id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-006490-jo8dhtb0	Maillet, M.	Pneumocystis jirovecii (Pj) quantitative PCR to differentiate Pj pneumonia from Pj colonization in immunocompromised patients	2013-08-30		.txt	text/plain	2531	125	41	All positive Pj qPCR [targeting the major surface glycoprotein (MSG) gene] obtained in respiratory samples from immunocompromised patients presenting pneumonia at the Grenoble University Hospital, France, were collected between August 2009 and April 2011. qPCR targeting the MSG gene can be helpful to discriminate PCP from Pj colonization in immunocompromised patients, using two cut-off values, with a gray zone between them. The aim of this study was to use Pj real-time quantitative PCR (qPCR) in order to differentiate PCP and Pj colonization in immunocompromised patients. A receiver operating characteristic (ROC) curve for copy numbers was constructed and used to define cut-off values in order to discriminate the definite and probable PCP groups from the colonized group (possible PCP group and other diagnosis). In conclusion, our study shows that our qPCR targeting the MSG gene in respiratory samples can help discriminate PCP from pneumonia with Pj colonization in immunocompromised patients, using two cut-off values of 3,160 and 31,600 copies/ml, with a gray zone between them.	./cache/cord-006490-jo8dhtb0.txt	./txt/cord-006490-jo8dhtb0.txt