id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-265947-7j9qqi8w	Du, Wenjuan	The 2(nd) sialic acid-binding site of influenza A virus neuraminidase is an important determinant of the hemagglutinin-neuraminidase-receptor balance	2019-06-10		.txt	text/plain	9564	464	50	NAs of avian, but not human viruses, contain a functional 2(nd) sialic acid (SIA)-binding site (2SBS) adjacent to the catalytic site, which contributes to sialidase activity against multivalent substrates. Using a recently established BLI-based kinetic binding assay [37] an enhanced initial binding rate to 3'SLNLN but not 6'SLNLN ( Fig 4A and 4B ), was observed for hH3aN2 virus containing Role for 2 nd SIA-binding site of IAV NA in HA-NA-receptor balance a functional 2SBS in comparison to hH3hN2. We therefore studied the effect of the 2SBS in NA when combined with an avian-type Role for 2 nd SIA-binding site of IAV NA in HA-NA-receptor balance HA preferring binding to α2,3-linked SIAs. We generated the corresponding recombinant A/ Hong Kong/1/68 (H3N2) viruses containing 7 amino acid substitutions in the HA (see S8A Fig) .	./cache/cord-265947-7j9qqi8w.txt	./txt/cord-265947-7j9qqi8w.txt