id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-001381-b0tlco4t	Howie, Stephen R. C.	Etiology of Severe Childhood Pneumonia in The Gambia, West Africa, Determined by Conventional and Molecular Microbiological Analyses of Lung and Pleural Aspirate Samples	2014-09-01		.txt	text/plain	2360	123	46	title: Etiology of Severe Childhood Pneumonia in The Gambia, West Africa, Determined by Conventional and Molecular Microbiological Analyses of Lung and Pleural Aspirate Samples Molecular analyses of lung aspirates from Gambian children with severe pneumonia detected pathogens more frequently than did culture and showed a predominance of bacteria, principally Streptococcus pneumoniae, >75% being of serotypes covered by current pneumococcal conjugate vaccines. influenzae), 16S rRNA PCR, multilocus sequence typing (MLST), molecular serotyping, and multiplex fast-track 33 PCR, all using standard methods (Supplementary Appendix 2) [2, [6] [7] [8] [9] [10] [11] [12] [13] , were performed at the MRC Unit in The Gambia, and multiplex MassTag PCR [14] was performed at Columbia University. This study also confirms that molecular methods are able to detect potential pathogens far more readily than culture and have a role in defining the etiology of pneumonia.	./cache/cord-001381-b0tlco4t.txt	./txt/cord-001381-b0tlco4t.txt