id sid tid token lemma pos cord-001257-t21l6i3f 1 1 key key JJ cord-001257-t21l6i3f 1 2 : : : cord-001257-t21l6i3f 1 3 cord-001257-t21l6i3f cord-001257-t21l6i3f NN cord-001257-t21l6i3f 1 4 authors author NNS cord-001257-t21l6i3f 1 5 : : : cord-001257-t21l6i3f 1 6 Kovalev Kovalev NNP cord-001257-t21l6i3f 1 7 , , , cord-001257-t21l6i3f 1 8 Nikolay Nikolay NNP cord-001257-t21l6i3f 1 9 ; ; : cord-001257-t21l6i3f 1 10 Nagy Nagy NNP cord-001257-t21l6i3f 1 11 , , , cord-001257-t21l6i3f 1 12 Peter Peter NNP cord-001257-t21l6i3f 1 13 D. D. NNP cord-001257-t21l6i3f 1 14 title title NN cord-001257-t21l6i3f 1 15 : : : cord-001257-t21l6i3f 1 16 The the DT cord-001257-t21l6i3f 1 17 Expanding Expanding NNP cord-001257-t21l6i3f 1 18 Functions Functions NNPS cord-001257-t21l6i3f 1 19 of of IN cord-001257-t21l6i3f 1 20 Cellular Cellular NNP cord-001257-t21l6i3f 1 21 Helicases Helicases NNP cord-001257-t21l6i3f 1 22 : : : cord-001257-t21l6i3f 2 1 The the DT cord-001257-t21l6i3f 2 2 Tombusvirus Tombusvirus NNP cord-001257-t21l6i3f 2 3 RNA RNA NNP cord-001257-t21l6i3f 2 4 Replication Replication NNP cord-001257-t21l6i3f 2 5 Enhancer Enhancer NNP cord-001257-t21l6i3f 2 6 Co Co NNP cord-001257-t21l6i3f 2 7 - - NN cord-001257-t21l6i3f 2 8 opts opt VBZ cord-001257-t21l6i3f 2 9 the the DT cord-001257-t21l6i3f 2 10 Plant Plant NNP cord-001257-t21l6i3f 2 11 eIF4AIII eif4aiii RBR cord-001257-t21l6i3f 2 12 - - : cord-001257-t21l6i3f 2 13 Like like JJ cord-001257-t21l6i3f 2 14 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 2 15 and and CC cord-001257-t21l6i3f 2 16 the the DT cord-001257-t21l6i3f 2 17 DDX5-Like ddx5-like JJ cord-001257-t21l6i3f 2 18 AtRH5 AtRH5 NNP cord-001257-t21l6i3f 2 19 DEAD DEAD NNP cord-001257-t21l6i3f 2 20 - - HYPH cord-001257-t21l6i3f 2 21 Box Box NNP cord-001257-t21l6i3f 2 22 RNA RNA NNP cord-001257-t21l6i3f 2 23 Helicases Helicases NNP cord-001257-t21l6i3f 2 24 to to TO cord-001257-t21l6i3f 2 25 Promote promote VB cord-001257-t21l6i3f 2 26 Viral Viral NNP cord-001257-t21l6i3f 2 27 Asymmetric Asymmetric NNP cord-001257-t21l6i3f 2 28 RNA RNA NNP cord-001257-t21l6i3f 2 29 Replication Replication NNP cord-001257-t21l6i3f 2 30 date date NN cord-001257-t21l6i3f 3 1 : : : cord-001257-t21l6i3f 3 2 2014 2014 CD cord-001257-t21l6i3f 3 3 - - HYPH cord-001257-t21l6i3f 3 4 04 04 CD cord-001257-t21l6i3f 3 5 - - SYM cord-001257-t21l6i3f 3 6 17 17 CD cord-001257-t21l6i3f 3 7 journal journal NN cord-001257-t21l6i3f 3 8 : : : cord-001257-t21l6i3f 4 1 PLoS PLoS NNP cord-001257-t21l6i3f 4 2 Pathog Pathog NNP cord-001257-t21l6i3f 4 3 DOI DOI NNP cord-001257-t21l6i3f 4 4 : : : cord-001257-t21l6i3f 5 1 10.1371 10.1371 CD cord-001257-t21l6i3f 5 2 / / SYM cord-001257-t21l6i3f 5 3 journal.ppat.1004051 journal.ppat.1004051 NNP cord-001257-t21l6i3f 5 4 sha sha NNP cord-001257-t21l6i3f 5 5 : : : cord-001257-t21l6i3f 5 6 5534fcf5c7e7df182da3c253d2312cd5662259b8 5534fcf5c7e7df182da3c253d2312cd5662259b8 CD cord-001257-t21l6i3f 5 7 doc_id doc_id CD cord-001257-t21l6i3f 5 8 : : : cord-001257-t21l6i3f 5 9 1257 1257 CD cord-001257-t21l6i3f 5 10 cord_uid cord_uid FW cord-001257-t21l6i3f 5 11 : : : cord-001257-t21l6i3f 6 1 t21l6i3f t21l6i3f NN cord-001257-t21l6i3f 6 2 Replication replication NN cord-001257-t21l6i3f 6 3 of of IN cord-001257-t21l6i3f 6 4 plus plus JJ cord-001257-t21l6i3f 6 5 - - HYPH cord-001257-t21l6i3f 6 6 strand strand NN cord-001257-t21l6i3f 6 7 RNA RNA NNP cord-001257-t21l6i3f 6 8 viruses virus NNS cord-001257-t21l6i3f 6 9 depends depend VBZ cord-001257-t21l6i3f 6 10 on on IN cord-001257-t21l6i3f 6 11 recruited recruit VBN cord-001257-t21l6i3f 6 12 host host NN cord-001257-t21l6i3f 6 13 factors factor NNS cord-001257-t21l6i3f 6 14 that that WDT cord-001257-t21l6i3f 6 15 aid aid VBP cord-001257-t21l6i3f 6 16 several several JJ cord-001257-t21l6i3f 6 17 critical critical JJ cord-001257-t21l6i3f 6 18 steps step NNS cord-001257-t21l6i3f 6 19 during during IN cord-001257-t21l6i3f 6 20 replication replication NN cord-001257-t21l6i3f 6 21 . . . cord-001257-t21l6i3f 7 1 Several several JJ cord-001257-t21l6i3f 7 2 of of IN cord-001257-t21l6i3f 7 3 the the DT cord-001257-t21l6i3f 7 4 co co NN cord-001257-t21l6i3f 7 5 - - JJ cord-001257-t21l6i3f 7 6 opted opt VBN cord-001257-t21l6i3f 7 7 host host NN cord-001257-t21l6i3f 7 8 factors factor NNS cord-001257-t21l6i3f 7 9 bind bind VBP cord-001257-t21l6i3f 7 10 to to IN cord-001257-t21l6i3f 7 11 the the DT cord-001257-t21l6i3f 7 12 viral viral JJ cord-001257-t21l6i3f 7 13 RNA RNA NNP cord-001257-t21l6i3f 7 14 , , , cord-001257-t21l6i3f 7 15 which which WDT cord-001257-t21l6i3f 7 16 plays play VBZ cord-001257-t21l6i3f 7 17 multiple multiple JJ cord-001257-t21l6i3f 7 18 roles role NNS cord-001257-t21l6i3f 7 19 , , , cord-001257-t21l6i3f 7 20 including include VBG cord-001257-t21l6i3f 7 21 mRNA mrna NN cord-001257-t21l6i3f 7 22 function function NN cord-001257-t21l6i3f 7 23 , , , cord-001257-t21l6i3f 7 24 as as IN cord-001257-t21l6i3f 7 25 an an DT cord-001257-t21l6i3f 7 26 assembly assembly NN cord-001257-t21l6i3f 7 27 platform platform NN cord-001257-t21l6i3f 7 28 for for IN cord-001257-t21l6i3f 7 29 the the DT cord-001257-t21l6i3f 7 30 viral viral JJ cord-001257-t21l6i3f 7 31 replicase replicase NN cord-001257-t21l6i3f 7 32 ( ( -LRB- cord-001257-t21l6i3f 7 33 VRC VRC NNP cord-001257-t21l6i3f 7 34 ) ) -RRB- cord-001257-t21l6i3f 7 35 , , , cord-001257-t21l6i3f 7 36 template template NN cord-001257-t21l6i3f 7 37 for for IN cord-001257-t21l6i3f 7 38 RNA RNA NNP cord-001257-t21l6i3f 7 39 synthesis synthesis NN cord-001257-t21l6i3f 7 40 , , , cord-001257-t21l6i3f 7 41 and and CC cord-001257-t21l6i3f 7 42 encapsidation encapsidation NN cord-001257-t21l6i3f 7 43 during during IN cord-001257-t21l6i3f 7 44 infection infection NN cord-001257-t21l6i3f 7 45 . . . cord-001257-t21l6i3f 8 1 It -PRON- PRP cord-001257-t21l6i3f 8 2 is be VBZ cord-001257-t21l6i3f 8 3 likely likely JJ cord-001257-t21l6i3f 8 4 that that IN cord-001257-t21l6i3f 8 5 remodeling remodeling NN cord-001257-t21l6i3f 8 6 of of IN cord-001257-t21l6i3f 8 7 the the DT cord-001257-t21l6i3f 8 8 viral viral JJ cord-001257-t21l6i3f 8 9 RNAs rna NNS cord-001257-t21l6i3f 8 10 and and CC cord-001257-t21l6i3f 8 11 RNA RNA NNP cord-001257-t21l6i3f 8 12 - - HYPH cord-001257-t21l6i3f 8 13 protein protein NN cord-001257-t21l6i3f 8 14 complexes complex NNS cord-001257-t21l6i3f 8 15 during during IN cord-001257-t21l6i3f 8 16 the the DT cord-001257-t21l6i3f 8 17 switch switch NN cord-001257-t21l6i3f 8 18 from from IN cord-001257-t21l6i3f 8 19 one one CD cord-001257-t21l6i3f 8 20 step step NN cord-001257-t21l6i3f 8 21 to to IN cord-001257-t21l6i3f 8 22 another another DT cord-001257-t21l6i3f 8 23 requires require VBZ cord-001257-t21l6i3f 8 24 RNA RNA NNP cord-001257-t21l6i3f 8 25 helicases helicase NNS cord-001257-t21l6i3f 8 26 . . . cord-001257-t21l6i3f 9 1 In in IN cord-001257-t21l6i3f 9 2 this this DT cord-001257-t21l6i3f 9 3 paper paper NN cord-001257-t21l6i3f 9 4 , , , cord-001257-t21l6i3f 9 5 we -PRON- PRP cord-001257-t21l6i3f 9 6 have have VBP cord-001257-t21l6i3f 9 7 discovered discover VBN cord-001257-t21l6i3f 9 8 a a DT cord-001257-t21l6i3f 9 9 second second JJ cord-001257-t21l6i3f 9 10 group group NN cord-001257-t21l6i3f 9 11 of of IN cord-001257-t21l6i3f 9 12 cellular cellular JJ cord-001257-t21l6i3f 9 13 RNA RNA NNP cord-001257-t21l6i3f 9 14 helicases helicase NNS cord-001257-t21l6i3f 9 15 , , , cord-001257-t21l6i3f 9 16 including include VBG cord-001257-t21l6i3f 9 17 the the DT cord-001257-t21l6i3f 9 18 eIF4AIII eIF4AIII NNS cord-001257-t21l6i3f 9 19 - - HYPH cord-001257-t21l6i3f 9 20 like like JJ cord-001257-t21l6i3f 9 21 yeast yeast NN cord-001257-t21l6i3f 9 22 Fal1p fal1p NN cord-001257-t21l6i3f 9 23 and and CC cord-001257-t21l6i3f 9 24 the the DT cord-001257-t21l6i3f 9 25 DDX5-like DDX5-like NNP cord-001257-t21l6i3f 9 26 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 9 27 and and CC cord-001257-t21l6i3f 9 28 the the DT cord-001257-t21l6i3f 9 29 orthologous orthologous JJ cord-001257-t21l6i3f 9 30 plant plant NN cord-001257-t21l6i3f 9 31 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 9 32 and and CC cord-001257-t21l6i3f 9 33 AtRH5 atrh5 RB cord-001257-t21l6i3f 9 34 DEAD dead JJ cord-001257-t21l6i3f 9 35 box box NN cord-001257-t21l6i3f 9 36 helicases helicase NNS cord-001257-t21l6i3f 9 37 , , , cord-001257-t21l6i3f 9 38 which which WDT cord-001257-t21l6i3f 9 39 are be VBP cord-001257-t21l6i3f 9 40 co co VBN cord-001257-t21l6i3f 9 41 - - VBN cord-001257-t21l6i3f 9 42 opted opt VBN cord-001257-t21l6i3f 9 43 by by IN cord-001257-t21l6i3f 9 44 tombusviruses tombusviruse NNS cord-001257-t21l6i3f 9 45 . . . cord-001257-t21l6i3f 10 1 Unlike unlike IN cord-001257-t21l6i3f 10 2 the the DT cord-001257-t21l6i3f 10 3 previously previously RB cord-001257-t21l6i3f 10 4 characterized characterize VBN cord-001257-t21l6i3f 10 5 DDX3-like DDX3-like NNP cord-001257-t21l6i3f 10 6 AtRH20 atrh20 NN cord-001257-t21l6i3f 10 7 / / SYM cord-001257-t21l6i3f 10 8 Ded1p ded1p IN cord-001257-t21l6i3f 10 9 helicases helicase NNS cord-001257-t21l6i3f 10 10 that that WDT cord-001257-t21l6i3f 10 11 bind bind VBP cord-001257-t21l6i3f 10 12 to to IN cord-001257-t21l6i3f 10 13 the the DT cord-001257-t21l6i3f 10 14 3′ 3′ CD cord-001257-t21l6i3f 10 15 terminal terminal NN cord-001257-t21l6i3f 10 16 promoter promoter NN cord-001257-t21l6i3f 10 17 region region NN cord-001257-t21l6i3f 10 18 in in IN cord-001257-t21l6i3f 10 19 the the DT cord-001257-t21l6i3f 10 20 viral viral JJ cord-001257-t21l6i3f 10 21 minus minus JJ cord-001257-t21l6i3f 10 22 - - HYPH cord-001257-t21l6i3f 10 23 strand strand NN cord-001257-t21l6i3f 10 24 ( ( -LRB- cord-001257-t21l6i3f 10 25 −)RNA −)RNA NNP cord-001257-t21l6i3f 10 26 , , , cord-001257-t21l6i3f 10 27 the the DT cord-001257-t21l6i3f 10 28 other other JJ cord-001257-t21l6i3f 10 29 class class NN cord-001257-t21l6i3f 10 30 of of IN cord-001257-t21l6i3f 10 31 eIF4AIII eIF4AIII NNPS cord-001257-t21l6i3f 10 32 - - : cord-001257-t21l6i3f 10 33 like like JJ cord-001257-t21l6i3f 10 34 RNA RNA NNP cord-001257-t21l6i3f 10 35 helicases helicase NNS cord-001257-t21l6i3f 10 36 bind bind VBP cord-001257-t21l6i3f 10 37 to to IN cord-001257-t21l6i3f 10 38 a a DT cord-001257-t21l6i3f 10 39 different different JJ cord-001257-t21l6i3f 10 40 cis cis NN cord-001257-t21l6i3f 10 41 - - HYPH cord-001257-t21l6i3f 10 42 acting act VBG cord-001257-t21l6i3f 10 43 element element NN cord-001257-t21l6i3f 10 44 , , , cord-001257-t21l6i3f 10 45 namely namely RB cord-001257-t21l6i3f 10 46 the the DT cord-001257-t21l6i3f 10 47 5′ 5′ CD cord-001257-t21l6i3f 10 48 proximal proximal JJ cord-001257-t21l6i3f 10 49 RIII(− riii(− NN cord-001257-t21l6i3f 10 50 ) ) -RRB- cord-001257-t21l6i3f 10 51 replication replication NN cord-001257-t21l6i3f 10 52 enhancer enhancer NN cord-001257-t21l6i3f 10 53 ( ( -LRB- cord-001257-t21l6i3f 10 54 REN REN NNP cord-001257-t21l6i3f 10 55 ) ) -RRB- cord-001257-t21l6i3f 10 56 element element NN cord-001257-t21l6i3f 10 57 in in IN cord-001257-t21l6i3f 10 58 the the DT cord-001257-t21l6i3f 10 59 TBSV TBSV NNP cord-001257-t21l6i3f 10 60 ( ( -LRB- cord-001257-t21l6i3f 10 61 −)RNA −)rna NN cord-001257-t21l6i3f 10 62 . . . cord-001257-t21l6i3f 11 1 We -PRON- PRP cord-001257-t21l6i3f 11 2 show show VBP cord-001257-t21l6i3f 11 3 that that IN cord-001257-t21l6i3f 11 4 the the DT cord-001257-t21l6i3f 11 5 binding binding NN cord-001257-t21l6i3f 11 6 of of IN cord-001257-t21l6i3f 11 7 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 11 8 and and CC cord-001257-t21l6i3f 11 9 AtRH5 atrh5 NN cord-001257-t21l6i3f 11 10 helicases helicase NNS cord-001257-t21l6i3f 11 11 to to IN cord-001257-t21l6i3f 11 12 the the DT cord-001257-t21l6i3f 11 13 TBSV TBSV NNP cord-001257-t21l6i3f 11 14 ( ( -LRB- cord-001257-t21l6i3f 11 15 −)RNA −)rna NN cord-001257-t21l6i3f 11 16 could could MD cord-001257-t21l6i3f 11 17 unwind unwind VB cord-001257-t21l6i3f 11 18 the the DT cord-001257-t21l6i3f 11 19 dsRNA dsrna NN cord-001257-t21l6i3f 11 20 structure structure NN cord-001257-t21l6i3f 11 21 within within IN cord-001257-t21l6i3f 11 22 the the DT cord-001257-t21l6i3f 11 23 RIII(− RIII(− NNPS cord-001257-t21l6i3f 11 24 ) ) -RRB- cord-001257-t21l6i3f 11 25 REN REN NNP cord-001257-t21l6i3f 11 26 . . . cord-001257-t21l6i3f 12 1 This this DT cord-001257-t21l6i3f 12 2 unique unique JJ cord-001257-t21l6i3f 12 3 characteristic characteristic NN cord-001257-t21l6i3f 12 4 allows allow VBZ cord-001257-t21l6i3f 12 5 the the DT cord-001257-t21l6i3f 12 6 eIF4AIII eIF4AIII -LRB- cord-001257-t21l6i3f 12 7 - - HYPH cord-001257-t21l6i3f 12 8 like like JJ cord-001257-t21l6i3f 12 9 helicases helicase NNS cord-001257-t21l6i3f 12 10 to to TO cord-001257-t21l6i3f 12 11 perform perform VB cord-001257-t21l6i3f 12 12 novel novel JJ cord-001257-t21l6i3f 12 13 pro pro JJ cord-001257-t21l6i3f 12 14 - - JJ cord-001257-t21l6i3f 12 15 viral viral JJ cord-001257-t21l6i3f 12 16 functions function NNS cord-001257-t21l6i3f 12 17 involving involve VBG cord-001257-t21l6i3f 12 18 the the DT cord-001257-t21l6i3f 12 19 RIII(− RIII(− NNPS cord-001257-t21l6i3f 12 20 ) ) -RRB- cord-001257-t21l6i3f 12 21 REN REN NNP cord-001257-t21l6i3f 12 22 in in IN cord-001257-t21l6i3f 12 23 stimulation stimulation NN cord-001257-t21l6i3f 12 24 of of IN cord-001257-t21l6i3f 12 25 plus plus JJ cord-001257-t21l6i3f 12 26 - - HYPH cord-001257-t21l6i3f 12 27 strand strand NN cord-001257-t21l6i3f 12 28 ( ( -LRB- cord-001257-t21l6i3f 12 29 + + CC cord-001257-t21l6i3f 12 30 ) ) -RRB- cord-001257-t21l6i3f 12 31 RNA rna NN cord-001257-t21l6i3f 12 32 synthesis synthesis NN cord-001257-t21l6i3f 12 33 . . . cord-001257-t21l6i3f 13 1 We -PRON- PRP cord-001257-t21l6i3f 13 2 also also RB cord-001257-t21l6i3f 13 3 show show VBP cord-001257-t21l6i3f 13 4 that that IN cord-001257-t21l6i3f 13 5 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 13 6 and and CC cord-001257-t21l6i3f 13 7 AtRH5 atrh5 NN cord-001257-t21l6i3f 13 8 helicases helicase NNS cord-001257-t21l6i3f 13 9 are be VBP cord-001257-t21l6i3f 13 10 components component NNS cord-001257-t21l6i3f 13 11 of of IN cord-001257-t21l6i3f 13 12 the the DT cord-001257-t21l6i3f 13 13 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 13 14 VRCs VRCs NNP cord-001257-t21l6i3f 13 15 based base VBN cord-001257-t21l6i3f 13 16 on on IN cord-001257-t21l6i3f 13 17 co co JJ cord-001257-t21l6i3f 13 18 - - JJ cord-001257-t21l6i3f 13 19 purification purification JJ cord-001257-t21l6i3f 13 20 experiments experiment NNS cord-001257-t21l6i3f 13 21 . . . cord-001257-t21l6i3f 14 1 We -PRON- PRP cord-001257-t21l6i3f 14 2 propose propose VBP cord-001257-t21l6i3f 14 3 that that IN cord-001257-t21l6i3f 14 4 eIF4AIII eIF4AIII NNS cord-001257-t21l6i3f 14 5 - - HYPH cord-001257-t21l6i3f 14 6 like like JJ cord-001257-t21l6i3f 14 7 helicases helicase NNS cord-001257-t21l6i3f 14 8 destabilize destabilize VBP cord-001257-t21l6i3f 14 9 dsRNA dsrna NN cord-001257-t21l6i3f 14 10 replication replication NN cord-001257-t21l6i3f 14 11 intermediate intermediate JJ cord-001257-t21l6i3f 14 12 within within IN cord-001257-t21l6i3f 14 13 the the DT cord-001257-t21l6i3f 14 14 RIII(− RIII(− NNPS cord-001257-t21l6i3f 14 15 ) ) -RRB- cord-001257-t21l6i3f 14 16 REN REN NNP cord-001257-t21l6i3f 14 17 that that WDT cord-001257-t21l6i3f 14 18 promotes promote VBZ cord-001257-t21l6i3f 14 19 bringing bring VBG cord-001257-t21l6i3f 14 20 the the DT cord-001257-t21l6i3f 14 21 5′ 5′ CD cord-001257-t21l6i3f 14 22 and and CC cord-001257-t21l6i3f 14 23 3′ 3′ CD cord-001257-t21l6i3f 14 24 terminal terminal NN cord-001257-t21l6i3f 14 25 ( ( -LRB- cord-001257-t21l6i3f 14 26 −)RNA −)rna NN cord-001257-t21l6i3f 14 27 sequences sequence NNS cord-001257-t21l6i3f 14 28 in in IN cord-001257-t21l6i3f 14 29 close close JJ cord-001257-t21l6i3f 14 30 vicinity vicinity NN cord-001257-t21l6i3f 14 31 via via IN cord-001257-t21l6i3f 14 32 long long JJ cord-001257-t21l6i3f 14 33 - - HYPH cord-001257-t21l6i3f 14 34 range range NN cord-001257-t21l6i3f 14 35 RNA RNA NNP cord-001257-t21l6i3f 14 36 - - HYPH cord-001257-t21l6i3f 14 37 RNA RNA NNP cord-001257-t21l6i3f 14 38 base base NN cord-001257-t21l6i3f 14 39 pairing pairing NN cord-001257-t21l6i3f 14 40 . . . cord-001257-t21l6i3f 15 1 This this DT cord-001257-t21l6i3f 15 2 newly newly RB cord-001257-t21l6i3f 15 3 formed form VBN cord-001257-t21l6i3f 15 4 RNA RNA NNP cord-001257-t21l6i3f 15 5 structure structure NN cord-001257-t21l6i3f 15 6 promoted promote VBN cord-001257-t21l6i3f 15 7 by by IN cord-001257-t21l6i3f 15 8 eIF4AIII eIF4AIII NNS cord-001257-t21l6i3f 15 9 helicase helicase VBN cord-001257-t21l6i3f 15 10 together together RB cord-001257-t21l6i3f 15 11 with with IN cord-001257-t21l6i3f 15 12 AtRH20 AtRH20 NNP cord-001257-t21l6i3f 15 13 helicase helicase NNP cord-001257-t21l6i3f 15 14 might may MD cord-001257-t21l6i3f 15 15 facilitate facilitate VB cord-001257-t21l6i3f 15 16 the the DT cord-001257-t21l6i3f 15 17 recycling recycling NN cord-001257-t21l6i3f 15 18 of of IN cord-001257-t21l6i3f 15 19 the the DT cord-001257-t21l6i3f 15 20 viral viral JJ cord-001257-t21l6i3f 15 21 replicases replicase NNS cord-001257-t21l6i3f 15 22 for for IN cord-001257-t21l6i3f 15 23 multiple multiple JJ cord-001257-t21l6i3f 15 24 rounds round NNS cord-001257-t21l6i3f 15 25 of of IN cord-001257-t21l6i3f 15 26 ( ( -LRB- cord-001257-t21l6i3f 15 27 + + NNS cord-001257-t21l6i3f 15 28 ) ) -RRB- cord-001257-t21l6i3f 15 29 -strand -strand CD cord-001257-t21l6i3f 15 30 synthesis synthesis NN cord-001257-t21l6i3f 15 31 , , , cord-001257-t21l6i3f 15 32 thus thus RB cord-001257-t21l6i3f 15 33 resulting result VBG cord-001257-t21l6i3f 15 34 in in IN cord-001257-t21l6i3f 15 35 asymmetrical asymmetrical JJ cord-001257-t21l6i3f 15 36 viral viral JJ cord-001257-t21l6i3f 15 37 replication replication NN cord-001257-t21l6i3f 15 38 . . . cord-001257-t21l6i3f 16 1 Host host NN cord-001257-t21l6i3f 16 2 factors factor NNS cord-001257-t21l6i3f 16 3 co co VBD cord-001257-t21l6i3f 16 4 - - VBD cord-001257-t21l6i3f 16 5 opted opt VBN cord-001257-t21l6i3f 16 6 for for IN cord-001257-t21l6i3f 16 7 replication replication NN cord-001257-t21l6i3f 16 8 of of IN cord-001257-t21l6i3f 16 9 plus plus NN cord-001257-t21l6i3f 16 10 - - HYPH cord-001257-t21l6i3f 16 11 stranded stranded JJ cord-001257-t21l6i3f 16 12 ( ( -LRB- cord-001257-t21l6i3f 16 13 + + CC cord-001257-t21l6i3f 16 14 ) ) -RRB- cord-001257-t21l6i3f 16 15 RNA RNA NNP cord-001257-t21l6i3f 16 16 viruses virus NNS cord-001257-t21l6i3f 16 17 are be VBP cord-001257-t21l6i3f 16 18 critical critical JJ cord-001257-t21l6i3f 16 19 in in IN cord-001257-t21l6i3f 16 20 each each DT cord-001257-t21l6i3f 16 21 step step NN cord-001257-t21l6i3f 16 22 of of IN cord-001257-t21l6i3f 16 23 the the DT cord-001257-t21l6i3f 16 24 well well RB cord-001257-t21l6i3f 16 25 - - HYPH cord-001257-t21l6i3f 16 26 orchestrated orchestrate VBN cord-001257-t21l6i3f 16 27 infection infection NN cord-001257-t21l6i3f 16 28 process process NN cord-001257-t21l6i3f 16 29 . . . cord-001257-t21l6i3f 17 1 After after IN cord-001257-t21l6i3f 17 2 translation translation NN cord-001257-t21l6i3f 17 3 of of IN cord-001257-t21l6i3f 17 4 the the DT cord-001257-t21l6i3f 17 5 viral viral JJ cord-001257-t21l6i3f 17 6 mRNA mrna NN cord-001257-t21l6i3f 17 7 - - HYPH cord-001257-t21l6i3f 17 8 sense sense NN cord-001257-t21l6i3f 17 9 genomic genomic JJ cord-001257-t21l6i3f 17 10 RNA(s RNA(s NNP cord-001257-t21l6i3f 17 11 ) ) -RRB- cord-001257-t21l6i3f 17 12 , , , cord-001257-t21l6i3f 17 13 the the DT cord-001257-t21l6i3f 17 14 viral viral NN cord-001257-t21l6i3f 17 15 ( ( -LRB- cord-001257-t21l6i3f 17 16 + + CD cord-001257-t21l6i3f 17 17 ) ) -RRB- cord-001257-t21l6i3f 17 18 RNA RNA NNP cord-001257-t21l6i3f 17 19 and and CC cord-001257-t21l6i3f 17 20 the the DT cord-001257-t21l6i3f 17 21 viral viral JJ cord-001257-t21l6i3f 17 22 replication replication NN cord-001257-t21l6i3f 17 23 proteins protein NNS cord-001257-t21l6i3f 17 24 together together RB cord-001257-t21l6i3f 17 25 with with IN cord-001257-t21l6i3f 17 26 host host NN cord-001257-t21l6i3f 17 27 RNA rna NN cord-001257-t21l6i3f 17 28 - - HYPH cord-001257-t21l6i3f 17 29 binding bind VBG cord-001257-t21l6i3f 17 30 proteins protein NNS cord-001257-t21l6i3f 17 31 ( ( -LRB- cord-001257-t21l6i3f 17 32 RBPs RBPs NNP cord-001257-t21l6i3f 17 33 ) ) -RRB- cord-001257-t21l6i3f 17 34 are be VBP cord-001257-t21l6i3f 17 35 recruited recruit VBN cord-001257-t21l6i3f 17 36 to to IN cord-001257-t21l6i3f 17 37 the the DT cord-001257-t21l6i3f 17 38 site site NN cord-001257-t21l6i3f 17 39 of of IN cord-001257-t21l6i3f 17 40 viral viral JJ cord-001257-t21l6i3f 17 41 replication replication NN cord-001257-t21l6i3f 17 42 in in IN cord-001257-t21l6i3f 17 43 membranous membranous JJ cord-001257-t21l6i3f 17 44 cellular cellular JJ cord-001257-t21l6i3f 17 45 compartments compartment NNS cord-001257-t21l6i3f 17 46 . . . cord-001257-t21l6i3f 18 1 Ultimately ultimately RB cord-001257-t21l6i3f 18 2 , , , cord-001257-t21l6i3f 18 3 the the DT cord-001257-t21l6i3f 18 4 process process NN cord-001257-t21l6i3f 18 5 leads lead VBZ cord-001257-t21l6i3f 18 6 to to IN cord-001257-t21l6i3f 18 7 the the DT cord-001257-t21l6i3f 18 8 assembly assembly NN cord-001257-t21l6i3f 18 9 of of IN cord-001257-t21l6i3f 18 10 the the DT cord-001257-t21l6i3f 18 11 membranebound membranebound NNP cord-001257-t21l6i3f 18 12 viral viral JJ cord-001257-t21l6i3f 18 13 replicase replicase NNP cord-001257-t21l6i3f 18 14 complexes complexes NNP cord-001257-t21l6i3f 18 15 ( ( -LRB- cord-001257-t21l6i3f 18 16 VRCs VRCs NNP cord-001257-t21l6i3f 18 17 ) ) -RRB- cord-001257-t21l6i3f 18 18 , , , cord-001257-t21l6i3f 18 19 followed follow VBN cord-001257-t21l6i3f 18 20 by by IN cord-001257-t21l6i3f 18 21 the the DT cord-001257-t21l6i3f 18 22 activation activation NN cord-001257-t21l6i3f 18 23 of of IN cord-001257-t21l6i3f 18 24 the the DT cord-001257-t21l6i3f 18 25 polymerase polymerase NN cord-001257-t21l6i3f 18 26 function function NN cord-001257-t21l6i3f 18 27 of of IN cord-001257-t21l6i3f 18 28 the the DT cord-001257-t21l6i3f 18 29 viral viral JJ cord-001257-t21l6i3f 18 30 RNA RNA NNP cord-001257-t21l6i3f 18 31 - - HYPH cord-001257-t21l6i3f 18 32 dependent dependent JJ cord-001257-t21l6i3f 18 33 RNA RNA NNP cord-001257-t21l6i3f 18 34 polymerase polymerase NN cord-001257-t21l6i3f 18 35 ( ( -LRB- cord-001257-t21l6i3f 18 36 RdRp RdRp NNP cord-001257-t21l6i3f 18 37 ) ) -RRB- cord-001257-t21l6i3f 18 38 , , , cord-001257-t21l6i3f 18 39 and and CC cord-001257-t21l6i3f 18 40 initiation initiation NN cord-001257-t21l6i3f 18 41 of of IN cord-001257-t21l6i3f 18 42 complementary complementary JJ cord-001257-t21l6i3f 18 43 RNA RNA NNP cord-001257-t21l6i3f 18 44 synthesis synthesis NN cord-001257-t21l6i3f 18 45 on on IN cord-001257-t21l6i3f 18 46 the the DT cord-001257-t21l6i3f 18 47 viral viral NN cord-001257-t21l6i3f 18 48 ( ( -LRB- cord-001257-t21l6i3f 18 49 + + CD cord-001257-t21l6i3f 18 50 ) ) -RRB- cord-001257-t21l6i3f 18 51 RNA rna NN cord-001257-t21l6i3f 18 52 template template NN cord-001257-t21l6i3f 18 53 [ [ -LRB- cord-001257-t21l6i3f 18 54 1 1 CD cord-001257-t21l6i3f 18 55 ] ] -RRB- cord-001257-t21l6i3f 18 56 [ [ -LRB- cord-001257-t21l6i3f 18 57 2 2 CD cord-001257-t21l6i3f 18 58 ] ] -RRB- cord-001257-t21l6i3f 18 59 [ [ -LRB- cord-001257-t21l6i3f 18 60 3 3 CD cord-001257-t21l6i3f 18 61 ] ] -RRB- cord-001257-t21l6i3f 18 62 [ [ -LRB- cord-001257-t21l6i3f 18 63 4 4 CD cord-001257-t21l6i3f 18 64 ] ] -RRB- cord-001257-t21l6i3f 18 65 . . . cord-001257-t21l6i3f 19 1 Subsequent subsequent JJ cord-001257-t21l6i3f 19 2 ( ( -LRB- cord-001257-t21l6i3f 19 3 + + CC cord-001257-t21l6i3f 20 1 ) ) -RRB- cord-001257-t21l6i3f 20 2 strand strand NN cord-001257-t21l6i3f 20 3 synthesis synthesis NN cord-001257-t21l6i3f 20 4 in in IN cord-001257-t21l6i3f 20 5 the the DT cord-001257-t21l6i3f 20 6 VRCs VRCs NNP cord-001257-t21l6i3f 20 7 takes take VBZ cord-001257-t21l6i3f 20 8 place place NN cord-001257-t21l6i3f 20 9 in in IN cord-001257-t21l6i3f 20 10 an an DT cord-001257-t21l6i3f 20 11 asymmetric asymmetric JJ cord-001257-t21l6i3f 20 12 manner manner NN cord-001257-t21l6i3f 20 13 , , , cord-001257-t21l6i3f 20 14 producing produce VBG cord-001257-t21l6i3f 20 15 excess excess JJ cord-001257-t21l6i3f 20 16 amounts amount NNS cord-001257-t21l6i3f 20 17 of of IN cord-001257-t21l6i3f 20 18 ( ( -LRB- cord-001257-t21l6i3f 20 19 + + NNS cord-001257-t21l6i3f 20 20 ) ) -RRB- cord-001257-t21l6i3f 21 1 -strand -strand CD cord-001257-t21l6i3f 21 2 progeny progeny NNS cord-001257-t21l6i3f 21 3 , , , cord-001257-t21l6i3f 21 4 which which WDT cord-001257-t21l6i3f 21 5 is be VBZ cord-001257-t21l6i3f 21 6 released release VBN cord-001257-t21l6i3f 21 7 from from IN cord-001257-t21l6i3f 21 8 replication replication NN cord-001257-t21l6i3f 21 9 to to TO cord-001257-t21l6i3f 21 10 participate participate VB cord-001257-t21l6i3f 21 11 in in IN cord-001257-t21l6i3f 21 12 encapsidation encapsidation NN cord-001257-t21l6i3f 21 13 , , , cord-001257-t21l6i3f 21 14 cell cell NN cord-001257-t21l6i3f 21 15 - - HYPH cord-001257-t21l6i3f 21 16 tocell tocell JJ cord-001257-t21l6i3f 21 17 movement movement NN cord-001257-t21l6i3f 21 18 and and CC cord-001257-t21l6i3f 21 19 other other JJ cord-001257-t21l6i3f 21 20 viral viral JJ cord-001257-t21l6i3f 21 21 processes process NNS cord-001257-t21l6i3f 21 22 . . . cord-001257-t21l6i3f 22 1 Although although IN cord-001257-t21l6i3f 22 2 the the DT cord-001257-t21l6i3f 22 3 roles role NNS cord-001257-t21l6i3f 22 4 of of IN cord-001257-t21l6i3f 22 5 host host NN cord-001257-t21l6i3f 22 6 factors factor NNS cord-001257-t21l6i3f 22 7 in in IN cord-001257-t21l6i3f 22 8 facilitating facilitate VBG cord-001257-t21l6i3f 22 9 the the DT cord-001257-t21l6i3f 22 10 replication replication NN cord-001257-t21l6i3f 22 11 process process NN cord-001257-t21l6i3f 22 12 of of IN cord-001257-t21l6i3f 22 13 ( ( -LRB- cord-001257-t21l6i3f 22 14 + + CC cord-001257-t21l6i3f 22 15 ) ) -RRB- cord-001257-t21l6i3f 22 16 RNA rna NN cord-001257-t21l6i3f 22 17 viruses virus NNS cord-001257-t21l6i3f 22 18 have have VBP cord-001257-t21l6i3f 22 19 been be VBN cord-001257-t21l6i3f 22 20 extensively extensively RB cord-001257-t21l6i3f 22 21 characterized characterize VBN cord-001257-t21l6i3f 22 22 in in IN cord-001257-t21l6i3f 22 23 recent recent JJ cord-001257-t21l6i3f 22 24 years year NNS cord-001257-t21l6i3f 22 25 [ [ -LRB- cord-001257-t21l6i3f 22 26 1 1 CD cord-001257-t21l6i3f 22 27 ] ] -RRB- cord-001257-t21l6i3f 22 28 [ [ -LRB- cord-001257-t21l6i3f 22 29 2 2 CD cord-001257-t21l6i3f 22 30 ] ] -RRB- cord-001257-t21l6i3f 22 31 [ [ -LRB- cord-001257-t21l6i3f 22 32 3 3 CD cord-001257-t21l6i3f 22 33 ] ] -RRB- cord-001257-t21l6i3f 23 1 [ [ -LRB- cord-001257-t21l6i3f 23 2 5 5 CD cord-001257-t21l6i3f 23 3 ] ] -RRB- cord-001257-t21l6i3f 23 4 [ [ -LRB- cord-001257-t21l6i3f 23 5 6 6 CD cord-001257-t21l6i3f 23 6 ] ] -RRB- cord-001257-t21l6i3f 23 7 [ [ -LRB- cord-001257-t21l6i3f 23 8 7 7 CD cord-001257-t21l6i3f 23 9 ] ] -RRB- cord-001257-t21l6i3f 24 1 [ [ -LRB- cord-001257-t21l6i3f 24 2 8 8 CD cord-001257-t21l6i3f 24 3 ] ] -RRB- cord-001257-t21l6i3f 24 4 [ [ -LRB- cord-001257-t21l6i3f 24 5 9 9 CD cord-001257-t21l6i3f 24 6 ] ] -RRB- cord-001257-t21l6i3f 24 7 [ [ -LRB- cord-001257-t21l6i3f 24 8 10 10 CD cord-001257-t21l6i3f 24 9 ] ] -RRB- cord-001257-t21l6i3f 24 10 [ [ -LRB- cord-001257-t21l6i3f 24 11 11 11 CD cord-001257-t21l6i3f 24 12 ] ] -RRB- cord-001257-t21l6i3f 24 13 , , , cord-001257-t21l6i3f 24 14 our -PRON- PRP$ cord-001257-t21l6i3f 24 15 current current JJ cord-001257-t21l6i3f 24 16 understanding understanding NN cord-001257-t21l6i3f 24 17 of of IN cord-001257-t21l6i3f 24 18 the the DT cord-001257-t21l6i3f 24 19 role role NN cord-001257-t21l6i3f 24 20 of of IN cord-001257-t21l6i3f 24 21 cellular cellular JJ cord-001257-t21l6i3f 24 22 RBPs rbp NNS cord-001257-t21l6i3f 24 23 , , , cord-001257-t21l6i3f 24 24 which which WDT cord-001257-t21l6i3f 24 25 constitute constitute VBP cord-001257-t21l6i3f 24 26 one one CD cord-001257-t21l6i3f 24 27 of of IN cord-001257-t21l6i3f 24 28 the the DT cord-001257-t21l6i3f 24 29 largest large JJS cord-001257-t21l6i3f 24 30 groups group NNS cord-001257-t21l6i3f 24 31 of of IN cord-001257-t21l6i3f 24 32 host host NN cord-001257-t21l6i3f 24 33 factors factor NNS cord-001257-t21l6i3f 24 34 identified identify VBN cord-001257-t21l6i3f 24 35 is be VBZ cord-001257-t21l6i3f 24 36 incomplete incomplete JJ cord-001257-t21l6i3f 24 37 [ [ -LRB- cord-001257-t21l6i3f 24 38 1 1 CD cord-001257-t21l6i3f 24 39 , , , cord-001257-t21l6i3f 24 40 12 12 CD cord-001257-t21l6i3f 24 41 , , , cord-001257-t21l6i3f 24 42 13 13 CD cord-001257-t21l6i3f 24 43 ] ] -RRB- cord-001257-t21l6i3f 24 44 . . . cord-001257-t21l6i3f 25 1 The the DT cord-001257-t21l6i3f 25 2 co co NN cord-001257-t21l6i3f 25 3 - - JJ cord-001257-t21l6i3f 25 4 opted opted JJ cord-001257-t21l6i3f 25 5 RBPs rbps NN cord-001257-t21l6i3f 25 6 likely likely RB cord-001257-t21l6i3f 25 7 affect affect VBP cord-001257-t21l6i3f 25 8 several several JJ cord-001257-t21l6i3f 25 9 steps step NNS cord-001257-t21l6i3f 25 10 in in IN cord-001257-t21l6i3f 25 11 viral viral JJ cord-001257-t21l6i3f 25 12 RNA RNA NNP cord-001257-t21l6i3f 25 13 replication replication NN cord-001257-t21l6i3f 25 14 , , , cord-001257-t21l6i3f 25 15 including include VBG cord-001257-t21l6i3f 25 16 viral viral JJ cord-001257-t21l6i3f 25 17 ( ( -LRB- cord-001257-t21l6i3f 25 18 + + CC cord-001257-t21l6i3f 25 19 ) ) -RRB- cord-001257-t21l6i3f 25 20 RNA rna NN cord-001257-t21l6i3f 25 21 recruitment recruitment NN cord-001257-t21l6i3f 25 22 , , , cord-001257-t21l6i3f 25 23 stabilization stabilization NN cord-001257-t21l6i3f 25 24 of of IN cord-001257-t21l6i3f 25 25 the the DT cord-001257-t21l6i3f 25 26 viral viral JJ cord-001257-t21l6i3f 25 27 RNA RNA NNP cord-001257-t21l6i3f 25 28 , , , cord-001257-t21l6i3f 25 29 VRC VRC NNP cord-001257-t21l6i3f 25 30 assembly assembly NN cord-001257-t21l6i3f 25 31 and and CC cord-001257-t21l6i3f 25 32 viral viral JJ cord-001257-t21l6i3f 25 33 RNA RNA NNP cord-001257-t21l6i3f 25 34 synthesis synthesis NN cord-001257-t21l6i3f 25 35 . . . cord-001257-t21l6i3f 26 1 Tomato tomato NN cord-001257-t21l6i3f 26 2 bushy bushy JJ cord-001257-t21l6i3f 26 3 stunt stunt NN cord-001257-t21l6i3f 26 4 virus virus NN cord-001257-t21l6i3f 26 5 ( ( -LRB- cord-001257-t21l6i3f 26 6 TBSV TBSV NNP cord-001257-t21l6i3f 26 7 ) ) -RRB- cord-001257-t21l6i3f 26 8 is be VBZ cord-001257-t21l6i3f 26 9 a a DT cord-001257-t21l6i3f 26 10 plant plant NN cord-001257-t21l6i3f 26 11 RNA RNA NNP cord-001257-t21l6i3f 26 12 virus virus NN cord-001257-t21l6i3f 26 13 with with IN cord-001257-t21l6i3f 26 14 a a DT cord-001257-t21l6i3f 26 15 single single JJ cord-001257-t21l6i3f 26 16 , , , cord-001257-t21l6i3f 26 17 4,800 4,800 CD cord-001257-t21l6i3f 26 18 nt nt RB cord-001257-t21l6i3f 26 19 genomic genomic JJ cord-001257-t21l6i3f 26 20 RNA RNA NNP cord-001257-t21l6i3f 26 21 and and CC cord-001257-t21l6i3f 26 22 has have VBZ cord-001257-t21l6i3f 26 23 two two CD cord-001257-t21l6i3f 26 24 essential essential JJ cord-001257-t21l6i3f 26 25 replication replication NN cord-001257-t21l6i3f 26 26 proteins protein NNS cord-001257-t21l6i3f 26 27 , , , cord-001257-t21l6i3f 26 28 p33 p33 NN cord-001257-t21l6i3f 26 29 and and CC cord-001257-t21l6i3f 26 30 p92 p92 NN cord-001257-t21l6i3f 26 31 pol pol NN cord-001257-t21l6i3f 26 32 , , , cord-001257-t21l6i3f 26 33 required require VBN cord-001257-t21l6i3f 26 34 for for IN cord-001257-t21l6i3f 26 35 TBSV TBSV NNP cord-001257-t21l6i3f 26 36 replicon replicon NNP cord-001257-t21l6i3f 27 1 ( ( -LRB- cord-001257-t21l6i3f 27 2 rep)RNA rep)RNA JJS cord-001257-t21l6i3f 27 3 replication replication NN cord-001257-t21l6i3f 27 4 in in IN cord-001257-t21l6i3f 27 5 yeast yeast NN cord-001257-t21l6i3f 27 6 ( ( -LRB- cord-001257-t21l6i3f 27 7 Saccharomyces Saccharomyces NNP cord-001257-t21l6i3f 27 8 cerevisiae cerevisiae NNS cord-001257-t21l6i3f 27 9 ) ) -RRB- cord-001257-t21l6i3f 27 10 model model NN cord-001257-t21l6i3f 27 11 host host NN cord-001257-t21l6i3f 27 12 [ [ -LRB- cord-001257-t21l6i3f 27 13 14 14 CD cord-001257-t21l6i3f 27 14 , , , cord-001257-t21l6i3f 27 15 15 15 CD cord-001257-t21l6i3f 27 16 ] ] -RRB- cord-001257-t21l6i3f 27 17 . . . cord-001257-t21l6i3f 28 1 The the DT cord-001257-t21l6i3f 28 2 membrane membrane NN cord-001257-t21l6i3f 28 3 - - HYPH cord-001257-t21l6i3f 28 4 bound bind VBN cord-001257-t21l6i3f 28 5 tombusvirus tombusvirus NN cord-001257-t21l6i3f 28 6 VRC VRC NNP cord-001257-t21l6i3f 28 7 contains contain VBZ cord-001257-t21l6i3f 28 8 p33 p33 NN cord-001257-t21l6i3f 28 9 and and CC cord-001257-t21l6i3f 28 10 p92 p92 NN cord-001257-t21l6i3f 28 11 pol pol NN cord-001257-t21l6i3f 28 12 , , , cord-001257-t21l6i3f 28 13 and and CC cord-001257-t21l6i3f 28 14 the the DT cord-001257-t21l6i3f 28 15 tombusviral tombusviral NN cord-001257-t21l6i3f 28 16 ( ( -LRB- cord-001257-t21l6i3f 28 17 + + NNS cord-001257-t21l6i3f 28 18 ) ) -RRB- cord-001257-t21l6i3f 28 19 repRNA repRNA NNS cord-001257-t21l6i3f 28 20 , , , cord-001257-t21l6i3f 28 21 which which WDT cord-001257-t21l6i3f 28 22 serves serve VBZ cord-001257-t21l6i3f 28 23 both both DT cord-001257-t21l6i3f 28 24 as as IN cord-001257-t21l6i3f 28 25 a a DT cord-001257-t21l6i3f 28 26 template template NN cord-001257-t21l6i3f 28 27 and and CC cord-001257-t21l6i3f 28 28 as as IN cord-001257-t21l6i3f 28 29 a a DT cord-001257-t21l6i3f 28 30 platform platform NN cord-001257-t21l6i3f 28 31 during during IN cord-001257-t21l6i3f 28 32 VRC VRC NNP cord-001257-t21l6i3f 28 33 assembly assembly NN cord-001257-t21l6i3f 28 34 and and CC cord-001257-t21l6i3f 28 35 activation activation NN cord-001257-t21l6i3f 28 36 [ [ -LRB- cord-001257-t21l6i3f 28 37 16 16 CD cord-001257-t21l6i3f 28 38 ] ] -RRB- cord-001257-t21l6i3f 28 39 [ [ -LRB- cord-001257-t21l6i3f 28 40 17 17 CD cord-001257-t21l6i3f 28 41 ] ] -RRB- cord-001257-t21l6i3f 28 42 [ [ -LRB- cord-001257-t21l6i3f 28 43 18 18 CD cord-001257-t21l6i3f 28 44 ] ] -RRB- cord-001257-t21l6i3f 28 45 [ [ -LRB- cord-001257-t21l6i3f 28 46 19 19 CD cord-001257-t21l6i3f 28 47 ] ] -RRB- cord-001257-t21l6i3f 28 48 [ [ -LRB- cord-001257-t21l6i3f 28 49 20 20 CD cord-001257-t21l6i3f 28 50 ] ] -RRB- cord-001257-t21l6i3f 28 51 . . . cord-001257-t21l6i3f 29 1 Interestingly interestingly RB cord-001257-t21l6i3f 29 2 , , , cord-001257-t21l6i3f 29 3 the the DT cord-001257-t21l6i3f 29 4 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 29 5 VRC VRC NNP cord-001257-t21l6i3f 29 6 contains contain VBZ cord-001257-t21l6i3f 29 7 at at IN cord-001257-t21l6i3f 29 8 least least RBS cord-001257-t21l6i3f 29 9 seven seven CD cord-001257-t21l6i3f 29 10 host host NN cord-001257-t21l6i3f 29 11 proteins protein NNS cord-001257-t21l6i3f 29 12 as as IN cord-001257-t21l6i3f 29 13 resident resident JJ cord-001257-t21l6i3f 29 14 members member NNS cord-001257-t21l6i3f 29 15 , , , cord-001257-t21l6i3f 29 16 including include VBG cord-001257-t21l6i3f 29 17 glyceraldehyde-3-phosphate glyceraldehyde-3-phosphate JJ cord-001257-t21l6i3f 29 18 dehydrogenase dehydrogenase NN cord-001257-t21l6i3f 29 19 ( ( -LRB- cord-001257-t21l6i3f 29 20 GAPDH GAPDH NNP cord-001257-t21l6i3f 29 21 , , , cord-001257-t21l6i3f 29 22 encoded encode VBN cord-001257-t21l6i3f 29 23 by by IN cord-001257-t21l6i3f 29 24 TDH2 TDH2 NNP cord-001257-t21l6i3f 29 25 and and CC cord-001257-t21l6i3f 29 26 TDH3 TDH3 NNP cord-001257-t21l6i3f 29 27 in in IN cord-001257-t21l6i3f 29 28 yeast yeast NN cord-001257-t21l6i3f 29 29 ) ) -RRB- cord-001257-t21l6i3f 29 30 [ [ -LRB- cord-001257-t21l6i3f 29 31 21 21 CD cord-001257-t21l6i3f 29 32 ] ] -RRB- cord-001257-t21l6i3f 29 33 , , , cord-001257-t21l6i3f 29 34 the the DT cord-001257-t21l6i3f 29 35 heat heat NN cord-001257-t21l6i3f 29 36 shock shock NN cord-001257-t21l6i3f 29 37 protein protein NN cord-001257-t21l6i3f 29 38 70 70 CD cord-001257-t21l6i3f 29 39 chaperones chaperone NNS cord-001257-t21l6i3f 29 40 ( ( -LRB- cord-001257-t21l6i3f 29 41 Hsp70 Hsp70 NNP cord-001257-t21l6i3f 29 42 , , , cord-001257-t21l6i3f 29 43 Ssa1/2p Ssa1/2p NNP cord-001257-t21l6i3f 29 44 in in IN cord-001257-t21l6i3f 29 45 yeast yeast NN cord-001257-t21l6i3f 29 46 ) ) -RRB- cord-001257-t21l6i3f 29 47 [ [ -LRB- cord-001257-t21l6i3f 29 48 22 22 CD cord-001257-t21l6i3f 29 49 ] ] -RRB- cord-001257-t21l6i3f 29 50 [ [ -LRB- cord-001257-t21l6i3f 29 51 23 23 CD cord-001257-t21l6i3f 29 52 ] ] -RRB- cord-001257-t21l6i3f 29 53 [ [ -LRB- cord-001257-t21l6i3f 29 54 24 24 CD cord-001257-t21l6i3f 29 55 ] ] -RRB- cord-001257-t21l6i3f 29 56 [ [ -LRB- cord-001257-t21l6i3f 29 57 25 25 CD cord-001257-t21l6i3f 29 58 ] ] -RRB- cord-001257-t21l6i3f 29 59 , , , cord-001257-t21l6i3f 29 60 pyruvate pyruvate NN cord-001257-t21l6i3f 29 61 decarboxylase decarboxylase NN cord-001257-t21l6i3f 29 62 ( ( -LRB- cord-001257-t21l6i3f 29 63 Pdc1p pdc1p NN cord-001257-t21l6i3f 29 64 ) ) -RRB- cord-001257-t21l6i3f 29 65 [ [ -LRB- cord-001257-t21l6i3f 29 66 25 25 CD cord-001257-t21l6i3f 29 67 ] ] -RRB- cord-001257-t21l6i3f 29 68 , , , cord-001257-t21l6i3f 29 69 Cdc34p Cdc34p NNP cord-001257-t21l6i3f 29 70 E2 E2 NNP cord-001257-t21l6i3f 29 71 ubiquitin ubiquitin NN cord-001257-t21l6i3f 29 72 conjugating conjugate VBG cord-001257-t21l6i3f 29 73 enzyme enzyme NN cord-001257-t21l6i3f 29 74 [ [ -LRB- cord-001257-t21l6i3f 29 75 26 26 CD cord-001257-t21l6i3f 29 76 ] ] -RRB- cord-001257-t21l6i3f 29 77 , , , cord-001257-t21l6i3f 29 78 eukaryotic eukaryotic JJ cord-001257-t21l6i3f 29 79 translation translation NN cord-001257-t21l6i3f 29 80 elongation elongation NN cord-001257-t21l6i3f 29 81 factor factor NN cord-001257-t21l6i3f 29 82 1A 1a NN cord-001257-t21l6i3f 29 83 ( ( -LRB- cord-001257-t21l6i3f 29 84 eEF1A eef1a CD cord-001257-t21l6i3f 29 85 ) ) -RRB- cord-001257-t21l6i3f 29 86 [ [ -LRB- cord-001257-t21l6i3f 29 87 27 27 CD cord-001257-t21l6i3f 29 88 , , , cord-001257-t21l6i3f 29 89 28 28 CD cord-001257-t21l6i3f 29 90 ] ] -RRB- cord-001257-t21l6i3f 29 91 , , , cord-001257-t21l6i3f 29 92 eEF1Bc eef1bc CD cord-001257-t21l6i3f 29 93 [ [ -LRB- cord-001257-t21l6i3f 29 94 29 29 CD cord-001257-t21l6i3f 29 95 ] ] -RRB- cord-001257-t21l6i3f 29 96 , , , cord-001257-t21l6i3f 29 97 and and CC cord-001257-t21l6i3f 29 98 Ded1p ded1p IN cord-001257-t21l6i3f 29 99 DEAD dead JJ cord-001257-t21l6i3f 29 100 - - HYPH cord-001257-t21l6i3f 29 101 box box NN cord-001257-t21l6i3f 29 102 helicase helicase NN cord-001257-t21l6i3f 29 103 [ [ -LRB- cord-001257-t21l6i3f 29 104 30 30 CD cord-001257-t21l6i3f 29 105 ] ] -RRB- cord-001257-t21l6i3f 29 106 . . . cord-001257-t21l6i3f 30 1 The the DT cord-001257-t21l6i3f 30 2 VRC VRC NNP cord-001257-t21l6i3f 30 3 also also RB cord-001257-t21l6i3f 30 4 contains contain VBZ cord-001257-t21l6i3f 30 5 two two CD cord-001257-t21l6i3f 30 6 temporary temporary JJ cord-001257-t21l6i3f 30 7 resident resident JJ cord-001257-t21l6i3f 30 8 proteins protein NNS cord-001257-t21l6i3f 30 9 , , , cord-001257-t21l6i3f 30 10 Pex19p Pex19p NNP cord-001257-t21l6i3f 30 11 shuttle shuttle NN cord-001257-t21l6i3f 30 12 protein protein NN cord-001257-t21l6i3f 30 13 [ [ -LRB- cord-001257-t21l6i3f 30 14 31 31 CD cord-001257-t21l6i3f 30 15 ] ] -RRB- cord-001257-t21l6i3f 30 16 and and CC cord-001257-t21l6i3f 30 17 the the DT cord-001257-t21l6i3f 30 18 Vps23p Vps23p NNP cord-001257-t21l6i3f 30 19 adaptor adaptor NN cord-001257-t21l6i3f 30 20 ESCRT ESCRT NNP cord-001257-t21l6i3f 30 21 protein protein NN cord-001257-t21l6i3f 30 22 [ [ -LRB- cord-001257-t21l6i3f 30 23 28 28 CD cord-001257-t21l6i3f 30 24 , , , cord-001257-t21l6i3f 30 25 32 32 CD cord-001257-t21l6i3f 30 26 , , , cord-001257-t21l6i3f 30 27 33 33 CD cord-001257-t21l6i3f 30 28 ] ] -RRB- cord-001257-t21l6i3f 30 29 . . . cord-001257-t21l6i3f 31 1 Detailed detailed JJ cord-001257-t21l6i3f 31 2 mechanistic mechanistic JJ cord-001257-t21l6i3f 31 3 studies study NNS cord-001257-t21l6i3f 31 4 revealed reveal VBD cord-001257-t21l6i3f 31 5 that that IN cord-001257-t21l6i3f 31 6 the the DT cord-001257-t21l6i3f 31 7 cellular cellular JJ cord-001257-t21l6i3f 31 8 Hsp70 Hsp70 NNP cord-001257-t21l6i3f 31 9 , , , cord-001257-t21l6i3f 31 10 eEF1A eef1a NN cord-001257-t21l6i3f 31 11 and and CC cord-001257-t21l6i3f 31 12 Vps23p Vps23p NNP cord-001257-t21l6i3f 31 13 are be VBP cord-001257-t21l6i3f 31 14 involved involve VBN cord-001257-t21l6i3f 31 15 in in IN cord-001257-t21l6i3f 31 16 the the DT cord-001257-t21l6i3f 31 17 assembly assembly NN cord-001257-t21l6i3f 31 18 of of IN cord-001257-t21l6i3f 31 19 the the DT cord-001257-t21l6i3f 31 20 VRC VRC NNP cord-001257-t21l6i3f 31 21 , , , cord-001257-t21l6i3f 31 22 while while IN cord-001257-t21l6i3f 31 23 the the DT cord-001257-t21l6i3f 31 24 functions function NNS cord-001257-t21l6i3f 31 25 of of IN cord-001257-t21l6i3f 31 26 host host NN cord-001257-t21l6i3f 31 27 RBPs rbp NNS cord-001257-t21l6i3f 31 28 , , , cord-001257-t21l6i3f 31 29 such such JJ cord-001257-t21l6i3f 31 30 as as IN cord-001257-t21l6i3f 31 31 eEF1A eef1a CD cord-001257-t21l6i3f 31 32 , , , cord-001257-t21l6i3f 31 33 eEF1Bc eEF1Bc NNP cord-001257-t21l6i3f 31 34 , , , cord-001257-t21l6i3f 31 35 GAPDH GAPDH NNP cord-001257-t21l6i3f 31 36 and and CC cord-001257-t21l6i3f 31 37 Ded1p Ded1p NNS cord-001257-t21l6i3f 31 38 , , , cord-001257-t21l6i3f 31 39 are be VBP cord-001257-t21l6i3f 31 40 to to TO cord-001257-t21l6i3f 31 41 regulate regulate VB cord-001257-t21l6i3f 31 42 viral viral JJ cord-001257-t21l6i3f 31 43 RNA RNA NNP cord-001257-t21l6i3f 31 44 synthesis synthesis NN cord-001257-t21l6i3f 31 45 by by IN cord-001257-t21l6i3f 31 46 the the DT cord-001257-t21l6i3f 31 47 VRC VRC NNP cord-001257-t21l6i3f 31 48 [ [ -LRB- cord-001257-t21l6i3f 31 49 3 3 CD cord-001257-t21l6i3f 31 50 , , , cord-001257-t21l6i3f 31 51 [ [ -LRB- cord-001257-t21l6i3f 31 52 21 21 CD cord-001257-t21l6i3f 31 53 ] ] -RRB- cord-001257-t21l6i3f 31 54 [ [ -LRB- cord-001257-t21l6i3f 31 55 22 22 CD cord-001257-t21l6i3f 31 56 ] ] -RRB- cord-001257-t21l6i3f 31 57 [ [ -LRB- cord-001257-t21l6i3f 31 58 23 23 CD cord-001257-t21l6i3f 31 59 ] ] -RRB- cord-001257-t21l6i3f 31 60 [ [ -LRB- cord-001257-t21l6i3f 31 61 24 24 CD cord-001257-t21l6i3f 31 62 ] ] -RRB- cord-001257-t21l6i3f 31 63 29 29 CD cord-001257-t21l6i3f 31 64 , , , cord-001257-t21l6i3f 31 65 30 30 CD cord-001257-t21l6i3f 31 66 , , , cord-001257-t21l6i3f 31 67 34 34 CD cord-001257-t21l6i3f 31 68 ] ] -RRB- cord-001257-t21l6i3f 31 69 . . . cord-001257-t21l6i3f 32 1 In in IN cord-001257-t21l6i3f 32 2 spite spite NN cord-001257-t21l6i3f 32 3 of of IN cord-001257-t21l6i3f 32 4 our -PRON- PRP$ cord-001257-t21l6i3f 32 5 growing grow VBG cord-001257-t21l6i3f 32 6 understanding understanding NN cord-001257-t21l6i3f 32 7 of of IN cord-001257-t21l6i3f 32 8 TBSV TBSV NNP cord-001257-t21l6i3f 32 9 replication replication NN cord-001257-t21l6i3f 32 10 and and CC cord-001257-t21l6i3f 32 11 TBSV TBSV NNP cord-001257-t21l6i3f 32 12 - - HYPH cord-001257-t21l6i3f 32 13 host host NN cord-001257-t21l6i3f 32 14 interaction interaction NN cord-001257-t21l6i3f 32 15 , , , cord-001257-t21l6i3f 32 16 many many JJ cord-001257-t21l6i3f 32 17 questions question NNS cord-001257-t21l6i3f 32 18 remain remain VBP cord-001257-t21l6i3f 32 19 . . . cord-001257-t21l6i3f 33 1 Indeed indeed RB cord-001257-t21l6i3f 33 2 , , , cord-001257-t21l6i3f 33 3 multiple multiple JJ cord-001257-t21l6i3f 33 4 genome genome NN cord-001257-t21l6i3f 33 5 - - HYPH cord-001257-t21l6i3f 33 6 wide wide JJ cord-001257-t21l6i3f 33 7 screens screen NNS cord-001257-t21l6i3f 33 8 and and CC cord-001257-t21l6i3f 33 9 global global JJ cord-001257-t21l6i3f 33 10 proteomics proteomic NNS cord-001257-t21l6i3f 33 11 approaches approach NNS cord-001257-t21l6i3f 33 12 with with IN cord-001257-t21l6i3f 33 13 TBSV TBSV NNP cord-001257-t21l6i3f 33 14 using use VBG cord-001257-t21l6i3f 33 15 yeast yeast NN cord-001257-t21l6i3f 33 16 as as IN cord-001257-t21l6i3f 33 17 host host NN cord-001257-t21l6i3f 33 18 identified identify VBN cord-001257-t21l6i3f 33 19 , , , cord-001257-t21l6i3f 33 20 500 500 CD cord-001257-t21l6i3f 33 21 host host NN cord-001257-t21l6i3f 33 22 factors factor NNS cord-001257-t21l6i3f 33 23 , , , cord-001257-t21l6i3f 33 24 which which WDT cord-001257-t21l6i3f 33 25 interact interact VBP cord-001257-t21l6i3f 33 26 with with IN cord-001257-t21l6i3f 33 27 viral viral JJ cord-001257-t21l6i3f 33 28 replication replication NN cord-001257-t21l6i3f 33 29 proteins protein NNS cord-001257-t21l6i3f 33 30 or or CC cord-001257-t21l6i3f 33 31 affect affect VB cord-001257-t21l6i3f 33 32 TBSV TBSV NNP cord-001257-t21l6i3f 33 33 replication replication NN cord-001257-t21l6i3f 33 34 [ [ -LRB- cord-001257-t21l6i3f 33 35 25 25 CD cord-001257-t21l6i3f 33 36 , , , cord-001257-t21l6i3f 33 37 26 26 CD cord-001257-t21l6i3f 33 38 , , , cord-001257-t21l6i3f 33 39 28 28 CD cord-001257-t21l6i3f 33 40 , , , cord-001257-t21l6i3f 33 41 [ [ -LRB- cord-001257-t21l6i3f 33 42 34 34 CD cord-001257-t21l6i3f 33 43 ] ] -RRB- cord-001257-t21l6i3f 33 44 [ [ -LRB- cord-001257-t21l6i3f 33 45 35 35 CD cord-001257-t21l6i3f 33 46 ] ] -RRB- cord-001257-t21l6i3f 33 47 [ [ -LRB- cord-001257-t21l6i3f 33 48 36 36 CD cord-001257-t21l6i3f 33 49 ] ] -RRB- cord-001257-t21l6i3f 33 50 [ [ -LRB- cord-001257-t21l6i3f 33 51 37 37 CD cord-001257-t21l6i3f 33 52 ] ] -RRB- cord-001257-t21l6i3f 33 53 [ [ -LRB- cord-001257-t21l6i3f 33 54 38 38 CD cord-001257-t21l6i3f 33 55 ] ] -RRB- cord-001257-t21l6i3f 33 56 . . . cord-001257-t21l6i3f 34 1 Among among IN cord-001257-t21l6i3f 34 2 the the DT cord-001257-t21l6i3f 34 3 host host NN cord-001257-t21l6i3f 34 4 proteins protein NNS cord-001257-t21l6i3f 34 5 identified identify VBN cord-001257-t21l6i3f 34 6 are be VBP cord-001257-t21l6i3f 34 7 11 11 CD cord-001257-t21l6i3f 34 8 host host NN cord-001257-t21l6i3f 34 9 ATP ATP NNP cord-001257-t21l6i3f 34 10 - - HYPH cord-001257-t21l6i3f 34 11 dependent dependent JJ cord-001257-t21l6i3f 34 12 RNA RNA NNP cord-001257-t21l6i3f 34 13 helicases helicase NNS cord-001257-t21l6i3f 34 14 out out IN cord-001257-t21l6i3f 34 15 of of IN cord-001257-t21l6i3f 34 16 39 39 CD cord-001257-t21l6i3f 34 17 known know VBN cord-001257-t21l6i3f 34 18 yeast yeast NN cord-001257-t21l6i3f 34 19 helicases helicase NNS cord-001257-t21l6i3f 34 20 that that WDT cord-001257-t21l6i3f 34 21 could could MD cord-001257-t21l6i3f 34 22 be be VB cord-001257-t21l6i3f 34 23 involved involve VBN cord-001257-t21l6i3f 34 24 in in IN cord-001257-t21l6i3f 34 25 TBSV TBSV NNP cord-001257-t21l6i3f 34 26 replication replication NN cord-001257-t21l6i3f 34 27 . . . cord-001257-t21l6i3f 