id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-290831-45cu8alm	Zheng, Yuan Zhi	Quantification of recombinant core-like particles of bluetongue virus using immunosorbent electron microscopy	1999-06-02		.txt	text/plain	2860	171	56	Immunosorbent electron microscopy was used to quantify recombinant baculovirus-generated bluetongue virus (BTV) core-like particles (CLP) in either purified preparations or lysates of recombinant baculovirus-infected cells. French and Roy (1990) constructed a dual-recombinant baculovirus containing genes that encode VP3 and VP7 and their expression in insect cell culture resulted in the synthesis of large quantities of VP3 and VP7 and the concomitant formation of core-like particles (CLP) that lack RNA. Ovine polyclonal antiserum was raised to core particles of BTV-1 isolated from virus-infected cells and purified by equilibrium density centrifugation in cesium chloride (personal communication, B.T. Eaton). The number of crude CLP per negative film was determined by the ISEM method and the concentration of particles calculated by multiplying the number of CLP by the capture efficiency. Serial dilutions of purified CLP were made in uninfected Sf9 cell lysates and 5 ml aliquots of each dilution added to grids coated with a 1:100 dilution of antibody 20E9.	./cache/cord-290831-45cu8alm.txt	./txt/cord-290831-45cu8alm.txt