli8hwda	234	7	role	role	NN
cord-279463-bli8hwda	234	8	of	of	IN
cord-279463-bli8hwda	234	9	these	these	DT
cord-279463-bli8hwda	234	10	basic	basic	JJ
cord-279463-bli8hwda	234	11	residues	residue	NNS
cord-279463-bli8hwda	234	12	in	in	IN
cord-279463-bli8hwda	234	13	ER	ER	NNP
cord-279463-bli8hwda	234	14	membrane	membrane	NN
cord-279463-bli8hwda	234	15	insertion	insertion	NN
cord-279463-bli8hwda	234	16	.	.	.
cord-279463-bli8hwda	235	1	The	the	DT
cord-279463-bli8hwda	235	2	AN	AN	NNP
cord-279463-bli8hwda	235	3	-	-	HYPH
cord-279463-bli8hwda	235	4	IyCAT	IyCAT	NNP
cord-279463-bli8hwda	235	5	protein	protein	NN
cord-279463-bli8hwda	235	6	does	do	VBZ
cord-279463-bli8hwda	235	7	not	not	RB
cord-279463-bli8hwda	235	8	contain	contain	VB
cord-279463-bli8hwda	235	9	any	any	DT
cord-279463-bli8hwda	235	10	charged	charge	VBN
cord-279463-bli8hwda	235	11	amino	amino	NN
cord-279463-bli8hwda	235	12	acid	acid	NN
cord-279463-bli8hwda	235	13	residues	residue	NNS
cord-279463-bli8hwda	235	14	preceding	precede	VBG
cord-279463-bli8hwda	235	15	the	the	DT
cord-279463-bli8hwda	235	16	hydrophobic	hydrophobic	JJ
cord-279463-bli8hwda	235	17	segment	segment	NN
cord-279463-bli8hwda	235	18	.	.	.
cord-279463-bli8hwda	236	1	It	-PRON-	PRP
cord-279463-bli8hwda	236	2	is	be	VBZ
cord-279463-bli8hwda	236	3	nevertheless	nevertheless	RB
cord-279463-bli8hwda	236	4	translocated	translocate	VBN
cord-279463-bli8hwda	236	5	across	across	IN
cord-279463-bli8hwda	236	6	the	the	DT
cord-279463-bli8hwda	236	7	ER	ER	NNP
cord-279463-bli8hwda	236	8	membrane	membrane	NN
cord-279463-bli8hwda	236	9	and	and	CC
cord-279463-bli8hwda	236	10	processed	process	VBN
cord-279463-bli8hwda	236	11	.	.	.
cord-279463-bli8hwda	237	1	The	the	DT
cord-279463-bli8hwda	237	2	rules	rule	NNS
cord-279463-bli8hwda	237	3	that	that	WDT
cord-279463-bli8hwda	237	4	define	define	VBP
cord-279463-bli8hwda	237	5	the	the	DT
cord-279463-bli8hwda	237	6	cleavage	cleavage	NN
cord-279463-bli8hwda	237	7	site	site	NN
cord-279463-bli8hwda	237	8	for	for	IN
cord-279463-bli8hwda	237	9	signal	signal	NN
cord-279463-bli8hwda	237	10	peptidase	peptidase	NN
cord-279463-bli8hwda	237	11	in	in	IN
cord-279463-bli8hwda	237	12	presecretory	presecretory	NN
cord-279463-bli8hwda	237	13	proteins	protein	NNS
cord-279463-bli8hwda	237	14	are	be	VBP
cord-279463-bli8hwda	237	15	not	not	RB
cord-279463-bli8hwda	237	16	yet	yet	RB
cord-279463-bli8hwda	237	17	fully	fully	RB
cord-279463-bli8hwda	237	18	understood	understand	VBN
cord-279463-bli8hwda	237	19	.	.	.
cord-279463-bli8hwda	238	1	Von	Von	NNP
cord-279463-bli8hwda	238	2	Heijne	Heijne	NNP
cord-279463-bli8hwda	238	3	points	point	VBZ
cord-279463-bli8hwda	238	4	out	out	RP
cord-279463-bli8hwda	238	5	that	that	IN
cord-279463-bli8hwda	238	6	the	the	DT
cord-279463-bli8hwda	238	7	type	type	NN
cord-279463-bli8hwda	238	8	of	of	IN
cord-279463-bli8hwda	238	9	amino	amino	JJ
cord-279463-bli8hwda	238	10	acids	acid	NNS
cord-279463-bli8hwda	238	11	at	at	IN
cord-279463-bli8hwda	238	12	the	the	DT
cord-279463-bli8hwda	238	13	-1	-1	NN
cord-279463-bli8hwda	238	14	and	and	CC
cord-279463-bli8hwda	238	15	-3	-3	NNP
cord-279463-bli8hwda	238	16	position	position	NN
cord-279463-bli8hwda	238	17	in	in	IN
cord-279463-bli8hwda	238	18	front	front	NN
cord-279463-bli8hwda	238	19	of	of	IN
cord-279463-bli8hwda	238	20	the	the	DT
cord-279463-bli8hwda	238	21	site	site	NN
cord-279463-bli8hwda	238	22	of	of	IN
cord-279463-bli8hwda	238	23	cleavage	cleavage	NN
cord-279463-bli8hwda	238	24	are	be	VBP
cord-279463-bli8hwda	238	25	im	im	RB
cord-279463-bli8hwda	238	26	-	-	HYPH
cord-279463-bli8hwda	238	27	portant	portant	JJ
cord-279463-bli8hwda	238	28	in	in	IN
cord-279463-bli8hwda	238	29	assigning	assign	VBG
cord-279463-bli8hwda	238	30	a	a	DT
cord-279463-bli8hwda	238	31	cleavage	cleavage	NN
cord-279463-bli8hwda	238	32	site	site	NN
cord-279463-bli8hwda	238	33	.	.	.
cord-279463-bli8hwda	239	1	Here	here	RB
cord-279463-bli8hwda	239	2	we	-PRON-	PRP
cord-279463-bli8hwda	239	3	show	show	VBP
cord-279463-bli8hwda	239	4	that	that	IN
cord-279463-bli8hwda	239	5	sequences	sequence	NNS
cord-279463-bli8hwda	239	6	at	at	IN
cord-279463-bli8hwda	239	7	the	the	DT
cord-279463-bli8hwda	239	8	very	very	JJ
cord-279463-bli8hwda	239	9	beginning	beginning	NN
cord-279463-bli8hwda	239	10	of	of	IN
cord-279463-bli8hwda	239	11	a	a	DT
cord-279463-bli8hwda	239	12	signal	signal	JJ
cord-279463-bli8hwda	239	13	sequence	sequence	NN
cord-279463-bli8hwda	239	14	can	can	MD
cord-279463-bli8hwda	239	15	also	also	RB
cord-279463-bli8hwda	239	16	influence	influence	VB
cord-279463-bli8hwda	239	17	cleavage	cleavage	NN
cord-279463-bli8hwda	239	18	by	by	IN
cord-279463-bli8hwda	239	19	signal	signal	NN
cord-279463-bli8hwda	239	20	peptidase	peptidase	NN
cord-279463-bli8hwda	239	21	.	.	.
cord-279463-bli8hwda	240	1	In	in	IN
cord-279463-bli8hwda	240	2	the	the	DT
cord-279463-bli8hwda	240	3	case	case	NN
cord-279463-bli8hwda	240	4	of	of	IN
cord-279463-bli8hwda	240	5	ly	ly	XX
cord-279463-bli8hwda	240	6	,	,	,
cord-279463-bli8hwda	240	7	these	these	DT
cord-279463-bli8hwda	240	8	charged	charge	VBN
cord-279463-bli8hwda	240	9	residues	residue	NNS
cord-279463-bli8hwda	240	10	can	can	MD
cord-279463-bli8hwda	240	11	prevent	prevent	VB
cord-279463-bli8hwda	240	12	cleavage	cleavage	NN
cord-279463-bli8hwda	240	13	by	by	IN
cord-279463-bli8hwda	240	14	signal	signal	NN
cord-279463-bli8hwda	240	15	peptidase	peptidase	NN
cord-279463-bli8hwda	240	16	.	.	.
cord-279463-bli8hwda	241	1	The	the	DT
cord-279463-bli8hwda	241	2	variability	variability	NN
cord-279463-bli8hwda	241	3	in	in	IN
cord-279463-bli8hwda	241	4	the	the	DT
cord-279463-bli8hwda	241	5	length	length	NN
cord-279463-bli8hwda	241	6	and	and	CC
cord-279463-bli8hwda	241	7	in	in	IN
cord-279463-bli8hwda	241	8	the	the	DT
cord-279463-bli8hwda	241	9	amount	amount	NN
cord-279463-bli8hwda	241	10	of	of	IN
cord-279463-bli8hwda	241	11	charged	charge	VBN
cord-279463-bli8hwda	241	12	amino	amino	NN
cord-279463-bli8hwda	241	13	acid	acid	NN
cord-279463-bli8hwda	241	14	residues	residue	NNS
cord-279463-bli8hwda	241	15	at	at	IN
cord-279463-bli8hwda	241	16	the	the	DT
cord-279463-bli8hwda	241	17	amino	amino	NN
cord-279463-bli8hwda	241	18	terminus	terminus	NN
cord-279463-bli8hwda	241	19	of	of	IN
cord-279463-bli8hwda	241	20	asignal	asignal	JJ
cord-279463-bli8hwda	241	21	sequence	sequence	NN
cord-279463-bli8hwda	241	22	has	have	VBZ
cord-279463-bli8hwda	241	23	not	not	RB
cord-279463-bli8hwda	241	24	as	as	RB
cord-279463-bli8hwda	241	25	yet	yet	RB
cord-279463-bli8hwda	241	26	been	be	VBN
cord-279463-bli8hwda	241	27	explained	explain	VBN
cord-279463-bli8hwda	241	28	.	.	.
cord-279463-bli8hwda	242	1	Mutation	mutation	NN
cord-279463-bli8hwda	242	2	and	and	CC
cord-279463-bli8hwda	242	3	deletion	deletion	NN
cord-279463-bli8hwda	242	4	experiments	experiment	NNS
cord-279463-bli8hwda	242	5	have	have	VBP
cord-279463-bli8hwda	242	6	clearly	clearly	RB
cord-279463-bli8hwda	242	7	shown	show	VBN
cord-279463-bli8hwda	242	8	that	that	IN
cord-279463-bli8hwda	242	9	charged	charge	VBN
cord-279463-bli8hwda	242	10	residues	residue	NNS
cord-279463-bli8hwda	242	11	are	be	VBP
cord-279463-bli8hwda	242	12	not	not	RB
cord-279463-bli8hwda	242	13	essential	essential	JJ
cord-279463-bli8hwda	242	14	for	for	IN
cord-279463-bli8hwda	242	15	membrane	membrane	NN
cord-279463-bli8hwda	242	16	insertion	insertion	NN
cord-279463-bli8hwda	242	17	.	.	.
cord-279463-bli8hwda	243	1	In	in	IN
cord-279463-bli8hwda	243	2	the	the	DT
cord-279463-bli8hwda	243	3	light	light	NN
cord-279463-bli8hwda	243	4	of	of	IN
cord-279463-bli8hwda	243	5	our	-PRON-	PRP$
cord-279463-bli8hwda	243	6	findings	finding	NNS
cord-279463-bli8hwda	243	7	,	,	,
cord-279463-bli8hwda	243	8	we	-PRON-	PRP
cord-279463-bli8hwda	243	9	propose	propose	VBP
cord-279463-bli8hwda	243	10	that	that	IN
cord-279463-bli8hwda	243	11	the	the	DT
cord-279463-bli8hwda	243	12	charged	charge	VBN
cord-279463-bli8hwda	243	13	amino	amino	NN
cord-279463-bli8hwda	243	14	acids	acid	NNS
cord-279463-bli8hwda	243	15	at	at	IN
cord-279463-bli8hwda	243	16	the	the	DT
cord-279463-bli8hwda	243	17	amino	amino	NN
cord-279463-bli8hwda	243	18	terminus	terminus	NN
cord-279463-bli8hwda	243	19	of	of	IN
cord-279463-bli8hwda	243	20	signal	signal	NN
cord-279463-bli8hwda	243	21	sequences	sequence	NNS
cord-279463-bli8hwda	243	22	function	function	VBP
cord-279463-bli8hwda	243	23	in	in	IN
cord-279463-bli8hwda	243	24	the	the	DT
cord-279463-bli8hwda	243	25	alignment	alignment	NN
cord-279463-bli8hwda	243	26	of	of	IN
cord-279463-bli8hwda	243	27	signal	signal	NN
cord-279463-bli8hwda	243	28	sequences	sequence	NNS
cord-279463-bli8hwda	243	29	in	in	IN
cord-279463-bli8hwda	243	30	the	the	DT
cord-279463-bli8hwda	243	31	ER	ER	NNP
cord-279463-bli8hwda	243	32	membrane	membrane	NN
cord-279463-bli8hwda	243	33	such	such	JJ
cord-279463-bli8hwda	243	34	that	that	DT
cord-279463-bli8hwda	243	35	signal	signal	NN
cord-279463-bli8hwda	243	36	peptidase	peptidase	NN
cord-279463-bli8hwda	243	37	can	can	MD
cord-279463-bli8hwda	243	38	cleave	cleave	VB
cord-279463-bli8hwda	243	39	at	at	IN
cord-279463-bli8hwda	243	40	a	a	DT
cord-279463-bli8hwda	243	41	very	very	RB
cord-279463-bli8hwda	243	42	specific	specific	JJ
cord-279463-bli8hwda	243	43	site	site	NN
cord-279463-bli8hwda	243	44	with	with	IN
cord-279463-bli8hwda	243	45	high	high	JJ
cord-279463-bli8hwda	243	46	fidelity	fidelity	NN
cord-279463-bli8hwda	243	47	.	.	.
cord-279463-bli8hwda	244	1	Our	-PRON-	PRP$
cord-279463-bli8hwda	244	2	prediction	prediction	NN
cord-279463-bli8hwda	244	3	is	be	VBZ
cord-279463-bli8hwda	244	4	that	that	IN
cord-279463-bli8hwda	244	5	removal	removal	NN
cord-279463-bli8hwda	244	6	of	of	IN
cord-279463-bli8hwda	244	7	charged	charge	VBN
cord-279463-bli8hwda	244	8	residues	residue	NNS
cord-279463-bli8hwda	244	9	from	from	IN
cord-279463-bli8hwda	244	10	the	the	DT
cord-279463-bli8hwda	244	11	amino	amino	NN
cord-279463-bli8hwda	244	12	-	-	HYPH
cord-279463-bli8hwda	244	13	terminal	terminal	NN
cord-279463-bli8hwda	244	14	end	end	NN
cord-279463-bli8hwda	244	15	of	of	IN
cord-279463-bli8hwda	244	16	the	the	DT
cord-279463-bli8hwda	244	17	signal	signal	NN
cord-279463-bli8hwda	244	18	sequences	sequence	NNS
cord-279463-bli8hwda	244	19	can	can	MD
cord-279463-bli8hwda	244	20	lead	lead	VB
cord-279463-bli8hwda	244	21	to	to	IN
cord-279463-bli8hwda	244	22	an	an	DT
cord-279463-bli8hwda	244	23	altered	altered	JJ
cord-279463-bli8hwda	244	24	or	or	CC
cord-279463-bli8hwda	244	25	less	less	RBR
cord-279463-bli8hwda	244	26	specific	specific	JJ
cord-279463-bli8hwda	244	27	signal	signal	NN
cord-279463-bli8hwda	244	28	peptidase	peptidase	NN
cord-279463-bli8hwda	244	29	cleavage	cleavage	NN
cord-279463-bli8hwda	244	30	.	.	.
cord-279463-bli8hwda	245	1	Wheat	wheat	NN
cord-279463-bli8hwda	245	2	germ	germ	NN
cord-279463-bli8hwda	245	3	was	be	VBD
cord-279463-bli8hwda	245	4	obtained	obtain	VBN
cord-279463-bli8hwda	245	5	from	from	IN
cord-279463-bli8hwda	245	6	General	General	NNP
cord-279463-bli8hwda	245	7	Mills	Mills	NNPS
cord-279463-bli8hwda	245	8	,	,	,
cord-279463-bli8hwda	245	9	California	California	NNP
cord-279463-bli8hwda	245	10	.	.	.