35 1 DEAD DEAD NNP cord-001257-t21l6i3f 35 2 - - HYPH cord-001257-t21l6i3f 35 3 box box NN cord-001257-t21l6i3f 35 4 proteins protein NNS cord-001257-t21l6i3f 35 5 constitute constitute VBP cord-001257-t21l6i3f 35 6 the the DT cord-001257-t21l6i3f 35 7 largest large JJS cord-001257-t21l6i3f 35 8 family family NN cord-001257-t21l6i3f 35 9 of of IN cord-001257-t21l6i3f 35 10 RNA RNA NNP cord-001257-t21l6i3f 35 11 helicases helicase NNS cord-001257-t21l6i3f 35 12 , , , cord-001257-t21l6i3f 35 13 which which WDT cord-001257-t21l6i3f 35 14 perform perform VBP cord-001257-t21l6i3f 35 15 ATP ATP NNP cord-001257-t21l6i3f 35 16 - - HYPH cord-001257-t21l6i3f 35 17 dependent dependent JJ cord-001257-t21l6i3f 35 18 RNA RNA NNP cord-001257-t21l6i3f 35 19 duplex duplex NN cord-001257-t21l6i3f 35 20 unwinding unwinding NN cord-001257-t21l6i3f 35 21 , , , cord-001257-t21l6i3f 35 22 RNA RNA NNP cord-001257-t21l6i3f 35 23 folding folding NN cord-001257-t21l6i3f 35 24 , , , cord-001257-t21l6i3f 35 25 remodeling remodeling NN cord-001257-t21l6i3f 35 26 of of IN cord-001257-t21l6i3f 35 27 RNA RNA NNP cord-001257-t21l6i3f 35 28 - - HYPH cord-001257-t21l6i3f 35 29 protein protein NN cord-001257-t21l6i3f 35 30 complexes complex NNS cord-001257-t21l6i3f 35 31 , , , cord-001257-t21l6i3f 35 32 and and CC cord-001257-t21l6i3f 35 33 RNA RNA NNP cord-001257-t21l6i3f 35 34 clamping clamp VBG cord-001257-t21l6i3f 35 35 in in IN cord-001257-t21l6i3f 35 36 cells cell NNS cord-001257-t21l6i3f 35 37 . . . cord-001257-t21l6i3f 36 1 DEAD DEAD NNP cord-001257-t21l6i3f 36 2 - - HYPH cord-001257-t21l6i3f 36 3 box box NN cord-001257-t21l6i3f 36 4 helicases helicase NNS cord-001257-t21l6i3f 36 5 are be VBP cord-001257-t21l6i3f 36 6 involved involve VBN cord-001257-t21l6i3f 36 7 in in IN cord-001257-t21l6i3f 36 8 all all DT cord-001257-t21l6i3f 36 9 aspects aspect NNS cord-001257-t21l6i3f 36 10 of of IN cord-001257-t21l6i3f 36 11 cellular cellular JJ cord-001257-t21l6i3f 36 12 metabolism metabolism NN cord-001257-t21l6i3f 36 13 [ [ -LRB- cord-001257-t21l6i3f 36 14 39 39 CD cord-001257-t21l6i3f 36 15 ] ] -RRB- cord-001257-t21l6i3f 36 16 [ [ -LRB- cord-001257-t21l6i3f 36 17 40 40 CD cord-001257-t21l6i3f 36 18 ] ] -RRB- cord-001257-t21l6i3f 36 19 [ [ -LRB- cord-001257-t21l6i3f 36 20 41 41 CD cord-001257-t21l6i3f 36 21 ] ] -RRB- cord-001257-t21l6i3f 36 22 and and CC cord-001257-t21l6i3f 36 23 affect affect VBP cord-001257-t21l6i3f 36 24 replication replication NN cord-001257-t21l6i3f 36 25 of of IN cord-001257-t21l6i3f 36 26 many many JJ cord-001257-t21l6i3f 36 27 viruses virus NNS cord-001257-t21l6i3f 36 28 [ [ -LRB- cord-001257-t21l6i3f 36 29 42 42 CD cord-001257-t21l6i3f 36 30 ] ] -RRB- cord-001257-t21l6i3f 36 31 , , , cord-001257-t21l6i3f 36 32 including include VBG cord-001257-t21l6i3f 36 33 plant plant NN cord-001257-t21l6i3f 36 34 RNA RNA NNP cord-001257-t21l6i3f 36 35 viruses virus NNS cord-001257-t21l6i3f 36 36 [ [ -LRB- cord-001257-t21l6i3f 36 37 43 43 CD cord-001257-t21l6i3f 36 38 ] ] -RRB- cord-001257-t21l6i3f 36 39 . . . cord-001257-t21l6i3f 37 1 Plant Plant NNP cord-001257-t21l6i3f 37 2 RNA RNA NNP cord-001257-t21l6i3f 37 3 helicases helicase NNS cord-001257-t21l6i3f 37 4 are be VBP cord-001257-t21l6i3f 37 5 also also RB cord-001257-t21l6i3f 37 6 implicated implicate VBN cord-001257-t21l6i3f 37 7 in in IN cord-001257-t21l6i3f 37 8 plant plant NN cord-001257-t21l6i3f 37 9 responses response NNS cord-001257-t21l6i3f 37 10 to to IN cord-001257-t21l6i3f 37 11 abiotic abiotic JJ cord-001257-t21l6i3f 37 12 stress stress NN cord-001257-t21l6i3f 37 13 and and CC cord-001257-t21l6i3f 37 14 pathogen pathogen NN cord-001257-t21l6i3f 37 15 infections infection NNS cord-001257-t21l6i3f 37 16 [ [ -LRB- cord-001257-t21l6i3f 37 17 44 44 CD cord-001257-t21l6i3f 37 18 ] ] -RRB- cord-001257-t21l6i3f 37 19 [ [ -LRB- cord-001257-t21l6i3f 37 20 45 45 CD cord-001257-t21l6i3f 37 21 ] ] -RRB- cord-001257-t21l6i3f 37 22 [ [ -LRB- cord-001257-t21l6i3f 37 23 46 46 CD cord-001257-t21l6i3f 37 24 ] ] -RRB- cord-001257-t21l6i3f 37 25 . . . cord-001257-t21l6i3f 38 1 The the DT cord-001257-t21l6i3f 38 2 many many JJ cord-001257-t21l6i3f 38 3 cellular cellular JJ cord-001257-t21l6i3f 38 4 RNA RNA NNP cord-001257-t21l6i3f 38 5 helicases helicase NNS cord-001257-t21l6i3f 38 6 identified identify VBN cord-001257-t21l6i3f 38 7 in in IN cord-001257-t21l6i3f 38 8 TBSV TBSV NNP cord-001257-t21l6i3f 38 9 screens screen NNS cord-001257-t21l6i3f 38 10 are be VBP cord-001257-t21l6i3f 38 11 intriguing intriguing JJ cord-001257-t21l6i3f 38 12 because because IN cord-001257-t21l6i3f 38 13 , , , cord-001257-t21l6i3f 38 14 tombusviruses tombusviruse NNS cord-001257-t21l6i3f 38 15 and and CC cord-001257-t21l6i3f 38 16 other other JJ cord-001257-t21l6i3f 38 17 small small JJ cord-001257-t21l6i3f 38 18 RNA RNA NNP cord-001257-t21l6i3f 38 19 viruses virus NNS cord-001257-t21l6i3f 38 20 do do VBP cord-001257-t21l6i3f 38 21 not not RB cord-001257-t21l6i3f 38 22 code code VB cord-001257-t21l6i3f 38 23 for for IN cord-001257-t21l6i3f 38 24 their -PRON- PRP$ cord-001257-t21l6i3f 38 25 own own JJ cord-001257-t21l6i3f 38 26 helicases helicase NNS cord-001257-t21l6i3f 38 27 [ [ -LRB- cord-001257-t21l6i3f 38 28 47 47 CD cord-001257-t21l6i3f 38 29 , , , cord-001257-t21l6i3f 38 30 48 48 CD cord-001257-t21l6i3f 38 31 ] ] -RRB- cord-001257-t21l6i3f 38 32 . . . cord-001257-t21l6i3f 39 1 These these DT cord-001257-t21l6i3f 39 2 viruses virus NNS cord-001257-t21l6i3f 39 3 likely likely RB cord-001257-t21l6i3f 39 4 recruit recruit VBP cord-001257-t21l6i3f 39 5 host host NN cord-001257-t21l6i3f 39 6 helicases helicase NNS cord-001257-t21l6i3f 39 7 in in IN cord-001257-t21l6i3f 39 8 order order NN cord-001257-t21l6i3f 39 9 to to TO cord-001257-t21l6i3f 39 10 facilitate facilitate VB cord-001257-t21l6i3f 39 11 viral viral JJ cord-001257-t21l6i3f 39 12 replication replication NN cord-001257-t21l6i3f 39 13 . . . cord-001257-t21l6i3f 40 1 The the DT cord-001257-t21l6i3f 40 2 best well RBS cord-001257-t21l6i3f 40 3 - - HYPH cord-001257-t21l6i3f 40 4 characterized characterize VBN cord-001257-t21l6i3f 40 5 member member NN cord-001257-t21l6i3f 40 6 of of IN cord-001257-t21l6i3f 40 7 the the DT cord-001257-t21l6i3f 40 8 helicase helicase NNP cord-001257-t21l6i3f 40 9 family family NN cord-001257-t21l6i3f 40 10 involved involve VBN cord-001257-t21l6i3f 40 11 in in IN cord-001257-t21l6i3f 40 12 tombusvirus tombusvirus NN cord-001257-t21l6i3f 40 13 replication replication NN cord-001257-t21l6i3f 40 14 is be VBZ cord-001257-t21l6i3f 40 15 the the DT cord-001257-t21l6i3f 40 16 yeast yeast NN cord-001257-t21l6i3f 40 17 Ded1p ded1p IN cord-001257-t21l6i3f 40 18 ( ( -LRB- cord-001257-t21l6i3f 40 19 the the DT cord-001257-t21l6i3f 40 20 human human JJ cord-001257-t21l6i3f 40 21 DDX3like DDX3like NNP cord-001257-t21l6i3f 40 22 ) ) -RRB- cord-001257-t21l6i3f 40 23 and and CC cord-001257-t21l6i3f 40 24 the the DT cord-001257-t21l6i3f 40 25 similar similar JJ cord-001257-t21l6i3f 40 26 plant plant NN cord-001257-t21l6i3f 40 27 AtRH20 AtRH20 NNP cord-001257-t21l6i3f 40 28 DEAD DEAD NNP cord-001257-t21l6i3f 40 29 - - HYPH cord-001257-t21l6i3f 40 30 box box NN cord-001257-t21l6i3f 40 31 helicases helicase NNS cord-001257-t21l6i3f 40 32 , , , cord-001257-t21l6i3f 40 33 both both DT cord-001257-t21l6i3f 40 34 of of IN cord-001257-t21l6i3f 40 35 which which WDT cord-001257-t21l6i3f 40 36 promote promote VBP cord-001257-t21l6i3f 40 37 ( ( -LRB- cord-001257-t21l6i3f 40 38 + + NN cord-001257-t21l6i3f 40 39 ) ) -RRB- cord-001257-t21l6i3f 40 40 -strand -strand CD cord-001257-t21l6i3f 40 41 synthesis synthesis NN cord-001257-t21l6i3f 40 42 [ [ -LRB- cord-001257-t21l6i3f 40 43 30 30 CD cord-001257-t21l6i3f 40 44 ] ] -RRB- cord-001257-t21l6i3f 40 45 . . . cord-001257-t21l6i3f 41 1 Ded1p ded1p UH cord-001257-t21l6i3f 41 2 and and CC cord-001257-t21l6i3f 41 3 AtRH20 atrh20 TO cord-001257-t21l6i3f 41 4 bind bind VB cord-001257-t21l6i3f 41 5 to to IN cord-001257-t21l6i3f 41 6 the the DT cord-001257-t21l6i3f 41 7 39-end 39-end CD cord-001257-t21l6i3f 41 8 of of IN cord-001257-t21l6i3f 41 9 the the DT cord-001257-t21l6i3f 41 10 TBSV TBSV NNP cord-001257-t21l6i3f 41 11 minus minus JJ cord-001257-t21l6i3f 41 12 - - HYPH cord-001257-t21l6i3f 41 13 strand strand NN cord-001257-t21l6i3f 41 14 RNA RNA NNP cord-001257-t21l6i3f 41 15 , , , cord-001257-t21l6i3f 41 16 making make VBG cord-001257-t21l6i3f 41 17 the the DT cord-001257-t21l6i3f 41 18 promoter promoter NN cord-001257-t21l6i3f 41 19 sequence sequence NN cord-001257-t21l6i3f 41 20 accessible accessible JJ cord-001257-t21l6i3f 41 21 to to IN cord-001257-t21l6i3f 41 22 p92 p92 NN cord-001257-t21l6i3f 41 23 pol pol NN cord-001257-t21l6i3f 41 24 for for IN cord-001257-t21l6i3f 41 25 initiation initiation NN cord-001257-t21l6i3f 41 26 of of IN cord-001257-t21l6i3f 41 27 ( ( -LRB- cord-001257-t21l6i3f 41 28 + + CD cord-001257-t21l6i3f 41 29 ) ) -RRB- cord-001257-t21l6i3f 41 30 -strand -strand CD cord-001257-t21l6i3f 41 31 RNA RNA NNP cord-001257-t21l6i3f 41 32 synthesis synthesis NN cord-001257-t21l6i3f 41 33 . . . cord-001257-t21l6i3f 42 1 Additional additional JJ cord-001257-t21l6i3f 42 2 characterization characterization NN cord-001257-t21l6i3f 42 3 of of IN cord-001257-t21l6i3f 42 4 Ded1p ded1p NN cord-001257-t21l6i3f 42 5 and and CC cord-001257-t21l6i3f 42 6 the the DT cord-001257-t21l6i3f 42 7 similar similar JJ cord-001257-t21l6i3f 42 8 yeast yeast NN cord-001257-t21l6i3f 42 9 Dbp2p Dbp2p NNP cord-001257-t21l6i3f 42 10 ( ( -LRB- cord-001257-t21l6i3f 42 11 similar similar JJ cord-001257-t21l6i3f 42 12 to to IN cord-001257-t21l6i3f 42 13 human human JJ cord-001257-t21l6i3f 42 14 p68 p68 CD cord-001257-t21l6i3f 42 15 ) ) -RRB- cord-001257-t21l6i3f 42 16 DEAD DEAD NNP cord-001257-t21l6i3f 42 17 - - HYPH cord-001257-t21l6i3f 42 18 box box NN cord-001257-t21l6i3f 42 19 helicases helicase NNS cord-001257-t21l6i3f 42 20 revealed reveal VBD cord-001257-t21l6i3f 42 21 that that IN cord-001257-t21l6i3f 42 22 these these DT cord-001257-t21l6i3f 42 23 helicases helicase NNS cord-001257-t21l6i3f 42 24 play play VBP cord-001257-t21l6i3f 42 25 major major JJ cord-001257-t21l6i3f 42 26 and and CC cord-001257-t21l6i3f 42 27 overlapping overlapping JJ cord-001257-t21l6i3f 42 28 roles role NNS cord-001257-t21l6i3f 42 29 in in IN cord-001257-t21l6i3f 42 30 ( ( -LRB- cord-001257-t21l6i3f 42 31 + + CC cord-001257-t21l6i3f 43 1 ) ) -RRB- cord-001257-t21l6i3f 43 2 -strand -strand CD cord-001257-t21l6i3f 43 3 synthesis synthesis NN cord-001257-t21l6i3f 43 4 [ [ -LRB- cord-001257-t21l6i3f 43 5 30 30 CD cord-001257-t21l6i3f 43 6 , , , cord-001257-t21l6i3f 43 7 49 49 CD cord-001257-t21l6i3f 43 8 ] ] -RRB- cord-001257-t21l6i3f 43 9 . . . cord-001257-t21l6i3f 44 1 Altogether altogether RB cord-001257-t21l6i3f 44 2 , , , cord-001257-t21l6i3f 44 3 the the DT cord-001257-t21l6i3f 44 4 identification identification NN cord-001257-t21l6i3f 44 5 of of IN cord-001257-t21l6i3f 44 6 11 11 CD cord-001257-t21l6i3f 44 7 yeast yeast NN cord-001257-t21l6i3f 44 8 RNA RNA NNP cord-001257-t21l6i3f 44 9 helicases helicase NNS cord-001257-t21l6i3f 44 10 involved involve VBN cord-001257-t21l6i3f 44 11 in in IN cord-001257-t21l6i3f 44 12 tombusvirus tombusvirus NN cord-001257-t21l6i3f 44 13 replication replication NN cord-001257-t21l6i3f 44 14 suggests suggest VBZ cord-001257-t21l6i3f 44 15 that that IN cord-001257-t21l6i3f 44 16 tombusviruses tombusviruses NNP cord-001257-t21l6i3f 44 17 likely likely RB cord-001257-t21l6i3f 44 18 co co VB cord-001257-t21l6i3f 44 19 - - VB cord-001257-t21l6i3f 44 20 opt opt VB cord-001257-t21l6i3f 44 21 a a DT cord-001257-t21l6i3f 44 22 number number NN cord-001257-t21l6i3f 44 23 of of IN cord-001257-t21l6i3f 44 24 host host NN cord-001257-t21l6i3f 44 25 RNA RNA NNP cord-001257-t21l6i3f 44 26 helicases helicase NNS cord-001257-t21l6i3f 44 27 and and CC cord-001257-t21l6i3f 44 28 these these DT cord-001257-t21l6i3f 44 29 helicases helicase NNS cord-001257-t21l6i3f 44 30 might may MD cord-001257-t21l6i3f 44 31 have have VB cord-001257-t21l6i3f 44 32 a a DT cord-001257-t21l6i3f 44 33 number number NN cord-001257-t21l6i3f 44 34 of of IN cord-001257-t21l6i3f 44 35 unique unique JJ cord-001257-t21l6i3f 44 36 functions function NNS cord-001257-t21l6i3f 44 37 in in IN cord-001257-t21l6i3f 44 38 viral viral JJ cord-001257-t21l6i3f 44 39 replication replication NN cord-001257-t21l6i3f 44 40 . . . cord-001257-t21l6i3f 45 1 In in IN cord-001257-t21l6i3f 45 2 this this DT cord-001257-t21l6i3f 45 3 paper paper NN cord-001257-t21l6i3f 45 4 , , , cord-001257-t21l6i3f 45 5 we -PRON- PRP cord-001257-t21l6i3f 45 6 characterized characterize VBD cord-001257-t21l6i3f 45 7 the the DT cord-001257-t21l6i3f 45 8 novel novel JJ cord-001257-t21l6i3f 45 9 pro pro JJ cord-001257-t21l6i3f 45 10 - - JJ cord-001257-t21l6i3f 45 11 viral viral JJ cord-001257-t21l6i3f 45 12 functions function NNS cord-001257-t21l6i3f 45 13 of of IN cord-001257-t21l6i3f 45 14 two two CD cord-001257-t21l6i3f 45 15 yeast yeast NN cord-001257-t21l6i3f 45 16 RNA RNA NNP cord-001257-t21l6i3f 45 17 helicases helicase NNS cord-001257-t21l6i3f 45 18 , , , cord-001257-t21l6i3f 45 19 which which WDT cord-001257-t21l6i3f 45 20 were be VBD cord-001257-t21l6i3f 45 21 among among IN cord-001257-t21l6i3f 45 22 those those DT cord-001257-t21l6i3f 45 23 identified identify VBN cord-001257-t21l6i3f 45 24 in in IN cord-001257-t21l6i3f 45 25 previous previous JJ cord-001257-t21l6i3f 45 26 screens screen NNS cord-001257-t21l6i3f 45 27 , , , cord-001257-t21l6i3f 45 28 and and CC cord-001257-t21l6i3f 45 29 their -PRON- PRP$ cord-001257-t21l6i3f 45 30 plant plant NN cord-001257-t21l6i3f 45 31 orthologs ortholog NNS cord-001257-t21l6i3f 45 32 in in IN cord-001257-t21l6i3f 45 33 TBSV TBSV NNP cord-001257-t21l6i3f 45 34 replication replication NN cord-001257-t21l6i3f 45 35 . . . cord-001257-t21l6i3f 46 1 We -PRON- PRP cord-001257-t21l6i3f 46 2 found find VBD cord-001257-t21l6i3f 46 3 that that IN cord-001257-t21l6i3f 46 4 the the DT cord-001257-t21l6i3f 46 5 yeast yeast NN cord-001257-t21l6i3f 46 6 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 46 7 ( ( -LRB- cord-001257-t21l6i3f 46 8 DEAD DEAD NNP cord-001257-t21l6i3f 46 9 box box NN cord-001257-t21l6i3f 46 10 protein protein NN cord-001257-t21l6i3f 46 11 3 3 CD cord-001257-t21l6i3f 46 12 , , , cord-001257-t21l6i3f 46 13 human human JJ cord-001257-t21l6i3f 46 14 DDX5-like DDX5-like NNP cord-001257-t21l6i3f 46 15 ) ) -RRB- cord-001257-t21l6i3f 46 16 and and CC cord-001257-t21l6i3f 46 17 Fal1p fal1p XX cord-001257-t21l6i3f 46 18 ( ( -LRB- cord-001257-t21l6i3f 46 19 eukaryotic eukaryotic JJ cord-001257-t21l6i3f 46 20 translation translation NN cord-001257-t21l6i3f 46 21 initiation initiation NN cord-001257-t21l6i3f 46 22 factor factor NN cord-001257-t21l6i3f 46 23 4AIII 4aiii CD cord-001257-t21l6i3f 46 24 - - HYPH cord-001257-t21l6i3f 46 25 like like JJ cord-001257-t21l6i3f 46 26 ) ) -RRB- cord-001257-t21l6i3f 46 27 DEAD dead JJ cord-001257-t21l6i3f 46 28 box box NN cord-001257-t21l6i3f 46 29 helicases helicase NNS cord-001257-t21l6i3f 46 30 , , , cord-001257-t21l6i3f 46 31 which which WDT cord-001257-t21l6i3f 46 32 are be VBP cord-001257-t21l6i3f 46 33 involved involve VBN cord-001257-t21l6i3f 46 34 in in IN cord-001257-t21l6i3f 46 35 ribosome ribosome NN cord-001257-t21l6i3f 46 36 biogenesis biogenesis NN cord-001257-t21l6i3f 46 37 in in IN cord-001257-t21l6i3f 46 38 yeast yeast NN cord-001257-t21l6i3f 46 39 [ [ -LRB- cord-001257-t21l6i3f 46 40 50 50 CD cord-001257-t21l6i3f 46 41 ] ] -RRB- cord-001257-t21l6i3f 46 42 [ [ -LRB- cord-001257-t21l6i3f 46 43 51 51 CD cord-001257-t21l6i3f 46 44 ] ] -RRB- cord-001257-t21l6i3f 46 45 [ [ -LRB- cord-001257-t21l6i3f 46 46 52 52 CD cord-001257-t21l6i3f 46 47 ] ] -RRB- cord-001257-t21l6i3f 46 48 , , , cord-001257-t21l6i3f 46 49 and and CC cord-001257-t21l6i3f 46 50 the the DT cord-001257-t21l6i3f 46 51 orthologous orthologous NNP cord-001257-t21l6i3f 46 52 Arabidopsis Arabidopsis NNP cord-001257-t21l6i3f 46 53 RH2 RH2 NNP cord-001257-t21l6i3f 46 54 and and CC cord-001257-t21l6i3f 46 55 RH5 rh5 NN cord-001257-t21l6i3f 46 56 helicases helicase NNS cord-001257-t21l6i3f 46 57 bind bind VBP cord-001257-t21l6i3f 46 58 to to IN cord-001257-t21l6i3f 46 59 a a DT cord-001257-t21l6i3f 46 60 critical critical JJ cord-001257-t21l6i3f 46 61 replication replication NN cord-001257-t21l6i3f 46 62 enhancer enhancer NN cord-001257-t21l6i3f 46 63 element element NN cord-001257-t21l6i3f 46 64 ( ( -LRB- cord-001257-t21l6i3f 46 65 REN REN NNP cord-001257-t21l6i3f 46 66 ) ) -RRB- cord-001257-t21l6i3f 46 67 present present JJ cord-001257-t21l6i3f 46 68 in in IN cord-001257-t21l6i3f 46 69 a a DT cord-001257-t21l6i3f 46 70 59 59 CD cord-001257-t21l6i3f 46 71 proximal proximal JJ cord-001257-t21l6i3f 46 72 region region NN cord-001257-t21l6i3f 46 73 of of IN cord-001257-t21l6i3f 46 74 the the DT cord-001257-t21l6i3f 46 75 TBSV TBSV NNP cord-001257-t21l6i3f 46 76 minus minus JJ cord-001257-t21l6i3f 46 77 - - HYPH cord-001257-t21l6i3f 46 78 strand strand NN cord-001257-t21l6i3f 46 79 RNA RNA NNP cord-001257-t21l6i3f 46 80 . . . cord-001257-t21l6i3f 47 1 We -PRON- PRP cord-001257-t21l6i3f 47 2 show show VBP cord-001257-t21l6i3f 47 3 that that IN cord-001257-t21l6i3f 47 4 these these DT cord-001257-t21l6i3f 47 5 cellular cellular JJ cord-001257-t21l6i3f 47 6 helicases helicase NNS cord-001257-t21l6i3f 47 7 can can MD cord-001257-t21l6i3f 47 8 locally locally RB cord-001257-t21l6i3f 47 9 unwind unwind VB cord-001257-t21l6i3f 47 10 the the DT cord-001257-t21l6i3f 47 11 double double RB cord-001257-t21l6i3f 47 12 - - HYPH cord-001257-t21l6i3f 47 13 stranded stranded JJ cord-001257-t21l6i3f 47 14 ( ( -LRB- cord-001257-t21l6i3f 47 15 ds ds NNP cord-001257-t21l6i3f 47 16 ) ) -RRB- cord-001257-t21l6i3f 47 17 structure structure NN cord-001257-t21l6i3f 47 18 within within IN cord-001257-t21l6i3f 47 19 the the DT cord-001257-t21l6i3f 47 20 REN REN NNP cord-001257-t21l6i3f 47 21 of of IN cord-001257-t21l6i3f 47 22 the the DT cord-001257-t21l6i3f 47 23 replication replication NN cord-001257-t21l6i3f 47 24 intermediate intermediate JJ cord-001257-t21l6i3f 47 25 in in IN cord-001257-t21l6i3f 47 26 vitro vitro FW cord-001257-t21l6i3f 47 27 . . . cord-001257-t21l6i3f 48 1 These these DT cord-001257-t21l6i3f 48 2 activities activity NNS cord-001257-t21l6i3f 48 3 by by IN cord-001257-t21l6i3f 48 4 the the DT cord-001257-t21l6i3f 48 5 host host NN cord-001257-t21l6i3f 48 6 helicases helicase NNS cord-001257-t21l6i3f 48 7 enhance enhance VBP cord-001257-t21l6i3f 48 8 in in IN cord-001257-t21l6i3f 48 9 vitro vitro FW cord-001257-t21l6i3f 48 10 replication replication NN cord-001257-t21l6i3f 48 11 and and CC cord-001257-t21l6i3f 48 12 plus plus JJ cord-001257-t21l6i3f 48 13 - - HYPH cord-001257-t21l6i3f 48 14 strand strand NN cord-001257-t21l6i3f 48 15 RNA RNA NNP cord-001257-t21l6i3f 48 16 synthesis synthesis NN cord-001257-t21l6i3f 48 17 , , , cord-001257-t21l6i3f 48 18 and and CC cord-001257-t21l6i3f 48 19 the the DT cord-001257-t21l6i3f 48 20 accumulation accumulation NN cord-001257-t21l6i3f 48 21 of of IN cord-001257-t21l6i3f 48 22 TBSV TBSV NNP cord-001257-t21l6i3f 48 23 RNA RNA NNP cord-001257-t21l6i3f 48 24 in in IN cord-001257-t21l6i3f 48 25 yeast yeast NN cord-001257-t21l6i3f 48 26 and and CC cord-001257-t21l6i3f 48 27 plants plant NNS cord-001257-t21l6i3f 48 28 . . . cord-001257-t21l6i3f 49 1 We -PRON- PRP cord-001257-t21l6i3f 49 2 also also RB cord-001257-t21l6i3f 49 3 demonstrate demonstrate VBP cord-001257-t21l6i3f 49 4 that that IN cord-001257-t21l6i3f 49 5 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 49 6 and and CC cord-001257-t21l6i3f 49 7 AtRH5 atrh5 NN cord-001257-t21l6i3f 49 8 helicases helicase NNS cord-001257-t21l6i3f 49 9 work work VBP cord-001257-t21l6i3f 49 10 synergistically synergistically RB cord-001257-t21l6i3f 49 11 with with IN cord-001257-t21l6i3f 49 12 the the DT cord-001257-t21l6i3f 49 13 DDX3-like DDX3-like NNP cord-001257-t21l6i3f 49 14 AtRH20 AtRH20 NNP cord-001257-t21l6i3f 49 15 helicase helicase NNP cord-001257-t21l6i3f 49 16 ( ( -LRB- cord-001257-t21l6i3f 49 17 that that DT cord-001257-t21l6i3f 49 18 binds bind VBZ cord-001257-t21l6i3f 49 19 to to IN cord-001257-t21l6i3f 49 20 the the DT cord-001257-t21l6i3f 49 21 39 39 CD cord-001257-t21l6i3f 49 22 promoter promoter NN cord-001257-t21l6i3f 49 23 sequence sequence NN cord-001257-t21l6i3f 49 24 ) ) -RRB- cord-001257-t21l6i3f 50 1 to to TO cord-001257-t21l6i3f 50 2 facilitate facilitate VB cord-001257-t21l6i3f 50 3 plus plus JJ cord-001257-t21l6i3f 50 4 - - HYPH cord-001257-t21l6i3f 50 5 strand strand NN cord-001257-t21l6i3f 50 6 synthesis synthesis NN cord-001257-t21l6i3f 50 7 in in IN cord-001257-t21l6i3f 50 8 an an DT cord-001257-t21l6i3f 50 9 asymmetric asymmetric JJ cord-001257-t21l6i3f 50 10 manner manner NN cord-001257-t21l6i3f 50 11 . . . cord-001257-t21l6i3f 51 1 Altogether altogether RB cord-001257-t21l6i3f 51 2 , , , cord-001257-t21l6i3f 51 3 these these DT cord-001257-t21l6i3f 51 4 co co NN cord-001257-t21l6i3f 51 5 - - JJ cord-001257-t21l6i3f 51 6 opted opt VBN cord-001257-t21l6i3f 51 7 host host NN cord-001257-t21l6i3f 51 8 DEAD dead JJ cord-001257-t21l6i3f 51 9 box box NN cord-001257-t21l6i3f 51 10 helicases helicase NNS cord-001257-t21l6i3f 51 11 greatly greatly RB cord-001257-t21l6i3f 51 12 enhance enhance VB cord-001257-t21l6i3f 51 13 TBSV TBSV NNP cord-001257-t21l6i3f 51 14 replication replication NN cord-001257-t21l6i3f 51 15 by by IN cord-001257-t21l6i3f 51 16 interacting interact VBG cord-001257-t21l6i3f 51 17 with with IN cord-001257-t21l6i3f 51 18 the the DT cord-001257-t21l6i3f 51 19 viral viral NN cord-001257-t21l6i3f 51 20 ( ( -LRB- cord-001257-t21l6i3f 51 21 2)RNA 2)rna CD cord-001257-t21l6i3f 51 22 and and CC cord-001257-t21l6i3f 51 23 the the DT cord-001257-t21l6i3f 51 24 replication replication NN cord-001257-t21l6i3f 51 25 proteins protein NNS cord-001257-t21l6i3f 51 26 within within IN cord-001257-t21l6i3f 51 27 the the DT cord-001257-t21l6i3f 51 28 VRCs VRCs NNP cord-001257-t21l6i3f 51 29 . . . cord-001257-t21l6i3f 52 1 Overexpression overexpression NN cord-001257-t21l6i3f 52 2 of of IN cord-001257-t21l6i3f 52 3 yeast yeast NN cord-001257-t21l6i3f 52 4 eIF4AIII eIF4AIII NNS cord-001257-t21l6i3f 52 5 - - : cord-001257-t21l6i3f 52 6 like like IN cord-001257-t21l6i3f 52 7 Fal1p Fal1p NNP cord-001257-t21l6i3f 52 8 or or CC cord-001257-t21l6i3f 52 9 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 52 10 DEAD DEAD NNP cord-001257-t21l6i3f 52 11 - - HYPH cord-001257-t21l6i3f 52 12 box box NN cord-001257-t21l6i3f 52 13 RNA RNA NNP cord-001257-t21l6i3f 52 14 helicases helicase NNS cord-001257-t21l6i3f 52 15 and and CC cord-001257-t21l6i3f 52 16 the the DT cord-001257-t21l6i3f 52 17 orthologous orthologous JJ cord-001257-t21l6i3f 52 18 plant plant NN cord-001257-t21l6i3f 52 19 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 52 20 and and CC cord-001257-t21l6i3f 52 21 DDX5-like DDX5-like NNP cord-001257-t21l6i3f 53 1 AtRH5 atrh5 NN cord-001257-t21l6i3f 53 2 helicases helicase NNS cord-001257-t21l6i3f 53 3 enhances enhance VBZ cord-001257-t21l6i3f 53 4 TBSV TBSV NNP cord-001257-t21l6i3f 53 5 RNA RNA NNP cord-001257-t21l6i3f 53 6 replication replication NN cord-001257-t21l6i3f 53 7 in in IN cord-001257-t21l6i3f 53 8 yeast yeast NN cord-001257-t21l6i3f 53 9 and and CC cord-001257-t21l6i3f 53 10 plants plant NNS cord-001257-t21l6i3f 53 11 To to TO cord-001257-t21l6i3f 53 12 characterize characterize VB cord-001257-t21l6i3f 53 13 the the DT cord-001257-t21l6i3f 53 14 functions function NNS cord-001257-t21l6i3f 53 15 of of IN cord-001257-t21l6i3f 53 16 host host NN cord-001257-t21l6i3f 53 17 RNA RNA NNP cord-001257-t21l6i3f 53 18 helicases helicase NNS cord-001257-t21l6i3f 53 19 in in IN cord-001257-t21l6i3f 53 20 TBSV TBSV NNP cord-001257-t21l6i3f 53 21 replication replication NN cord-001257-t21l6i3f 53 22 , , , cord-001257-t21l6i3f 53 23 first first RB cord-001257-t21l6i3f 53 24 we -PRON- PRP cord-001257-t21l6i3f 53 25 overexpressed overexpresse VBD cord-001257-t21l6i3f 53 26 5 5 CD cord-001257-t21l6i3f 53 27 yeast yeast NN cord-001257-t21l6i3f 53 28 RNA RNA NNP cord-001257-t21l6i3f 53 29 helicases helicase NNS cord-001257-t21l6i3f 53 30 in in IN cord-001257-t21l6i3f 53 31 yeast yeast NN cord-001257-t21l6i3f 53 32 and and CC cord-001257-t21l6i3f 53 33 tested test VBD cord-001257-t21l6i3f 53 34 their -PRON- PRP$ cord-001257-t21l6i3f 53 35 effects effect NNS cord-001257-t21l6i3f 53 36 on on IN cord-001257-t21l6i3f 53 37 TBSV TBSV NNP cord-001257-t21l6i3f 53 38 replicon replicon NN cord-001257-t21l6i3f 54 1 ( ( -LRB- cord-001257-t21l6i3f 54 2 rep)RNA rep)RNA JJS cord-001257-t21l6i3f 54 3 accumulation accumulation NN cord-001257-t21l6i3f 54 4 via via IN cord-001257-t21l6i3f 54 5 Northern northern JJ cord-001257-t21l6i3f 54 6 blotting blotting NN cord-001257-t21l6i3f 54 7 ( ( -LRB- cord-001257-t21l6i3f 54 8 Fig Fig NNP cord-001257-t21l6i3f 54 9 . . . cord-001257-t21l6i3f 54 10 S1 S1 NNP cord-001257-t21l6i3f 54 11 ) ) -RRB- cord-001257-t21l6i3f 54 12 . . . cord-001257-t21l6i3f 55 1 These these DT cord-001257-t21l6i3f 55 2 helicases helicase NNS cord-001257-t21l6i3f 55 3 were be VBD cord-001257-t21l6i3f 55 4 chosen choose VBN cord-001257-t21l6i3f 55 5 from from IN cord-001257-t21l6i3f 55 6 the the DT cord-001257-t21l6i3f 55 7 11 11 CD cord-001257-t21l6i3f 55 8 previously previously RB cord-001257-t21l6i3f 55 9 identified identify VBN cord-001257-t21l6i3f 55 10 yeast yeast NN cord-001257-t21l6i3f 55 11 helicases helicase NNS cord-001257-t21l6i3f 55 12 from from IN cord-001257-t21l6i3f 55 13 several several JJ cord-001257-t21l6i3f 55 14 complementary complementary JJ cord-001257-t21l6i3f 55 15 high high JJ cord-001257-t21l6i3f 55 16 throughput throughput NN cord-001257-t21l6i3f 55 17 screens screen NNS cord-001257-t21l6i3f 55 18 using use VBG cord-001257-t21l6i3f 55 19 yeast yeast NN cord-001257-t21l6i3f 55 20 and and CC cord-001257-t21l6i3f 55 21 tombusviruses tombusviruse NNS cord-001257-t21l6i3f 55 22 [ [ -LRB- cord-001257-t21l6i3f 55 23 26 26 CD cord-001257-t21l6i3f 55 24 , , , cord-001257-t21l6i3f 55 25 28 28 CD cord-001257-t21l6i3f 55 26 , , , cord-001257-t21l6i3f 55 27 35 35 CD cord-001257-t21l6i3f 55 28 , , , cord-001257-t21l6i3f 55 29 36 36 CD cord-001257-t21l6i3f 55 30 , , , cord-001257-t21l6i3f 55 31 38 38 CD cord-001257-t21l6i3f 55 32 , , , cord-001257-t21l6i3f 55 33 53 53 CD cord-001257-t21l6i3f 55 34 , , , cord-001257-t21l6i3f 55 35 54 54 CD cord-001257-t21l6i3f 55 36 ] ] -RRB- cord-001257-t21l6i3f 55 37 . . . cord-001257-t21l6i3f 56 1 Overexpression overexpression NN cord-001257-t21l6i3f 56 2 of of IN cord-001257-t21l6i3f 56 3 all all DT cord-001257-t21l6i3f 56 4 5 5 CD cord-001257-t21l6i3f 56 5 host host NN cord-001257-t21l6i3f 56 6 RNA RNA NNP cord-001257-t21l6i3f 56 7 helicases helicase NNS cord-001257-t21l6i3f 56 8 increased increase VBD cord-001257-t21l6i3f 56 9 TBSV TBSV NNP cord-001257-t21l6i3f 56 10 accumulation accumulation NN cord-001257-t21l6i3f 56 11 , , , cord-001257-t21l6i3f 56 12 with with IN cord-001257-t21l6i3f 56 13 the the DT cord-001257-t21l6i3f 56 14 yeast yeast NN cord-001257-t21l6i3f 56 15 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 56 16 showing show VBG cord-001257-t21l6i3f 56 17 the the DT cord-001257-t21l6i3f 56 18 highest high JJS cord-001257-t21l6i3f 56 19 ( ( -LRB- cord-001257-t21l6i3f 56 20 over over IN cord-001257-t21l6i3f 56 21 2-fold 2-fold CD cord-001257-t21l6i3f 56 22 increase increase NN cord-001257-t21l6i3f 56 23 ) ) -RRB- cord-001257-t21l6i3f 56 24 stimulation stimulation NN cord-001257-t21l6i3f 56 25 ( ( -LRB- cord-001257-t21l6i3f 56 26 Fig fig NN cord-001257-t21l6i3f 56 27 . . . cord-001257-t21l6i3f 57 1 S1A s1a NN cord-001257-t21l6i3f 57 2 - - HYPH cord-001257-t21l6i3f 57 3 B B NNP cord-001257-t21l6i3f 57 4 ) ) -RRB- cord-001257-t21l6i3f 57 5 . . . cord-001257-t21l6i3f 58 1 The the DT cord-001257-t21l6i3f 58 2 overexpression overexpression NN cord-001257-t21l6i3f 58 3 of of IN cord-001257-t21l6i3f 58 4 these these DT cord-001257-t21l6i3f 58 5 host host NN cord-001257-t21l6i3f 58 6 helicases helicase NNS cord-001257-t21l6i3f 58 7 did do VBD cord-001257-t21l6i3f 58 8 not not RB cord-001257-t21l6i3f 58 9 affect affect VB cord-001257-t21l6i3f 58 10 the the DT cord-001257-t21l6i3f 58 11 accumulation accumulation NN cord-001257-t21l6i3f 58 12 of of IN cord-001257-t21l6i3f 58 13 p33 p33 CD cord-001257-t21l6i3f 58 14 replication replication NN cord-001257-t21l6i3f 58 15 protein protein NN cord-001257-t21l6i3f 58 16 ( ( -LRB- cord-001257-t21l6i3f 58 17 Fig Fig NNP cord-001257-t21l6i3f 58 18 . . . cord-001257-t21l6i3f 58 19 S1A s1a NN cord-001257-t21l6i3f 58 20 - - HYPH cord-001257-t21l6i3f 58 21 B B NNP cord-001257-t21l6i3f 58 22 ) ) -RRB- cord-001257-t21l6i3f 58 23 , , , cord-001257-t21l6i3f 58 24 suggesting suggest VBG cord-001257-t21l6i3f 58 25 that that IN cord-001257-t21l6i3f 58 26 the the DT cord-001257-t21l6i3f 58 27 effects effect NNS cord-001257-t21l6i3f 58 28 of of IN cord-001257-t21l6i3f 58 29 this this DT cord-001257-t21l6i3f 58 30 group group NN cord-001257-t21l6i3f 58 31 of of IN cord-001257-t21l6i3f 58 32 RNA RNA NNP cord-001257-t21l6i3f 58 33 helicases helicase NNS cord-001257-t21l6i3f 58 34 are be VBP cord-001257-t21l6i3f 58 35 not not RB cord-001257-t21l6i3f 58 36 through through IN cord-001257-t21l6i3f 58 37 increased increase VBN cord-001257-t21l6i3f 58 38 translation translation NN cord-001257-t21l6i3f 58 39 of of IN cord-001257-t21l6i3f 58 40 viral viral JJ cord-001257-t21l6i3f 58 41 replication replication NN cord-001257-t21l6i3f 58 42 proteins protein NNS cord-001257-t21l6i3f 58 43 . . . cord-001257-t21l6i3f 59 1 Altogether altogether RB cord-001257-t21l6i3f 59 2 , , , cord-001257-t21l6i3f 59 3 the the DT cord-001257-t21l6i3f 59 4 observed observed JJ cord-001257-t21l6i3f 59 5 30-to-140 30-to-140 CD cord-001257-t21l6i3f 59 6 % % NN cord-001257-t21l6i3f 59 7 increase increase NN cord-001257-t21l6i3f 59 8 in in IN cord-001257-t21l6i3f 59 9 TBSV TBSV NNP cord-001257-t21l6i3f 59 10 RNA RNA NNP cord-001257-t21l6i3f 59 11 accumulation accumulation NN cord-001257-t21l6i3f 59 12 due due IN cord-001257-t21l6i3f 59 13 to to IN cord-001257-t21l6i3f 59 14 overexpression overexpression NN cord-001257-t21l6i3f 59 15 of of IN cord-001257-t21l6i3f 59 16 these these DT cord-001257-t21l6i3f 59 17 yeast yeast NN cord-001257-t21l6i3f 59 18 helicases helicase NNS cord-001257-t21l6i3f 59 19 is be VBZ cord-001257-t21l6i3f 59 20 significant significant JJ cord-001257-t21l6i3f 59 21 since since IN cord-001257-t21l6i3f 59 22 overexpression overexpression NN cord-001257-t21l6i3f 59 23 of of IN cord-001257-t21l6i3f 59 24 most most JJS cord-001257-t21l6i3f 59 25 yeast yeast NN cord-001257-t21l6i3f 59 26 proteins protein NNS cord-001257-t21l6i3f 59 27 nonspecifically nonspecifically RB cord-001257-t21l6i3f 59 28 reduces reduce VBZ cord-001257-t21l6i3f 59 29 TBSV TBSV NNP cord-001257-t21l6i3f 59 30 accumulation accumulation NN cord-001257-t21l6i3f 59 31 by by IN cord-001257-t21l6i3f 59 32 20 20 CD cord-001257-t21l6i3f 59 33 - - SYM cord-001257-t21l6i3f 59 34 30 30 CD cord-001257-t21l6i3f 59 35 % % NN cord-001257-t21l6i3f 59 36 as as IN cord-001257-t21l6i3f 59 37 demonstrated demonstrate VBN cord-001257-t21l6i3f 59 38 before before IN cord-001257-t21l6i3f 59 39 based base VBN cord-001257-t21l6i3f 59 40 on on IN cord-001257-t21l6i3f 59 41 individual individual JJ cord-001257-t21l6i3f 59 42 overexpression overexpression NN cord-001257-t21l6i3f 59 43 of of IN cord-001257-t21l6i3f 59 44 5,500 5,500 CD cord-001257-t21l6i3f 59 45 yeast yeast NN cord-001257-t21l6i3f 59 46 proteins protein NNS cord-001257-t21l6i3f 59 47 [ [ -LRB- cord-001257-t21l6i3f 59 48 26 26 CD cord-001257-t21l6i3f 59 49 , , , cord-001257-t21l6i3f 59 50 54 54 CD cord-001257-t21l6i3f 59 51 ] ] -RRB- cord-001257-t21l6i3f 59 52 . . . cord-001257-t21l6i3f 60 1 Thus thus RB cord-001257-t21l6i3f 60 2 , , , cord-001257-t21l6i3f 60 3 these these DT cord-001257-t21l6i3f 60 4 helicases helicase NNS cord-001257-t21l6i3f 60 5 likely likely RB cord-001257-t21l6i3f 60 6 play play VB cord-001257-t21l6i3f 60 7 stimulatory stimulatory JJ cord-001257-t21l6i3f 60 8 roles role NNS cord-001257-t21l6i3f 60 9 in in IN cord-001257-t21l6i3f 60 10 TBSV TBSV NNP cord-001257-t21l6i3f 60 11 replication replication NN cord-001257-t21l6i3f 60 12 . . . cord-001257-t21l6i3f 61 1 For for IN cord-001257-t21l6i3f 61 2 additional additional JJ cord-001257-t21l6i3f 61 3 in in IN cord-001257-t21l6i3f 61 4 - - HYPH cord-001257-t21l6i3f 61 5 depth depth NN cord-001257-t21l6i3f 61 6 studies study NNS cord-001257-t21l6i3f 61 7 , , , cord-001257-t21l6i3f 61 8 we -PRON- PRP cord-001257-t21l6i3f 61 9 have have VBP cord-001257-t21l6i3f 61 10 selected select VBN cord-001257-t21l6i3f 61 11 the the DT cord-001257-t21l6i3f 61 12 yeast yeast NN cord-001257-t21l6i3f 61 13 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 61 14 and and CC cord-001257-t21l6i3f 61 15 the the DT cord-001257-t21l6i3f 61 16 highly highly RB cord-001257-t21l6i3f 61 17 similar similar JJ cord-001257-t21l6i3f 61 18 Fal1p fal1p XX cord-001257-t21l6i3f 61 19 ( ( -LRB- cord-001257-t21l6i3f 61 20 human human NN cord-001257-t21l6i3f 61 21 eIF4AIII eIF4AIII NNS cord-001257-t21l6i3f 61 22 - - HYPH cord-001257-t21l6i3f 61 23 like like JJ cord-001257-t21l6i3f 61 24 ) ) -RRB- cord-001257-t21l6i3f 61 25 together together RB cord-001257-t21l6i3f 61 26 with with IN cord-001257-t21l6i3f 61 27 the the DT cord-001257-t21l6i3f 61 28 orthologous orthologous NNP cord-001257-t21l6i3f 61 29 Arabidopsis Arabidopsis NNP cord-001257-t21l6i3f 61 30 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 61 31 ( ( -LRB- cord-001257-t21l6i3f 61 32 Fal1p fal1p IN cord-001257-t21l6i3f 61 33 ortholog ortholog NNP cord-001257-t21l6i3f 61 34 and and CC cord-001257-t21l6i3f 61 35 human human JJ cord-001257-t21l6i3f 61 36 eIF4AIII eIF4AIII NNS cord-001257-t21l6i3f 61 37 - - HYPH cord-001257-t21l6i3f 61 38 like like JJ cord-001257-t21l6i3f 61 39 ) ) -RRB- cord-001257-t21l6i3f 62 1 and and CC cord-001257-t21l6i3f 62 2 AtRH5 AtRH5 NNP cord-001257-t21l6i3f 62 3 ( ( -LRB- cord-001257-t21l6i3f 62 4 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 62 5 ortholog ortholog NNP cord-001257-t21l6i3f 62 6 , , , cord-001257-t21l6i3f 62 7 human human NNP cord-001257-t21l6i3f 62 8 DDX5-like DDX5-like NNP cord-001257-t21l6i3f 62 9 ) ) -RRB- cord-001257-t21l6i3f 62 10 helicases helicase NNS cord-001257-t21l6i3f 62 11 [ [ -LRB- cord-001257-t21l6i3f 62 12 44 44 CD cord-001257-t21l6i3f 62 13 ] ] -RRB- cord-001257-t21l6i3f 62 14 ] ] -RRB- cord-001257-t21l6i3f 62 15 . . . cord-001257-t21l6i3f 63 1 Overexpression Overexpression NNP cord-001257-t21l6i3f 63 2 of of IN cord-001257-t21l6i3f 63 3 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 63 4 and and CC cord-001257-t21l6i3f 63 5 AtRH5 atrh5 NN cord-001257-t21l6i3f 63 6 stimulated stimulate VBN cord-001257-t21l6i3f 63 7 ( ( -LRB- cord-001257-t21l6i3f 63 8 up up IN cord-001257-t21l6i3f 63 9 to to TO cord-001257-t21l6i3f 63 10 3-fold 3-fold CD cord-001257-t21l6i3f 63 11 increase increase NN cord-001257-t21l6i3f 63 12 ) ) -RRB- cord-001257-t21l6i3f 64 1 TBSV TBSV NNP cord-001257-t21l6i3f 64 2 repRNA repRNA NNP cord-001257-t21l6i3f 64 3 accumulation accumulation NN cord-001257-t21l6i3f 64 4 in in IN cord-001257-t21l6i3f 64 5 yeast yeast NN cord-001257-t21l6i3f 64 6 ( ( -LRB- cord-001257-t21l6i3f 64 7 Fig Fig NNP cord-001257-t21l6i3f 64 8 . . . cord-001257-t21l6i3f 64 9 1A 1a NN cord-001257-t21l6i3f 64 10 ) ) -RRB- cord-001257-t21l6i3f 64 11 . . . cord-001257-t21l6i3f 65 1 The the DT cord-001257-t21l6i3f 65 2 stimulation stimulation NN cord-001257-t21l6i3f 65 3 of of IN cord-001257-t21l6i3f 65 4 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 65 5 accumulation accumulation NN cord-001257-t21l6i3f 65 6 ( ( -LRB- cord-001257-t21l6i3f 65 7 we -PRON- PRP cord-001257-t21l6i3f 65 8 used use VBD cord-001257-t21l6i3f 65 9 Cucumber Cucumber NNP cord-001257-t21l6i3f 65 10 necrosis necrosis NN cord-001257-t21l6i3f 65 11 virus virus NN cord-001257-t21l6i3f 65 12 , , , cord-001257-t21l6i3f 65 13 CNV CNV NNP cord-001257-t21l6i3f 65 14 , , , cord-001257-t21l6i3f 65 15 which which WDT cord-001257-t21l6i3f 65 16 is be VBZ cord-001257-t21l6i3f 65 17 very very RB cord-001257-t21l6i3f 65 18 closely closely RB cord-001257-t21l6i3f 65 19 related related JJ cord-001257-t21l6i3f 65 20 to to IN cord-001257-t21l6i3f 65 21 TBSV TBSV NNP cord-001257-t21l6i3f 65 22 ) ) -RRB- cord-001257-t21l6i3f 65 23 was be VBD cord-001257-t21l6i3f 65 24 also also RB cord-001257-t21l6i3f 65 25 robust robust JJ cord-001257-t21l6i3f 65 26 in in IN cord-001257-t21l6i3f 65 27 Nicotiana Nicotiana NNP cord-001257-t21l6i3f 65 28 benthamiana benthamiana NNP cord-001257-t21l6i3f 65 29 host host NN cord-001257-t21l6i3f 65 30 plants plant NNS cord-001257-t21l6i3f 65 31 when when WRB cord-001257-t21l6i3f 65 32 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 65 33 and and CC cord-001257-t21l6i3f 65 34 AtRH5 AtRH5 NNP cord-001257-t21l6i3f 65 35 RNA RNA NNP cord-001257-t21l6i3f 65 36 helicases helicase NNS cord-001257-t21l6i3f 65 37 were be VBD cord-001257-t21l6i3f 65 38 overexpressed overexpresse VBN cord-001257-t21l6i3f 65 39 ( ( -LRB- cord-001257-t21l6i3f 65 40 up up IN cord-001257-t21l6i3f 65 41 to to IN cord-001257-t21l6i3f 65 42 , , , cord-001257-t21l6i3f 66 1 2- 2- LS cord-001257-t21l6i3f 66 2 Genome genome NN cord-001257-t21l6i3f 66 3 - - HYPH cord-001257-t21l6i3f 66 4 wide wide JJ cord-001257-t21l6i3f 66 5 screens screen NNS cord-001257-t21l6i3f 66 6 for for IN cord-001257-t21l6i3f 66 7 host host NN cord-001257-t21l6i3f 66 8 factors factor NNS cord-001257-t21l6i3f 66 9 affecting affect VBG cord-001257-t21l6i3f 66 10 tombusvirus tombusvirus NN cord-001257-t21l6i3f 66 11 replication replication NN cord-001257-t21l6i3f 66 12 in in IN cord-001257-t21l6i3f 66 13 yeast yeast NN cord-001257-t21l6i3f 66 14 indicated indicate VBD cord-001257-t21l6i3f 66 15 that that IN cord-001257-t21l6i3f 66 16 subverted subvert VBD cord-001257-t21l6i3f 66 17 cellular cellular JJ cord-001257-t21l6i3f 66 18 RNA RNA NNP cord-001257-t21l6i3f 66 19 helicases helicase NNS cord-001257-t21l6i3f 66 20 likely likely RB cord-001257-t21l6i3f 66 21 play play VBP cord-001257-t21l6i3f 66 22 major major JJ cord-001257-t21l6i3f 66 23 roles role NNS cord-001257-t21l6i3f 66 24 in in IN cord-001257-t21l6i3f 66 25 virus virus NN cord-001257-t21l6i3f 66 26 replication replication NN cord-001257-t21l6i3f 66 27 . . . cord-001257-t21l6i3f 67 1 Tombusviruses tombusviruse NNS cord-001257-t21l6i3f 67 2 do do VBP cord-001257-t21l6i3f 67 3 not not RB cord-001257-t21l6i3f 67 4 code code VB cord-001257-t21l6i3f 67 5 for for IN cord-001257-t21l6i3f 67 6 their -PRON- PRP$ cord-001257-t21l6i3f 67 7 own own JJ cord-001257-t21l6i3f 67 8 helicases helicase NNS cord-001257-t21l6i3f 67 9 and and CC cord-001257-t21l6i3f 67 10 they -PRON- PRP cord-001257-t21l6i3f 67 11 might may MD cord-001257-t21l6i3f 67 12 recruit recruit VB cord-001257-t21l6i3f 67 13 host host NN cord-001257-t21l6i3f 67 14 RNA RNA NNP cord-001257-t21l6i3f 67 15 helicases helicase NNS cord-001257-t21l6i3f 67 16 to to TO cord-001257-t21l6i3f 67 17 aid aid VB cord-001257-t21l6i3f 67 18 their -PRON- PRP$ cord-001257-t21l6i3f 67 19 replication replication NN cord-001257-t21l6i3f 67 20 in in IN cord-001257-t21l6i3f 67 21 infected infected JJ cord-001257-t21l6i3f 67 22 cells cell NNS cord-001257-t21l6i3f 67 23 . . . cord-001257-t21l6i3f 68 1 Accordingly accordingly RB cord-001257-t21l6i3f 68 2 , , , cord-001257-t21l6i3f 68 3 in in IN cord-001257-t21l6i3f 68 4 this this DT cord-001257-t21l6i3f 68 5 paper paper NN cord-001257-t21l6i3f 68 6 , , , cord-001257-t21l6i3f 68 7 the the DT cord-001257-t21l6i3f 68 8 authors author NNS cord-001257-t21l6i3f 68 9 show show VBP cord-001257-t21l6i3f 68 10 that that IN cord-001257-t21l6i3f 68 11 the the DT cord-001257-t21l6i3f 68 12 yeast yeast NN cord-001257-t21l6i3f 68 13 eIF4AIII eIF4AIII '' cord-001257-t21l6i3f 68 14 - - : cord-001257-t21l6i3f 68 15 like like IN cord-001257-t21l6i3f 68 16 Fal1p Fal1p NNP cord-001257-t21l6i3f 68 17 and and CC cord-001257-t21l6i3f 68 18 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 68 19 and and CC cord-001257-t21l6i3f 68 20 the the DT cord-001257-t21l6i3f 68 21 orthologous orthologous JJ cord-001257-t21l6i3f 68 22 plant plant NN cord-001257-t21l6i3f 68 23 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 68 24 and and CC cord-001257-t21l6i3f 68 25 AtRH5 AtRH5 NNP cord-001257-t21l6i3f 68 26 DEAD DEAD NNP cord-001257-t21l6i3f 68 27 - - HYPH cord-001257-t21l6i3f 68 28 box box NN cord-001257-t21l6i3f 68 29 helicases helicase NNS cord-001257-t21l6i3f 68 30 are be VBP cord-001257-t21l6i3f 68 31 co co VBN cord-001257-t21l6i3f 68 32 - - VBN cord-001257-t21l6i3f 68 33 opted opt VBN cord-001257-t21l6i3f 68 34 by by IN cord-001257-t21l6i3f 68 35 Tomato Tomato NNP cord-001257-t21l6i3f 68 36 bushy bushy JJ cord-001257-t21l6i3f 68 37 stunt stunt NN cord-001257-t21l6i3f 68 38 virus virus NN cord-001257-t21l6i3f 68 39 ( ( -LRB- cord-001257-t21l6i3f 68 40 TBSV TBSV NNP cord-001257-t21l6i3f 68 41 ) ) -RRB- cord-001257-t21l6i3f 68 42 to to TO cord-001257-t21l6i3f 68 43 aid aid VB cord-001257-t21l6i3f 68 44 viral viral JJ cord-001257-t21l6i3f 68 45 replication replication NN cord-001257-t21l6i3f 68 46 . . . cord-001257-t21l6i3f 69 1 The the DT cord-001257-t21l6i3f 69 2 authors author NNS cord-001257-t21l6i3f 69 3 find find VBP cord-001257-t21l6i3f 69 4 that that IN cord-001257-t21l6i3f 69 5 eIF4AIII eIF4AIII NNS cord-001257-t21l6i3f 69 6 - - HYPH cord-001257-t21l6i3f 69 7 like like JJ cord-001257-t21l6i3f 69 8 helicases helicase NNS cord-001257-t21l6i3f 69 9 bind bind VBP cord-001257-t21l6i3f 69 10 to to IN cord-001257-t21l6i3f 69 11 the the DT cord-001257-t21l6i3f 69 12 replication replication NN cord-001257-t21l6i3f 69 13 enhancer enhancer NN cord-001257-t21l6i3f 69 14 element element NN cord-001257-t21l6i3f 69 15 ( ( -LRB- cord-001257-t21l6i3f 69 16 REN REN NNP cord-001257-t21l6i3f 69 17 ) ) -RRB- cord-001257-t21l6i3f 69 18 in in IN cord-001257-t21l6i3f 69 19 the the DT cord-001257-t21l6i3f 69 20 viral viral NN cord-001257-t21l6i3f 69 21 ( ( -LRB- cord-001257-t21l6i3f 69 22 2)RNA 2)rna CD cord-001257-t21l6i3f 70 1 and and CC cord-001257-t21l6i3f 70 2 they -PRON- PRP cord-001257-t21l6i3f 70 3 promote promote VBP cord-001257-t21l6i3f 70 4 ( ( -LRB- cord-001257-t21l6i3f 70 5 + + . cord-001257-t21l6i3f 71 1 ) ) -RRB- cord-001257-t21l6i3f 71 2 strand strand NN cord-001257-t21l6i3f 71 3 TBSV TBSV NNP cord-001257-t21l6i3f 71 4 RNA RNA NNP cord-001257-t21l6i3f 71 5 synthesis synthesis NN cord-001257-t21l6i3f 71 6 in in IN cord-001257-t21l6i3f 71 7 vitro vitro FW cord-001257-t21l6i3f 71 8 . . . cord-001257-t21l6i3f 72 1 Data datum NNS cord-001257-t21l6i3f 72 2 show show VBP cord-001257-t21l6i3f 72 3 that that IN cord-001257-t21l6i3f 72 4 eIF4AIIIlike eif4aiiilike JJ cord-001257-t21l6i3f 72 5 helicases helicase NNS cord-001257-t21l6i3f 72 6 are be VBP cord-001257-t21l6i3f 72 7 present present JJ cord-001257-t21l6i3f 72 8 in in IN cord-001257-t21l6i3f 72 9 the the DT cord-001257-t21l6i3f 72 10 viral viral JJ cord-001257-t21l6i3f 72 11 replicase replicase NN cord-001257-t21l6i3f 72 12 complex complex NN cord-001257-t21l6i3f 72 13 and and CC cord-001257-t21l6i3f 72 14 they -PRON- PRP cord-001257-t21l6i3f 72 15 bind bind VBP cord-001257-t21l6i3f 72 16 to to IN cord-001257-t21l6i3f 72 17 the the DT cord-001257-t21l6i3f 72 18 replication replication NN cord-001257-t21l6i3f 72 19 proteins protein NNS cord-001257-t21l6i3f 72 20 . . . cord-001257-t21l6i3f 73 1 In in IN cord-001257-t21l6i3f 73 2 addition addition NN cord-001257-t21l6i3f 73 3 , , , cord-001257-t21l6i3f 73 4 the the DT cord-001257-t21l6i3f 73 5 authors author NNS cord-001257-t21l6i3f 73 6 show show VBP cord-001257-t21l6i3f 73 7 synergistic synergistic JJ cord-001257-t21l6i3f 73 8 effect effect NN cord-001257-t21l6i3f 73 9 between between IN cord-001257-t21l6i3f 73 10 eIF4AIII eIF4AIII NNS cord-001257-t21l6i3f 73 11 - - HYPH cord-001257-t21l6i3f 73 12 like like JJ cord-001257-t21l6i3f 73 13 helicases helicase NNS cord-001257-t21l6i3f 73 14 and and CC cord-001257-t21l6i3f 73 15 the the DT cord-001257-t21l6i3f 73 16 previously previously RB cord-001257-t21l6i3f 73 17 identified identify VBN cord-001257-t21l6i3f 73 18 DDX3-like DDX3-like NNP cord-001257-t21l6i3f 73 19 Ded1p/ ded1p/ DT cord-001257-t21l6i3f 73 20 AtRH20 atrh20 NN cord-001257-t21l6i3f 73 21 DEAD dead JJ cord-001257-t21l6i3f 73 22 box box NN cord-001257-t21l6i3f 73 23 helicases helicase NNS cord-001257-t21l6i3f 73 24 , , , cord-001257-t21l6i3f 73 25 which which WDT cord-001257-t21l6i3f 73 26 bind bind VBP cord-001257-t21l6i3f 73 27 to to IN cord-001257-t21l6i3f 73 28 a a DT cord-001257-t21l6i3f 73 29 different different JJ cord-001257-t21l6i3f 73 30 cisacting cisacte VBG cord-001257-t21l6i3f 73 31 region region NN cord-001257-t21l6i3f 73 32 in in IN cord-001257-t21l6i3f 73 33 the the DT cord-001257-t21l6i3f 73 34 viral viral NN cord-001257-t21l6i3f 73 35 ( ( -LRB- cord-001257-t21l6i3f 73 36 2)RNA 2)rna CD cord-001257-t21l6i3f 73 37 , , , cord-001257-t21l6i3f 73 38 on on IN cord-001257-t21l6i3f 73 39 stimulation stimulation NN cord-001257-t21l6i3f 73 40 of of IN cord-001257-t21l6i3f 73 41 plusstrand plusstrand NN cord-001257-t21l6i3f 73 42 synthesis synthesis NN cord-001257-t21l6i3f 73 43 . . . cord-001257-t21l6i3f 74 1 In in IN cord-001257-t21l6i3f 74 2 summary summary NN cord-001257-t21l6i3f 74 3 , , , cord-001257-t21l6i3f 74 4 the the DT cord-001257-t21l6i3f 74 5 authors author NNS cord-001257-t21l6i3f 74 6 find find VBP cord-001257-t21l6i3f 74 7 that that IN cord-001257-t21l6i3f 74 8 two two CD cord-001257-t21l6i3f 74 9 different different JJ cord-001257-t21l6i3f 74 10 groups group NNS cord-001257-t21l6i3f 74 11 of of IN cord-001257-t21l6i3f 74 12 cellular cellular JJ cord-001257-t21l6i3f 74 13 helicases helicase NNS cord-001257-t21l6i3f 74 14 promote promote VBP cord-001257-t21l6i3f 74 15 TBSV TBSV NNP cord-001257-t21l6i3f 74 16 replication replication NN cord-001257-t21l6i3f 74 17 via via IN cord-001257-t21l6i3f 74 18 selectively selectively RB cord-001257-t21l6i3f 74 19 enhancing enhance VBG cord-001257-t21l6i3f 74 20 ( ( -LRB- cord-001257-t21l6i3f 74 21 + + NNS cord-001257-t21l6i3f 74 22 ) ) -RRB- cord-001257-t21l6i3f 74 23 -strand -strand CD cord-001257-t21l6i3f 74 24 synthesis synthesis NN cord-001257-t21l6i3f 74 