id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-266085-914y3je0	Correa, Isabel	Evaluation of Antigen-Conjugated Fluorescent Beads to Identify Antigen-Specific B Cells	2018-03-23		.txt	text/plain	6893	282	42	To establish this, we selected Folate Receptor alpha (FRα) as a model antigen and a mouse B cell line, expressing both the soluble and the membrane-bound forms of a human/mouse chimeric antibody (MOv18 IgG1) specific for FRα, as test antibody-expressing cells. In our study, we present an antigen-conjugated fluorescent bead methodology designed to identify and isolate single antibody-expressing cells and to derive and clone matched heavy and light chain antibody variable regions into full length antibodies. The workflow features bead-based identification and isolation of specific B cells using direct fluorescent-activated cell sorting, sequencing, and cloning of matched heavy and light chain variable regions in a single full sequence antibody expression vector system, and expression and testing the antigenic reactivity of the antibody clone. Following flow sorting of single cells directly into lysis buffer, we confirmed that it is possible to extract matched H and L chain variable region sequences from single antibody-expressing cells selected by specific antigen-coated beads.	./cache/cord-266085-914y3je0.txt	./txt/cord-266085-914y3je0.txt