id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-334463-nvu5tqxb	Kim, Chonsaeng	Echovirus 7 Entry into Polarized Intestinal Epithelial Cells Requires Clathrin and Rab7	2012-04-10		.txt	text/plain	6207	325	48	We found that drugs, small interfering RNAs (siRNAs), and dominant negative mutants that target factors required for clathrin-mediated endocytosis, including clathrin and dynamin, inhibited both EV7 infection and internalization of virions from the cell surface. We previously (19) observed that, to infect polarized epithelium, CVB3 binds DAF on the cell surface, moves to the tight junction, and then enters the cell by an unusual mechanism that is independent of both dynamin and clathrin but which requires caveolin; contact with CAR in the tight junction leads to an essential conformational change in the CVB3 virion, but disruption of the capsid does not appear to occur until the virus has moved to an unidentified intracellular compartment. We had previously observed that CVB3 infection of Caco-2 cells occurs by a complex process that depends on caveolin but not dynamin-2, appears to be independent of clathrin-mediated endocytosis, and is inhibited by depletion of membrane cholesterol (19) .	./cache/cord-334463-nvu5tqxb.txt	./txt/cord-334463-nvu5tqxb.txt