25 through through IN cord-001257-t21l6i3f 74 26 different different JJ cord-001257-t21l6i3f 74 27 mechanisms mechanism NNS cord-001257-t21l6i3f 74 28 . . . cord-001257-t21l6i3f 75 1 fold fold VB cord-001257-t21l6i3f 75 2 increase increase NN cord-001257-t21l6i3f 75 3 in in IN cord-001257-t21l6i3f 75 4 tombusvirus tombusvirus JJ cord-001257-t21l6i3f 75 5 genomic genomic JJ cord-001257-t21l6i3f 75 6 RNA RNA NNP cord-001257-t21l6i3f 75 7 accumulation accumulation NN cord-001257-t21l6i3f 75 8 , , , cord-001257-t21l6i3f 75 9 and and CC cord-001257-t21l6i3f 75 10 , , , cord-001257-t21l6i3f 75 11 6-fold 6-fold CD cord-001257-t21l6i3f 75 12 increase increase NN cord-001257-t21l6i3f 75 13 in in IN cord-001257-t21l6i3f 75 14 subgenomic subgenomic JJ cord-001257-t21l6i3f 75 15 RNA2 RNA2 NNP cord-001257-t21l6i3f 75 16 accumulation accumulation NN cord-001257-t21l6i3f 75 17 , , , cord-001257-t21l6i3f 75 18 Fig Fig NNP cord-001257-t21l6i3f 75 19 . . . cord-001257-t21l6i3f 75 20 1B 1b CD cord-001257-t21l6i3f 75 21 , , . cord-001257-t21l6i3f 76 1 lanes lane NNS cord-001257-t21l6i3f 76 2 1 1 CD cord-001257-t21l6i3f 76 3 - - SYM cord-001257-t21l6i3f 76 4 4 4 CD cord-001257-t21l6i3f 76 5 and and CC cord-001257-t21l6i3f 76 6 9 9 CD cord-001257-t21l6i3f 76 7 - - SYM cord-001257-t21l6i3f 76 8 12 12 CD cord-001257-t21l6i3f 76 9 versus versus IN cord-001257-t21l6i3f 76 10 5 5 CD cord-001257-t21l6i3f 76 11 - - SYM cord-001257-t21l6i3f 76 12 8 8 CD cord-001257-t21l6i3f 76 13 ) ) -RRB- cord-001257-t21l6i3f 76 14 , , , cord-001257-t21l6i3f 76 15 suggesting suggest VBG cord-001257-t21l6i3f 76 16 that that IN cord-001257-t21l6i3f 76 17 these these DT cord-001257-t21l6i3f 76 18 cellular cellular JJ cord-001257-t21l6i3f 76 19 helicases helicase NNS cord-001257-t21l6i3f 76 20 are be VBP cord-001257-t21l6i3f 76 21 important important JJ cord-001257-t21l6i3f 76 22 host host NN cord-001257-t21l6i3f 76 23 factors factor NNS cord-001257-t21l6i3f 76 24 . . . cord-001257-t21l6i3f 77 1 While while IN cord-001257-t21l6i3f 77 2 overexpression overexpression NN cord-001257-t21l6i3f 77 3 of of IN cord-001257-t21l6i3f 77 4 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 77 5 and and CC cord-001257-t21l6i3f 77 6 AtRH5 AtRH5 NNP cord-001257-t21l6i3f 77 7 did do VBD cord-001257-t21l6i3f 77 8 not not RB cord-001257-t21l6i3f 77 9 affect affect VB cord-001257-t21l6i3f 77 10 the the DT cord-001257-t21l6i3f 77 11 phenotype phenotype NN cord-001257-t21l6i3f 77 12 of of IN cord-001257-t21l6i3f 77 13 uninoculated uninoculated NNP cord-001257-t21l6i3f 77 14 N. N. NNP cord-001257-t21l6i3f 77 15 benthamiana benthamiana NNP cord-001257-t21l6i3f 77 16 plants plant NNS cord-001257-t21l6i3f 77 17 , , , cord-001257-t21l6i3f 77 18 the the DT cord-001257-t21l6i3f 77 19 tombusvirus tombusvirus NN cord-001257-t21l6i3f 77 20 - - HYPH cord-001257-t21l6i3f 77 21 induced induce VBN cord-001257-t21l6i3f 77 22 symptoms symptom NNS cord-001257-t21l6i3f 77 23 were be VBD cord-001257-t21l6i3f 77 24 intensified intensify VBN cord-001257-t21l6i3f 77 25 ( ( -LRB- cord-001257-t21l6i3f 77 26 Fig Fig NNP cord-001257-t21l6i3f 77 27 . . NNP cord-001257-t21l6i3f 77 28 2 2 CD cord-001257-t21l6i3f 77 29 ) ) -RRB- cord-001257-t21l6i3f 77 30 and and CC cord-001257-t21l6i3f 77 31 the the DT cord-001257-t21l6i3f 77 32 symptoms symptom NNS cord-001257-t21l6i3f 77 33 appeared appear VBD cord-001257-t21l6i3f 77 34 faster fast RBR cord-001257-t21l6i3f 77 35 ( ( -LRB- cord-001257-t21l6i3f 77 36 not not RB cord-001257-t21l6i3f 77 37 shown show VBN cord-001257-t21l6i3f 77 38 ) ) -RRB- cord-001257-t21l6i3f 77 39 , , , cord-001257-t21l6i3f 77 40 when when WRB cord-001257-t21l6i3f 77 41 compared compare VBN cord-001257-t21l6i3f 77 42 with with IN cord-001257-t21l6i3f 77 43 the the DT cord-001257-t21l6i3f 77 44 control control NN cord-001257-t21l6i3f 77 45 host host NN cord-001257-t21l6i3f 77 46 plants plant NNS cord-001257-t21l6i3f 77 47 not not RB cord-001257-t21l6i3f 77 48 overexpressing overexpresse VBG cord-001257-t21l6i3f 77 49 the the DT cord-001257-t21l6i3f 77 50 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 77 51 and and CC cord-001257-t21l6i3f 77 52 AtRH5 atrh5 NN cord-001257-t21l6i3f 77 53 helicases helicase NNS cord-001257-t21l6i3f 77 54 . . . cord-001257-t21l6i3f 78 1 Simultaneous simultaneous JJ cord-001257-t21l6i3f 78 2 co co NN cord-001257-t21l6i3f 78 3 - - NN cord-001257-t21l6i3f 78 4 overexpression overexpression NN cord-001257-t21l6i3f 78 5 of of IN cord-001257-t21l6i3f 78 6 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 78 7 and and CC cord-001257-t21l6i3f 78 8 AtRH5 AtRH5 NNP cord-001257-t21l6i3f 78 9 increased increase VBD cord-001257-t21l6i3f 78 10 tombusvirus tombusvirus NN cord-001257-t21l6i3f 78 11 replication replication NN cord-001257-t21l6i3f 78 12 by by IN cord-001257-t21l6i3f 78 13 up up IN cord-001257-t21l6i3f 78 14 to to IN cord-001257-t21l6i3f 79 1 2-fold 2-fold LS cord-001257-t21l6i3f 79 2 ( ( -LRB- cord-001257-t21l6i3f 79 3 Fig Fig NNP cord-001257-t21l6i3f 79 4 . . . cord-001257-t21l6i3f 79 5 1B 1b CD cord-001257-t21l6i3f 79 6 , , , cord-001257-t21l6i3f 79 7 lanes lane VBZ cord-001257-t21l6i3f 79 8 13 13 CD cord-001257-t21l6i3f 79 9 - - SYM cord-001257-t21l6i3f 79 10 16 16 CD cord-001257-t21l6i3f 79 11 ) ) -RRB- cord-001257-t21l6i3f 79 12 , , , cord-001257-t21l6i3f 79 13 similar similar JJ cord-001257-t21l6i3f 79 14 to to IN cord-001257-t21l6i3f 79 15 the the DT cord-001257-t21l6i3f 79 16 level level NN cord-001257-t21l6i3f 79 17 obtained obtain VBN cord-001257-t21l6i3f 79 18 with with IN cord-001257-t21l6i3f 79 19 individual individual JJ cord-001257-t21l6i3f 79 20 overexpression overexpression NN cord-001257-t21l6i3f 79 21 of of IN cord-001257-t21l6i3f 79 22 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 79 23 and and CC cord-001257-t21l6i3f 79 24 AtRH5 atrh5 NN cord-001257-t21l6i3f 79 25 . . . cord-001257-t21l6i3f 80 1 Also also RB cord-001257-t21l6i3f 80 2 , , , cord-001257-t21l6i3f 80 3 the the DT cord-001257-t21l6i3f 80 4 symptoms symptom NNS cord-001257-t21l6i3f 80 5 induced induce VBN cord-001257-t21l6i3f 80 6 by by IN cord-001257-t21l6i3f 80 7 tombusvirus tombusvirus JJ cord-001257-t21l6i3f 80 8 infection infection NN cord-001257-t21l6i3f 80 9 in in IN cord-001257-t21l6i3f 80 10 plants plant NNS cord-001257-t21l6i3f 80 11 with with IN cord-001257-t21l6i3f 80 12 cooverexpression cooverexpression NN cord-001257-t21l6i3f 80 13 of of IN cord-001257-t21l6i3f 80 14 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 80 15 and and CC cord-001257-t21l6i3f 80 16 AtRH5 AtRH5 NNP cord-001257-t21l6i3f 80 17 were be VBD cord-001257-t21l6i3f 80 18 comparable comparable JJ cord-001257-t21l6i3f 80 19 to to IN cord-001257-t21l6i3f 80 20 those those DT cord-001257-t21l6i3f 80 21 induced induce VBN cord-001257-t21l6i3f 80 22 by by IN cord-001257-t21l6i3f 80 23 the the DT cord-001257-t21l6i3f 80 24 individual individual JJ cord-001257-t21l6i3f 80 25 overexpression overexpression NN cord-001257-t21l6i3f 80 26 of of IN cord-001257-t21l6i3f 80 27 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 80 28 and and CC cord-001257-t21l6i3f 80 29 AtRH5 atrh5 NN cord-001257-t21l6i3f 81 1 ( ( -LRB- cord-001257-t21l6i3f 81 2 Fig Fig NNP cord-001257-t21l6i3f 81 3 . . NNP cord-001257-t21l6i3f 81 4 2 2 CD cord-001257-t21l6i3f 81 5 ) ) -RRB- cord-001257-t21l6i3f 81 6 . . . cord-001257-t21l6i3f 82 1 Therefore therefore RB cord-001257-t21l6i3f 82 2 , , , cord-001257-t21l6i3f 82 3 it -PRON- PRP cord-001257-t21l6i3f 82 4 is be VBZ cord-001257-t21l6i3f 82 5 likely likely JJ cord-001257-t21l6i3f 82 6 that that DT cord-001257-t21l6i3f 82 7 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 82 8 and and CC cord-001257-t21l6i3f 82 9 AtRH5 atrh5 NN cord-001257-t21l6i3f 82 10 play play VBP cord-001257-t21l6i3f 82 11 comparable comparable JJ cord-001257-t21l6i3f 82 12 and and CC cord-001257-t21l6i3f 82 13 overlapping overlapping JJ cord-001257-t21l6i3f 82 14 functions function NNS cord-001257-t21l6i3f 82 15 in in IN cord-001257-t21l6i3f 82 16 tombusvirus tombusvirus NN cord-001257-t21l6i3f 82 17 replication replication NN cord-001257-t21l6i3f 82 18 . . . cord-001257-t21l6i3f 83 1 Overall overall RB cord-001257-t21l6i3f 83 2 , , , cord-001257-t21l6i3f 83 3 the the DT cord-001257-t21l6i3f 83 4 host host NN cord-001257-t21l6i3f 83 5 helicase helicase NN cord-001257-t21l6i3f 83 6 overexpression overexpression NN cord-001257-t21l6i3f 83 7 studies study NNS cord-001257-t21l6i3f 83 8 in in IN cord-001257-t21l6i3f 83 9 yeast yeast NN cord-001257-t21l6i3f 83 10 and and CC cord-001257-t21l6i3f 83 11 plant plant NN cord-001257-t21l6i3f 83 12 established establish VBN cord-001257-t21l6i3f 83 13 that that IN cord-001257-t21l6i3f 83 14 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 83 15 and and CC cord-001257-t21l6i3f 83 16 AtRH5 atrh5 NN cord-001257-t21l6i3f 83 17 helicases helicase NNS cord-001257-t21l6i3f 83 18 and and CC cord-001257-t21l6i3f 83 19 the the DT cord-001257-t21l6i3f 83 20 orthologous orthologous NNP cord-001257-t21l6i3f 83 21 yeast yeast NN cord-001257-t21l6i3f 83 22 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 83 23 and and CC cord-001257-t21l6i3f 83 24 Fal1p Fal1p NNP cord-001257-t21l6i3f 83 25 helicases helicase NNS cord-001257-t21l6i3f 83 26 could could MD cord-001257-t21l6i3f 83 27 support support VB cord-001257-t21l6i3f 83 28 increased increase VBN cord-001257-t21l6i3f 83 29 level level NN cord-001257-t21l6i3f 83 30 of of IN cord-001257-t21l6i3f 83 31 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 83 32 RNA RNA NNP cord-001257-t21l6i3f 83 33 replication replication NN cord-001257-t21l6i3f 83 34 in in IN cord-001257-t21l6i3f 83 35 host host NN cord-001257-t21l6i3f 83 36 cells cell NNS cord-001257-t21l6i3f 83 37 . . . cord-001257-t21l6i3f 84 1 Novel novel JJ cord-001257-t21l6i3f 84 2 pro pro JJ cord-001257-t21l6i3f 84 3 - - JJ cord-001257-t21l6i3f 84 4 viral viral JJ cord-001257-t21l6i3f 84 5 function function NN cord-001257-t21l6i3f 84 6 of of IN cord-001257-t21l6i3f 84 7 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 84 8 , , , cord-001257-t21l6i3f 84 9 AtRH5 atrh5 NN cord-001257-t21l6i3f 84 10 and and CC cord-001257-t21l6i3f 84 11 the the DT cord-001257-t21l6i3f 84 12 yeast yeast NN cord-001257-t21l6i3f 84 13 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 84 14 and and CC cord-001257-t21l6i3f 84 15 Fal1p Fal1p NNP cord-001257-t21l6i3f 84 16 is be VBZ cord-001257-t21l6i3f 84 17 to to TO cord-001257-t21l6i3f 84 18 bind bind VB cord-001257-t21l6i3f 84 19 to to IN cord-001257-t21l6i3f 84 20 the the DT cord-001257-t21l6i3f 84 21 viral viral JJ cord-001257-t21l6i3f 84 22 replication replication NN cord-001257-t21l6i3f 84 23 enhancer enhancer NN cord-001257-t21l6i3f 84 24 present present JJ cord-001257-t21l6i3f 84 25 in in IN cord-001257-t21l6i3f 84 26 the the DT cord-001257-t21l6i3f 84 27 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 84 28 minus minus JJ cord-001257-t21l6i3f 84 29 - - HYPH cord-001257-t21l6i3f 84 30 strand strand NN cord-001257-t21l6i3f 84 31 RNA RNA NNP cord-001257-t21l6i3f 84 32 To to TO cord-001257-t21l6i3f 84 33 test test VB cord-001257-t21l6i3f 84 34 if if IN cord-001257-t21l6i3f 84 35 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 84 36 , , , cord-001257-t21l6i3f 84 37 AtRH5 atrh5 NN cord-001257-t21l6i3f 84 38 and and CC cord-001257-t21l6i3f 84 39 the the DT cord-001257-t21l6i3f 84 40 yeast yeast NN cord-001257-t21l6i3f 84 41 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 84 42 and and CC cord-001257-t21l6i3f 84 43 Fal1p Fal1p NNP cord-001257-t21l6i3f 84 44 play play VBP cord-001257-t21l6i3f 84 45 a a DT cord-001257-t21l6i3f 84 46 comparable comparable JJ cord-001257-t21l6i3f 84 47 role role NN cord-001257-t21l6i3f 84 48 with with IN cord-001257-t21l6i3f 84 49 the the DT cord-001257-t21l6i3f 84 50 previously previously RB cord-001257-t21l6i3f 84 51 analyzed analyze VBN cord-001257-t21l6i3f 84 52 yeast yeast NN cord-001257-t21l6i3f 84 53 Ded1p ded1p IN cord-001257-t21l6i3f 84 54 ( ( -LRB- cord-001257-t21l6i3f 84 55 human human JJ cord-001257-t21l6i3f 84 56 DDX3-like ddx3-like NN cord-001257-t21l6i3f 84 57 ) ) -RRB- cord-001257-t21l6i3f 85 1 and and CC cord-001257-t21l6i3f 85 2 Dbp2p Dbp2p NNP cord-001257-t21l6i3f 85 3 ( ( -LRB- cord-001257-t21l6i3f 85 4 human human NNP cord-001257-t21l6i3f 85 5 p68-like p68-like NNP cord-001257-t21l6i3f 85 6 ) ) -RRB- cord-001257-t21l6i3f 85 7 and and CC cord-001257-t21l6i3f 85 8 the the DT cord-001257-t21l6i3f 85 9 ortologous ortologous JJ cord-001257-t21l6i3f 85 10 AtRH20 AtRH20 NNP cord-001257-t21l6i3f 85 11 DEAD DEAD NNP cord-001257-t21l6i3f 85 12 - - HYPH cord-001257-t21l6i3f 85 13 box box NN cord-001257-t21l6i3f 85 14 helicases helicase NNS cord-001257-t21l6i3f 85 15 [ [ -LRB- cord-001257-t21l6i3f 85 16 30 30 CD cord-001257-t21l6i3f 85 17 , , , cord-001257-t21l6i3f 85 18 49 49 CD cord-001257-t21l6i3f 85 19 ] ] -RRB- cord-001257-t21l6i3f 85 20 , , , cord-001257-t21l6i3f 85 21 first first RB cord-001257-t21l6i3f 85 22 we -PRON- PRP cord-001257-t21l6i3f 85 23 performed perform VBD cord-001257-t21l6i3f 85 24 in in IN cord-001257-t21l6i3f 85 25 vitro vitro FW cord-001257-t21l6i3f 85 26 RNA rna NN cord-001257-t21l6i3f 85 27 binding bind VBG cord-001257-t21l6i3f 85 28 experiments experiment NNS cord-001257-t21l6i3f 85 29 with with IN cord-001257-t21l6i3f 85 30 affinity affinity NN cord-001257-t21l6i3f 85 31 - - HYPH cord-001257-t21l6i3f 85 32 purified purify VBN cord-001257-t21l6i3f 85 33 recombinant recombinant NN cord-001257-t21l6i3f 85 34 helicase helicase NN cord-001257-t21l6i3f 85 35 proteins protein NNS cord-001257-t21l6i3f 85 36 . . . cord-001257-t21l6i3f 86 1 Using use VBG cord-001257-t21l6i3f 86 2 four four CD cord-001257-t21l6i3f 86 3 different different JJ cord-001257-t21l6i3f 86 4 cis cis NN cord-001257-t21l6i3f 86 5 - - HYPH cord-001257-t21l6i3f 86 6 acting act VBG cord-001257-t21l6i3f 86 7 regions region NNS cord-001257-t21l6i3f 86 8 present present JJ cord-001257-t21l6i3f 86 9 in in IN cord-001257-t21l6i3f 86 10 the the DT cord-001257-t21l6i3f 86 11 TBSV TBSV NNP cord-001257-t21l6i3f 86 12 ( ( -LRB- cord-001257-t21l6i3f 86 13 2)repRNA 2)repRNA NNP cord-001257-t21l6i3f 86 14 ( ( -LRB- cord-001257-t21l6i3f 86 15 Fig Fig NNP cord-001257-t21l6i3f 86 16 . . . cord-001257-t21l6i3f 86 17 3A 3A NNP cord-001257-t21l6i3f 86 18 ) ) -RRB- cord-001257-t21l6i3f 86 19 , , , cord-001257-t21l6i3f 86 20 we -PRON- PRP cord-001257-t21l6i3f 86 21 found find VBD cord-001257-t21l6i3f 86 22 that that IN cord-001257-t21l6i3f 86 23 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 86 24 and and CC cord-001257-t21l6i3f 86 25 AtRH5 atrh5 NN cord-001257-t21l6i3f 86 26 bind bind VB cord-001257-t21l6i3f 86 27 to to IN cord-001257-t21l6i3f 86 28 a a DT cord-001257-t21l6i3f 86 29 unique unique JJ cord-001257-t21l6i3f 86 30 cis cis NN cord-001257-t21l6i3f 86 31 - - HYPH cord-001257-t21l6i3f 86 32 acting act VBG cord-001257-t21l6i3f 86 33 sequence sequence NN cord-001257-t21l6i3f 86 34 in in IN cord-001257-t21l6i3f 86 35 a a DT cord-001257-t21l6i3f 86 36 59 59 CD cord-001257-t21l6i3f 86 37 proximal proximal JJ cord-001257-t21l6i3f 86 38 region region NN cord-001257-t21l6i3f 86 39 of of IN cord-001257-t21l6i3f 86 40 the the DT cord-001257-t21l6i3f 86 41 minus minus JJ cord-001257-t21l6i3f 86 42 - - HYPH cord-001257-t21l6i3f 86 43 strand strand NN cord-001257-t21l6i3f 86 44 RNA RNA NNP cord-001257-t21l6i3f 86 45 , , , cord-001257-t21l6i3f 86 46 called call VBN cord-001257-t21l6i3f 86 47 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 86 48 ) ) -RRB- cord-001257-t21l6i3f 86 49 , , , cord-001257-t21l6i3f 86 50 which which WDT cord-001257-t21l6i3f 86 51 carries carry VBZ cord-001257-t21l6i3f 86 52 a a DT cord-001257-t21l6i3f 86 53 well well RB cord-001257-t21l6i3f 86 54 - - HYPH cord-001257-t21l6i3f 86 55 defined define VBN cord-001257-t21l6i3f 86 56 RNA RNA NNP cord-001257-t21l6i3f 86 57 replication replication NN cord-001257-t21l6i3f 86 58 enhancer enhancer NN cord-001257-t21l6i3f 86 59 ( ( -LRB- cord-001257-t21l6i3f 86 60 REN REN NNP cord-001257-t21l6i3f 86 61 ) ) -RRB- cord-001257-t21l6i3f 86 62 element element NN cord-001257-t21l6i3f 86 63 ( ( -LRB- cord-001257-t21l6i3f 86 64 Fig Fig NNP cord-001257-t21l6i3f 86 65 . . . cord-001257-t21l6i3f 86 66 3B 3B NNP cord-001257-t21l6i3f 86 67 , , , cord-001257-t21l6i3f 86 68 lanes lane VBZ cord-001257-t21l6i3f 86 69 6 6 CD cord-001257-t21l6i3f 86 70 - - SYM cord-001257-t21l6i3f 86 71 7 7 CD cord-001257-t21l6i3f 86 72 ; ; , cord-001257-t21l6i3f 86 73 and and CC cord-001257-t21l6i3f 86 74 S2A S2A NNP cord-001257-t21l6i3f 86 75 , , , cord-001257-t21l6i3f 86 76 lanes lane NNS cord-001257-t21l6i3f 86 77 5 5 CD cord-001257-t21l6i3f 86 78 - - SYM cord-001257-t21l6i3f 86 79 6 6 CD cord-001257-t21l6i3f 86 80 ) ) -RRB- cord-001257-t21l6i3f 86 81 [ [ -LRB- cord-001257-t21l6i3f 86 82 55 55 CD cord-001257-t21l6i3f 86 83 , , , cord-001257-t21l6i3f 86 84 56 56 CD cord-001257-t21l6i3f 86 85 ] ] -RRB- cord-001257-t21l6i3f 86 86 . . . cord-001257-t21l6i3f 87 1 The the DT cord-001257-t21l6i3f 87 2 recombinant recombinant JJ cord-001257-t21l6i3f 87 3 yeast yeast NN cord-001257-t21l6i3f 87 4 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 87 5 and and CC cord-001257-t21l6i3f 87 6 Fal1p Fal1p NNP cord-001257-t21l6i3f 87 7 RNA RNA NNP cord-001257-t21l6i3f 87 8 helicases helicase NNS cord-001257-t21l6i3f 87 9 showed show VBD cord-001257-t21l6i3f 87 10 similar similar JJ cord-001257-t21l6i3f 87 11 RNA RNA NNP cord-001257-t21l6i3f 87 12 binding bind VBG cord-001257-t21l6i3f 87 13 characteristics characteristic NNS cord-001257-t21l6i3f 87 14 to to IN cord-001257-t21l6i3f 87 15 the the DT cord-001257-t21l6i3f 87 16 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 87 17 ) ) -RRB- cord-001257-t21l6i3f 87 18 REN REN NNP cord-001257-t21l6i3f 87 19 in in IN cord-001257-t21l6i3f 87 20 vitro vitro FW cord-001257-t21l6i3f 87 21 ( ( -LRB- cord-001257-t21l6i3f 87 22 Fig fig NN cord-001257-t21l6i3f 87 23 . . . cord-001257-t21l6i3f 88 1 S2B S2B NNS cord-001257-t21l6i3f 88 2 - - HYPH cord-001257-t21l6i3f 88 3 C C NNP cord-001257-t21l6i3f 88 4 ) ) -RRB- cord-001257-t21l6i3f 88 5 . . . cord-001257-t21l6i3f 89 1 Importantly importantly RB cord-001257-t21l6i3f 89 2 , , , cord-001257-t21l6i3f 89 3 binding bind VBG cord-001257-t21l6i3f 89 4 of of IN cord-001257-t21l6i3f 89 5 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 89 6 , , , cord-001257-t21l6i3f 89 7 AtRH5 atrh5 NN cord-001257-t21l6i3f 89 8 and and CC cord-001257-t21l6i3f 89 9 the the DT cord-001257-t21l6i3f 89 10 yeast yeast NN cord-001257-t21l6i3f 89 11 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 89 12 and and CC cord-001257-t21l6i3f 89 13 Fal1p Fal1p NNP cord-001257-t21l6i3f 89 14 to to IN cord-001257-t21l6i3f 89 15 the the DT cord-001257-t21l6i3f 89 16 TBSV TBSV NNP cord-001257-t21l6i3f 89 17 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 89 18 ) ) -RRB- cord-001257-t21l6i3f 90 1 REN REN NNP cord-001257-t21l6i3f 90 2 element element NN cord-001257-t21l6i3f 90 3 is be VBZ cord-001257-t21l6i3f 90 4 a a DT cord-001257-t21l6i3f 90 5 novel novel JJ cord-001257-t21l6i3f 90 6 feature feature NN cord-001257-t21l6i3f 90 7 for for IN cord-001257-t21l6i3f 90 8 co co JJ cord-001257-t21l6i3f 90 9 - - JJ cord-001257-t21l6i3f 90 10 opted opt VBN cord-001257-t21l6i3f 90 11 host host NN cord-001257-t21l6i3f 90 12 helicases helicase NNS cord-001257-t21l6i3f 90 13 . . . cord-001257-t21l6i3f 91 1 Indeed indeed RB cord-001257-t21l6i3f 91 2 , , , cord-001257-t21l6i3f 91 3 the the DT cord-001257-t21l6i3f 91 4 previously previously RB cord-001257-t21l6i3f 91 5 characterized characterize VBN cord-001257-t21l6i3f 91 6 AtRH20 atrh20 NN cord-001257-t21l6i3f 91 7 and and CC cord-001257-t21l6i3f 91 8 the the DT cord-001257-t21l6i3f 91 9 yeast yeast NN cord-001257-t21l6i3f 91 10 Ded1p ded1p NN cord-001257-t21l6i3f 91 11 and and CC cord-001257-t21l6i3f 91 12 Dbp2p Dbp2p NNP cord-001257-t21l6i3f 91 13 DEAD DEAD NNP cord-001257-t21l6i3f 91 14 - - HYPH cord-001257-t21l6i3f 91 15 box box NN cord-001257-t21l6i3f 91 16 helicases helicase NNS cord-001257-t21l6i3f 91 17 bound bind VBD cord-001257-t21l6i3f 91 18 the the DT cord-001257-t21l6i3f 91 19 most most RBS cord-001257-t21l6i3f 91 20 efficiently efficiently RB cord-001257-t21l6i3f 91 21 to to IN cord-001257-t21l6i3f 91 22 RI(2 RI(2 NNP cord-001257-t21l6i3f 91 23 ) ) -RRB- cord-001257-t21l6i3f 91 24 sequence sequence NN cord-001257-t21l6i3f 91 25 carrying carry VBG cord-001257-t21l6i3f 91 26 the the DT cord-001257-t21l6i3f 91 27 plusstrand plusstrand NN cord-001257-t21l6i3f 91 28 initiation initiation NN cord-001257-t21l6i3f 91 29 promoter promoter NN cord-001257-t21l6i3f 91 30 ( ( -LRB- cord-001257-t21l6i3f 91 31 Fig Fig NNP cord-001257-t21l6i3f 91 32 . . . cord-001257-t21l6i3f 91 33 3A 3a NN cord-001257-t21l6i3f 91 34 ; ; : cord-001257-t21l6i3f 91 35 Fig Fig NNP cord-001257-t21l6i3f 91 36 . . . cord-001257-t21l6i3f 91 37 3D 3D NNP cord-001257-t21l6i3f 91 38 versus versus IN cord-001257-t21l6i3f 91 39 3C 3C NNP cord-001257-t21l6i3f 91 40 ) ) -RRB- cord-001257-t21l6i3f 91 41 [ [ -LRB- cord-001257-t21l6i3f 91 42 30 30 CD cord-001257-t21l6i3f 91 43 , , , cord-001257-t21l6i3f 91 44 49 49 CD cord-001257-t21l6i3f 91 45 ] ] -RRB- cord-001257-t21l6i3f 91 46 . . . cord-001257-t21l6i3f 92 1 This this DT cord-001257-t21l6i3f 92 2 striking striking JJ cord-001257-t21l6i3f 92 3 difference difference NN cord-001257-t21l6i3f 92 4 in in IN cord-001257-t21l6i3f 92 5 recognition recognition NN cord-001257-t21l6i3f 92 6 of of IN cord-001257-t21l6i3f 92 7 two two CD cord-001257-t21l6i3f 92 8 separate separate JJ cord-001257-t21l6i3f 92 9 cis cis NN cord-001257-t21l6i3f 92 10 - - HYPH cord-001257-t21l6i3f 92 11 acting act VBG cord-001257-t21l6i3f 92 12 elements element NNS cord-001257-t21l6i3f 92 13 [ [ -LRB- cord-001257-t21l6i3f 92 14 indeed indeed RB cord-001257-t21l6i3f 92 15 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 92 16 ) ) -RRB- cord-001257-t21l6i3f 93 1 REN REN NNP cord-001257-t21l6i3f 93 2 is be VBZ cord-001257-t21l6i3f 93 3 located locate VBN cord-001257-t21l6i3f 93 4 close close RB cord-001257-t21l6i3f 93 5 to to IN cord-001257-t21l6i3f 93 6 the the DT cord-001257-t21l6i3f 93 7 59end 59end NN cord-001257-t21l6i3f 93 8 , , , cord-001257-t21l6i3f 93 9 while while IN cord-001257-t21l6i3f 93 10 RI(2 RI(2 NNP cord-001257-t21l6i3f 93 11 ) ) -RRB- cord-001257-t21l6i3f 93 12 promoter promoter NN cord-001257-t21l6i3f 93 13 region region NN cord-001257-t21l6i3f 93 14 is be VBZ cord-001257-t21l6i3f 93 15 situated situate VBN cord-001257-t21l6i3f 93 16 at at IN cord-001257-t21l6i3f 93 17 the the DT cord-001257-t21l6i3f 93 18 39 39 CD cord-001257-t21l6i3f 93 19 end end NN cord-001257-t21l6i3f 93 20 of of IN cord-001257-t21l6i3f 93 21 the the DT cord-001257-t21l6i3f 93 22 viral viral JJ cord-001257-t21l6i3f 93 23 ( ( -LRB- cord-001257-t21l6i3f 93 24 2 2 CD cord-001257-t21l6i3f 93 25 ) ) -RRB- cord-001257-t21l6i3f 93 26 RNA RNA NNP cord-001257-t21l6i3f 93 27 , , , cord-001257-t21l6i3f 93 28 Fig Fig NNP cord-001257-t21l6i3f 93 29 . . . cord-001257-t21l6i3f 94 1 3A 3a LS cord-001257-t21l6i3f 94 2 ] ] -RRB- cord-001257-t21l6i3f 94 3 by by IN cord-001257-t21l6i3f 94 4 these these DT cord-001257-t21l6i3f 94 5 host host NN cord-001257-t21l6i3f 94 6 helicases helicase NNS cord-001257-t21l6i3f 94 7 indicate indicate VBP cord-001257-t21l6i3f 94 8 that that IN cord-001257-t21l6i3f 94 9 their -PRON- PRP$ cord-001257-t21l6i3f 94 10 functions function NNS cord-001257-t21l6i3f 94 11 and and CC cord-001257-t21l6i3f 94 12 the the DT cord-001257-t21l6i3f 94 13 mechanism mechanism NN cord-001257-t21l6i3f 94 14 of of IN cord-001257-t21l6i3f 94 15 stimulation stimulation NN cord-001257-t21l6i3f 94 16 of of IN cord-001257-t21l6i3f 94 17 TBSV TBSV NNP cord-001257-t21l6i3f 94 18 RNA RNA NNP cord-001257-t21l6i3f 94 19 replication replication NN cord-001257-t21l6i3f 94 20 must must MD cord-001257-t21l6i3f 94 21 be be VB cord-001257-t21l6i3f 94 22 different different JJ cord-001257-t21l6i3f 94 23 . . . cord-001257-t21l6i3f 95 1 To to TO cord-001257-t21l6i3f 95 2 confirm confirm VB cord-001257-t21l6i3f 95 3 binding binding NN cord-001257-t21l6i3f 95 4 of of IN cord-001257-t21l6i3f 95 5 AtRH5 atrh5 NN cord-001257-t21l6i3f 95 6 to to IN cord-001257-t21l6i3f 95 7 the the DT cord-001257-t21l6i3f 95 8 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 95 9 ) ) -RRB- cord-001257-t21l6i3f 95 10 REN REN NNP cord-001257-t21l6i3f 95 11 , , , cord-001257-t21l6i3f 95 12 we -PRON- PRP cord-001257-t21l6i3f 95 13 also also RB cord-001257-t21l6i3f 95 14 performed perform VBD cord-001257-t21l6i3f 95 15 UV UV NNP cord-001257-t21l6i3f 95 16 cross cross JJ cord-001257-t21l6i3f 95 17 - - JJ cord-001257-t21l6i3f 95 18 linking link VBG cord-001257-t21l6i3f 95 19 experiments experiment NNS cord-001257-t21l6i3f 95 20 with with IN cord-001257-t21l6i3f 95 21 32 32 CD cord-001257-t21l6i3f 95 22 P p NN cord-001257-t21l6i3f 95 23 - - HYPH cord-001257-t21l6i3f 95 24 labeled label VBN cord-001257-t21l6i3f 95 25 DI-72(2 DI-72(2 NNP cord-001257-t21l6i3f 95 26 ) ) -RRB- cord-001257-t21l6i3f 95 27 RNA RNA NNP cord-001257-t21l6i3f 95 28 and and CC cord-001257-t21l6i3f 95 29 cold cold JJ cord-001257-t21l6i3f 95 30 competitors competitor NNS cord-001257-t21l6i3f 95 31 ( ( -LRB- cord-001257-t21l6i3f 95 32 Fig Fig NNP cord-001257-t21l6i3f 95 33 . . . cord-001257-t21l6i3f 95 34 3E 3E NNP cord-001257-t21l6i3f 95 35 ) ) -RRB- cord-001257-t21l6i3f 95 36 . . . cord-001257-t21l6i3f 96 1 The the DT cord-001257-t21l6i3f 96 2 82 82 CD cord-001257-t21l6i3f 96 3 nt nt RB cord-001257-t21l6i3f 96 4 complete complete JJ cord-001257-t21l6i3f 96 5 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 96 6 ) ) -RRB- cord-001257-t21l6i3f 97 1 REN REN NNP cord-001257-t21l6i3f 97 2 was be VBD cord-001257-t21l6i3f 97 3 a a DT cord-001257-t21l6i3f 97 4 better well JJR cord-001257-t21l6i3f 97 5 competitor competitor NN cord-001257-t21l6i3f 97 6 than than IN cord-001257-t21l6i3f 97 7 similar similar JJ cord-001257-t21l6i3f 97 8 - - HYPH cord-001257-t21l6i3f 97 9 sized sized JJ cord-001257-t21l6i3f 97 10 RNA RNA NNP cord-001257-t21l6i3f 97 11 lacking lack VBG cord-001257-t21l6i3f 97 12 one one CD cord-001257-t21l6i3f 97 13 of of IN cord-001257-t21l6i3f 97 14 the the DT cord-001257-t21l6i3f 97 15 stem stem NN cord-001257-t21l6i3f 97 16 - - HYPH cord-001257-t21l6i3f 97 17 loop loop NN cord-001257-t21l6i3f 97 18 structure structure NN cord-001257-t21l6i3f 97 19 and and CC cord-001257-t21l6i3f 98 1 a a DT cord-001257-t21l6i3f 98 2 ' ' `` cord-001257-t21l6i3f 98 3 ' ' `` cord-001257-t21l6i3f 98 4 bridge bridge NN cord-001257-t21l6i3f 98 5 ' ' '' cord-001257-t21l6i3f 98 6 ' ' '' cord-001257-t21l6i3f 98 7 sequence sequence NN cord-001257-t21l6i3f 98 8 that that WDT cord-001257-t21l6i3f 98 9 can can MD cord-001257-t21l6i3f 98 10 base base VB cord-001257-t21l6i3f 98 11 - - HYPH cord-001257-t21l6i3f 98 12 pair pair VB cord-001257-t21l6i3f 98 13 with with IN cord-001257-t21l6i3f 98 14 RI(2 ri(2 NN cord-001257-t21l6i3f 98 15 ) ) -RRB- cord-001257-t21l6i3f 98 16 sequence sequence NN cord-001257-t21l6i3f 98 17 via via IN cord-001257-t21l6i3f 98 18 long long JJ cord-001257-t21l6i3f 98 19 - - HYPH cord-001257-t21l6i3f 98 20 range range NN cord-001257-t21l6i3f 98 21 interaction interaction NN cord-001257-t21l6i3f 98 22 ( ( -LRB- cord-001257-t21l6i3f 98 23 Fig Fig NNP cord-001257-t21l6i3f 98 24 . . . cord-001257-t21l6i3f 98 25 3F 3F NNP cord-001257-t21l6i3f 98 26 , , , cord-001257-t21l6i3f 98 27 lanes lane VBZ cord-001257-t21l6i3f 98 28 6 6 CD cord-001257-t21l6i3f 98 29 - - SYM cord-001257-t21l6i3f 98 30 7 7 CD cord-001257-t21l6i3f 98 31 versus versus IN cord-001257-t21l6i3f 98 32 2 2 CD cord-001257-t21l6i3f 98 33 - - SYM cord-001257-t21l6i3f 98 34 3 3 CD cord-001257-t21l6i3f 98 35 ) ) -RRB- cord-001257-t21l6i3f 99 1 [ [ -LRB- cord-001257-t21l6i3f 99 2 57 57 CD cord-001257-t21l6i3f 99 3 ] ] -RRB- cord-001257-t21l6i3f 99 4 . . . cord-001257-t21l6i3f 100 1 Our -PRON- PRP$ cord-001257-t21l6i3f 100 2 data datum NNS cord-001257-t21l6i3f 100 3 also also RB cord-001257-t21l6i3f 100 4 support support VBP cord-001257-t21l6i3f 100 5 a a DT cord-001257-t21l6i3f 100 6 role role NN cord-001257-t21l6i3f 101 1 In in IN cord-001257-t21l6i3f 101 2 vitro vitro FW cord-001257-t21l6i3f 101 3 binding bind VBG cord-001257-t21l6i3f 101 4 assay assay NN cord-001257-t21l6i3f 101 5 with with IN cord-001257-t21l6i3f 101 6 purified purify VBN cord-001257-t21l6i3f 101 7 AtRH2 atrh2 NN cord-001257-t21l6i3f 101 8 . . . cord-001257-t21l6i3f 102 1 The the DT cord-001257-t21l6i3f 102 2 assay assay NN cord-001257-t21l6i3f 102 3 contained contain VBD cord-001257-t21l6i3f 102 4 the the DT cord-001257-t21l6i3f 102 5 32 32 CD cord-001257-t21l6i3f 102 6 P p NN cord-001257-t21l6i3f 102 7 - - HYPH cord-001257-t21l6i3f 102 8 labeled label VBN cord-001257-t21l6i3f 102 9 DI-72 DI-72 NNP cord-001257-t21l6i3f 102 10 ( ( -LRB- cord-001257-t21l6i3f 102 11 2)repRNA 2)repRNA NNP cord-001257-t21l6i3f 102 12 ( ( -LRB- cord-001257-t21l6i3f 102 13 , , , cord-001257-t21l6i3f 102 14 0.1 0.1 CD cord-001257-t21l6i3f 102 15 pmol pmol NN cord-001257-t21l6i3f 102 16 ) ) -RRB- cord-001257-t21l6i3f 102 17 plus plus CC cord-001257-t21l6i3f 102 18 increasing increase VBG cord-001257-t21l6i3f 102 19 amount amount NN cord-001257-t21l6i3f 102 20 of of IN cord-001257-t21l6i3f 102 21 unlabeled unlabeled JJ cord-001257-t21l6i3f 102 22 competitor competitor NN cord-001257-t21l6i3f 102 23 RNAs rna NNS cord-001257-t21l6i3f 102 24 , , , cord-001257-t21l6i3f 102 25 each each DT cord-001257-t21l6i3f 102 26 used use VBN cord-001257-t21l6i3f 102 27 in in IN cord-001257-t21l6i3f 102 28 the the DT cord-001257-t21l6i3f 102 29 same same JJ cord-001257-t21l6i3f 102 30 amounts amount NNS cord-001257-t21l6i3f 102 31 , , , cord-001257-t21l6i3f 102 32 including include VBG cord-001257-t21l6i3f 102 33 RI(2 RI(2 NNP cord-001257-t21l6i3f 102 34 ) ) -RRB- cord-001257-t21l6i3f 102 35 ( ( -LRB- cord-001257-t21l6i3f 102 36 3 3 CD cord-001257-t21l6i3f 102 37 and and CC cord-001257-t21l6i3f 102 38 6 6 CD cord-001257-t21l6i3f 102 39 pmol pmol NN cord-001257-t21l6i3f 102 40 ) ) -RRB- cord-001257-t21l6i3f 102 41 , , , cord-001257-t21l6i3f 102 42 RII(2 rii(2 NN cord-001257-t21l6i3f 102 43 ) ) -RRB- cord-001257-t21l6i3f 103 1 ( ( -LRB- cord-001257-t21l6i3f 103 2 2 2 CD cord-001257-t21l6i3f 103 3 and and CC cord-001257-t21l6i3f 103 4 4 4 CD cord-001257-t21l6i3f 103 5 pmol pmol NN cord-001257-t21l6i3f 103 6 ) ) -RRB- cord-001257-t21l6i3f 103 7 , , , cord-001257-t21l6i3f 103 8 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 103 9 ) ) -RRB- cord-001257-t21l6i3f 104 1 ( ( -LRB- cord-001257-t21l6i3f 104 2 5 5 CD cord-001257-t21l6i3f 104 3 and and CC cord-001257-t21l6i3f 104 4 10 10 CD cord-001257-t21l6i3f 104 5 pmol pmol NN cord-001257-t21l6i3f 104 6 ) ) -RRB- cord-001257-t21l6i3f 104 7 or or CC cord-001257-t21l6i3f 104 8 RIV(2 RIV(2 . cord-001257-t21l6i3f 104 9 ) ) -RRB- cord-001257-t21l6i3f 105 1 ( ( -LRB- cord-001257-t21l6i3f 105 2 4 4 CD cord-001257-t21l6i3f 105 3 and and CC cord-001257-t21l6i3f 105 4 8 8 CD cord-001257-t21l6i3f 105 5 pmol pmol NN cord-001257-t21l6i3f 105 6 ) ) -RRB- cord-001257-t21l6i3f 105 7 . . . cord-001257-t21l6i3f 106 1 The the DT cord-001257-t21l6i3f 106 2 free free JJ cord-001257-t21l6i3f 106 3 or or CC cord-001257-t21l6i3f 106 4 AtRH2-bound atrh2-bound NN cord-001257-t21l6i3f 106 5 ssRNA ssrna NN cord-001257-t21l6i3f 106 6 was be VBD cord-001257-t21l6i3f 106 7 separated separate VBN cord-001257-t21l6i3f 106 8 on on IN cord-001257-t21l6i3f 106 9 nondenaturing nondenature VBG cord-001257-t21l6i3f 106 10 5 5 CD cord-001257-t21l6i3f 106 11 % % NN cord-001257-t21l6i3f 106 12 acrylamide acrylamide NN cord-001257-t21l6i3f 106 13 gels gel NNS cord-001257-t21l6i3f 106 14 . . . cord-001257-t21l6i3f 107 1 ( ( -LRB- cord-001257-t21l6i3f 107 2 C C NNP cord-001257-t21l6i3f 107 3 ) ) -RRB- cord-001257-t21l6i3f 107 4 RNA RNA NNP cord-001257-t21l6i3f 107 5 gel gel NN cord-001257-t21l6i3f 107 6 shift shift NN cord-001257-t21l6i3f 107 7 analysis analysis NN cord-001257-t21l6i3f 107 8 shows show VBZ cord-001257-t21l6i3f 107 9 that that IN cord-001257-t21l6i3f 107 10 AtRH5 atrh5 NN cord-001257-t21l6i3f 107 11 binds bind VBZ cord-001257-t21l6i3f 107 12 the the DT cord-001257-t21l6i3f 107 13 most most RBS cord-001257-t21l6i3f 107 14 efficiently efficiently RB cord-001257-t21l6i3f 107 15 to to IN cord-001257-t21l6i3f 107 16 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 107 17 ) ) -RRB- cord-001257-t21l6i3f 107 18 . . . cord-001257-t21l6i3f 108 1 32 32 CD cord-001257-t21l6i3f 108 2 P p NN cord-001257-t21l6i3f 108 3 - - HYPH cord-001257-t21l6i3f 108 4 labeled label VBN cord-001257-t21l6i3f 108 5 DI-634 di-634 NN cord-001257-t21l6i3f 108 6 ( ( -LRB- cord-001257-t21l6i3f 108 7 2)repRNA 2)reprna CD cord-001257-t21l6i3f 108 8 template template NN cord-001257-t21l6i3f 108 9 ( ( -LRB- cord-001257-t21l6i3f 108 10 , , , cord-001257-t21l6i3f 108 11 0.1 0.1 CD cord-001257-t21l6i3f 108 12 pmol pmol NN cord-001257-t21l6i3f 108 13 ) ) -RRB- cord-001257-t21l6i3f 108 14 from from IN cord-001257-t21l6i3f 108 15 FHV FHV NNP cord-001257-t21l6i3f 108 16 and and CC cord-001257-t21l6i3f 108 17 unlabeled unlabeled JJ cord-001257-t21l6i3f 108 18 competitor competitor NN cord-001257-t21l6i3f 108 19 RNAs rna NNS cord-001257-t21l6i3f 108 20 ( ( -LRB- cord-001257-t21l6i3f 108 21 2 2 CD cord-001257-t21l6i3f 108 22 and and CC cord-001257-t21l6i3f 108 23 4 4 CD cord-001257-t21l6i3f 108 24 pmol pmol NN cord-001257-t21l6i3f 108 25 ) ) -RRB- cord-001257-t21l6i3f 108 26 representing represent VBG cord-001257-t21l6i3f 108 27 one one CD cord-001257-t21l6i3f 108 28 of of IN cord-001257-t21l6i3f 108 29 the the DT cord-001257-t21l6i3f 108 30 four four CD cord-001257-t21l6i3f 108 31 regions region NNS cord-001257-t21l6i3f 108 32 of of IN cord-001257-t21l6i3f 108 33 TBSV TBSV NNP cord-001257-t21l6i3f 108 34 DI-72 DI-72 NNP cord-001257-t21l6i3f 108 35 RNA RNA NNP cord-001257-t21l6i3f 108 36 from from IN cord-001257-t21l6i3f 108 37 both both DT cord-001257-t21l6i3f 108 38 RNA RNA NNP cord-001257-t21l6i3f 108 39 strands strand NNS cord-001257-t21l6i3f 108 40 ( ( -LRB- cord-001257-t21l6i3f 108 41 see see VB cord-001257-t21l6i3f 108 42 panel panel NNP cord-001257-t21l6i3f 108 43 A A NNP cord-001257-t21l6i3f 108 44 ) ) -RRB- cord-001257-t21l6i3f 108 45 were be VBD cord-001257-t21l6i3f 108 46 used use VBN cord-001257-t21l6i3f 108 47 in in IN cord-001257-t21l6i3f 108 48 the the DT cord-001257-t21l6i3f 108 49 competition competition NN cord-001257-t21l6i3f 108 50 assay assay NN cord-001257-t21l6i3f 108 51 . . . cord-001257-t21l6i3f 109 1 The the DT cord-001257-t21l6i3f 109 2 AtRH5 atrh5 NN cord-001257-t21l6i3f 109 3 -32 -32 NN cord-001257-t21l6i3f 109 4 P p NN cord-001257-t21l6i3f 109 5 - - HYPH cord-001257-t21l6i3f 109 6 labeled label VBN cord-001257-t21l6i3f 109 7 ssRNA ssrna NN cord-001257-t21l6i3f 109 8 complex complex NN cord-001257-t21l6i3f 109 9 was be VBD cord-001257-t21l6i3f 109 10 visualized visualize VBN cord-001257-t21l6i3f 109 11 on on IN cord-001257-t21l6i3f 109 12 nondenaturing nondenature VBG cord-001257-t21l6i3f 109 13 5 5 CD cord-001257-t21l6i3f 109 14 % % NN cord-001257-t21l6i3f 109 15 acrylamide acrylamide NN cord-001257-t21l6i3f 109 16 gels gel NNS cord-001257-t21l6i3f 109 17 . . . cord-001257-t21l6i3f 110 1 Each each DT cord-001257-t21l6i3f 110 2 experiment experiment NN cord-001257-t21l6i3f 110 3 was be VBD cord-001257-t21l6i3f 110 4 repeated repeat VBN cord-001257-t21l6i3f 110 5 at at IN cord-001257-t21l6i3f 110 6 least least RBS cord-001257-t21l6i3f 110 7 three three CD cord-001257-t21l6i3f 110 8 times time NNS cord-001257-t21l6i3f 110 9 . . . cord-001257-t21l6i3f 111 1 Note note VB cord-001257-t21l6i3f 111 2 that that IN cord-001257-t21l6i3f 111 3 we -PRON- PRP cord-001257-t21l6i3f 111 4 used use VBD cord-001257-t21l6i3f 111 5 the the DT cord-001257-t21l6i3f 111 6 heterologous heterologous JJ cord-001257-t21l6i3f 111 7 FHV FHV NNP cord-001257-t21l6i3f 112 1 DI-634 DI-634 NNP cord-001257-t21l6i3f 112 2 ( ( -LRB- cord-001257-t21l6i3f 112 3 2)RNA 2)rna CD cord-001257-t21l6i3f 112 4 in in IN cord-001257-t21l6i3f 112 5 the the DT cord-001257-t21l6i3f 112 6 binding binding JJ cord-001257-t21l6i3f 112 7 assay assay NN cord-001257-t21l6i3f 112 8 to to TO cord-001257-t21l6i3f 112 9 allow allow VB cord-001257-t21l6i3f 112 10 comparison comparison NN cord-001257-t21l6i3f 112 11 of of IN cord-001257-t21l6i3f 112 12 ( ( -LRB- cord-001257-t21l6i3f 112 13 + + NNS cord-001257-t21l6i3f 112 14 ) ) -RRB- cord-001257-t21l6i3f 112 15 versus versus IN cord-001257-t21l6i3f 112 16 ( ( -LRB- cord-001257-t21l6i3f 112 17 2)RNA 2)rna CD cord-001257-t21l6i3f 112 18 regions region NNS cord-001257-t21l6i3f 112 19 of of IN cord-001257-t21l6i3f 112 20 TBSV TBSV NNP cord-001257-t21l6i3f 112 21 RNA RNA NNP cord-001257-t21l6i3f 112 22 . . . cord-001257-t21l6i3f 113 1 The the DT cord-001257-t21l6i3f 113 2 template template NN cord-001257-t21l6i3f 113 3 competition competition NN cord-001257-t21l6i3f 113 4 assay assay NN cord-001257-t21l6i3f 113 5 showed show VBD cord-001257-t21l6i3f 113 6 efficient efficient JJ cord-001257-t21l6i3f 113 7 binding binding NN cord-001257-t21l6i3f 113 8 / / SYM cord-001257-t21l6i3f 113 9 competition competition NN cord-001257-t21l6i3f 113 10 by by IN cord-001257-t21l6i3f 113 11 the the DT cord-001257-t21l6i3f 113 12 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 113 13 ) ) -RRB- cord-001257-t21l6i3f 113 14 and and CC cord-001257-t21l6i3f 113 15 RI(+ RI(+ NNP cord-001257-t21l6i3f 113 16 ) ) -RRB- cord-001257-t21l6i3f 113 17 sequences sequence NNS cord-001257-t21l6i3f 113 18 for for IN cord-001257-t21l6i3f 113 19 AtRH5 atrh5 NN cord-001257-t21l6i3f 113 20 . . . cord-001257-t21l6i3f 114 1 ( ( -LRB- cord-001257-t21l6i3f 114 2 D d NN cord-001257-t21l6i3f 114 3 ) ) -RRB- cord-001257-t21l6i3f 115 1 Comparable comparable JJ cord-001257-t21l6i3f 115 2 viral viral JJ cord-001257-t21l6i3f 115 3 RNA RNA NNP cord-001257-t21l6i3f 115 4 binding binding NN cord-001257-t21l6i3f 115 5 assay assay NN cord-001257-t21l6i3f 115 6 reveals reveal VBZ cord-001257-t21l6i3f 115 7 different different JJ cord-001257-t21l6i3f 115 8 binding bind VBG cord-001257-t21l6i3f 115 9 specificity specificity NN cord-001257-t21l6i3f 115 10 for for IN cord-001257-t21l6i3f 115 11 DDX3-like DDX3-like NNP cord-001257-t21l6i3f 115 12 AtRH20 AtRH20 . cord-001257-t21l6i3f 116 1 and and CC cord-001257-t21l6i3f 116 2 the the DT cord-001257-t21l6i3f 116 3 DDX5-like ddx5-like XX cord-001257-t21l6i3f 117 1 AtRH5 atrh5 RB cord-001257-t21l6i3f 117 2 DEAD DEAD NNP cord-001257-t21l6i3f 117 3 - - HYPH cord-001257-t21l6i3f 117 4 box box NN cord-001257-t21l6i3f 117 5 helicases helicase NNS cord-001257-t21l6i3f 117 6 . . . cord-001257-t21l6i3f 118 1 See see VB cord-001257-t21l6i3f 118 2 additional additional JJ cord-001257-t21l6i3f 118 3 details detail NNS cord-001257-t21l6i3f 118 4 in in IN cord-001257-t21l6i3f 118 5 panel panel NN cord-001257-t21l6i3f 118 6 C. C. NNP cord-001257-t21l6i3f 119 1 The the DT cord-001257-t21l6i3f 119 2 template template NN cord-001257-t21l6i3f 119 3 competition competition NN cord-001257-t21l6i3f 119 4 assay assay NN cord-001257-t21l6i3f 119 5 showed show VBD cord-001257-t21l6i3f 119 6 efficient efficient JJ cord-001257-t21l6i3f 119 7 binding binding NN cord-001257-t21l6i3f 119 8 / / SYM cord-001257-t21l6i3f 119 9 competition competition NN cord-001257-t21l6i3f 119 10 by by IN cord-001257-t21l6i3f 119 11 the the DT cord-001257-t21l6i3f 119 12 RI(2 RI(2 NNP cord-001257-t21l6i3f 119 13 ) ) -RRB- cord-001257-t21l6i3f 119 14 , , , cord-001257-t21l6i3f 119 15 RII(2 RII(2 NNP cord-001257-t21l6i3f 119 16 ) ) -RRB- cord-001257-t21l6i3f 119 17 , , , cord-001257-t21l6i3f 119 18 RIV(2 RIV(2 NNP cord-001257-t21l6i3f 119 19 ) ) -RRB- cord-001257-t21l6i3f 119 20 and and CC cord-001257-t21l6i3f 119 21 RI(+ RI(+ NNP cord-001257-t21l6i3f 119 22 ) ) -RRB- cord-001257-t21l6i3f 119 23 sequences sequence NNS cord-001257-t21l6i3f 119 24 for for IN cord-001257-t21l6i3f 119 25 AtRH20 atrh20 NN cord-001257-t21l6i3f 119 26 . . . cord-001257-t21l6i3f 120 1 ( ( -LRB- cord-001257-t21l6i3f 120 2 E e NN cord-001257-t21l6i3f 120 3 ) ) -RRB- cord-001257-t21l6i3f 120 4 Schematic schematic JJ cord-001257-t21l6i3f 120 5 representation representation NN cord-001257-t21l6i3f 120 6 of of IN cord-001257-t21l6i3f 120 7 the the DT cord-001257-t21l6i3f 120 8 long long JJ cord-001257-t21l6i3f 120 9 - - HYPH cord-001257-t21l6i3f 120 10 range range NN cord-001257-t21l6i3f 120 11 RNA RNA NNP cord-001257-t21l6i3f 120 12 - - HYPH cord-001257-t21l6i3f 120 13 RNA RNA NNP cord-001257-t21l6i3f 120 14 interaction interaction NN cord-001257-t21l6i3f 120 15 between between IN cord-001257-t21l6i3f 120 16 the the DT cord-001257-t21l6i3f 120 17 ' ' `` cord-001257-t21l6i3f 120 18 ' ' `` cord-001257-t21l6i3f 120 19 base base NN cord-001257-t21l6i3f 120 20 ' ' '' cord-001257-t21l6i3f 120 21 ' ' '' cord-001257-t21l6i3f 120 22 sequence sequence NN cord-001257-t21l6i3f 120 23 in in IN cord-001257-t21l6i3f 120 24 the the DT cord-001257-t21l6i3f 120 25 cPR cpr NN cord-001257-t21l6i3f 120 26 promoter promoter NN cord-001257-t21l6i3f 120 27 in in IN cord-001257-t21l6i3f 120 28 RI(2 RI(2 NNP cord-001257-t21l6i3f 120 29 ) ) -RRB- cord-001257-t21l6i3f 120 30 and and CC cord-001257-t21l6i3f 120 31 the the DT cord-001257-t21l6i3f 120 32 complementary complementary JJ cord-001257-t21l6i3f 120 33 ' ' `` cord-001257-t21l6i3f 120 34 ' ' `` cord-001257-t21l6i3f 120 35 bridge bridge NN cord-001257-t21l6i3f 120 36 ' ' '' cord-001257-t21l6i3f 120 37 ' ' '' cord-001257-t21l6i3f 120 38 sequence sequence NN cord-001257-t21l6i3f 120 39 in in IN cord-001257-t21l6i3f 120 40 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 120 41 ) ) -RRB- cord-001257-t21l6i3f 121 1 REN REN NNP cord-001257-t21l6i3f 121 2 [ [ -LRB- cord-001257-t21l6i3f 121 3 56 56 CD cord-001257-t21l6i3f 121 4 ] ] -RRB- cord-001257-t21l6i3f 121 5 . . . cord-001257-t21l6i3f 122 1 The the DT cord-001257-t21l6i3f 122 2 competitor competitor NN cord-001257-t21l6i3f 122 3 RNAs rna NNS cord-001257-t21l6i3f 122 4 used use VBD cord-001257-t21l6i3f 122 5 in in IN cord-001257-t21l6i3f 122 6 panel panel NN cord-001257-t21l6i3f 122 7 F F NNP cord-001257-t21l6i3f 122 8 are be VBP cord-001257-t21l6i3f 122 9 shown show VBN cord-001257-t21l6i3f 122 10 schematically schematically RB cord-001257-t21l6i3f 122 11 . . . cord-001257-t21l6i3f 123 1 ( ( -LRB- cord-001257-t21l6i3f 123 2 F F NNP cord-001257-t21l6i3f 123 3 ) ) -RRB- cord-001257-t21l6i3f 124 1 The the DT cord-001257-t21l6i3f 124 2 bridge bridge NN cord-001257-t21l6i3f 124 3 sequence sequence NN cord-001257-t21l6i3f 124 4 contributes contribute VBZ cord-001257-t21l6i3f 124 5 to to IN cord-001257-t21l6i3f 124 6 binding bind VBG cord-001257-t21l6i3f 124 7 of of IN cord-001257-t21l6i3f 124 8 RIII RIII NNP cord-001257-t21l6i3f 124 9 ( ( -LRB- cord-001257-t21l6i3f 124 10 2 2 CD cord-001257-t21l6i3f 124 11 ) ) -RRB- cord-001257-t21l6i3f 124 12 to to IN cord-001257-t21l6i3f 124 13 AtRH5 atrh5 NN cord-001257-t21l6i3f 124 14 in in IN cord-001257-t21l6i3f 124 15 vitro vitro FW cord-001257-t21l6i3f 124 16 . . . cord-001257-t21l6i3f 125 1 Top top JJ cord-001257-t21l6i3f 125 2 image image NN cord-001257-t21l6i3f 125 3 : : : cord-001257-t21l6i3f 125 4 RNA rna NN cord-001257-t21l6i3f 125 5 - - HYPH cord-001257-t21l6i3f 125 6 binding bind VBG cord-001257-t21l6i3f 125 7 analysis analysis NN cord-001257-t21l6i3f 125 8 of of IN cord-001257-t21l6i3f 125 9 AtRH5 atrh5 NN cord-001257-t21l6i3f 125 10 -DI-72 -DI-72 . cord-001257-t21l6i3f 126 1 ( ( -LRB- cord-001257-t21l6i3f 126 2 2)repRNA 2)reprna CD cord-001257-t21l6i3f 126 3 interaction interaction NN cord-001257-t21l6i3f 126 4 after after IN cord-001257-t21l6i3f 126 5 UV UV NNP cord-001257-t21l6i3f 126 6 cross cross NN cord-001257-t21l6i3f 126 7 - - NN cord-001257-t21l6i3f 126 8 linking linking NN cord-001257-t21l6i3f 126 9 . . . cord-001257-t21l6i3f 127 1 32 32 CD cord-001257-t21l6i3f 127 2 for for IN cord-001257-t21l6i3f 127 3 the the DT cord-001257-t21l6i3f 127 4 bridge bridge NN cord-001257-t21l6i3f 127 5 sequence sequence NN cord-001257-t21l6i3f 127 6 in in IN cord-001257-t21l6i3f 127 7 binding bind VBG cord-001257-t21l6i3f 127 8 to to IN cord-001257-t21l6i3f 127 9 AtRH5 atrh5 NN cord-001257-t21l6i3f 128 1 ( ( -LRB- cord-001257-t21l6i3f 128 2 compare compare VB cord-001257-t21l6i3f 128 3 lanes lane NNS cord-001257-t21l6i3f 128 4 4 4 CD cord-001257-t21l6i3f 128 5 - - SYM cord-001257-t21l6i3f 128 6 5 5 CD cord-001257-t21l6i3f 128 7 with with IN cord-001257-t21l6i3f 128 8 2 2 CD cord-001257-t21l6i3f 128 9 - - SYM cord-001257-t21l6i3f 128 10 3 3 CD cord-001257-t21l6i3f 128 11 , , , cord-001257-t21l6i3f 128 12 Fig Fig NNP cord-001257-t21l6i3f 128 13 . . . cord-001257-t21l6i3f 128 14 3F 3F NNP cord-001257-t21l6i3f 128 15 ) ) -RRB- cord-001257-t21l6i3f 128 16 . . . cord-001257-t21l6i3f 129 1 The the DT cord-001257-t21l6i3f 129 2 efficient efficient JJ cord-001257-t21l6i3f 129 3 binding binding NN cord-001257-t21l6i3f 129 4 of of IN cord-001257-t21l6i3f 129 5 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 129 6 , , , cord-001257-t21l6i3f 129 7 AtRH5 atrh5 NN cord-001257-t21l6i3f 129 8 and and CC cord-001257-t21l6i3f 129 9 the the DT cord-001257-t21l6i3f 129 10 yeast yeast NN cord-001257-t21l6i3f 129 11 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 129 12 and and CC cord-001257-t21l6i3f 129 13 Fal1p Fal1p NNP cord-001257-t21l6i3f 129 14 to to IN cord-001257-t21l6i3f 129 15 the the DT cord-001257-t21l6i3f 129 16 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 129 17 ) ) -RRB- cord-001257-t21l6i3f 130 1 REN REN NNP cord-001257-t21l6i3f 130 2 region region NN cord-001257-t21l6i3f 130 3 indicates indicate VBZ cord-001257-t21l6i3f 130 4 that that IN cord-001257-t21l6i3f 130 5 these these DT cord-001257-t21l6i3f 130 6 helicases helicase NNS cord-001257-t21l6i3f 130 7 might may MD cord-001257-t21l6i3f 130 8 facilitate facilitate VB cord-001257-t21l6i3f 130 9 the the DT cord-001257-t21l6i3f 130 10 unwinding unwinding NN cord-001257-t21l6i3f 130 11 of of IN cord-001257-t21l6i3f 130 12 RNA RNA NNP cord-001257-t21l6i3f 130 13 structures structure NNS cord-001257-t21l6i3f 130 14 within within IN cord-001257-t21l6i3f 130 15 the the DT cord-001257-t21l6i3f 130 16 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 130 17 ) ) -RRB- cord-001257-t21l6i3f 130 18 REN REN NNP cord-001257-t21l6i3f 130 19 during during IN cord-001257-t21l6i3f 