id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-354943-wxhbwcfr	Guo, Li	Profiling Early Humoral Response to Diagnose Novel Coronavirus Disease (COVID-19)	2020-03-21		.txt	text/plain	3486	181	55	METHODS: The host humoral response against SARS-CoV-2, including IgA, IgM, and IgG response, was examined by using an ELISA-based assay on the recombinant viral nucleocapsid protein. The positive detection rate is significantly increased (98.6%) when combining IgM ELISA assay with PCR for each patient compared with a single qPCR test (51.9%). Western blot analysis showed that there was no cross-reactivity of SARS-CoV-2 rNP with human plasma positive for IgG antibodies against NL63, 229E, OC43, and HKU1. The antibody levels were then evaluated in the plasma samples of CCs and PCs. The appearance of IgM, IgA, and IgG antibodies against SARS-CoV-2 was positive as early as day 1 after the symptom onset ( Figure 3A) . These results suggest that IgM ELISA can increase the positive detection rate when combined with the PCR method and can be used for the early diagnosis of COVID-19 infections.	./cache/cord-354943-wxhbwcfr.txt	./txt/cord-354943-wxhbwcfr.txt