id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-317462-nvrl0vyi	Song, Zhenhui	Morphogenesis and proliferative rule of porcine transmissible gastroenteritis virus in porcine intestinal epithelial cells	2016-09-28		.txt	text/plain	3364	183	55	To gain a better understanding of the replication, proliferation and infection characteristics of porcine transmissible gastroenteritis virus (TGEV) in porcine intestinal epithelial cells (IECs), this study established a cell model of IECs infected with the Chongqing (CQ) strain of TGEV. The results also showed that from 0 to 12 h after TGEV infection of porcine IECs, the intracellular viral RNA content did not change significantly. Thus, we developed an in vitro model based on porcine intestinal epithelial cells (IEC) infected with TGEV, and used transmission electron microscopy (TEM), indirect immunofluorescence assay (IFA) and real-time fluorescence quantitative PCR (FQ-PCR) to investigate the infection mechanism of TGEV. TGEV CQ strain was used to infect porcine IECs, and the supernatant and cells were collected at different time points for FQ-PCR. The one-step growth curve of TGEV in porcine IECs showed that the amount of viral RNA did not change significantly from 0 to 12 h post-infection, whereas Dai et al.	./cache/cord-317462-nvrl0vyi.txt	./txt/cord-317462-nvrl0vyi.txt