130 20 replication replication NN cord-001257-t21l6i3f 130 21 . . . cord-001257-t21l6i3f 131 1 Therefore therefore RB cord-001257-t21l6i3f 131 2 , , , cord-001257-t21l6i3f 131 3 we -PRON- PRP cord-001257-t21l6i3f 131 4 tested test VBD cord-001257-t21l6i3f 131 5 if if IN cord-001257-t21l6i3f 131 6 recombinant recombinant JJ cord-001257-t21l6i3f 131 7 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 131 8 and and CC cord-001257-t21l6i3f 131 9 AtRH5 atrh5 NN cord-001257-t21l6i3f 131 10 could could MD cord-001257-t21l6i3f 131 11 unwind unwind VB cord-001257-t21l6i3f 131 12 partial partial JJ cord-001257-t21l6i3f 131 13 RNA RNA NNP cord-001257-t21l6i3f 131 14 duplexes duplex NNS cord-001257-t21l6i3f 131 15 , , , cord-001257-t21l6i3f 131 16 which which WDT cord-001257-t21l6i3f 131 17 are be VBP cord-001257-t21l6i3f 131 18 known know VBN cord-001257-t21l6i3f 131 19 to to TO cord-001257-t21l6i3f 131 20 hinder hinder VB cord-001257-t21l6i3f 131 21 RdRp RdRp NNP cord-001257-t21l6i3f 131 22 - - HYPH cord-001257-t21l6i3f 131 23 driven drive VBN cord-001257-t21l6i3f 131 24 RNA RNA NNP cord-001257-t21l6i3f 131 25 synthesis synthesis NN cord-001257-t21l6i3f 131 26 [ [ -LRB- cord-001257-t21l6i3f 131 27 58 58 CD cord-001257-t21l6i3f 131 28 , , , cord-001257-t21l6i3f 131 29 59 59 CD cord-001257-t21l6i3f 131 30 ] ] -RRB- cord-001257-t21l6i3f 131 31 . . . cord-001257-t21l6i3f 132 1 We -PRON- PRP cord-001257-t21l6i3f 132 2 chose choose VBD cord-001257-t21l6i3f 132 3 partial partial JJ cord-001257-t21l6i3f 132 4 duplex duplex NN cord-001257-t21l6i3f 132 5 for for IN cord-001257-t21l6i3f 132 6 this this DT cord-001257-t21l6i3f 132 7 assay assay NN cord-001257-t21l6i3f 132 8 , , , cord-001257-t21l6i3f 132 9 because because IN cord-001257-t21l6i3f 132 10 DEAD DEAD NNP cord-001257-t21l6i3f 132 11 - - HYPH cord-001257-t21l6i3f 132 12 box box NN cord-001257-t21l6i3f 132 13 helicases helicase NNS cord-001257-t21l6i3f 132 14 are be VBP cord-001257-t21l6i3f 132 15 not not RB cord-001257-t21l6i3f 132 16 processive processive JJ cord-001257-t21l6i3f 132 17 enzymes enzyme NNS cord-001257-t21l6i3f 132 18 and and CC cord-001257-t21l6i3f 132 19 can can MD cord-001257-t21l6i3f 132 20 only only RB cord-001257-t21l6i3f 132 21 unwind unwind VB cord-001257-t21l6i3f 132 22 short short JJ cord-001257-t21l6i3f 132 23 duplexes duplex NNS cord-001257-t21l6i3f 132 24 [ [ -LRB- cord-001257-t21l6i3f 132 25 40 40 CD cord-001257-t21l6i3f 132 26 ] ] -RRB- cord-001257-t21l6i3f 132 27 . . . cord-001257-t21l6i3f 133 1 Interestingly interestingly RB cord-001257-t21l6i3f 133 2 , , , cord-001257-t21l6i3f 133 3 addition addition NN cord-001257-t21l6i3f 133 4 of of IN cord-001257-t21l6i3f 133 5 purified purify VBN cord-001257-t21l6i3f 133 6 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 133 7 and and CC cord-001257-t21l6i3f 133 8 AtRH5 atrh5 NN cord-001257-t21l6i3f 133 9 unwound unwind VBD cord-001257-t21l6i3f 133 10 the the DT cord-001257-t21l6i3f 133 11 partial partial JJ cord-001257-t21l6i3f 133 12 RNA RNA NNP cord-001257-t21l6i3f 133 13 duplex duplex NN cord-001257-t21l6i3f 134 1 ( ( -LRB- cord-001257-t21l6i3f 134 2 Fig Fig NNP cord-001257-t21l6i3f 134 3 . . . cord-001257-t21l6i3f 134 4 4B 4b CD cord-001257-t21l6i3f 134 5 , , , cord-001257-t21l6i3f 134 6 lanes lane NNS cord-001257-t21l6i3f 134 7 1 1 CD cord-001257-t21l6i3f 134 8 and and CC cord-001257-t21l6i3f 134 9 2 2 CD cord-001257-t21l6i3f 134 10 ) ) -RRB- cord-001257-t21l6i3f 134 11 and and CC cord-001257-t21l6i3f 135 1 the the DT cord-001257-t21l6i3f 135 2 yeast yeast NN cord-001257-t21l6i3f 135 3 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 135 4 and and CC cord-001257-t21l6i3f 135 5 Fal1p Fal1p NNP cord-001257-t21l6i3f 135 6 showed show VBD cord-001257-t21l6i3f 135 7 similar similar JJ cord-001257-t21l6i3f 135 8 activities activity NNS cord-001257-t21l6i3f 135 9 ( ( -LRB- cord-001257-t21l6i3f 135 10 lanes lane NNS cord-001257-t21l6i3f 135 11 7 7 CD cord-001257-t21l6i3f 135 12 - - SYM cord-001257-t21l6i3f 135 13 8 8 CD cord-001257-t21l6i3f 135 14 ) ) -RRB- cord-001257-t21l6i3f 135 15 . . . cord-001257-t21l6i3f 136 1 In in IN cord-001257-t21l6i3f 136 2 contrast contrast NN cord-001257-t21l6i3f 136 3 , , , cord-001257-t21l6i3f 136 4 Ded1p ded1p NN cord-001257-t21l6i3f 136 5 and and CC cord-001257-t21l6i3f 136 6 AtRH20 atrh20 NN cord-001257-t21l6i3f 136 7 helicases helicase NNS cord-001257-t21l6i3f 136 8 did do VBD cord-001257-t21l6i3f 136 9 not not RB cord-001257-t21l6i3f 136 10 efficiently efficiently RB cord-001257-t21l6i3f 136 11 unwind unwind VB cord-001257-t21l6i3f 136 12 the the DT cord-001257-t21l6i3f 136 13 RNA RNA NNP cord-001257-t21l6i3f 136 14 duplex duplex NN cord-001257-t21l6i3f 136 15 formed form VBN cord-001257-t21l6i3f 136 16 only only RB cord-001257-t21l6i3f 136 17 within within IN cord-001257-t21l6i3f 136 18 the the DT cord-001257-t21l6i3f 136 19 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 136 20 ) ) -RRB- cord-001257-t21l6i3f 136 21 REN REN NNP cord-001257-t21l6i3f 136 22 ( ( -LRB- cord-001257-t21l6i3f 136 23 Fig Fig NNP cord-001257-t21l6i3f 136 24 . . . cord-001257-t21l6i3f 136 25 4B 4b CD cord-001257-t21l6i3f 136 26 , , , cord-001257-t21l6i3f 136 27 lanes lane VBZ cord-001257-t21l6i3f 136 28 3 3 CD cord-001257-t21l6i3f 136 29 and and CC cord-001257-t21l6i3f 136 30 6 6 CD cord-001257-t21l6i3f 136 31 ) ) -RRB- cord-001257-t21l6i3f 136 32 . . . cord-001257-t21l6i3f 137 1 The the DT cord-001257-t21l6i3f 137 2 failure failure NN cord-001257-t21l6i3f 137 3 to to TO cord-001257-t21l6i3f 137 4 unwind unwind VB cord-001257-t21l6i3f 137 5 this this DT cord-001257-t21l6i3f 137 6 partial partial JJ cord-001257-t21l6i3f 137 7 duplex duplex NN cord-001257-t21l6i3f 137 8 by by IN cord-001257-t21l6i3f 137 9 Ded1p ded1p NN cord-001257-t21l6i3f 137 10 and and CC cord-001257-t21l6i3f 137 11 AtRH20 atrh20 NN cord-001257-t21l6i3f 137 12 is be VBZ cord-001257-t21l6i3f 137 13 likely likely JJ cord-001257-t21l6i3f 137 14 due due IN cord-001257-t21l6i3f 137 15 to to IN cord-001257-t21l6i3f 137 16 the the DT cord-001257-t21l6i3f 137 17 preference preference NN cord-001257-t21l6i3f 137 18 of of IN cord-001257-t21l6i3f 137 19 these these DT cord-001257-t21l6i3f 137 20 helicases helicase NNS cord-001257-t21l6i3f 137 21 to to TO cord-001257-t21l6i3f 137 22 bind bind VB cord-001257-t21l6i3f 137 23 the the DT cord-001257-t21l6i3f 137 24 RI(2 RI(2 NNP cord-001257-t21l6i3f 137 25 ) ) -RRB- cord-001257-t21l6i3f 137 26 sequence sequence NN cord-001257-t21l6i3f 137 27 of of IN cord-001257-t21l6i3f 137 28 the the DT cord-001257-t21l6i3f 137 29 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 137 30 RNA RNA NNP cord-001257-t21l6i3f 137 31 ( ( -LRB- cord-001257-t21l6i3f 137 32 Fig Fig NNP cord-001257-t21l6i3f 137 33 . . . cord-001257-t21l6i3f 137 34 3 3 CD cord-001257-t21l6i3f 137 35 ) ) -RRB- cord-001257-t21l6i3f 137 36 [ [ -LRB- cord-001257-t21l6i3f 137 37 30 30 CD cord-001257-t21l6i3f 137 38 , , , cord-001257-t21l6i3f 137 39 49 49 CD cord-001257-t21l6i3f 137 40 ] ] -RRB- cord-001257-t21l6i3f 137 41 . . . cord-001257-t21l6i3f 138 1 Overall overall RB cord-001257-t21l6i3f 138 2 , , , cord-001257-t21l6i3f 138 3 the the DT cord-001257-t21l6i3f 138 4 unwinding unwind VBG cord-001257-t21l6i3f 138 5 assay assay NN cord-001257-t21l6i3f 138 6 further further RB cord-001257-t21l6i3f 138 7 supported support VBD cord-001257-t21l6i3f 138 8 that that IN cord-001257-t21l6i3f 138 9 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 138 10 , , , cord-001257-t21l6i3f 138 11 AtRH5 atrh5 NN cord-001257-t21l6i3f 138 12 and and CC cord-001257-t21l6i3f 138 13 the the DT cord-001257-t21l6i3f 138 14 yeast yeast NN cord-001257-t21l6i3f 138 15 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 138 16 and and CC cord-001257-t21l6i3f 138 17 Fal1p Fal1p NNP cord-001257-t21l6i3f 138 18 have have VBP cord-001257-t21l6i3f 138 19 a a DT cord-001257-t21l6i3f 138 20 novel novel JJ cord-001257-t21l6i3f 138 21 pro pro JJ cord-001257-t21l6i3f 138 22 - - JJ cord-001257-t21l6i3f 138 23 viral viral JJ cord-001257-t21l6i3f 138 24 function function NN cord-001257-t21l6i3f 138 25 during during IN cord-001257-t21l6i3f 138 26 TBSV TBSV NNP cord-001257-t21l6i3f 138 27 replication replication NN cord-001257-t21l6i3f 138 28 that that WDT cord-001257-t21l6i3f 138 29 is be VBZ cord-001257-t21l6i3f 138 30 based base VBN cord-001257-t21l6i3f 138 31 on on IN cord-001257-t21l6i3f 138 32 interaction interaction NN cord-001257-t21l6i3f 138 33 with with IN cord-001257-t21l6i3f 138 34 the the DT cord-001257-t21l6i3f 138 35 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 138 36 ) ) -RRB- cord-001257-t21l6i3f 138 37 REN REN NNP cord-001257-t21l6i3f 138 38 element element NN cord-001257-t21l6i3f 138 39 . . . cord-001257-t21l6i3f 139 1 To to TO cord-001257-t21l6i3f 139 2 test test VB cord-001257-t21l6i3f 139 3 the the DT cord-001257-t21l6i3f 139 4 direct direct JJ cord-001257-t21l6i3f 139 5 effect effect NN cord-001257-t21l6i3f 139 6 of of IN cord-001257-t21l6i3f 139 7 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 139 8 and and CC cord-001257-t21l6i3f 139 9 AtRH5 atrh5 NN cord-001257-t21l6i3f 139 10 helicases helicase NNS cord-001257-t21l6i3f 139 11 on on IN cord-001257-t21l6i3f 139 12 TBSV TBSV NNP cord-001257-t21l6i3f 139 13 RNA RNA NNP cord-001257-t21l6i3f 139 14 synthesis synthesis NN cord-001257-t21l6i3f 139 15 , , , cord-001257-t21l6i3f 139 16 we -PRON- PRP cord-001257-t21l6i3f 139 17 utilized utilize VBD cord-001257-t21l6i3f 139 18 detergent detergent NN cord-001257-t21l6i3f 139 19 - - HYPH cord-001257-t21l6i3f 139 20 solubilized solubilize VBN cord-001257-t21l6i3f 139 21 and and CC cord-001257-t21l6i3f 139 22 affinity affinity NN cord-001257-t21l6i3f 139 23 - - HYPH cord-001257-t21l6i3f 139 24 purified purify VBN cord-001257-t21l6i3f 139 25 tombusvirus tombusvirus NN cord-001257-t21l6i3f 139 26 replicase replicase NN cord-001257-t21l6i3f 139 27 from from IN cord-001257-t21l6i3f 139 28 yeast yeast NN cord-001257-t21l6i3f 139 29 with with IN cord-001257-t21l6i3f 139 30 down down RB cord-001257-t21l6i3f 139 31 regulated regulate VBN cord-001257-t21l6i3f 139 32 Fal1p fal1p XX cord-001257-t21l6i3f 140 1 ( ( -LRB- cord-001257-t21l6i3f 140 2 Fig Fig NNP cord-001257-t21l6i3f 140 3 . . . cord-001257-t21l6i3f 140 4 5A 5a CD cord-001257-t21l6i3f 140 5 ) ) -RRB- cord-001257-t21l6i3f 140 6 . . . cord-001257-t21l6i3f 141 1 The the DT cord-001257-t21l6i3f 141 2 requirement requirement NN cord-001257-t21l6i3f 141 3 for for IN cord-001257-t21l6i3f 141 4 Fal1p Fal1p NNP cord-001257-t21l6i3f 141 5 helicase helicase NN cord-001257-t21l6i3f 141 6 on on IN cord-001257-t21l6i3f 141 7 RNA RNA NNP cord-001257-t21l6i3f 141 8 synthesis synthesis NN cord-001257-t21l6i3f 141 9 was be VBD cord-001257-t21l6i3f 141 10 supported support VBN cord-001257-t21l6i3f 141 11 by by IN cord-001257-t21l6i3f 141 12 the the DT cord-001257-t21l6i3f 141 13 observed observed JJ cord-001257-t21l6i3f 141 14 , , , cord-001257-t21l6i3f 141 15 55 55 CD cord-001257-t21l6i3f 141 16 % % NN cord-001257-t21l6i3f 141 17 decrease decrease NN cord-001257-t21l6i3f 141 18 of of IN cord-001257-t21l6i3f 141 19 the the DT cord-001257-t21l6i3f 141 20 in in FW cord-001257-t21l6i3f 141 21 vitro vitro FW cord-001257-t21l6i3f 141 22 activity activity NN cord-001257-t21l6i3f 141 23 of of IN cord-001257-t21l6i3f 141 24 the the DT cord-001257-t21l6i3f 141 25 purified purify VBN cord-001257-t21l6i3f 141 26 replicase replicase NN cord-001257-t21l6i3f 141 27 obtained obtain VBN cord-001257-t21l6i3f 141 28 from from IN cord-001257-t21l6i3f 141 29 yeast yeast NN cord-001257-t21l6i3f 141 30 with with IN cord-001257-t21l6i3f 141 31 depleted deplete VBN cord-001257-t21l6i3f 141 32 Fal1p fal1p NN cord-001257-t21l6i3f 141 33 when when WRB cord-001257-t21l6i3f 141 34 compared compare VBN cord-001257-t21l6i3f 141 35 with with IN cord-001257-t21l6i3f 141 36 the the DT cord-001257-t21l6i3f 141 37 replicase replicase NN cord-001257-t21l6i3f 141 38 from from IN cord-001257-t21l6i3f 141 39 yeast yeast NN cord-001257-t21l6i3f 141 40 expressing express VBG cord-001257-t21l6i3f 141 41 Fal1p fal1p XX cord-001257-t21l6i3f 141 42 at at IN cord-001257-t21l6i3f 141 43 high high JJ cord-001257-t21l6i3f 141 44 level level NN cord-001257-t21l6i3f 141 45 ( ( -LRB- cord-001257-t21l6i3f 141 46 Fig Fig NNP cord-001257-t21l6i3f 141 47 . . . cord-001257-t21l6i3f 141 48 5B 5b CD cord-001257-t21l6i3f 141 49 ) ) -RRB- cord-001257-t21l6i3f 141 50 . . . cord-001257-t21l6i3f 142 1 This this DT cord-001257-t21l6i3f 142 2 purified purify VBN cord-001257-t21l6i3f 142 3 replicase replicase NN cord-001257-t21l6i3f 142 4 can can MD cord-001257-t21l6i3f 142 5 only only RB cord-001257-t21l6i3f 142 6 synthesize synthesize VB cord-001257-t21l6i3f 142 7 complementary complementary JJ cord-001257-t21l6i3f 142 8 RNA RNA NNP cord-001257-t21l6i3f 142 9 products product NNS cord-001257-t21l6i3f 142 10 on on IN cord-001257-t21l6i3f 142 11 added add VBN cord-001257-t21l6i3f 142 12 TBSV TBSV NNP cord-001257-t21l6i3f 142 13 templates template NNS cord-001257-t21l6i3f 142 14 allowing allow VBG cord-001257-t21l6i3f 142 15 for for IN cord-001257-t21l6i3f 142 16 the the DT cord-001257-t21l6i3f 142 17 measurement measurement NN cord-001257-t21l6i3f 142 18 of of IN cord-001257-t21l6i3f 142 19 the the DT cord-001257-t21l6i3f 142 20 level level NN cord-001257-t21l6i3f 142 21 of of IN cord-001257-t21l6i3f 142 22 RNA RNA NNP cord-001257-t21l6i3f 142 23 synthesis synthesis NN cord-001257-t21l6i3f 142 24 [ [ -LRB- cord-001257-t21l6i3f 142 25 15 15 CD cord-001257-t21l6i3f 142 26 , , , cord-001257-t21l6i3f 142 27 16 16 CD cord-001257-t21l6i3f 142 28 ] ] -RRB- cord-001257-t21l6i3f 142 29 . . . cord-001257-t21l6i3f 143 1 We -PRON- PRP cord-001257-t21l6i3f 143 2 found find VBD cord-001257-t21l6i3f 143 3 that that IN cord-001257-t21l6i3f 143 4 addition addition NN cord-001257-t21l6i3f 143 5 of of IN cord-001257-t21l6i3f 143 6 purified purify VBN cord-001257-t21l6i3f 143 7 recombinant recombinant JJ cord-001257-t21l6i3f 143 8 AtRH2 atrh2 NN cord-001257-t21l6i3f 143 9 and and CC cord-001257-t21l6i3f 143 10 AtRH5 atrh5 NN cord-001257-t21l6i3f 143 11 helicases helicase NNS cord-001257-t21l6i3f 143 12 to to IN cord-001257-t21l6i3f 143 13 the the DT cord-001257-t21l6i3f 143 14 purified purify VBN cord-001257-t21l6i3f 143 15 tombusvirus tombusvirus NN cord-001257-t21l6i3f 143 16 replicase replicase NN cord-001257-t21l6i3f 143 17 ( ( -LRB- cord-001257-t21l6i3f 143 18 obtained obtain VBN cord-001257-t21l6i3f 143 19 from from IN cord-001257-t21l6i3f 143 20 Fal1p Fal1p NNP cord-001257-t21l6i3f 143 21 depleted deplete VBN cord-001257-t21l6i3f 143 22 yeast yeast NN cord-001257-t21l6i3f 143 23 ) ) -RRB- cord-001257-t21l6i3f 143 24 programmed program VBN cord-001257-t21l6i3f 143 25 with with IN cord-001257-t21l6i3f 143 26 the the DT cord-001257-t21l6i3f 143 27 DI-72 DI-72 NNP cord-001257-t21l6i3f 143 28 ( ( -LRB- cord-001257-t21l6i3f 143 29 2)repRNA 2)reprna CD cord-001257-t21l6i3f 143 30 stimulated stimulated JJ cord-001257-t21l6i3f 143 31 ( ( -LRB- cord-001257-t21l6i3f 143 32 + + NN cord-001257-t21l6i3f 143 33 ) ) -RRB- cord-001257-t21l6i3f 143 34 -strand -strand CD cord-001257-t21l6i3f 143 35 synthesis synthesis NN cord-001257-t21l6i3f 143 36 by by IN cord-001257-t21l6i3f 143 37 up up IN cord-001257-t21l6i3f 143 38 to to IN cord-001257-t21l6i3f 144 1 2-fold 2-fold LS cord-001257-t21l6i3f 144 2 ( ( -LRB- cord-001257-t21l6i3f 144 3 Fig Fig NNP cord-001257-t21l6i3f 144 4 . . . cord-001257-t21l6i3f 144 5 5C 5c CD cord-001257-t21l6i3f 144 6 , , , cord-001257-t21l6i3f 144 7 lanes lane NNS cord-001257-t21l6i3f 144 8 2 2 CD cord-001257-t21l6i3f 144 9 versus versus IN cord-001257-t21l6i3f 144 10 3 3 CD cord-001257-t21l6i3f 144 11 and and CC cord-001257-t21l6i3f 144 12 7 7 CD cord-001257-t21l6i3f 144 13 versus versus IN cord-001257-t21l6i3f 144 14 8) 8) NN cord-001257-t21l6i3f 144 15 . . . cord-001257-t21l6i3f 145 1 Interestingly interestingly RB cord-001257-t21l6i3f 145 2 , , , cord-001257-t21l6i3f 145 3 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 145 4 and and CC cord-001257-t21l6i3f 145 5 AtRH5 atrh5 NN cord-001257-t21l6i3f 145 6 helicases helicase NNS cord-001257-t21l6i3f 145 7 stimulated stimulate VBD cord-001257-t21l6i3f 145 8 the the DT cord-001257-t21l6i3f 145 9 production production NN cord-001257-t21l6i3f 145 10 of of IN cord-001257-t21l6i3f 145 11 both both CC cord-001257-t21l6i3f 145 12 full full JJ cord-001257-t21l6i3f 145 13 - - HYPH cord-001257-t21l6i3f 145 14 length length NN cord-001257-t21l6i3f 145 15 ( ( -LRB- cord-001257-t21l6i3f 145 16 + + NNS cord-001257-t21l6i3f 145 17 ) ) -RRB- cord-001257-t21l6i3f 145 18 -strand -strand CD cord-001257-t21l6i3f 145 19 repRNA repRNA NNS cord-001257-t21l6i3f 145 20 product product NN cord-001257-t21l6i3f 145 21 ( ( -LRB- cord-001257-t21l6i3f 145 22 via via IN cord-001257-t21l6i3f 145 23 de de FW cord-001257-t21l6i3f 145 24 novo novo NNP cord-001257-t21l6i3f 145 25 initiation initiation NNP cord-001257-t21l6i3f 145 26 ) ) -RRB- cord-001257-t21l6i3f 145 27 and and CC cord-001257-t21l6i3f 145 28 the the DT cord-001257-t21l6i3f 145 29 39-terminal 39-terminal CD cord-001257-t21l6i3f 145 30 extension extension NN cord-001257-t21l6i3f 145 31 product product NN cord-001257-t21l6i3f 145 32 ( ( -LRB- cord-001257-t21l6i3f 145 33 39TEX 39tex UH cord-001257-t21l6i3f 145 34 ; ; : cord-001257-t21l6i3f 145 35 due due IN cord-001257-t21l6i3f 145 36 to to IN cord-001257-t21l6i3f 145 37 initiation initiation NN cord-001257-t21l6i3f 145 38 of of IN cord-001257-t21l6i3f 145 39 complementary complementary JJ cord-001257-t21l6i3f 145 40 RNA RNA NNP cord-001257-t21l6i3f 145 41 synthesis synthesis NN cord-001257-t21l6i3f 145 42 by by IN cord-001257-t21l6i3f 145 43 selfpriming selfprime VBG cord-001257-t21l6i3f 145 44 from from IN cord-001257-t21l6i3f 145 45 the the DT cord-001257-t21l6i3f 145 46 39 39 CD cord-001257-t21l6i3f 145 47 end end NN cord-001257-t21l6i3f 145 48 of of IN cord-001257-t21l6i3f 145 49 the the DT cord-001257-t21l6i3f 145 50 template template NN cord-001257-t21l6i3f 145 51 , , , cord-001257-t21l6i3f 145 52 instead instead RB cord-001257-t21l6i3f 145 53 of of IN cord-001257-t21l6i3f 145 54 de de NNP cord-001257-t21l6i3f 145 55 novo novo NNP cord-001257-t21l6i3f 145 56 initiation initiation NNP cord-001257-t21l6i3f 145 57 [ [ -LRB- cord-001257-t21l6i3f 145 58 60 60 CD cord-001257-t21l6i3f 145 59 ] ] -RRB- cord-001257-t21l6i3f 145 60 [ [ -LRB- cord-001257-t21l6i3f 145 61 61 61 CD cord-001257-t21l6i3f 145 62 ] ] -RRB- cord-001257-t21l6i3f 145 63 [ [ -LRB- cord-001257-t21l6i3f 145 64 62 62 CD cord-001257-t21l6i3f 145 65 ] ] -RRB- cord-001257-t21l6i3f 145 66 ) ) -RRB- cord-001257-t21l6i3f 145 67 . . . cord-001257-t21l6i3f 146 1 This this DT cord-001257-t21l6i3f 146 2 is be VBZ cord-001257-t21l6i3f 146 3 in in IN cord-001257-t21l6i3f 146 4 contrast contrast NN cord-001257-t21l6i3f 146 5 with with IN cord-001257-t21l6i3f 146 6 AtRH20 atrh20 NN cord-001257-t21l6i3f 146 7 and and CC cord-001257-t21l6i3f 146 8 Ded1p ded1p IN cord-001257-t21l6i3f 146 9 helicases helicase NNS cord-001257-t21l6i3f 146 10 , , , cord-001257-t21l6i3f 146 11 which which WDT cord-001257-t21l6i3f 146 12 stimulated stimulate VBD cord-001257-t21l6i3f 146 13 the the DT cord-001257-t21l6i3f 146 14 production production NN cord-001257-t21l6i3f 146 15 of of IN cord-001257-t21l6i3f 146 16 mostly mostly RB cord-001257-t21l6i3f 146 17 full full JJ cord-001257-t21l6i3f 146 18 - - HYPH cord-001257-t21l6i3f 146 19 length length NN cord-001257-t21l6i3f 146 20 ( ( -LRB- cord-001257-t21l6i3f 146 21 + + CC cord-001257-t21l6i3f 147 1 ) ) -RRB- cord-001257-t21l6i3f 147 2 -strand -strand CD cord-001257-t21l6i3f 147 3 repRNA repRNA NNS cord-001257-t21l6i3f 147 4 product product NN cord-001257-t21l6i3f 148 1 ( ( -LRB- cord-001257-t21l6i3f 148 2 Fig Fig NNP cord-001257-t21l6i3f 148 3 . . . cord-001257-t21l6i3f 148 4 5C 5c CD cord-001257-t21l6i3f 148 5 , , , cord-001257-t21l6i3f 148 6 lanes lane NNS cord-001257-t21l6i3f 148 7 4 4 CD cord-001257-t21l6i3f 148 8 and and CC cord-001257-t21l6i3f 148 9 9 9 CD cord-001257-t21l6i3f 148 10 ) ) -RRB- cord-001257-t21l6i3f 149 1 [ [ -LRB- cord-001257-t21l6i3f 149 2 30 30 CD cord-001257-t21l6i3f 149 3 , , , cord-001257-t21l6i3f 149 4 49 49 CD cord-001257-t21l6i3f 149 5 ] ] -RRB- cord-001257-t21l6i3f 149 6 . . . cord-001257-t21l6i3f 150 1 Time time NN cord-001257-t21l6i3f 150 2 - - HYPH cord-001257-t21l6i3f 150 3 course course NN cord-001257-t21l6i3f 150 4 experiments experiment NNS cord-001257-t21l6i3f 150 5 with with IN cord-001257-t21l6i3f 150 6 the the DT cord-001257-t21l6i3f 150 7 purified purify VBN cord-001257-t21l6i3f 150 8 tombusvirus tombusvirus NN cord-001257-t21l6i3f 150 9 replicase replicase NN cord-001257-t21l6i3f 150 10 and and CC cord-001257-t21l6i3f 150 11 a a DT cord-001257-t21l6i3f 150 12 minimal minimal JJ cord-001257-t21l6i3f 150 13 template template NN cord-001257-t21l6i3f 150 14 carrying carry VBG cord-001257-t21l6i3f 150 15 RI(2 RI(2 NNP cord-001257-t21l6i3f 150 16 ) ) -RRB- cord-001257-t21l6i3f 150 17 and and CC cord-001257-t21l6i3f 151 1 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 151 2 ) ) -RRB- cord-001257-t21l6i3f 152 1 REN REN NNP cord-001257-t21l6i3f 152 2 sequences sequence NNS cord-001257-t21l6i3f 152 3 confirmed confirm VBD cord-001257-t21l6i3f 152 4 that that IN cord-001257-t21l6i3f 152 5 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 152 6 and and CC cord-001257-t21l6i3f 152 7 AtRH5 atrh5 NN cord-001257-t21l6i3f 152 8 helicases helicase NNS cord-001257-t21l6i3f 152 9 stimulated stimulate VBD cord-001257-t21l6i3f 152 10 the the DT cord-001257-t21l6i3f 152 11 production production NN cord-001257-t21l6i3f 152 12 of of IN cord-001257-t21l6i3f 152 13 both both CC cord-001257-t21l6i3f 152 14 full full JJ cord-001257-t21l6i3f 152 15 - - HYPH cord-001257-t21l6i3f 152 16 length length NN cord-001257-t21l6i3f 152 17 ( ( -LRB- cord-001257-t21l6i3f 152 18 + + CC cord-001257-t21l6i3f 153 1 ) ) -RRB- cord-001257-t21l6i3f 153 2 -strand -strand CD cord-001257-t21l6i3f 153 3 RNA RNA NNP cord-001257-t21l6i3f 153 4 and and CC cord-001257-t21l6i3f 153 5 39TEX 39tex CD cord-001257-t21l6i3f 153 6 products product NNS cord-001257-t21l6i3f 153 7 by by IN cord-001257-t21l6i3f 153 8 , , , cord-001257-t21l6i3f 153 9 two two CD cord-001257-t21l6i3f 153 10 - - RB cord-001257-t21l6i3f 153 11 fold fold NN cord-001257-t21l6i3f 153 12 at at IN cord-001257-t21l6i3f 153 13 both both CC cord-001257-t21l6i3f 153 14 early early JJ cord-001257-t21l6i3f 153 15 and and CC cord-001257-t21l6i3f 153 16 late late JJ cord-001257-t21l6i3f 153 17 time time NN cord-001257-t21l6i3f 153 18 points point NNS cord-001257-t21l6i3f 153 19 ( ( -LRB- cord-001257-t21l6i3f 153 20 Fig Fig NNP cord-001257-t21l6i3f 153 21 . . . cord-001257-t21l6i3f 153 22 5D 5D NNP cord-001257-t21l6i3f 153 23 ) ) -RRB- cord-001257-t21l6i3f 153 24 . . . cord-001257-t21l6i3f 154 1 Interestingly interestingly RB cord-001257-t21l6i3f 154 2 , , , cord-001257-t21l6i3f 154 3 the the DT cord-001257-t21l6i3f 154 4 stimulation stimulation NN cord-001257-t21l6i3f 154 5 of of IN cord-001257-t21l6i3f 154 6 RNA RNA NNP cord-001257-t21l6i3f 154 7 synthesis synthesis NN cord-001257-t21l6i3f 154 8 by by IN cord-001257-t21l6i3f 154 9 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 154 10 and and CC cord-001257-t21l6i3f 154 11 AtRH5 atrh5 NN cord-001257-t21l6i3f 154 12 helicases helicase NNS cord-001257-t21l6i3f 154 13 is be VBZ cord-001257-t21l6i3f 154 14 lost lose VBN cord-001257-t21l6i3f 154 15 when when WRB cord-001257-t21l6i3f 154 16 we -PRON- PRP cord-001257-t21l6i3f 154 17 used use VBD cord-001257-t21l6i3f 154 18 a a DT cord-001257-t21l6i3f 154 19 ( ( -LRB- cord-001257-t21l6i3f 154 20 2)repRNA 2)reprna CD cord-001257-t21l6i3f 154 21 lacking lack VBG cord-001257-t21l6i3f 154 22 59 59 CD cord-001257-t21l6i3f 154 23 sequences sequence NNS cord-001257-t21l6i3f 154 24 including include VBG cord-001257-t21l6i3f 154 25 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 154 26 ) ) -RRB- cord-001257-t21l6i3f 155 1 REN REN NNP cord-001257-t21l6i3f 155 2 region region NN cord-001257-t21l6i3f 155 3 ( ( -LRB- cord-001257-t21l6i3f 155 4 Fig Fig NNP cord-001257-t21l6i3f 155 5 . . . cord-001257-t21l6i3f 155 6 5E 5E NNP cord-001257-t21l6i3f 155 7 , , , cord-001257-t21l6i3f 155 8 lanes lane VBZ cord-001257-t21l6i3f 155 9 3 3 CD cord-001257-t21l6i3f 155 10 - - SYM cord-001257-t21l6i3f 155 11 4 4 CD cord-001257-t21l6i3f 155 12 versus versus IN cord-001257-t21l6i3f 155 13 1 1 CD cord-001257-t21l6i3f 155 14 ) ) -RRB- cord-001257-t21l6i3f 155 15 . . . cord-001257-t21l6i3f 156 1 This this DT cord-001257-t21l6i3f 156 2 is be VBZ cord-001257-t21l6i3f 156 3 in in IN cord-001257-t21l6i3f 156 4 contrast contrast NN cord-001257-t21l6i3f 156 5 with with IN cord-001257-t21l6i3f 156 6 AtRH20 AtRH20 NNP cord-001257-t21l6i3f 156 7 , , , cord-001257-t21l6i3f 156 8 which which WDT cord-001257-t21l6i3f 156 9 was be VBD cord-001257-t21l6i3f 156 10 able able JJ cord-001257-t21l6i3f 156 11 to to TO cord-001257-t21l6i3f 156 12 stimulate stimulate VB cord-001257-t21l6i3f 156 13 the the DT cord-001257-t21l6i3f 156 14 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 156 15 replicase replicase NN cord-001257-t21l6i3f 156 16 activity activity NN cord-001257-t21l6i3f 156 17 on on IN cord-001257-t21l6i3f 156 18 this this DT cord-001257-t21l6i3f 156 19 template template NN cord-001257-t21l6i3f 156 20 RNA RNA NNP cord-001257-t21l6i3f 157 1 ( ( -LRB- cord-001257-t21l6i3f 157 2 Fig Fig NNP cord-001257-t21l6i3f 157 3 . . . cord-001257-t21l6i3f 157 4 5E 5E NNP cord-001257-t21l6i3f 157 5 , , , cord-001257-t21l6i3f 157 6 lane lane NNP cord-001257-t21l6i3f 157 7 2 2 CD cord-001257-t21l6i3f 157 8 ) ) -RRB- cord-001257-t21l6i3f 157 9 . . . cord-001257-t21l6i3f 158 1 This this DT cord-001257-t21l6i3f 158 2 observation observation NN cord-001257-t21l6i3f 158 3 was be VBD cord-001257-t21l6i3f 158 4 confirmed confirm VBN cord-001257-t21l6i3f 158 5 in in IN cord-001257-t21l6i3f 158 6 in in IN cord-001257-t21l6i3f 158 7 vitro vitro FW cord-001257-t21l6i3f 158 8 timecourse timecourse NN cord-001257-t21l6i3f 158 9 experiments experiment NNS cord-001257-t21l6i3f 158 10 with with IN cord-001257-t21l6i3f 158 11 the the DT cord-001257-t21l6i3f 158 12 purified purify VBN cord-001257-t21l6i3f 158 13 tombusvirus tombusvirus NN cord-001257-t21l6i3f 158 14 replicase replicase NN cord-001257-t21l6i3f 158 15 and and CC cord-001257-t21l6i3f 158 16 a a DT cord-001257-t21l6i3f 158 17 template template NN cord-001257-t21l6i3f 158 18 lacking lack VBG cord-001257-t21l6i3f 158 19 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 158 20 ) ) -RRB- cord-001257-t21l6i3f 159 1 REN REN NNP cord-001257-t21l6i3f 159 2 by by IN cord-001257-t21l6i3f 159 3 showing show VBG cord-001257-t21l6i3f 159 4 the the DT cord-001257-t21l6i3f 159 5 absence absence NN cord-001257-t21l6i3f 159 6 of of IN cord-001257-t21l6i3f 159 7 stimulation stimulation NN cord-001257-t21l6i3f 159 8 of of IN cord-001257-t21l6i3f 159 9 RNA RNA NNP cord-001257-t21l6i3f 159 10 synthesis synthesis NN cord-001257-t21l6i3f 159 11 products product NNS cord-001257-t21l6i3f 159 12 by by IN cord-001257-t21l6i3f 159 13 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 159 14 and and CC cord-001257-t21l6i3f 159 15 AtRH5 atrh5 NN cord-001257-t21l6i3f 159 16 helicases helicase NNS cord-001257-t21l6i3f 159 17 at at IN cord-001257-t21l6i3f 159 18 both both CC cord-001257-t21l6i3f 159 19 early early JJ cord-001257-t21l6i3f 159 20 and and CC cord-001257-t21l6i3f 159 21 late late JJ cord-001257-t21l6i3f 159 22 time time NN cord-001257-t21l6i3f 159 23 points point NNS cord-001257-t21l6i3f 160 1 ( ( -LRB- cord-001257-t21l6i3f 160 2 Fig Fig NNP cord-001257-t21l6i3f 160 3 . . . cord-001257-t21l6i3f 160 4 5F 5F NNP cord-001257-t21l6i3f 160 5 ) ) -RRB- cord-001257-t21l6i3f 160 6 . . . cord-001257-t21l6i3f 161 1 Therefore therefore RB cord-001257-t21l6i3f 161 2 , , , cord-001257-t21l6i3f 161 3 we -PRON- PRP cord-001257-t21l6i3f 161 4 suggest suggest VBP cord-001257-t21l6i3f 161 5 that that IN cord-001257-t21l6i3f 161 6 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 161 7 and and CC cord-001257-t21l6i3f 161 8 AtRH5 atrh5 NN cord-001257-t21l6i3f 161 9 helicases helicase NNS cord-001257-t21l6i3f 161 10 depend depend VBP cord-001257-t21l6i3f 161 11 on on IN cord-001257-t21l6i3f 161 12 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 161 13 ) ) -RRB- cord-001257-t21l6i3f 162 1 REN REN NNP cord-001257-t21l6i3f 162 2 to to TO cord-001257-t21l6i3f 162 3 facilitate facilitate VB cord-001257-t21l6i3f 162 4 the the DT cord-001257-t21l6i3f 162 5 overall overall JJ cord-001257-t21l6i3f 162 6 efficiency efficiency NN cord-001257-t21l6i3f 162 7 of of IN cord-001257-t21l6i3f 162 8 template template NN cord-001257-t21l6i3f 162 9 use use NN cord-001257-t21l6i3f 162 10 and and CC cord-001257-t21l6i3f 162 11 RNA RNA NNP cord-001257-t21l6i3f 162 12 synthesis synthesis NN cord-001257-t21l6i3f 162 13 on on IN cord-001257-t21l6i3f 162 14 the the DT cord-001257-t21l6i3f 162 15 ( ( -LRB- cord-001257-t21l6i3f 162 16 2)RNA 2)rna CD cord-001257-t21l6i3f 162 17 template template NN cord-001257-t21l6i3f 162 18 by by IN cord-001257-t21l6i3f 162 19 the the DT cord-001257-t21l6i3f 162 20 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 162 21 replicase replicase NN cord-001257-t21l6i3f 162 22 . . . cord-001257-t21l6i3f 163 1 To to TO cord-001257-t21l6i3f 163 2 test test VB cord-001257-t21l6i3f 163 3 if if IN cord-001257-t21l6i3f 163 4 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 163 5 and and CC cord-001257-t21l6i3f 163 6 AtRH5 atrh5 NN cord-001257-t21l6i3f 163 7 helicases helicase NNS cord-001257-t21l6i3f 163 8 could could MD cord-001257-t21l6i3f 163 9 also also RB cord-001257-t21l6i3f 163 10 stimulate stimulate VB cord-001257-t21l6i3f 163 11 RNA RNA NNP cord-001257-t21l6i3f 163 12 synthesis synthesis NN cord-001257-t21l6i3f 163 13 by by IN cord-001257-t21l6i3f 163 14 the the DT cord-001257-t21l6i3f 163 15 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 163 16 replicase replicase NNP cord-001257-t21l6i3f 163 17 on on IN cord-001257-t21l6i3f 163 18 dsRNA dsrna NN cord-001257-t21l6i3f 163 19 templates template NNS cord-001257-t21l6i3f 163 20 , , , cord-001257-t21l6i3f 163 21 which which WDT cord-001257-t21l6i3f 163 22 are be VBP cord-001257-t21l6i3f 163 23 formed form VBN cord-001257-t21l6i3f 163 24 during during IN cord-001257-t21l6i3f 163 25 TBSV TBSV NNP cord-001257-t21l6i3f 163 26 replication replication NN cord-001257-t21l6i3f 164 1 ( ( -LRB- cord-001257-t21l6i3f 164 2 Kovalev Kovalev NNP cord-001257-t21l6i3f 164 3 et et NNP cord-001257-t21l6i3f 164 4 al al NNP cord-001257-t21l6i3f 164 5 , , , cord-001257-t21l6i3f 164 6 in in IN cord-001257-t21l6i3f 164 7 press press NN cord-001257-t21l6i3f 164 8 ) ) -RRB- cord-001257-t21l6i3f 164 9 , , , cord-001257-t21l6i3f 164 10 we -PRON- PRP cord-001257-t21l6i3f 164 11 used use VBD cord-001257-t21l6i3f 164 12 partial partial JJ cord-001257-t21l6i3f 164 13 dsRNA dsrna NN cord-001257-t21l6i3f 164 14 duplexes duplex NNS cord-001257-t21l6i3f 164 15 ( ( -LRB- cord-001257-t21l6i3f 164 16 Fig Fig NNP cord-001257-t21l6i3f 164 17 . . . cord-001257-t21l6i3f 164 18 6A 6a CD cord-001257-t21l6i3f 164 19 ) ) -RRB- cord-001257-t21l6i3f 164 20 . . . cord-001257-t21l6i3f 165 1 The the DT cord-001257-t21l6i3f 165 2 tombusvirus tombusvirus NN cord-001257-t21l6i3f 165 3 replicase replicase NN cord-001257-t21l6i3f 165 4 is be VBZ cord-001257-t21l6i3f 165 5 inefficient inefficient JJ cord-001257-t21l6i3f 165 6 utilizing utilize VBG cord-001257-t21l6i3f 165 7 these these DT cord-001257-t21l6i3f 165 8 dsRNA dsrna NN cord-001257-t21l6i3f 165 9 templates template NNS cord-001257-t21l6i3f 165 10 in in IN cord-001257-t21l6i3f 165 11 vitro vitro FW cord-001257-t21l6i3f 165 12 [ [ -LRB- cord-001257-t21l6i3f 165 13 30 30 CD cord-001257-t21l6i3f 165 14 , , , cord-001257-t21l6i3f 165 15 49 49 CD cord-001257-t21l6i3f 165 16 , , , cord-001257-t21l6i3f 165 17 59 59 CD cord-001257-t21l6i3f 165 18 ] ] -RRB- cord-001257-t21l6i3f 165 19 . . . cord-001257-t21l6i3f 166 1 The the DT cord-001257-t21l6i3f 166 2 addition addition NN cord-001257-t21l6i3f 166 3 of of IN cord-001257-t21l6i3f 166 4 recombinant recombinant JJ cord-001257-t21l6i3f 166 5 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 166 6 and and CC cord-001257-t21l6i3f 166 7 AtRH5 atrh5 NN cord-001257-t21l6i3f 166 8 helicases helicase NNS cord-001257-t21l6i3f 166 9 stimulated stimulate VBD cord-001257-t21l6i3f 166 10 RNA RNA NNP cord-001257-t21l6i3f 166 11 synthesis synthesis NN cord-001257-t21l6i3f 166 12 by by IN cord-001257-t21l6i3f 166 13 up up IN cord-001257-t21l6i3f 166 14 to to IN cord-001257-t21l6i3f 166 15 , , , cord-001257-t21l6i3f 166 16 2.5-fold 2.5-fold CD cord-001257-t21l6i3f 166 17 in in IN cord-001257-t21l6i3f 166 18 vitro vitro FW cord-001257-t21l6i3f 166 19 on on IN cord-001257-t21l6i3f 166 20 a a DT cord-001257-t21l6i3f 166 21 partial partial JJ cord-001257-t21l6i3f 166 22 dsRNA dsrna NN cord-001257-t21l6i3f 166 23 template template NN cord-001257-t21l6i3f 166 24 that that WDT cord-001257-t21l6i3f 166 25 had have VBD cord-001257-t21l6i3f 166 26 both both DT cord-001257-t21l6i3f 166 27 RI(2 RI(2 NNP cord-001257-t21l6i3f 166 28 ) ) -RRB- cord-001257-t21l6i3f 166 29 and and CC cord-001257-t21l6i3f 166 30 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 166 31 ) ) -RRB- cord-001257-t21l6i3f 166 32 REN REN NNP cord-001257-t21l6i3f 166 33 as as IN cord-001257-t21l6i3f 166 34 part part NN cord-001257-t21l6i3f 166 35 of of IN cord-001257-t21l6i3f 166 36 the the DT cord-001257-t21l6i3f 166 37 duplex duplex NN cord-001257-t21l6i3f 167 1 ( ( -LRB- cord-001257-t21l6i3f 167 2 Fig Fig NNP cord-001257-t21l6i3f 167 3 . . . cord-001257-t21l6i3f 167 4 6A 6a CD cord-001257-t21l6i3f 167 5 , , , cord-001257-t21l6i3f 167 6 construct construct VB cord-001257-t21l6i3f 167 7 DRII DRII NNP cord-001257-t21l6i3f 167 8 and and CC cord-001257-t21l6i3f 167 9 6B 6b CD cord-001257-t21l6i3f 167 10 , , , cord-001257-t21l6i3f 167 11 lanes lane NNS cord-001257-t21l6i3f 167 12 2 2 CD cord-001257-t21l6i3f 167 13 and and CC cord-001257-t21l6i3f 167 14 8 8 CD cord-001257-t21l6i3f 167 15 versus versus IN cord-001257-t21l6i3f 167 16 1 1 CD cord-001257-t21l6i3f 167 17 and and CC cord-001257-t21l6i3f 167 18 7 7 CD cord-001257-t21l6i3f 167 19 ) ) -RRB- cord-001257-t21l6i3f 167 20 . . . cord-001257-t21l6i3f 168 1 This this DT cord-001257-t21l6i3f 168 2 level level NN cord-001257-t21l6i3f 168 3 of of IN cord-001257-t21l6i3f 168 4 stimulation stimulation NN cord-001257-t21l6i3f 168 5 was be VBD cord-001257-t21l6i3f 168 6 comparable comparable JJ cord-001257-t21l6i3f 168 7 to to IN cord-001257-t21l6i3f 168 8 that that DT cord-001257-t21l6i3f 168 9 obtained obtain VBN cord-001257-t21l6i3f 168 10 with with IN cord-001257-t21l6i3f 168 11 AtRH20 atrh20 IN cord-001257-t21l6i3f 168 12 that that DT cord-001257-t21l6i3f 168 13 targets target VBZ cord-001257-t21l6i3f 168 14 the the DT cord-001257-t21l6i3f 168 15 RI(2 RI(2 NNP cord-001257-t21l6i3f 168 16 ) ) -RRB- cord-001257-t21l6i3f 168 17 sequence sequence NN cord-001257-t21l6i3f 168 18 ( ( -LRB- cord-001257-t21l6i3f 168 19 Fig Fig NNP cord-001257-t21l6i3f 168 20 . . . cord-001257-t21l6i3f 168 21 6B 6b CD cord-001257-t21l6i3f 168 22 , , , cord-001257-t21l6i3f 168 23 lanes lane VBZ cord-001257-t21l6i3f 168 24 3 3 CD cord-001257-t21l6i3f 168 25 and and CC cord-001257-t21l6i3f 168 26 9 9 CD cord-001257-t21l6i3f 168 27 ) ) -RRB- cord-001257-t21l6i3f 168 28 . . . cord-001257-t21l6i3f 169 1 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 169 2 and and CC cord-001257-t21l6i3f 169 3 AtRH5 atrh5 NN cord-001257-t21l6i3f 169 4 helicases helicase NNS cord-001257-t21l6i3f 169 5 also also RB cord-001257-t21l6i3f 169 6 stimulated stimulate VBD cord-001257-t21l6i3f 169 7 RNA RNA NNP cord-001257-t21l6i3f 169 8 synthesis synthesis NN cord-001257-t21l6i3f 169 9 on on IN cord-001257-t21l6i3f 169 10 a a DT cord-001257-t21l6i3f 169 11 complete complete JJ cord-001257-t21l6i3f 169 12 dsRNA dsrna NN cord-001257-t21l6i3f 169 13 template template NN cord-001257-t21l6i3f 169 14 ( ( -LRB- cord-001257-t21l6i3f 169 15 Fig fig NN cord-001257-t21l6i3f 169 16 . . . cord-001257-t21l6i3f 170 1 S3A S3A NNP cord-001257-t21l6i3f 170 2 and and CC cord-001257-t21l6i3f 170 3 S3B s3b NN cord-001257-t21l6i3f 170 4 , , , cord-001257-t21l6i3f 170 5 lanes lane NNS cord-001257-t21l6i3f 170 6 9 9 CD cord-001257-t21l6i3f 170 7 and and CC cord-001257-t21l6i3f 170 8 11 11 CD cord-001257-t21l6i3f 170 9 versus versus IN cord-001257-t21l6i3f 170 10 14 14 CD cord-001257-t21l6i3f 170 11 ) ) -RRB- cord-001257-t21l6i3f 170 12 , , , cord-001257-t21l6i3f 170 13 producing produce VBG cord-001257-t21l6i3f 170 14 mostly mostly RB cord-001257-t21l6i3f 170 15 ( ( -LRB- cord-001257-t21l6i3f 170 16 + + NFP cord-001257-t21l6i3f 170 17 ) ) -RRB- cord-001257-t21l6i3f 170 18 RNA RNA NNP cord-001257-t21l6i3f 170 19 products product NNS cord-001257-t21l6i3f 170 20 ( ( -LRB- cord-001257-t21l6i3f 170 21 Fig Fig NNP cord-001257-t21l6i3f 170 22 . . . cord-001257-t21l6i3f 170 23 S3C S3C NNS cord-001257-t21l6i3f 170 24 ) ) -RRB- cord-001257-t21l6i3f 170 25 . . . cord-001257-t21l6i3f 171 1 Importantly importantly RB cord-001257-t21l6i3f 171 2 , , , cord-001257-t21l6i3f 171 3 the the DT cord-001257-t21l6i3f 171 4 stimulation stimulation NN cord-001257-t21l6i3f 171 5 of of IN cord-001257-t21l6i3f 171 6 RNA RNA NNP cord-001257-t21l6i3f 171 7 synthesis synthesis NN cord-001257-t21l6i3f 171 8 by by IN cord-001257-t21l6i3f 171 9 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 171 10 and and CC cord-001257-t21l6i3f 171 11 AtRH5 atrh5 NN cord-001257-t21l6i3f 171 12 helicases helicase NNS cord-001257-t21l6i3f 171 13 was be VBD cord-001257-t21l6i3f 171 14 lost lose VBN cord-001257-t21l6i3f 171 15 when when WRB cord-001257-t21l6i3f 171 16 a a DT cord-001257-t21l6i3f 171 17 partial partial JJ cord-001257-t21l6i3f 171 18 dsRNA dsrna NN cord-001257-t21l6i3f 171 19 template template NN cord-001257-t21l6i3f 171 20 lacking lack VBG cord-001257-t21l6i3f 171 21 the the DT cord-001257-t21l6i3f 171 22 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 171 23 ) ) -RRB- cord-001257-t21l6i3f 171 24 REN REN NNP cord-001257-t21l6i3f 171 25 was be VBD cord-001257-t21l6i3f 171 26 used use VBN cord-001257-t21l6i3f 171 27 ( ( -LRB- cord-001257-t21l6i3f 171 28 Fig Fig NNP cord-001257-t21l6i3f 171 29 . . . cord-001257-t21l6i3f 171 30 6A 6a CD cord-001257-t21l6i3f 171 31 , , , cord-001257-t21l6i3f 171 32 construct construct VB cord-001257-t21l6i3f 171 33 DRIII DRIII NNP cord-001257-t21l6i3f 171 34 and and CC cord-001257-t21l6i3f 171 35 6B 6b CD cord-001257-t21l6i3f 171 36 , , , cord-001257-t21l6i3f 171 37 lanes lane NNS cord-001257-t21l6i3f 171 38 5 5 CD cord-001257-t21l6i3f 171 39 and and CC cord-001257-t21l6i3f 171 40 11 11 CD cord-001257-t21l6i3f 171 41 versus versus IN cord-001257-t21l6i3f 171 42 4 4 CD cord-001257-t21l6i3f 171 43 and and CC cord-001257-t21l6i3f 171 44 10 10 CD cord-001257-t21l6i3f 171 45 ) ) -RRB- cord-001257-t21l6i3f 171 46 . . . cord-001257-t21l6i3f 172 1 In in IN cord-001257-t21l6i3f 172 2 contrast contrast NN cord-001257-t21l6i3f 172 3 , , , cord-001257-t21l6i3f 172 4 AtRH20 AtRH20 NNP cord-001257-t21l6i3f 172 5 was be VBD cord-001257-t21l6i3f 172 6 still still RB cord-001257-t21l6i3f 172 7 able able JJ cord-001257-t21l6i3f 172 8 to to TO cord-001257-t21l6i3f 172 9 stimulate stimulate VB cord-001257-t21l6i3f 172 10 RNA RNA NNP cord-001257-t21l6i3f 172 11 synthesis synthesis NN cord-001257-t21l6i3f 172 12 on on IN cord-001257-t21l6i3f 172 13 this this DT cord-001257-t21l6i3f 172 14 template template NN cord-001257-t21l6i3f 172 15 ( ( -LRB- cord-001257-t21l6i3f 172 16 Fig Fig NNP cord-001257-t21l6i3f 172 17 . . . cord-001257-t21l6i3f 172 18 6B 6b CD cord-001257-t21l6i3f 172 19 , , , cord-001257-t21l6i3f 172 20 lanes lane NNS cord-001257-t21l6i3f 172 21 6 6 CD cord-001257-t21l6i3f 172 22 and and CC cord-001257-t21l6i3f 172 23 12 12 CD cord-001257-t21l6i3f 172 24 ) ) -RRB- cord-001257-t21l6i3f 172 25 , , , cord-001257-t21l6i3f 172 26 as as IN cord-001257-t21l6i3f 172 27 predicted predict VBN cord-001257-t21l6i3f 172 28 based base VBN cord-001257-t21l6i3f 172 29 on on IN cord-001257-t21l6i3f 172 30 the the DT cord-001257-t21l6i3f 172 31 ability ability NN cord-001257-t21l6i3f 172 32 of of IN cord-001257-t21l6i3f 172 33 AtRH20 atrh20 NN cord-001257-t21l6i3f 172 34 to to TO cord-001257-t21l6i3f 172 35 bind bind VB cord-001257-t21l6i3f 172 36 to to IN cord-001257-t21l6i3f 172 37 RI(2 RI(2 NNP cord-001257-t21l6i3f 172 38 ) ) -RRB- cord-001257-t21l6i3f 172 39 sequence sequence NN cord-001257-t21l6i3f 172 40 [ [ -LRB- cord-001257-t21l6i3f 172 41 30 30 CD cord-001257-t21l6i3f 172 42 , , , cord-001257-t21l6i3f 172 43 49 49 CD cord-001257-t21l6i3f 172 44 ] ] -RRB- cord-001257-t21l6i3f 172 45 . . . cord-001257-t21l6i3f 173 1 Based base VBN cord-001257-t21l6i3f 173 2 on on IN cord-001257-t21l6i3f 173 3 these these DT cord-001257-t21l6i3f 173 4 data datum NNS cord-001257-t21l6i3f 173 5 , , , cord-001257-t21l6i3f 173 6 we -PRON- PRP cord-001257-t21l6i3f 173 7 conclude conclude VBP cord-001257-t21l6i3f 173 8 that that IN cord-001257-t21l6i3f 173 9 AtRH2 atrh2 NN cord-001257-t21l6i3f 174 1 To to TO cord-001257-t21l6i3f 174 2 further further RB cord-001257-t21l6i3f 174 3 study study VB cord-001257-t21l6i3f 174 4 the the DT cord-001257-t21l6i3f 174 5 roles role NNS cord-001257-t21l6i3f 174 6 of of IN cord-001257-t21l6i3f 174 7 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 174 8 and and CC cord-001257-t21l6i3f 174 9 AtRH5 atrh5 NN cord-001257-t21l6i3f 174 10 helicases helicase NNS cord-001257-t21l6i3f 174 11 in in IN cord-001257-t21l6i3f 174 12 TBSV TBSV NNP cord-001257-t21l6i3f 174 13 replication replication NN cord-001257-t21l6i3f 174 14 , , , cord-001257-t21l6i3f 174 15 we -PRON- PRP cord-001257-t21l6i3f 174 16 used use VBD cord-001257-t21l6i3f 174 17 whole whole JJ cord-001257-t21l6i3f 174 18 cell cell NN cord-001257-t21l6i3f 174 19 extracts extract NNS cord-001257-t21l6i3f 174 20 ( ( -LRB- cord-001257-t21l6i3f 174 21 CFE CFE NNP cord-001257-t21l6i3f 174 22 ) ) -RRB- cord-001257-t21l6i3f 174 23 prepared prepare VBD cord-001257-t21l6i3f 174 24 from from IN cord-001257-t21l6i3f 174 25 yeast yeast NN cord-001257-t21l6i3f 174 26 containing contain VBG cord-001257-t21l6i3f 174 27 temperature temperature NN cord-001257-t21l6i3f 174 28 - - HYPH cord-001257-t21l6i3f 174 29 sensitive sensitive JJ cord-001257-t21l6i3f 174 30 ( ( -LRB- cord-001257-t21l6i3f 174 31 ts ts NN cord-001257-t21l6i3f 174 32 ) ) -RRB- cord-001257-t21l6i3f 174 33 Fal1p Fal1p NNP cord-001257-t21l6i3f 174 34 and and CC cord-001257-t21l6i3f 174 35 lacking lack VBG cord-001257-t21l6i3f 174 36 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 174 37 to to TO cord-001257-t21l6i3f 174 38 support support VB cord-001257-t21l6i3f 174 39 cell cell NN cord-001257-t21l6i3f 174 40 - - HYPH cord-001257-t21l6i3f 174 41 free free JJ cord-001257-t21l6i3f 174 42 TBSV TBSV NNP cord-001257-t21l6i3f 174 43 replication replication NN cord-001257-t21l6i3f 174 44 . . . cord-001257-t21l6i3f 175 1 TBSV TBSV NNP cord-001257-t21l6i3f 175 2 ( ( -LRB- cord-001257-t21l6i3f 175 3 + + CD cord-001257-t21l6i3f 175 4 ) ) -RRB- cord-001257-t21l6i3f 176 1 RNA RNA NNP cord-001257-t21l6i3f 176 2 has have VBZ cord-001257-t21l6i3f 176 3 been be VBN cord-001257-t21l6i3f 176 4 shown show VBN cord-001257-t21l6i3f 176 5 to to TO cord-001257-t21l6i3f 176 6 perform perform VB cord-001257-t21l6i3f 176 7 one one CD cord-001257-t21l6i3f 176 8 full full JJ cord-001257-t21l6i3f 176 9 cycle cycle NN cord-001257-t21l6i3f 176 10 of of IN cord-001257-t21l6i3f 176 11 replication replication NN cord-001257-t21l6i3f 176 12 , , , cord-001257-t21l6i3f 176 13 starting start VBG cord-001257-t21l6i3f 176 14 with with IN cord-001257-t21l6i3f 176 15 VRC VRC NNP cord-001257-t21l6i3f 176 16 assembly assembly NN cord-001257-t21l6i3f 176 17 , , , cord-001257-t21l6i3f 176 18 ( ( -LRB- cord-001257-t21l6i3f 176 19 2)RNA 2)rna CD cord-001257-t21l6i3f 176 20 synthesis synthesis NN cord-001257-t21l6i3f 176 21 and and CC cord-001257-t21l6i3f 176 22 finally finally RB cord-001257-t21l6i3f 176 23 production production NN cord-001257-t21l6i3f 176 24 of of IN cord-001257-t21l6i3f 176 25 excess excess JJ cord-001257-t21l6i3f 176 26 amount amount NN cord-001257-t21l6i3f 176 27 of of IN cord-001257-t21l6i3f 176 28 ( ( -LRB- cord-001257-t21l6i3f 176 29 + + NNS cord-001257-t21l6i3f 176 30 ) ) -RRB- cord-001257-t21l6i3f 176 31 -strands -strand NNS cord-001257-t21l6i3f 176 32 , , , cord-001257-t21l6i3f 176 33 in in IN cord-001257-t21l6i3f 176 34 the the DT cord-001257-t21l6i3f 176 35 CFE CFE NNP cord-001257-t21l6i3f 176 36 - - HYPH cord-001257-t21l6i3f 176 37 based base VBN cord-001257-t21l6i3f 176 38 replication replication NN cord-001257-t21l6i3f 176 39 assay assay NN cord-001257-t21l6i3f 176 40 when when WRB cord-001257-t21l6i3f 176 41 purified purify VBN cord-001257-t21l6i3f 176 42 recombinant recombinant JJ cord-001257-t21l6i3f 176 43 p33 p33 NN cord-001257-t21l6i3f 176 44 and and CC cord-001257-t21l6i3f 176 45 p92 p92 NN cord-001257-t21l6i3f 176 46 pol pol NN cord-001257-t21l6i3f 176 47 replication replication NN cord-001257-t21l6i3f 176 48 proteins protein NNS cord-001257-t21l6i3f 176 49 are be VBP cord-001257-t21l6i3f 176 50 included include VBN cord-001257-t21l6i3f 176 51 [ [ -LRB- cord-001257-t21l6i3f 176 52 24 24 CD cord-001257-t21l6i3f 176 53 , , , cord-001257-t21l6i3f 176 54 63 63 CD cord-001257-t21l6i3f 176 55 ] ] -RRB- cord-001257-t21l6i3f 176 56 . . . cord-001257-t21l6i3f 177 1 The the DT cord-001257-t21l6i3f 177 2 CFE CFE NNP cord-001257-t21l6i3f 177 3 - - HYPH cord-001257-t21l6i3f 177 4 based base VBN cord-001257-t21l6i3f 177 5 replication replication NN cord-001257-t21l6i3f 177 6 assay assay NN cord-001257-t21l6i3f 177 7 showed show VBD cord-001257-t21l6i3f 177 8 that that IN cord-001257-t21l6i3f 177 9 ( ( -LRB- cord-001257-t21l6i3f 177 10 + + JJ cord-001257-t21l6i3f 177 11 ) ) -RRB- cord-001257-t21l6i3f 177 12 RNA rna NN cord-001257-t21l6i3f 177 13 synthesis synthesis NN cord-001257-t21l6i3f 177 14 decreased decrease VBD cord-001257-t21l6i3f 177 15 by by IN cord-001257-t21l6i3f 177 16 , , , cord-001257-t21l6i3f 177 17 2-fold 2-fold CD cord-001257-t21l6i3f 177 18 when when WRB cord-001257-t21l6i3f 177 19 compared compare VBN cord-001257-t21l6i3f 177 20 with with IN cord-001257-t21l6i3f 