id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-260644-5moccf8c	Hashemi, Seyed Ahmad	Development of a PCR-RFLP method for detection of D614G mutation in SARS-CoV-2	2020-11-07		.txt	text/plain	2174	149	67	title: Development of a PCR-RFLP method for detection of D614G mutation in SARS-CoV-2 Regarding the high price and low availability of sequencing techniques in developing countries, here we describe a rapid and inexpensive method for the detection of D614G mutation in SARS-CoV-2. Some researchers evaluated and compared the whole genome sequence of SARS-CoV-2 isolated in various parts of the world and identified some mutations. The high-frequency mutations of the SARS-CoV-2 genome were seen in nsp6, RNA polymerase, helicase, membrane glycoprotein, RNA primase, nucleocapsid phosphoprotein, and spike protein genes (Yin, 2020) . In the first step, we used the sequence of S protein of SARS-CoV-2, published in Gene bank with accession number MT252819.1, for appropriate restriction endonuclease selection and primer design. The D614G mutation of SARS-CoV-2 spike protein enhances viral infectivity	./cache/cord-260644-5moccf8c.txt	./txt/cord-260644-5moccf8c.txt