id author title date pages extension mime words sentences flesch summary cache txt cord-269466-9hnal9ad Agbeci, Maxime Contribution of Host Intracellular Transport Machineries to Intercellular Movement of Turnip Mosaic Virus 2013-10-03 .txt text/plain 7184 391 49 In this study, we used a novel dual gene cassette construct that differentiated primary infected cells from cells infected after virus intercellular movement to show that the early as well as the late secretory pathway, but not endocytosis, was important for TuMV transport. By using a dual cassette of genes encoding fluorescent proteins that can differentiate between primary infected cells and cells infected after intercellular transport, we provide evidence that turnip mosaic virus (TuMV) needs a functional secretory pathway where pre-and post-Golgi trafficking and the actomyosin network are important for its movement. Fig. 2E shows that that there was no significant difference in the ratio of red over green fluorescence during BFA and CMA treatments compared with the no inhibitor treatment (TuMV alone) at 4 dpinf, indicating that viral protein production in the primary infected cells was not affected by the drug treatments. ./cache/cord-269466-9hnal9ad.txt ./txt/cord-269466-9hnal9ad.txt