id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-314451-mqnqjn0c	Roberts, Anjeanette	A Mouse-Adapted SARS-Coronavirus Causes Disease and Mortality in BALB/c Mice	2007-01-12		.txt	text/plain	9794	433	48	To generate a model that satisfies these criteria, we have serially passaged SARS-CoV in the respiratory tract of young BALB/c mice, resulting in a lethal virus that causes dosedependent weight loss and mortality associated with higher viral titers in the respiratory tract than are seen with the wildtype virus and with histopathologic findings of severe pulmonary disease. Northern blot analysis of RNA from infected Vero E6 cells indicated that genomic vRNA and viral mRNA and all eight sub-genomic mRNAs were present in similar ratios for the recombinant viruses and MA15 virus as for SARS-CoV (Urbani) ( Figure 2A ). In order to evaluate whether changes in tissue tropism or levels of viral replication could contribute to the lethal phenotype of the MA15 virus, viral titers in lungs, spleen, liver, and brain of BALB/c mice were determined at various time points following intranasal inoculation with SARS-CoV (Urbani) or MA15.	./cache/cord-314451-mqnqjn0c.txt	./txt/cord-314451-mqnqjn0c.txt