177 21 the the DT cord-001257-t21l6i3f 177 22 control control NN cord-001257-t21l6i3f 177 23 CFE CFE NNP cord-001257-t21l6i3f 177 24 prepared prepare VBD cord-001257-t21l6i3f 177 25 from from IN cord-001257-t21l6i3f 177 26 yeast yeast NN cord-001257-t21l6i3f 177 27 with with IN cord-001257-t21l6i3f 177 28 high high JJ cord-001257-t21l6i3f 177 29 level level NN cord-001257-t21l6i3f 177 30 of of IN cord-001257-t21l6i3f 177 31 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 178 1 and and CC cord-001257-t21l6i3f 178 2 wt wt NN cord-001257-t21l6i3f 178 3 Fal1p Fal1p NNP cord-001257-t21l6i3f 178 4 ( ( -LRB- cord-001257-t21l6i3f 179 1 Fig Fig NNP cord-001257-t21l6i3f 179 2 . . . cord-001257-t21l6i3f 180 1 S4B S4B NNP cord-001257-t21l6i3f 180 2 , , , cord-001257-t21l6i3f 180 3 lane lane NN cord-001257-t21l6i3f 180 4 4 4 CD cord-001257-t21l6i3f 180 5 versus versus IN cord-001257-t21l6i3f 180 6 1 1 CD cord-001257-t21l6i3f 180 7 ) ) -RRB- cord-001257-t21l6i3f 180 8 . . . cord-001257-t21l6i3f 181 1 This this DT cord-001257-t21l6i3f 181 2 is be VBZ cord-001257-t21l6i3f 181 3 in in IN cord-001257-t21l6i3f 181 4 contrast contrast NN cord-001257-t21l6i3f 181 5 with with IN cord-001257-t21l6i3f 181 6 ( ( -LRB- cord-001257-t21l6i3f 181 7 2)RNA 2)rna CD cord-001257-t21l6i3f 181 8 synthesis synthesis NN cord-001257-t21l6i3f 181 9 ( ( -LRB- cord-001257-t21l6i3f 181 10 represented represent VBN cord-001257-t21l6i3f 181 11 by by IN cord-001257-t21l6i3f 181 12 dsRNA dsrna NN cord-001257-t21l6i3f 181 13 product product NN cord-001257-t21l6i3f 181 14 ) ) -RRB- cord-001257-t21l6i3f 181 15 , , , cord-001257-t21l6i3f 181 16 which which WDT cord-001257-t21l6i3f 181 17 was be VBD cord-001257-t21l6i3f 181 18 unchanged unchanged JJ cord-001257-t21l6i3f 181 19 when when WRB cord-001257-t21l6i3f 181 20 the the DT cord-001257-t21l6i3f 181 21 CFE CFE NNP cord-001257-t21l6i3f 181 22 was be VBD cord-001257-t21l6i3f 181 23 prepared prepare VBN cord-001257-t21l6i3f 181 24 from from IN cord-001257-t21l6i3f 181 25 dbp3D dbp3D NNP cord-001257-t21l6i3f 181 26 / / SYM cord-001257-t21l6i3f 181 27 ts ts NN cord-001257-t21l6i3f 181 28 - - HYPH cord-001257-t21l6i3f 181 29 fal1 fal1 JJ cord-001257-t21l6i3f 181 30 yeast yeast NN cord-001257-t21l6i3f 181 31 versus versus IN cord-001257-t21l6i3f 181 32 wt wt NN cord-001257-t21l6i3f 181 33 yeast yeast NN cord-001257-t21l6i3f 181 34 . . . cord-001257-t21l6i3f 182 1 However however RB cord-001257-t21l6i3f 182 2 , , , cord-001257-t21l6i3f 182 3 addition addition NN cord-001257-t21l6i3f 182 4 of of IN cord-001257-t21l6i3f 182 5 purified purify VBN cord-001257-t21l6i3f 182 6 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 182 7 or or CC cord-001257-t21l6i3f 182 8 AtRH5 atrh5 NN cord-001257-t21l6i3f 182 9 to to IN cord-001257-t21l6i3f 182 10 the the DT cord-001257-t21l6i3f 182 11 CFE CFE NNP cord-001257-t21l6i3f 182 12 assay assay NN cord-001257-t21l6i3f 182 13 increased increase VBN cord-001257-t21l6i3f 182 14 ( ( -LRB- cord-001257-t21l6i3f 182 15 + + CC cord-001257-t21l6i3f 182 16 ) ) -RRB- cord-001257-t21l6i3f 182 17 RNA rna NN cord-001257-t21l6i3f 182 18 production production NN cord-001257-t21l6i3f 182 19 by by IN cord-001257-t21l6i3f 182 20 , , , cord-001257-t21l6i3f 182 21 60 60 CD cord-001257-t21l6i3f 182 22 - - SYM cord-001257-t21l6i3f 182 23 70 70 CD cord-001257-t21l6i3f 182 24 % % NN cord-001257-t21l6i3f 182 25 , , , cord-001257-t21l6i3f 182 26 while while IN cord-001257-t21l6i3f 182 27 the the DT cord-001257-t21l6i3f 182 28 ( ( -LRB- cord-001257-t21l6i3f 182 29 2 2 CD cord-001257-t21l6i3f 182 30 ) ) -RRB- cord-001257-t21l6i3f 182 31 RNA RNA NNP cord-001257-t21l6i3f 182 32 ( ( -LRB- cord-001257-t21l6i3f 182 33 in in IN cord-001257-t21l6i3f 182 34 the the DT cord-001257-t21l6i3f 182 35 form form NN cord-001257-t21l6i3f 182 36 of of IN cord-001257-t21l6i3f 182 37 dsRNA dsrna NN cord-001257-t21l6i3f 182 38 ) ) -RRB- cord-001257-t21l6i3f 182 39 was be VBD cord-001257-t21l6i3f 182 40 unchanged unchanged JJ cord-001257-t21l6i3f 183 1 ( ( -LRB- cord-001257-t21l6i3f 183 2 Fig Fig NNP cord-001257-t21l6i3f 183 3 . . . cord-001257-t21l6i3f 183 4 S4C s4c JJ cord-001257-t21l6i3f 183 5 - - HYPH cord-001257-t21l6i3f 183 6 D d JJ cord-001257-t21l6i3f 183 7 ) ) -RRB- cord-001257-t21l6i3f 183 8 . . . cord-001257-t21l6i3f 184 1 Thus thus RB cord-001257-t21l6i3f 184 2 , , , cord-001257-t21l6i3f 184 3 these these DT cord-001257-t21l6i3f 184 4 data datum NNS cord-001257-t21l6i3f 184 5 with with IN cord-001257-t21l6i3f 184 6 CFE CFE NNP cord-001257-t21l6i3f 184 7 - - HYPH cord-001257-t21l6i3f 184 8 based base VBN cord-001257-t21l6i3f 184 9 approaches approach NNS cord-001257-t21l6i3f 184 10 confirm confirm VBP cord-001257-t21l6i3f 184 11 that that IN cord-001257-t21l6i3f 184 12 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 184 13 and and CC cord-001257-t21l6i3f 184 14 AtRH5 AtRH5 NNP cord-001257-t21l6i3f 184 15 and and CC cord-001257-t21l6i3f 184 16 the the DT cord-001257-t21l6i3f 184 17 ortologous ortologous JJ cord-001257-t21l6i3f 184 18 yeast yeast NN cord-001257-t21l6i3f 184 19 helicases helicase NNS cord-001257-t21l6i3f 184 20 are be VBP cord-001257-t21l6i3f 184 21 important important JJ cord-001257-t21l6i3f 184 22 for for IN cord-001257-t21l6i3f 184 23 ( ( -LRB- cord-001257-t21l6i3f 184 24 + + CD cord-001257-t21l6i3f 184 25 ) ) -RRB- cord-001257-t21l6i3f 184 26 RNA rna NN cord-001257-t21l6i3f 184 27 synthesis synthesis NN cord-001257-t21l6i3f 184 28 during during IN cord-001257-t21l6i3f 184 29 TBSV TBSV NNP cord-001257-t21l6i3f 184 30 replication replication NN cord-001257-t21l6i3f 184 31 in in IN cord-001257-t21l6i3f 184 32 vitro vitro FW cord-001257-t21l6i3f 184 33 . . . cord-001257-t21l6i3f 185 1 To to TO cord-001257-t21l6i3f 185 2 examine examine VB cord-001257-t21l6i3f 185 3 if if IN cord-001257-t21l6i3f 185 4 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 185 5 and and CC cord-001257-t21l6i3f 185 6 AtRH5 atrh5 NN cord-001257-t21l6i3f 185 7 helicases helicase NNS cord-001257-t21l6i3f 185 8 are be VBP cord-001257-t21l6i3f 185 9 present present JJ cord-001257-t21l6i3f 185 10 within within IN cord-001257-t21l6i3f 185 11 the the DT cord-001257-t21l6i3f 185 12 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 185 13 replicase replicase NNP cord-001257-t21l6i3f 185 14 complex complex NNP cord-001257-t21l6i3f 185 15 , , , cord-001257-t21l6i3f 185 16 we -PRON- PRP cord-001257-t21l6i3f 185 17 FLAG flag VBP cord-001257-t21l6i3f 185 18 affinity affinity NN cord-001257-t21l6i3f 185 19 - - HYPH cord-001257-t21l6i3f 185 20 purified purify VBN cord-001257-t21l6i3f 185 21 the the DT cord-001257-t21l6i3f 185 22 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 185 23 replicase replicase NN cord-001257-t21l6i3f 185 24 from from IN cord-001257-t21l6i3f 185 25 yeast yeast NN cord-001257-t21l6i3f 185 26 cells cell NNS cord-001257-t21l6i3f 185 27 actively actively RB cord-001257-t21l6i3f 185 28 replicating replicate VBG cord-001257-t21l6i3f 185 29 TBSV TBSV NNP cord-001257-t21l6i3f 185 30 repRNA repRNA NNP cord-001257-t21l6i3f 185 31 [ [ -LRB- cord-001257-t21l6i3f 185 32 15 15 CD cord-001257-t21l6i3f 185 33 , , , cord-001257-t21l6i3f 185 34 28 28 CD cord-001257-t21l6i3f 185 35 ] ] -RRB- cord-001257-t21l6i3f 185 36 . . . cord-001257-t21l6i3f 186 1 The the DT cord-001257-t21l6i3f 186 2 yeast yeast NN cord-001257-t21l6i3f 186 3 cells cell NNS cord-001257-t21l6i3f 186 4 also also RB cord-001257-t21l6i3f 186 5 expressed express VBD cord-001257-t21l6i3f 186 6 either either CC cord-001257-t21l6i3f 186 7 His -PRON- PRP$ cord-001257-t21l6i3f 186 8 6 6 CD cord-001257-t21l6i3f 186 9 -tagged -tagged CD cord-001257-t21l6i3f 186 10 AtRH2 atrh2 NN cord-001257-t21l6i3f 187 1 or or CC cord-001257-t21l6i3f 187 2 His -PRON- PRP$ cord-001257-t21l6i3f 187 3 6 6 CD cord-001257-t21l6i3f 187 4 -AtRH5 -atrh5 NN cord-001257-t21l6i3f 187 5 helicases helicase NNS cord-001257-t21l6i3f 187 6 from from IN cord-001257-t21l6i3f 187 7 plasmids plasmid NNS cord-001257-t21l6i3f 187 8 . . . cord-001257-t21l6i3f 188 1 We -PRON- PRP cord-001257-t21l6i3f 188 2 found find VBD cord-001257-t21l6i3f 188 3 that that IN cord-001257-t21l6i3f 188 4 the the DT cord-001257-t21l6i3f 188 5 solubilized solubilized JJ cord-001257-t21l6i3f 188 6 and and CC cord-001257-t21l6i3f 188 7 affinity affinity NN cord-001257-t21l6i3f 188 8 - - HYPH cord-001257-t21l6i3f 188 9 purified purify VBN cord-001257-t21l6i3f 188 10 tombusvirus tombusvirus NN cord-001257-t21l6i3f 188 11 replicase replicase NN cord-001257-t21l6i3f 188 12 preparation preparation NN cord-001257-t21l6i3f 188 13 , , , cord-001257-t21l6i3f 188 14 which which WDT cord-001257-t21l6i3f 188 15 is be VBZ cord-001257-t21l6i3f 188 16 highly highly RB cord-001257-t21l6i3f 188 17 active active JJ cord-001257-t21l6i3f 188 18 on on IN cord-001257-t21l6i3f 188 19 added add VBN cord-001257-t21l6i3f 188 20 templates template NNS cord-001257-t21l6i3f 188 21 in in IN cord-001257-t21l6i3f 188 22 vitro vitro FW cord-001257-t21l6i3f 188 23 ( ( -LRB- cord-001257-t21l6i3f 188 24 not not RB cord-001257-t21l6i3f 188 25 shown show VBN cord-001257-t21l6i3f 188 26 ) ) -RRB- cord-001257-t21l6i3f 188 27 , , , cord-001257-t21l6i3f 188 28 contained contain VBD cord-001257-t21l6i3f 188 29 His -PRON- PRP$ cord-001257-t21l6i3f 188 30 6 6 CD cord-001257-t21l6i3f 188 31 -AtRH2 -atrh2 CD cord-001257-t21l6i3f 189 1 ( ( -LRB- cord-001257-t21l6i3f 189 2 Fig Fig NNP cord-001257-t21l6i3f 189 3 . . . cord-001257-t21l6i3f 189 4 7A 7a NN cord-001257-t21l6i3f 189 5 , , , cord-001257-t21l6i3f 189 6 lane lane NN cord-001257-t21l6i3f 189 7 2 2 CD cord-001257-t21l6i3f 189 8 ) ) -RRB- cord-001257-t21l6i3f 189 9 , , , cord-001257-t21l6i3f 189 10 while while IN cord-001257-t21l6i3f 189 11 His -PRON- PRP$ cord-001257-t21l6i3f 189 12 6 6 CD cord-001257-t21l6i3f 189 13 -AtRH2 -atrh2 WP cord-001257-t21l6i3f 189 14 was be VBD cord-001257-t21l6i3f 189 15 undetectable undetectable JJ cord-001257-t21l6i3f 189 16 in in IN cord-001257-t21l6i3f 189 17 the the DT cord-001257-t21l6i3f 189 18 control control NN cord-001257-t21l6i3f 189 19 yeast yeast NN cord-001257-t21l6i3f 189 20 sample sample NN cord-001257-t21l6i3f 189 21 obtained obtain VBN cord-001257-t21l6i3f 189 22 using use VBG cord-001257-t21l6i3f 189 23 the the DT cord-001257-t21l6i3f 189 24 same same JJ cord-001257-t21l6i3f 189 25 affinity affinity NN cord-001257-t21l6i3f 189 26 purification purification NN cord-001257-t21l6i3f 189 27 ( ( -LRB- cord-001257-t21l6i3f 189 28 Fig Fig NNP cord-001257-t21l6i3f 189 29 . . . cord-001257-t21l6i3f 189 30 7A 7a NN cord-001257-t21l6i3f 189 31 , , , cord-001257-t21l6i3f 189 32 lane lane NN cord-001257-t21l6i3f 189 33 3 3 CD cord-001257-t21l6i3f 189 34 ) ) -RRB- cord-001257-t21l6i3f 189 35 . . . cord-001257-t21l6i3f 190 1 Formation formation NN cord-001257-t21l6i3f 190 2 of of IN cord-001257-t21l6i3f 190 3 active active JJ cord-001257-t21l6i3f 190 4 replicase replicase NN cord-001257-t21l6i3f 190 5 complex complex NN cord-001257-t21l6i3f 190 6 was be VBD cord-001257-t21l6i3f 190 7 not not RB cord-001257-t21l6i3f 190 8 necessary necessary JJ cord-001257-t21l6i3f 190 9 for for IN cord-001257-t21l6i3f 190 10 His -PRON- PRP$ cord-001257-t21l6i3f 190 11 6 6 CD cord-001257-t21l6i3f 190 12 -AtRH2 -atrh2 CD cord-001257-t21l6i3f 190 13 to to TO cord-001257-t21l6i3f 190 14 become become VB cord-001257-t21l6i3f 190 15 co co JJ cord-001257-t21l6i3f 190 16 - - VBN cord-001257-t21l6i3f 190 17 opted opt VBN cord-001257-t21l6i3f 190 18 since since IN cord-001257-t21l6i3f 190 19 the the DT cord-001257-t21l6i3f 190 20 inactive inactive JJ cord-001257-t21l6i3f 190 21 purified purify VBN cord-001257-t21l6i3f 190 22 replicase replicase NN cord-001257-t21l6i3f 190 23 [ [ -LRB- cord-001257-t21l6i3f 190 24 the the DT cord-001257-t21l6i3f 190 25 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 190 26 replicase replicase NNP cord-001257-t21l6i3f 190 27 is be VBZ cord-001257-t21l6i3f 190 28 inactive inactive JJ cord-001257-t21l6i3f 190 29 in in IN cord-001257-t21l6i3f 190 30 the the DT cord-001257-t21l6i3f 190 31 absence absence NN cord-001257-t21l6i3f 190 32 of of IN cord-001257-t21l6i3f 190 33 the the DT cord-001257-t21l6i3f 190 34 viral viral JJ cord-001257-t21l6i3f 190 35 RNA RNA NNP cord-001257-t21l6i3f 190 36 ; ; : cord-001257-t21l6i3f 190 37 [ [ -LRB- cord-001257-t21l6i3f 190 38 19 19 CD cord-001257-t21l6i3f 190 39 , , , cord-001257-t21l6i3f 190 40 63 63 CD cord-001257-t21l6i3f 190 41 ] ] -RRB- cord-001257-t21l6i3f 190 42 ] ] -RRB- cord-001257-t21l6i3f 191 1 contained contain VBD cord-001257-t21l6i3f 191 2 His -PRON- PRP$ cord-001257-t21l6i3f 191 3 6 6 CD cord-001257-t21l6i3f 191 4 -AtRH2 -atrh2 CD cord-001257-t21l6i3f 192 1 when when WRB cord-001257-t21l6i3f 192 2 derived derive VBN cord-001257-t21l6i3f 192 3 from from IN cord-001257-t21l6i3f 192 4 yeast yeast NN cord-001257-t21l6i3f 192 5 lacking lack VBG cord-001257-t21l6i3f 192 6 the the DT cord-001257-t21l6i3f 192 7 viral viral JJ cord-001257-t21l6i3f 192 8 repRNA repRNA NNS cord-001257-t21l6i3f 193 1 ( ( -LRB- cord-001257-t21l6i3f 193 2 Fig Fig NNP cord-001257-t21l6i3f 193 3 . . . cord-001257-t21l6i3f 193 4 7A 7a NN cord-001257-t21l6i3f 193 5 , , , cord-001257-t21l6i3f 193 6 lane lane NN cord-001257-t21l6i3f 193 7 1 1 CD cord-001257-t21l6i3f 193 8 ) ) -RRB- cord-001257-t21l6i3f 193 9 . . . cord-001257-t21l6i3f 194 1 We -PRON- PRP cord-001257-t21l6i3f 194 2 found find VBD cord-001257-t21l6i3f 194 3 that that IN cord-001257-t21l6i3f 194 4 His -PRON- PRP$ cord-001257-t21l6i3f 194 5 6 6 CD cord-001257-t21l6i3f 194 6 -AtRH5 -atrh5 RB cord-001257-t21l6i3f 194 7 showed show VBD cord-001257-t21l6i3f 194 8 similar similar JJ cord-001257-t21l6i3f 194 9 characteristics characteristic NNS cord-001257-t21l6i3f 194 10 in in IN cord-001257-t21l6i3f 194 11 these these DT cord-001257-t21l6i3f 194 12 co co JJ cord-001257-t21l6i3f 194 13 - - JJ cord-001257-t21l6i3f 194 14 purification purification JJ cord-001257-t21l6i3f 194 15 experiments experiment NNS cord-001257-t21l6i3f 194 16 ( ( -LRB- cord-001257-t21l6i3f 194 17 Fig Fig NNP cord-001257-t21l6i3f 194 18 . . . cord-001257-t21l6i3f 194 19 S5A s5a CD cord-001257-t21l6i3f 194 20 ) ) -RRB- cord-001257-t21l6i3f 194 21 . . . cord-001257-t21l6i3f 195 1 To to TO cord-001257-t21l6i3f 195 2 test test VB cord-001257-t21l6i3f 195 3 if if IN cord-001257-t21l6i3f 195 4 the the DT cord-001257-t21l6i3f 195 5 TBSV TBSV NNP cord-001257-t21l6i3f 195 6 p33 p33 CD cord-001257-t21l6i3f 195 7 replication replication NN cord-001257-t21l6i3f 195 8 protein protein NN cord-001257-t21l6i3f 195 9 interacts interact VBZ cord-001257-t21l6i3f 195 10 directly directly RB cord-001257-t21l6i3f 195 11 with with IN cord-001257-t21l6i3f 195 12 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 195 13 and and CC cord-001257-t21l6i3f 195 14 AtRH5 AtRH5 NNP cord-001257-t21l6i3f 195 15 , , , cord-001257-t21l6i3f 195 16 we -PRON- PRP cord-001257-t21l6i3f 195 17 performed perform VBD cord-001257-t21l6i3f 195 18 membrane membrane NN cord-001257-t21l6i3f 195 19 - - HYPH cord-001257-t21l6i3f 195 20 based base VBN cord-001257-t21l6i3f 195 21 splitubiquitin splitubiquitin NNP cord-001257-t21l6i3f 195 22 yeast yeast NNP cord-001257-t21l6i3f 195 23 two two CD cord-001257-t21l6i3f 195 24 - - HYPH cord-001257-t21l6i3f 195 25 hybrid hybrid NN cord-001257-t21l6i3f 195 26 assay assay NN cord-001257-t21l6i3f 195 27 . . . cord-001257-t21l6i3f 196 1 This this DT cord-001257-t21l6i3f 196 2 assay assay NN cord-001257-t21l6i3f 196 3 confirmed confirm VBD cord-001257-t21l6i3f 196 4 the the DT cord-001257-t21l6i3f 196 5 interaction interaction NN cord-001257-t21l6i3f 196 6 between between IN cord-001257-t21l6i3f 196 7 p33 p33 CD cord-001257-t21l6i3f 196 8 and and CC cord-001257-t21l6i3f 196 9 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 196 10 and and CC cord-001257-t21l6i3f 196 11 AtRH5 atrh5 NN cord-001257-t21l6i3f 197 1 ( ( -LRB- cord-001257-t21l6i3f 197 2 Fig Fig NNP cord-001257-t21l6i3f 197 3 . . . cord-001257-t21l6i3f 197 4 7B 7B NNP cord-001257-t21l6i3f 197 5 ) ) -RRB- cord-001257-t21l6i3f 197 6 . . . cord-001257-t21l6i3f 198 1 The the DT cord-001257-t21l6i3f 198 2 yeast yeast NN cord-001257-t21l6i3f 198 3 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 198 4 and and CC cord-001257-t21l6i3f 198 5 Fal1p Fal1p NNP cord-001257-t21l6i3f 198 6 DEAD DEAD NNP cord-001257-t21l6i3f 198 7 - - HYPH cord-001257-t21l6i3f 198 8 box box NN cord-001257-t21l6i3f 198 9 helicases helicase NNS cord-001257-t21l6i3f 198 10 also also RB cord-001257-t21l6i3f 198 11 interacted interact VBD cord-001257-t21l6i3f 198 12 with with IN cord-001257-t21l6i3f 198 13 p33 p33 CD cord-001257-t21l6i3f 198 14 in in IN cord-001257-t21l6i3f 198 15 this this DT cord-001257-t21l6i3f 198 16 assay assay NN cord-001257-t21l6i3f 198 17 ( ( -LRB- cord-001257-t21l6i3f 198 18 Fig Fig NNP cord-001257-t21l6i3f 198 19 . . . cord-001257-t21l6i3f 198 20 S5B S5B NNP cord-001257-t21l6i3f 198 21 ) ) -RRB- cord-001257-t21l6i3f 198 22 . . . cord-001257-t21l6i3f 199 1 To to TO cord-001257-t21l6i3f 199 2 test test VB cord-001257-t21l6i3f 199 3 what what WP cord-001257-t21l6i3f 199 4 region region NN cord-001257-t21l6i3f 199 5 within within IN cord-001257-t21l6i3f 199 6 the the DT cord-001257-t21l6i3f 199 7 TBSV TBSV NNP cord-001257-t21l6i3f 199 8 p33 p33 CD cord-001257-t21l6i3f 199 9 replication replication NN cord-001257-t21l6i3f 199 10 protein protein NN cord-001257-t21l6i3f 199 11 is be VBZ cord-001257-t21l6i3f 199 12 involved involve VBN cord-001257-t21l6i3f 199 13 in in IN cord-001257-t21l6i3f 199 14 the the DT cord-001257-t21l6i3f 199 15 interaction interaction NN cord-001257-t21l6i3f 199 16 with with IN cord-001257-t21l6i3f 199 17 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 199 18 and and CC cord-001257-t21l6i3f 199 19 AtRH5 AtRH5 NNP cord-001257-t21l6i3f 199 20 , , , cord-001257-t21l6i3f 199 21 we -PRON- PRP cord-001257-t21l6i3f 199 22 performed perform VBD cord-001257-t21l6i3f 199 23 pull pull VB cord-001257-t21l6i3f 199 24 - - HYPH cord-001257-t21l6i3f 199 25 down down RP cord-001257-t21l6i3f 199 26 experiments experiment NNS cord-001257-t21l6i3f 199 27 with with IN cord-001257-t21l6i3f 199 28 MBP MBP NNP cord-001257-t21l6i3f 199 29 - - HYPH cord-001257-t21l6i3f 199 30 tagged tag VBN cord-001257-t21l6i3f 199 31 p33 p33 NNP cord-001257-t21l6i3f 199 32 derivatives derivative NNS cord-001257-t21l6i3f 199 33 from from IN cord-001257-t21l6i3f 199 34 E. E. NNP cord-001257-t21l6i3f 199 35 coli coli NNS cord-001257-t21l6i3f 199 36 . . . cord-001257-t21l6i3f 200 1 These these DT cord-001257-t21l6i3f 200 2 experiments experiment NNS cord-001257-t21l6i3f 200 3 revealed reveal VBD cord-001257-t21l6i3f 200 4 that that IN cord-001257-t21l6i3f 200 5 the the DT cord-001257-t21l6i3f 200 6 RPR rpr NN cord-001257-t21l6i3f 200 7 - - HYPH cord-001257-t21l6i3f 200 8 motif motif NN cord-001257-t21l6i3f 200 9 in in IN cord-001257-t21l6i3f 200 10 p33 p33 NN cord-001257-t21l6i3f 200 11 involved involve VBN cord-001257-t21l6i3f 200 12 in in IN cord-001257-t21l6i3f 200 13 viral viral JJ cord-001257-t21l6i3f 200 14 RNA RNA NNP cord-001257-t21l6i3f 200 15 - - HYPH cord-001257-t21l6i3f 200 16 binding binding NN cord-001257-t21l6i3f 200 17 was be VBD cord-001257-t21l6i3f 200 18 responsible responsible JJ cord-001257-t21l6i3f 200 19 for for IN cord-001257-t21l6i3f 200 20 interacting interact VBG cord-001257-t21l6i3f 200 21 with with IN cord-001257-t21l6i3f 200 22 both both CC cord-001257-t21l6i3f 200 23 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 200 24 and and CC cord-001257-t21l6i3f 200 25 AtRH5 atrh5 NN cord-001257-t21l6i3f 201 1 ( ( -LRB- cord-001257-t21l6i3f 201 2 Fig Fig NNP cord-001257-t21l6i3f 201 3 . . . cord-001257-t21l6i3f 201 4 S6A s6a NN cord-001257-t21l6i3f 201 5 - - HYPH cord-001257-t21l6i3f 201 6 B b NN cord-001257-t21l6i3f 201 7 ) ) -RRB- cord-001257-t21l6i3f 201 8 . . . cord-001257-t21l6i3f 202 1 Interestingly interestingly RB cord-001257-t21l6i3f 202 2 , , , cord-001257-t21l6i3f 202 3 the the DT cord-001257-t21l6i3f 202 4 interaction interaction NN cord-001257-t21l6i3f 202 5 of of IN cord-001257-t21l6i3f 202 6 p33 p33 CD cord-001257-t21l6i3f 202 7 with with IN cord-001257-t21l6i3f 202 8 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 202 9 and and CC cord-001257-t21l6i3f 202 10 AtRH5 AtRH5 NNP cord-001257-t21l6i3f 202 11 did do VBD cord-001257-t21l6i3f 202 12 not not RB cord-001257-t21l6i3f 202 13 affect affect VB cord-001257-t21l6i3f 202 14 the the DT cord-001257-t21l6i3f 202 15 ability ability NN cord-001257-t21l6i3f 202 16 of of IN cord-001257-t21l6i3f 202 17 p33 p33 NN cord-001257-t21l6i3f 202 18 to to TO cord-001257-t21l6i3f 202 19 bind bind VB cord-001257-t21l6i3f 202 20 to to IN cord-001257-t21l6i3f 202 21 the the DT cord-001257-t21l6i3f 202 22 viral viral NN cord-001257-t21l6i3f 202 23 ( ( -LRB- cord-001257-t21l6i3f 202 24 + + NNS cord-001257-t21l6i3f 202 25 ) ) -RRB- cord-001257-t21l6i3f 202 26 repRNA repRNA NNS cord-001257-t21l6i3f 203 1 in in IN cord-001257-t21l6i3f 203 2 vitro vitro FW cord-001257-t21l6i3f 203 3 ( ( -LRB- cord-001257-t21l6i3f 204 1 Fig Fig NNP cord-001257-t21l6i3f 204 2 . . . cord-001257-t21l6i3f 204 3 _SP cord-001257-t21l6i3f 204 4 S7 S7 NNP cord-001257-t21l6i3f 204 5 ) ) -RRB- cord-001257-t21l6i3f 204 6 . . . cord-001257-t21l6i3f 205 1 The the DT cord-001257-t21l6i3f 205 2 interaction interaction NN cord-001257-t21l6i3f 205 3 between between IN cord-001257-t21l6i3f 205 4 p33 p33 CD cord-001257-t21l6i3f 205 5 and and CC cord-001257-t21l6i3f 205 6 ( ( -LRB- cord-001257-t21l6i3f 205 7 + + NNS cord-001257-t21l6i3f 205 8 ) ) -RRB- cord-001257-t21l6i3f 205 9 repRNA repRNA NNP cord-001257-t21l6i3f 205 10 is be VBZ cord-001257-t21l6i3f 205 11 required require VBN cord-001257-t21l6i3f 205 12 for for IN cord-001257-t21l6i3f 205 13 recruitment recruitment NN cord-001257-t21l6i3f 205 14 of of IN cord-001257-t21l6i3f 205 15 the the DT cord-001257-t21l6i3f 205 16 viral viral JJ cord-001257-t21l6i3f 205 17 ( ( -LRB- cord-001257-t21l6i3f 205 18 + + CC cord-001257-t21l6i3f 205 19 ) ) -RRB- cord-001257-t21l6i3f 205 20 RNA RNA NNP cord-001257-t21l6i3f 205 21 into into IN cord-001257-t21l6i3f 205 22 replication replication NN cord-001257-t21l6i3f 205 23 [ [ -LRB- cord-001257-t21l6i3f 205 24 17 17 CD cord-001257-t21l6i3f 205 25 , , , cord-001257-t21l6i3f 205 26 64 64 CD cord-001257-t21l6i3f 205 27 ] ] -RRB- cord-001257-t21l6i3f 205 28 . . . cord-001257-t21l6i3f 206 1 Based base VBN cord-001257-t21l6i3f 206 2 on on IN cord-001257-t21l6i3f 206 3 the the DT cord-001257-t21l6i3f 206 4 above above JJ cord-001257-t21l6i3f 206 5 interaction interaction NN cord-001257-t21l6i3f 206 6 data datum NNS cord-001257-t21l6i3f 206 7 , , , cord-001257-t21l6i3f 206 8 we -PRON- PRP cord-001257-t21l6i3f 206 9 suggest suggest VBP cord-001257-t21l6i3f 206 10 that that IN cord-001257-t21l6i3f 206 11 the the DT cord-001257-t21l6i3f 206 12 viral viral JJ cord-001257-t21l6i3f 206 13 p33 p33 CD cord-001257-t21l6i3f 206 14 replication replication NN cord-001257-t21l6i3f 206 15 protein protein NN cord-001257-t21l6i3f 206 16 ( ( -LRB- cord-001257-t21l6i3f 206 17 and and CC cord-001257-t21l6i3f 206 18 p92 p92 NN cord-001257-t21l6i3f 206 19 replication replication NN cord-001257-t21l6i3f 206 20 protein protein NN cord-001257-t21l6i3f 206 21 , , , cord-001257-t21l6i3f 206 22 which which WDT cord-001257-t21l6i3f 206 23 within within IN cord-001257-t21l6i3f 206 24 its -PRON- PRP$ cord-001257-t21l6i3f 206 25 N n CD cord-001257-t21l6i3f 206 26 - - HYPH cord-001257-t21l6i3f 206 27 terminal terminal NN cord-001257-t21l6i3f 206 28 region region NN cord-001257-t21l6i3f 206 29 contains contain VBZ cord-001257-t21l6i3f 206 30 the the DT cord-001257-t21l6i3f 206 31 p33 p33 CD cord-001257-t21l6i3f 206 32 sequence sequence NN cord-001257-t21l6i3f 206 33 due due IN cord-001257-t21l6i3f 206 34 to to IN cord-001257-t21l6i3f 206 35 the the DT cord-001257-t21l6i3f 206 36 expression expression NN cord-001257-t21l6i3f 206 37 strategy strategy NN cord-001257-t21l6i3f 206 38 ) ) -RRB- cord-001257-t21l6i3f 206 39 co co NNS cord-001257-t21l6i3f 206 40 - - NN cord-001257-t21l6i3f 206 41 opts opt VBZ cord-001257-t21l6i3f 206 42 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 206 43 and and CC cord-001257-t21l6i3f 206 44 AtRH5 atrh5 RB cord-001257-t21l6i3f 206 45 DEAD DEAD NNP cord-001257-t21l6i3f 206 46 - - HYPH cord-001257-t21l6i3f 206 47 box box NN cord-001257-t21l6i3f 206 48 proteins protein NNS cord-001257-t21l6i3f 206 49 from from IN cord-001257-t21l6i3f 206 50 the the DT cord-001257-t21l6i3f 206 51 host host NN cord-001257-t21l6i3f 206 52 cells cell NNS cord-001257-t21l6i3f 206 53 into into IN cord-001257-t21l6i3f 206 54 the the DT cord-001257-t21l6i3f 206 55 viral viral JJ cord-001257-t21l6i3f 206 56 replicase replicase NN cord-001257-t21l6i3f 206 57 complexes complex NNS cord-001257-t21l6i3f 206 58 to to TO cord-001257-t21l6i3f 206 59 aid aid VB cord-001257-t21l6i3f 206 60 the the DT cord-001257-t21l6i3f 206 61 replication replication NN cord-001257-t21l6i3f 206 62 process process NN cord-001257-t21l6i3f 206 63 . . . cord-001257-t21l6i3f 207 1 The the DT cord-001257-t21l6i3f 207 2 difference difference NN cord-001257-t21l6i3f 207 3 in in IN cord-001257-t21l6i3f 207 4 viral viral JJ cord-001257-t21l6i3f 207 5 RNA rna NN cord-001257-t21l6i3f 207 6 - - HYPH cord-001257-t21l6i3f 207 7 binding binding NN cord-001257-t21l6i3f 207 8 by by IN cord-001257-t21l6i3f 207 9 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 207 10 / / , cord-001257-t21l6i3f 207 11 AtRH5 atrh5 NN cord-001257-t21l6i3f 207 12 versus versus IN cord-001257-t21l6i3f 207 13 AtRH20 atrh20 NN cord-001257-t21l6i3f 207 14 suggests suggest VBZ cord-001257-t21l6i3f 207 15 that that IN cord-001257-t21l6i3f 207 16 these these DT cord-001257-t21l6i3f 207 17 groups group NNS cord-001257-t21l6i3f 207 18 of of IN cord-001257-t21l6i3f 207 19 helicases helicase NNS cord-001257-t21l6i3f 207 20 could could MD cord-001257-t21l6i3f 207 21 have have VB cord-001257-t21l6i3f 207 22 synergistic synergistic JJ cord-001257-t21l6i3f 207 23 effect effect NN cord-001257-t21l6i3f 207 24 on on IN cord-001257-t21l6i3f 207 25 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 207 26 replication replication NN cord-001257-t21l6i3f 207 27 . . . cord-001257-t21l6i3f 208 1 This this DT cord-001257-t21l6i3f 208 2 was be VBD cord-001257-t21l6i3f 208 3 tested test VBN cord-001257-t21l6i3f 208 4 by by IN cord-001257-t21l6i3f 208 5 coexpressing coexpresse VBG cord-001257-t21l6i3f 208 6 AtRH5 atrh5 NN cord-001257-t21l6i3f 208 7 and and CC cord-001257-t21l6i3f 208 8 AtRH20 atrh20 NN cord-001257-t21l6i3f 209 1 in in IN cord-001257-t21l6i3f 209 2 N. N. NNP cord-001257-t21l6i3f 209 3 benthamiana benthamiana NNP cord-001257-t21l6i3f 209 4 leaves leave NNS cord-001257-t21l6i3f 209 5 replicat- replicat- RB cord-001257-t21l6i3f 210 1 ing e VBG cord-001257-t21l6i3f 210 2 the the DT cord-001257-t21l6i3f 210 3 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 210 4 RNA RNA NNP cord-001257-t21l6i3f 210 5 ( ( -LRB- cord-001257-t21l6i3f 211 1 Fig Fig NNP cord-001257-t21l6i3f 211 2 . . . cord-001257-t21l6i3f 211 3 8) 8) NNP cord-001257-t21l6i3f 211 4 . . . cord-001257-t21l6i3f 212 1 Interestingly interestingly RB cord-001257-t21l6i3f 212 2 , , , cord-001257-t21l6i3f 212 3 AtRH5 atrh5 NN cord-001257-t21l6i3f 212 4 and and CC cord-001257-t21l6i3f 212 5 AtRH20 AtRH20 NNP cord-001257-t21l6i3f 212 6 host host NN cord-001257-t21l6i3f 212 7 proteins protein NNS cord-001257-t21l6i3f 212 8 , , , cord-001257-t21l6i3f 212 9 when when WRB cord-001257-t21l6i3f 212 10 co co NN cord-001257-t21l6i3f 212 11 - - VBD cord-001257-t21l6i3f 212 12 expressed express VBN cord-001257-t21l6i3f 212 13 together together RB cord-001257-t21l6i3f 212 14 , , , cord-001257-t21l6i3f 212 15 had have VBD cord-001257-t21l6i3f 212 16 the the DT cord-001257-t21l6i3f 212 17 largest large JJS cord-001257-t21l6i3f 212 18 ( ( -LRB- cord-001257-t21l6i3f 212 19 up up IN cord-001257-t21l6i3f 212 20 to to IN cord-001257-t21l6i3f 212 21 , , , cord-001257-t21l6i3f 212 22 5.5-fold 5.5-fold CD cord-001257-t21l6i3f 212 23 , , , cord-001257-t21l6i3f 212 24 Fig Fig NNP cord-001257-t21l6i3f 212 25 . . . cord-001257-t21l6i3f 213 1 8A 8A NNP cord-001257-t21l6i3f 213 2 , , , cord-001257-t21l6i3f 213 3 lanes lane VBZ cord-001257-t21l6i3f 213 4 13 13 CD cord-001257-t21l6i3f 213 5 - - SYM cord-001257-t21l6i3f 213 6 16 16 CD cord-001257-t21l6i3f 213 7 ) ) -RRB- cord-001257-t21l6i3f 213 8 effect effect NN cord-001257-t21l6i3f 213 9 on on IN cord-001257-t21l6i3f 213 10 viral viral JJ cord-001257-t21l6i3f 213 11 genomic genomic JJ cord-001257-t21l6i3f 213 12 RNA RNA NNP cord-001257-t21l6i3f 213 13 accumulation accumulation NN cord-001257-t21l6i3f 213 14 in in IN cord-001257-t21l6i3f 213 15 comparison comparison NN cord-001257-t21l6i3f 213 16 with with IN cord-001257-t21l6i3f 213 17 the the DT cord-001257-t21l6i3f 213 18 , , , cord-001257-t21l6i3f 213 19 2-fold 2-fold CD cord-001257-t21l6i3f 213 20 increase increase NN cord-001257-t21l6i3f 213 21 for for IN cord-001257-t21l6i3f 213 22 separate separate JJ cord-001257-t21l6i3f 213 23 expression expression NN cord-001257-t21l6i3f 213 24 of of IN cord-001257-t21l6i3f 213 25 AtRH20 AtRH20 NNP cord-001257-t21l6i3f 213 26 ( ( -LRB- cord-001257-t21l6i3f 213 27 lanes lane NNS cord-001257-t21l6i3f 213 28 5 5 CD cord-001257-t21l6i3f 213 29 - - SYM cord-001257-t21l6i3f 213 30 8 8 CD cord-001257-t21l6i3f 213 31 ) ) -RRB- cord-001257-t21l6i3f 213 32 and and CC cord-001257-t21l6i3f 213 33 AtRH5 AtRH5 NNP cord-001257-t21l6i3f 213 34 ( ( -LRB- cord-001257-t21l6i3f 213 35 lanes lane NNS cord-001257-t21l6i3f 213 36 9 9 CD cord-001257-t21l6i3f 213 37 - - SYM cord-001257-t21l6i3f 213 38 12 12 CD cord-001257-t21l6i3f 213 39 ) ) -RRB- cord-001257-t21l6i3f 213 40 . . . cord-001257-t21l6i3f 214 1 Also also RB cord-001257-t21l6i3f 214 2 , , , cord-001257-t21l6i3f 214 3 the the DT cord-001257-t21l6i3f 214 4 symptom symptom NN cord-001257-t21l6i3f 214 5 development development NN cord-001257-t21l6i3f 214 6 of of IN cord-001257-t21l6i3f 214 7 tombusvirus tombusvirus NN cord-001257-t21l6i3f 214 8 - - HYPH cord-001257-t21l6i3f 214 9 infected infect VBN cord-001257-t21l6i3f 214 10 plants plant NNS cord-001257-t21l6i3f 214 11 was be VBD cord-001257-t21l6i3f 214 12 the the DT cord-001257-t21l6i3f 214 13 most most RBS cord-001257-t21l6i3f 214 14 severe severe JJ cord-001257-t21l6i3f 214 15 and and CC cord-001257-t21l6i3f 214 16 the the DT cord-001257-t21l6i3f 214 17 fastest fast JJS cord-001257-t21l6i3f 214 18 when when WRB cord-001257-t21l6i3f 214 19 the the DT cord-001257-t21l6i3f 214 20 two two CD cord-001257-t21l6i3f 214 21 helicases helicase NNS cord-001257-t21l6i3f 214 22 were be VBD cord-001257-t21l6i3f 214 23 co co VBN cord-001257-t21l6i3f 214 24 - - VBN cord-001257-t21l6i3f 214 25 expressed express VBN cord-001257-t21l6i3f 214 26 ( ( -LRB- cord-001257-t21l6i3f 214 27 Fig Fig NNP cord-001257-t21l6i3f 214 28 . . . cord-001257-t21l6i3f 214 29 8C 8c CD cord-001257-t21l6i3f 214 30 ) ) -RRB- cord-001257-t21l6i3f 214 31 . . . cord-001257-t21l6i3f 215 1 Based base VBN cord-001257-t21l6i3f 215 2 on on IN cord-001257-t21l6i3f 215 3 these these DT cord-001257-t21l6i3f 215 4 data datum NNS cord-001257-t21l6i3f 215 5 , , , cord-001257-t21l6i3f 215 6 we -PRON- PRP cord-001257-t21l6i3f 215 7 suggest suggest VBP cord-001257-t21l6i3f 215 8 that that IN cord-001257-t21l6i3f 215 9 AtRH5 atrh5 NN cord-001257-t21l6i3f 215 10 and and CC cord-001257-t21l6i3f 215 11 AtRH20 AtRH20 NNP cord-001257-t21l6i3f 215 12 have have VB cord-001257-t21l6i3f 215 13 a a DT cord-001257-t21l6i3f 215 14 synergistic synergistic JJ cord-001257-t21l6i3f 215 15 effect effect NN cord-001257-t21l6i3f 215 16 on on IN cord-001257-t21l6i3f 215 17 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 215 18 replication replication NN cord-001257-t21l6i3f 215 19 ( ( -LRB- cord-001257-t21l6i3f 215 20 see see VB cord-001257-t21l6i3f 215 21 further further JJ cord-001257-t21l6i3f 215 22 explanation explanation NN cord-001257-t21l6i3f 215 23 in in IN cord-001257-t21l6i3f 215 24 discussion discussion NN cord-001257-t21l6i3f 215 25 ) ) -RRB- cord-001257-t21l6i3f 215 26 . . . cord-001257-t21l6i3f 216 1 Replication replication NN cord-001257-t21l6i3f 216 2 of of IN cord-001257-t21l6i3f 216 3 ( ( -LRB- cord-001257-t21l6i3f 216 4 + + CC cord-001257-t21l6i3f 216 5 ) ) -RRB- cord-001257-t21l6i3f 216 6 RNA RNA NNP cord-001257-t21l6i3f 216 7 viruses virus NNS cord-001257-t21l6i3f 216 8 is be VBZ cord-001257-t21l6i3f 216 9 performed perform VBN cord-001257-t21l6i3f 216 10 by by IN cord-001257-t21l6i3f 216 11 viral viral JJ cord-001257-t21l6i3f 216 12 replicases replicase NNS cord-001257-t21l6i3f 216 13 ( ( -LRB- cord-001257-t21l6i3f 216 14 VRCs VRCs NNP cord-001257-t21l6i3f 216 15 ) ) -RRB- cord-001257-t21l6i3f 216 16 , , , cord-001257-t21l6i3f 216 17 which which WDT cord-001257-t21l6i3f 216 18 are be VBP cord-001257-t21l6i3f 216 19 membrane membrane NN cord-001257-t21l6i3f 216 20 - - HYPH cord-001257-t21l6i3f 216 21 bound bind VBN cord-001257-t21l6i3f 216 22 ribonucleic ribonucleic JJ cord-001257-t21l6i3f 216 23 acid acid NN cord-001257-t21l6i3f 216 24 - - HYPH cord-001257-t21l6i3f 216 25 protein protein NN cord-001257-t21l6i3f 216 26 complexes complex NNS cord-001257-t21l6i3f 216 27 ( ( -LRB- cord-001257-t21l6i3f 216 28 RNP RNP NNP cord-001257-t21l6i3f 216 29 ) ) -RRB- cord-001257-t21l6i3f 216 30 [ [ -LRB- cord-001257-t21l6i3f 216 31 4 4 CD cord-001257-t21l6i3f 216 32 , , , cord-001257-t21l6i3f 216 33 12 12 CD cord-001257-t21l6i3f 216 34 , , , cord-001257-t21l6i3f 216 35 65 65 CD cord-001257-t21l6i3f 216 36 ] ] -RRB- cord-001257-t21l6i3f 216 37 . . . cord-001257-t21l6i3f 217 1 The the DT cord-001257-t21l6i3f 217 2 VRCs vrc NNS cord-001257-t21l6i3f 217 3 likely likely RB cord-001257-t21l6i3f 217 4 have have VBP cord-001257-t21l6i3f 217 5 to to TO cord-001257-t21l6i3f 217 6 remodel remodel VB cord-001257-t21l6i3f 217 7 viral viral JJ cord-001257-t21l6i3f 217 8 RNA RNA NNP cord-001257-t21l6i3f 217 9 structures structure NNS cord-001257-t21l6i3f 217 10 , , , cord-001257-t21l6i3f 217 11 including include VBG cord-001257-t21l6i3f 217 12 the the DT cord-001257-t21l6i3f 217 13 dsRNA dsrna NN cord-001257-t21l6i3f 217 14 formed form VBN cord-001257-t21l6i3f 217 15 during during IN cord-001257-t21l6i3f 217 16 replication replication NN cord-001257-t21l6i3f 217 17 . . . cord-001257-t21l6i3f 218 1 In in IN cord-001257-t21l6i3f 218 2 addition addition NN cord-001257-t21l6i3f 218 3 , , , cord-001257-t21l6i3f 218 4 the the DT cord-001257-t21l6i3f 218 5 viral viral JJ cord-001257-t21l6i3f 218 6 RNA RNA NNP cord-001257-t21l6i3f 218 7 plays play VBZ cord-001257-t21l6i3f 218 8 multiple multiple JJ cord-001257-t21l6i3f 218 9 roles role NNS cord-001257-t21l6i3f 218 10 , , , cord-001257-t21l6i3f 218 11 such such JJ cord-001257-t21l6i3f 218 12 as as IN cord-001257-t21l6i3f 218 13 template template NN cord-001257-t21l6i3f 218 14 for for IN cord-001257-t21l6i3f 218 15 translation translation NN cord-001257-t21l6i3f 218 16 and and CC cord-001257-t21l6i3f 218 17 RNA RNA NNP cord-001257-t21l6i3f 218 18 synthesis synthesis NN cord-001257-t21l6i3f 218 19 , , , cord-001257-t21l6i3f 218 20 and and CC cord-001257-t21l6i3f 218 21 as as IN cord-001257-t21l6i3f 218 22 a a DT cord-001257-t21l6i3f 218 23 VRC VRC NNP cord-001257-t21l6i3f 218 24 assembly assembly NN cord-001257-t21l6i3f 218 25 platform platform NN cord-001257-t21l6i3f 218 26 and and CC cord-001257-t21l6i3f 218 27 the the DT cord-001257-t21l6i3f 218 28 viral viral JJ cord-001257-t21l6i3f 218 29 RNA RNA NNP cord-001257-t21l6i3f 218 30 also also RB cord-001257-t21l6i3f 218 31 becomes become VBZ cord-001257-t21l6i3f 218 32 encapsidated encapsidate VBN cord-001257-t21l6i3f 218 33 during during IN cord-001257-t21l6i3f 218 34 infection infection NN cord-001257-t21l6i3f 218 35 [ [ -LRB- cord-001257-t21l6i3f 218 36 2 2 CD cord-001257-t21l6i3f 218 37 ] ] -RRB- cord-001257-t21l6i3f 218 38 [ [ -LRB- cord-001257-t21l6i3f 218 39 3 3 CD cord-001257-t21l6i3f 218 40 ] ] -RRB- cord-001257-t21l6i3f 218 41 [ [ -LRB- cord-001257-t21l6i3f 218 42 4 4 CD cord-001257-t21l6i3f 218 43 ] ] -RRB- cord-001257-t21l6i3f 218 44 66 66 CD cord-001257-t21l6i3f 218 45 ] ] -RRB- cord-001257-t21l6i3f 218 46 . . . cord-001257-t21l6i3f 219 1 It -PRON- PRP cord-001257-t21l6i3f 219 2 is be VBZ cord-001257-t21l6i3f 219 3 likely likely JJ cord-001257-t21l6i3f 219 4 that that IN cord-001257-t21l6i3f 219 5 remodeling remodeling NN cord-001257-t21l6i3f 219 6 of of IN cord-001257-t21l6i3f 219 7 the the DT cord-001257-t21l6i3f 219 8 viral viral JJ cord-001257-t21l6i3f 219 9 RNAs rna NNS cord-001257-t21l6i3f 219 10 and and CC cord-001257-t21l6i3f 219 11 RNP RNP NNP cord-001257-t21l6i3f 219 12 complexes complex NNS cord-001257-t21l6i3f 219 13 during during IN cord-001257-t21l6i3f 219 14 the the DT cord-001257-t21l6i3f 219 15 switch switch NN cord-001257-t21l6i3f 219 16 from from IN cord-001257-t21l6i3f 219 17 one one CD cord-001257-t21l6i3f 219 18 step step NN cord-001257-t21l6i3f 219 19 to to IN cord-001257-t21l6i3f 219 20 another another DT cord-001257-t21l6i3f 219 21 requires require VBZ cord-001257-t21l6i3f 219 22 RNA RNA NNP cord-001257-t21l6i3f 219 23 helicases helicase NNS cord-001257-t21l6i3f 219 24 or or CC cord-001257-t21l6i3f 219 25 RNA RNA NNP cord-001257-t21l6i3f 219 26 chaperones chaperone NNS cord-001257-t21l6i3f 219 27 . . . cord-001257-t21l6i3f 220 1 While while IN cord-001257-t21l6i3f 220 2 the the DT cord-001257-t21l6i3f 220 3 larger large JJR cord-001257-t21l6i3f 220 4 RNA RNA NNP cord-001257-t21l6i3f 220 5 viruses virus NNS cord-001257-t21l6i3f 220 6 over over IN cord-001257-t21l6i3f 220 7 6,000 6,000 CD cord-001257-t21l6i3f 220 8 nt nt RB cord-001257-t21l6i3f 220 9 genome genome NN cord-001257-t21l6i3f 220 10 - - HYPH cord-001257-t21l6i3f 220 11 size size NN cord-001257-t21l6i3f 220 12 all all DT cord-001257-t21l6i3f 220 13 code code NN cord-001257-t21l6i3f 220 14 for for IN cord-001257-t21l6i3f 220 15 RNA RNA NNP cord-001257-t21l6i3f 220 16 helicase helicase NNP cord-001257-t21l6i3f 220 17 - - HYPH cord-001257-t21l6i3f 220 18 like like JJ cord-001257-t21l6i3f 220 19 proteins protein NNS cord-001257-t21l6i3f 220 20 [ [ -LRB- cord-001257-t21l6i3f 220 21 47 47 CD cord-001257-t21l6i3f 220 22 , , , cord-001257-t21l6i3f 220 23 48 48 CD cord-001257-t21l6i3f 220 24 ] ] -RRB- cord-001257-t21l6i3f 220 25 , , , cord-001257-t21l6i3f 220 26 small small JJ cord-001257-t21l6i3f 220 27 RNA RNA NNP cord-001257-t21l6i3f 220 28 viruses virus NNS cord-001257-t21l6i3f 220 29 usually usually RB cord-001257-t21l6i3f 220 30 do do VBP cord-001257-t21l6i3f 220 31 not not RB cord-001257-t21l6i3f 220 32 code code VB cord-001257-t21l6i3f 220 33 for for IN cord-001257-t21l6i3f 220 34 RNA RNA NNP cord-001257-t21l6i3f 220 35 helicases helicase NNS cord-001257-t21l6i3f 220 36 . . . cord-001257-t21l6i3f 221 1 However however RB cord-001257-t21l6i3f 221 2 , , , cord-001257-t21l6i3f 221 3 the the DT cord-001257-t21l6i3f 221 4 small small JJ cord-001257-t21l6i3f 221 5 RNA RNA NNP cord-001257-t21l6i3f 221 6 viruses virus NNS cord-001257-t21l6i3f 221 7 likely likely RB cord-001257-t21l6i3f 221 8 coopt coopt VBP cord-001257-t21l6i3f 221 9 cellular cellular JJ cord-001257-t21l6i3f 221 10 RNA RNA NNP cord-001257-t21l6i3f 221 11 helicases helicase NNS cord-001257-t21l6i3f 221 12 during during IN cord-001257-t21l6i3f 221 13 infections infection NNS cord-001257-t21l6i3f 221 14 as as IN cord-001257-t21l6i3f 221 15 shown show VBN cord-001257-t21l6i3f 221 16 for for IN cord-001257-t21l6i3f 221 17 TBSV TBSV NNP cord-001257-t21l6i3f 221 18 [ [ -LRB- cord-001257-t21l6i3f 221 19 30 30 CD cord-001257-t21l6i3f 221 20 , , , cord-001257-t21l6i3f 221 21 49 49 CD cord-001257-t21l6i3f 221 22 ] ] -RRB- cord-001257-t21l6i3f 221 23 . . . cord-001257-t21l6i3f 222 1 The the DT cord-001257-t21l6i3f 222 2 yeast yeast NN cord-001257-t21l6i3f 222 3 DDX3-like DDX3-like NNP cord-001257-t21l6i3f 222 4 Ded1p Ded1p NNP cord-001257-t21l6i3f 222 5 and and CC cord-001257-t21l6i3f 222 6 the the DT cord-001257-t21l6i3f 222 7 orthologous orthologous JJ cord-001257-t21l6i3f 222 8 plant plant NN cord-001257-t21l6i3f 223 1 AtRH20 AtRH20 NNP cord-001257-t21l6i3f 223 2 helicases helicase NNS cord-001257-t21l6i3f 223 3 are be VBP cord-001257-t21l6i3f 223 4 recruited recruit VBN cord-001257-t21l6i3f 223 5 for for IN cord-001257-t21l6i3f 223 6 TBSV TBSV NNP cord-001257-t21l6i3f 223 7 replication replication NN cord-001257-t21l6i3f 223 8 to to TO cord-001257-t21l6i3f 223 9 promote promote VB cord-001257-t21l6i3f 223 10 ( ( -LRB- cord-001257-t21l6i3f 223 11 + + NN cord-001257-t21l6i3f 223 12 ) ) -RRB- cord-001257-t21l6i3f 224 1 -strand -strand DT cord-001257-t21l6i3f 224 2 RNA RNA NNP cord-001257-t21l6i3f 224 3 synthesis synthesis NN cord-001257-t21l6i3f 224 4 by by IN cord-001257-t21l6i3f 224 5 aiding aid VBG cord-001257-t21l6i3f 224 6 initiation initiation NN cord-001257-t21l6i3f 224 7 by by IN cord-001257-t21l6i3f 224 8 the the DT cord-001257-t21l6i3f 224 9 viral viral JJ cord-001257-t21l6i3f 224 10 RdRp RdRp NNP cord-001257-t21l6i3f 224 11 . . . cord-001257-t21l6i3f 225 1 However however RB cord-001257-t21l6i3f 225 2 , , , cord-001257-t21l6i3f 225 3 genome genome NN cord-001257-t21l6i3f 225 4 - - HYPH cord-001257-t21l6i3f 225 5 wide wide JJ cord-001257-t21l6i3f 225 6 screens screen NNS cord-001257-t21l6i3f 225 7 and and CC cord-001257-t21l6i3f 225 8 global global JJ cord-001257-t21l6i3f 225 9 proteomics proteomic NNS cord-001257-t21l6i3f 225 10 approaches approach NNS cord-001257-t21l6i3f 225 11 with with IN cord-001257-t21l6i3f 225 12 TBSV TBSV NNP cord-001257-t21l6i3f 225 13 have have VBP cord-001257-t21l6i3f 225 14 identified identify VBN cord-001257-t21l6i3f 225 15 11 11 CD cord-001257-t21l6i3f 225 16 host host NN cord-001257-t21l6i3f 225 17 helicases helicase NNS cord-001257-t21l6i3f 225 18 , , , cord-001257-t21l6i3f 225 19 suggesting suggest VBG cord-001257-t21l6i3f 225 20 that that IN cord-001257-t21l6i3f 225 21 several several JJ cord-001257-t21l6i3f 225 22 host host NN cord-001257-t21l6i3f 225 23 helicases helicase NNS cord-001257-t21l6i3f 225 24 might may MD cord-001257-t21l6i3f 225 25 be be VB cord-001257-t21l6i3f 225 26 co co VBN cord-001257-t21l6i3f 225 27 - - VBN cord-001257-t21l6i3f 225 28 opted opt VBN cord-001257-t21l6i3f 225 29 during during IN cord-001257-t21l6i3f 225 30 TBSV TBSV NNP cord-001257-t21l6i3f 225 31 infections infection NNS cord-001257-t21l6i3f 225 32 [ [ -LRB- cord-001257-t21l6i3f 225 33 28 28 CD cord-001257-t21l6i3f 225 34 , , , cord-001257-t21l6i3f 225 35 34 34 CD cord-001257-t21l6i3f 225 36 ] ] -RRB- cord-001257-t21l6i3f 225 37 . . . cord-001257-t21l6i3f 226 1 In in IN cord-001257-t21l6i3f 226 2 the the DT cord-001257-t21l6i3f 226 3 current current JJ cord-001257-t21l6i3f 226 4 work work NN cord-001257-t21l6i3f 226 5 , , , cord-001257-t21l6i3f 226 6 we -PRON- PRP cord-001257-t21l6i3f 226 7 have have VBP cord-001257-t21l6i3f 226 8 discovered discover VBN cord-001257-t21l6i3f 226 9 another another DT cord-001257-t21l6i3f 226 10 class class NN cord-001257-t21l6i3f 226 11 of of IN cord-001257-t21l6i3f 226 12 cellular cellular JJ cord-001257-t21l6i3f 226 13 DEAD DEAD NNP cord-001257-t21l6i3f 226 14 box box NN cord-001257-t21l6i3f 226 15 RNA RNA NNP cord-001257-t21l6i3f 226 16 helicases helicase NNS cord-001257-t21l6i3f 226 17 , , , cord-001257-t21l6i3f 226 18 including include VBG cord-001257-t21l6i3f 226 19 the the DT cord-001257-t21l6i3f 226 20 yeast yeast NN cord-001257-t21l6i3f 226 21 eIF4AIII eIF4AIII '' cord-001257-t21l6i3f 226 22 - - : cord-001257-t21l6i3f 226 23 like like IN cord-001257-t21l6i3f 226 24 Fal1p Fal1p NNP cord-001257-t21l6i3f 226 25 and and CC cord-001257-t21l6i3f 226 26 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 226 27 and and CC cord-001257-t21l6i3f 226 28 the the DT cord-001257-t21l6i3f 226 29 orthologous orthologous JJ cord-001257-t21l6i3f 226 30 plant plant NN cord-001257-t21l6i3f 226 31 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 226 32 and and CC cord-001257-t21l6i3f 226 33 DDX5-like DDX5-like NNP cord-001257-t21l6i3f 227 1 AtRH5 atrh5 RB cord-001257-t21l6i3f 227 2 DEAD dead JJ cord-001257-t21l6i3f 227 3 box box NN cord-001257-t21l6i3f 227 4 helicases helicase NNS cord-001257-t21l6i3f 227 5 , , , cord-001257-t21l6i3f 227 6 which which WDT cord-001257-t21l6i3f 227 7 are be VBP cord-001257-t21l6i3f 227 8 co co VBN cord-001257-t21l6i3f 227 9 - - VBN cord-001257-t21l6i3f 227 10 opted opt VBN cord-001257-t21l6i3f 227 11 by by IN cord-001257-t21l6i3f 227 12 tombusviruses tombusviruse NNS cord-001257-t21l6i3f 227 13 for for IN cord-001257-t21l6i3f 227 14 distinct distinct JJ cord-001257-t21l6i3f 227 15 pro pro JJ cord-001257-t21l6i3f 227 16 - - JJ cord-001257-t21l6i3f 227 17 viral viral JJ cord-001257-t21l6i3f 227 18 functions function NNS cord-001257-t21l6i3f 227 19 . . . cord-001257-t21l6i3f 228 1 eIF4AIII eIF4AIII NNS cord-001257-t21l6i3f 228 2 - - HYPH cord-001257-t21l6i3f 228 3 like like JJ cord-001257-t21l6i3f 228 4 helicases helicase NNS cord-001257-t21l6i3f 228 5 , , , cord-001257-t21l6i3f 228 6 which which WDT cord-001257-t21l6i3f 228 7 assist assist VBP cord-001257-t21l6i3f 228 8 ribosome ribosome NN cord-001257-t21l6i3f 228 9 biogenesis biogenesis NN cord-001257-t21l6i3f 228 10 , , , cord-001257-t21l6i3f 228 11 are be VBP cord-001257-t21l6i3f 228 12 likely likely RB cord-001257-t21l6i3f 228 13 involved involve VBN cord-001257-t21l6i3f 228 14 in in IN cord-001257-t21l6i3f 228 15 local local JJ cord-001257-t21l6i3f 228 16 remodeling remodeling NN cord-001257-t21l6i3f 228 17 of of IN cord-001257-t21l6i3f 228 18 large large JJ cord-001257-t21l6i3f 228 19 ribosomal ribosomal JJ cord-001257-t21l6i3f 228 20 RNP RNP NNP cord-001257-t21l6i3f 228 21 structures structure NNS cord-001257-t21l6i3f 228 22 [ [ -LRB- cord-001257-t21l6i3f 228 23 52 52 CD cord-001257-t21l6i3f 228 24 , , , cord-001257-t21l6i3f 228 25 67 67 CD cord-001257-t21l6i3f 228 26 ] ] -RRB- cord-001257-t21l6i3f 228 27 . . . cord-001257-t21l6i3f 229 1 Although although IN cord-001257-t21l6i3f 229 2 eIF4AIII eIF4AIII NNS cord-001257-t21l6i3f 229 3 helicase helicase NN cord-001257-t21l6i3f 229 4 has have VBZ cord-001257-t21l6i3f 229 5 striking strike VBG cord-001257-t21l6i3f 229 6 homology homology NN cord-001257-t21l6i3f 229 7 with with IN cord-001257-t21l6i3f 229 8 eIF4A eIF4A NNS cord-001257-t21l6i3f 229 9 , , , cord-001257-t21l6i3f 229 10 yet yet CC cord-001257-t21l6i3f 229 11 eIF4AIII eIF4AIII JJR