id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-335377-zrbn637z	Ishimaru, Daniella	RNA dimerization plays a role in ribosomal frameshifting of the SARS coronavirus	2012-12-26		.txt	text/plain	7699	376	52	Furthermore, the inability to dimerize caused by the silent codon change in Stem 3 of SARS-CoV changed the viral growth kinetics and affected the levels of genomic and subgenomic RNA in infected cells. We further show that kissing dimer formation plays a role in frameshift-stimulation and modulates the relative abundance of full-length and subgenomic viral RNAs. Plasmids containing wild-type pseudoknot as well as the ÁS3 pk mutant were described in Plant et al (1) . Our previous NMR analysis of exchangeable imino protons of the SARS-CoV pseudoknot ( Figure 1A , wild-type pk) provided unequivocal evidence for the existence of Stem 3 (1). Surprisingly, in the context of the SARS-CoV Stem 3 sequence, 5 0 -cuug-3 0 tetraloop-capped mutants readily formed extended duplex structures as revealed by native gel and NMR analysis.	./cache/cord-335377-zrbn637z.txt	./txt/cord-335377-zrbn637z.txt