id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-001484-va0teako	Ahmed, Sarah A.	Rapid Identification of Black Grain Eumycetoma Causative Agents Using Rolling Circle Amplification	2014-12-04		.txt	text/plain	2922	165	48	A rapid, simple, and highly efficient molecular based method for identification of agents of black grain eumycetoma is introduced, aiming to improve diagnostic in endemic areas. Rolling circle amplification (RCA) is a powerful diagnostic method based on detection of specific nucleic-acid sequences and enzymatic amplification of circularized oligonucleotide probes under isothermal conditions [10] . The specificity of the 8 RCA probes was tested using strains of black-grain mycetoma causative species listed in table 1. Identification of mycetoma agent with phenotypic features is too limited, and physiological and biochemical techniques are laborious, time-consuming and nonspecific, whereas the currently available molecular methods based on DNA sequencing are specific but extremely expensive. We describe rolling circle amplification method for identification of black grain eumycetoma using species-specific padlock probes. In the present study we developed a simple, fast and highly specific molecular method for the identification of agents of black grain mycetoma.	./cache/cord-001484-va0teako.txt	./txt/cord-001484-va0teako.txt