id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-334907-l4jjb93l	Ojeda, Nicolás	Interaction of the Amino-Terminal Domain of the ISAV Fusion Protein with a Cognate Cell Receptor	2020-05-27		.txt	text/plain	10687	487	48	These results demonstrate the important role of viral surface proteins in the regulation of infectivity; in particular, they may further define the influence of HPR on the fusion activity in ISAV, suggesting a novel role for F (i.e., receptor interaction). To assess the role of the F 1 domain on the fusion activity of F, different ISAV HE and F combinations were evaluated in membrane fusion assays, using transfected CHSE/F cells expressing the viral HE and F proteins and R18-labeled salmon RBCs. After adhesion, cells were subjected to trypsin treatment and low pH to activate the fusion mechanism. In ISAV, if the primary receptor (5N-4O sialic acids) binding via HE is not affected by the HPR length/esterase activity, possibly a secondary viral protein-cellular ligand interaction is regulated by those elements.	./cache/cord-334907-l4jjb93l.txt	./txt/cord-334907-l4jjb93l.txt