id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-337854-5ogip9tz	Huang, Wanqiu	The determination of release from isolation of COVID-19 patients requires ultra-high sensitivity nucleic acid test technology	2020-07-02		.txt	text/plain	1020	67	53	In our study, we developed an improved strategy, termed as nestRPA (nest recombinase polymerase amplification), which could greatly improve the sensitivity of nucleic acid detection for SARS-CoV-2 than RPA or qPCR. Using nestRPA technology, we found that positive plasmid containing SARS-CoV-2 with the concentration of 1 copy/ul could also be stably detected by Fragment 5 and nucleic acid detection results were negative using qPCR. Our results suggested that the ultra-sensitive nucleic acid detection technique has important implications for early identification of those asymptomatic carriers infected with SARS-CoV-2. In addition, many experts of COVID-19 prevention and treatment clearly pointed out that the inaccurate sample collection were also one of the important reasons for the false negative result of SARS-CoV-2 nucleic acid [6] [7] [8] . If all the links in the detection of SARS-CoV-2 nucleic acid could be strictly administrated, false negative could be completely eliminated, and the discontinuation of isolation will no longer be a dilemma for us.	./cache/cord-337854-5ogip9tz.txt	./txt/cord-337854-5ogip9tz.txt