cord-279463-bli8hwda	246	1	The	the	DT
cord-279463-bli8hwda	246	2	acceptor	acceptor	NN
cord-279463-bli8hwda	246	3	peptide	peptide	NN
cord-279463-bli8hwda	246	4	benzoyl	benzoyl	NN
cord-279463-bli8hwda	246	5	-	-	HYPH
cord-279463-bli8hwda	246	6	Asn	Asn	NNP
cord-279463-bli8hwda	246	7	-	-	HYPH
cord-279463-bli8hwda	246	8	LeuThr	LeuThr	NNP
cord-279463-bli8hwda	246	9	-	-	HYPH
cord-279463-bli8hwda	246	10	N	N	NNP
cord-279463-bli8hwda	246	11	-	-	HYPH
cord-279463-bli8hwda	246	12	methylamide	methylamide	NNP
cord-279463-bli8hwda	246	13	was	be	VBD
cord-279463-bli8hwda	246	14	a	a	DT
cord-279463-bli8hwda	246	15	generous	generous	JJ
cord-279463-bli8hwda	246	16	gift	gift	NN
cord-279463-bli8hwda	246	17	from	from	IN
cord-279463-bli8hwda	246	18	E.	E.	NNP
cord-279463-bli8hwda	246	19	Bause	Bause	NNP
cord-279463-bli8hwda	246	20	,	,	,
cord-279463-bli8hwda	246	21	Cologne	Cologne	NNP
cord-279463-bli8hwda	246	22	.	.	.
cord-279463-bli8hwda	247	1	Standard	standard	JJ
cord-279463-bli8hwda	247	2	molecular	molecular	JJ
cord-279463-bli8hwda	247	3	cloning	cloning	NN
cord-279463-bli8hwda	247	4	techniques	technique	NNS
cord-279463-bli8hwda	247	5	,	,	,
cord-279463-bli8hwda	247	6	as	as	IN
cord-279463-bli8hwda	247	7	described	describe	VBN
cord-279463-bli8hwda	247	8	by	by	IN
cord-279463-bli8hwda	247	9	Maniatis	Maniatis	NNP
cord-279463-bli8hwda	247	10	et	et	NNP
cord-279463-bli8hwda	247	11	al	al	NNP
cord-279463-bli8hwda	247	12	.	.	.
cord-279463-bli8hwda	248	1	(	(	-LRB-
cord-279463-bli8hwda	248	2	1962	1962	CD
cord-279463-bli8hwda	248	3	)	)	-RRB-
cord-279463-bli8hwda	248	4	were	be	VBD
cord-279463-bli8hwda	248	5	used	use	VBN
cord-279463-bli8hwda	248	6	.	.	.
cord-279463-bli8hwda	249	1	The	the	DT
cord-279463-bli8hwda	249	2	cDNA	cdna	NN
cord-279463-bli8hwda	249	3	clone	clone	NN
cord-279463-bli8hwda	249	4	py-2	py-2	NNP
cord-279463-bli8hwda	249	5	,	,	,
cord-279463-bli8hwda	249	6	containing	contain	VBG
cord-279463-bli8hwda	249	7	the	the	DT
cord-279463-bli8hwda	249	8	entire	entire	JJ
cord-279463-bli8hwda	249	9	coding	code	VBG
cord-279463-bli8hwda	249	10	region	region	NN
cord-279463-bli8hwda	249	11	of	of	IN
cord-279463-bli8hwda	249	12	the	the	DT
cord-279463-bli8hwda	249	13	human	human	JJ
cord-279463-bli8hwda	249	14	invariant	invariant	JJ
cord-279463-bli8hwda	249	15	chain	chain	NN
cord-279463-bli8hwda	249	16	cloned	clone	VBN
cord-279463-bli8hwda	249	17	into	into	IN
cord-279463-bli8hwda	249	18	the	the	DT
cord-279463-bli8hwda	249	19	Pstl	Pstl	NNP
cord-279463-bli8hwda	249	20	site	site	NN
cord-279463-bli8hwda	249	21	of	of	IN
cord-279463-bli8hwda	249	22	pBR322	pbr322	CD
cord-279463-bli8hwda	249	23	,	,	,
cord-279463-bli8hwda	249	24	was	be	VBD
cord-279463-bli8hwda	249	25	obtained	obtain	VBN
cord-279463-bli8hwda	249	26	from	from	IN
cord-279463-bli8hwda	249	27	P.	P.	NNP
cord-279463-bli8hwda	249	28	A.	a.	NN
cord-279463-bli8hwda	249	29	Peterson	Peterson	NNP
cord-279463-bli8hwda	249	30	's	's	POS
cord-279463-bli8hwda	249	31	laboratory	laboratory	NN
cord-279463-bli8hwda	249	32	,	,	,
cord-279463-bli8hwda	249	33	Uppsala	Uppsala	NNP
cord-279463-bli8hwda	249	34	,	,	,
cord-279463-bli8hwda	249	35	Sweden	Sweden	NNP
cord-279463-bli8hwda	249	36	(	(	-LRB-
cord-279463-bli8hwda	249	37	Claesson	Claesson	NNP
cord-279463-bli8hwda	249	38	et	et	NNP
cord-279463-bli8hwda	249	39	al	al	NNP
cord-279463-bli8hwda	249	40	.	.	NNP
cord-279463-bli8hwda	249	41	,	,	,
cord-279463-bli8hwda	249	42	1963	1963	CD
cord-279463-bli8hwda	249	43	)	)	-RRB-
cord-279463-bli8hwda	249	44	.	.	.
cord-279463-bli8hwda	250	1	The	the	DT
cord-279463-bli8hwda	250	2	expression	expression	NN
cord-279463-bli8hwda	250	3	plasmids	plasmid	VBZ
cord-279463-bli8hwda	250	4	pDS5	pDS5	NNP
cord-279463-bli8hwda	250	5	,	,	,
cord-279463-bli8hwda	250	6	pDS6	pds6	UH
cord-279463-bli8hwda	250	7	,	,	,
cord-279463-bli8hwda	251	1	and	and	CC
cord-279463-bli8hwda	251	2	pDS5/3	pds5/3	DT
cord-279463-bli8hwda	251	3	have	have	VBP
cord-279463-bli8hwda	251	4	been	be	VBN
cord-279463-bli8hwda	251	5	described	describe	VBN
cord-279463-bli8hwda	251	6	previously	previously	RB
cord-279463-bli8hwda	252	1	(	(	-LRB-
cord-279463-bli8hwda	252	2	Stueber	Stueber	NNP
cord-279463-bli8hwda	252	3	et	et	NNP
cord-279463-bli8hwda	252	4	al	al	NNP
cord-279463-bli8hwda	252	5	.	.	NNP
cord-279463-bli8hwda	252	6	,	,	,
cord-279463-bli8hwda	252	7	1964	1964	CD
cord-279463-bli8hwda	252	8	)	)	-RRB-
cord-279463-bli8hwda	252	9	.	.	.
cord-279463-bli8hwda	253	1	They	-PRON-	PRP
cord-279463-bli8hwda	253	2	allow	allow	VBP
cord-279463-bli8hwda	253	3	efficient	efficient	JJ
cord-279463-bli8hwda	253	4	transcription	transcription	NN
cord-279463-bli8hwda	253	5	by	by	IN
cord-279463-bli8hwda	253	6	E.	E.	NNP
cord-279463-bli8hwda	253	7	coli	coli	NNP
cord-279463-bli8hwda	253	8	RNA	RNA	NNP
cord-279463-bli8hwda	253	9	polymerase	polymerase	NN
cord-279463-bli8hwda	253	10	of	of	IN
cord-279463-bli8hwda	253	11	cDNAs	cdna	NNS
cord-279463-bli8hwda	253	12	cloned	clone	VBN
cord-279463-bli8hwda	253	13	behind	behind	IN
cord-279463-bli8hwda	253	14	the	the	DT
cord-279463-bli8hwda	253	15	strong	strong	JJ
cord-279463-bli8hwda	253	16	T5	T5	NNP
cord-279463-bli8hwda	253	17	promoter	promoter	NN
cord-279463-bli8hwda	253	18	P25	P25	NNP
cord-279463-bli8hwda	253	19	.	.	.
cord-279463-bli8hwda	254	1	Figures	figure	NNS
cord-279463-bli8hwda	254	2	9A	9a	VBP
cord-279463-bli8hwda	254	3	and	and	CC
cord-279463-bli8hwda	254	4	9B	9b	NN
cord-279463-bli8hwda	254	5	summarize	summarize	VB
cord-279463-bli8hwda	254	6	the	the	DT
cord-279463-bli8hwda	254	7	construction	construction	NN
cord-279463-bli8hwda	254	8	of	of	IN
cord-279463-bli8hwda	254	9	the	the	DT
cord-279463-bli8hwda	254	10	fusion	fusion	NN
cord-279463-bli8hwda	254	11	and	and	CC
cord-279463-bli8hwda	254	12	deletion	deletion	NN
cord-279463-bli8hwda	254	13	plasmids	plasmid	NNS
cord-279463-bli8hwda	254	14	described	describe	VBN
cord-279463-bli8hwda	254	15	below	below	RB
cord-279463-bli8hwda	254	16	.	.	.
cord-279463-bli8hwda	255	1	plycat	plycat	NNP
cord-279463-bli8hwda	255	2	py-2	py-2	NNP
cord-279463-bli8hwda	255	3	was	be	VBD
cord-279463-bli8hwda	255	4	digested	digest	VBN
cord-279463-bli8hwda	255	5	with	with	IN
cord-279463-bli8hwda	255	6	Pstl	Pstl	NNP
cord-279463-bli8hwda	255	7	,	,	,
cord-279463-bli8hwda	255	8	and	and	CC
cord-279463-bli8hwda	255	9	the	the	DT
cord-279463-bli8hwda	255	10	317	317	CD
cord-279463-bli8hwda	255	11	bp	bp	NNP
cord-279463-bli8hwda	255	12	fragment	fragment	NN
cord-279463-bli8hwda	255	13	containing	contain	VBG
cord-279463-bli8hwda	255	14	the	the	DT
cord-279463-bli8hwda	255	15	5	5	CD
cord-279463-bli8hwda	255	16	'	'	NN
cord-279463-bli8hwda	255	17	end	end	NN
cord-279463-bli8hwda	255	18	and	and	CC
cord-279463-bli8hwda	255	19	the	the	DT
cord-279463-bli8hwda	255	20	660	660	CD
cord-279463-bli8hwda	255	21	bp	bp	NNP
cord-279463-bli8hwda	255	22	fragment	fragment	NN
cord-279463-bli8hwda	255	23	containing	contain	VBG
cord-279463-bli8hwda	255	24	the	the	DT
cord-279463-bli8hwda	255	25	3	3	CD
cord-279463-bli8hwda	255	26	'	'	NN
cord-279463-bli8hwda	255	27	end	end	NN
cord-279463-bli8hwda	255	28	of	of	IN
cord-279463-bli8hwda	255	29	the	the	DT
cord-279463-bli8hwda	255	30	ly	ly	XX
cord-279463-bli8hwda	255	31	coding	code	VBG
cord-279463-bli8hwda	255	32	region	region	NN
cord-279463-bli8hwda	255	33	were	be	VBD
cord-279463-bli8hwda	255	34	isolated	isolate	VBN
cord-279463-bli8hwda	255	35	.	.	.
cord-279463-bli8hwda	256	1	The	the	DT
cord-279463-bli8hwda	256	2	317	317	CD
cord-279463-bli8hwda	256	3	bp	bp	NNP
cord-279463-bli8hwda	256	4	fragment	fragment	NN
cord-279463-bli8hwda	256	5	coding	code	VBG
cord-279463-bli8hwda	256	6	for	for	IN
cord-279463-bli8hwda	256	7	the	the	DT
cord-279463-bli8hwda	256	8	ly	ly	JJ
cord-279463-bli8hwda	256	9	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	256	10	domain	domain	NN
cord-279463-bli8hwda	256	11	,	,	,
cord-279463-bli8hwda	256	12	the	the	DT
cord-279463-bli8hwda	256	13	membrane	membrane	NN
cord-279463-bli8hwda	256	14	-	-	HYPH
cord-279463-bli8hwda	256	15	spanning	span	VBG
cord-279463-bli8hwda	256	16	segment	segment	NN
cord-279463-bli8hwda	256	17	and	and	CC
cord-279463-bli8hwda	256	18	12	12	CD
cord-279463-bli8hwda	256	19	amino	amino	NN
cord-279463-bli8hwda	256	20	acid	acid	NN
cord-279463-bli8hwda	256	21	residues	residue	NNS
cord-279463-bli8hwda	256	22	of	of	IN
cord-279463-bli8hwda	256	23	the	the	DT
cord-279463-bli8hwda	256	24	exoplasmic	exoplasmic	JJ
cord-279463-bli8hwda	256	25	domain	domain	NN
cord-279463-bli8hwda	256	26	,	,	,
cord-279463-bli8hwda	256	27	was	be	VBD
cord-279463-bli8hwda	256	28	cleaved	cleave	VBN
cord-279463-bli8hwda	256	29	by	by	IN
cord-279463-bli8hwda	256	30	SaudA	SaudA	NNP
cord-279463-bli8hwda	256	31	to	to	TO
cord-279463-bli8hwda	256	32	remove	remove	VB
cord-279463-bli8hwda	256	33	the	the	DT
cord-279463-bli8hwda	256	34	SGC	SGC	NNP
cord-279463-bli8hwda	256	35	tail	tail	NN
cord-279463-bli8hwda	256	36	.	.	.
cord-279463-bli8hwda	257	1	The	the	DT
cord-279463-bli8hwda	257	2	234	234	CD
cord-279463-bli8hwda	257	3	bp	bp	NNP
cord-279463-bli8hwda	257	4	Sau3A	Sau3A	NNP
cord-279463-bli8hwda	257	5	-	-	HYPH
cord-279463-bli8hwda	257	6	Pstl	Pstl	NNP
cord-279463-bli8hwda	257	7	fragment	fragment	NN
cord-279463-bli8hwda	257	8	was	be	VBD
cord-279463-bli8hwda	257	9	isolated	isolate	VBN
cord-279463-bli8hwda	257	10	and	and	CC
cord-279463-bli8hwda	257	11	cloned	clone	VBN
cord-279463-bli8hwda	257	12	into	into	IN
cord-279463-bli8hwda	257	13	BamHIIPstl	bamhiipstl	CD
cord-279463-bli8hwda	257	14	-	-	HYPH
cord-279463-bli8hwda	257	15	cut	cut	VBN
cord-279463-bli8hwda	257	16	pDS5	pDS5	NNP
cord-279463-bli8hwda	257	17	.	.	.
cord-279463-bli8hwda	258	1	This	this	DT
cord-279463-bli8hwda	258	2	results	result	VBZ
cord-279463-bli8hwda	258	3	in	in	IN
cord-279463-bli8hwda	258	4	an	an	DT
cord-279463-bli8hwda	258	5	in	in	IN
cord-279463-bli8hwda	258	6	-	-	HYPH
cord-279463-bli8hwda	258	7	frame	frame	NN
cord-279463-bli8hwda	258	8	fusion	fusion	NN
cord-279463-bli8hwda	258	9	of	of	IN
cord-279463-bli8hwda	258	10	the	the	DT
cord-279463-bli8hwda	258	11	5	5	CD
cord-279463-bli8hwda	258	12	'	'	NN
cord-279463-bli8hwda	258	13	end	end	NN
cord-279463-bli8hwda	258	14	of	of	IN
cord-279463-bli8hwda	258	15	ly	ly	XX
cord-279463-bli8hwda	258	16	to	to	IN
cord-279463-bli8hwda	258	17	the	the	DT
cord-279463-bli8hwda	258	18	cat	cat	NN
cord-279463-bli8hwda	258	19	gene	gene	NN
cord-279463-bli8hwda	258	20	.	.	.
