id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-280350-ay4cnzn5	Chan, Jasper F.W.	Broad-spectrum antivirals for the emerging Middle East respiratory syndrome coronavirus	2013-10-03		.txt	text/plain	5156	259	45	We then assessed the anti-MERS-CoV activities of the identified compounds and of interferons, nelfinavir, and lopinavir because of their reported anti-coronavirus activities in terms of cytopathic effect inhibition, viral yield reduction, and plaque reduction assays in Biosafety Level-3 laboratory. Given the limited time available to develop novel anti-MERS-CoV agents in this evolving epidemic, we attempted to provide an alternative solution by identifying potential broad-spectrum antiviral agents against MERS-CoV and influenza A viruses by a small compound-based forward chemical genetics approach using chemical libraries consisting of 1280 drug compounds already marketed or having reached clinical trials in the United States, Europe, or Asia (Microsource Discovery Systems, USA). 25 We then assessed the anti-MERS-CoV activities of the identified drug compounds in cell culture by cytopathic effect (CPE) inhibition, viral yield reduction, and plaque reduction assay (PRA) assays, as well as drug cytotoxicity.	./cache/cord-280350-ay4cnzn5.txt	./txt/cord-280350-ay4cnzn5.txt