id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-286117-m1rlmlun	Pearson, Morgan	High-throughput viral microneutralization method for feline coronavirus using image cytometry	2020-09-23		.txt	text/plain	4496	232	49	To address these challenges we developed a novel high-throughput viral microneutralization assay, with quantification of virus-infected cells performed in a plate-based image cytometer. The Celigo Image Cytometer has demonstrated automated enumeration of viral plaques, foci, and individual virus-infected cells in a 96-well microplates using bright field or fluorescence imaging in less than 5 or 10 min, respectively, which can significantly reduce the time required for counting and analysis as well as eliminate operator-to-operator variation (Ramos et al., 2019; Rosen et al., 2019; Viedma and Pickett, 2018) . Development of the image cytometry-based high-throughput FCoV infection detection method involved the optimization of key variables: host cell seeding density, fluorescent labeling, assay buffer, microplate surface coating, virus concentration, and incubation time. After the optimization of each parameter for the high-throughput virus-infected cell counting method, the image cytometer performed the neutralization assay at approximately 15 min per 96-well plate for simultaneously acquiring and analyzing whole well images in bright field and fluorescence, equivalent to ~9 sec per sample.	./cache/cord-286117-m1rlmlun.txt	./txt/cord-286117-m1rlmlun.txt