id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-327862-zcg3baym	Luo, Yiqi Ruben	Kinetics of SARS-CoV-2 Antibody Avidity Maturation and Association with Disease Severity	2020-09-14		.txt	text/plain	1141	93	59	The kinetics of IgG avidity maturation during SARS-CoV-2 infection was studied. It was found that there was a strong correlation between IgG avidity and days since symptom onset, and peak readings were significantly higher in severe than mild disease cases. 3, 4 Here we report the development of a method to characterize SARS-CoV-2 IgG avidity maturation in COVID-19 patients from initial diagnosis through convalescence. The IgG avidity assay was established on a novel label-free immunoassay platform Gator Analyzer (Gator Bio, Palo Alto, CA) to measure SARS-CoV-2 IgG avidity to the virus spike protein receptor-binding domain (RBD). The signal increase in the final step, which is proportional to the quantity of RBD-IgG-Anti-IgG immune complex on the sensing probe, was measured. As other isotypes of antibodies might bind to RBD in the second step, the measurement of the RBD-IgG-Anti-IgG immune complex enhanced the assay specificity. Magnitude and kinetics of anti-SARS-CoV-2 antibody responses and their relationship to disease severity	./cache/cord-327862-zcg3baym.txt	./txt/cord-327862-zcg3baym.txt