id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-348799-qu4zin3o	Wu, Nannan	The interferon stimulated gene 20 protein (ISG20) is an innate defense antiviral factor that discriminates self versus non-self translation	2019-10-10		.txt	text/plain	11391	520	48	This lack of specificity was not present in cells however, given that the ectopic expression of ISG20 did not lead to the indiscriminate degradation of cellular RNAs (ribosomal as well as small nucleolar RNAs previously shown to be associated to ISG20 [2] ), nor of an Hepatitis C virus (HCV)-derived Luciferase reporter mRNA produced in vitro and then transfected into cells (S4C and S4D Fig) , suggesting that in cells the RNase activity of ISG20 is tightly controlled. Self mimicry allows the escape of target genes' mRNAs from the effects of ISG20 VSV infection and ectopic DNA or RNA transfection do not bear much in common apart from the fact that both represent artificial injections into the cell of non-self genetic material from without. To further determine whether ISG20 acted on translation initiation and more specifically through specific initiation factors (eIFs), capped and polyadenylated mRNAs coding luciferase under the control of several 5' untranslated regions (5' UTRs) were generated in vitro and directly transfected in ISG20-expressing cells (Fig 3D) .	./cache/cord-348799-qu4zin3o.txt	./txt/cord-348799-qu4zin3o.txt