id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-251974-2zwrqjj9	Geller, Chloé	A new Sephadex™-based method for removing microbicidal and cytotoxic residues when testing antiseptics against viruses: Experiments with a human coronavirus as a model	2009-04-05		.txt	text/plain	9395	630	63	The European Standard suggests other methods, based on gel-filtration techniques, using products as Sephadex TM LH-20, Microspin TM columns S400HR or Minicon ® concentrators, but they lead to problems of contact times, separation capacity and cost (supplementary data, Fig. 1 ). These tests were also performed with solutions of CHX and HXM at various concentrations and after their filtration on the "in-house" Sephadex TM columns to evaluate their retention and the elimination of potential cytotoxicity. Three types of controls were performed in each experiment to validate the results: (i) non-retention of viruses after filtration on Sephadex TM columns, (ii) neutralization of the potential antiviral activity of the product tested, and (iii) elimination of its cytotoxicity. To assess the non-retention of viruses, e.g. HCoV 229E, viral suspensions were mixed with sterile distilled water for the contact time defined for the experiment, filtered on the "in-house" Sephadex TM columns and titrated as describe above (Section 2.3).	./cache/cord-251974-2zwrqjj9.txt	./txt/cord-251974-2zwrqjj9.txt