id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-324928-cpryxa6p	Lello, Laura Sandra	Cross-utilisation of template RNAs by alphavirus replicases	2020-09-04		.txt	text/plain	13762	646	50	The differences between outgroup viruses and those belonging to the SFV complex had an impact on the design of the template RNAs. The 5' region of the CHIKV genome contains seven SL structures that affect genome replication in mammalian and/or mosquito cells [33] . Cross-utilisation of template RNAs by alphavirus replicases structures can be predicted for RNAs of other members of the SFV complex (S1 Fig), the 5' ends of the template RNAs of SFV, MAYV, RRV and ONNV had an identical design to that of the previously constructed CHIKV template, i.e. the 5' UTR was followed by 231nt encoding the N-terminus of nsP1 [43] . The levels of Fluc and Gluc expression in human cells, transfected with plasmids expressing template RNA and corresponding polymerase negative control replicase (P1234 GAA ), were similar for trans-replicases derived from different viruses and the same was observed for Ae albopictus cells.	./cache/cord-324928-cpryxa6p.txt	./txt/cord-324928-cpryxa6p.txt