cord-001257-t21l6i3f 229 12 is be VBZ cord-001257-t21l6i3f 229 13 functionally functionally RB cord-001257-t21l6i3f 229 14 distinct distinct JJ cord-001257-t21l6i3f 229 15 from from IN cord-001257-t21l6i3f 229 16 eIF4A eIF4A NNS cord-001257-t21l6i3f 229 17 , , , cord-001257-t21l6i3f 229 18 having have VBG cord-001257-t21l6i3f 229 19 no no DT cord-001257-t21l6i3f 229 20 known know VBN cord-001257-t21l6i3f 229 21 role role NN cord-001257-t21l6i3f 229 22 in in IN cord-001257-t21l6i3f 229 23 translation translation NN cord-001257-t21l6i3f 229 24 initiation initiation NN cord-001257-t21l6i3f 229 25 , , , cord-001257-t21l6i3f 229 26 and and CC cord-001257-t21l6i3f 229 27 no no DT cord-001257-t21l6i3f 229 28 interaction interaction NN cord-001257-t21l6i3f 229 29 with with IN cord-001257-t21l6i3f 229 30 ribosome ribosome NN cord-001257-t21l6i3f 229 31 in in IN cord-001257-t21l6i3f 229 32 vitro vitro FW cord-001257-t21l6i3f 229 33 [ [ -LRB- cord-001257-t21l6i3f 229 34 52 52 CD cord-001257-t21l6i3f 229 35 ] ] -RRB- cord-001257-t21l6i3f 229 36 . . . cord-001257-t21l6i3f 230 1 The the DT cord-001257-t21l6i3f 230 2 similar similar JJ cord-001257-t21l6i3f 230 3 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 230 4 helicase helicase NN cord-001257-t21l6i3f 230 5 , , , cord-001257-t21l6i3f 230 6 which which WDT cord-001257-t21l6i3f 230 7 is be VBZ cord-001257-t21l6i3f 230 8 also also RB cord-001257-t21l6i3f 230 9 involved involve VBN cord-001257-t21l6i3f 230 10 in in IN cord-001257-t21l6i3f 230 11 ribosome ribosome NN cord-001257-t21l6i3f 230 12 biogenesis biogenesis NN cord-001257-t21l6i3f 230 13 , , , cord-001257-t21l6i3f 230 14 affects affect VBZ cord-001257-t21l6i3f 230 15 the the DT cord-001257-t21l6i3f 230 16 endonuclease endonuclease NN cord-001257-t21l6i3f 230 17 RNase RNase NNP cord-001257-t21l6i3f 230 18 MRP MRP NNP cord-001257-t21l6i3f 230 19 - - HYPH cord-001257-t21l6i3f 230 20 driven drive VBN cord-001257-t21l6i3f 230 21 cleavage cleavage NN cord-001257-t21l6i3f 230 22 of of IN cord-001257-t21l6i3f 230 23 pre pre JJ cord-001257-t21l6i3f 230 24 - - JJ cord-001257-t21l6i3f 230 25 ribosomal ribosomal JJ cord-001257-t21l6i3f 230 26 RNA RNA NNP cord-001257-t21l6i3f 230 27 [ [ -LRB- cord-001257-t21l6i3f 230 28 51 51 CD cord-001257-t21l6i3f 230 29 ] ] -RRB- cord-001257-t21l6i3f 230 30 . . . cord-001257-t21l6i3f 231 1 It -PRON- PRP cord-001257-t21l6i3f 231 2 is be VBZ cord-001257-t21l6i3f 231 3 currently currently RB cord-001257-t21l6i3f 231 4 not not RB cord-001257-t21l6i3f 231 5 known know VBN cord-001257-t21l6i3f 231 6 how how WRB cord-001257-t21l6i3f 231 7 eIF4AIII eIF4AIII NNS cord-001257-t21l6i3f 231 8 or or CC cord-001257-t21l6i3f 231 9 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 231 10 RNA RNA NNP cord-001257-t21l6i3f 231 11 helicases helicase NNS cord-001257-t21l6i3f 231 12 are be VBP cord-001257-t21l6i3f 231 13 selected select VBN cord-001257-t21l6i3f 231 14 for for IN cord-001257-t21l6i3f 231 15 their -PRON- PRP$ cord-001257-t21l6i3f 231 16 cellular cellular JJ cord-001257-t21l6i3f 231 17 functions function NNS cord-001257-t21l6i3f 231 18 . . . cord-001257-t21l6i3f 232 1 In in IN cord-001257-t21l6i3f 232 2 spite spite NN cord-001257-t21l6i3f 232 3 of of IN cord-001257-t21l6i3f 232 4 their -PRON- PRP$ cord-001257-t21l6i3f 232 5 different different JJ cord-001257-t21l6i3f 232 6 cellular cellular JJ cord-001257-t21l6i3f 232 7 functions function NNS cord-001257-t21l6i3f 232 8 , , , cord-001257-t21l6i3f 232 9 Fal1p Fal1p NNP cord-001257-t21l6i3f 232 10 and and CC cord-001257-t21l6i3f 232 11 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 232 12 helicases helicase NNS cord-001257-t21l6i3f 232 13 play play VBP cord-001257-t21l6i3f 232 14 comparable comparable JJ cord-001257-t21l6i3f 232 15 roles role NNS cord-001257-t21l6i3f 232 16 during during IN cord-001257-t21l6i3f 232 17 TBSV TBSV NNP cord-001257-t21l6i3f 232 18 replication replication NN cord-001257-t21l6i3f 232 19 . . . cord-001257-t21l6i3f 233 1 We -PRON- PRP cord-001257-t21l6i3f 233 2 find find VBP cord-001257-t21l6i3f 233 3 that that IN cord-001257-t21l6i3f 233 4 , , , cord-001257-t21l6i3f 233 5 unlike unlike IN cord-001257-t21l6i3f 233 6 the the DT cord-001257-t21l6i3f 233 7 previously previously RB cord-001257-t21l6i3f 233 8 characterized characterize VBN cord-001257-t21l6i3f 233 9 AtRH20 atrh20 NN cord-001257-t21l6i3f 233 10 / / SYM cord-001257-t21l6i3f 233 11 Ded1p ded1p IN cord-001257-t21l6i3f 233 12 helicases helicase NNS cord-001257-t21l6i3f 233 13 , , , cord-001257-t21l6i3f 233 14 the the DT cord-001257-t21l6i3f 233 15 eIF4AIII eIF4AIII NNS cord-001257-t21l6i3f 233 16 - - HYPH cord-001257-t21l6i3f 233 17 like like JJ cord-001257-t21l6i3f 233 18 RNA RNA NNP cord-001257-t21l6i3f 233 19 helicases helicase NNS cord-001257-t21l6i3f 233 20 ( ( -LRB- cord-001257-t21l6i3f 233 21 i.e. i.e. FW cord-001257-t21l6i3f 233 22 , , , cord-001257-t21l6i3f 233 23 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 233 24 , , , cord-001257-t21l6i3f 233 25 AtRH5 AtRH5 NNP cord-001257-t21l6i3f 233 26 , , , cord-001257-t21l6i3f 233 27 Fal1p Fal1p NNP cord-001257-t21l6i3f 233 28 and and CC cord-001257-t21l6i3f 233 29 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 233 30 ) ) -RRB- cord-001257-t21l6i3f 233 31 bind bind VBP cord-001257-t21l6i3f 233 32 to to IN cord-001257-t21l6i3f 233 33 a a DT cord-001257-t21l6i3f 233 34 different different JJ cord-001257-t21l6i3f 233 35 cis cis NN cord-001257-t21l6i3f 233 36 - - HYPH cord-001257-t21l6i3f 233 37 acting act VBG cord-001257-t21l6i3f 233 38 element element NN cord-001257-t21l6i3f 233 39 , , , cord-001257-t21l6i3f 233 40 the the DT cord-001257-t21l6i3f 233 41 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 233 42 ) ) -RRB- cord-001257-t21l6i3f 233 43 REN REN NNP cord-001257-t21l6i3f 233 44 , , , cord-001257-t21l6i3f 233 45 which which WDT cord-001257-t21l6i3f 233 46 is be VBZ cord-001257-t21l6i3f 233 47 present present JJ cord-001257-t21l6i3f 233 48 at at IN cord-001257-t21l6i3f 233 49 the the DT cord-001257-t21l6i3f 233 50 59 59 CD cord-001257-t21l6i3f 233 51 region region NN cord-001257-t21l6i3f 233 52 of of IN cord-001257-t21l6i3f 233 53 the the DT cord-001257-t21l6i3f 233 54 TBSV TBSV NNP cord-001257-t21l6i3f 234 1 ( ( -LRB- cord-001257-t21l6i3f 234 2 2)RNA 2)rna CD cord-001257-t21l6i3f 235 1 ( ( -LRB- cord-001257-t21l6i3f 235 2 Fig Fig NNP cord-001257-t21l6i3f 235 3 . . . cord-001257-t21l6i3f 235 4 3A 3A NNP cord-001257-t21l6i3f 235 5 ) ) -RRB- cord-001257-t21l6i3f 235 6 . . . cord-001257-t21l6i3f 236 1 Also also RB cord-001257-t21l6i3f 236 2 dissimilar dissimilar JJ cord-001257-t21l6i3f 236 3 with with IN cord-001257-t21l6i3f 236 4 AtRH20 atrh20 NN cord-001257-t21l6i3f 236 5 / / SYM cord-001257-t21l6i3f 236 6 Ded1p ded1p IN cord-001257-t21l6i3f 236 7 helicases helicase NNS cord-001257-t21l6i3f 236 8 is be VBZ cord-001257-t21l6i3f 236 9 the the DT cord-001257-t21l6i3f 236 10 ability ability NN cord-001257-t21l6i3f 236 11 of of IN cord-001257-t21l6i3f 236 12 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 236 13 , , , cord-001257-t21l6i3f 236 14 AtRH5 atrh5 NN cord-001257-t21l6i3f 236 15 and and CC cord-001257-t21l6i3f 236 16 the the DT cord-001257-t21l6i3f 236 17 yeast yeast NN cord-001257-t21l6i3f 236 18 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 236 19 and and CC cord-001257-t21l6i3f 236 20 Fal1p Fal1p NNP cord-001257-t21l6i3f 236 21 to to TO cord-001257-t21l6i3f 236 22 unwind unwind VB cord-001257-t21l6i3f 236 23 the the DT cord-001257-t21l6i3f 236 24 dsRNA dsrna NN cord-001257-t21l6i3f 236 25 structure structure NN cord-001257-t21l6i3f 236 26 within within IN cord-001257-t21l6i3f 236 27 the the DT cord-001257-t21l6i3f 236 28 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 236 29 ) ) -RRB- cord-001257-t21l6i3f 236 30 REN REN NNP cord-001257-t21l6i3f 236 31 ( ( -LRB- cord-001257-t21l6i3f 236 32 Fig Fig NNP cord-001257-t21l6i3f 236 33 . . NNP cord-001257-t21l6i3f 236 34 4 4 CD cord-001257-t21l6i3f 236 35 ) ) -RRB- cord-001257-t21l6i3f 236 36 . . . cord-001257-t21l6i3f 237 1 This this DT cord-001257-t21l6i3f 237 2 unique unique JJ cord-001257-t21l6i3f 237 3 characteristic characteristic NN cord-001257-t21l6i3f 237 4 allows allow VBZ cord-001257-t21l6i3f 237 5 these these DT cord-001257-t21l6i3f 237 6 eIF4AIII eIF4AIII NNS cord-001257-t21l6i3f 237 7 - - HYPH cord-001257-t21l6i3f 237 8 like like JJ cord-001257-t21l6i3f 237 9 RNA RNA NNP cord-001257-t21l6i3f 237 10 helicases helicase NNS cord-001257-t21l6i3f 237 11 to to TO cord-001257-t21l6i3f 237 12 perform perform VB cord-001257-t21l6i3f 237 13 unique unique JJ cord-001257-t21l6i3f 237 14 functions function NNS cord-001257-t21l6i3f 237 15 that that WDT cord-001257-t21l6i3f 237 16 involves involve VBZ cord-001257-t21l6i3f 237 17 the the DT cord-001257-t21l6i3f 237 18 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 237 19 ) ) -RRB- cord-001257-t21l6i3f 237 20 REN REN NNP cord-001257-t21l6i3f 237 21 . . . cord-001257-t21l6i3f 238 1 The the DT cord-001257-t21l6i3f 238 2 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 238 3 and and CC cord-001257-t21l6i3f 238 4 AtRH5 atrh5 NN cord-001257-t21l6i3f 238 5 helicases helicase NNS cord-001257-t21l6i3f 238 6 are be VBP cord-001257-t21l6i3f 238 7 components component NNS cord-001257-t21l6i3f 238 8 of of IN cord-001257-t21l6i3f 238 9 the the DT cord-001257-t21l6i3f 238 10 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 238 11 VRCs VRCs NNP cord-001257-t21l6i3f 238 12 as as IN cord-001257-t21l6i3f 238 13 demonstrated demonstrate VBN cord-001257-t21l6i3f 238 14 by by IN cord-001257-t21l6i3f 238 15 co co JJ cord-001257-t21l6i3f 238 16 - - JJ cord-001257-t21l6i3f 238 17 purification purification JJ cord-001257-t21l6i3f 238 18 experiments experiment NNS cord-001257-t21l6i3f 238 19 ( ( -LRB- cord-001257-t21l6i3f 238 20 Fig Fig NNP cord-001257-t21l6i3f 238 21 . . NNP cord-001257-t21l6i3f 238 22 7 7 CD cord-001257-t21l6i3f 238 23 and and CC cord-001257-t21l6i3f 238 24 S5 S5 NNP cord-001257-t21l6i3f 238 25 ) ) -RRB- cord-001257-t21l6i3f 238 26 . . . cord-001257-t21l6i3f 239 1 In in IN cord-001257-t21l6i3f 239 2 addition addition NN cord-001257-t21l6i3f 239 3 to to IN cord-001257-t21l6i3f 239 4 binding bind VBG cord-001257-t21l6i3f 239 5 to to IN cord-001257-t21l6i3f 239 6 the the DT cord-001257-t21l6i3f 239 7 viral viral JJ cord-001257-t21l6i3f 239 8 RNAs rna NNS cord-001257-t21l6i3f 239 9 , , , cord-001257-t21l6i3f 239 10 these these DT cord-001257-t21l6i3f 239 11 eIF4AIII eIF4AIII NNS cord-001257-t21l6i3f 239 12 - - HYPH cord-001257-t21l6i3f 239 13 like like JJ cord-001257-t21l6i3f 239 14 RNA RNA NNP cord-001257-t21l6i3f 239 15 helicases helicase NNS cord-001257-t21l6i3f 239 16 also also RB cord-001257-t21l6i3f 239 17 bind bind VBP cord-001257-t21l6i3f 239 18 to to IN cord-001257-t21l6i3f 239 19 the the DT cord-001257-t21l6i3f 239 20 p33 p33 CD cord-001257-t21l6i3f 239 21 / / SYM cord-001257-t21l6i3f 239 22 p92 p92 NN cord-001257-t21l6i3f 239 23 replication replication NN cord-001257-t21l6i3f 239 24 proteins protein NNS cord-001257-t21l6i3f 239 25 , , , cord-001257-t21l6i3f 239 26 likely likely RB cord-001257-t21l6i3f 239 27 facilitating facilitate VBG cord-001257-t21l6i3f 239 28 the the DT cord-001257-t21l6i3f 239 29 recruitment recruitment NN cord-001257-t21l6i3f 239 30 of of IN cord-001257-t21l6i3f 239 31 these these DT cord-001257-t21l6i3f 239 32 cellular cellular JJ cord-001257-t21l6i3f 239 33 helicases helicase NNS cord-001257-t21l6i3f 239 34 into into IN cord-001257-t21l6i3f 239 35 VRCs VRCs NNP cord-001257-t21l6i3f 239 36 . . . cord-001257-t21l6i3f 240 1 It -PRON- PRP cord-001257-t21l6i3f 240 2 is be VBZ cord-001257-t21l6i3f 240 3 possible possible JJ cord-001257-t21l6i3f 240 4 that that IN cord-001257-t21l6i3f 240 5 additional additional JJ cord-001257-t21l6i3f 240 6 members member NNS cord-001257-t21l6i3f 240 7 of of IN cord-001257-t21l6i3f 240 8 the the DT cord-001257-t21l6i3f 240 9 large large JJ cord-001257-t21l6i3f 240 10 helicase helicase NN cord-001257-t21l6i3f 240 11 family family NN cord-001257-t21l6i3f 240 12 could could MD cord-001257-t21l6i3f 240 13 perform perform VB cord-001257-t21l6i3f 240 14 a a DT cord-001257-t21l6i3f 240 15 similar similar JJ cord-001257-t21l6i3f 240 16 function function NN cord-001257-t21l6i3f 240 17 to to IN cord-001257-t21l6i3f 240 18 eIF4AIII eIF4AIII NNS cord-001257-t21l6i3f 240 19 - - HYPH cord-001257-t21l6i3f 240 20 like like JJ cord-001257-t21l6i3f 240 21 RNA RNA NNP cord-001257-t21l6i3f 240 22 helicases helicase NNS cord-001257-t21l6i3f 240 23 during during IN cord-001257-t21l6i3f 240 24 TBSV TBSV NNP cord-001257-t21l6i3f 240 25 replication replication NN cord-001257-t21l6i3f 240 26 . . . cord-001257-t21l6i3f 241 1 Indeed indeed RB cord-001257-t21l6i3f 241 2 , , , cord-001257-t21l6i3f 241 3 39 39 CD cord-001257-t21l6i3f 241 4 RNA RNA NNP cord-001257-t21l6i3f 241 5 helicases helicase NNS cord-001257-t21l6i3f 241 6 are be VBP cord-001257-t21l6i3f 241 7 present present JJ cord-001257-t21l6i3f 241 8 in in IN cord-001257-t21l6i3f 241 9 yeast yeast NN cord-001257-t21l6i3f 241 10 and and CC cord-001257-t21l6i3f 241 11 110 110 CD cord-001257-t21l6i3f 241 12 - - SYM cord-001257-t21l6i3f 241 13 160 160 CD cord-001257-t21l6i3f 241 14 RNA RNA NNP cord-001257-t21l6i3f 241 15 helicases helicase NNS cord-001257-t21l6i3f 241 16 are be VBP cord-001257-t21l6i3f 241 17 described describe VBN cord-001257-t21l6i3f 241 18 in in IN cord-001257-t21l6i3f 241 19 plants plant NNS cord-001257-t21l6i3f 241 20 [ [ -LRB- cord-001257-t21l6i3f 241 21 44 44 CD cord-001257-t21l6i3f 241 22 , , , cord-001257-t21l6i3f 241 23 68 68 CD cord-001257-t21l6i3f 241 24 ] ] -RRB- cord-001257-t21l6i3f 241 25 , , , cord-001257-t21l6i3f 241 26 indicating indicate VBG cord-001257-t21l6i3f 241 27 that that IN cord-001257-t21l6i3f 241 28 additional additional JJ cord-001257-t21l6i3f 241 29 members member NNS cord-001257-t21l6i3f 241 30 of of IN cord-001257-t21l6i3f 241 31 this this DT cord-001257-t21l6i3f 241 32 large large JJ cord-001257-t21l6i3f 241 33 protein protein NN cord-001257-t21l6i3f 241 34 family family NN cord-001257-t21l6i3f 241 35 could could MD cord-001257-t21l6i3f 241 36 also also RB cord-001257-t21l6i3f 241 37 be be VB cord-001257-t21l6i3f 241 38 involved involve VBN cord-001257-t21l6i3f 241 39 in in IN cord-001257-t21l6i3f 241 40 TBSV TBSV NNP cord-001257-t21l6i3f 241 41 replication replication NN cord-001257-t21l6i3f 241 42 . . . cord-001257-t21l6i3f 242 1 One one CD cord-001257-t21l6i3f 242 2 of of IN cord-001257-t21l6i3f 242 3 the the DT cord-001257-t21l6i3f 242 4 hallmark hallmark NN cord-001257-t21l6i3f 242 5 features feature NNS cord-001257-t21l6i3f 242 6 of of IN cord-001257-t21l6i3f 242 7 ( ( -LRB- cord-001257-t21l6i3f 242 8 + + CC cord-001257-t21l6i3f 242 9 ) ) -RRB- cord-001257-t21l6i3f 242 10 RNA rna NN cord-001257-t21l6i3f 242 11 virus virus NN cord-001257-t21l6i3f 242 12 replication replication NN cord-001257-t21l6i3f 242 13 is be VBZ cord-001257-t21l6i3f 242 14 the the DT cord-001257-t21l6i3f 242 15 asymmetric asymmetric JJ cord-001257-t21l6i3f 242 16 nature nature NN cord-001257-t21l6i3f 242 17 of of IN cord-001257-t21l6i3f 242 18 RNA RNA NNP cord-001257-t21l6i3f 242 19 synthesis synthesis NN cord-001257-t21l6i3f 242 20 [ [ -LRB- cord-001257-t21l6i3f 242 21 12 12 CD cord-001257-t21l6i3f 242 22 , , , cord-001257-t21l6i3f 242 23 [ [ -LRB- cord-001257-t21l6i3f 242 24 69 69 CD cord-001257-t21l6i3f 242 25 ] ] -RRB- cord-001257-t21l6i3f 242 26 [ [ -LRB- cord-001257-t21l6i3f 242 27 70 70 CD cord-001257-t21l6i3f 242 28 ] ] -RRB- cord-001257-t21l6i3f 242 29 [ [ -LRB- cord-001257-t21l6i3f 242 30 71 71 CD cord-001257-t21l6i3f 242 31 ] ] -RRB- cord-001257-t21l6i3f 242 32 . . . cord-001257-t21l6i3f 243 1 The the DT cord-001257-t21l6i3f 243 2 replication replication NN cord-001257-t21l6i3f 243 3 process process NN cord-001257-t21l6i3f 243 4 leads lead VBZ cord-001257-t21l6i3f 243 5 to to IN cord-001257-t21l6i3f 243 6 the the DT cord-001257-t21l6i3f 243 7 production production NN cord-001257-t21l6i3f 243 8 of of IN cord-001257-t21l6i3f 243 9 abundant abundant JJ cord-001257-t21l6i3f 243 10 ( ( -LRB- cord-001257-t21l6i3f 243 11 + + NNS cord-001257-t21l6i3f 243 12 ) ) -RRB- cord-001257-t21l6i3f 244 1 RNA rna NN cord-001257-t21l6i3f 244 2 progeny progeny NN cord-001257-t21l6i3f 244 3 , , , cord-001257-t21l6i3f 244 4 while while IN cord-001257-t21l6i3f 244 5 the the DT cord-001257-t21l6i3f 244 6 ( ( -LRB- cord-001257-t21l6i3f 244 7 2)RNA 2)rna CD cord-001257-t21l6i3f 244 8 templates template NNS cord-001257-t21l6i3f 244 9 are be VBP cord-001257-t21l6i3f 244 10 likely likely RB cord-001257-t21l6i3f 244 11 sequestered sequester VBN cord-001257-t21l6i3f 244 12 in in IN cord-001257-t21l6i3f 244 13 dsRNA dsrna JJ cord-001257-t21l6i3f 244 14 forms form NNS cord-001257-t21l6i3f 244 15 within within IN cord-001257-t21l6i3f 244 16 the the DT cord-001257-t21l6i3f 244 17 VRCs VRCs NNP cord-001257-t21l6i3f 244 18 . . . cord-001257-t21l6i3f 245 1 The the DT cord-001257-t21l6i3f 245 2 presented present VBN cord-001257-t21l6i3f 245 3 in in IN cord-001257-t21l6i3f 245 4 vitro vitro FW cord-001257-t21l6i3f 245 5 data datum NNS cord-001257-t21l6i3f 245 6 based base VBN cord-001257-t21l6i3f 245 7 on on IN cord-001257-t21l6i3f 245 8 the the DT cord-001257-t21l6i3f 245 9 solubilized solubilized JJ cord-001257-t21l6i3f 245 10 / / SYM cord-001257-t21l6i3f 245 11 purified purify VBN cord-001257-t21l6i3f 245 12 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 245 13 replicase replicase NN cord-001257-t21l6i3f 245 14 and and CC cord-001257-t21l6i3f 245 15 the the DT cord-001257-t21l6i3f 245 16 CFE CFE NNP cord-001257-t21l6i3f 245 17 assay assay NN cord-001257-t21l6i3f 245 18 containing contain VBG cord-001257-t21l6i3f 245 19 the the DT cord-001257-t21l6i3f 245 20 membrane membrane NN cord-001257-t21l6i3f 245 21 - - HYPH cord-001257-t21l6i3f 245 22 bound bind VBN cord-001257-t21l6i3f 245 23 VRC VRC NNP cord-001257-t21l6i3f 245 24 indicate indicate VBP cord-001257-t21l6i3f 245 25 that that IN cord-001257-t21l6i3f 245 26 the the DT cord-001257-t21l6i3f 245 27 eIF4AIIIlike eIF4AIIIlike NNP cord-001257-t21l6i3f 245 28 RNA RNA NNP cord-001257-t21l6i3f 245 29 helicases helicase NNS cord-001257-t21l6i3f 245 30 can can MD cord-001257-t21l6i3f 245 31 mainly mainly RB cord-001257-t21l6i3f 245 32 stimulate stimulate VB cord-001257-t21l6i3f 245 33 TBSV TBSV NNP cord-001257-t21l6i3f 245 34 ( ( -LRB- cord-001257-t21l6i3f 245 35 + + NN cord-001257-t21l6i3f 245 36 ) ) -RRB- cord-001257-t21l6i3f 246 1 -strand -strand CD cord-001257-t21l6i3f 246 2 synthesis synthesis NN cord-001257-t21l6i3f 246 3 , , , cord-001257-t21l6i3f 246 4 while while IN cord-001257-t21l6i3f 246 5 their -PRON- PRP$ cord-001257-t21l6i3f 246 6 effects effect NNS cord-001257-t21l6i3f 246 7 on on IN cord-001257-t21l6i3f 246 8 ( ( -LRB- cord-001257-t21l6i3f 246 9 2)RNA 2)rna CD cord-001257-t21l6i3f 246 10 synthesis synthesis NN cord-001257-t21l6i3f 246 11 have have VBP cord-001257-t21l6i3f 246 12 not not RB cord-001257-t21l6i3f 246 13 been be VBN cord-001257-t21l6i3f 246 14 observed observe VBN cord-001257-t21l6i3f 246 15 ( ( -LRB- cord-001257-t21l6i3f 246 16 not not RB cord-001257-t21l6i3f 246 17 shown show VBN cord-001257-t21l6i3f 246 18 ) ) -RRB- cord-001257-t21l6i3f 246 19 . . . cord-001257-t21l6i3f 247 1 The the DT cord-001257-t21l6i3f 247 2 recombinant recombinant JJ cord-001257-t21l6i3f 247 3 eIF4AIII eIF4AIII NNS cord-001257-t21l6i3f 247 4 - - HYPH cord-001257-t21l6i3f 247 5 like like JJ cord-001257-t21l6i3f 247 6 RNA RNA NNP cord-001257-t21l6i3f 247 7 helicases helicase NNS cord-001257-t21l6i3f 247 8 enhanced enhance VBN cord-001257-t21l6i3f 247 9 ( ( -LRB- cord-001257-t21l6i3f 247 10 + + CC cord-001257-t21l6i3f 248 1 ) ) -RRB- cord-001257-t21l6i3f 248 2 strand strand NN cord-001257-t21l6i3f 248 3 synthesis synthesis NN cord-001257-t21l6i3f 248 4 by by IN cord-001257-t21l6i3f 248 5 the the DT cord-001257-t21l6i3f 248 6 purified purify VBN cord-001257-t21l6i3f 248 7 recombinant recombinant JJ cord-001257-t21l6i3f 248 8 tombusvirus tombusvirus NN cord-001257-t21l6i3f 248 9 replicase replicase NN cord-001257-t21l6i3f 248 10 , , , cord-001257-t21l6i3f 248 11 it -PRON- PRP cord-001257-t21l6i3f 248 12 is be VBZ cord-001257-t21l6i3f 248 13 possible possible JJ cord-001257-t21l6i3f 248 14 that that IN cord-001257-t21l6i3f 248 15 these these DT cord-001257-t21l6i3f 248 16 helicases helicase NNS cord-001257-t21l6i3f 248 17 directly directly RB cord-001257-t21l6i3f 248 18 affect affect VB cord-001257-t21l6i3f 248 19 TBSV TBSV NNP cord-001257-t21l6i3f 248 20 RNA RNA NNP cord-001257-t21l6i3f 248 21 synthesis synthesis NN cord-001257-t21l6i3f 248 22 via via IN cord-001257-t21l6i3f 248 23 affecting affect VBG cord-001257-t21l6i3f 248 24 the the DT cord-001257-t21l6i3f 248 25 structure structure NN cord-001257-t21l6i3f 248 26 of of IN cord-001257-t21l6i3f 248 27 the the DT cord-001257-t21l6i3f 248 28 RNA RNA NNP cord-001257-t21l6i3f 248 29 templates template NNS cord-001257-t21l6i3f 248 30 , , , cord-001257-t21l6i3f 248 31 including include VBG cord-001257-t21l6i3f 248 32 the the DT cord-001257-t21l6i3f 248 33 RIII(2)REN riii(2)ren NN cord-001257-t21l6i3f 248 34 . . . cord-001257-t21l6i3f 249 1 However however RB cord-001257-t21l6i3f 249 2 , , , cord-001257-t21l6i3f 249 3 we -PRON- PRP cord-001257-t21l6i3f 249 4 can can MD cord-001257-t21l6i3f 249 5 not not RB cord-001257-t21l6i3f 249 6 fully fully RB cord-001257-t21l6i3f 249 7 exclude exclude VB cord-001257-t21l6i3f 249 8 that that IN cord-001257-t21l6i3f 249 9 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 249 10 , , , cord-001257-t21l6i3f 249 11 AtRH5 atrh5 NN cord-001257-t21l6i3f 249 12 and and CC cord-001257-t21l6i3f 249 13 the the DT cord-001257-t21l6i3f 249 14 yeast yeast NN cord-001257-t21l6i3f 249 15 Fal1p Fal1p NNP cord-001257-t21l6i3f 249 16 and and CC cord-001257-t21l6i3f 249 17 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 249 18 helicases helicase NNS cord-001257-t21l6i3f 249 19 could could MD cord-001257-t21l6i3f 249 20 also also RB cord-001257-t21l6i3f 249 21 affect affect VB cord-001257-t21l6i3f 249 22 the the DT cord-001257-t21l6i3f 249 23 activity activity NN cord-001257-t21l6i3f 249 24 of of IN cord-001257-t21l6i3f 249 25 the the DT cord-001257-t21l6i3f 249 26 VRC VRC NNP cord-001257-t21l6i3f 249 27 due due IN cord-001257-t21l6i3f 249 28 to to IN cord-001257-t21l6i3f 249 29 their -PRON- PRP$ cord-001257-t21l6i3f 249 30 interactions interaction NNS cord-001257-t21l6i3f 249 31 with with IN cord-001257-t21l6i3f 249 32 p33 p33 CD cord-001257-t21l6i3f 249 33 and and CC cord-001257-t21l6i3f 249 34 p92 p92 NN cord-001257-t21l6i3f 249 35 ( ( -LRB- cord-001257-t21l6i3f 249 36 Fig Fig NNP cord-001257-t21l6i3f 249 37 . . NNP cord-001257-t21l6i3f 249 38 7 7 CD cord-001257-t21l6i3f 249 39 ) ) -RRB- cord-001257-t21l6i3f 249 40 . . . cord-001257-t21l6i3f 250 1 Overall overall RB cord-001257-t21l6i3f 250 2 , , , cord-001257-t21l6i3f 250 3 the the DT cord-001257-t21l6i3f 250 4 recruitment recruitment NN cord-001257-t21l6i3f 250 5 of of IN cord-001257-t21l6i3f 250 6 eIF4AIIIlike eIF4AIIIlike NNP cord-001257-t21l6i3f 250 7 DEAD DEAD NNP cord-001257-t21l6i3f 250 8 - - HYPH cord-001257-t21l6i3f 250 9 box box NN cord-001257-t21l6i3f 250 10 helicases helicase NNS cord-001257-t21l6i3f 250 11 for for IN cord-001257-t21l6i3f 250 12 replication replication NN cord-001257-t21l6i3f 250 13 of of IN cord-001257-t21l6i3f 250 14 a a DT cord-001257-t21l6i3f 250 15 small small JJ cord-001257-t21l6i3f 250 16 RNA RNA NNP cord-001257-t21l6i3f 250 17 virus virus NN cord-001257-t21l6i3f 250 18 is be VBZ cord-001257-t21l6i3f 250 19 remarkable remarkable JJ cord-001257-t21l6i3f 250 20 , , , cord-001257-t21l6i3f 250 21 and and CC cord-001257-t21l6i3f 250 22 we -PRON- PRP cord-001257-t21l6i3f 250 23 suggest suggest VBP cord-001257-t21l6i3f 250 24 that that IN cord-001257-t21l6i3f 250 25 small small JJ cord-001257-t21l6i3f 250 26 ( ( -LRB- cord-001257-t21l6i3f 250 27 + + JJ cord-001257-t21l6i3f 250 28 ) ) -RRB- cord-001257-t21l6i3f 250 29 RNA RNA NNP cord-001257-t21l6i3f 250 30 viruses virus NNS cord-001257-t21l6i3f 250 31 likely likely RB cord-001257-t21l6i3f 250 32 co co JJ cord-001257-t21l6i3f 250 33 - - VB cord-001257-t21l6i3f 250 34 opt opt VB cord-001257-t21l6i3f 250 35 two two CD cord-001257-t21l6i3f 250 36 or or CC cord-001257-t21l6i3f 250 37 more more RBR cord-001257-t21l6i3f 250 38 different different JJ cord-001257-t21l6i3f 250 39 host host NN cord-001257-t21l6i3f 250 40 helicases helicase NNS cord-001257-t21l6i3f 250 41 that that WDT cord-001257-t21l6i3f 250 42 interact interact VBP cord-001257-t21l6i3f 250 43 with with IN cord-001257-t21l6i3f 250 44 different different JJ cord-001257-t21l6i3f 250 45 cisacting cisacte VBG cord-001257-t21l6i3f 250 46 elements element NNS cord-001257-t21l6i3f 250 47 in in IN cord-001257-t21l6i3f 250 48 the the DT cord-001257-t21l6i3f 250 49 viral viral JJ cord-001257-t21l6i3f 250 50 RNA RNA NNP cord-001257-t21l6i3f 250 51 to to TO cord-001257-t21l6i3f 250 52 aid aid VB cord-001257-t21l6i3f 250 53 viral viral JJ cord-001257-t21l6i3f 250 54 replication replication NN cord-001257-t21l6i3f 250 55 . . . cord-001257-t21l6i3f 251 1 Based base VBN cord-001257-t21l6i3f 251 2 on on IN cord-001257-t21l6i3f 251 3 their -PRON- PRP$ cord-001257-t21l6i3f 251 4 RNA RNA NNP cord-001257-t21l6i3f 251 5 binding binding NN cord-001257-t21l6i3f 251 6 features feature NNS cord-001257-t21l6i3f 251 7 and and CC cord-001257-t21l6i3f 251 8 their -PRON- PRP$ cord-001257-t21l6i3f 251 9 abilities ability NNS cord-001257-t21l6i3f 251 10 to to TO cord-001257-t21l6i3f 251 11 unwind unwind VB cord-001257-t21l6i3f 251 12 dsRNA dsrna NN cord-001257-t21l6i3f 251 13 regions region NNS cord-001257-t21l6i3f 251 14 only only RB cord-001257-t21l6i3f 251 15 locally locally RB cord-001257-t21l6i3f 251 16 , , , cord-001257-t21l6i3f 251 17 we -PRON- PRP cord-001257-t21l6i3f 251 18 propose propose VBP cord-001257-t21l6i3f 251 19 that that IN cord-001257-t21l6i3f 251 20 the the DT cord-001257-t21l6i3f 251 21 helicase helicase NN cord-001257-t21l6i3f 251 22 functions function NNS cord-001257-t21l6i3f 251 23 of of IN cord-001257-t21l6i3f 251 24 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 251 25 , , , cord-001257-t21l6i3f 251 26 AtRH5 atrh5 NN cord-001257-t21l6i3f 251 27 and and CC cord-001257-t21l6i3f 251 28 the the DT cord-001257-t21l6i3f 251 29 yeast yeast NN cord-001257-t21l6i3f 251 30 Fal1p Fal1p NNP cord-001257-t21l6i3f 251 31 and and CC cord-001257-t21l6i3f 251 32 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 251 33 are be VBP cord-001257-t21l6i3f 251 34 likely likely RB cord-001257-t21l6i3f 251 35 important important JJ cord-001257-t21l6i3f 251 36 for for IN cord-001257-t21l6i3f 251 37 unwinding unwind VBG cord-001257-t21l6i3f 251 38 of of IN cord-001257-t21l6i3f 251 39 the the DT cord-001257-t21l6i3f 251 40 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 251 41 ) ) -RRB- cord-001257-t21l6i3f 252 1 REN REN NNP cord-001257-t21l6i3f 252 2 region region NN cord-001257-t21l6i3f 252 3 in in IN cord-001257-t21l6i3f 252 4 the the DT cord-001257-t21l6i3f 252 5 dsRNA dsrna NN cord-001257-t21l6i3f 252 6 structure structure NN cord-001257-t21l6i3f 252 7 formed form VBN cord-001257-t21l6i3f 252 8 within within IN cord-001257-t21l6i3f 252 9 the the DT cord-001257-t21l6i3f 252 10 VRCs vrcs NN cord-001257-t21l6i3f 252 11 during during IN cord-001257-t21l6i3f 252 12 TBSV TBSV NNP cord-001257-t21l6i3f 252 13 replication replication NN cord-001257-t21l6i3f 252 14 . . . cord-001257-t21l6i3f 253 1 Why why WRB cord-001257-t21l6i3f 253 2 is be VBZ cord-001257-t21l6i3f 253 3 local local JJ cord-001257-t21l6i3f 253 4 unwinding unwinding NN cord-001257-t21l6i3f 253 5 of of IN cord-001257-t21l6i3f 253 6 dsRNA dsrna NN cord-001257-t21l6i3f 253 7 within within IN cord-001257-t21l6i3f 253 8 the the DT cord-001257-t21l6i3f 253 9 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 253 10 ) ) -RRB- cord-001257-t21l6i3f 254 1 REN REN NNP cord-001257-t21l6i3f 254 2 stimulatory stimulatory JJ cord-001257-t21l6i3f 254 3 for for IN cord-001257-t21l6i3f 254 4 replication replication NN cord-001257-t21l6i3f 254 5 ? ? . cord-001257-t21l6i3f 255 1 We -PRON- PRP cord-001257-t21l6i3f 255 2 suggest suggest VBP cord-001257-t21l6i3f 255 3 that that IN cord-001257-t21l6i3f 255 4 the the DT cord-001257-t21l6i3f 255 5 locally locally RB cord-001257-t21l6i3f 255 6 opened open VBN cord-001257-t21l6i3f 255 7 RIII RIII NNP cord-001257-t21l6i3f 255 8 in in IN cord-001257-t21l6i3f 255 9 the the DT cord-001257-t21l6i3f 255 10 dsRNA dsrna NN cord-001257-t21l6i3f 255 11 form form NN cord-001257-t21l6i3f 255 12 might may MD cord-001257-t21l6i3f 255 13 allow allow VB cord-001257-t21l6i3f 255 14 the the DT cord-001257-t21l6i3f 255 15 bridge bridge NN cord-001257-t21l6i3f 255 16 sequence sequence NN cord-001257-t21l6i3f 255 17 within within IN cord-001257-t21l6i3f 255 18 the the DT cord-001257-t21l6i3f 255 19 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 255 20 ) ) -RRB- cord-001257-t21l6i3f 255 21 REN REN NNP cord-001257-t21l6i3f 255 22 to to TO cord-001257-t21l6i3f 255 23 participate participate VB cord-001257-t21l6i3f 255 24 in in IN cord-001257-t21l6i3f 255 25 a a DT cord-001257-t21l6i3f 255 26 long long JJ cord-001257-t21l6i3f 255 27 - - HYPH cord-001257-t21l6i3f 255 28 range range NN cord-001257-t21l6i3f 255 29 basepairing basepairing NN cord-001257-t21l6i3f 255 30 with with IN cord-001257-t21l6i3f 255 31 the the DT cord-001257-t21l6i3f 255 32 39end 39end NN cord-001257-t21l6i3f 255 33 of of IN cord-001257-t21l6i3f 255 34 the the DT cord-001257-t21l6i3f 255 35 ( ( -LRB- cord-001257-t21l6i3f 255 36 2)RNA 2)rna CD cord-001257-t21l6i3f 255 37 , , , cord-001257-t21l6i3f 255 38 thus thus RB cord-001257-t21l6i3f 255 39 bringing bring VBG cord-001257-t21l6i3f 255 40 the the DT cord-001257-t21l6i3f 255 41 59 59 CD cord-001257-t21l6i3f 255 42 and and CC cord-001257-t21l6i3f 255 43 39 39 CD cord-001257-t21l6i3f 255 44 terminal terminal NN cord-001257-t21l6i3f 255 45 sequences sequence NNS cord-001257-t21l6i3f 255 46 of of IN cord-001257-t21l6i3f 255 47 the the DT cord-001257-t21l6i3f 255 48 ( ( -LRB- cord-001257-t21l6i3f 255 49 2)RNA 2)rna CD cord-001257-t21l6i3f 255 50 in in IN cord-001257-t21l6i3f 255 51 close close JJ cord-001257-t21l6i3f 255 52 vicinity vicinity NN cord-001257-t21l6i3f 255 53 ( ( -LRB- cord-001257-t21l6i3f 255 54 Fig fig NN cord-001257-t21l6i3f 255 55 . . . cord-001257-t21l6i3f 255 56 9 9 CD cord-001257-t21l6i3f 255 57 ) ) -RRB- cord-001257-t21l6i3f 255 58 . . . cord-001257-t21l6i3f 256 1 This this DT cord-001257-t21l6i3f 256 2 could could MD cord-001257-t21l6i3f 256 3 facilitate facilitate VB cord-001257-t21l6i3f 256 4 ( ( -LRB- cord-001257-t21l6i3f 256 5 + + NNS cord-001257-t21l6i3f 256 6 ) ) -RRB- cord-001257-t21l6i3f 257 1 -strand -strand CD cord-001257-t21l6i3f 257 2 synthesis synthesis NNP cord-001257-t21l6i3f 257 3 and and CC cord-001257-t21l6i3f 257 4 the the DT cord-001257-t21l6i3f 257 5 reutilization reutilization NN cord-001257-t21l6i3f 257 6 of of IN cord-001257-t21l6i3f 257 7 the the DT cord-001257-t21l6i3f 257 8 viral viral JJ cord-001257-t21l6i3f 257 9 replicases replicase NNS cord-001257-t21l6i3f 257 10 ( ( -LRB- cord-001257-t21l6i3f 257 11 VRCs VRCs NNP cord-001257-t21l6i3f 257 12 ) ) -RRB- cord-001257-t21l6i3f 257 13 for for IN cord-001257-t21l6i3f 257 14 multiple multiple JJ cord-001257-t21l6i3f 257 15 rounds round NNS cord-001257-t21l6i3f 257 16 ( ( -LRB- cord-001257-t21l6i3f 257 17 as as IN cord-001257-t21l6i3f 257 18 discussed discuss VBN cord-001257-t21l6i3f 257 19 below below RB cord-001257-t21l6i3f 257 20 ) ) -RRB- cord-001257-t21l6i3f 257 21 . . . cord-001257-t21l6i3f 258 1 However however RB cord-001257-t21l6i3f 258 2 , , , cord-001257-t21l6i3f 258 3 the the DT cord-001257-t21l6i3f 258 4 long long JJ cord-001257-t21l6i3f 258 5 - - HYPH cord-001257-t21l6i3f 258 6 distance distance NN cord-001257-t21l6i3f 258 7 base base NN cord-001257-t21l6i3f 258 8 pairing pair VBG cord-001257-t21l6i3f 258 9 between between IN cord-001257-t21l6i3f 258 10 the the DT cord-001257-t21l6i3f 258 11 ' ' `` cord-001257-t21l6i3f 258 12 ' ' `` cord-001257-t21l6i3f 258 13 bridge bridge NN cord-001257-t21l6i3f 258 14 ' ' '' cord-001257-t21l6i3f 258 15 ' ' '' cord-001257-t21l6i3f 258 16 in in IN cord-001257-t21l6i3f 258 17 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 258 18 ) ) -RRB- cord-001257-t21l6i3f 259 1 REN REN NNP cord-001257-t21l6i3f 259 2 and and CC cord-001257-t21l6i3f 259 3 the the DT cord-001257-t21l6i3f 259 4 cPR cPR NNP cord-001257-t21l6i3f 259 5 promoter promoter NN cord-001257-t21l6i3f 259 6 in in IN cord-001257-t21l6i3f 259 7 RI(2 RI(2 NNP cord-001257-t21l6i3f 259 8 ) ) -RRB- cord-001257-t21l6i3f 259 9 , , , cord-001257-t21l6i3f 259 10 both both DT cord-001257-t21l6i3f 259 11 of of IN cord-001257-t21l6i3f 259 12 which which WDT cord-001257-t21l6i3f 259 13 are be VBP cord-001257-t21l6i3f 259 14 buried bury VBN cord-001257-t21l6i3f 259 15 in in IN cord-001257-t21l6i3f 259 16 the the DT cord-001257-t21l6i3f 259 17 dsRNA dsrna NN cord-001257-t21l6i3f 259 18 structure structure NN cord-001257-t21l6i3f 259 19 , , , cord-001257-t21l6i3f 259 20 should should MD cord-001257-t21l6i3f 259 21 also also RB cord-001257-t21l6i3f 259 22 depend depend VB cord-001257-t21l6i3f 259 23 on on IN cord-001257-t21l6i3f 259 24 opening open VBG cord-001257-t21l6i3f 259 25 the the DT cord-001257-t21l6i3f 259 26 dsRNA dsrna NN cord-001257-t21l6i3f 259 27 form form NN cord-001257-t21l6i3f 259 28 within within IN cord-001257-t21l6i3f 259 29 RI(2 RI(2 NNP cord-001257-t21l6i3f 259 30 ) ) -RRB- cord-001257-t21l6i3f 259 31 . . . cord-001257-t21l6i3f 260 1 This this DT cord-001257-t21l6i3f 260 2 function function NN cord-001257-t21l6i3f 260 3 is be VBZ cord-001257-t21l6i3f 260 4 unlikely unlikely RB cord-001257-t21l6i3f 260 5 performed perform VBN cord-001257-t21l6i3f 260 6 by by IN cord-001257-t21l6i3f 260 7 eIF4AIII eIF4AIII NNS cord-001257-t21l6i3f 260 8 - - HYPH cord-001257-t21l6i3f 260 9 like like JJ cord-001257-t21l6i3f 260 10 RNA RNA NNP cord-001257-t21l6i3f 260 11 helicases helicase NNS cord-001257-t21l6i3f 260 12 . . . cord-001257-t21l6i3f 261 1 Instead instead RB cord-001257-t21l6i3f 261 2 , , , cord-001257-t21l6i3f 261 3 we -PRON- PRP cord-001257-t21l6i3f 261 4 have have VBP cord-001257-t21l6i3f 261 5 previously previously RB cord-001257-t21l6i3f 261 6 demonstrated demonstrate VBN cord-001257-t21l6i3f 261 7 that that IN cord-001257-t21l6i3f 261 8 the the DT cord-001257-t21l6i3f 261 9 subverted subvert VBN cord-001257-t21l6i3f 261 10 DDX3-like DDX3-like NNP cord-001257-t21l6i3f 261 11 AtRH20 atrh20 NN cord-001257-t21l6i3f 261 12 / / SYM cord-001257-t21l6i3f 261 13 Ded1p ded1p IN cord-001257-t21l6i3f 261 14 helicases helicase NNS cord-001257-t21l6i3f 261 15 could could MD cord-001257-t21l6i3f 261 16 open open VB cord-001257-t21l6i3f 261 17 up up RP cord-001257-t21l6i3f 261 18 the the DT cord-001257-t21l6i3f 261 19 dsRNA dsrna NN cord-001257-t21l6i3f 261 20 structure structure NN cord-001257-t21l6i3f 261 21 within within IN cord-001257-t21l6i3f 261 22 the the DT cord-001257-t21l6i3f 261 23 RI(2 RI(2 NNP cord-001257-t21l6i3f 261 24 ) ) -RRB- cord-001257-t21l6i3f 261 25 sequence sequence NN cord-001257-t21l6i3f 261 26 [ [ -LRB- cord-001257-t21l6i3f 261 27 30 30 CD cord-001257-t21l6i3f 261 28 ] ] -RRB- cord-001257-t21l6i3f 261 29 . . . cord-001257-t21l6i3f 262 1 In in IN cord-001257-t21l6i3f 262 2 summary summary NN cord-001257-t21l6i3f 262 3 , , , cord-001257-t21l6i3f 262 4 based base VBN cord-001257-t21l6i3f 262 5 on on IN cord-001257-t21l6i3f 262 6 this this DT cord-001257-t21l6i3f 262 7 and and CC cord-001257-t21l6i3f 262 8 previous previous JJ cord-001257-t21l6i3f 262 9 publications publication NNS cord-001257-t21l6i3f 262 10 [ [ -LRB- cord-001257-t21l6i3f 262 11 21 21 CD cord-001257-t21l6i3f 262 12 , , , cord-001257-t21l6i3f 262 13 30 30 CD cord-001257-t21l6i3f 262 14 , , , cord-001257-t21l6i3f 262 15 49 49 CD cord-001257-t21l6i3f 262 16 ] ] -RRB- cord-001257-t21l6i3f 262 17 , , , cord-001257-t21l6i3f 262 18 the the DT cord-001257-t21l6i3f 262 19 emerging emerge VBG cord-001257-t21l6i3f 262 20 picture picture NN cord-001257-t21l6i3f 262 21 with with IN cord-001257-t21l6i3f 262 22 TBSV TBSV NNP cord-001257-t21l6i3f 262 23 is be VBZ cord-001257-t21l6i3f 262 24 that that IN cord-001257-t21l6i3f 262 25 this this DT cord-001257-t21l6i3f 262 26 virus virus NN cord-001257-t21l6i3f 262 27 utilizes utilize VBZ cord-001257-t21l6i3f 262 28 co co VBN cord-001257-t21l6i3f 262 29 - - JJ cord-001257-t21l6i3f 262 30 opted opt VBN cord-001257-t21l6i3f 262 31 RNA RNA NNP cord-001257-t21l6i3f 262 32 - - HYPH cord-001257-t21l6i3f 262 33 binding bind VBG cord-001257-t21l6i3f 262 34 host host NN cord-001257-t21l6i3f 262 35 proteins protein NNS cord-001257-t21l6i3f 262 36 to to TO cord-001257-t21l6i3f 262 37 regulate regulate VB cord-001257-t21l6i3f 262 38 asymmetric asymmetric JJ cord-001257-t21l6i3f 262 39 viral viral JJ cord-001257-t21l6i3f 262 40 RNA RNA NNP cord-001257-t21l6i3f 262 41 replication replication NN cord-001257-t21l6i3f 262 42 . . . cord-001257-t21l6i3f 263 1 The the DT cord-001257-t21l6i3f 263 2 recruited recruit VBN cord-001257-t21l6i3f 263 3 host host NN cord-001257-t21l6i3f 263 4 proteins protein NNS cord-001257-t21l6i3f 263 5 are be VBP cord-001257-t21l6i3f 263 6 needed need VBN cord-001257-t21l6i3f 263 7 for for IN cord-001257-t21l6i3f 263 8 specific specific JJ cord-001257-t21l6i3f 263 9 interactions interaction NNS cord-001257-t21l6i3f 263 10 with with IN cord-001257-t21l6i3f 263 11 various various JJ cord-001257-t21l6i3f 263 12 cis cis NNP cord-001257-t21l6i3f 263 13 - - HYPH cord-001257-t21l6i3f 263 14 acting act VBG cord-001257-t21l6i3f 263 15 sequences sequence NNS cord-001257-t21l6i3f 263 16 in in IN cord-001257-t21l6i3f 263 17 the the DT cord-001257-t21l6i3f 263 18 viral viral NN cord-001257-t21l6i3f 263 19 ( ( -LRB- cord-001257-t21l6i3f 263 20 2)RNA 2)rna CD cord-001257-t21l6i3f 263 21 because because IN cord-001257-t21l6i3f 263 22 the the DT cord-001257-t21l6i3f 263 23 viral viral JJ cord-001257-t21l6i3f 263 24 p33 p33 CD cord-001257-t21l6i3f 263 25 / / SYM cord-001257-t21l6i3f 263 26 p92 p92 NN cord-001257-t21l6i3f 263 27 replication replication NN cord-001257-t21l6i3f 263 28 proteins protein NNS cord-001257-t21l6i3f 263 29 bind bind VBP cord-001257-t21l6i3f 263 30 to to IN cord-001257-t21l6i3f 263 31 TBSV TBSV NNP cord-001257-t21l6i3f 263 32 ( ( -LRB- cord-001257-t21l6i3f 263 33 2 2 CD cord-001257-t21l6i3f 263 34 ) ) -RRB- cord-001257-t21l6i3f 264 1 RNA RNA NNP cord-001257-t21l6i3f 264 2 nonspecifically nonspecifically RB cord-001257-t21l6i3f 264 3 [ [ -LRB- cord-001257-t21l6i3f 264 4 72 72 CD cord-001257-t21l6i3f 264 5 ] ] -RRB- cord-001257-t21l6i3f 264 6 . . . cord-001257-t21l6i3f 265 1 We -PRON- PRP cord-001257-t21l6i3f 265 2 propose propose VBP cord-001257-t21l6i3f 265 3 that that IN cord-001257-t21l6i3f 265 4 , , , cord-001257-t21l6i3f 265 5 first first RB cord-001257-t21l6i3f 265 6 , , , cord-001257-t21l6i3f 265 7 the the DT cord-001257-t21l6i3f 265 8 recruited recruit VBN cord-001257-t21l6i3f 265 9 eIF4AIII eIF4AIII NNS cord-001257-t21l6i3f 265 10 - - HYPH cord-001257-t21l6i3f 265 11 like like JJ cord-001257-t21l6i3f 265 12 RNA RNA NNP cord-001257-t21l6i3f 265 13 helicase helicase NN cord-001257-t21l6i3f 265 14 proteins protein NNS cord-001257-t21l6i3f 265 15 bind bind VBP cord-001257-t21l6i3f 265 16 to to IN cord-001257-t21l6i3f 265 17 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 265 18 ) ) -RRB- cord-001257-t21l6i3f 266 1 REN REN NNP cord-001257-t21l6i3f 266 2 , , , cord-001257-t21l6i3f 266 3 while while IN cord-001257-t21l6i3f 266 4 the the DT cord-001257-t21l6i3f 266 5 DDX3-like DDX3-like NNP cord-001257-t21l6i3f 266 6 AtRH20 AtRH20 NNP cord-001257-t21l6i3f 266 7 / / SYM cord-001257-t21l6i3f 266 8 Ded1p ded1p IN cord-001257-t21l6i3f 266 9 helicases helicase NNS cord-001257-t21l6i3f 266 10 bind bind VBP cord-001257-t21l6i3f 266 11 to to IN cord-001257-t21l6i3f 266 12 RI(2 RI(2 NNP cord-001257-t21l6i3f 266 13 ) ) -RRB- cord-001257-t21l6i3f 266 14 sequence sequence NN cord-001257-t21l6i3f 266 15 . . . cord-001257-t21l6i3f 267 1 The the DT cord-001257-t21l6i3f 267 2 interactions interaction NNS cord-001257-t21l6i3f 267 3 of of IN cord-001257-t21l6i3f 267 4 two two CD cord-001257-t21l6i3f 267 5 groups group NNS cord-001257-t21l6i3f 267 6 of of IN cord-001257-t21l6i3f 267 7 helicases helicase NNS cord-001257-t21l6i3f 267 8 with with IN cord-001257-t21l6i3f 267 9 the the DT cord-001257-t21l6i3f 267 10 viral viral JJ cord-001257-t21l6i3f 267 11 dsRNA dsrna NN cord-001257-t21l6i3f 267 12 likely likely RB cord-001257-t21l6i3f 267 13 opens open VBZ cord-001257-t21l6i3f 267 14 up up RP cord-001257-t21l6i3f 267 15 the the DT cord-001257-t21l6i3f 267 16 59 59 CD cord-001257-t21l6i3f 267 17 proximal proximal JJ cord-001257-t21l6i3f 267 18 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 267 19 ) ) -RRB- cord-001257-t21l6i3f 268 1 REN REN NNP cord-001257-t21l6i3f 268 2 and and CC cord-001257-t21l6i3f 268 3 the the DT cord-001257-t21l6i3f 268 4 39 39 CD cord-001257-t21l6i3f 268 5 terminal terminal NN cord-001257-t21l6i3f 268 6 promoter promoter NN cord-001257-t21l6i3f 268 7 region region NN cord-001257-t21l6i3f 268 8 from from IN cord-001257-t21l6i3f 268 9 the the DT cord-001257-t21l6i3f 268 10 dsRNA dsrna NN cord-001257-t21l6i3f 268 11 structure structure NN cord-001257-t21l6i3f 268 12 present present JJ cord-001257-t21l6i3f 268 13 in in IN cord-001257-t21l6i3f 268 14 the the DT cord-001257-t21l6i3f 268 15 VRCs vrcs NN cord-001257-t21l6i3f 268 16 . . . cord-001257-t21l6i3f 269 1 Then then RB cord-001257-t21l6i3f 269 2 , , , cord-001257-t21l6i3f 269 3 long long JJ cord-001257-t21l6i3f 269 4 - - HYPH cord-001257-t21l6i3f 269 5 distance distance NN cord-001257-t21l6i3f 269 6 RNA RNA NNP cord-001257-t21l6i3f 269 7 - - HYPH cord-001257-t21l6i3f 269 8 RNA RNA NNP cord-001257-t21l6i3f 269 9 interaction interaction NN cord-001257-t21l6i3f 269 10 between between IN cord-001257-t21l6i3f 269 11 the the DT cord-001257-t21l6i3f 269 12 bridge bridge NN cord-001257-t21l6i3f 269 13 sequence sequence NN cord-001257-t21l6i3f 269 14 in in IN cord-001257-t21l6i3f 269 15 the the DT cord-001257-t21l6i3f 269 16 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 269 17 ) ) -RRB- cord-001257-t21l6i3f 269 18 REN REN NNP cord-001257-t21l6i3f 269 19 and and CC cord-001257-t21l6i3f 269 20 the the DT cord-001257-t21l6i3f 269 21 39 39 CD cord-001257-t21l6i3f 269 22 terminal terminal NN cord-001257-t21l6i3f 269 23 sequence sequence NN cord-001257-t21l6i3f 269 24 [ [ -LRB- cord-001257-t21l6i3f 269 25 57 57 CD cord-001257-t21l6i3f 269 26 ] ] -RRB- cord-001257-t21l6i3f 269 27 could could MD cord-001257-t21l6i3f 269 28 ' ' `` cord-001257-t21l6i3f 269 29 ' ' `` cord-001257-t21l6i3f 269 30 circularize circularize VB cord-001257-t21l6i3f 269 31 ' ' '' cord-001257-t21l6i3f 269 32 ' ' '' cord-001257-t21l6i3f 269 33 the the DT cord-001257-t21l6i3f 269 34 ( ( -LRB- cord-001257-t21l6i3f 269 35 2)RNA 2)rna CD cord-001257-t21l6i3f 269 36 template template NN cord-001257-t21l6i3f 269 37 and and CC cord-001257-t21l6i3f 269 38 bring bring VB cord-001257-t21l6i3f 269 39 the the DT cord-001257-t21l6i3f 269 40 p92 p92 NN cord-001257-t21l6i3f 269 41 RdRp RdRp NNP cord-001257-t21l6i3f 269 42 protein protein NN cord-001257-t21l6i3f 269 43 from from IN cord-001257-t21l6i3f 269 44 the the DT cord-001257-t21l6i3f 269 45 59 59 CD cord-001257-t21l6i3f 269 46 end end NN cord-001257-t21l6i3f 269 47 back back RB cord-001257-t21l6i3f 269 48 to to IN cord-001257-t21l6i3f 269 49 the the DT cord-001257-t21l6i3f 269 50 39 39 CD cord-001257-t21l6i3f 269 51 end end NN cord-001257-t21l6i3f 269 52 for for IN cord-001257-t21l6i3f 269 53 a a DT cord-001257-t21l6i3f 269 54 new new JJ cord-001257-t21l6i3f 269 55 round round NN cord-001257-t21l6i3f 269 56 of of IN cord-001257-t21l6i3f 269 57 ( ( -LRB- cord-001257-t21l6i3f 269 58 + + CC cord-001257-t21l6i3f 269 59 ) ) -RRB- cord-001257-t21l6i3f 269 60 strand strand NN cord-001257-t21l6i3f 269 61 synthesis synthesis NN cord-001257-t21l6i3f 269 62 ( ( -LRB- cord-001257-t21l6i3f 269 63 Fig Fig NNP cord-001257-t21l6i3f 269 64 . . . cord-001257-t21l6i3f 269 65 9 9 CD cord-001257-t21l6i3f 269 66 ) ) -RRB- cord-001257-t21l6i3f 269 67 . . . cord-001257-t21l6i3f 270 1 As as IN cord-001257-t21l6i3f 270 2 proposed propose VBN cord-001257-t21l6i3f 270 3 earlier early RBR cord-001257-t21l6i3f 270 4 [ [ -LRB- cord-001257-t21l6i3f 270 5 30 30 CD cord-001257-t21l6i3f 270 6 , , , cord-001257-t21l6i3f 270 7 49 49 CD cord-001257-t21l6i3f 270 8 ] ] -RRB- cord-001257-t21l6i3f 270 9 , , , cord-001257-t21l6i3f 270 10 an an DT cord-001257-t21l6i3f 270 11 additional additional JJ cord-001257-t21l6i3f 270 12 function function NN cord-001257-t21l6i3f 270 13 of of IN cord-001257-t21l6i3f 270 14 AtRH20 atrh20 NN cord-001257-t21l6i3f 270 15 / / SYM cord-001257-t21l6i3f 270 16 Ded1p ded1p NN cord-001257-t21l6i3f 270 17 is be VBZ cord-001257-t21l6i3f 270 18 to to TO cord-001257-t21l6i3f 270 19 further further RB cord-001257-t21l6i3f 270 20 unwind unwind VB cord-001257-t21l6i3f 270 21 local local JJ cord-001257-t21l6i3f 270 22 secondary secondary JJ cord-001257-t21l6i3f 270 23 structure structure NN cord-001257-t21l6i3f 270 24 within within IN cord-001257-t21l6i3f 270 25 RI(2 RI(2 NNP cord-001257-t21l6i3f 270 26 ) ) -RRB- cord-001257-t21l6i3f 270 27 to to TO cord-001257-t21l6i3f 270 28 promote promote VB cord-001257-t21l6i3f 270 29 the the DT cord-001257-t21l6i3f 270 30 association association NN cord-001257-t21l6i3f 270 31 of of IN cord-001257-t21l6i3f 270 32 the the DT cord-001257-t21l6i3f 270 33 cellular cellular JJ cord-001257-t21l6i3f 270 34 GAPDH GAPDH NNP cord-001257-t21l6i3f 270 35 with with IN cord-001257-t21l6i3f 270 36 an an DT cord-001257-t21l6i3f 270 37 AU au JJ cord-001257-t21l6i3f 270 38 - - HYPH cord-001257-t21l6i3f 270 39 rich rich JJ cord-001257-t21l6i3f 270 40 internal internal JJ cord-001257-t21l6i3f 270 41 site site NN cord-001257-t21l6i3f 270 42 and and CC cord-001257-t21l6i3f 270 43 proper proper JJ cord-001257-t21l6i3f 270 44 positioning positioning NN cord-001257-t21l6i3f 270 45 of of IN cord-001257-t21l6i3f 270 46 the the DT cord-001257-t21l6i3f 270 47 GAPDH GAPDH NNP cord-001257-t21l6i3f 270 48 - - HYPH cord-001257-t21l6i3f 270 49 p92 p92 NN cord-001257-t21l6i3f 270 50 RdRp