id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-000625-cpjlzutk	Ablordey, Anthony	Detection of Mycobacterium ulcerans by the Loop Mediated Isothermal Amplification Method	2012-04-03		.txt	text/plain	3673	184	47	In order to develop a field applicable technique that offers high detection sensitivity and specificity for the diagnosis of BU, we explored the use of the pocket warmer LAMP (pwLAMP) technique, a DNA amplification method using isothermal conditions (60uC) provided by a disposable pocket warmer [34] . In order to develop a simple and rapid test that can be used to diagnose Buruli ulcer under field conditions, we modified the conventional LAMP assay by using a disposable pocket warmer as a heating device for generating a constant temperature for the test reaction and employed the use of crude sample preparations consisting of boiled and unboiled extracts of the clinical specimen instead of using purified DNA as the diagnostic specimen. Thirty clinical specimens from suspected Buruli ulcer patients were investigated by the modified LAMP (or pocket warmer LAMP) and the conventional LAMP, as well as IS2404 PCR, a reference method for the detection of Mycobacterium ulcerans.	./cache/cord-000625-cpjlzutk.txt	./txt/cord-000625-cpjlzutk.txt