id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-298604-tn8hc6jv	Khawaja, Akif A.	Identification of a Novel HIF-1α-α(M)β(2) Integrin-NET Axis in Fibrotic Interstitial Lung Disease	2020-10-15		.txt	text/plain	7971	420	40	Finally, we observed NET-like structures in IPF lung sections and in the BAL from ILD patients, and quantification showed increased cell-free DNA content (+5.5-fold, p < 0.01) and MPO-citrullinated histone H3 complexes (+21.9-fold, p < 0.01) in BAL from ILD patients compared to non-ILD controls. Finally, we observed NET-like structures in IPF lung sections and in the BAL from ILD patients, and quantification showed increased cell-free DNA content (+5.5-fold, p < 0.01) and MPO-citrullinated histone H3 complexes (+21.9-fold, p < 0.01) in BAL from ILD patients compared to non-ILD controls. We measured neutrophil adhesion to primary human endothelial cells in the absence or presence of PMA (a general integrin activator) or LPS (to mimic infectious stimuli), stimuli that induce NETs via distinct pathways (42) . We report, for the first time, that neutrophils and endothelial cells in ILD lung biopsies display HIF-1α expression and provide evidence of the extracellular release of nuclear DNA, citrullinated histones and MPO, indicative of NET formation in the ILD lung.	./cache/cord-298604-tn8hc6jv.txt	./txt/cord-298604-tn8hc6jv.txt