id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-012391-53hgrs40	Miazga-Karska, Malgorzata	Anti-Acne Action of Peptides Isolated from Burdock Root—Preliminary Studies and Pilot Testing	2020-04-27		.txt	text/plain	6451	354	51	The fraction of 46 Br-p peptides isolated from burdock root with a molecular weight below 5000 Da and theoretic pI (isoelectric point) of 3.67–11.83 showed a narrow spectrum of activity against Gram-positive acne bacterial strains. To sum up: preliminary biological studies confirmed the anti-acne properties of the isolated peptide fraction from burdock root and pointed to the possibility of using it to create an active dressing on the skin. The burdock roots samples, isolated using ultrasound and salting out procedure, were fractionated using a Sephadex G-50 gel filtration (Materials and Methods, Section 4.1.) The obtained fractions were evaluated for the amount of protein and antibacterial properties (the diffusion test in solid medium). Br-f was initially tested for antibacterial activity (Figure 1, Table 1 ), and the remaining Br-f part was further ultrafiltrated in Amicon (10 kDa cut-off), followed by freeze-drying to obtain final Br-p peptide samples (the protein content was 51 µg/mL).	./cache/cord-012391-53hgrs40.txt	./txt/cord-012391-53hgrs40.txt