id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-314640-vik5rgnq	Ohkubo, Kimihiro	Aminopeptidase activity in human nasal mucosa()()()()	2005-11-02		.txt	text/plain	4321	256	53	Several aminopeptidase (Ap) activities were defined by (1) substrate specificity with leucine-enkephalin (leu-Ap) and alanine-nitroanilide (ala-Ap), (2) inhibitor studies with puromycin and bestatin, (3) enzyme activity histochemistry (zymography), (4) immunohistochemistry, and (5) gel electrophoresis. To begin defining the physiology of aminopeptidases in airways, we have used multiple assays to characterize aminopeptidase activity in soluble and membrane fractions of human nasal mucosa. Enkephalin-degrading aminopeptidase activity was expressed as picomoles of leuenkephalin metabolized per minute per milligram of protein for extracts, or as picomoles of leu-enkephalin metabolized per minute per milliliter for nasal lavage fluid samples. Alanine aminopeptidase (Ala-Ap) assay samples (25 µL) were added to cuvettes containing 1.65 mL of 10 mmol/L tris HCl buffer (pH 7.4), 1 µmol/L phosphoramidon, and 10 µmol/L captopril, mixed thoroughly, and incubated for 1 minute.	./cache/cord-314640-vik5rgnq.txt	./txt/cord-314640-vik5rgnq.txt