id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-294454-uzfsv2df	Bellau-Pujol, S.	Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses	2005-02-24		.txt	text/plain	6064	334	51	Three multiplex hemi-nested RT-PCR assays were developed to detect simultaneously 12 RNA respiratory viruses: influenza viruses A, B and C, human respiratory syncytial virus (hRSV), human metapneumovirus (hMPV), parainfluenza virus types 1–4 (PIV-1, -2, -3 and -4), human coronavirus OC43 and 229E (HCoV) and rhinovirus (hRV). The overall sensitivity (98%), rapidity and enhanced efficiency of these multiplex hemi-nested RT-PCR assays suggest that they would be a significant improvement over conventional methods for the detection of a broad spectrum of respiratory viruses. The aim of this study was to develop rapid, sensitive and specific molecular methods for the detection of a large panel of respiratory RNA viruses that are more powerful than Co-infections hRV + PIV-3 1 hRV + PIV-1 1 hRV + PIV-1 + hMPV 1 hRV + hRSV + hMPV 1 hRV + influenza A virus 1 hRV + hRSV 2 Total no. Simultaneous detection of influenza A, B, and C viruses, respiratory syncytial virus, and adenoviruses in clinical samples by multiplex reverse transcription nested-PCR assay	./cache/cord-294454-uzfsv2df.txt	./txt/cord-294454-uzfsv2df.txt