rdrp NN cord-001257-t21l6i3f 270 51 complex complex NN cord-001257-t21l6i3f 270 52 [ [ -LRB- cord-001257-t21l6i3f 270 53 73 73 CD cord-001257-t21l6i3f 270 54 ] ] -RRB- cord-001257-t21l6i3f 270 55 over over IN cord-001257-t21l6i3f 270 56 the the DT cord-001257-t21l6i3f 270 57 ( ( -LRB- cord-001257-t21l6i3f 270 58 + + NN cord-001257-t21l6i3f 270 59 ) ) -RRB- cord-001257-t21l6i3f 270 60 -strand -strand CD cord-001257-t21l6i3f 270 61 initiation initiation NN cord-001257-t21l6i3f 270 62 promoter promoter NN cord-001257-t21l6i3f 270 63 , , , cord-001257-t21l6i3f 270 64 leading lead VBG cord-001257-t21l6i3f 270 65 to to IN cord-001257-t21l6i3f 270 66 robust robust JJ cord-001257-t21l6i3f 270 67 ( ( -LRB- cord-001257-t21l6i3f 270 68 + + JJ cord-001257-t21l6i3f 270 69 ) ) -RRB- cord-001257-t21l6i3f 270 70 RNA rna NN cord-001257-t21l6i3f 270 71 synthesis synthesis NN cord-001257-t21l6i3f 270 72 . . . cord-001257-t21l6i3f 271 1 Therefore therefore RB cord-001257-t21l6i3f 271 2 , , , cord-001257-t21l6i3f 271 3 we -PRON- PRP cord-001257-t21l6i3f 271 4 propose propose VBP cord-001257-t21l6i3f 271 5 that that IN cord-001257-t21l6i3f 271 6 the the DT cord-001257-t21l6i3f 271 7 synergistic synergistic JJ cord-001257-t21l6i3f 271 8 effect effect NN cord-001257-t21l6i3f 271 9 between between IN cord-001257-t21l6i3f 271 10 the the DT cord-001257-t21l6i3f 271 11 two two CD cord-001257-t21l6i3f 271 12 groups group NNS cord-001257-t21l6i3f 271 13 of of IN cord-001257-t21l6i3f 271 14 subverted subvert VBN cord-001257-t21l6i3f 271 15 helicases helicase NNS cord-001257-t21l6i3f 271 16 , , , cord-001257-t21l6i3f 271 17 host host NN cord-001257-t21l6i3f 271 18 GAPDH GAPDH NNP cord-001257-t21l6i3f 271 19 and and CC cord-001257-t21l6i3f 271 20 the the DT cord-001257-t21l6i3f 271 21 viral viral JJ cord-001257-t21l6i3f 271 22 p92 p92 NN cord-001257-t21l6i3f 271 23 pol pol NN cord-001257-t21l6i3f 271 24 might may MD cord-001257-t21l6i3f 271 25 promote promote VB cord-001257-t21l6i3f 271 26 efficient efficient JJ cord-001257-t21l6i3f 271 27 recycling recycling NN cord-001257-t21l6i3f 271 28 of of IN cord-001257-t21l6i3f 271 29 the the DT cord-001257-t21l6i3f 271 30 viral viral JJ cord-001257-t21l6i3f 271 31 RdRp RdRp NNP cord-001257-t21l6i3f 271 32 , , , cord-001257-t21l6i3f 271 33 resulting result VBG cord-001257-t21l6i3f 271 34 in in IN cord-001257-t21l6i3f 271 35 multiple multiple JJ cord-001257-t21l6i3f 271 36 rounds round NNS cord-001257-t21l6i3f 271 37 of of IN cord-001257-t21l6i3f 271 38 ( ( -LRB- cord-001257-t21l6i3f 271 39 + + CC cord-001257-t21l6i3f 271 40 ) ) -RRB- cord-001257-t21l6i3f 271 41 RNA rna NN cord-001257-t21l6i3f 271 42 synthesis synthesis NN cord-001257-t21l6i3f 271 43 on on IN cord-001257-t21l6i3f 271 44 the the DT cord-001257-t21l6i3f 271 45 same same JJ cord-001257-t21l6i3f 271 46 dsRNA dsrna NN cord-001257-t21l6i3f 271 47 template template NN cord-001257-t21l6i3f 271 48 ( ( -LRB- cord-001257-t21l6i3f 271 49 Fig Fig NNP cord-001257-t21l6i3f 271 50 . . . cord-001257-t21l6i3f 271 51 9 9 CD cord-001257-t21l6i3f 271 52 ) ) -RRB- cord-001257-t21l6i3f 271 53 . . . cord-001257-t21l6i3f 272 1 This this DT cord-001257-t21l6i3f 272 2 strategy strategy NN cord-001257-t21l6i3f 272 3 could could MD cord-001257-t21l6i3f 272 4 be be VB cord-001257-t21l6i3f 272 5 beneficial beneficial JJ cord-001257-t21l6i3f 272 6 for for IN cord-001257-t21l6i3f 272 7 the the DT cord-001257-t21l6i3f 272 8 virus virus NN cord-001257-t21l6i3f 272 9 by by IN cord-001257-t21l6i3f 272 10 allowing allow VBG cord-001257-t21l6i3f 272 11 asymmetric asymmetric JJ cord-001257-t21l6i3f 272 12 RNA RNA NNP cord-001257-t21l6i3f 272 13 synthesis synthesis NN cord-001257-t21l6i3f 272 14 on on IN cord-001257-t21l6i3f 272 15 dsRNA dsrna NN cord-001257-t21l6i3f 272 16 templates template NNS cord-001257-t21l6i3f 272 17 , , , cord-001257-t21l6i3f 272 18 thus thus RB cord-001257-t21l6i3f 272 19 leading lead VBG cord-001257-t21l6i3f 272 20 to to IN cord-001257-t21l6i3f 272 21 excess excess JJ cord-001257-t21l6i3f 272 22 amount amount NN cord-001257-t21l6i3f 272 23 of of IN cord-001257-t21l6i3f 272 24 progeny progeny NN cord-001257-t21l6i3f 272 25 ( ( -LRB- cord-001257-t21l6i3f 272 26 + + NNS cord-001257-t21l6i3f 272 27 ) ) -RRB- cord-001257-t21l6i3f 272 28 RNAs rna NNS cord-001257-t21l6i3f 272 29 . . . cord-001257-t21l6i3f 273 1 It -PRON- PRP cord-001257-t21l6i3f 273 2 is be VBZ cord-001257-t21l6i3f 273 3 currently currently RB cord-001257-t21l6i3f 273 4 not not RB cord-001257-t21l6i3f 273 5 known know VBN cord-001257-t21l6i3f 273 6 if if IN cord-001257-t21l6i3f 273 7 other other JJ cord-001257-t21l6i3f 273 8 viruses virus NNS cord-001257-t21l6i3f 273 9 might may MD cord-001257-t21l6i3f 273 10 also also RB cord-001257-t21l6i3f 273 11 use use VB cord-001257-t21l6i3f 273 12 two two CD cord-001257-t21l6i3f 273 13 different different JJ cord-001257-t21l6i3f 273 14 groups group NNS cord-001257-t21l6i3f 273 15 of of IN cord-001257-t21l6i3f 273 16 cellular cellular JJ cord-001257-t21l6i3f 273 17 helicases helicase NNS cord-001257-t21l6i3f 273 18 to to TO cord-001257-t21l6i3f 273 19 aid aid VB cord-001257-t21l6i3f 273 20 their -PRON- PRP$ cord-001257-t21l6i3f 273 21 replication replication NN cord-001257-t21l6i3f 273 22 . . . cord-001257-t21l6i3f 274 1 However however RB cord-001257-t21l6i3f 274 2 , , , cord-001257-t21l6i3f 274 3 HIV HIV NNP cord-001257-t21l6i3f 274 4 retrovirus retrovirus NN cord-001257-t21l6i3f 274 5 , , , cord-001257-t21l6i3f 274 6 which which WDT cord-001257-t21l6i3f 274 7 also also RB cord-001257-t21l6i3f 274 8 lacks lack VBZ cord-001257-t21l6i3f 274 9 viral viral RB cord-001257-t21l6i3f 274 10 - - HYPH cord-001257-t21l6i3f 274 11 coded code VBN cord-001257-t21l6i3f 274 12 helicases helicase NNS cord-001257-t21l6i3f 274 13 , , , cord-001257-t21l6i3f 274 14 has have VBZ cord-001257-t21l6i3f 274 15 been be VBN cord-001257-t21l6i3f 274 16 shown show VBN cord-001257-t21l6i3f 274 17 to to TO cord-001257-t21l6i3f 274 18 recruit recruit VB cord-001257-t21l6i3f 274 19 several several JJ cord-001257-t21l6i3f 274 20 cellular cellular JJ cord-001257-t21l6i3f 274 21 helicases helicase NNS cord-001257-t21l6i3f 274 22 , , , cord-001257-t21l6i3f 274 23 including include VBG cord-001257-t21l6i3f 274 24 DDX3 DDX3 NNP cord-001257-t21l6i3f 274 25 , , , cord-001257-t21l6i3f 274 26 for for IN cord-001257-t21l6i3f 274 27 various various JJ cord-001257-t21l6i3f 274 28 steps step NNS cord-001257-t21l6i3f 274 29 of of IN cord-001257-t21l6i3f 274 30 its -PRON- PRP$ cord-001257-t21l6i3f 274 31 infection infection NN cord-001257-t21l6i3f 274 32 cycle cycle NN cord-001257-t21l6i3f 274 33 [ [ -LRB- cord-001257-t21l6i3f 274 34 74 74 CD cord-001257-t21l6i3f 274 35 ] ] -RRB- cord-001257-t21l6i3f 275 1 [ [ -LRB- cord-001257-t21l6i3f 275 2 75 75 CD cord-001257-t21l6i3f 275 3 ] ] -RRB- cord-001257-t21l6i3f 275 4 [ [ -LRB- cord-001257-t21l6i3f 275 5 76 76 CD cord-001257-t21l6i3f 275 6 ] ] -RRB- cord-001257-t21l6i3f 275 7 . . . cord-001257-t21l6i3f 276 1 In in IN cord-001257-t21l6i3f 276 2 addition addition NN cord-001257-t21l6i3f 276 3 , , , cord-001257-t21l6i3f 276 4 host host NN cord-001257-t21l6i3f 276 5 DEAD DEAD NNP cord-001257-t21l6i3f 276 6 - - HYPH cord-001257-t21l6i3f 276 7 box box NN cord-001257-t21l6i3f 276 8 helicases helicase NNS cord-001257-t21l6i3f 276 9 have have VBP cord-001257-t21l6i3f 276 10 been be VBN cord-001257-t21l6i3f 276 11 shown show VBN cord-001257-t21l6i3f 276 12 to to TO cord-001257-t21l6i3f 276 13 affect affect VB cord-001257-t21l6i3f 276 14 virus virus NN cord-001257-t21l6i3f 276 15 infections infection NNS cord-001257-t21l6i3f 276 16 , , , cord-001257-t21l6i3f 276 17 including include VBG cord-001257-t21l6i3f 276 18 translation translation NN cord-001257-t21l6i3f 276 19 of of IN cord-001257-t21l6i3f 276 20 viral viral JJ cord-001257-t21l6i3f 276 21 proteins protein NNS cord-001257-t21l6i3f 276 22 [ [ -LRB- cord-001257-t21l6i3f 276 23 77 77 CD cord-001257-t21l6i3f 276 24 ] ] -RRB- cord-001257-t21l6i3f 276 25 [ [ -LRB- cord-001257-t21l6i3f 276 26 78 78 CD cord-001257-t21l6i3f 276 27 ] ] -RRB- cord-001257-t21l6i3f 276 28 [ [ -LRB- cord-001257-t21l6i3f 276 29 79 79 CD cord-001257-t21l6i3f 276 30 ] ] -RRB- cord-001257-t21l6i3f 276 31 ; ; : cord-001257-t21l6i3f 276 32 viral viral JJ cord-001257-t21l6i3f 276 33 RNA RNA NNP cord-001257-t21l6i3f 276 34 replication replication NN cord-001257-t21l6i3f 276 35 [ [ -LRB- cord-001257-t21l6i3f 276 36 43 43 CD cord-001257-t21l6i3f 276 37 , , , cord-001257-t21l6i3f 276 38 [ [ -LRB- cord-001257-t21l6i3f 276 39 80 80 CD cord-001257-t21l6i3f 276 40 ] ] -RRB- cord-001257-t21l6i3f 276 41 [ [ -LRB- cord-001257-t21l6i3f 276 42 81 81 CD cord-001257-t21l6i3f 276 43 ] ] -RRB- cord-001257-t21l6i3f 276 44 [ [ -LRB- cord-001257-t21l6i3f 276 45 82 82 CD cord-001257-t21l6i3f 276 46 ] ] -RRB- cord-001257-t21l6i3f 276 47 [ [ -LRB- cord-001257-t21l6i3f 276 48 83 83 CD cord-001257-t21l6i3f 276 49 ] ] -RRB- cord-001257-t21l6i3f 276 50 ; ; : cord-001257-t21l6i3f 276 51 subgenomic subgenomic NNP cord-001257-t21l6i3f 276 52 RNA RNA NNP cord-001257-t21l6i3f 276 53 synthesis synthesis NN cord-001257-t21l6i3f 276 54 [ [ -LRB- cord-001257-t21l6i3f 276 55 84 84 CD cord-001257-t21l6i3f 276 56 ] ] -RRB- cord-001257-t21l6i3f 276 57 ; ; : cord-001257-t21l6i3f 276 58 reverse reverse VB cord-001257-t21l6i3f 276 59 transcription transcription NN cord-001257-t21l6i3f 276 60 [ [ -LRB- cord-001257-t21l6i3f 276 61 85 85 CD cord-001257-t21l6i3f 276 62 ] ] -RRB- cord-001257-t21l6i3f 276 63 ; ; : cord-001257-t21l6i3f 276 64 virus virus NN cord-001257-t21l6i3f 276 65 assembly assembly NN cord-001257-t21l6i3f 276 66 [ [ -LRB- cord-001257-t21l6i3f 276 67 86 86 CD cord-001257-t21l6i3f 276 68 ] ] -RRB- cord-001257-t21l6i3f 276 69 ; ; : cord-001257-t21l6i3f 276 70 virus virus NN cord-001257-t21l6i3f 276 71 - - HYPH cord-001257-t21l6i3f 276 72 mediated mediate VBN cord-001257-t21l6i3f 276 73 regulation regulation NN cord-001257-t21l6i3f 276 74 of of IN cord-001257-t21l6i3f 276 75 host host NN cord-001257-t21l6i3f 276 76 gene gene NN cord-001257-t21l6i3f 276 77 transcription transcription NN cord-001257-t21l6i3f 276 78 [ [ -LRB- cord-001257-t21l6i3f 276 79 87 87 CD cord-001257-t21l6i3f 276 80 ] ] -RRB- cord-001257-t21l6i3f 276 81 , , , cord-001257-t21l6i3f 276 82 and and CC cord-001257-t21l6i3f 276 83 the the DT cord-001257-t21l6i3f 276 84 activity activity NN cord-001257-t21l6i3f 276 85 of of IN cord-001257-t21l6i3f 276 86 many many JJ cord-001257-t21l6i3f 276 87 anti anti JJ cord-001257-t21l6i3f 276 88 - - JJ cord-001257-t21l6i3f 276 89 viral viral JJ cord-001257-t21l6i3f 276 90 proteins protein NNS cord-001257-t21l6i3f 276 91 [ [ -LRB- cord-001257-t21l6i3f 276 92 88 88 CD cord-001257-t21l6i3f 276 93 ] ] -RRB- cord-001257-t21l6i3f 276 94 [ [ -LRB- cord-001257-t21l6i3f 276 95 89 89 CD cord-001257-t21l6i3f 276 96 ] ] -RRB- cord-001257-t21l6i3f 276 97 [ [ -LRB- cord-001257-t21l6i3f 276 98 90 90 CD cord-001257-t21l6i3f 276 99 ] ] -RRB- cord-001257-t21l6i3f 276 100 . . . cord-001257-t21l6i3f 277 1 Therefore therefore RB cord-001257-t21l6i3f 277 2 , , , cord-001257-t21l6i3f 277 3 the the DT cord-001257-t21l6i3f 277 4 emerging emerge VBG cord-001257-t21l6i3f 277 5 picture picture NN cord-001257-t21l6i3f 277 6 is be VBZ cord-001257-t21l6i3f 277 7 that that IN cord-001257-t21l6i3f 277 8 RNA RNA NNP cord-001257-t21l6i3f 277 9 viruses virus NNS cord-001257-t21l6i3f 277 10 subvert subvert VBP cord-001257-t21l6i3f 277 11 multiple multiple JJ cord-001257-t21l6i3f 277 12 members member NNS cord-001257-t21l6i3f 277 13 of of IN cord-001257-t21l6i3f 277 14 the the DT cord-001257-t21l6i3f 277 15 cellular cellular JJ cord-001257-t21l6i3f 277 16 RNA RNA NNP cord-001257-t21l6i3f 277 17 helicase helicase NN cord-001257-t21l6i3f 277 18 family family NN cord-001257-t21l6i3f 277 19 during during IN cord-001257-t21l6i3f 277 20 infections infection NNS cord-001257-t21l6i3f 277 21 . . . cord-001257-t21l6i3f 278 1 Saccharomyces saccharomyce NNS cord-001257-t21l6i3f 278 2 cerevisiae cerevisiae NNS cord-001257-t21l6i3f 278 3 strain strain VBP cord-001257-t21l6i3f 278 4 BY4741 by4741 NN cord-001257-t21l6i3f 278 5 , , , cord-001257-t21l6i3f 278 6 Ddbp3 Ddbp3 NNP cord-001257-t21l6i3f 278 7 ( ( -LRB- cord-001257-t21l6i3f 278 8 YKO YKO NNP cord-001257-t21l6i3f 278 9 library library NN cord-001257-t21l6i3f 278 10 ) ) -RRB- cord-001257-t21l6i3f 278 11 and and CC cord-001257-t21l6i3f 278 12 TET::Fal1 TET::Fal1 NNS cord-001257-t21l6i3f 278 13 yeast yeast NN cord-001257-t21l6i3f 278 14 strain strain NN cord-001257-t21l6i3f 278 15 ( ( -LRB- cord-001257-t21l6i3f 278 16 yTHC yTHC NNP cord-001257-t21l6i3f 278 17 library library NN cord-001257-t21l6i3f 278 18 ) ) -RRB- cord-001257-t21l6i3f 278 19 , , , cord-001257-t21l6i3f 278 20 were be VBD cord-001257-t21l6i3f 278 21 obtained obtain VBN cord-001257-t21l6i3f 278 22 from from IN cord-001257-t21l6i3f 278 23 Open Open NNP cord-001257-t21l6i3f 278 24 Biosystems Biosystems NNP cord-001257-t21l6i3f 278 25 ( ( -LRB- cord-001257-t21l6i3f 278 26 Huntsville Huntsville NNP cord-001257-t21l6i3f 278 27 , , , cord-001257-t21l6i3f 278 28 AL AL NNP cord-001257-t21l6i3f 278 29 , , , cord-001257-t21l6i3f 278 30 USA USA NNP cord-001257-t21l6i3f 278 31 ) ) -RRB- cord-001257-t21l6i3f 278 32 . . . cord-001257-t21l6i3f 279 1 Ts Ts NNP cord-001257-t21l6i3f 279 2 - - HYPH cord-001257-t21l6i3f 279 3 Fal1 Fal1 NNP cord-001257-t21l6i3f 279 4 yeast yeast NN cord-001257-t21l6i3f 279 5 strain strain NN cord-001257-t21l6i3f 279 6 was be VBD cord-001257-t21l6i3f 279 7 from from IN cord-001257-t21l6i3f 279 8 a a DT cord-001257-t21l6i3f 279 9 yeast yeast NN cord-001257-t21l6i3f 279 10 ts t MD cord-001257-t21l6i3f 279 11 strain strain NN cord-001257-t21l6i3f 279 12 collection collection NN cord-001257-t21l6i3f 279 13 [ [ -LRB- cord-001257-t21l6i3f 279 14 91 91 CD cord-001257-t21l6i3f 279 15 ] ] -RRB- cord-001257-t21l6i3f 279 16 . . . cord-001257-t21l6i3f 280 1 Yeast yeast NN cord-001257-t21l6i3f 280 2 strain strain NN cord-001257-t21l6i3f 281 1 NMY51 NMY51 NNP cord-001257-t21l6i3f 281 2 was be VBD cord-001257-t21l6i3f 281 3 obtained obtain VBN cord-001257-t21l6i3f 281 4 from from IN cord-001257-t21l6i3f 281 5 Dualsystems Dualsystems NNP cord-001257-t21l6i3f 281 6 . . . cord-001257-t21l6i3f 282 1 Ddbp3 Ddbp3 NNP cord-001257-t21l6i3f 283 1 /ts /ts LS cord-001257-t21l6i3f 283 2 - - : cord-001257-t21l6i3f 283 3 Fal1 Fal1 NNP cord-001257-t21l6i3f 283 4 yeast yeast NN cord-001257-t21l6i3f 283 5 strain strain NN cord-001257-t21l6i3f 283 6 was be VBD cord-001257-t21l6i3f 283 7 generated generate VBN cord-001257-t21l6i3f 283 8 as as IN cord-001257-t21l6i3f 283 9 follows follow VBZ cord-001257-t21l6i3f 283 10 : : : cord-001257-t21l6i3f 283 11 plasmid plasmid NN cord-001257-t21l6i3f 283 12 pYM-14 pym-14 CD cord-001257-t21l6i3f 283 13 ( ( -LRB- cord-001257-t21l6i3f 283 14 EUROSCARF EUROSCARF NNP cord-001257-t21l6i3f 283 15 ) ) -RRB- cord-001257-t21l6i3f 283 16 [ [ -LRB- cord-001257-t21l6i3f 283 17 92 92 CD cord-001257-t21l6i3f 283 18 ] ] -RRB- cord-001257-t21l6i3f 283 19 was be VBD cord-001257-t21l6i3f 283 20 used use VBN cord-001257-t21l6i3f 283 21 for for IN cord-001257-t21l6i3f 283 22 PCR PCR NNP cord-001257-t21l6i3f 283 23 with with IN cord-001257-t21l6i3f 283 24 primers primer NNS cord-001257-t21l6i3f 283 25 # # $ cord-001257-t21l6i3f 283 26 5011 5011 CD cord-001257-t21l6i3f 283 27 and and CC cord-001257-t21l6i3f 283 28 # # $ cord-001257-t21l6i3f 283 29 5012 5012 CD cord-001257-t21l6i3f 283 30 ( ( -LRB- cord-001257-t21l6i3f 283 31 Table Table NNP cord-001257-t21l6i3f 283 32 S1 S1 NNP cord-001257-t21l6i3f 283 33 ) ) -RRB- cord-001257-t21l6i3f 283 34 to to TO cord-001257-t21l6i3f 283 35 amplify amplify VB cord-001257-t21l6i3f 283 36 the the DT cord-001257-t21l6i3f 283 37 Dbp3 Dbp3 NNP cord-001257-t21l6i3f 283 38 deletion deletion NN cord-001257-t21l6i3f 283 39 cassette cassette NN cord-001257-t21l6i3f 283 40 . . . cord-001257-t21l6i3f 284 1 Ts Ts NNP cord-001257-t21l6i3f 284 2 - - HYPH cord-001257-t21l6i3f 284 3 Fal1 Fal1 NNP cord-001257-t21l6i3f 284 4 yeast yeast NN cord-001257-t21l6i3f 284 5 was be VBD cord-001257-t21l6i3f 284 6 transformed transform VBN cord-001257-t21l6i3f 284 7 with with IN cord-001257-t21l6i3f 284 8 the the DT cord-001257-t21l6i3f 284 9 obtained obtain VBN cord-001257-t21l6i3f 284 10 Figure figure NN cord-001257-t21l6i3f 284 11 9 9 CD cord-001257-t21l6i3f 284 12 . . . cord-001257-t21l6i3f 285 1 Model model NN cord-001257-t21l6i3f 285 2 on on IN cord-001257-t21l6i3f 285 3 the the DT cord-001257-t21l6i3f 285 4 roles role NNS cord-001257-t21l6i3f 285 5 of of IN cord-001257-t21l6i3f 285 6 co co JJ cord-001257-t21l6i3f 285 7 - - JJ cord-001257-t21l6i3f 285 8 opted opt VBN cord-001257-t21l6i3f 285 9 cellular cellular JJ cord-001257-t21l6i3f 285 10 helicases helicase NNS cord-001257-t21l6i3f 285 11 in in IN cord-001257-t21l6i3f 285 12 asymmetrical asymmetrical JJ cord-001257-t21l6i3f 285 13 replication replication NN cord-001257-t21l6i3f 285 14 of of IN cord-001257-t21l6i3f 285 15 tombusviruses tombusviruse NNS cord-001257-t21l6i3f 285 16 . . . cord-001257-t21l6i3f 286 1 Cis Cis NNP cord-001257-t21l6i3f 286 2 - - HYPH cord-001257-t21l6i3f 286 3 acting act VBG cord-001257-t21l6i3f 286 4 replication replication NN cord-001257-t21l6i3f 286 5 elements element NNS cord-001257-t21l6i3f 286 6 present present JJ cord-001257-t21l6i3f 286 7 at at IN cord-001257-t21l6i3f 286 8 the the DT cord-001257-t21l6i3f 286 9 39 39 CD cord-001257-t21l6i3f 286 10 and and CC cord-001257-t21l6i3f 286 11 59 59 CD cord-001257-t21l6i3f 286 12 ends end NNS cord-001257-t21l6i3f 286 13 of of IN cord-001257-t21l6i3f 286 14 the the DT cord-001257-t21l6i3f 286 15 viral viral JJ cord-001257-t21l6i3f 286 16 ( ( -LRB- cord-001257-t21l6i3f 286 17 2)RNA 2)rna CD cord-001257-t21l6i3f 286 18 are be VBP cord-001257-t21l6i3f 286 19 recognized recognize VBN cord-001257-t21l6i3f 286 20 by by IN cord-001257-t21l6i3f 286 21 cellular cellular JJ cord-001257-t21l6i3f 286 22 helicases helicase NNS cord-001257-t21l6i3f 286 23 to to TO cord-001257-t21l6i3f 286 24 locally locally RB cord-001257-t21l6i3f 286 25 open open VB cord-001257-t21l6i3f 286 26 the the DT cord-001257-t21l6i3f 286 27 viral viral JJ cord-001257-t21l6i3f 286 28 dsRNA dsrna NN cord-001257-t21l6i3f 286 29 replication replication NN cord-001257-t21l6i3f 286 30 form form NN cord-001257-t21l6i3f 286 31 . . . cord-001257-t21l6i3f 287 1 Namely namely RB cord-001257-t21l6i3f 287 2 , , , cord-001257-t21l6i3f 287 3 the the DT cord-001257-t21l6i3f 287 4 eIF4AIII eIF4AIII NNS cord-001257-t21l6i3f 287 5 - - HYPH cord-001257-t21l6i3f 287 6 like like JJ cord-001257-t21l6i3f 287 7 helicases helicase NNS cord-001257-t21l6i3f 287 8 unwind unwind VBP cord-001257-t21l6i3f 287 9 the the DT cord-001257-t21l6i3f 287 10 dsRNA dsrna NN cord-001257-t21l6i3f 287 11 structure structure NN cord-001257-t21l6i3f 287 12 within within IN cord-001257-t21l6i3f 287 13 the the DT cord-001257-t21l6i3f 287 14 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 287 15 ) ) -RRB- cord-001257-t21l6i3f 287 16 REN REN NNP cord-001257-t21l6i3f 287 17 , , , cord-001257-t21l6i3f 287 18 while while IN cord-001257-t21l6i3f 287 19 the the DT cord-001257-t21l6i3f 287 20 DDX3-like DDX3-like NNP cord-001257-t21l6i3f 287 21 helicases helicase NNS cord-001257-t21l6i3f 287 22 open open VBP cord-001257-t21l6i3f 287 23 up up RP cord-001257-t21l6i3f 287 24 the the DT cord-001257-t21l6i3f 287 25 dsRNA dsrna NN cord-001257-t21l6i3f 287 26 structure structure NN cord-001257-t21l6i3f 287 27 within within IN cord-001257-t21l6i3f 287 28 RI(2 RI(2 NNP cord-001257-t21l6i3f 287 29 ) ) -RRB- cord-001257-t21l6i3f 287 30 ( ( -LRB- cord-001257-t21l6i3f 287 31 as as IN cord-001257-t21l6i3f 287 32 shown show VBN cord-001257-t21l6i3f 287 33 schematically schematically RB cord-001257-t21l6i3f 287 34 ) ) -RRB- cord-001257-t21l6i3f 287 35 . . . cord-001257-t21l6i3f 288 1 Long long JJ cord-001257-t21l6i3f 288 2 - - HYPH cord-001257-t21l6i3f 288 3 range range NNP cord-001257-t21l6i3f 288 4 RNA RNA NNP cord-001257-t21l6i3f 288 5 - - HYPH cord-001257-t21l6i3f 288 6 RNA RNA NNP cord-001257-t21l6i3f 288 7 interaction interaction NN cord-001257-t21l6i3f 288 8 between between IN cord-001257-t21l6i3f 288 9 the the DT cord-001257-t21l6i3f 288 10 ' ' `` cord-001257-t21l6i3f 288 11 ' ' `` cord-001257-t21l6i3f 288 12 bridge bridge NN cord-001257-t21l6i3f 288 13 ' ' '' cord-001257-t21l6i3f 288 14 ' ' '' cord-001257-t21l6i3f 288 15 and and CC cord-001257-t21l6i3f 288 16 the the DT cord-001257-t21l6i3f 288 17 cPR cPR NNP cord-001257-t21l6i3f 288 18 region region NN cord-001257-t21l6i3f 288 19 ( ( -LRB- cord-001257-t21l6i3f 288 20 promoter promoter NN cord-001257-t21l6i3f 288 21 sequence sequence NN cord-001257-t21l6i3f 288 22 at at IN cord-001257-t21l6i3f 288 23 the the DT cord-001257-t21l6i3f 288 24 39 39 CD cord-001257-t21l6i3f 288 25 end end NN cord-001257-t21l6i3f 288 26 ) ) -RRB- cord-001257-t21l6i3f 288 27 in in IN cord-001257-t21l6i3f 288 28 the the DT cord-001257-t21l6i3f 288 29 ( ( -LRB- cord-001257-t21l6i3f 288 30 2)RNA 2)rna CD cord-001257-t21l6i3f 288 31 is be VBZ cord-001257-t21l6i3f 288 32 proposed propose VBN cord-001257-t21l6i3f 288 33 to to TO cord-001257-t21l6i3f 288 34 lead lead VB cord-001257-t21l6i3f 288 35 to to IN cord-001257-t21l6i3f 288 36 circularization circularization NN cord-001257-t21l6i3f 288 37 of of IN cord-001257-t21l6i3f 288 38 the the DT cord-001257-t21l6i3f 288 39 viral viral JJ cord-001257-t21l6i3f 288 40 ( ( -LRB- cord-001257-t21l6i3f 288 41 2)RNA 2)rna CD cord-001257-t21l6i3f 288 42 , , , cord-001257-t21l6i3f 288 43 which which WDT cord-001257-t21l6i3f 288 44 is be VBZ cord-001257-t21l6i3f 288 45 still still RB cord-001257-t21l6i3f 288 46 part part NN cord-001257-t21l6i3f 288 47 of of IN cord-001257-t21l6i3f 288 48 the the DT cord-001257-t21l6i3f 288 49 dsRNA dsrna NN cord-001257-t21l6i3f 288 50 . . . cord-001257-t21l6i3f 289 1 This this DT cord-001257-t21l6i3f 289 2 RNA RNA NNP cord-001257-t21l6i3f 289 3 structure structure NN cord-001257-t21l6i3f 289 4 is be VBZ cord-001257-t21l6i3f 289 5 suggested suggest VBN cord-001257-t21l6i3f 289 6 to to TO cord-001257-t21l6i3f 289 7 facilitate facilitate VB cord-001257-t21l6i3f 289 8 the the DT cord-001257-t21l6i3f 289 9 transfer transfer NN cord-001257-t21l6i3f 289 10 of of IN cord-001257-t21l6i3f 289 11 the the DT cord-001257-t21l6i3f 289 12 viral viral JJ cord-001257-t21l6i3f 289 13 p92 p92 NN cord-001257-t21l6i3f 289 14 pol pol NN cord-001257-t21l6i3f 289 15 from from IN cord-001257-t21l6i3f 289 16 the the DT cord-001257-t21l6i3f 289 17 59 59 CD cord-001257-t21l6i3f 289 18 end end NN cord-001257-t21l6i3f 289 19 of of IN cord-001257-t21l6i3f 289 20 the the DT cord-001257-t21l6i3f 289 21 ( ( -LRB- cord-001257-t21l6i3f 289 22 2)RNA 2)rna CD cord-001257-t21l6i3f 289 23 [ [ -LRB- cord-001257-t21l6i3f 289 24 after after IN cord-001257-t21l6i3f 289 25 completion completion NN cord-001257-t21l6i3f 289 26 of of IN cord-001257-t21l6i3f 289 27 the the DT cord-001257-t21l6i3f 289 28 ( ( -LRB- cord-001257-t21l6i3f 289 29 + + CD cord-001257-t21l6i3f 289 30 ) ) -RRB- cord-001257-t21l6i3f 289 31 RNA rna NN cord-001257-t21l6i3f 289 32 strand strand NN cord-001257-t21l6i3f 289 33 synthesis synthesis NN cord-001257-t21l6i3f 289 34 in in IN cord-001257-t21l6i3f 289 35 the the DT cord-001257-t21l6i3f 289 36 previous previous JJ cord-001257-t21l6i3f 289 37 round round NN cord-001257-t21l6i3f 289 38 ] ] -RRB- cord-001257-t21l6i3f 289 39 back back NN cord-001257-t21l6i3f 289 40 to to IN cord-001257-t21l6i3f 289 41 the the DT cord-001257-t21l6i3f 289 42 39 39 CD cord-001257-t21l6i3f 289 43 cPR cPR NNS cord-001257-t21l6i3f 289 44 in in IN cord-001257-t21l6i3f 289 45 the the DT cord-001257-t21l6i3f 289 46 ( ( -LRB- cord-001257-t21l6i3f 289 47 2)RNA 2)rna CD cord-001257-t21l6i3f 289 48 for for IN cord-001257-t21l6i3f 289 49 a a DT cord-001257-t21l6i3f 289 50 new new JJ cord-001257-t21l6i3f 289 51 round round NN cord-001257-t21l6i3f 289 52 of of IN cord-001257-t21l6i3f 289 53 ( ( -LRB- cord-001257-t21l6i3f 289 54 + + CC cord-001257-t21l6i3f 289 55 ) ) -RRB- cord-001257-t21l6i3f 289 56 RNA rna NN cord-001257-t21l6i3f 289 57 strand strand NN cord-001257-t21l6i3f 289 58 synthesis synthesis NN cord-001257-t21l6i3f 289 59 . . . cord-001257-t21l6i3f 290 1 Another another DT cord-001257-t21l6i3f 290 2 co co NN cord-001257-t21l6i3f 290 3 - - JJ cord-001257-t21l6i3f 290 4 opted opt VBN cord-001257-t21l6i3f 290 5 cellular cellular JJ cord-001257-t21l6i3f 290 6 protein protein NN cord-001257-t21l6i3f 290 7 , , , cord-001257-t21l6i3f 290 8 GAPDH GAPDH NNP cord-001257-t21l6i3f 290 9 ( ( -LRB- cord-001257-t21l6i3f 290 10 called call VBN cord-001257-t21l6i3f 290 11 Tdh2/3 Tdh2/3 NNP cord-001257-t21l6i3f 290 12 in in IN cord-001257-t21l6i3f 290 13 yeast yeast NN cord-001257-t21l6i3f 290 14 ) ) -RRB- cord-001257-t21l6i3f 290 15 is be VBZ cord-001257-t21l6i3f 290 16 likely likely RB cord-001257-t21l6i3f 290 17 involved involve VBN cord-001257-t21l6i3f 290 18 in in IN cord-001257-t21l6i3f 290 19 this this DT cord-001257-t21l6i3f 290 20 process process NN cord-001257-t21l6i3f 290 21 by by IN cord-001257-t21l6i3f 290 22 facilitating facilitate VBG cord-001257-t21l6i3f 290 23 the the DT cord-001257-t21l6i3f 290 24 binding binding NN cord-001257-t21l6i3f 290 25 of of IN cord-001257-t21l6i3f 290 26 p92 p92 CD cord-001257-t21l6i3f 290 27 pol pol NN cord-001257-t21l6i3f 290 28 to to IN cord-001257-t21l6i3f 290 29 the the DT cord-001257-t21l6i3f 290 30 39end 39end NN cord-001257-t21l6i3f 290 31 of of IN cord-001257-t21l6i3f 290 32 the the DT cord-001257-t21l6i3f 290 33 ( ( -LRB- cord-001257-t21l6i3f 290 34 2)RNA 2)rna CD cord-001257-t21l6i3f 290 35 . . . cord-001257-t21l6i3f 291 1 Altogether altogether RB cord-001257-t21l6i3f 291 2 , , , cord-001257-t21l6i3f 291 3 the the DT cord-001257-t21l6i3f 291 4 re re NN cord-001257-t21l6i3f 291 5 - - NN cord-001257-t21l6i3f 291 6 use use NN cord-001257-t21l6i3f 291 7 of of IN cord-001257-t21l6i3f 291 8 the the DT cord-001257-t21l6i3f 291 9 viral viral JJ cord-001257-t21l6i3f 291 10 p92 p92 NN cord-001257-t21l6i3f 291 11 pol pol NN cord-001257-t21l6i3f 291 12 multiple multiple JJ cord-001257-t21l6i3f 291 13 times time NNS cord-001257-t21l6i3f 291 14 on on IN cord-001257-t21l6i3f 291 15 the the DT cord-001257-t21l6i3f 291 16 same same JJ cord-001257-t21l6i3f 291 17 ( ( -LRB- cord-001257-t21l6i3f 291 18 2 2 LS cord-001257-t21l6i3f 291 19 ) ) -RRB- cord-001257-t21l6i3f 291 20 RNA rna NN cord-001257-t21l6i3f 291 21 template template NN cord-001257-t21l6i3f 291 22 could could MD cord-001257-t21l6i3f 291 23 result result VB cord-001257-t21l6i3f 291 24 in in IN cord-001257-t21l6i3f 291 25 production production NN cord-001257-t21l6i3f 291 26 of of IN cord-001257-t21l6i3f 291 27 multiple multiple JJ cord-001257-t21l6i3f 291 28 ( ( -LRB- cord-001257-t21l6i3f 291 29 + + CC cord-001257-t21l6i3f 291 30 ) ) -RRB- cord-001257-t21l6i3f 291 31 RNA rna NN cord-001257-t21l6i3f 291 32 progeny progeny NN cord-001257-t21l6i3f 291 33 , , , cord-001257-t21l6i3f 291 34 resulting result VBG cord-001257-t21l6i3f 291 35 in in IN cord-001257-t21l6i3f 291 36 the the DT cord-001257-t21l6i3f 291 37 characteristic characteristic JJ cord-001257-t21l6i3f 291 38 asymmetric asymmetric JJ cord-001257-t21l6i3f 291 39 viral viral JJ cord-001257-t21l6i3f 291 40 RNA RNA NNP cord-001257-t21l6i3f 291 41 replication replication NN cord-001257-t21l6i3f 291 42 . . . cord-001257-t21l6i3f 292 1 Note note VB cord-001257-t21l6i3f 292 2 that that IN cord-001257-t21l6i3f 292 3 it -PRON- PRP cord-001257-t21l6i3f 292 4 is be VBZ cord-001257-t21l6i3f 292 5 also also RB cord-001257-t21l6i3f 292 6 possible possible JJ cord-001257-t21l6i3f 292 7 that that IN cord-001257-t21l6i3f 292 8 p92 p92 NN cord-001257-t21l6i3f 292 9 pol pol NN cord-001257-t21l6i3f 292 10 is be VBZ cord-001257-t21l6i3f 292 11 standing stand VBG cord-001257-t21l6i3f 292 12 still still RB cord-001257-t21l6i3f 292 13 , , , cord-001257-t21l6i3f 292 14 while while IN cord-001257-t21l6i3f 292 15 the the DT cord-001257-t21l6i3f 292 16 RNA RNA NNP cord-001257-t21l6i3f 292 17 template template NN cord-001257-t21l6i3f 292 18 is be VBZ cord-001257-t21l6i3f 292 19 moved move VBN cord-001257-t21l6i3f 292 20 during during IN cord-001257-t21l6i3f 292 21 RNA RNA NNP cord-001257-t21l6i3f 292 22 synthesis synthesis NN cord-001257-t21l6i3f 292 23 within within IN cord-001257-t21l6i3f 292 24 the the DT cord-001257-t21l6i3f 292 25 membrane membrane NN cord-001257-t21l6i3f 292 26 - - HYPH cord-001257-t21l6i3f 292 27 bound bind VBN cord-001257-t21l6i3f 292 28 VRCs vrcs NN cord-001257-t21l6i3f 292 29 . . . cord-001257-t21l6i3f 293 1 doi:10.1371 doi:10.1371 NNP cord-001257-t21l6i3f 293 2 / / SYM cord-001257-t21l6i3f 293 3 journal.ppat.1004051.g009 journal.ppat.1004051.g009 NNP cord-001257-t21l6i3f 293 4 PCR PCR NNP cord-001257-t21l6i3f 293 5 product product NN cord-001257-t21l6i3f 293 6 and and CC cord-001257-t21l6i3f 293 7 the the DT cord-001257-t21l6i3f 293 8 suitable suitable JJ cord-001257-t21l6i3f 293 9 yeast yeast NN cord-001257-t21l6i3f 293 10 strain strain NN cord-001257-t21l6i3f 293 11 was be VBD cord-001257-t21l6i3f 293 12 selected select VBN cord-001257-t21l6i3f 293 13 on on IN cord-001257-t21l6i3f 293 14 G418 G418 NNS cord-001257-t21l6i3f 293 15 containing contain VBG cord-001257-t21l6i3f 293 16 plates plate NNS cord-001257-t21l6i3f 293 17 . . . cord-001257-t21l6i3f 294 1 Then then RB cord-001257-t21l6i3f 294 2 , , , cord-001257-t21l6i3f 294 3 yeast yeast NN cord-001257-t21l6i3f 294 4 strains strain NNS cord-001257-t21l6i3f 294 5 were be VBD cord-001257-t21l6i3f 294 6 grown grow VBN cord-001257-t21l6i3f 294 7 in in IN cord-001257-t21l6i3f 294 8 liquid liquid JJ cord-001257-t21l6i3f 294 9 media medium NNS cord-001257-t21l6i3f 294 10 and and CC cord-001257-t21l6i3f 294 11 genomic genomic JJ cord-001257-t21l6i3f 294 12 DNA dna NN cord-001257-t21l6i3f 294 13 was be VBD cord-001257-t21l6i3f 294 14 isolated isolate VBN cord-001257-t21l6i3f 294 15 . . . cord-001257-t21l6i3f 295 1 The the DT cord-001257-t21l6i3f 295 2 correct correct JJ cord-001257-t21l6i3f 295 3 deletion deletion NN cord-001257-t21l6i3f 295 4 site site NN cord-001257-t21l6i3f 295 5 was be VBD cord-001257-t21l6i3f 295 6 checked check VBN cord-001257-t21l6i3f 295 7 by by IN cord-001257-t21l6i3f 295 8 PCR PCR NNP cord-001257-t21l6i3f 295 9 with with IN cord-001257-t21l6i3f 295 10 primers primer NNS cord-001257-t21l6i3f 295 11 # # $ cord-001257-t21l6i3f 295 12 2215 2215 CD cord-001257-t21l6i3f 295 13 and and CC cord-001257-t21l6i3f 295 14 # # $ cord-001257-t21l6i3f 295 15 5019 5019 CD cord-001257-t21l6i3f 295 16 using use VBG cord-001257-t21l6i3f 295 17 genomic genomic JJ cord-001257-t21l6i3f 295 18 DNA dna NN cord-001257-t21l6i3f 295 19 as as IN cord-001257-t21l6i3f 295 20 a a DT cord-001257-t21l6i3f 295 21 template template NN cord-001257-t21l6i3f 295 22 . . . cord-001257-t21l6i3f 296 1 PCR PCR NNP cord-001257-t21l6i3f 296 2 products product NNS cord-001257-t21l6i3f 296 3 of of IN cord-001257-t21l6i3f 296 4 yeast yeast NN cord-001257-t21l6i3f 296 5 helicase helicase NN cord-001257-t21l6i3f 296 6 genes gene NNS cord-001257-t21l6i3f 296 7 were be VBD cord-001257-t21l6i3f 296 8 obtained obtain VBN cord-001257-t21l6i3f 296 9 as as IN cord-001257-t21l6i3f 296 10 follows follow VBZ cord-001257-t21l6i3f 296 11 : : : cord-001257-t21l6i3f 296 12 yeast yeast NN cord-001257-t21l6i3f 296 13 genomic genomic JJ cord-001257-t21l6i3f 296 14 DNA dna NN cord-001257-t21l6i3f 296 15 was be VBD cord-001257-t21l6i3f 296 16 used use VBN cord-001257-t21l6i3f 296 17 as as IN cord-001257-t21l6i3f 296 18 a a DT cord-001257-t21l6i3f 296 19 template template NN cord-001257-t21l6i3f 296 20 for for IN cord-001257-t21l6i3f 296 21 amplification amplification NN cord-001257-t21l6i3f 296 22 by by IN cord-001257-t21l6i3f 296 23 PCR PCR NNP cord-001257-t21l6i3f 296 24 with with IN cord-001257-t21l6i3f 296 25 primers primer NNS cord-001257-t21l6i3f 296 26 # # $ cord-001257-t21l6i3f 296 27 4612 4612 CD cord-001257-t21l6i3f 296 28 and and CC cord-001257-t21l6i3f 296 29 # # $ cord-001257-t21l6i3f 296 30 4613 4613 CD cord-001257-t21l6i3f 296 31 for for IN cord-001257-t21l6i3f 296 32 DBP3 DBP3 NNP cord-001257-t21l6i3f 296 33 ; ; : cord-001257-t21l6i3f 296 34 # # $ cord-001257-t21l6i3f 296 35 4569 4569 CD cord-001257-t21l6i3f 296 36 and and CC cord-001257-t21l6i3f 296 37 # # $ cord-001257-t21l6i3f 296 38 4570 4570 CD cord-001257-t21l6i3f 296 39 for for IN cord-001257-t21l6i3f 296 40 DBP5 DBP5 NNP cord-001257-t21l6i3f 296 41 ; ; : cord-001257-t21l6i3f 296 42 # # $ cord-001257-t21l6i3f 296 43 4611 4611 CD cord-001257-t21l6i3f 296 44 and and CC cord-001257-t21l6i3f 296 45 # # $ cord-001257-t21l6i3f 296 46 4756 4756 CD cord-001257-t21l6i3f 296 47 for for IN cord-001257-t21l6i3f 296 48 DBP7 DBP7 NNP cord-001257-t21l6i3f 296 49 ; ; : cord-001257-t21l6i3f 296 50 # # $ cord-001257-t21l6i3f 296 51 2351 2351 CD cord-001257-t21l6i3f 296 52 and and CC cord-001257-t21l6i3f 296 53 4825 4825 CD cord-001257-t21l6i3f 296 54 for for IN cord-001257-t21l6i3f 296 55 TIF1 TIF1 NNP cord-001257-t21l6i3f 296 56 ; ; : cord-001257-t21l6i3f 296 57 and and CC cord-001257-t21l6i3f 296 58 # # $ cord-001257-t21l6i3f 296 59 4893 4893 CD cord-001257-t21l6i3f 296 60 and and CC cord-001257-t21l6i3f 296 61 # # $ cord-001257-t21l6i3f 296 62 4894 4894 CD cord-001257-t21l6i3f 296 63 for for IN cord-001257-t21l6i3f 296 64 FAL1 FAL1 NNP cord-001257-t21l6i3f 296 65 . . . cord-001257-t21l6i3f 297 1 The the DT cord-001257-t21l6i3f 297 2 generated generate VBN cord-001257-t21l6i3f 297 3 PCR PCR NNP cord-001257-t21l6i3f 297 4 products product NNS cord-001257-t21l6i3f 297 5 were be VBD cord-001257-t21l6i3f 297 6 digested digest VBN cord-001257-t21l6i3f 297 7 with with IN cord-001257-t21l6i3f 297 8 BamHI BamHI NNP cord-001257-t21l6i3f 297 9 and and CC cord-001257-t21l6i3f 297 10 XbaI XbaI NNS cord-001257-t21l6i3f 297 11 in in IN cord-001257-t21l6i3f 297 12 the the DT cord-001257-t21l6i3f 297 13 case case NN cord-001257-t21l6i3f 297 14 of of IN cord-001257-t21l6i3f 297 15 DBP3 DBP3 NNP cord-001257-t21l6i3f 297 16 and and CC cord-001257-t21l6i3f 297 17 DBP5 DBP5 NNP cord-001257-t21l6i3f 297 18 and and CC cord-001257-t21l6i3f 297 19 with with IN cord-001257-t21l6i3f 297 20 BglII BglII NNP cord-001257-t21l6i3f 297 21 and and CC cord-001257-t21l6i3f 297 22 XbaI XbaI NNP cord-001257-t21l6i3f 297 23 in in IN cord-001257-t21l6i3f 297 24 the the DT cord-001257-t21l6i3f 297 25 case case NN cord-001257-t21l6i3f 297 26 of of IN cord-001257-t21l6i3f 297 27 DBP7 DBP7 NNP cord-001257-t21l6i3f 297 28 . . . cord-001257-t21l6i3f 298 1 Plasmids plasmid NNS cord-001257-t21l6i3f 298 2 pYC- pyc- XX cord-001257-t21l6i3f 299 1 His -PRON- PRP$ cord-001257-t21l6i3f 299 2 ( ( -LRB- cord-001257-t21l6i3f 299 3 provided provide VBN cord-001257-t21l6i3f 299 4 by by IN cord-001257-t21l6i3f 299 5 Dr. Dr. NNP cord-001257-t21l6i3f 299 6 Daniel Daniel NNP cord-001257-t21l6i3f 299 7 Barajas Barajas NNP cord-001257-t21l6i3f 299 8 ) ) -RRB- cord-001257-t21l6i3f 299 9 and and CC cord-001257-t21l6i3f 299 10 pMalc-2x pMalc-2x NNP cord-001257-t21l6i3f 299 11 ( ( -LRB- cord-001257-t21l6i3f 299 12 New New NNP cord-001257-t21l6i3f 299 13 England England NNP cord-001257-t21l6i3f 299 14 Biolabs Biolabs NNP cord-001257-t21l6i3f 299 15 ) ) -RRB- cord-001257-t21l6i3f 299 16 were be VBD cord-001257-t21l6i3f 299 17 digested digest VBN cord-001257-t21l6i3f 299 18 with with IN cord-001257-t21l6i3f 299 19 BamHI BamHI NNP cord-001257-t21l6i3f 299 20 and and CC cord-001257-t21l6i3f 299 21 XbaI XbaI NNP cord-001257-t21l6i3f 299 22 and and CC cord-001257-t21l6i3f 299 23 pPr pPr NNPS cord-001257-t21l6i3f 299 24 - - HYPH cord-001257-t21l6i3f 299 25 N N NNP cord-001257-t21l6i3f 299 26 ( ( -LRB- cord-001257-t21l6i3f 299 27 Dualsystems Dualsystems NNP cord-001257-t21l6i3f 299 28 ) ) -RRB- cord-001257-t21l6i3f 299 29 was be VBD cord-001257-t21l6i3f 299 30 digested digest VBN cord-001257-t21l6i3f 299 31 with with IN cord-001257-t21l6i3f 299 32 BamHI BamHI NNP cord-001257-t21l6i3f 299 33 and and CC cord-001257-t21l6i3f 299 34 NheI NheI NNP cord-001257-t21l6i3f 299 35 and and CC cord-001257-t21l6i3f 299 36 ligated ligate VBD cord-001257-t21l6i3f 299 37 with with IN cord-001257-t21l6i3f 299 38 the the DT cord-001257-t21l6i3f 299 39 similarly similarly RB cord-001257-t21l6i3f 299 40 treated treat VBN cord-001257-t21l6i3f 299 41 PCR PCR NNP cord-001257-t21l6i3f 299 42 products product NNS cord-001257-t21l6i3f 299 43 of of IN cord-001257-t21l6i3f 299 44 DBP3 DBP3 NNP cord-001257-t21l6i3f 299 45 , , , cord-001257-t21l6i3f 299 46 DBP5 DBP5 NNP cord-001257-t21l6i3f 299 47 and and CC cord-001257-t21l6i3f 299 48 DBP7 DBP7 NNP cord-001257-t21l6i3f 299 49 . . . cord-001257-t21l6i3f 300 1 The the DT cord-001257-t21l6i3f 300 2 PCR PCR NNP cord-001257-t21l6i3f 300 3 products product NNS cord-001257-t21l6i3f 300 4 of of IN cord-001257-t21l6i3f 300 5 plant plant NN cord-001257-t21l6i3f 300 6 helicases helicase NNS cord-001257-t21l6i3f 300 7 were be VBD cord-001257-t21l6i3f 300 8 obtained obtain VBN cord-001257-t21l6i3f 300 9 as as IN cord-001257-t21l6i3f 300 10 follows follow VBZ cord-001257-t21l6i3f 300 11 : : : cord-001257-t21l6i3f 301 1 Total Total NNP cord-001257-t21l6i3f 301 2 RNA RNA NNP cord-001257-t21l6i3f 301 3 was be VBD cord-001257-t21l6i3f 301 4 isolated isolate VBN cord-001257-t21l6i3f 301 5 from from IN cord-001257-t21l6i3f 301 6 A. a. NN cord-001257-t21l6i3f 301 7 thaliana thaliana NN cord-001257-t21l6i3f 301 8 and and CC cord-001257-t21l6i3f 301 9 used use VBN cord-001257-t21l6i3f 301 10 for for IN cord-001257-t21l6i3f 301 11 RT RT NNP cord-001257-t21l6i3f 301 12 - - HYPH cord-001257-t21l6i3f 301 13 PCR PCR NNP cord-001257-t21l6i3f 301 14 with with IN cord-001257-t21l6i3f 301 15 primers primer NNS cord-001257-t21l6i3f 301 16 # # $ cord-001257-t21l6i3f 301 17 4816 4816 CD cord-001257-t21l6i3f 301 18 and and CC cord-001257-t21l6i3f 301 19 # # $ cord-001257-t21l6i3f 301 20 4817 4817 CD cord-001257-t21l6i3f 301 21 for for IN cord-001257-t21l6i3f 301 22 AtRH2 atrh2 NN cord-001257-t21l6i3f 301 23 ; ; : cord-001257-t21l6i3f 301 24 # # $ cord-001257-t21l6i3f 301 25 4813 4813 CD cord-001257-t21l6i3f 301 26 and and CC cord-001257-t21l6i3f 301 27 # # $ cord-001257-t21l6i3f 301 28 4871 4871 CD cord-001257-t21l6i3f 301 29 for for IN cord-001257-t21l6i3f 301 30 AtRH4 atrh4 NN cord-001257-t21l6i3f 301 31 ; ; : cord-001257-t21l6i3f 301 32 # # $ cord-001257-t21l6i3f 301 33 4819 4819 CD cord-001257-t21l6i3f 301 34 and and CC cord-001257-t21l6i3f 301 35 # # $ cord-001257-t21l6i3f 301 36 4820 4820 CD cord-001257-t21l6i3f 301 37 for for IN cord-001257-t21l6i3f 301 38 AtRH5 atrh5 NN cord-001257-t21l6i3f 301 39 ; ; , cord-001257-t21l6i3f 301 40 and and CC cord-001257-t21l6i3f 301 41 # # $ cord-001257-t21l6i3f 301 42 4822 4822 CD cord-001257-t21l6i3f 301 43 and and CC cord-001257-t21l6i3f 301 44 # # $ cord-001257-t21l6i3f 301 45 4823 4823 CD cord-001257-t21l6i3f 301 46 for for IN cord-001257-t21l6i3f 301 47 AtRH7 AtRH7 NNP cord-001257-t21l6i3f 301 48 . . . cord-001257-t21l6i3f 302 1 The the DT cord-001257-t21l6i3f 302 2 obtained obtain VBN cord-001257-t21l6i3f 302 3 PCR PCR NNP cord-001257-t21l6i3f 302 4 products product NNS cord-001257-t21l6i3f 302 5 were be VBD cord-001257-t21l6i3f 302 6 digested digest VBN cord-001257-t21l6i3f 302 7 with with IN cord-001257-t21l6i3f 302 8 BamHI BamHI NNP cord-001257-t21l6i3f 302 9 and and CC cord-001257-t21l6i3f 302 10 SalI SalI NNP cord-001257-t21l6i3f 302 11 in in IN cord-001257-t21l6i3f 302 12 the the DT cord-001257-t21l6i3f 302 13 case case NN cord-001257-t21l6i3f 302 14 of of IN cord-001257-t21l6i3f 302 15 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 302 16 , , , cord-001257-t21l6i3f 302 17 AtRH4 AtRH4 NNP cord-001257-t21l6i3f 302 18 , , , cord-001257-t21l6i3f 302 19 AtRH7 AtRH7 NNP cord-001257-t21l6i3f 302 20 , , , cord-001257-t21l6i3f 302 21 FAL1 FAL1 NNP cord-001257-t21l6i3f 302 22 and and CC cord-001257-t21l6i3f 302 23 TIF1 TIF1 NNP cord-001257-t21l6i3f 302 24 and and CC cord-001257-t21l6i3f 302 25 BglII BglII NNP cord-001257-t21l6i3f 302 26 and and CC cord-001257-t21l6i3f 302 27 SalI SalI NNP cord-001257-t21l6i3f 302 28 in in IN cord-001257-t21l6i3f 302 29 the the DT cord-001257-t21l6i3f 302 30 case case NN cord-001257-t21l6i3f 302 31 of of IN cord-001257-t21l6i3f 302 32 AtRH5 atrh5 NN cord-001257-t21l6i3f 302 33 . . . cord-001257-t21l6i3f 303 1 Plasmid Plasmid NNP cord-001257-t21l6i3f 303 2 pYC- pyc- CD cord-001257-t21l6i3f 304 1 His -PRON- PRP$ cord-001257-t21l6i3f 304 2 was be VBD cord-001257-t21l6i3f 304 3 digested digest VBN cord-001257-t21l6i3f 304 4 with with IN cord-001257-t21l6i3f 304 5 BamHI BamHI NNP cord-001257-t21l6i3f 304 6 and and CC cord-001257-t21l6i3f 304 7 XhoI XhoI NNP cord-001257-t21l6i3f 304 8 and and CC cord-001257-t21l6i3f 304 9 plasmids plasmid NNS cord-001257-t21l6i3f 304 10 pMalc-2x pMalc-2x NNP cord-001257-t21l6i3f 304 11 , , , cord-001257-t21l6i3f 304 12 pPr pPr NNPS cord-001257-t21l6i3f 304 13 - - HYPH cord-001257-t21l6i3f 304 14 N N NNP cord-001257-t21l6i3f 304 15 , , , cord-001257-t21l6i3f 304 16 pET-30c(+ pET-30c(+ NNP cord-001257-t21l6i3f 304 17 ) ) -RRB- cord-001257-t21l6i3f 304 18 ( ( -LRB- cord-001257-t21l6i3f 304 19 for for IN cord-001257-t21l6i3f 304 20 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 304 21 and and CC cord-001257-t21l6i3f 304 22 AtRH5 AtRH5 NNP cord-001257-t21l6i3f 304 23 ) ) -RRB- cord-001257-t21l6i3f 304 24 , , , cord-001257-t21l6i3f 304 25 and and CC cord-001257-t21l6i3f 304 26 pGD-35S pgd-35s NN cord-001257-t21l6i3f 304 27 ( ( -LRB- cord-001257-t21l6i3f 304 28 for for IN cord-001257-t21l6i3f 304 29 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 304 30 , , , cord-001257-t21l6i3f 304 31 AtRH4 AtRH4 NNP cord-001257-t21l6i3f 304 32 , , , cord-001257-t21l6i3f 304 33 AtRH5 AtRH5 NNP cord-001257-t21l6i3f 304 34 and and CC cord-001257-t21l6i3f 304 35 AtRH7 AtRH7 NNP cord-001257-t21l6i3f 304 36 ) ) -RRB- cord-001257-t21l6i3f 304 37 were be VBD cord-001257-t21l6i3f 304 38 digested digest VBN cord-001257-t21l6i3f 304 39 with with IN cord-001257-t21l6i3f 304 40 BamHI BamHI NNP cord-001257-t21l6i3f 304 41 and and CC cord-001257-t21l6i3f 304 42 SalI SalI NNP cord-001257-t21l6i3f 304 43 and and CC cord-001257-t21l6i3f 304 44 ligated ligate VBD cord-001257-t21l6i3f 304 45 to to IN cord-001257-t21l6i3f 304 46 similarly similarly RB cord-001257-t21l6i3f 304 47 treated treat VBN cord-001257-t21l6i3f 304 48 PCR PCR NNP cord-001257-t21l6i3f 304 49 products product NNS cord-001257-t21l6i3f 304 50 of of IN cord-001257-t21l6i3f 304 51 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 304 52 , , , cord-001257-t21l6i3f 304 53 AtRH4 AtRH4 NNP cord-001257-t21l6i3f 304 54 , , , cord-001257-t21l6i3f 304 55 AtRH5 AtRH5 NNP cord-001257-t21l6i3f 304 56 and and CC cord-001257-t21l6i3f 304 57 AtRH7 AtRH7 NNP cord-001257-t21l6i3f 304 58 , , , cord-001257-t21l6i3f 304 59 FAL1 FAL1 NNP cord-001257-t21l6i3f 304 60 and and CC cord-001257-t21l6i3f 304 61 TIF1 TIF1 NNP cord-001257-t21l6i3f 304 62 . . . cord-001257-t21l6i3f 305 1 Overexpression overexpression NN cord-001257-t21l6i3f 305 2 plasmid plasmid NN cord-001257-t21l6i3f 305 3 pGD pGD NNP cord-001257-t21l6i3f 305 4 - - : cord-001257-t21l6i3f 305 5 RH20 RH20 NNP cord-001257-t21l6i3f 305 6 was be VBD cord-001257-t21l6i3f 305 7 obtained obtain VBN cord-001257-t21l6i3f 305 8 as as IN cord-001257-t21l6i3f 305 9 follows follow VBZ cord-001257-t21l6i3f 305 10 : : : cord-001257-t21l6i3f 306 1 AtRH20 AtRH20 NNP cord-001257-t21l6i3f 306 2 sequence sequence NN cord-001257-t21l6i3f 306 3 was be VBD cord-001257-t21l6i3f 306 4 amplified amplify VBN cord-001257-t21l6i3f 306 5 using use VBG cord-001257-t21l6i3f 306 6 primers primer NNS cord-001257-t21l6i3f 306 7 # # $ cord-001257-t21l6i3f 306 8 4318 4318 CD cord-001257-t21l6i3f 306 9 and and CC cord-001257-t21l6i3f 306 10 # # $ cord-001257-t21l6i3f 306 11 4473 4473 CD cord-001257-t21l6i3f 306 12 and and CC cord-001257-t21l6i3f 307 1 pMAL pmal JJ cord-001257-t21l6i3f 307 2 - - HYPH cord-001257-t21l6i3f 307 3 RH20 RH20 NNP cord-001257-t21l6i3f 307 4 [ [ -LRB- cord-001257-t21l6i3f 307 5 49 49 CD cord-001257-t21l6i3f 307 6 ] ] -RRB- cord-001257-t21l6i3f 307 7 as as IN cord-001257-t21l6i3f 307 8 a a DT cord-001257-t21l6i3f 307 9 template template NN cord-001257-t21l6i3f 307 10 . . . cord-001257-t21l6i3f 308 1 The the DT cord-001257-t21l6i3f 308 2 obtained obtain VBN cord-001257-t21l6i3f 308 3 PCR pcr NN cord-001257-t21l6i3f 308 4 product product NN cord-001257-t21l6i3f 308 5 was be VBD cord-001257-t21l6i3f 308 6 digested digest VBN cord-001257-t21l6i3f 308 7 with with IN cord-001257-t21l6i3f 308 8 BamHI BamHI NNP cord-001257-t21l6i3f 308 9 and and CC cord-001257-t21l6i3f 308 10 SpeI SpeI NNP cord-001257-t21l6i3f 308 11 and and CC cord-001257-t21l6i3f 308 12 inserted insert VBD cord-001257-t21l6i3f 308 13 into into IN cord-001257-t21l6i3f 308 14 pGD-35S pgd-35s JJ cord-001257-t21l6i3f 308 15 plasmid plasmid NN cord-001257-t21l6i3f 308 16 , , , cord-001257-t21l6i3f 308 17 which which WDT cord-001257-t21l6i3f 308 18 was be VBD cord-001257-t21l6i3f 308 19 digested digest VBN cord-001257-t21l6i3f 308 20 with with IN cord-001257-t21l6i3f 308 21 BamHI bamhi NN cord-001257-t21l6i3f 308 22 and and CC cord-001257-t21l6i3f 308 23 XbaI. xbai. NN cord-001257-t21l6i3f 309 1 The the DT cord-001257-t21l6i3f 309 2 plasmids plasmid NNS cord-001257-t21l6i3f 310 1 pGBK pgbk JJ cord-001257-t21l6i3f 310 2 - - HYPH cord-001257-t21l6i3f 310 3 HIS HIS NNP cord-001257-t21l6i3f 310 4 - - HYPH cord-001257-t21l6i3f 310 5 Cup Cup NNP cord-001257-t21l6i3f 310 6 - - HYPH cord-001257-t21l6i3f 310 7 Flag33 Flag33 NNP cord-001257-t21l6i3f 310 8 / / SYM cord-001257-t21l6i3f 310 9 Gal Gal NNP cord-001257-t21l6i3f 310 10 - - HYPH cord-001257-t21l6i3f 310 11 DI-72 DI-72 NNP cord-001257-t21l6i3f 310 12 expressing express VBG cord-001257-t21l6i3f 310 13 Flag Flag NNP cord-001257-t21l6i3f 310 14 - - HYPH cord-001257-t21l6i3f 310 15 tagged tag VBN cord-001257-t21l6i3f 310 16 p33 p33 NN cord-001257-t21l6i3f 310 17 of of IN cord-001257-t21l6i3f 310 18 cucumber cucumber NN cord-001257-t21l6i3f 310 19 necrosis necrosis NN cord-001257-t21l6i3f 310 20 virus virus NN cord-001257-t21l6i3f 310 21 ( ( -LRB- cord-001257-t21l6i3f 310 22 CNV CNV NNP cord-001257-t21l6i3f 310 23 ) ) -RRB- cord-001257-t21l6i3f 310 24 and and CC cord-001257-t21l6i3f 310 25 the the DT cord-001257-t21l6i3f 310 26 TBSV TBSV NNP cord-001257-t21l6i3f 310 27 DI-72 DI-72 NNP cord-001257-t21l6i3f 310 