id author title date pages extension mime words sentences flesch summary cache txt cord-001541-5d64esp4 Walker, Peter J. Evolution of Genome Size and Complexity in the Rhabdoviridae 2015-02-13 .txt text/plain 9157 398 45 We demonstrate that remarkable changes in genome size and complexity have occurred in rhabdoviruses in a clade-specific manner, primarily by extension and insertion of additional transcriptional units in the structural protein gene junctions, followed by occasional losses. All rhabdovirus genomes contained the five canonical structural protein genes (N, P, M, G and L); however, there was remarkable diversity in the number and location of other long ORFs. Across the data set, we identified 179 additional ORFs 180 nt in length of which 142 shared no detectable protein sequence similarity with any other protein in our data set or with those in public databases (S2 Table) . Interestingly, although substantial variation in the length of gene junctions was observed in several genera (including ephemeroviruses and lyssaviruses), most variation in genome size occurred as the result of the presence of new, non-canonical ORFs in the regions between the structural protein genes (Table 1) . ./cache/cord-001541-5d64esp4.txt ./txt/cord-001541-5d64esp4.txt