cord-279463-bli8hwda	259	1	Ph	Ph	NNP
cord-279463-bli8hwda	259	2	Initial	initial	JJ
cord-279463-bli8hwda	259	3	attempts	attempt	NNS
cord-279463-bli8hwda	259	4	to	to	TO
cord-279463-bli8hwda	259	5	clone	clone	VB
cord-279463-bli8hwda	259	6	the	the	DT
cord-279463-bli8hwda	259	7	completely	completely	RB
cord-279463-bli8hwda	259	8	coding	code	VBG
cord-279463-bli8hwda	259	9	region	region	NN
cord-279463-bli8hwda	259	10	into	into	IN
cord-279463-bli8hwda	259	11	pDS5	pDS5	NNP
cord-279463-bli8hwda	259	12	failed	fail	VBD
cord-279463-bli8hwda	259	13	.	.	.
cord-279463-bli8hwda	260	1	When	when	WRB
cord-279463-bli8hwda	260	2	expressed	express	VBN
cord-279463-bli8hwda	260	3	,	,	,
cord-279463-bli8hwda	260	4	this	this	DT
cord-279463-bli8hwda	260	5	region	region	NN
cord-279463-bli8hwda	260	6	is	be	VBZ
cord-279463-bli8hwda	260	7	probably	probably	RB
cord-279463-bli8hwda	260	8	lethal	lethal	JJ
cord-279463-bli8hwda	260	9	to	to	IN
cord-279463-bli8hwda	260	10	the	the	DT
cord-279463-bli8hwda	260	11	bacterium	bacterium	NN
cord-279463-bli8hwda	260	12	.	.	.
cord-279463-bli8hwda	261	1	To	to	TO
cord-279463-bli8hwda	261	2	repress	repress	VB
cord-279463-bli8hwda	261	3	transcription	transcription	NN
cord-279463-bli8hwda	261	4	from	from	IN
cord-279463-bli8hwda	261	5	the	the	DT
cord-279463-bli8hwda	261	6	T5	T5	NNP
cord-279463-bli8hwda	261	7	promoter	promoter	NN
cord-279463-bli8hwda	261	8	/	/	SYM
cord-279463-bli8hwda	261	9	operator	operator	NN
cord-279463-bli8hwda	261	10	(	(	-LRB-
cord-279463-bli8hwda	261	11	P	p	NN
cord-279463-bli8hwda	261	12	/	/	SYM
cord-279463-bli8hwda	261	13	O	o	NN
cord-279463-bli8hwda	261	14	)	)	-RRB-
cord-279463-bli8hwda	261	15	in	in	IN
cord-279463-bli8hwda	261	16	bacteria	bacteria	NNS
cord-279463-bli8hwda	261	17	,	,	,
cord-279463-bli8hwda	261	18	we	-PRON-	PRP
cord-279463-bli8hwda	261	19	cloned	clone	VBD
cord-279463-bli8hwda	261	20	the	the	DT
cord-279463-bli8hwda	261	21	lac	lac	NN
cord-279463-bli8hwda	261	22	i	i	PRP
cord-279463-bli8hwda	261	23	repressor	repressor	NN
cord-279463-bli8hwda	261	24	between	between	IN
cord-279463-bli8hwda	261	25	the	the	DT
cord-279463-bli8hwda	261	26	b	b	NN
cord-279463-bli8hwda	261	27	/	/	SYM
cord-279463-bli8hwda	261	28	a	a	DT
cord-279463-bli8hwda	261	29	gene	gene	NN
cord-279463-bli8hwda	261	30	and	and	CC
cord-279463-bli8hwda	261	31	the	the	DT
cord-279463-bli8hwda	261	32	T5	T5	NNP
cord-279463-bli8hwda	261	33	P	P	NNP
cord-279463-bli8hwda	261	34	/	/	SYM
cord-279463-bli8hwda	261	35	O.	O.	NNP
cord-279463-bli8hwda	261	36	This	this	DT
cord-279463-bli8hwda	261	37	plasmid	plasmid	NN
cord-279463-bli8hwda	261	38	is	be	VBZ
cord-279463-bli8hwda	261	39	called	call	VBN
cord-279463-bli8hwda	261	40	pfllycat	pfllycat	NN
cord-279463-bli8hwda	261	41	.	.	.
cord-279463-bli8hwda	262	1	For	for	IN
cord-279463-bli8hwda	262	2	the	the	DT
cord-279463-bli8hwda	262	3	construction	construction	NN
cord-279463-bli8hwda	262	4	of	of	IN
cord-279463-bli8hwda	262	5	ply	ply	NN
cord-279463-bli8hwda	262	6	,	,	,
cord-279463-bli8hwda	262	7	pRlycat	pRlycat	NNP
cord-279463-bli8hwda	262	8	was	be	VBD
cord-279463-bli8hwda	262	9	linearized	linearize	VBN
cord-279463-bli8hwda	262	10	by	by	IN
cord-279463-bli8hwda	262	11	Pstl	Pstl	NNP
cord-279463-bli8hwda	262	12	and	and	CC
cord-279463-bli8hwda	262	13	the	the	DT
cord-279463-bli8hwda	262	14	660	660	CD
cord-279463-bli8hwda	262	15	bp	bp	NNP
cord-279463-bli8hwda	262	16	Pstl	Pstl	NNP
cord-279463-bli8hwda	262	17	fragment	fragment	NN
cord-279463-bli8hwda	262	18	,	,	,
cord-279463-bli8hwda	262	19	coding	code	VBG
cord-279463-bli8hwda	262	20	for	for	IN
cord-279463-bli8hwda	262	21	the	the	DT
cord-279463-bli8hwda	262	22	carboxy	carboxy	JJ
cord-279463-bli8hwda	262	23	-	-	HYPH
cord-279463-bli8hwda	262	24	terminal	terminal	NN
cord-279463-bli8hwda	262	25	domain	domain	NN
cord-279463-bli8hwda	262	26	of	of	IN
cord-279463-bli8hwda	262	27	IT	it	NN
cord-279463-bli8hwda	262	28	,	,	,
cord-279463-bli8hwda	262	29	was	be	VBD
cord-279463-bli8hwda	262	30	ligated	ligate	VBN
cord-279463-bli8hwda	262	31	into	into	IN
cord-279463-bli8hwda	262	32	this	this	DT
cord-279463-bli8hwda	262	33	site	site	NN
cord-279463-bli8hwda	262	34	.	.	.
cord-279463-bli8hwda	263	1	Transformants	transformant	NNS
cord-279463-bli8hwda	263	2	containing	contain	VBG
cord-279463-bli8hwda	263	3	the	the	DT
cord-279463-bli8hwda	263	4	660	660	CD
cord-279463-bli8hwda	263	5	bp	bp	NNP
cord-279463-bli8hwda	263	6	fragment	fragment	NN
cord-279463-bli8hwda	263	7	were	be	VBD
cord-279463-bli8hwda	263	8	screened	screen	VBN
cord-279463-bli8hwda	263	9	for	for	IN
cord-279463-bli8hwda	263	10	expression	expression	NN
cord-279463-bli8hwda	263	11	of	of	IN
cord-279463-bli8hwda	263	12	immunoprecipitable	immunoprecipitable	JJ
cord-279463-bli8hwda	263	13	ly	ly	IN
cord-279463-bli8hwda	263	14	chain	chain	NN
cord-279463-bli8hwda	263	15	after	after	IN
cord-279463-bli8hwda	263	16	in	in	IN
cord-279463-bli8hwda	263	17	vitro	vitro	FW
cord-279463-bli8hwda	263	18	transcription	transcription	NN
cord-279463-bli8hwda	263	19	-	-	HYPH
cord-279463-bli8hwda	263	20	translation	translation	NN
cord-279463-bli8hwda	263	21	.	.	.
cord-279463-bli8hwda	264	1	To	to	TO
cord-279463-bli8hwda	264	2	delete	delete	VB
cord-279463-bli8hwda	264	3	the	the	DT
cord-279463-bli8hwda	264	4	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	264	5	domain	domain	NN
cord-279463-bli8hwda	264	6	from	from	IN
cord-279463-bli8hwda	264	7	IrCAT	IrCAT	NNP
cord-279463-bli8hwda	264	8	,	,	,
cord-279463-bli8hwda	264	9	the	the	DT
cord-279463-bli8hwda	264	10	950	950	CD
cord-279463-bli8hwda	264	11	bp	bp	NNP
cord-279463-bli8hwda	264	12	Sstll	Sstll	NNP
cord-279463-bli8hwda	264	13	-	-	HYPH
cord-279463-bli8hwda	264	14	Xbal	Xbal	NNP
cord-279463-bli8hwda	264	15	fragment	fragment	NN
cord-279463-bli8hwda	264	16	from	from	IN
cord-279463-bli8hwda	264	17	plycat	plycat	NNP
cord-279463-bli8hwda	264	18	was	be	VBD
cord-279463-bli8hwda	264	19	isolated	isolate	VBN
cord-279463-bli8hwda	264	20	and	and	CC
cord-279463-bli8hwda	264	21	ligated	ligate	VBN
cord-279463-bli8hwda	264	22	at	at	IN
cord-279463-bli8hwda	264	23	the	the	DT
cord-279463-bli8hwda	264	24	Xbal	Xbal	NNP
cord-279463-bli8hwda	264	25	site	site	NN
cord-279463-bli8hwda	264	26	of	of	IN
cord-279463-bli8hwda	264	27	BamHllXbal	BamHllXbal	NNP
cord-279463-bli8hwda	264	28	cut	cut	VBD
cord-279463-bli8hwda	264	29	p6/5R.	p6/5R.	NNS
cord-279463-bli8hwda	265	1	The	the	DT
cord-279463-bli8hwda	265	2	protruding	protrude	VBG
cord-279463-bli8hwda	265	3	ends	end	VBZ
cord-279463-bli8hwda	265	4	at	at	IN
cord-279463-bli8hwda	265	5	the	the	DT
cord-279463-bli8hwda	265	6	BamHl	BamHl	NNP
cord-279463-bli8hwda	265	7	and	and	CC
cord-279463-bli8hwda	265	8	the	the	DT
cord-279463-bli8hwda	265	9	Sstll	Sstll	NNP
cord-279463-bli8hwda	265	10	sites	site	NNS
cord-279463-bli8hwda	265	11	were	be	VBD
cord-279463-bli8hwda	265	12	blunted	blunt	VBN
cord-279463-bli8hwda	265	13	with	with	IN
cord-279463-bli8hwda	265	14	Sl	sl	NN
cord-279463-bli8hwda	265	15	nuclease	nuclease	NN
cord-279463-bli8hwda	265	16	and	and	CC
cord-279463-bli8hwda	265	17	ligated	ligate	VBN
cord-279463-bli8hwda	265	18	.	.	.
cord-279463-bli8hwda	266	1	As	as	IN
cord-279463-bli8hwda	266	2	a	a	DT
cord-279463-bli8hwda	266	3	result	result	NN
cord-279463-bli8hwda	266	4	,	,	,
cord-279463-bli8hwda	266	5	a	a	DT
cord-279463-bli8hwda	266	6	new	new	JJ
cord-279463-bli8hwda	266	7	ATG	ATG	NNP
cord-279463-bli8hwda	266	8	initiation	initiation	NN
cord-279463-bli8hwda	266	9	codon	codon	NN
cord-279463-bli8hwda	266	10	is	be	VBZ
cord-279463-bli8hwda	266	11	placed	place	VBN
cord-279463-bli8hwda	266	12	just	just	RB
cord-279463-bli8hwda	266	13	in	in	IN
cord-279463-bli8hwda	266	14	front	front	NN
cord-279463-bli8hwda	266	15	of	of	IN
cord-279463-bli8hwda	266	16	the	the	DT
cord-279463-bli8hwda	266	17	membrane	membrane	NN
cord-279463-bli8hwda	266	18	-	-	HYPH
cord-279463-bli8hwda	266	19	spanning	span	VBG
cord-279463-bli8hwda	266	20	segment	segment	NN
cord-279463-bli8hwda	266	21	of	of	IN
cord-279463-bli8hwda	266	22	ly	ly	XX
cord-279463-bli8hwda	266	23	.	.	.
cord-279463-bli8hwda	267	1	The	the	DT
cord-279463-bli8hwda	267	2	construction	construction	NN
cord-279463-bli8hwda	267	3	was	be	VBD
cord-279463-bli8hwda	267	4	confirmed	confirm	VBN
cord-279463-bli8hwda	267	5	by	by	IN
cord-279463-bli8hwda	267	6	DNA	dna	NN
cord-279463-bli8hwda	267	7	and	and	CC
cord-279463-bli8hwda	267	8	amino	amino	NN
cord-279463-bli8hwda	267	9	acid	acid	NN
cord-279463-bli8hwda	267	10	sequence	sequence	NN
cord-279463-bli8hwda	267	11	analyses	analysis	NNS
cord-279463-bli8hwda	267	12	(	(	-LRB-
cord-279463-bli8hwda	267	13	see	see	VB
cord-279463-bli8hwda	267	14	Figure	figure	NN
cord-279463-bli8hwda	267	15	4A	4a	CD
cord-279463-bli8hwda	267	16	)	)	-RRB-
cord-279463-bli8hwda	267	17	.	.	.
cord-279463-bli8hwda	268	1	p6lSR	p6lSR	NNP
cord-279463-bli8hwda	269	1	To	to	TO
cord-279463-bli8hwda	269	2	repress	repress	VB
cord-279463-bli8hwda	269	3	transcription	transcription	NN
cord-279463-bli8hwda	269	4	from	from	IN
cord-279463-bli8hwda	269	5	the	the	DT
cord-279463-bli8hwda	269	6	T5	T5	NNP
cord-279463-bli8hwda	269	7	promoter	promoter	NN
cord-279463-bli8hwda	269	8	the	the	DT
cord-279463-bli8hwda	269	9	lac	lac	NN
cord-279463-bli8hwda	270	1	i	i	PRP
cord-279463-bli8hwda	270	2	gene	gene	NN
cord-279463-bli8hwda	270	3	was	be	VBD
cord-279463-bli8hwda	270	4	inserted	insert	VBN
cord-279463-bli8hwda	270	5	between	between	IN
cord-279463-bli8hwda	270	6	the	the	DT
cord-279463-bli8hwda	270	7	b	b	NN
cord-279463-bli8hwda	270	8	/	/	SYM
cord-279463-bli8hwda	270	9	a	a	DT
cord-279463-bli8hwda	270	10	gene	gene	NN
cord-279463-bli8hwda	270	11	and	and	CC
cord-279463-bli8hwda	270	12	the	the	DT
cord-279463-bli8hwda	270	13	T5	T5	NNP
cord-279463-bli8hwda	270	14	P	P	NNP
cord-279463-bli8hwda	270	15	/	/	SYM
cord-279463-bli8hwda	270	16	O	o	NN
cord-279463-bli8hwda	270	17	region	region	NN
cord-279463-bli8hwda	270	18	of	of	IN
cord-279463-bli8hwda	270	19	pDS5/3	pds5/3	XX
cord-279463-bli8hwda	271	1	(	(	-LRB-
cord-279463-bli8hwda	271	2	Stueber	Stueber	NNP
cord-279463-bli8hwda	271	3	et	et	NNP
cord-279463-bli8hwda	271	4	al	al	NNP
cord-279463-bli8hwda	271	5	.	.	NNP
cord-279463-bli8hwda	271	6	,	,	,
cord-279463-bli8hwda	271	7	1984	1984	CD
cord-279463-bli8hwda	271	8	)	)	-RRB-
cord-279463-bli8hwda	271	9	.	.	.
