id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-324213-3uqlimov	Bolotin, S.	Development of a novel real-time reverse-transcriptase PCR method for the detection of H275Y positive influenza A H1N1 isolates	2009-01-30		.txt	text/plain	4059	191	50	To evaluate this method, 40 oseltamivir resistant and 61 oseltamivir sensitive H1N1 influenza isolates were tested using Sanger sequencing, which is the reference method for detection of resistance, pyrosequencing and the novel H275Y RT-PCR assay. To evaluate this method, 40 oseltamivir resistant and 61 oseltamivir sensitive H1N1 influenza isolates were tested using Sanger sequencing, which is the reference method for detection of resistance, pyrosequencing and the novel H275Y RT-PCR assay. To determine the limit of detection (LOD) of the H275Y and N1 control RT-PCR assay, serial ten-fold dilutions of nucleic acid from an oseltamivir-sensitive clinical influenza A/Brisbane/57/2007 H1N1 isolate were tested. This study evaluated the use of a novel H275Y RT-PCR assay for detection of H275Y-positive isolates in comparison to Sanger sequencing and pyrosequencing, and found that while all three methods may have a role in influenza diagnostic testing, the H275Y RT-PCR assay is a rapid and effective test for the detection of oseltamivir resistance through mutation at residue 275.	./cache/cord-324213-3uqlimov.txt	./txt/cord-324213-3uqlimov.txt