id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-342825-s8ddek2f	Kim, Ye Ji	Consecutive Inhibition of ISG15 Expression and ISGylation by Cytomegalovirus Regulators	2016-08-26		.txt	text/plain	10236	535	49	RNA interference of UBE1L (E1), UbcH8 (E2), Herc5 (E3), and UBP43 (ISG15 protease) revealed that ISGylation inhibits HCMV growth by downregulating viral gene expression and virion release in a manner that is more prominent at low multiplicity of infection. This result suggests that an indirect effect of virus infection on neighboring uninfected cells is responsible for the greater induction of ISG15 and protein ISGylation by HCMV than by UV-HCMV at low MOIs. Since HCMV IE1 inhibits the activation of ISRE-containing promoters by sequestering STAT2 [58] [59] [60] and PML [61] , IE1 expression may be responsible for the suppression of free ISG15 and ISG15 conjugate levels during HCMV infection. In addition to IE1, the presence of additional viral functions that downregulate protein ISGylation was prompted by our observation that UV-HCMV infection resulted in a higher level of ISG15 conjugates than IE1-deleted mutant virus at late times (Fig 2B) .	./cache/cord-342825-s8ddek2f.txt	./txt/cord-342825-s8ddek2f.txt