cord-279463-bli8hwda	272	1	Against	against	IN
cord-279463-bli8hwda	272	2	ly	ly	XX
cord-279463-bli8hwda	272	3	Domains	domain	NNS
cord-279463-bli8hwda	272	4	To	to	TO
cord-279463-bli8hwda	272	5	raise	raise	VB
cord-279463-bli8hwda	272	6	antibodies	antibody	NNS
cord-279463-bli8hwda	272	7	against	against	IN
cord-279463-bli8hwda	272	8	the	the	DT
cord-279463-bli8hwda	272	9	amino	amino	NN
cord-279463-bli8hwda	272	10	-	-	:
cord-279463-bli8hwda	272	11	and	and	CC
cord-279463-bli8hwda	272	12	the	the	DT
cord-279463-bli8hwda	272	13	carboxy	carboxy	JJ
cord-279463-bli8hwda	272	14	-	-	HYPH
cord-279463-bli8hwda	272	15	terminal	terminal	NN
cord-279463-bli8hwda	272	16	domains	domain	NNS
cord-279463-bli8hwda	272	17	of	of	IN
cord-279463-bli8hwda	272	18	ly	ly	NN
cord-279463-bli8hwda	272	19	,	,	,
cord-279463-bli8hwda	272	20	fusion	fusion	NN
cord-279463-bli8hwda	272	21	proteins	protein	NNS
cord-279463-bli8hwda	272	22	of	of	IN
cord-279463-bli8hwda	272	23	B	b	NN
cord-279463-bli8hwda	272	24	-	-	HYPH
cord-279463-bli8hwda	272	25	galactosidase	galactosidase	NN
cord-279463-bli8hwda	272	26	and	and	CC
cord-279463-bli8hwda	272	27	parts	part	NNS
cord-279463-bli8hwda	272	28	of	of	IN
cord-279463-bli8hwda	272	29	ly	ly	NNS
cord-279463-bli8hwda	272	30	were	be	VBD
cord-279463-bli8hwda	272	31	produced	produce	VBN
cord-279463-bli8hwda	272	32	in	in	IN
cord-279463-bli8hwda	272	33	bacteria	bacteria	NNS
cord-279463-bli8hwda	272	34	and	and	CC
cord-279463-bli8hwda	272	35	used	use	VBN
cord-279463-bli8hwda	272	36	as	as	IN
cord-279463-bli8hwda	272	37	antigens	antigen	NNS
cord-279463-bli8hwda	272	38	to	to	TO
cord-279463-bli8hwda	272	39	raise	raise	VB
cord-279463-bli8hwda	272	40	antibodies	antibody	NNS
cord-279463-bli8hwda	272	41	in	in	IN
cord-279463-bli8hwda	272	42	rabbits	rabbit	NNS
cord-279463-bli8hwda	272	43	.	.	.
cord-279463-bli8hwda	273	1	From	from	IN
cord-279463-bli8hwda	273	2	a	a	DT
cord-279463-bli8hwda	273	3	Pstl	Pstl	NNP
cord-279463-bli8hwda	273	4	digest	digest	NN
cord-279463-bli8hwda	273	5	of	of	IN
cord-279463-bli8hwda	273	6	w-2	w-2	NNP
cord-279463-bli8hwda	273	7	,	,	,
cord-279463-bli8hwda	273	8	the	the	DT
cord-279463-bli8hwda	273	9	317	317	CD
cord-279463-bli8hwda	273	10	bp	bp	NNP
cord-279463-bli8hwda	273	11	fragment	fragment	NN
cord-279463-bli8hwda	273	12	coding	code	VBG
cord-279463-bli8hwda	273	13	for	for	IN
cord-279463-bli8hwda	273	14	the	the	DT
cord-279463-bli8hwda	273	15	aminoterminal	aminoterminal	JJ
cord-279463-bli8hwda	273	16	72	72	CD
cord-279463-bli8hwda	273	17	amino	amino	JJ
cord-279463-bli8hwda	273	18	acids	acid	NNS
cord-279463-bli8hwda	273	19	of	of	IN
cord-279463-bli8hwda	273	20	ly	ly	NNS
cord-279463-bli8hwda	273	21	and	and	CC
cord-279463-bli8hwda	273	22	the	the	DT
cord-279463-bli8hwda	273	23	860	860	CD
cord-279463-bli8hwda	273	24	bp	bp	NNP
cord-279463-bli8hwda	273	25	fragment	fragment	NN
cord-279463-bli8hwda	273	26	coding	code	VBG
cord-279463-bli8hwda	273	27	for	for	IN
cord-279463-bli8hwda	273	28	the	the	DT
cord-279463-bli8hwda	273	29	exoplasmic	exoplasmic	NNP
cord-279463-bli8hwda	273	30	carboxy	carboxy	JJ
cord-279463-bli8hwda	273	31	-	-	HYPH
cord-279463-bli8hwda	273	32	terminal	terminal	NN
cord-279463-bli8hwda	273	33	domain	domain	NN
cord-279463-bli8hwda	273	34	of	of	IN
cord-279463-bli8hwda	273	35	ly	ly	NNS
cord-279463-bli8hwda	273	36	were	be	VBD
cord-279463-bli8hwda	273	37	isolated	isolate	VBN
cord-279463-bli8hwda	273	38	.	.	.
cord-279463-bli8hwda	274	1	Each	each	DT
cord-279463-bli8hwda	274	2	of	of	IN
cord-279463-bli8hwda	274	3	the	the	DT
cord-279463-bli8hwda	274	4	fragments	fragment	NNS
cord-279463-bli8hwda	274	5	was	be	VBD
cord-279463-bli8hwda	274	6	inserted	insert	VBN
cord-279463-bli8hwda	274	7	into	into	IN
cord-279463-bli8hwda	274	8	the	the	DT
cord-279463-bli8hwda	274	9	Pstl	Pstl	NNP
cord-279463-bli8hwda	274	10	site	site	NN
cord-279463-bli8hwda	274	11	of	of	IN
cord-279463-bli8hwda	274	12	the	the	DT
cord-279463-bli8hwda	274	13	bacterial	bacterial	JJ
cord-279463-bli8hwda	274	14	expression	expression	NN
cord-279463-bli8hwda	274	15	vector	vector	NN
cord-279463-bli8hwda	274	16	pEX1	pEX1	NNP
cord-279463-bli8hwda	275	1	(	(	-LRB-
cord-279463-bli8hwda	275	2	Stanley	Stanley	NNP
cord-279463-bli8hwda	275	3	and	and	CC
cord-279463-bli8hwda	275	4	Luzio	Luzio	NNP
cord-279463-bli8hwda	275	5	,	,	,
cord-279463-bli8hwda	275	6	1984	1984	CD
cord-279463-bli8hwda	275	7	)	)	-RRB-
cord-279463-bli8hwda	275	8	.	.	.
cord-279463-bli8hwda	276	1	Fusion	fusion	NN
cord-279463-bli8hwda	276	2	proteins	protein	NNS
cord-279463-bli8hwda	276	3	expressed	express	VBN
cord-279463-bli8hwda	276	4	in	in	IN
cord-279463-bli8hwda	276	5	NFl	nfl	JJ
cord-279463-bli8hwda	276	6	bacteria	bacteria	NNS
cord-279463-bli8hwda	276	7	were	be	VBD
cord-279463-bli8hwda	276	8	separated	separate	VBN
cord-279463-bli8hwda	276	9	on	on	IN
cord-279463-bli8hwda	276	10	preparative	preparative	JJ
cord-279463-bli8hwda	276	11	SDS	sds	NN
cord-279463-bli8hwda	276	12	-	-	HYPH
cord-279463-bli8hwda	276	13	polyacrylamide	polyacrylamide	NN
cord-279463-bli8hwda	276	14	gels	gel	NNS
cord-279463-bli8hwda	276	15	(	(	-LRB-
cord-279463-bli8hwda	276	16	7	7	CD
cord-279463-bli8hwda	276	17	%	%	NN
cord-279463-bli8hwda	276	18	acrylamide	acrylamide	NN
cord-279463-bli8hwda	276	19	;	;	:
cord-279463-bli8hwda	276	20	Laemmli	Laemmli	NNP
cord-279463-bli8hwda	276	21	,	,	,
cord-279463-bli8hwda	276	22	1970	1970	CD
cord-279463-bli8hwda	276	23	)	)	-RRB-
cord-279463-bli8hwda	276	24	.	.	.
cord-279463-bli8hwda	277	1	Protein	protein	NN
cord-279463-bli8hwda	277	2	bands	band	NNS
cord-279463-bli8hwda	277	3	were	be	VBD
cord-279463-bli8hwda	277	4	visualized	visualize	VBN
cord-279463-bli8hwda	277	5	by	by	IN
cord-279463-bli8hwda	277	6	KOAc	KOAc	NNS
cord-279463-bli8hwda	277	7	precipitation	precipitation	NN
cord-279463-bli8hwda	277	8	,	,	,
cord-279463-bli8hwda	277	9	and	and	CC
cord-279463-bli8hwda	277	10	fusion	fusion	NN
cord-279463-bli8hwda	277	11	proteins	protein	NNS
cord-279463-bli8hwda	277	12	were	be	VBD
cord-279463-bli8hwda	277	13	eluted	elute	VBN
cord-279463-bli8hwda	277	14	from	from	IN
cord-279463-bli8hwda	277	15	gel	gel	NN
cord-279463-bli8hwda	277	16	slices	slice	NNS
cord-279463-bli8hwda	277	17	.	.	.
cord-279463-bli8hwda	278	1	Two	two	CD
cord-279463-bli8hwda	278	2	rabbits	rabbit	NNS
cord-279463-bli8hwda	278	3	were	be	VBD
cord-279463-bli8hwda	278	4	immunized	immunize	VBN
cord-279463-bli8hwda	278	5	with	with	IN
cord-279463-bli8hwda	278	6	each	each	DT
cord-279463-bli8hwda	278	7	of	of	IN
cord-279463-bli8hwda	278	8	the	the	DT
cord-279463-bli8hwda	278	9	two	two	CD
cord-279463-bli8hwda	278	10	fusion	fusion	NN
cord-279463-bli8hwda	278	11	proteins	protein	NNS
cord-279463-bli8hwda	278	12	.	.	.
cord-279463-bli8hwda	279	1	Antibodies	antibody	NNS
cord-279463-bli8hwda	279	2	against	against	IN
cord-279463-bli8hwda	279	3	the	the	DT
cord-279463-bli8hwda	279	4	amino	amino	NN
cord-279463-bli8hwda	279	5	terminus	terminus	NN
cord-279463-bli8hwda	279	6	of	of	IN
cord-279463-bli8hwda	279	7	ly	ly	JJ
cord-279463-bli8hwda	279	8	(	(	-LRB-
cord-279463-bli8hwda	279	9	anti	anti	JJ
cord-279463-bli8hwda	279	10	-	-	JJ
cord-279463-bli8hwda	279	11	IyN	iyn	JJ
cord-279463-bli8hwda	279	12	)	)	-RRB-
cord-279463-bli8hwda	279	13	and	and	CC
cord-279463-bli8hwda	279	14	its	-PRON-	PRP$
cord-279463-bli8hwda	279	15	carboxyl	carboxyl	NN
cord-279463-bli8hwda	279	16	terminus	terminus	NN
cord-279463-bli8hwda	279	17	(	(	-LRB-
cord-279463-bli8hwda	279	18	anti	anti	JJ
cord-279463-bli8hwda	279	19	-	-	JJ
cord-279463-bli8hwda	279	20	IyC	iyc	JJ
cord-279463-bli8hwda	279	21	)	)	-RRB-
cord-279463-bli8hwda	279	22	were	be	VBD
cord-279463-bli8hwda	279	23	obtained	obtain	VBN
cord-279463-bli8hwda	279	24	.	.	.
cord-279463-bli8hwda	280	1	They	-PRON-	PRP
cord-279463-bli8hwda	280	2	reacted	react	VBD
cord-279463-bli8hwda	280	3	with	with	IN
cord-279463-bli8hwda	280	4	authentic	authentic	JJ
cord-279463-bli8hwda	280	5	ly	ly	IN
cord-279463-bli8hwda	280	6	chains	chain	NNS
cord-279463-bli8hwda	280	7	synthesized	synthesize	VBN
cord-279463-bli8hwda	280	8	by	by	IN
cord-279463-bli8hwda	280	9	human	human	JJ
cord-279463-bli8hwda	280	10	Raji	Raji	NNP
cord-279463-bli8hwda	280	11	cells	cell	NNS
cord-279463-bli8hwda	280	12	(	(	-LRB-
cord-279463-bli8hwda	280	13	data	datum	NNS
cord-279463-bli8hwda	280	14	not	not	RB
cord-279463-bli8hwda	280	15	shown	show	VBN
cord-279463-bli8hwda	280	16	)	)	-RRB-
cord-279463-bli8hwda	280	17	.	.	.
cord-279463-bli8hwda	281	1	lmmunoprecipitations	lmmunoprecipitation	NNS
cord-279463-bli8hwda	281	2	After	after	IN
cord-279463-bli8hwda	281	3	translation	translation	NN
cord-279463-bli8hwda	281	4	and	and	CC
cord-279463-bli8hwda	281	5	posttranslational	posttranslational	JJ
cord-279463-bli8hwda	281	6	assays	assay	NNS
cord-279463-bli8hwda	281	7	,	,	,
cord-279463-bli8hwda	282	1	antigens	antigen	NNS
cord-279463-bli8hwda	282	2	in	in	IN
cord-279463-bli8hwda	282	3	a	a	DT
cord-279463-bli8hwda	282	4	25	25	CD
cord-279463-bli8hwda	282	5	VI	VI	NNP
cord-279463-bli8hwda	282	6	aliquot	aliquot	NN
cord-279463-bli8hwda	282	7	were	be	VBD
cord-279463-bli8hwda	282	8	solubilized	solubilize	VBN
cord-279463-bli8hwda	282	9	by	by	IN
cord-279463-bli8hwda	282	10	adding	add	VBG
cord-279463-bli8hwda	282	11	Nonidet	Nonidet	NNP
cord-279463-bli8hwda	282	12	-	-	HYPH
cord-279463-bli8hwda	282	13	P40	P40	NNP
cord-279463-bli8hwda	282	14	(	(	-LRB-
cord-279463-bli8hwda	282	15	NP40	NP40	NNP
cord-279463-bli8hwda	282	16	)	)	-RRB-
cord-279463-bli8hwda	282	17	to	to	IN
cord-279463-bli8hwda	282	18	0.5	0.5	CD
cord-279463-bli8hwda	282	19	%	%	NN
cord-279463-bli8hwda	282	20	.	.	.
cord-279463-bli8hwda	283	1	Then	then	RB
cord-279463-bli8hwda	283	2	1	1	CD
cord-279463-bli8hwda	283	3	~1	~1	CD
cord-279463-bli8hwda	283	4	of	of	IN
cord-279463-bli8hwda	283	5	either	either	DT
cord-279463-bli8hwda	283	6	anti	anti	JJ
cord-279463-bli8hwda	283	7	-	-	JJ
cord-279463-bli8hwda	283	8	IyN	iyn	JJ
cord-279463-bli8hwda	283	9	or	or	CC
cord-279463-bli8hwda	283	10	anti+C	anti+c	IN
cord-279463-bli8hwda	283	11	antiserum	antiserum	NN
cord-279463-bli8hwda	283	12	was	be	VBD
cord-279463-bli8hwda	283	13	added	add	VBN
cord-279463-bli8hwda	283	14	and	and	CC
cord-279463-bli8hwda	283	15	the	the	DT
cord-279463-bli8hwda	283	16	mixtures	mixture	NNS
cord-279463-bli8hwda	283	17	incubated	incubate	VBN
cord-279463-bli8hwda	283	18	for	for	IN
cord-279463-bli8hwda	283	19	15	15	CD
cord-279463-bli8hwda	283	20	min	min	NN
cord-279463-bli8hwda	283	21	at	at	IN
cord-279463-bli8hwda	283	22	4OC	4oc	CD
cord-279463-bli8hwda	283	23	.	.	.
