id author title date pages extension mime words sentences flesch summary cache txt cord-257850-x7qtxaum Majchrzykiewicz-Koehorst, Joanna A. Rapid and generic identification of influenza A and other respiratory viruses with mass spectrometry 2015-03-01 .txt text/plain 6463 319 48 The development of an improved and rapid sample preparation method allowed generic and rapid identification of cultured respiratory viruses by mass spectrometry. In two independent experiments, all ten influenza strains were correctly identified as either H1N1 or H3N2 influenza A virus, with an identification limit of 7 × 10 6 genome copies in the total sample volume subjected to LC-MS/MS analysis (corresponding to a CT value of approximately 30-32; Table 3 ). At this viral titer, the influenza viruses were typed and subtyped based on the identification of peptides derived from the nucleoprotein protein, indicated in boldface in Table 4 . The identification limit at which the RSV strain was identified correctly, was 3.6 × 10 7 genome copy equivalents in the total sample volume subjected to LC-MS/MS analysis (corresponding to a CT value of approximately 28; Tables 5 and S1). ./cache/cord-257850-x7qtxaum.txt ./txt/cord-257850-x7qtxaum.txt