id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-279541-rjp2d1u9	Chutinimitkul, Salin	H5N1 Oseltamivir-resistance detection by real-time PCR using two high sensitivity labeled TaqMan probes	2006-10-19		.txt	text/plain	4114	211	50	Overall, the assay based on real-time PCR with two labeled TaqMan probes described here should be useful for detecting Oseltamivir-resistant H274Y H5N1 influenza A virus in many species and various sources of specimens with high sensitivity and specificity. The nucleotide sequences (N = 246) of the neuraminidase gene of influenza A virus (H5N1) were taken from the Genbank database going back as far as 2003-2006 and hence, comprising entries isolated from various species, such as avian, cats, dog, tigers, swine and humans, including DQ250165, the sequence of one Vietnamese Oseltamivir-resistant patient (A/Vietnam/CL2009/2005(H5N1)). In conclusion, real-time PCR using two labeled TaqMan probes provides a highly specific and sensitive method to detect the amino acid alteration at position 274 of the influenza A subtype H5N1 neuraminidase gene causing oseltamivir resistance.	./cache/cord-279541-rjp2d1u9.txt	./txt/cord-279541-rjp2d1u9.txt