cord-279463-bli8hwda	284	1	Forty	forty	CD
cord-279463-bli8hwda	284	2	microliters	microliter	NNS
cord-279463-bli8hwda	284	3	of	of	IN
cord-279463-bli8hwda	284	4	a	a	DT
cord-279463-bli8hwda	284	5	1	1	CD
cord-279463-bli8hwda	284	6	:	:	:
cord-279463-bli8hwda	284	7	l	l	NN
cord-279463-bli8hwda	284	8	slurry	slurry	NN
cord-279463-bli8hwda	284	9	of	of	IN
cord-279463-bli8hwda	284	10	protein	protein	NN
cord-279463-bli8hwda	284	11	A	A	NNP
cord-279463-bli8hwda	284	12	-	-	:
cord-279463-bli8hwda	284	13	Sepharose	Sepharose	NNP
cord-279463-bli8hwda	284	14	(	(	-LRB-
cord-279463-bli8hwda	284	15	equilibrated	equilibrate	VBN
cord-279463-bli8hwda	284	16	in	in	IN
cord-279463-bli8hwda	284	17	0.2	0.2	CD
cord-279463-bli8hwda	284	18	%	%	NN
cord-279463-bli8hwda	284	19	NP40,lO	NP40,lO	NNP
cord-279463-bli8hwda	284	20	mM	mM	NNP
cord-279463-bli8hwda	284	21	Tris	Tris	NNP
cord-279463-bli8hwda	284	22	-	-	HYPH
cord-279463-bli8hwda	284	23	HCI	HCI	NNP
cord-279463-bli8hwda	285	1	[	[	-LRB-
cord-279463-bli8hwda	285	2	pH	pH	NNP
cord-279463-bli8hwda	285	3	7.51	7.51	CD
cord-279463-bli8hwda	285	4	,	,	,
cord-279463-bli8hwda	285	5	150	150	CD
cord-279463-bli8hwda	285	6	mM	mm	CD
cord-279463-bli8hwda	285	7	NaCI	naci	NN
cord-279463-bli8hwda	285	8	,	,	,
cord-279463-bli8hwda	285	9	and	and	CC
cord-279463-bli8hwda	285	10	2	2	CD
cord-279463-bli8hwda	285	11	mM	mm	CD
cord-279463-bli8hwda	285	12	EDTA	EDTA	NNP
cord-279463-bli8hwda	285	13	)	)	-RRB-
cord-279463-bli8hwda	285	14	was	be	VBD
cord-279463-bli8hwda	285	15	added	add	VBN
cord-279463-bli8hwda	285	16	to	to	IN
cord-279463-bli8hwda	285	17	each	each	DT
cord-279463-bli8hwda	285	18	sample	sample	NN
cord-279463-bli8hwda	285	19	,	,	,
cord-279463-bli8hwda	285	20	and	and	CC
cord-279463-bli8hwda	285	21	incubation	incubation	NN
cord-279463-bli8hwda	285	22	continued	continue	VBD
cord-279463-bli8hwda	285	23	for	for	IN
cord-279463-bli8hwda	285	24	60	60	CD
cord-279463-bli8hwda	285	25	min	min	NN
cord-279463-bli8hwda	285	26	at	at	IN
cord-279463-bli8hwda	285	27	4	4	CD
cord-279463-bli8hwda	285	28	°	°	NN
cord-279463-bli8hwda	285	29	C	c	NN
cord-279463-bli8hwda	285	30	.	.	.
cord-279463-bli8hwda	286	1	Beads	bead	NNS
cord-279463-bli8hwda	286	2	were	be	VBD
cord-279463-bli8hwda	286	3	sedimented	sediment	VBN
cord-279463-bli8hwda	286	4	by	by	IN
cord-279463-bli8hwda	286	5	centrifugation	centrifugation	NN
cord-279463-bli8hwda	286	6	and	and	CC
cord-279463-bli8hwda	286	7	washed	wash	VBD
cord-279463-bli8hwda	286	8	three	three	CD
cord-279463-bli8hwda	286	9	times	time	NNS
cord-279463-bli8hwda	286	10	with	with	IN
cord-279463-bli8hwda	286	11	0.2	0.2	CD
cord-279463-bli8hwda	286	12	%	%	NN
cord-279463-bli8hwda	287	1	NP40	NP40	NNP
cord-279463-bli8hwda	287	2	,	,	,
cord-279463-bli8hwda	287	3	10	10	CD
cord-279463-bli8hwda	287	4	mM	mM	NNP
cord-279463-bli8hwda	287	5	Tris	Tris	NNP
cord-279463-bli8hwda	287	6	-	-	HYPH
cord-279463-bli8hwda	287	7	HCI	HCI	NNP
cord-279463-bli8hwda	287	8	(	(	-LRB-
cord-279463-bli8hwda	287	9	pH	pH	NNP
cord-279463-bli8hwda	287	10	7.5	7.5	CD
cord-279463-bli8hwda	287	11	)	)	-RRB-
cord-279463-bli8hwda	287	12	,	,	,
cord-279463-bli8hwda	287	13	150	150	CD
cord-279463-bli8hwda	287	14	mM	mm	CD
cord-279463-bli8hwda	287	15	NaCI	naci	NN
cord-279463-bli8hwda	287	16	,	,	,
cord-279463-bli8hwda	287	17	and	and	CC
cord-279463-bli8hwda	287	18	2	2	CD
cord-279463-bli8hwda	287	19	mM	mm	CD
cord-279463-bli8hwda	287	20	EDTA	EDTA	NNP
cord-279463-bli8hwda	287	21	,	,	,
cord-279463-bli8hwda	287	22	twice	twice	RB
cord-279463-bli8hwda	287	23	with	with	IN
cord-279463-bli8hwda	287	24	0.2	0.2	CD
cord-279463-bli8hwda	287	25	%	%	NN
cord-279463-bli8hwda	287	26	NP40,lO	NP40,lO	NNP
cord-279463-bli8hwda	287	27	mM	mM	NNP
cord-279463-bli8hwda	287	28	Tris	Tris	NNP
cord-279463-bli8hwda	287	29	-	-	HYPH
cord-279463-bli8hwda	287	30	HCI	HCI	NNP
cord-279463-bli8hwda	287	31	(	(	-LRB-
cord-279463-bli8hwda	287	32	pH	pH	NNP
cord-279463-bli8hwda	287	33	7.5	7.5	CD
cord-279463-bli8hwda	287	34	)	)	-RRB-
cord-279463-bli8hwda	287	35	,	,	,
cord-279463-bli8hwda	287	36	500	500	CD
cord-279463-bli8hwda	287	37	mM	mm	CD
cord-279463-bli8hwda	287	38	NaCI	naci	NN
cord-279463-bli8hwda	287	39	,	,	,
cord-279463-bli8hwda	287	40	and	and	CC
cord-279463-bli8hwda	287	41	2	2	CD
cord-279463-bli8hwda	287	42	mM	mm	CD
cord-279463-bli8hwda	287	43	EDTA	EDTA	NNP
cord-279463-bli8hwda	287	44	,	,	,
cord-279463-bli8hwda	287	45	and	and	CC
cord-279463-bli8hwda	287	46	once	once	RB
cord-279463-bli8hwda	287	47	with	with	IN
cord-279463-bli8hwda	287	48	10	10	CD
cord-279463-bli8hwda	287	49	mM	mM	NNP
cord-279463-bli8hwda	287	50	Tris	Tris	NNP
cord-279463-bli8hwda	287	51	-	-	HYPH
cord-279463-bli8hwda	287	52	HCI	HCI	NNP
cord-279463-bli8hwda	287	53	(	(	-LRB-
cord-279463-bli8hwda	287	54	pH	pH	NNP
cord-279463-bli8hwda	287	55	7.5	7.5	CD
cord-279463-bli8hwda	287	56	)	)	-RRB-
cord-279463-bli8hwda	287	57	.	.	.
cord-279463-bli8hwda	288	1	Sample	sample	JJ
cord-279463-bli8hwda	288	2	buffer	buffer	NN
cord-279463-bli8hwda	288	3	for	for	IN
cord-279463-bli8hwda	288	4	SDS	SDS	NNP
cord-279463-bli8hwda	288	5	-	-	HYPH
cord-279463-bli8hwda	288	6	PAGE	PAGE	NNP
cord-279463-bli8hwda	288	7	was	be	VBD
cord-279463-bli8hwda	288	8	added	add	VBN
cord-279463-bli8hwda	288	9	to	to	IN
cord-279463-bli8hwda	288	10	the	the	DT
cord-279463-bli8hwda	288	11	sedimented	sedimented	JJ
cord-279463-bli8hwda	288	12	beads	bead	NNS
cord-279463-bli8hwda	288	13	,	,	,
cord-279463-bli8hwda	288	14	and	and	CC
cord-279463-bli8hwda	288	15	antigens	antigen	NNS
cord-279463-bli8hwda	288	16	were	be	VBD
cord-279463-bli8hwda	288	17	analyzed	analyze	VBN
cord-279463-bli8hwda	288	18	by	by	IN
cord-279463-bli8hwda	288	19	SDS	SDS	NNP
cord-279463-bli8hwda	288	20	-	-	HYPH
cord-279463-bli8hwda	288	21	PAGE	PAGE	NNP
cord-279463-bli8hwda	288	22	and	and	CC
cord-279463-bli8hwda	288	23	fluorography	fluorography	NN
cord-279463-bli8hwda	288	24	.	.	.
cord-279463-bli8hwda	289	1	In	in	IN
cord-279463-bli8hwda	289	2	Vitro	Vitro	NNP
cord-279463-bli8hwda	289	3	Transcription	Transcription	NNP
cord-279463-bli8hwda	289	4	and	and	CC
cord-279463-bli8hwda	289	5	Translation	Translation	NNP
cord-279463-bli8hwda	289	6	Plasmids	Plasmids	NNPS
cord-279463-bli8hwda	289	7	were	be	VBD
cord-279463-bli8hwda	289	8	transcribed	transcribe	VBN
cord-279463-bli8hwda	289	9	in	in	IN
cord-279463-bli8hwda	289	10	vitro	vitro	FW
cord-279463-bli8hwda	289	11	by	by	IN
cord-279463-bli8hwda	289	12	E.	E.	NNP
cord-279463-bli8hwda	289	13	coli	coli	NNP
cord-279463-bli8hwda	289	14	RNA	RNA	NNP
cord-279463-bli8hwda	289	15	polymerase	polymerase	NN
cord-279463-bli8hwda	289	16	,	,	,
cord-279463-bli8hwda	289	17	and	and	CC
cord-279463-bli8hwda	289	18	the	the	DT
cord-279463-bli8hwda	289	19	resulting	result	VBG
cord-279463-bli8hwda	289	20	mRNA	mrna	NN
cord-279463-bli8hwda	289	21	was	be	VBD
cord-279463-bli8hwda	289	22	translated	translate	VBN
cord-279463-bli8hwda	289	23	in	in	IN
cord-279463-bli8hwda	289	24	a	a	DT
cord-279463-bli8hwda	289	25	wheat	wheat	NN
cord-279463-bli8hwda	289	26	germ	germ	NN
cord-279463-bli8hwda	289	27	cell	cell	NN
cord-279463-bli8hwda	289	28	-	-	HYPH
cord-279463-bli8hwda	289	29	free	free	JJ
cord-279463-bli8hwda	289	30	system	system	NN
cord-279463-bli8hwda	289	31	as	as	IN
cord-279463-bli8hwda	289	32	described	describe	VBN
cord-279463-bli8hwda	289	33	by	by	IN
cord-279463-bli8hwda	289	34	Stueber	Stueber	NNP
cord-279463-bli8hwda	289	35	et	et	NNP
cord-279463-bli8hwda	289	36	al	al	NNP
cord-279463-bli8hwda	289	37	.	.	.
cord-279463-bli8hwda	290	1	(	(	-LRB-
cord-279463-bli8hwda	290	2	1984	1984	CD
cord-279463-bli8hwda	290	3	)	)	-RRB-
cord-279463-bli8hwda	290	4	.	.	.
cord-279463-bli8hwda	291	1	To	to	TO
cord-279463-bli8hwda	291	2	test	test	VB
cord-279463-bli8hwda	291	3	for	for	IN
cord-279463-bli8hwda	291	4	membrane	membrane	NN
cord-279463-bli8hwda	291	5	translocation	translocation	NN
cord-279463-bli8hwda	291	6	,	,	,
cord-279463-bli8hwda	291	7	rough	rough	JJ
cord-279463-bli8hwda	291	8	microsomes	microsome	NNS
cord-279463-bli8hwda	291	9	from	from	IN
cord-279463-bli8hwda	291	10	dog	dog	NN
cord-279463-bli8hwda	291	11	pancreas	pancrea	NNS
cord-279463-bli8hwda	291	12	were	be	VBD
cord-279463-bli8hwda	291	13	included	include	VBN
cord-279463-bli8hwda	291	14	in	in	IN
cord-279463-bli8hwda	291	15	the	the	DT
cord-279463-bli8hwda	291	16	translation	translation	NN
cord-279463-bli8hwda	291	17	(	(	-LRB-
cord-279463-bli8hwda	291	18	Blobel	Blobel	NNP
cord-279463-bli8hwda	291	19	and	and	CC
cord-279463-bli8hwda	291	20	Dobberstein	Dobberstein	NNP
cord-279463-bli8hwda	291	21	,	,	,
cord-279463-bli8hwda	291	22	1975	1975	CD
cord-279463-bli8hwda	291	23	)	)	-RRB-
cord-279463-bli8hwda	291	24	.	.	.
cord-279463-bli8hwda	292	1	Glycosylation	glycosylation	NN
cord-279463-bli8hwda	292	2	onto	onto	IN
cord-279463-bli8hwda	292	3	asparagine	asparagine	NN
cord-279463-bli8hwda	292	4	residues	residue	NNS
cord-279463-bli8hwda	292	5	was	be	VBD
cord-279463-bli8hwda	292	6	blocked	block	VBN
cord-279463-bli8hwda	292	7	by	by	IN
cord-279463-bli8hwda	292	8	the	the	DT
cord-279463-bli8hwda	292	9	addition	addition	NN
cord-279463-bli8hwda	292	10	of	of	IN
cord-279463-bli8hwda	292	11	the	the	DT
cord-279463-bli8hwda	292	12	acceptor	acceptor	NN
cord-279463-bli8hwda	292	13	peptide	peptide	NN
cord-279463-bli8hwda	292	14	benzoylAsn	benzoylasn	NN
cord-279463-bli8hwda	292	15	-	-	HYPH
cord-279463-bli8hwda	292	16	LeuThr	LeuThr	NNP
cord-279463-bli8hwda	292	17	-	-	HYPH
cord-279463-bli8hwda	292	18	N	N	NNP
cord-279463-bli8hwda	292	19	-	-	HYPH
cord-279463-bli8hwda	292	20	methylamide	methylamide	NN
cord-279463-bli8hwda	292	21	to	to	IN
cord-279463-bli8hwda	292	22	a	a	DT
cord-279463-bli8hwda	292	23	final	final	JJ
cord-279463-bli8hwda	292	24	concentration	concentration	NN
cord-279463-bli8hwda	292	25	of	of	IN
cord-279463-bli8hwda	292	26	30	30	CD
cord-279463-bli8hwda	292	27	pM	pm	NN
cord-279463-bli8hwda	293	1	(	(	-LRB-
cord-279463-bli8hwda	293	2	Lau	Lau	NNP
cord-279463-bli8hwda	293	3	et	et	NNP
cord-279463-bli8hwda	293	4	al	al	NNP
cord-279463-bli8hwda	293	5	.	.	NNP
cord-279463-bli8hwda	293	6	,	,	,
cord-279463-bli8hwda	293	7	1983	1983	CD
cord-279463-bli8hwda	294	1	;	;	:
cord-279463-bli8hwda	294	2	Bause	Bause	NNP
cord-279463-bli8hwda	294	3	,	,	,
cord-279463-bli8hwda	294	4	1983	1983	CD
cord-279463-bli8hwda	294	5	)	)	-RRB-
cord-279463-bli8hwda	294	6	.	.	.
cord-279463-bli8hwda	295	1	Assays	assay	NNS
cord-279463-bli8hwda	296	1	To	to	TO
cord-279463-bli8hwda	296	2	test	test	VB
cord-279463-bli8hwda	296	3	translocation	translocation	NN
cord-279463-bli8hwda	296	4	of	of	IN
cord-279463-bli8hwda	296	5	in	in	IN
cord-279463-bli8hwda	296	6	vitro	vitro	FW
cord-279463-bli8hwda	296	7	-	-	HYPH
cord-279463-bli8hwda	296	8	synthesized	synthesize	VBN
cord-279463-bli8hwda	296	9	proteins	protein	NNS
cord-279463-bli8hwda	296	10	across	across	IN
cord-279463-bli8hwda	296	11	,	,	,
cord-279463-bli8hwda	296	12	or	or	CC
cord-279463-bli8hwda	296	13	their	-PRON-	PRP$
cord-279463-bli8hwda	296	14	insertion	insertion	NN
cord-279463-bli8hwda	296	15	into	into	IN
cord-279463-bli8hwda	296	16	,	,	,
cord-279463-bli8hwda	296	17	the	the	DT
cord-279463-bli8hwda	296	18	ER	ER	NNP
cord-279463-bli8hwda	296	19	membrane	membrane	NN
cord-279463-bli8hwda	296	20	,	,	,
cord-279463-bli8hwda	296	21	accessibility	accessibility	NN
cord-279463-bli8hwda	296	22	to	to	IN
cord-279463-bli8hwda	296	23	proteinase	proteinase	NN
cord-279463-bli8hwda	296	24	K	K	NNP
cord-279463-bli8hwda	296	25	was	be	VBD
cord-279463-bli8hwda	296	26	used	use	VBN
cord-279463-bli8hwda	296	27	.	.	.
cord-279463-bli8hwda	297	1	A	a	DT
cord-279463-bli8hwda	297	2	10	10	CD
cord-279463-bli8hwda	297	3	pl	pl	NN
cord-279463-bli8hwda	297	4	aliquot	aliquot	NN
cord-279463-bli8hwda	297	5	of	of	IN
cord-279463-bli8hwda	297	6	a	a	DT
cord-279463-bli8hwda	297	7	translation	translation	NN
cord-279463-bli8hwda	297	8	mixture	mixture	NN
cord-279463-bli8hwda	297	9	containing	contain	VBG
cord-279463-bli8hwda	297	10	rough	rough	JJ
cord-279463-bli8hwda	297	11	microsomes	microsome	NNS
cord-279463-bli8hwda	297	12	was	be	VBD
cord-279463-bli8hwda	297	13	incubated	incubate	VBN
cord-279463-bli8hwda	297	14	for	for	IN
cord-279463-bli8hwda	297	15	10	10	CD
cord-279463-bli8hwda	297	16	min	min	NN
cord-279463-bli8hwda	297	17	at	at	IN
cord-279463-bli8hwda	297	18	25OC	25oc	CD
cord-279463-bli8hwda	297	19	with	with	IN
cord-279463-bli8hwda	297	20	either	either	CC
cord-279463-bli8hwda	297	21	0.3	0.3	CD
cord-279463-bli8hwda	297	22	mg	mg	NNP
cord-279463-bli8hwda	297	23	/	/	SYM
cord-279463-bli8hwda	297	24	ml	ml	NNS
cord-279463-bli8hwda	297	25	of	of	IN
cord-279463-bli8hwda	297	26	proteinase	proteinase	NN
cord-279463-bli8hwda	297	27	K	K	NNP
cord-279463-bli8hwda	297	28	or	or	CC
cord-279463-bli8hwda	297	29	0.3	0.3	CD
cord-279463-bli8hwda	297	30	mg	mg	NNP
cord-279463-bli8hwda	297	31	/	/	SYM
cord-279463-bli8hwda	297	32	ml	ml	NNS
cord-279463-bli8hwda	297	33	of	of	IN
cord-279463-bli8hwda	297	34	proteinase	proteinase	NN
cord-279463-bli8hwda	297	35	K	k	NN
cord-279463-bli8hwda	297	36	and	and	CC
cord-279463-bli8hwda	297	37	0.5	0.5	CD
cord-279463-bli8hwda	297	38	%	%	NN
cord-279463-bli8hwda	298	1	NP40	NP40	NNP
cord-279463-bli8hwda	298	2	.	.	.
cord-279463-bli8hwda	299	1	Further	further	JJ
cord-279463-bli8hwda	299	2	proteolysis	proteolysis	NN
cord-279463-bli8hwda	299	3	was	be	VBD
cord-279463-bli8hwda	299	4	stopped	stop	VBN
cord-279463-bli8hwda	299	5	by	by	IN
cord-279463-bli8hwda	299	6	the	the	DT
cord-279463-bli8hwda	299	7	addition	addition	NN
cord-279463-bli8hwda	299	8	of	of	IN
cord-279463-bli8hwda	299	9	phenylmethylsulfonyl	phenylmethylsulfonyl	NN
cord-279463-bli8hwda	299	10	fluoride	fluoride	NN
cord-279463-bli8hwda	299	11	(	(	-LRB-
cord-279463-bli8hwda	299	12	PMSF	PMSF	NNP
cord-279463-bli8hwda	299	13	)	)	-RRB-
cord-279463-bli8hwda	299	14	to	to	IN
cord-279463-bli8hwda	299	15	0.1	0.1	CD
cord-279463-bli8hwda	299	16	mglml	mglml	NNS
cord-279463-bli8hwda	299	17	,	,	,
cord-279463-bli8hwda	299	18	and	and	CC
cord-279463-bli8hwda	299	19	the	the	DT
cord-279463-bli8hwda	299	20	sample	sample	NN
cord-279463-bli8hwda	299	21	was	be	VBD
cord-279463-bli8hwda	299	22	further	further	RB
cord-279463-bli8hwda	299	23	characterized	characterize	VBN
cord-279463-bli8hwda	299	24	by	by	IN
cord-279463-bli8hwda	299	25	SDS	SDS	NNP
cord-279463-bli8hwda	299	26	-	-	HYPH
cord-279463-bli8hwda	299	27	PAGE	PAGE	NNP
cord-279463-bli8hwda	299	28	(	(	-LRB-
cord-279463-bli8hwda	299	29	Laemmli	Laemmli	NNP
cord-279463-bli8hwda	299	30	,	,	,
cord-279463-bli8hwda	299	31	1970	1970	CD
cord-279463-bli8hwda	299	32	)	)	-RRB-
cord-279463-bli8hwda	299	33	and	and	CC
cord-279463-bli8hwda	299	34	fluorography	fluorography	NN
cord-279463-bli8hwda	299	35	or	or	CC
cord-279463-bli8hwda	299	36	,	,	,
cord-279463-bli8hwda	299	37	where	where	WRB
cord-279463-bli8hwda	299	38	indicated	indicate	VBN
cord-279463-bli8hwda	299	39	in	in	IN
cord-279463-bli8hwda	299	40	the	the	DT
cord-279463-bli8hwda	299	41	figure	figure	NN
cord-279463-bli8hwda	299	42	,	,	,
cord-279463-bli8hwda	299	43	by	by	IN
cord-279463-bli8hwda	299	44	immunoprecipitation	immunoprecipitation	NN
cord-279463-bli8hwda	299	45	.	.	.
cord-279463-bli8hwda	300	1	To	to	TO
cord-279463-bli8hwda	300	2	remove	remove	VB
cord-279463-bli8hwda	300	3	secretory	secretory	JJ
cord-279463-bli8hwda	300	4	and	and	CC
cord-279463-bli8hwda	300	5	peripheral	peripheral	JJ
cord-279463-bli8hwda	300	6	membrane	membrane	NN
cord-279463-bli8hwda	300	7	proteins	protein	NNS
cord-279463-bli8hwda	300	8	,	,	,
cord-279463-bli8hwda	300	9	rough	rough	JJ
cord-279463-bli8hwda	300	10	microsomes	microsome	NNS
cord-279463-bli8hwda	300	11	were	be	VBD
cord-279463-bli8hwda	300	12	subjected	subject	VBN
cord-279463-bli8hwda	300	13	to	to	IN
cord-279463-bli8hwda	300	14	a	a	DT
cord-279463-bli8hwda	300	15	carbonate	carbonate	NN
cord-279463-bli8hwda	300	16	wash	wash	NN
cord-279463-bli8hwda	300	17	with	with	IN
cord-279463-bli8hwda	300	18	0.1	0.1	CD
cord-279463-bli8hwda	300	19	M	M	NNP
cord-279463-bli8hwda	300	20	Na&Os	Na&Os	NNP
cord-279463-bli8hwda	300	21	,	,	,
cord-279463-bli8hwda	300	22	pH	pH	NNP
cord-279463-bli8hwda	300	23	11	11	CD
cord-279463-bli8hwda	301	1	(	(	-LRB-
cord-279463-bli8hwda	301	2	Fujiki	Fujiki	NNP
cord-279463-bli8hwda	301	3	et	et	NNP
cord-279463-bli8hwda	301	4	al	al	NNP
cord-279463-bli8hwda	301	5	.	.	NNP
cord-279463-bli8hwda	301	6	,	,	,
cord-279463-bli8hwda	301	7	1982	1982	CD
cord-279463-bli8hwda	301	8	)	)	-RRB-
cord-279463-bli8hwda	301	9	.	.	.
cord-279463-bli8hwda	302	1	peptide	peptide	NN
cord-279463-bli8hwda	302	2	;	;	:
cord-279463-bli8hwda	302	3	H.	H.	NNP
cord-279463-bli8hwda	302	4	Gausepohl	Gausepohl	NNP
cord-279463-bli8hwda	302	5	for	for	IN
cord-279463-bli8hwda	302	6	performing	perform	VBG
cord-279463-bli8hwda	302	7	automated	automate	VBN
cord-279463-bli8hwda	302	8	amino	amino	NN
cord-279463-bli8hwda	302	9	acid	acid	NN
cord-279463-bli8hwda	302	10	analysis	analysis	NN
cord-279463-bli8hwda	302	11	:	:	:
cord-279463-bli8hwda	303	1	M.	M.	NNP
cord-279463-bli8hwda	303	2	T.	T.	NNP
cord-279463-bli8hwda	303	3	Haeuptle	Haeuptle	NNP
cord-279463-bli8hwda	303	4	,	,	,
cord-279463-bli8hwda	303	5	I.	I.	NNP
cord-279463-bli8hwda	303	6	Ibrahimi	Ibrahimi	NNP
cord-279463-bli8hwda	303	7	,	,	,
cord-279463-bli8hwda	303	8	and	and	CC
cord-279463-bli8hwda	303	9	D.	D.	NNP
cord-279463-bli8hwda	303	10	Meyer	Meyer	NNP
cord-279463-bli8hwda	303	11	for	for	IN
cord-279463-bli8hwda	303	12	critical	critical	JJ
cord-279463-bli8hwda	303	13	reading	reading	NN
cord-279463-bli8hwda	303	14	of	of	IN
cord-279463-bli8hwda	303	15	the	the	DT
cord-279463-bli8hwda	303	16	manuscript	manuscript	NN
cord-279463-bli8hwda	303	17	,	,	,
cord-279463-bli8hwda	303	18	and	and	CC
cord-279463-bli8hwda	303	19	Annie	Annie	NNP
cord-279463-bli8hwda	303	20	Steiner	Steiner	NNP
cord-279463-bli8hwda	303	21	for	for	IN
cord-279463-bli8hwda	303	22	expert	expert	JJ
cord-279463-bli8hwda	303	23	typing	typing	NN
cord-279463-bli8hwda	303	24	.	.	.
cord-279463-bli8hwda	304	1	This	this	DT
cord-279463-bli8hwda	304	2	work	work	NN
cord-279463-bli8hwda	304	3	was	be	VBD
cord-279463-bli8hwda	304	4	supported	support	VBN
cord-279463-bli8hwda	304	5	by	by	IN
cord-279463-bli8hwda	304	6	grant	grant	NN
cord-279463-bli8hwda	304	7	DO	DO	NNP
cord-279463-bli8hwda	304	8	199/4-Z	199/4-z	CD
cord-279463-bli8hwda	304	9	from	from	IN
cord-279463-bli8hwda	304	10	the	the	DT
cord-279463-bli8hwda	304	11	Deutsche	Deutsche	NNP
cord-279463-bli8hwda	304	12	Forschungsgemeinschaft	Forschungsgemeinschaft	NNP
cord-279463-bli8hwda	304	13	.	.	.
cord-279463-bli8hwda	305	1	The	the	DT
cord-279463-bli8hwda	305	2	costs	cost	NNS
cord-279463-bli8hwda	305	3	of	of	IN
cord-279463-bli8hwda	305	4	publication	publication	NN
cord-279463-bli8hwda	305	5	of	of	IN
cord-279463-bli8hwda	305	6	this	this	DT
cord-279463-bli8hwda	305	7	article	article	NN
cord-279463-bli8hwda	305	8	were	be	VBD
cord-279463-bli8hwda	305	9	defrayed	defray	VBN
cord-279463-bli8hwda	305	10	in	in	IN
cord-279463-bli8hwda	305	11	part	part	NN
cord-279463-bli8hwda	305	12	by	by	IN
cord-279463-bli8hwda	305	13	the	the	DT
cord-279463-bli8hwda	305	14	payment	payment	NN
cord-279463-bli8hwda	305	15	of	of	IN
cord-279463-bli8hwda	305	16	page	page	NN
cord-279463-bli8hwda	305	17	charges	charge	NNS
cord-279463-bli8hwda	305	18	.	.	.
cord-279463-bli8hwda	306	1	This	this	DT
cord-279463-bli8hwda	306	2	article	article	NN
cord-279463-bli8hwda	306	3	must	must	MD
cord-279463-bli8hwda	306	4	therefore	therefore	RB
cord-279463-bli8hwda	306	5	be	be	VB
cord-279463-bli8hwda	306	6	hereby	hereby	RB
cord-279463-bli8hwda	306	7	marked	mark	VBN
cord-279463-bli8hwda	306	8	"	"	``
cord-279463-bli8hwda	306	9	advertisement	advertisement	NN
cord-279463-bli8hwda	306	10	"	"	''
cord-279463-bli8hwda	306	11	in	in	IN
cord-279463-bli8hwda	306	12	accordance	accordance	NN
cord-279463-bli8hwda	306	13	with	with	IN
cord-279463-bli8hwda	306	14	18	18	CD
cord-279463-bli8hwda	306	15	USC	USC	NNP
cord-279463-bli8hwda	306	16	.	.	.
cord-279463-bli8hwda	307	1	Section	section	NN
cord-279463-bli8hwda	307	2	1734	1734	CD
cord-279463-bli8hwda	307	3	solely	solely	RB
cord-279463-bli8hwda	307	4	to	to	TO
cord-279463-bli8hwda	307	5	indicate	indicate	VB
cord-279463-bli8hwda	307	6	this	this	DT
cord-279463-bli8hwda	307	7	fact	fact	NN
cord-279463-bli8hwda	307	8	.	.	.
cord-279463-bli8hwda	308	1	Received	receive	VBN
cord-279463-bli8hwda	308	2	April	April	NNP
cord-279463-bli8hwda	308	3	23	23	CD
cord-279463-bli8hwda	308	4	,	,	,
cord-279463-bli8hwda	308	5	1986	1986	CD
cord-279463-bli8hwda	308	6	;	;	,
cord-279463-bli8hwda	308	7	revised	revise	VBN
cord-279463-bli8hwda	308	8	July	July	NNP
cord-279463-bli8hwda	308	9	1	1	CD
cord-279463-bli8hwda	308	10	,	,	,
cord-279463-bli8hwda	308	11	1986	1986	CD
cord-279463-bli8hwda	308	12	.	.	.
cord-279463-bli8hwda	309	1	Multiple	multiple	JJ
cord-279463-bli8hwda	309	2	mechanisms	mechanism	NNS
cord-279463-bli8hwda	309	3	of	of	IN
cord-279463-bli8hwda	309	4	protein	protein	NN
cord-279463-bli8hwda	309	5	insertion	insertion	NN
cord-279463-bli8hwda	309	6	into	into	IN
cord-279463-bli8hwda	309	7	and	and	CC
cord-279463-bli8hwda	309	8	across	across	IN
cord-279463-bli8hwda	309	9	membranes	membrane	NNS
cord-279463-bli8hwda	310	1	A	a	DT
cord-279463-bli8hwda	310	2	stop	stop	NN
cord-279463-bli8hwda	310	3	transfer	transfer	NN
cord-279463-bli8hwda	310	4	sequence	sequence	NN
cord-279463-bli8hwda	310	5	confers	confer	VBZ
cord-279463-bli8hwda	310	6	predictable	predictable	JJ
cord-279463-bli8hwda	310	7	transmembrane	transmembrane	NN
cord-279463-bli8hwda	310	8	orientation	orientation	NN
cord-279463-bli8hwda	310	9	to	to	IN
cord-279463-bli8hwda	310	10	a	a	DT
cord-279463-bli8hwda	310	11	previously	previously	RB
cord-279463-bli8hwda	310	12	secreted	secrete	VBN
cord-279463-bli8hwda	310	13	protein	protein	NN
cord-279463-bli8hwda	310	14	in	in	IN
cord-279463-bli8hwda	310	15	cell	cell	NN
cord-279463-bli8hwda	310	16	-	-	HYPH
cord-279463-bli8hwda	310	17	free	free	JJ
cord-279463-bli8hwda	310	18	systems	system	NNS
cord-279463-bli8hwda	310	19	Clonal	Clonal	NNP
cord-279463-bli8hwda	310	20	variation	variation	NN
cord-279463-bli8hwda	310	21	in	in	IN
cord-279463-bli8hwda	310	22	cell	cell	NN
cord-279463-bli8hwda	310	23	surface	surface	NN
cord-279463-bli8hwda	310	24	display	display	NN
cord-279463-bli8hwda	310	25	of	of	IN
cord-279463-bli8hwda	310	26	an	an	DT
cord-279463-bli8hwda	310	27	H-2	H-2	NNP
cord-279463-bli8hwda	310	28	protein	protein	NN
cord-279463-bli8hwda	310	29	lacking	lack	VBG
cord-279463-bli8hwda	310	30	a	a	DT
cord-279463-bli8hwda	310	31	cytoplasmic	cytoplasmic	JJ
cord-279463-bli8hwda	310	32	tail	tail	NN
cord-279463-bli8hwda	311	1	We	-PRON-	PRP
cord-279463-bli8hwda	311	2	thank	thank	VBP
cord-279463-bli8hwda	311	3	P	p	NN
cord-279463-bli8hwda	311	4	A.	a.	NN
cord-279463-bli8hwda	311	5	Peterson	Peterson	NNP
cord-279463-bli8hwda	311	6	and	and	CC
cord-279463-bli8hwda	311	7	L.	L.	NNP
cord-279463-bli8hwda	311	8	Claesson	Claesson	NNP
cord-279463-bli8hwda	311	9	,	,	,
cord-279463-bli8hwda	311	10	Uppsala	Uppsala	NNP
cord-279463-bli8hwda	311	11	,	,	,
cord-279463-bli8hwda	311	12	for	for	IN
cord-279463-bli8hwda	311	13	plasmid	plasmid	NN
cord-279463-bli8hwda	311	14	py-2	py-2	NNP
cord-279463-bli8hwda	311	15	;	;	:
cord-279463-bli8hwda	311	16	E.	E.	NNP
cord-279463-bli8hwda	311	17	Bause	Bause	NNP
cord-279463-bli8hwda	311	18	,	,	,
cord-279463-bli8hwda	311	19	Cologne	Cologne	NNP
cord-279463-bli8hwda	311	20	,	,	,
cord-279463-bli8hwda	311	21	for	for	IN
cord-279463-bli8hwda	311	22	the	the	DT
cord-279463-bli8hwda	311	23	acceptor	acceptor	NN
cord-279463-bli8hwda	311	24	Abrahamsen	Abrahamsen	NNP
cord-279463-bli8hwda	311	25	,	,	,
cord-279463-bli8hwda	311	26	L.	L.	NNP
cord-279463-bli8hwda	311	27	,	,	,
cord-279463-bli8hwda	311	28	Moks	Moks	NNP
cord-279463-bli8hwda	311	29	,	,	,
cord-279463-bli8hwda	311	30	T.	T.	NNP
cord-279463-bli8hwda	311	31	,	,	,
cord-279463-bli8hwda	311	32	Nilsson	Nilsson	NNP
cord-279463-bli8hwda	311	33	,	,	,
cord-279463-bli8hwda	311	34	B.	B.	NNP
cord-279463-bli8hwda	311	35	,	,	,
cord-279463-bli8hwda	311	36	Hellman	Hellman	NNP
cord-279463-bli8hwda	311	37	,	,	,
cord-279463-bli8hwda	311	38	U.	U.	NNP
cord-279463-bli8hwda	311	39	,	,	,
cord-279463-bli8hwda	311	40	and	and	CC
cord-279463-bli8hwda	311	41	Uhlen	Uhlen	NNP
cord-279463-bli8hwda	311	42	,	,	,
cord-279463-bli8hwda	311	43	M.	M.	NNP
cord-279463-bli8hwda	311	44	(	(	-LRB-
cord-279463-bli8hwda	311	45	1985	1985	CD
cord-279463-bli8hwda	311	46	)	)	-RRB-
cord-279463-bli8hwda	311	47	.	.	.
cord-279463-bli8hwda	312	1	Analysis	analysis	NN
cord-279463-bli8hwda	312	2	of	of	IN
cord-279463-bli8hwda	312	3	signals	signal	NNS
cord-279463-bli8hwda	312	4	for	for	IN
cord-279463-bli8hwda	312	5	secretion	secretion	NN
cord-279463-bli8hwda	312	6	in	in	IN
cord-279463-bli8hwda	312	7	the	the	DT
cord-279463-bli8hwda	312	8	staphylococcal	staphylococcal	JJ
cord-279463-bli8hwda	312	9	protein	protein	NN
cord-279463-bli8hwda	312	10	A	a	DT
cord-279463-bli8hwda	312	11	gene	gene	NN
cord-279463-bli8hwda	312	12	.	.	.
cord-279463-bli8hwda	313	1	EMBO	EMBO	NNP
cord-279463-bli8hwda	313	2	J.	J.	NNP
cord-279463-bli8hwda	313	3	4	4	CD
cord-279463-bli8hwda	313	4	,	,	,
cord-279463-bli8hwda	313	5	3901	3901	CD
cord-279463-bli8hwda	313	6	-	-	SYM
cord-279463-bli8hwda	313	7	3906	3906	CD
cord-279463-bli8hwda	313	8	.	.	.
cord-279463-bli8hwda	314	1	Adams	Adams	NNP
cord-279463-bli8hwda	314	2	,	,	,
cord-279463-bli8hwda	314	3	G.	G.	NNP
cord-279463-bli8hwda	314	4	A.	A.	NNP
cord-279463-bli8hwda	314	5	,	,	,
cord-279463-bli8hwda	314	6	and	and	CC
cord-279463-bli8hwda	314	7	Rose	Rose	NNP
cord-279463-bli8hwda	314	8	,	,	,
cord-279463-bli8hwda	314	9	J.	J.	NNP
cord-279463-bli8hwda	314	10	K.	K.	NNP
cord-279463-bli8hwda	314	11	(	(	-LRB-
cord-279463-bli8hwda	314	12	1985	1985	CD
cord-279463-bli8hwda	314	13	)	)	-RRB-
cord-279463-bli8hwda	314	14	.	.	.
cord-279463-bli8hwda	315	1	Structural	structural	JJ
cord-279463-bli8hwda	315	2	requirements	requirement	NNS
cord-279463-bli8hwda	315	3	for	for	IN
cord-279463-bli8hwda	315	4	a	a	DT
cord-279463-bli8hwda	315	5	membrane	membrane	NN
cord-279463-bli8hwda	315	6	-	-	HYPH
cord-279463-bli8hwda	315	7	spanning	span	VBG
cord-279463-bli8hwda	315	8	domain	domain	NN
cord-279463-bli8hwda	315	9	for	for	IN
cord-279463-bli8hwda	315	10	protein	protein	NN
cord-279463-bli8hwda	315	11	anchoring	anchoring	NN
cord-279463-bli8hwda	315	12	and	and	CC
cord-279463-bli8hwda	315	13	cell	cell	NN
cord-279463-bli8hwda	315	14	surface	surface	NN
cord-279463-bli8hwda	315	15	transport	transport	NN
cord-279463-bli8hwda	315	16	.	.	.
cord-279463-bli8hwda	316	1	Cell	cell	NN
cord-279463-bli8hwda	316	2	47	47	CD
cord-279463-bli8hwda	316	3	,	,	,
cord-279463-bli8hwda	316	4	lOOi-1015.Anderson	lOOi-1015.Anderson	NNP
cord-279463-bli8hwda	316	5	,	,	,
cord-279463-bli8hwda	316	6	D.	D.	NNP
cord-279463-bli8hwda	316	7	J.	J.	NNP
cord-279463-bli8hwda	316	8	,	,	,
cord-279463-bli8hwda	316	9	Mostov	Mostov	NNP
cord-279463-bli8hwda	316	10	,	,	,
cord-279463-bli8hwda	316	11	K.	K.	NNP
cord-279463-bli8hwda	316	12	E.	E.	NNP
cord-279463-bli8hwda	316	13	,	,	,
cord-279463-bli8hwda	316	14	and	and	CC
cord-279463-bli8hwda	316	15	Blob	Blob	NNP
cord-279463-bli8hwda	316	16	&	&	CC
cord-279463-bli8hwda	316	17	G.	G.	NNP
cord-279463-bli8hwda	316	18	(	(	-LRB-
cord-279463-bli8hwda	316	19	1983	1983	CD
cord-279463-bli8hwda	316	20	)	)	-RRB-
cord-279463-bli8hwda	316	21	.	.	.
cord-279463-bli8hwda	317	1	Mechanisms	mechanism	NNS
cord-279463-bli8hwda	317	2	of	of	IN
cord-279463-bli8hwda	317	3	integration	integration	NN
cord-279463-bli8hwda	317	4	of	of	IN
cord-279463-bli8hwda	317	5	de	de	NNP
cord-279463-bli8hwda	317	6	novo	novo	NNP
cord-279463-bli8hwda	317	7	-	-	HYPH
cord-279463-bli8hwda	317	8	synthesized	synthesize	VBN
cord-279463-bli8hwda	317	9	polypeptides	polypeptide	NNS
cord-279463-bli8hwda	317	10	into	into	IN
cord-279463-bli8hwda	317	11	membranes	membrane	NNS
cord-279463-bli8hwda	317	12	:	:	:
cord-279463-bli8hwda	317	13	signal	signal	NN
cord-279463-bli8hwda	317	14	recognition	recognition	NN
cord-279463-bli8hwda	317	15	particle	particle	NN
cord-279463-bli8hwda	317	16	is	be	VBZ
cord-279463-bli8hwda	317	17	required	require	VBN
cord-279463-bli8hwda	317	18	for	for	IN
cord-279463-bli8hwda	317	19	integration	integration	NN
cord-279463-bli8hwda	317	20	into	into	IN
cord-279463-bli8hwda	317	21	microsomal	microsomal	JJ
cord-279463-bli8hwda	317	22	membranes	membrane	NNS
cord-279463-bli8hwda	317	23	of	of	IN
cord-279463-bli8hwda	317	24	calcium	calcium	NN
cord-279463-bli8hwda	317	25	ATPase	atpase	NN
cord-279463-bli8hwda	317	26	and	and	CC
cord-279463-bli8hwda	317	27	of	of	IN
cord-279463-bli8hwda	317	28	lens	lens	NN
cord-279463-bli8hwda	318	1	MP26	mp26	NN
cord-279463-bli8hwda	318	2	but	but	CC
cord-279463-bli8hwda	318	3	not	not	RB
cord-279463-bli8hwda	318	4	of	of	IN
cord-279463-bli8hwda	318	5	cytochrome	cytochrome	NN
cord-279463-bli8hwda	318	6	be	be	VB
cord-279463-bli8hwda	318	7	.	.	.
cord-279463-bli8hwda	319	1	Proc	proc	VB
cord-279463-bli8hwda	319	2	.	.	.
cord-279463-bli8hwda	320	1	Natl	Natl	NNP
cord-279463-bli8hwda	320	2	.	.	.
cord-279463-bli8hwda	321	1	Acad	Acad	NNP
cord-279463-bli8hwda	321	2	.	.	.
cord-279463-bli8hwda	322	1	Sci	Sci	NNP
cord-279463-bli8hwda	322	2	.	.	NNP
cord-279463-bli8hwda	322	3	USA	USA	NNP
cord-279463-bli8hwda	322	4	80	80	CD
cord-279463-bli8hwda	322	5	,	,	,
cord-279463-bli8hwda	322	6	7249	7249	CD
cord-279463-bli8hwda	322	7	-	-	HYPH
cord-279463-bli8hwda	322	8	7253	7253	CD
cord-279463-bli8hwda	322	9	.	.	.
cord-279463-bli8hwda	323	1	Bause	Bause	NNP
cord-279463-bli8hwda	323	2	,	,	,
cord-279463-bli8hwda	323	3	E.	E.	NNP
cord-279463-bli8hwda	323	4	(	(	-LRB-
cord-279463-bli8hwda	323	5	1983	1983	CD
cord-279463-bli8hwda	323	6	)	)	-RRB-
cord-279463-bli8hwda	323	7	.	.	.
cord-279463-bli8hwda	324	1	Structural	structural	JJ
cord-279463-bli8hwda	324	2	requirements	requirement	NNS
cord-279463-bli8hwda	324	3	of	of	IN
cord-279463-bli8hwda	324	4	N	n	CD
cord-279463-bli8hwda	324	5	-	-	HYPH
cord-279463-bli8hwda	324	6	glycosylation	glycosylation	NN
cord-279463-bli8hwda	324	7	of	of	IN
cord-279463-bli8hwda	324	8	proteins	protein	NNS
cord-279463-bli8hwda	324	9	.	.	.
cord-279463-bli8hwda	325	1	Biochem	Biochem	NNP
cord-279463-bli8hwda	325	2	.	.	.
cord-279463-bli8hwda	326	1	J.	J.	NNP
cord-279463-bli8hwda	326	2	209	209	CD
cord-279463-bli8hwda	326	3	,	,	,
cord-279463-bli8hwda	326	4	331	331	CD
cord-279463-bli8hwda	326	5	-	-	SYM
cord-279463-bli8hwda	326	6	336	336	CD
cord-279463-bli8hwda	326	7	.	.	.
cord-279463-bli8hwda	327	1	Blobel	Blobel	NNP
cord-279463-bli8hwda	327	2	,	,	,
cord-279463-bli8hwda	327	3	G.	G.	NNP
cord-279463-bli8hwda	327	4	,	,	,
cord-279463-bli8hwda	327	5	and	and	CC
cord-279463-bli8hwda	327	6	Dobberstein	Dobberstein	NNP
cord-279463-bli8hwda	327	7	,	,	,
cord-279463-bli8hwda	327	8	B.	B.	NNP
cord-279463-bli8hwda	327	9	(	(	-LRB-
cord-279463-bli8hwda	327	10	1975	1975	CD
cord-279463-bli8hwda	327	11	)	)	-RRB-
cord-279463-bli8hwda	328	1	Lingappa	Lingappa	NNP
cord-279463-bli8hwda	328	2	,	,	,
cord-279463-bli8hwda	328	3	V.	V.	NNP
cord-279463-bli8hwda	328	4	R.	R.	NNP
cord-279463-bli8hwda	328	5	,	,	,
cord-279463-bli8hwda	328	6	Katz	Katz	NNP
cord-279463-bli8hwda	328	7	,	,	,
cord-279463-bli8hwda	328	8	F.	F.	NNP
cord-279463-bli8hwda	328	9	N.	N.	NNP
cord-279463-bli8hwda	328	10	,	,	,
cord-279463-bli8hwda	328	11	Lodish	Lodish	NNP
cord-279463-bli8hwda	328	12	,	,	,
cord-279463-bli8hwda	328	13	H.	H.	NNP
cord-279463-bli8hwda	328	14	F.	F.	NNP
cord-279463-bli8hwda	328	15	,	,	,
cord-279463-bli8hwda	328	16	and	and	CC
cord-279463-bli8hwda	328	17	Blobel	Blobel	NNP
cord-279463-bli8hwda	328	18	,	,	,
cord-279463-bli8hwda	328	19	G.	G.	NNP
cord-279463-bli8hwda	328	20	(	(	-LRB-
cord-279463-bli8hwda	328	21	1978	1978	CD
cord-279463-bli8hwda	328	22	)	)	-RRB-
cord-279463-bli8hwda	328	23	.	.	.
cord-279463-bli8hwda	329	1	A	a	DT
cord-279463-bli8hwda	329	2	signal	signal	JJ
cord-279463-bli8hwda	329	3	sequence	sequence	NN
cord-279463-bli8hwda	329	4	for	for	IN
cord-279463-bli8hwda	329	5	the	the	DT
cord-279463-bli8hwda	329	6	insertion	insertion	NN
cord-279463-bli8hwda	329	7	of	of	IN
cord-279463-bli8hwda	329	8	a	a	DT
cord-279463-bli8hwda	329	9	transmembrane	transmembrane	NN
cord-279463-bli8hwda	329	10	glycoprotein	glycoprotein	NN
cord-279463-bli8hwda	329	11	.	.	.
cord-279463-bli8hwda	330	1	Similarities	similarity	NNS
cord-279463-bli8hwda	330	2	to	to	IN
cord-279463-bli8hwda	330	3	the	the	DT
cord-279463-bli8hwda	330	4	signals	signal	NNS
cord-279463-bli8hwda	330	5	of	of	IN
cord-279463-bli8hwda	330	6	secretory	secretory	JJ
cord-279463-bli8hwda	330	7	proteins	protein	NNS
cord-279463-bli8hwda	330	8	in	in	IN
cord-279463-bli8hwda	330	9	primary	primary	JJ
cord-279463-bli8hwda	330	10	structure	structure	NN
cord-279463-bli8hwda	330	11	and	and	CC
cord-279463-bli8hwda	330	12	function	function	NN
cord-279463-bli8hwda	330	13	.	.	.
cord-279463-bli8hwda	331	1	J.	J.	NNP
cord-279463-bli8hwda	331	2	Biol	Biol	NNP
cord-279463-bli8hwda	331	3	.	.	.
cord-279463-bli8hwda	332	1	Chem	Chem	NNP
cord-279463-bli8hwda	332	2	.	.	.
cord-279463-bli8hwda	333	1	253	253	CD
cord-279463-bli8hwda	333	2	,	,	,
cord-279463-bli8hwda	333	3	8667	8667	CD
cord-279463-bli8hwda	333	4	-	-	SYM
cord-279463-bli8hwda	333	5	8670.Lipp	8670.lipp	CD
cord-279463-bli8hwda	333	6	,	,	,
cord-279463-bli8hwda	333	7	J.	J.	NNP
cord-279463-bli8hwda	333	8	,	,	,
cord-279463-bli8hwda	333	9	and	and	CC
cord-279463-bli8hwda	333	10	Dobberstein	Dobberstein	NNP
cord-279463-bli8hwda	333	11	,	,	,
cord-279463-bli8hwda	333	12	B.	B.	NNP
cord-279463-bli8hwda	333	13	(	(	-LRB-
cord-279463-bli8hwda	333	14	1986	1986	CD
cord-279463-bli8hwda	333	15	)	)	-RRB-
cord-279463-bli8hwda	333	16	.	.	.
cord-279463-bli8hwda	334	1	Signal	signal	NN
cord-279463-bli8hwda	334	2	recognition	recognition	NN
cord-279463-bli8hwda	334	3	particle	particle	NN
cord-279463-bli8hwda	334	4	-	-	HYPH
cord-279463-bli8hwda	334	5	dependent	dependent	JJ
cord-279463-bli8hwda	334	6	membrane	membrane	NN
cord-279463-bli8hwda	334	7	insertion	insertion	NN
cord-279463-bli8hwda	334	8	of	of	IN
cord-279463-bli8hwda	334	9	mouse	mouse	NN
cord-279463-bli8hwda	334	10	invariant	invariant	JJ
cord-279463-bli8hwda	334	11	chain	chain	NN
cord-279463-bli8hwda	334	12	:	:	:
cord-279463-bli8hwda	334	13	a	a	DT
cord-279463-bli8hwda	334	14	membrane	membrane	NN
cord-279463-bli8hwda	334	15	spanning	span	VBG
cord-279463-bli8hwda	334	16	protein	protein	NN
cord-279463-bli8hwda	334	17	with	with	IN
cord-279463-bli8hwda	334	18	a	a	DT
cord-279463-bli8hwda	334	19	cytoplasmically	cytoplasmically	RB
cord-279463-bli8hwda	334	20	exposed	expose	VBN
cord-279463-bli8hwda	334	21	amino	amino	NN
cord-279463-bli8hwda	334	22	-	-	HYPH
cord-279463-bli8hwda	334	23	terminus	terminus	NN
cord-279463-bli8hwda	334	24	.	.	.
cord-279463-bli8hwda	335	1	J.	J.	NNP
cord-279463-bli8hwda	335	2	Cell	Cell	NNP
cord-279463-bli8hwda	335	3	Biol	Biol	NNP
cord-279463-bli8hwda	335	4	.	.	.
cord-279463-bli8hwda	336	1	702	702	CD
cord-279463-bli8hwda	336	2	,	,	,
cord-279463-bli8hwda	336	3	2169	2169	CD
cord-279463-bli8hwda	337	1	-2175	-2175	NNS
cord-279463-bli8hwda	337	2	.	.	.
cord-279463-bli8hwda	338	1	Long	long	RB
cord-279463-bli8hwda	338	2	,	,	,
cord-279463-bli8hwda	338	3	E.	E.	NNP
cord-279463-bli8hwda	338	4	0	0	CD
cord-279463-bli8hwda	338	5	.	.	.
cord-279463-bli8hwda	339	1	(	(	-LRB-
cord-279463-bli8hwda	339	2	1985	1985	CD
cord-279463-bli8hwda	339	3	.	.	.
cord-279463-bli8hwda	340	1	In	in	IN
cord-279463-bli8hwda	340	2	search	search	NN
cord-279463-bli8hwda	340	3	of	of	IN
cord-279463-bli8hwda	340	4	a	a	DT
cord-279463-bli8hwda	340	5	function	function	NN
cord-279463-bli8hwda	340	6	for	for	IN
cord-279463-bli8hwda	340	7	the	the	DT
cord-279463-bli8hwda	340	8	invariant	invariant	JJ
cord-279463-bli8hwda	340	9	chain	chain	NN
cord-279463-bli8hwda	340	10	associated	associate	VBN
cord-279463-bli8hwda	340	11	with	with	IN
cord-279463-bli8hwda	340	12	la	la	NNP
cord-279463-bli8hwda	340	13	antigens	antigen	NNS
cord-279463-bli8hwda	340	14	.	.	.
cord-279463-bli8hwda	341	1	Surv	Surv	NNP
cord-279463-bli8hwda	341	2	.	.	.
cord-279463-bli8hwda	342	1	Immunol	Immunol	NNP
cord-279463-bli8hwda	342	2	.	.	.
cord-279463-bli8hwda	343	1	Res	Res	NNP
cord-279463-bli8hwda	343	2	.	.	NNP
cord-279463-bli8hwda	343	3	4	4	CD
cord-279463-bli8hwda	343	4	,	,	,
cord-279463-bli8hwda	343	5	27	27	CD
cord-279463-bli8hwda	343	6	-	-	SYM
cord-279463-bli8hwda	343	7	34	34	CD
cord-279463-bli8hwda	343	8	.	.	.
cord-279463-bli8hwda	344	1	Lively	lively	RB
cord-279463-bli8hwda	344	2	,	,	,
cord-279463-bli8hwda	344	3	M.	M.	NNP
cord-279463-bli8hwda	344	4	O.	O.	NNP
cord-279463-bli8hwda	344	5	,	,	,
cord-279463-bli8hwda	344	6	and	and	CC
cord-279463-bli8hwda	344	7	Walsh	Walsh	NNP
cord-279463-bli8hwda	344	8	,	,	,
cord-279463-bli8hwda	344	9	K.	K.	NNP
cord-279463-bli8hwda	344	10	A.	A.	NNP
cord-279463-bli8hwda	344	11	(	(	-LRB-
cord-279463-bli8hwda	344	12	1983	1983	CD
cord-279463-bli8hwda	344	13	)	)	-RRB-
cord-279463-bli8hwda	345	1	Spiess	Spiess	NNP
cord-279463-bli8hwda	345	2	.	.	.
cord-279463-bli8hwda	346	1	M.	M.	NNP
cord-279463-bli8hwda	346	2	,	,	,
cord-279463-bli8hwda	346	3	and	and	CC
cord-279463-bli8hwda	346	4	Lodish	Lodish	NNP
cord-279463-bli8hwda	346	5	.	.	.
cord-279463-bli8hwda	347	1	H.	H.	NNP
cord-279463-bli8hwda	347	2	F.	F.	NNP
cord-279463-bli8hwda	347	3	(	(	-LRB-
cord-279463-bli8hwda	347	4	1986	1986	CD
cord-279463-bli8hwda	347	5	)	)	-RRB-
cord-279463-bli8hwda	347	6	.	.	.
cord-279463-bli8hwda	348	1	An	an	DT
cord-279463-bli8hwda	348	2	internal	internal	JJ
cord-279463-bli8hwda	348	3	signal	signal	NN
cord-279463-bli8hwda	348	4	sequence	sequence	NN
cord-279463-bli8hwda	348	5	:	:	:
cord-279463-bli8hwda	348	6	the	the	DT
cord-279463-bli8hwda	348	7	asialoglycoprotein	asialoglycoprotein	NNP
cord-279463-bli8hwda	348	8	receptor	receptor	NN
cord-279463-bli8hwda	348	9	membrane	membrane	NN
cord-279463-bli8hwda	348	10	anchor	anchor	NN
cord-279463-bli8hwda	348	11	.	.	.
cord-279463-bli8hwda	349	1	Cell	cell	NN
cord-279463-bli8hwda	349	2	44	44	CD
cord-279463-bli8hwda	349	3	,	,	,
cord-279463-bli8hwda	349	4	177	177	CD
cord-279463-bli8hwda	349	5	-	-	SYM
cord-279463-bli8hwda	349	6	165	165	CD
cord-279463-bli8hwda	349	7	.	.	.
cord-279463-bli8hwda	350	1	Stanley	Stanley	NNP
cord-279463-bli8hwda	350	2	,	,	,
cord-279463-bli8hwda	350	3	K.	K.	NNP
cord-279463-bli8hwda	350	4	K.	K.	NNP
cord-279463-bli8hwda	350	5	,	,	,
cord-279463-bli8hwda	350	6	and	and	CC
cord-279463-bli8hwda	350	7	Luzio	Luzio	NNP
cord-279463-bli8hwda	350	8	,	,	,
cord-279463-bli8hwda	350	9	J.	J.	NNP
cord-279463-bli8hwda	350	10	P	P	NNP
cord-279463-bli8hwda	350	11	(	(	-LRB-
cord-279463-bli8hwda	350	12	1984	1984	CD
cord-279463-bli8hwda	350	13	)	)	-RRB-
cord-279463-bli8hwda	350	14	.	.	.
cord-279463-bli8hwda	351	1	Construction	construction	NN
cord-279463-bli8hwda	351	2	of	of	IN
cord-279463-bli8hwda	351	3	a	a	DT
cord-279463-bli8hwda	351	4	new	new	JJ
cord-279463-bli8hwda	351	5	family	family	NN
cord-279463-bli8hwda	351	6	of	of	IN
cord-279463-bli8hwda	351	7	high	high	JJ
cord-279463-bli8hwda	351	8	efficiency	efficiency	NN
cord-279463-bli8hwda	351	9	bacterial	bacterial	JJ
cord-279463-bli8hwda	351	10	expression	expression	NN
cord-279463-bli8hwda	351	11	vectors	vector	NNS
cord-279463-bli8hwda	351	12	:	:	:
cord-279463-bli8hwda	351	13	identification	identification	NN
cord-279463-bli8hwda	351	14	of	of	IN
cord-279463-bli8hwda	351	15	cDNA	cdna	NN
cord-279463-bli8hwda	351	16	clones	clone	NNS
cord-279463-bli8hwda	351	17	coding	code	VBG
cord-279463-bli8hwda	351	18	for	for	IN
cord-279463-bli8hwda	351	19	human	human	JJ
cord-279463-bli8hwda	351	20	liver	liver	NN
cord-279463-bli8hwda	351	21	proteins	protein	NNS
cord-279463-bli8hwda	351	22	.	.	.
cord-279463-bli8hwda	352	1	EMBO	EMBO	NNP
cord-279463-bli8hwda	352	2	J.	J.	NNP
cord-279463-bli8hwda	352	3	3	3	CD
cord-279463-bli8hwda	352	4	,	,	,
cord-279463-bli8hwda	352	5	1429	1429	CD
cord-279463-bli8hwda	352	6	-1434	-1434	NFP
cord-279463-bli8hwda	352	7	.	.	.
cord-279463-bli8hwda	353	1	Strubin	Strubin	NNP
cord-279463-bli8hwda	353	2	,	,	,
cord-279463-bli8hwda	353	3	M.	M.	NNP
cord-279463-bli8hwda	353	4	,	,	,
cord-279463-bli8hwda	353	5	Mach	Mach	NNP
cord-279463-bli8hwda	353	6	,	,	,
cord-279463-bli8hwda	353	7	B.	B.	NNP
cord-279463-bli8hwda	353	8	,	,	,
cord-279463-bli8hwda	353	9	and	and	CC
cord-279463-bli8hwda	353	10	Long	Long	NNP
cord-279463-bli8hwda	353	11	,	,	,
cord-279463-bli8hwda	353	12	E.	E.	NNP
cord-279463-bli8hwda	353	13	0	0	CD
cord-279463-bli8hwda	353	14	.	.	.
cord-279463-bli8hwda	354	1	(	(	-LRB-
cord-279463-bli8hwda	354	2	1984	1984	CD
cord-279463-bli8hwda	354	3	.	.	.
cord-279463-bli8hwda	355	1	The	the	DT
cord-279463-bli8hwda	355	2	complete	complete	JJ
cord-279463-bli8hwda	355	3	sequence	sequence	NN
cord-279463-bli8hwda	355	4	of	of	IN
cord-279463-bli8hwda	355	5	the	the	DT
cord-279463-bli8hwda	355	6	mRNA	mrna	NN
cord-279463-bli8hwda	355	7	for	for	IN
cord-279463-bli8hwda	355	8	the	the	DT
cord-279463-bli8hwda	355	9	HLA	HLA	NNP
cord-279463-bli8hwda	355	10	-	-	HYPH
cord-279463-bli8hwda	355	11	DR	DR	NNP
cord-279463-bli8hwda	355	12	associated	associate	VBD
cord-279463-bli8hwda	355	13	invariant	invariant	JJ
cord-279463-bli8hwda	355	14	chain	chain	NN
cord-279463-bli8hwda	355	15	reveals	reveal	VBZ
cord-279463-bli8hwda	355	16	a	a	DT
cord-279463-bli8hwda	355	17	polypeptide	polypeptide	NN
cord-279463-bli8hwda	355	18	with	with	IN
cord-279463-bli8hwda	355	19	an	an	DT
cord-279463-bli8hwda	355	20	unusual	unusual	JJ
cord-279463-bli8hwda	355	21	transmembrane	transmembrane	NN
cord-279463-bli8hwda	355	22	polarity	polarity	NN
cord-279463-bli8hwda	355	23	.	.	.
cord-279463-bli8hwda	356	1	EMBO	EMBO	NNP
cord-279463-bli8hwda	356	2	J.	J.	NNP
cord-279463-bli8hwda	356	3	3,869~872	3,869~872	CD
cord-279463-bli8hwda	356	4	.	.	.
cord-279463-bli8hwda	356	5	 	 	_SP