28 repRNA repRNA VBZ cord-001257-t21l6i3f 310 29 [ [ -LRB- cord-001257-t21l6i3f 310 30 93 93 CD cord-001257-t21l6i3f 310 31 ] ] -RRB- cord-001257-t21l6i3f 310 32 , , , cord-001257-t21l6i3f 310 33 pGAD pGAD NNP cord-001257-t21l6i3f 310 34 - - HYPH cord-001257-t21l6i3f 310 35 Cup Cup NNP cord-001257-t21l6i3f 310 36 - - HYPH cord-001257-t21l6i3f 310 37 Flag92 Flag92 NNP cord-001257-t21l6i3f 310 38 [ [ -LRB- cord-001257-t21l6i3f 310 39 94 94 CD cord-001257-t21l6i3f 310 40 ] ] -RRB- cord-001257-t21l6i3f 310 41 , , , cord-001257-t21l6i3f 310 42 pGD pGD NNP cord-001257-t21l6i3f 310 43 - - HYPH cord-001257-t21l6i3f 310 44 CNV CNV NNP cord-001257-t21l6i3f 310 45 and and CC cord-001257-t21l6i3f 310 46 pGD pGD NNP cord-001257-t21l6i3f 310 47 - - HYPH cord-001257-t21l6i3f 310 48 p19 p19 NN cord-001257-t21l6i3f 310 49 [ [ -LRB- cord-001257-t21l6i3f 310 50 95 95 CD cord-001257-t21l6i3f 310 51 ] ] -RRB- cord-001257-t21l6i3f 310 52 were be VBD cord-001257-t21l6i3f 310 53 described describe VBN cord-001257-t21l6i3f 310 54 earlier early RBR cord-001257-t21l6i3f 310 55 . . . cord-001257-t21l6i3f 311 1 Cultures culture NNS cord-001257-t21l6i3f 311 2 of of IN cord-001257-t21l6i3f 311 3 Agrobacterium agrobacterium NN cord-001257-t21l6i3f 311 4 tumefaciens tumefaciens NNP cord-001257-t21l6i3f 312 1 C58C1 C58C1 NNP cord-001257-t21l6i3f 312 2 strain strain VB cord-001257-t21l6i3f 312 3 carrying carry VBG cord-001257-t21l6i3f 312 4 pGD pgd NN cord-001257-t21l6i3f 312 5 - - HYPH cord-001257-t21l6i3f 312 6 RH2 RH2 NNP cord-001257-t21l6i3f 312 7 , , , cord-001257-t21l6i3f 312 8 pGD pgd NN cord-001257-t21l6i3f 312 9 - - HYPH cord-001257-t21l6i3f 312 10 RH5 rh5 NN cord-001257-t21l6i3f 312 11 , , , cord-001257-t21l6i3f 312 12 pGD pgd NN cord-001257-t21l6i3f 312 13 - - HYPH cord-001257-t21l6i3f 312 14 RH20 RH20 NNP cord-001257-t21l6i3f 312 15 ( ( -LRB- cord-001257-t21l6i3f 312 16 individually individually RB cord-001257-t21l6i3f 312 17 ) ) -RRB- cord-001257-t21l6i3f 312 18 with with IN cord-001257-t21l6i3f 312 19 pGD pGD NNP cord-001257-t21l6i3f 312 20 - - HYPH cord-001257-t21l6i3f 312 21 CNV CNV NNP cord-001257-t21l6i3f 312 22 and and CC cord-001257-t21l6i3f 312 23 pGD pGD NNP cord-001257-t21l6i3f 312 24 - - HYPH cord-001257-t21l6i3f 312 25 p19 p19 NN cord-001257-t21l6i3f 312 26 were be VBD cord-001257-t21l6i3f 312 27 prepared prepare VBN cord-001257-t21l6i3f 312 28 and and CC cord-001257-t21l6i3f 312 29 infiltrated infiltrate VBN cord-001257-t21l6i3f 312 30 into into IN cord-001257-t21l6i3f 312 31 leaves leave NNS cord-001257-t21l6i3f 312 32 of of IN cord-001257-t21l6i3f 312 33 N. N. NNP cord-001257-t21l6i3f 312 34 benthamiana benthamiana NNP cord-001257-t21l6i3f 312 35 as as IN cord-001257-t21l6i3f 312 36 described describe VBN cord-001257-t21l6i3f 312 37 earlier early RBR cord-001257-t21l6i3f 312 38 [ [ -LRB- cord-001257-t21l6i3f 312 39 95 95 CD cord-001257-t21l6i3f 312 40 ] ] -RRB- cord-001257-t21l6i3f 312 41 . . . cord-001257-t21l6i3f 313 1 Agrobacterium agrobacterium NN cord-001257-t21l6i3f 313 2 culture culture NN cord-001257-t21l6i3f 313 3 carrying carry VBG cord-001257-t21l6i3f 313 4 empty empty JJ cord-001257-t21l6i3f 313 5 pGD-35S pgd-35s CD cord-001257-t21l6i3f 313 6 plasmid plasmid NN cord-001257-t21l6i3f 313 7 was be VBD cord-001257-t21l6i3f 313 8 used use VBN cord-001257-t21l6i3f 313 9 as as IN cord-001257-t21l6i3f 313 10 a a DT cord-001257-t21l6i3f 313 11 negative negative JJ cord-001257-t21l6i3f 313 12 control control NN cord-001257-t21l6i3f 313 13 . . . cord-001257-t21l6i3f 314 1 During during IN cord-001257-t21l6i3f 314 2 multiple multiple JJ cord-001257-t21l6i3f 314 3 overexpression overexpression NN cord-001257-t21l6i3f 314 4 , , , cord-001257-t21l6i3f 314 5 we -PRON- PRP cord-001257-t21l6i3f 314 6 used use VBD cord-001257-t21l6i3f 314 7 the the DT cord-001257-t21l6i3f 314 8 Agrobacterium agrobacterium NN cord-001257-t21l6i3f 314 9 cultures culture NNS cord-001257-t21l6i3f 314 10 with with IN cord-001257-t21l6i3f 314 11 the the DT cord-001257-t21l6i3f 314 12 following follow VBG cord-001257-t21l6i3f 314 13 density density NN cord-001257-t21l6i3f 314 14 : : : cord-001257-t21l6i3f 314 15 0.15 0.15 CD cord-001257-t21l6i3f 314 16 OD OD NNP cord-001257-t21l6i3f 314 17 600 600 CD cord-001257-t21l6i3f 314 18 for for IN cord-001257-t21l6i3f 314 19 pGD pGD NNP cord-001257-t21l6i3f 314 20 - - HYPH cord-001257-t21l6i3f 314 21 CNV CNV NNP cord-001257-t21l6i3f 314 22 , , , cord-001257-t21l6i3f 314 23 0.15 0.15 CD cord-001257-t21l6i3f 314 24 for for IN cord-001257-t21l6i3f 314 25 pGD pgd NN cord-001257-t21l6i3f 314 26 - - HYPH cord-001257-t21l6i3f 314 27 p19 p19 NN cord-001257-t21l6i3f 314 28 and and CC cord-001257-t21l6i3f 314 29 0.7 0.7 CD cord-001257-t21l6i3f 314 30 for for IN cord-001257-t21l6i3f 314 31 one one CD cord-001257-t21l6i3f 314 32 of of IN cord-001257-t21l6i3f 314 33 pGD pgd NN cord-001257-t21l6i3f 314 34 - - : cord-001257-t21l6i3f 314 35 RHx RHx NNS cord-001257-t21l6i3f 314 36 ( ( -LRB- cord-001257-t21l6i3f 314 37 or or CC cord-001257-t21l6i3f 314 38 empty empty JJ cord-001257-t21l6i3f 314 39 pGD pGD NNS cord-001257-t21l6i3f 314 40 ) ) -RRB- cord-001257-t21l6i3f 314 41 or or CC cord-001257-t21l6i3f 314 42 0.35 0.35 CD cord-001257-t21l6i3f 314 43 for for IN cord-001257-t21l6i3f 314 44 each each DT cord-001257-t21l6i3f 314 45 of of IN cord-001257-t21l6i3f 314 46 pGD pgd NN cord-001257-t21l6i3f 314 47 - - : cord-001257-t21l6i3f 314 48 RHx RHx NNS cord-001257-t21l6i3f 314 49 when when WRB cord-001257-t21l6i3f 314 50 combination combination NN cord-001257-t21l6i3f 314 51 of of IN cord-001257-t21l6i3f 314 52 two two CD cord-001257-t21l6i3f 314 53 was be VBD cord-001257-t21l6i3f 314 54 applied apply VBN cord-001257-t21l6i3f 314 55 to to IN cord-001257-t21l6i3f 314 56 the the DT cord-001257-t21l6i3f 314 57 same same JJ cord-001257-t21l6i3f 314 58 leaf leaf NN cord-001257-t21l6i3f 314 59 . . . cord-001257-t21l6i3f 315 1 Plant plant NN cord-001257-t21l6i3f 315 2 samples sample NNS cord-001257-t21l6i3f 315 3 from from IN cord-001257-t21l6i3f 315 4 infiltrated infiltrate VBN cord-001257-t21l6i3f 315 5 leaves leave NNS cord-001257-t21l6i3f 315 6 were be VBD cord-001257-t21l6i3f 315 7 taken take VBN cord-001257-t21l6i3f 315 8 60 60 CD cord-001257-t21l6i3f 315 9 hours hour NNS cord-001257-t21l6i3f 315 10 after after IN cord-001257-t21l6i3f 315 11 infection infection NN cord-001257-t21l6i3f 315 12 . . . cord-001257-t21l6i3f 316 1 RNA RNA NNP cord-001257-t21l6i3f 316 2 was be VBD cord-001257-t21l6i3f 316 3 isolated isolate VBN cord-001257-t21l6i3f 316 4 and and CC cord-001257-t21l6i3f 316 5 Northern northern JJ cord-001257-t21l6i3f 316 6 blot blot NN cord-001257-t21l6i3f 316 7 analysis analysis NN cord-001257-t21l6i3f 316 8 was be VBD cord-001257-t21l6i3f 316 9 performed perform VBN cord-001257-t21l6i3f 316 10 using use VBG cord-001257-t21l6i3f 316 11 previously previously RB cord-001257-t21l6i3f 316 12 described describe VBN cord-001257-t21l6i3f 316 13 [ [ -LRB- cord-001257-t21l6i3f 316 14 14 14 CD cord-001257-t21l6i3f 316 15 , , , cord-001257-t21l6i3f 316 16 95 95 CD cord-001257-t21l6i3f 316 17 ] ] -RRB- cord-001257-t21l6i3f 316 18 . . . cord-001257-t21l6i3f 317 1 For for IN cord-001257-t21l6i3f 317 2 selected select VBN cord-001257-t21l6i3f 317 3 samples sample NNS cord-001257-t21l6i3f 317 4 , , , cord-001257-t21l6i3f 317 5 proteins protein NNS cord-001257-t21l6i3f 317 6 were be VBD cord-001257-t21l6i3f 317 7 isolated isolate VBN cord-001257-t21l6i3f 317 8 and and CC cord-001257-t21l6i3f 317 9 total total JJ cord-001257-t21l6i3f 317 10 proteins protein NNS cord-001257-t21l6i3f 317 11 level level NN cord-001257-t21l6i3f 317 12 was be VBD cord-001257-t21l6i3f 317 13 adjusted adjust VBN cord-001257-t21l6i3f 317 14 based base VBN cord-001257-t21l6i3f 317 15 on on IN cord-001257-t21l6i3f 317 16 Coomassie Coomassie NNP cord-001257-t21l6i3f 317 17 - - HYPH cord-001257-t21l6i3f 317 18 blue blue JJ cord-001257-t21l6i3f 317 19 staining staining NN cord-001257-t21l6i3f 317 20 . . . cord-001257-t21l6i3f 318 1 For for IN cord-001257-t21l6i3f 318 2 Western western JJ cord-001257-t21l6i3f 318 3 blot blot NN cord-001257-t21l6i3f 318 4 analysis analysis NN cord-001257-t21l6i3f 318 5 , , , cord-001257-t21l6i3f 318 6 anti anti JJ cord-001257-t21l6i3f 318 7 - - JJ cord-001257-t21l6i3f 318 8 p33 p33 JJ cord-001257-t21l6i3f 318 9 antibody antibody NN cord-001257-t21l6i3f 318 10 was be VBD cord-001257-t21l6i3f 318 11 used use VBN cord-001257-t21l6i3f 318 12 ( ( -LRB- cord-001257-t21l6i3f 318 13 a a DT cord-001257-t21l6i3f 318 14 generous generous JJ cord-001257-t21l6i3f 318 15 gift gift NN cord-001257-t21l6i3f 318 16 of of IN cord-001257-t21l6i3f 318 17 Herman Herman NNP cord-001257-t21l6i3f 318 18 Scholthof Scholthof NNP cord-001257-t21l6i3f 318 19 , , , cord-001257-t21l6i3f 318 20 Texas Texas NNP cord-001257-t21l6i3f 318 21 AM AM NNP cord-001257-t21l6i3f 318 22 University University NNP cord-001257-t21l6i3f 318 23 ) ) -RRB- cord-001257-t21l6i3f 318 24 . . . cord-001257-t21l6i3f 319 1 Pictures picture NNS cord-001257-t21l6i3f 319 2 of of IN cord-001257-t21l6i3f 319 3 infected infected JJ cord-001257-t21l6i3f 319 4 plants plant NNS cord-001257-t21l6i3f 319 5 were be VBD cord-001257-t21l6i3f 319 6 taken take VBN cord-001257-t21l6i3f 319 7 7 7 CD cord-001257-t21l6i3f 319 8 days day NNS cord-001257-t21l6i3f 319 9 after after IN cord-001257-t21l6i3f 319 10 agroinfiltration agroinfiltration NN cord-001257-t21l6i3f 319 11 . . . cord-001257-t21l6i3f 320 1 Recombinant recombinant JJ cord-001257-t21l6i3f 320 2 MBP MBP NNP cord-001257-t21l6i3f 320 3 - - HYPH cord-001257-t21l6i3f 320 4 tagged tag VBN cord-001257-t21l6i3f 320 5 helicase helicase NN cord-001257-t21l6i3f 320 6 proteins protein NNS cord-001257-t21l6i3f 320 7 , , , cord-001257-t21l6i3f 320 8 the the DT cord-001257-t21l6i3f 320 9 MBP MBP NNP cord-001257-t21l6i3f 320 10 - - HYPH cord-001257-t21l6i3f 320 11 tagged tag VBN cord-001257-t21l6i3f 320 12 TBSV TBSV NNP cord-001257-t21l6i3f 320 13 p33 p33 NNP cord-001257-t21l6i3f 320 14 and and CC cord-001257-t21l6i3f 320 15 p92 p92 NN cord-001257-t21l6i3f 320 16 replication replication NN cord-001257-t21l6i3f 320 17 proteins protein NNS cord-001257-t21l6i3f 320 18 and and CC cord-001257-t21l6i3f 320 19 several several JJ cord-001257-t21l6i3f 320 20 truncated truncate VBN cord-001257-t21l6i3f 320 21 MBP MBP NNP cord-001257-t21l6i3f 320 22 - - HYPH cord-001257-t21l6i3f 320 23 tagged tag VBN cord-001257-t21l6i3f 320 24 p33C p33C NNP cord-001257-t21l6i3f 320 25 derivatives derivative NNS cord-001257-t21l6i3f 320 26 ( ( -LRB- cord-001257-t21l6i3f 320 27 described describe VBN cord-001257-t21l6i3f 320 28 earlier early RBR cord-001257-t21l6i3f 320 29 ) ) -RRB- cord-001257-t21l6i3f 320 30 were be VBD cord-001257-t21l6i3f 320 31 expressed express VBN cord-001257-t21l6i3f 320 32 in in IN cord-001257-t21l6i3f 320 33 E. E. NNP cord-001257-t21l6i3f 320 34 coli coli NNS cord-001257-t21l6i3f 320 35 and and CC cord-001257-t21l6i3f 320 36 purified purify VBN cord-001257-t21l6i3f 320 37 as as IN cord-001257-t21l6i3f 320 38 published publish VBN cord-001257-t21l6i3f 320 39 earlier early RBR cord-001257-t21l6i3f 320 40 with with IN cord-001257-t21l6i3f 320 41 modifications modification NNS cord-001257-t21l6i3f 320 42 [ [ -LRB- cord-001257-t21l6i3f 320 43 72 72 CD cord-001257-t21l6i3f 320 44 , , , cord-001257-t21l6i3f 320 45 96 96 CD cord-001257-t21l6i3f 320 46 ] ] -RRB- cord-001257-t21l6i3f 320 47 . . . cord-001257-t21l6i3f 321 1 Briefly briefly RB cord-001257-t21l6i3f 321 2 , , , cord-001257-t21l6i3f 321 3 the the DT cord-001257-t21l6i3f 321 4 expression expression NN cord-001257-t21l6i3f 321 5 plasmids plasmid NNS cord-001257-t21l6i3f 321 6 were be VBD cord-001257-t21l6i3f 321 7 transformed transform VBN cord-001257-t21l6i3f 321 8 into into IN cord-001257-t21l6i3f 321 9 E. E. NNP cord-001257-t21l6i3f 321 10 coli coli NNS cord-001257-t21l6i3f 321 11 strain strain VBP cord-001257-t21l6i3f 321 12 BL21 BL21 NNP cord-001257-t21l6i3f 321 13 ( ( -LRB- cord-001257-t21l6i3f 321 14 DE3 de3 NN cord-001257-t21l6i3f 321 15 ) ) -RRB- cord-001257-t21l6i3f 321 16 CodonPlus CodonPlus NNP cord-001257-t21l6i3f 321 17 . . . cord-001257-t21l6i3f 322 1 Protein protein NN cord-001257-t21l6i3f 322 2 expression expression NN cord-001257-t21l6i3f 322 3 of of IN cord-001257-t21l6i3f 322 4 the the DT cord-001257-t21l6i3f 322 5 selected select VBN cord-001257-t21l6i3f 322 6 helicase helicase NN cord-001257-t21l6i3f 322 7 proteins protein NNS cord-001257-t21l6i3f 322 8 was be VBD cord-001257-t21l6i3f 322 9 induced induce VBN cord-001257-t21l6i3f 322 10 by by IN cord-001257-t21l6i3f 322 11 isopropyl isopropyl NN cord-001257-t21l6i3f 322 12 - - HYPH cord-001257-t21l6i3f 322 13 b b NN cord-001257-t21l6i3f 322 14 - - HYPH cord-001257-t21l6i3f 322 15 D d JJ cord-001257-t21l6i3f 322 16 - - HYPH cord-001257-t21l6i3f 322 17 thiogalactopyranoside thiogalactopyranoside NN cord-001257-t21l6i3f 322 18 ( ( -LRB- cord-001257-t21l6i3f 322 19 IPTG IPTG NNP cord-001257-t21l6i3f 322 20 ) ) -RRB- cord-001257-t21l6i3f 322 21 for for IN cord-001257-t21l6i3f 322 22 8 8 CD cord-001257-t21l6i3f 322 23 h h NN cord-001257-t21l6i3f 322 24 at at IN cord-001257-t21l6i3f 322 25 23uC 23uc CD cord-001257-t21l6i3f 322 26 and and CC cord-001257-t21l6i3f 322 27 in in IN cord-001257-t21l6i3f 322 28 the the DT cord-001257-t21l6i3f 322 29 case case NN cord-001257-t21l6i3f 322 30 of of IN cord-001257-t21l6i3f 322 31 viral viral JJ cord-001257-t21l6i3f 322 32 proteins protein NNS cord-001257-t21l6i3f 322 33 p33 p33 CD cord-001257-t21l6i3f 322 34 and and CC cord-001257-t21l6i3f 322 35 p92 p92 NN cord-001257-t21l6i3f 322 36 at at IN cord-001257-t21l6i3f 322 37 16uC. 16uc. CD cord-001257-t21l6i3f 322 38 After after IN cord-001257-t21l6i3f 322 39 the the DT cord-001257-t21l6i3f 322 40 collection collection NN cord-001257-t21l6i3f 322 41 of of IN cord-001257-t21l6i3f 322 42 cells cell NNS cord-001257-t21l6i3f 322 43 by by IN cord-001257-t21l6i3f 322 44 centrifugation centrifugation NN cord-001257-t21l6i3f 322 45 ( ( -LRB- cord-001257-t21l6i3f 322 46 5,000 5,000 CD cord-001257-t21l6i3f 322 47 rpm rpm NN cord-001257-t21l6i3f 322 48 for for IN cord-001257-t21l6i3f 322 49 5 5 CD cord-001257-t21l6i3f 322 50 min min NNP cord-001257-t21l6i3f 322 51 ) ) -RRB- cord-001257-t21l6i3f 322 52 , , , cord-001257-t21l6i3f 322 53 the the DT cord-001257-t21l6i3f 322 54 cells cell NNS cord-001257-t21l6i3f 322 55 were be VBD cord-001257-t21l6i3f 322 56 resuspended resuspend VBN cord-001257-t21l6i3f 322 57 and and CC cord-001257-t21l6i3f 322 58 sonicated sonicate VBN cord-001257-t21l6i3f 322 59 in in IN cord-001257-t21l6i3f 322 60 low low JJ cord-001257-t21l6i3f 322 61 - - HYPH cord-001257-t21l6i3f 322 62 salt salt NN cord-001257-t21l6i3f 322 63 column column NN cord-001257-t21l6i3f 322 64 buffer buffer NN cord-001257-t21l6i3f 322 65 ( ( -LRB- cord-001257-t21l6i3f 322 66 30 30 CD cord-001257-t21l6i3f 322 67 mM mm CD cord-001257-t21l6i3f 322 68 HEPES HEPES NNP cord-001257-t21l6i3f 322 69 - - HYPH cord-001257-t21l6i3f 322 70 KOH KOH NNP cord-001257-t21l6i3f 322 71 pH pH NNP cord-001257-t21l6i3f 322 72 7.4 7.4 CD cord-001257-t21l6i3f 322 73 , , , cord-001257-t21l6i3f 322 74 25 25 CD cord-001257-t21l6i3f 322 75 mM mm CD cord-001257-t21l6i3f 322 76 NaCl nacl NN cord-001257-t21l6i3f 322 77 , , , cord-001257-t21l6i3f 322 78 1 1 CD cord-001257-t21l6i3f 322 79 mM mm CD cord-001257-t21l6i3f 322 80 EDTA EDTA NNP cord-001257-t21l6i3f 322 81 , , , cord-001257-t21l6i3f 322 82 10 10 CD cord-001257-t21l6i3f 322 83 mM mm CD cord-001257-t21l6i3f 322 84 b b NN cord-001257-t21l6i3f 322 85 - - HYPH cord-001257-t21l6i3f 322 86 mercaptoethanol mercaptoethanol NNP cord-001257-t21l6i3f 322 87 ) ) -RRB- cord-001257-t21l6i3f 322 88 . . . cord-001257-t21l6i3f 323 1 To to TO cord-001257-t21l6i3f 323 2 remove remove VB cord-001257-t21l6i3f 323 3 cells cell NNS cord-001257-t21l6i3f 323 4 debris debris NN cord-001257-t21l6i3f 323 5 , , , cord-001257-t21l6i3f 323 6 the the DT cord-001257-t21l6i3f 323 7 lysate lysate NN cord-001257-t21l6i3f 323 8 was be VBD cord-001257-t21l6i3f 323 9 centrifuged centrifuge VBN cord-001257-t21l6i3f 323 10 at at IN cord-001257-t21l6i3f 323 11 14,000 14,000 CD cord-001257-t21l6i3f 323 12 rpm rpm NN cord-001257-t21l6i3f 323 13 for for IN cord-001257-t21l6i3f 323 14 5 5 CD cord-001257-t21l6i3f 323 15 min min NN cord-001257-t21l6i3f 323 16 , , , cord-001257-t21l6i3f 323 17 followed follow VBN cord-001257-t21l6i3f 323 18 by by IN cord-001257-t21l6i3f 323 19 supernatant supernatant JJ cord-001257-t21l6i3f 323 20 incubation incubation NN cord-001257-t21l6i3f 323 21 with with IN cord-001257-t21l6i3f 323 22 amylose amylose JJ cord-001257-t21l6i3f 323 23 resin resin NN cord-001257-t21l6i3f 323 24 ( ( -LRB- cord-001257-t21l6i3f 323 25 NEB NEB NNP cord-001257-t21l6i3f 323 26 ) ) -RRB- cord-001257-t21l6i3f 323 27 for for IN cord-001257-t21l6i3f 323 28 15 15 CD cord-001257-t21l6i3f 323 29 min min NN cord-001257-t21l6i3f 323 30 at at IN cord-001257-t21l6i3f 323 31 4uC. 4uc. CD cord-001257-t21l6i3f 324 1 After after IN cord-001257-t21l6i3f 324 2 careful careful JJ cord-001257-t21l6i3f 324 3 washing washing NN cord-001257-t21l6i3f 324 4 of of IN cord-001257-t21l6i3f 324 5 the the DT cord-001257-t21l6i3f 324 6 columns column NNS cord-001257-t21l6i3f 324 7 , , , cord-001257-t21l6i3f 324 8 the the DT cord-001257-t21l6i3f 324 9 proteins protein NNS cord-001257-t21l6i3f 324 10 were be VBD cord-001257-t21l6i3f 324 11 eluted elute VBN cord-001257-t21l6i3f 324 12 with with IN cord-001257-t21l6i3f 324 13 MBP MBP NNP cord-001257-t21l6i3f 324 14 - - HYPH cord-001257-t21l6i3f 324 15 elution elution NN cord-001257-t21l6i3f 324 16 buffer buffer NN cord-001257-t21l6i3f 324 17 [ [ -LRB- cord-001257-t21l6i3f 324 18 column column NN cord-001257-t21l6i3f 324 19 buffer buffer NN cord-001257-t21l6i3f 324 20 containing contain VBG cord-001257-t21l6i3f 324 21 0.18 0.18 CD cord-001257-t21l6i3f 324 22 % % NN cord-001257-t21l6i3f 324 23 ( ( -LRB- cord-001257-t21l6i3f 324 24 W w NN cord-001257-t21l6i3f 324 25 / / SYM cord-001257-t21l6i3f 324 26 V v NN cord-001257-t21l6i3f 324 27 ) ) -RRB- cord-001257-t21l6i3f 324 28 maltose maltose NN cord-001257-t21l6i3f 324 29 ] ] -RRB- cord-001257-t21l6i3f 324 30 . . . cord-001257-t21l6i3f 325 1 Purification purification NN cord-001257-t21l6i3f 325 2 of of IN cord-001257-t21l6i3f 325 3 His -PRON- PRP$ cord-001257-t21l6i3f 325 4 6 6 CD cord-001257-t21l6i3f 325 5 -tagged -tagged CD cord-001257-t21l6i3f 325 6 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 325 7 and and CC cord-001257-t21l6i3f 325 8 His -PRON- PRP$ cord-001257-t21l6i3f 325 9 6 6 CD cord-001257-t21l6i3f 325 10 -AtRH5 -atrh5 NN cord-001257-t21l6i3f 326 1 ( ( -LRB- cord-001257-t21l6i3f 326 2 using use VBG cord-001257-t21l6i3f 326 3 plasmids plasmid NNS cord-001257-t21l6i3f 326 4 pET30-RH2 pET30-RH2 NNP cord-001257-t21l6i3f 326 5 or or CC cord-001257-t21l6i3f 326 6 pET30-RH5 pET30-RH5 NNP cord-001257-t21l6i3f 326 7 ) ) -RRB- cord-001257-t21l6i3f 326 8 was be VBD cord-001257-t21l6i3f 326 9 carried carry VBN cord-001257-t21l6i3f 326 10 out out RP cord-001257-t21l6i3f 326 11 using use VBG cord-001257-t21l6i3f 326 12 ProBond ProBond NNP cord-001257-t21l6i3f 326 13 ( ( -LRB- cord-001257-t21l6i3f 326 14 Invitrogen Invitrogen NNP cord-001257-t21l6i3f 326 15 ) ) -RRB- cord-001257-t21l6i3f 326 16 resin resin NN cord-001257-t21l6i3f 326 17 ( ( -LRB- cord-001257-t21l6i3f 326 18 washed wash VBN cord-001257-t21l6i3f 326 19 with with IN cord-001257-t21l6i3f 326 20 column column NN cord-001257-t21l6i3f 326 21 buffer buffer NN cord-001257-t21l6i3f 326 22 , , , cord-001257-t21l6i3f 326 23 containing contain VBG cord-001257-t21l6i3f 326 24 60 60 CD cord-001257-t21l6i3f 326 25 mM mM NNP cord-001257-t21l6i3f 326 26 Imidazole Imidazole NNP cord-001257-t21l6i3f 326 27 and and CC cord-001257-t21l6i3f 326 28 eluted elute VBN cord-001257-t21l6i3f 326 29 with with IN cord-001257-t21l6i3f 326 30 column column NN cord-001257-t21l6i3f 326 31 buffer buffer NN cord-001257-t21l6i3f 326 32 [ [ -LRB- cord-001257-t21l6i3f 326 33 lacking lack VBG cord-001257-t21l6i3f 326 34 b b NN cord-001257-t21l6i3f 326 35 - - HYPH cord-001257-t21l6i3f 326 36 mercaptoethanol mercaptoethanol NN cord-001257-t21l6i3f 326 37 ] ] -RRB- cord-001257-t21l6i3f 326 38 , , , cord-001257-t21l6i3f 326 39 containing contain VBG cord-001257-t21l6i3f 326 40 1 1 CD cord-001257-t21l6i3f 326 41 M M NNP cord-001257-t21l6i3f 326 42 Imidazole Imidazole NNP cord-001257-t21l6i3f 326 43 ) ) -RRB- cord-001257-t21l6i3f 326 44 , , , cord-001257-t21l6i3f 326 45 following follow VBG cord-001257-t21l6i3f 326 46 otherwise otherwise RB cord-001257-t21l6i3f 326 47 the the DT cord-001257-t21l6i3f 326 48 same same JJ cord-001257-t21l6i3f 326 49 protocol protocol NN cord-001257-t21l6i3f 326 50 as as IN cord-001257-t21l6i3f 326 51 for for IN cord-001257-t21l6i3f 326 52 the the DT cord-001257-t21l6i3f 326 53 MBP MBP NNP cord-001257-t21l6i3f 326 54 - - HYPH cord-001257-t21l6i3f 326 55 tagged tag VBN cord-001257-t21l6i3f 326 56 proteins protein NNS cord-001257-t21l6i3f 326 57 . . . cord-001257-t21l6i3f 327 1 Purified purify VBN cord-001257-t21l6i3f 327 2 proteins protein NNS cord-001257-t21l6i3f 327 3 were be VBD cord-001257-t21l6i3f 327 4 aliquoted aliquote VBN cord-001257-t21l6i3f 327 5 and and CC cord-001257-t21l6i3f 327 6 stored store VBN cord-001257-t21l6i3f 327 7 at at IN cord-001257-t21l6i3f 327 8 280uC. 280uc. CD cord-001257-t21l6i3f 327 9 Proteins protein NNS cord-001257-t21l6i3f 327 10 used use VBN cord-001257-t21l6i3f 327 11 for for IN cord-001257-t21l6i3f 327 12 the the DT cord-001257-t21l6i3f 327 13 replication replication NN cord-001257-t21l6i3f 327 14 assays assay NNS cord-001257-t21l6i3f 327 15 were be VBD cord-001257-t21l6i3f 327 16 at at RB cord-001257-t21l6i3f 327 17 least least RBS cord-001257-t21l6i3f 327 18 95 95 CD cord-001257-t21l6i3f 327 19 % % NN cord-001257-t21l6i3f 327 20 pure pure JJ cord-001257-t21l6i3f 327 21 , , , cord-001257-t21l6i3f 327 22 as as IN cord-001257-t21l6i3f 327 23 determined determine VBN cord-001257-t21l6i3f 327 24 by by IN cord-001257-t21l6i3f 327 25 SDS SDS NNP cord-001257-t21l6i3f 327 26 - - HYPH cord-001257-t21l6i3f 327 27 PAGE PAGE NNP cord-001257-t21l6i3f 327 28 ( ( -LRB- cord-001257-t21l6i3f 327 29 not not RB cord-001257-t21l6i3f 327 30 shown show VBN cord-001257-t21l6i3f 327 31 ) ) -RRB- cord-001257-t21l6i3f 327 32 . . . cord-001257-t21l6i3f 328 1 For for IN cord-001257-t21l6i3f 328 2 in in IN cord-001257-t21l6i3f 328 3 vitro vitro FW cord-001257-t21l6i3f 328 4 pull pull VB cord-001257-t21l6i3f 328 5 - - HYPH cord-001257-t21l6i3f 328 6 down down RP cord-001257-t21l6i3f 328 7 assay assay NN cord-001257-t21l6i3f 328 8 , , , cord-001257-t21l6i3f 328 9 purified purify VBD cord-001257-t21l6i3f 328 10 His -PRON- PRP$ cord-001257-t21l6i3f 328 11 6 6 CD cord-001257-t21l6i3f 328 12 -tagged -tagged CD cord-001257-t21l6i3f 328 13 helicase helicase NN cord-001257-t21l6i3f 328 14 proteins protein NNS cord-001257-t21l6i3f 328 15 ( ( -LRB- cord-001257-t21l6i3f 328 16 200 200 CD cord-001257-t21l6i3f 328 17 mg mg NNS cord-001257-t21l6i3f 328 18 ) ) -RRB- cord-001257-t21l6i3f 328 19 were be VBD cord-001257-t21l6i3f 328 20 loaded load VBN cord-001257-t21l6i3f 328 21 onto onto IN cord-001257-t21l6i3f 328 22 MBP MBP NNP cord-001257-t21l6i3f 328 23 columns column NNS cord-001257-t21l6i3f 328 24 , , , cord-001257-t21l6i3f 328 25 containing contain VBG cord-001257-t21l6i3f 328 26 bound bind VBN cord-001257-t21l6i3f 328 27 MBP MBP NNP cord-001257-t21l6i3f 328 28 - - HYPH cord-001257-t21l6i3f 328 29 tagged tag VBN cord-001257-t21l6i3f 328 30 p33C p33C NNP cord-001257-t21l6i3f 328 31 derivatives derivative NNS cord-001257-t21l6i3f 328 32 and and CC cord-001257-t21l6i3f 328 33 incubated incubate VBN cord-001257-t21l6i3f 328 34 with with IN cord-001257-t21l6i3f 328 35 mixing mix VBG cord-001257-t21l6i3f 328 36 for for IN cord-001257-t21l6i3f 328 37 25 25 CD cord-001257-t21l6i3f 328 38 min min NN cord-001257-t21l6i3f 328 39 at at IN cord-001257-t21l6i3f 328 40 4uC 4uc CD cord-001257-t21l6i3f 328 41 [ [ -LRB- cord-001257-t21l6i3f 328 42 30 30 CD cord-001257-t21l6i3f 328 43 ] ] -RRB- cord-001257-t21l6i3f 328 44 . . . cord-001257-t21l6i3f 329 1 The the DT cord-001257-t21l6i3f 329 2 columns column NNS cord-001257-t21l6i3f 329 3 were be VBD cord-001257-t21l6i3f 329 4 washed wash VBN cord-001257-t21l6i3f 329 5 three three CD cord-001257-t21l6i3f 329 6 times time NNS cord-001257-t21l6i3f 329 7 with with IN cord-001257-t21l6i3f 329 8 cold cold JJ cord-001257-t21l6i3f 329 9 column column NN cord-001257-t21l6i3f 329 10 buffer buffer NN cord-001257-t21l6i3f 329 11 and and CC cord-001257-t21l6i3f 329 12 the the DT cord-001257-t21l6i3f 329 13 bound bind VBN cord-001257-t21l6i3f 329 14 protein protein NN cord-001257-t21l6i3f 329 15 complexes complex NNS cord-001257-t21l6i3f 329 16 were be VBD cord-001257-t21l6i3f 329 17 eluted elute VBN cord-001257-t21l6i3f 329 18 with with IN cord-001257-t21l6i3f 329 19 MBP MBP NNP cord-001257-t21l6i3f 329 20 - - HYPH cord-001257-t21l6i3f 329 21 elution elution NN cord-001257-t21l6i3f 329 22 buffer buffer NN cord-001257-t21l6i3f 329 23 . . . cord-001257-t21l6i3f 330 1 The the DT cord-001257-t21l6i3f 330 2 eluates eluate NNS cord-001257-t21l6i3f 330 3 were be VBD cord-001257-t21l6i3f 330 4 analyzed analyze VBN cord-001257-t21l6i3f 330 5 for for IN cord-001257-t21l6i3f 330 6 the the DT cord-001257-t21l6i3f 330 7 presence presence NN cord-001257-t21l6i3f 330 8 of of IN cord-001257-t21l6i3f 330 9 His -PRON- PRP$ cord-001257-t21l6i3f 330 10 6 6 CD cord-001257-t21l6i3f 330 11 -tagged -tagged CD cord-001257-t21l6i3f 330 12 proteins protein NNS cord-001257-t21l6i3f 330 13 by by IN cord-001257-t21l6i3f 330 14 SDS SDS NNP cord-001257-t21l6i3f 330 15 - - HYPH cord-001257-t21l6i3f 330 16 PAGE PAGE NNP cord-001257-t21l6i3f 330 17 , , , cord-001257-t21l6i3f 330 18 followed follow VBN cord-001257-t21l6i3f 330 19 by by IN cord-001257-t21l6i3f 330 20 Coomassie Coomassie NNP cord-001257-t21l6i3f 330 21 blue blue JJ cord-001257-t21l6i3f 330 22 staining staining NN cord-001257-t21l6i3f 330 23 or or CC cord-001257-t21l6i3f 330 24 Western western JJ cord-001257-t21l6i3f 330 25 blotting blotting NN cord-001257-t21l6i3f 330 26 with with IN cord-001257-t21l6i3f 330 27 an an DT cord-001257-t21l6i3f 330 28 anti anti JJ cord-001257-t21l6i3f 330 29 - - JJ cord-001257-t21l6i3f 330 30 His -PRON- PRP$ cord-001257-t21l6i3f 330 31 antibody antibody NN cord-001257-t21l6i3f 330 32 . . . cord-001257-t21l6i3f 331 1 PCR PCR NNP cord-001257-t21l6i3f 331 2 products product NNS cord-001257-t21l6i3f 331 3 for for IN cord-001257-t21l6i3f 331 4 ' ' '' cord-001257-t21l6i3f 331 5 ' ' '' cord-001257-t21l6i3f 331 6 + + CC cord-001257-t21l6i3f 331 7 bridge bridge NN cord-001257-t21l6i3f 331 8 ' ' '' cord-001257-t21l6i3f 331 9 ' ' '' cord-001257-t21l6i3f 331 10 and and CC cord-001257-t21l6i3f 331 11 ' ' '' cord-001257-t21l6i3f 331 12 ' ' `` cord-001257-t21l6i3f 331 13 Dbridge Dbridge NNP cord-001257-t21l6i3f 331 14 ' ' '' cord-001257-t21l6i3f 331 15 ' ' '' cord-001257-t21l6i3f 331 16 constructs construct NNS cord-001257-t21l6i3f 331 17 ( ( -LRB- cord-001257-t21l6i3f 331 18 Fig Fig NNP cord-001257-t21l6i3f 331 19 . . . cord-001257-t21l6i3f 331 20 3E 3E NNP cord-001257-t21l6i3f 331 21 ) ) -RRB- cord-001257-t21l6i3f 331 22 were be VBD cord-001257-t21l6i3f 331 23 prepared prepare VBN cord-001257-t21l6i3f 331 24 as as IN cord-001257-t21l6i3f 331 25 follows follow VBZ cord-001257-t21l6i3f 331 26 : : : cord-001257-t21l6i3f 332 1 pGBK pGBK NNP cord-001257-t21l6i3f 333 1 -HIS -HIS -LRB- cord-001257-t21l6i3f 333 2 - - : cord-001257-t21l6i3f 333 3 Cup Cup NNP cord-001257-t21l6i3f 333 4 - - HYPH cord-001257-t21l6i3f 333 5 Flag33 Flag33 NNP cord-001257-t21l6i3f 333 6 / / SYM cord-001257-t21l6i3f 333 7 Gal Gal NNP cord-001257-t21l6i3f 333 8 - - HYPH cord-001257-t21l6i3f 333 9 DI-72 DI-72 NNP cord-001257-t21l6i3f 333 10 was be VBD cord-001257-t21l6i3f 333 11 used use VBN cord-001257-t21l6i3f 333 12 as as IN cord-001257-t21l6i3f 333 13 a a DT cord-001257-t21l6i3f 333 14 template template NN cord-001257-t21l6i3f 333 15 for for IN cord-001257-t21l6i3f 333 16 PCR PCR NNP cord-001257-t21l6i3f 333 17 with with IN cord-001257-t21l6i3f 333 18 primer primer NN cord-001257-t21l6i3f 333 19 pairs pair NNS cord-001257-t21l6i3f 333 20 # # $ cord-001257-t21l6i3f 333 21 5480 5480 CD cord-001257-t21l6i3f 333 22 and and CC cord-001257-t21l6i3f 333 23 # # $ cord-001257-t21l6i3f 333 24 5481 5481 CD cord-001257-t21l6i3f 333 25 , , , cord-001257-t21l6i3f 333 26 or or CC cord-001257-t21l6i3f 333 27 # # $ cord-001257-t21l6i3f 333 28 5480 5480 CD cord-001257-t21l6i3f 333 29 and and CC cord-001257-t21l6i3f 333 30 # # $ cord-001257-t21l6i3f 333 31 5482 5482 CD cord-001257-t21l6i3f 333 32 , , , cord-001257-t21l6i3f 333 33 respectively respectively RB cord-001257-t21l6i3f 333 34 . . . cord-001257-t21l6i3f 334 1 The the DT cord-001257-t21l6i3f 334 2 generated generate VBN cord-001257-t21l6i3f 334 3 PCR PCR NNP cord-001257-t21l6i3f 334 4 products product NNS cord-001257-t21l6i3f 334 5 were be VBD cord-001257-t21l6i3f 334 6 used use VBN cord-001257-t21l6i3f 334 7 to to TO cord-001257-t21l6i3f 334 8 obtain obtain VB cord-001257-t21l6i3f 334 9 + + CC cord-001257-t21l6i3f 334 10 bridge bridge NN cord-001257-t21l6i3f 334 11 RNA RNA NNP cord-001257-t21l6i3f 334 12 ( ( -LRB- cord-001257-t21l6i3f 334 13 86 86 CD cord-001257-t21l6i3f 334 14 nt nt RB cord-001257-t21l6i3f 334 15 in in IN cord-001257-t21l6i3f 334 16 length length NN cord-001257-t21l6i3f 334 17 ) ) -RRB- cord-001257-t21l6i3f 334 18 or or CC cord-001257-t21l6i3f 334 19 Dbridge Dbridge NNP cord-001257-t21l6i3f 334 20 RNA RNA NNP cord-001257-t21l6i3f 334 21 ( ( -LRB- cord-001257-t21l6i3f 334 22 73 73 CD cord-001257-t21l6i3f 334 23 nt nt RB cord-001257-t21l6i3f 334 24 in in IN cord-001257-t21l6i3f 334 25 length length NN cord-001257-t21l6i3f 334 26 ) ) -RRB- cord-001257-t21l6i3f 334 27 , , , cord-001257-t21l6i3f 334 28 each each DT cord-001257-t21l6i3f 334 29 starting start VBG cord-001257-t21l6i3f 334 30 from from IN cord-001257-t21l6i3f 334 31 position position NN cord-001257-t21l6i3f 334 32 368 368 CD cord-001257-t21l6i3f 334 33 in in IN cord-001257-t21l6i3f 334 34 DI-72 DI-72 NNP cord-001257-t21l6i3f 334 35 . . . cord-001257-t21l6i3f 335 1 The the DT cord-001257-t21l6i3f 335 2 RNA RNA NNP cord-001257-t21l6i3f 335 3 transcripts transcript NNS cord-001257-t21l6i3f 335 4 were be VBD cord-001257-t21l6i3f 335 5 synthesized synthesize VBN cord-001257-t21l6i3f 335 6 on on IN cord-001257-t21l6i3f 335 7 the the DT cord-001257-t21l6i3f 335 8 PCR PCR NNP cord-001257-t21l6i3f 335 9 templates template NNS cord-001257-t21l6i3f 335 10 using use VBG cord-001257-t21l6i3f 335 11 T7-based t7-based JJ cord-001257-t21l6i3f 335 12 transcription transcription NN cord-001257-t21l6i3f 335 13 [ [ -LRB- cord-001257-t21l6i3f 335 14 97 97 CD cord-001257-t21l6i3f 335 15 ] ] -RRB- cord-001257-t21l6i3f 335 16 . . . cord-001257-t21l6i3f 336 1 The the DT cord-001257-t21l6i3f 336 2 RNA RNA NNP cord-001257-t21l6i3f 336 3 transcripts transcript NNS cord-001257-t21l6i3f 336 4 used use VBN cord-001257-t21l6i3f 336 5 in in IN cord-001257-t21l6i3f 336 6 CFE CFE NNP cord-001257-t21l6i3f 336 7 - - HYPH cord-001257-t21l6i3f 336 8 based base VBN cord-001257-t21l6i3f 336 9 replication replication NN cord-001257-t21l6i3f 336 10 or or CC cord-001257-t21l6i3f 336 11 replicase replicase NN cord-001257-t21l6i3f 336 12 assays assay NNS cord-001257-t21l6i3f 336 13 were be VBD cord-001257-t21l6i3f 336 14 purified purify VBN cord-001257-t21l6i3f 336 15 as as IN cord-001257-t21l6i3f 336 16 described describe VBN cord-001257-t21l6i3f 336 17 earlier early RBR cord-001257-t21l6i3f 336 18 [ [ -LRB- cord-001257-t21l6i3f 336 19 97 97 CD cord-001257-t21l6i3f 336 20 ] ] -RRB- cord-001257-t21l6i3f 336 21 . . . cord-001257-t21l6i3f 337 1 The the DT cord-001257-t21l6i3f 337 2 32 32 CD cord-001257-t21l6i3f 337 3 P p NN cord-001257-t21l6i3f 337 4 - - HYPH cord-001257-t21l6i3f 337 5 labeled label VBN cord-001257-t21l6i3f 337 6 or or CC cord-001257-t21l6i3f 337 7 unlabeled unlabeled JJ cord-001257-t21l6i3f 337 8 four four CD cord-001257-t21l6i3f 337 9 separate separate JJ cord-001257-t21l6i3f 337 10 regions region NNS cord-001257-t21l6i3f 337 11 ( ( -LRB- cord-001257-t21l6i3f 337 12 RI RI NNP cord-001257-t21l6i3f 337 13 - - HYPH cord-001257-t21l6i3f 337 14 IV IV NNP cord-001257-t21l6i3f 337 15 , , , cord-001257-t21l6i3f 337 16 Fig Fig NNP cord-001257-t21l6i3f 337 17 . . . cord-001257-t21l6i3f 337 18 3A 3A NNP cord-001257-t21l6i3f 337 19 ) ) -RRB- cord-001257-t21l6i3f 337 20 and and CC cord-001257-t21l6i3f 337 21 the the DT cord-001257-t21l6i3f 337 22 full full JJ cord-001257-t21l6i3f 337 23 - - HYPH cord-001257-t21l6i3f 337 24 length length NN cord-001257-t21l6i3f 337 25 DI-72 DI-72 NNP cord-001257-t21l6i3f 337 26 ( ( -LRB- cord-001257-t21l6i3f 337 27 + + NN cord-001257-t21l6i3f 337 28 ) ) -RRB- cord-001257-t21l6i3f 337 29 and and CC cord-001257-t21l6i3f 337 30 ( ( -LRB- cord-001257-t21l6i3f 337 31 2 2 LS cord-001257-t21l6i3f 337 32 ) ) -RRB- cord-001257-t21l6i3f 337 33 RNAs rna NNS cord-001257-t21l6i3f 337 34 were be VBD cord-001257-t21l6i3f 337 35 produced produce VBN cord-001257-t21l6i3f 337 36 as as IN cord-001257-t21l6i3f 337 37 published publish VBN cord-001257-t21l6i3f 337 38 [ [ -LRB- cord-001257-t21l6i3f 337 39 72 72 CD cord-001257-t21l6i3f 337 40 ] ] -RRB- cord-001257-t21l6i3f 337 41 . . . cord-001257-t21l6i3f 338 1 Full full JJ cord-001257-t21l6i3f 338 2 - - HYPH cord-001257-t21l6i3f 338 3 length length NN cord-001257-t21l6i3f 338 4 FHV fhv NN cord-001257-t21l6i3f 338 5 - - HYPH cord-001257-t21l6i3f 338 6 derived derive VBN cord-001257-t21l6i3f 339 1 DI-634 DI-634 NNP cord-001257-t21l6i3f 339 2 ( ( -LRB- cord-001257-t21l6i3f 339 3 + + NN cord-001257-t21l6i3f 339 4 ) ) -RRB- cord-001257-t21l6i3f 340 1 or or CC cord-001257-t21l6i3f 340 2 ( ( -LRB- cord-001257-t21l6i3f 340 3 2)RNA 2)rna CD cord-001257-t21l6i3f 340 4 was be VBD cord-001257-t21l6i3f 340 5 produced produce VBN cord-001257-t21l6i3f 340 6 as as IN cord-001257-t21l6i3f 340 7 described describe VBN cord-001257-t21l6i3f 340 8 [ [ -LRB- cord-001257-t21l6i3f 340 9 30 30 CD cord-001257-t21l6i3f 340 10 ] ] -RRB- cord-001257-t21l6i3f 340 11 . . . cord-001257-t21l6i3f 341 1 The the DT cord-001257-t21l6i3f 341 2 amounts amount NNS cord-001257-t21l6i3f 341 3 of of IN cord-001257-t21l6i3f 341 4 transcripts transcript NNS cord-001257-t21l6i3f 341 5 were be VBD cord-001257-t21l6i3f 341 6 quantified quantify VBN cord-001257-t21l6i3f 341 7 by by IN cord-001257-t21l6i3f 341 8 UV uv NN cord-001257-t21l6i3f 341 9 spectrophotometer spectrophotometer NN cord-001257-t21l6i3f 341 10 ( ( -LRB- cord-001257-t21l6i3f 341 11 Beckman Beckman NNP cord-001257-t21l6i3f 341 12 ) ) -RRB- cord-001257-t21l6i3f 341 13 . . . cord-001257-t21l6i3f 342 1 Partial partial JJ cord-001257-t21l6i3f 342 2 dsRNA dsrna NN cord-001257-t21l6i3f 342 3 duplexes duplex NNS cord-001257-t21l6i3f 342 4 [ [ -LRB- cord-001257-t21l6i3f 342 5 ( ( -LRB- cord-001257-t21l6i3f 342 6 2)R124/(+)DI-72 2)r124/(+)di-72 CD cord-001257-t21l6i3f 343 1 and and CC cord-001257-t21l6i3f 343 2 ( ( -LRB- cord-001257-t21l6i3f 343 3 ( ( -LRB- cord-001257-t21l6i3f 343 4 2)R134/(+ 2)r134/(+ CD cord-001257-t21l6i3f 343 5 ) ) -RRB- cord-001257-t21l6i3f 343 6 DI-72 DI-72 NNP cord-001257-t21l6i3f 343 7 ] ] -RRB- cord-001257-t21l6i3f 344 1 for for IN cord-001257-t21l6i3f 344 2 in in FW cord-001257-t21l6i3f 344 3 vitro vitro FW cord-001257-t21l6i3f 344 4 replicase replicase NN cord-001257-t21l6i3f 344 5 assay assay NN cord-001257-t21l6i3f 344 6 were be VBD cord-001257-t21l6i3f 344 7 prepared prepare VBN cord-001257-t21l6i3f 344 8 as as IN cord-001257-t21l6i3f 344 9 follows follow VBZ cord-001257-t21l6i3f 344 10 : : : cord-001257-t21l6i3f 344 11 approximately approximately RB cord-001257-t21l6i3f 344 12 2 2 CD cord-001257-t21l6i3f 344 13 pmol pmol NN cord-001257-t21l6i3f 344 14 of of IN cord-001257-t21l6i3f 344 15 32 32 CD cord-001257-t21l6i3f 344 16 P p NN cord-001257-t21l6i3f 344 17 - - HYPH cord-001257-t21l6i3f 344 18 labeled label VBN cord-001257-t21l6i3f 344 19 ( ( -LRB- cord-001257-t21l6i3f 344 20 2)R134 2)r134 CD cord-001257-t21l6i3f 344 21 or or CC cord-001257-t21l6i3f 344 22 ( ( -LRB- cord-001257-t21l6i3f 344 23 2)R124 2)r124 CD cord-001257-t21l6i3f 344 24 were be VBD cord-001257-t21l6i3f 344 25 annealed anneal VBN cord-001257-t21l6i3f 344 26 to to IN cord-001257-t21l6i3f 344 27 unlabeled unlabeled JJ cord-001257-t21l6i3f 344 28 DI-72(+ DI-72(+ NNP cord-001257-t21l6i3f 344 29 ) ) -RRB- cord-001257-t21l6i3f 344 30 RNA RNA NNP cord-001257-t21l6i3f 344 31 in in IN cord-001257-t21l6i3f 344 32 STE STE NNP cord-001257-t21l6i3f 344 33 buffer buffer NN cord-001257-t21l6i3f 344 34 ( ( -LRB- cord-001257-t21l6i3f 344 35 10 10 CD cord-001257-t21l6i3f 344 36 mM mm CD cord-001257-t21l6i3f 344 37 TRIS TRIS NNP cord-001257-t21l6i3f 344 38 , , , cord-001257-t21l6i3f 344 39 pH pH NNP cord-001257-t21l6i3f 344 40 8.0 8.0 CD cord-001257-t21l6i3f 344 41 , , , cord-001257-t21l6i3f 344 42 1 1 CD cord-001257-t21l6i3f 344 43 mM mm CD cord-001257-t21l6i3f 344 44 EDTA EDTA NNP cord-001257-t21l6i3f 344 45 , , , cord-001257-t21l6i3f 344 46 and and CC cord-001257-t21l6i3f 344 47 100 100 CD cord-001257-t21l6i3f 344 48 m m NN cord-001257-t21l6i3f 344 49 M M NNP cord-001257-t21l6i3f 344 50 NaCl NaCl NNP cord-001257-t21l6i3f 344 51 ) ) -RRB- cord-001257-t21l6i3f 344 52 by by IN cord-001257-t21l6i3f 344 53 slowly slowly RB cord-001257-t21l6i3f 344 54 cooling cool VBG cord-001257-t21l6i3f 344 55 down down RP cord-001257-t21l6i3f 344 56 the the DT cord-001257-t21l6i3f 344 57 samples sample NNS cord-001257-t21l6i3f 344 58 ( ( -LRB- cord-001257-t21l6i3f 344 59 in in IN cord-001257-t21l6i3f 344 60 20 20 CD cord-001257-t21l6i3f 344 61 ml ml NNS cord-001257-t21l6i3f 344 62 ) ) -RRB- cord-001257-t21l6i3f 344 63 from from IN cord-001257-t21l6i3f 344 64 94uC 94uc CD cord-001257-t21l6i3f 344 65 to to IN cord-001257-t21l6i3f 344 66 25uC 25uc CD cord-001257-t21l6i3f 344 67 in in IN cord-001257-t21l6i3f 344 68 30 30 CD cord-001257-t21l6i3f 344 69 min min NN cord-001257-t21l6i3f 344 70 . . . cord-001257-t21l6i3f 345 1 Complete complete JJ cord-001257-t21l6i3f 345 2 DI-72 DI-72 NNP cord-001257-t21l6i3f 345 3 dsRNA dsrna NN cord-001257-t21l6i3f 345 4 duplexes duplex NNS cord-001257-t21l6i3f 345 5 were be VBD cord-001257-t21l6i3f 345 6 prepared prepare VBN cord-001257-t21l6i3f 345 7 using use VBG cord-001257-t21l6i3f 345 8 Replicator Replicator NNP cord-001257-t21l6i3f 345 9 RNAi RNAi NNP cord-001257-t21l6i3f 345 10 kit kit NN cord-001257-t21l6i3f 345 11 ( ( -LRB- cord-001257-t21l6i3f 345 12 Finnzymes Finnzymes NNP cord-001257-t21l6i3f 345 13 ) ) -RRB- cord-001257-t21l6i3f 345 14 . . . cord-001257-t21l6i3f 346 1 Briefly briefly RB cord-001257-t21l6i3f 346 2 , , , cord-001257-t21l6i3f 346 3 DI-72 DI-72 NNP cord-001257-t21l6i3f 346 4 ( ( -LRB- cord-001257-t21l6i3f 346 5 + + NN cord-001257-t21l6i3f 346 6 ) ) -RRB- cord-001257-t21l6i3f 346 7 -strand -strand CD cord-001257-t21l6i3f 346 8 RNA RNA NNP cord-001257-t21l6i3f 346 9 , , , cord-001257-t21l6i3f 346 10 which which WDT cord-001257-t21l6i3f 346 11 was be VBD cord-001257-t21l6i3f 346 12 synthesized synthesize VBN cord-001257-t21l6i3f 346 13 with with IN cord-001257-t21l6i3f 346 14 T7 t7 NN cord-001257-t21l6i3f 346 15 transcription transcription NN cord-001257-t21l6i3f 346 16 , , , cord-001257-t21l6i3f 346 17 was be VBD cord-001257-t21l6i3f 346 18 used use VBN cord-001257-t21l6i3f 346 19 as as IN cord-001257-t21l6i3f 346 20 a a DT cord-001257-t21l6i3f 346 21 template template NN cord-001257-t21l6i3f 346 22 for for IN cord-001257-t21l6i3f 346 23 synthesis synthesis NN cord-001257-t21l6i3f 346 24 of of IN cord-001257-t21l6i3f 346 25 DI-72 DI-72 NNP cord-001257-t21l6i3f 346 26 dsRNA dsrna NN cord-001257-t21l6i3f 346 27 by by IN cord-001257-t21l6i3f 346 28 Phi6 Phi6 NNP cord-001257-t21l6i3f 346 29 RNA RNA NNP cord-001257-t21l6i3f 346 30 polymerase polymerase NN cord-001257-t21l6i3f 346 31 in in IN cord-001257-t21l6i3f 346 32 vitro vitro FW cord-001257-t21l6i3f 346 33 . . . cord-001257-t21l6i3f 347 1 Purity purity NN cord-001257-t21l6i3f 347 2 of of IN cord-001257-t21l6i3f 347 3 dsRNAs dsRNAs NNP cord-001257-t21l6i3f 347 4 was be VBD cord-001257-t21l6i3f 347 5 tested test VBN cord-001257-t21l6i3f 347 6 with with IN cord-001257-t21l6i3f 347 7 agarose agarose JJ cord-001257-t21l6i3f 347 8 gel gel NN cord-001257-t21l6i3f 347 9 - - HYPH cord-001257-t21l6i3f 347 10 electrophoresis electrophoresis NN cord-001257-t21l6i3f 347 11 . . . cord-001257-t21l6i3f 348 1 Gel gel NN cord-001257-t21l6i3f 348 2 mobility mobility NN cord-001257-t21l6i3f 348 3 shift shift NN cord-001257-t21l6i3f 348 4 assay assay NN cord-001257-t21l6i3f 348 5 ( ( -LRB- cord-001257-t21l6i3f 348 6 EMSA EMSA NNP cord-001257-t21l6i3f 348 7 ) ) -RRB- cord-001257-t21l6i3f 348 8 and and CC cord-001257-t21l6i3f 348 9 strand strand NN cord-001257-t21l6i3f 348 10 separation separation NN cord-001257-t21l6i3f 348 11 assay assay NN cord-001257-t21l6i3f 348 12 EMSA EMSA NNP cord-001257-t21l6i3f 348 13 was be VBD cord-001257-t21l6i3f 348 14 performed perform VBN cord-001257-t21l6i3f 348 15 as as IN cord-001257-t21l6i3f 348 16 described describe VBN cord-001257-t21l6i3f 348 17 previously previously RB cord-001257-t21l6i3f 348 18 [ [ -LRB- cord-001257-t21l6i3f 348 19 17 17 CD cord-001257-t21l6i3f 348 20 ] ] -RRB- cord-001257-t21l6i3f 348 21 , , , cord-001257-t21l6i3f 348 22 with with IN cord-001257-t21l6i3f 348 23 modifications modification NNS cord-001257-t21l6i3f 348 24 : : : cord-001257-t21l6i3f 348 25 the the DT cord-001257-t21l6i3f 348 26 binding bind VBG cord-001257-t21l6i3f 348 27 assay assay NN cord-001257-t21l6i3f 348 28 was be VBD cord-001257-t21l6i3f 348 29 done do VBN cord-001257-t21l6i3f 348 30 in in IN cord-001257-t21l6i3f 348 31 the the DT cord-001257-t21l6i3f 348 32 presence presence NN cord-001257-t21l6i3f 348 33 of of IN cord-001257-t21l6i3f 348 34 20 20 CD cord-001257-t21l6i3f 348 35 mM mm CD cord-001257-t21l6i3f 348 36 HEPES HEPES NNP cord-001257-t21l6i3f 348 37 ( ( -LRB- cord-001257-t21l6i3f 348 38 pH pH NNP cord-001257-t21l6i3f 348 39 7.4 7.4 CD cord-001257-t21l6i3f 348 40 ) ) -RRB- cord-001257-t21l6i3f 348 41 , , , cord-001257-t21l6i3f 348 42 50 50 CD cord-001257-t21l6i3f 348 43 mM mm CD cord-001257-t21l6i3f 348 44 NaCl nacl NN cord-001257-t21l6i3f 348 45 , , , cord-001257-t21l6i3f 348 46 10 10 CD cord-001257-t21l6i3f 348 47 mM mm CD cord-001257-t21l6i3f 348 48 MgCl MgCl NNP cord-001257-t21l6i3f 348 49 2 2 CD cord-001257-t21l6i3f 348 50 , , , cord-001257-t21l6i3f 348 51 1 1 CD cord-001257-t21l6i3f 348 52 mM mm CD cord-001257-t21l6i3f 348 53 DTT dtt NN cord-001257-t21l6i3f 348 54 , , , cord-001257-t21l6i3f 349 1 1 1 LS cord-001257-t21l6i3f 349 2 mM mm CD cord-001257-t21l6i3f 349 3 EDTA EDTA NNP cord-001257-t21l6i3f 349 4 5 5 CD cord-001257-t21l6i3f 349 5 % % NN cord-001257-t21l6i3f 349 6 glycerol glycerol NN cord-001257-t21l6i3f 349 7 , , , cord-001257-t21l6i3f 349 8 6 6 CD cord-001257-t21l6i3f 349 9 U u NN cord-001257-t21l6i3f 349 10 of of IN cord-001257-t21l6i3f 349 11 RNasin RNasin NNP cord-001257-t21l6i3f 349 12 and and CC cord-001257-t21l6i3f 349 13 0.1 0.1 CD cord-001257-t21l6i3f 350 1 mg mg NNP cord-001257-t21l6i3f 350 2 tRNA tRNA NNP cord-001257-t21l6i3f 350 3 in in IN cord-001257-t21l6i3f 350 4 a a DT cord-001257-t21l6i3f 350 5 10 10 CD cord-001257-t21l6i3f 350 6 ml ml NN cord-001257-t21l6i3f 350 7 reaction reaction NN cord-001257-t21l6i3f 350 8 volume volume NN cord-001257-t21l6i3f 350 9 . . . cord-001257-t21l6i3f 351 1 Approximately approximately RB cord-001257-t21l6i3f 351 2 0.1 0.1 CD cord-001257-t21l6i3f 351 3 pmol pmol NN cord-001257-t21l6i3f 351 4 of of IN cord-001257-t21l6i3f 351 5 32 32 CD cord-001257-t21l6i3f 351 6 Plabeled Plabeled NNP cord-001257-t21l6i3f 351 7 RNA RNA NNP cord-001257-t21l6i3f 351 8 probes probe NNS cord-001257-t21l6i3f 351 9 , , , cord-001257-t21l6i3f 351 10 0.6 0.6 CD cord-001257-t21l6i3f 351 11 mg mg NN cord-001257-t21l6i3f 351 12 of of IN cord-001257-t21l6i3f 351 13 purified purify VBN cord-001257-t21l6i3f 351 14 recombinant recombinant JJ cord-001257-t21l6i3f 351 15 proteins protein NNS cord-001257-t21l6i3f 351 16 and and CC cord-001257-t21l6i3f 351 17 0.15 0.15 CD cord-001257-t21l6i3f 351 18 or or CC cord-001257-t21l6i3f 351 19 0.3 0.3 CD cord-001257-t21l6i3f 351 20 mg mg NN cord-001257-t21l6i3f 351 21 of of IN cord-001257-t21l6i3f 351 22 unlabeled unlabeled JJ cord-001257-t21l6i3f 351 23 RNA RNA NNP cord-001257-t21l6i3f 351 24 were be VBD cord-001257-t21l6i3f 351 25 used use VBN cord-001257-t21l6i3f 351 26 in in IN cord-001257-t21l6i3f 351 27 template template NN cord-001257-t21l6i3f 351 28 competition competition NN cord-001257-t21l6i3f 351 29 assay assay NN cord-001257-t21l6i3f 351 30 . . . cord-001257-t21l6i3f 352 1 For for IN cord-001257-t21l6i3f 352 2 the the DT cord-001257-t21l6i3f 352 3 assay assay NN cord-001257-t21l6i3f 352 4 , , , cord-001257-t21l6i3f 352 5 we -PRON- PRP cord-001257-t21l6i3f 352 6 used use VBD cord-001257-t21l6i3f 352 7 0.02 0.02 CD cord-001257-t21l6i3f 352 8 mg mg NNP cord-001257-t21l6i3f 352 9 MBP MBP NNP cord-001257-t21l6i3f 352 10 - - HYPH cord-001257-t21l6i3f 352 11 p33C p33C NNP cord-001257-t21l6i3f 352 12 , , , cord-001257-t21l6i3f 353 1 0.1 0.1 CD cord-001257-t21l6i3f 353 2 pmol pmol NN cord-001257-t21l6i3f 353 3 of of IN cord-001257-t21l6i3f 353 4 32 32 CD cord-001257-t21l6i3f 353 5 P p NN cord-001257-t21l6i3f 353 6 - - HYPH cord-001257-t21l6i3f 353 7 labeled label VBN cord-001257-t21l6i3f 353 8 SLR2 SLR2 NNP cord-001257-t21l6i3f 353 9 RNA RNA NNP cord-001257-t21l6i3f 353 10 ( ( -LRB- cord-001257-t21l6i3f 353 11 the the DT cord-001257-t21l6i3f 353 12 stem stem NN cord-001257-t21l6i3f 353 13 - - HYPH cord-001257-t21l6i3f 353 14 loop loop NN cord-001257-t21l6i3f 353 15 sequence sequence NN cord-001257-t21l6i3f 353 16 from from IN cord-001257-t21l6i3f 353 17 RII RII NNP cord-001257-t21l6i3f 353 18 ) ) -RRB- cord-001257-t21l6i3f 353 19 [ [ -LRB- cord-001257-t21l6i3f 353 20 17 17 CD cord-001257-t21l6i3f 353 21 ] ] -RRB- cord-001257-t21l6i3f 353 22 and and CC cord-001257-t21l6i3f 353 23 MBP MBP NNP cord-001257-t21l6i3f 353 24 - - HYPH cord-001257-t21l6i3f 353 25 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 353 26 ( ( -LRB- cord-001257-t21l6i3f 353 27 or or CC cord-001257-t21l6i3f 353 28 MBP MBP NNP cord-001257-t21l6i3f 353 29 - - HYPH cord-001257-t21l6i3f 353 30 AtRH5 AtRH5 NNP cord-001257-t21l6i3f 353 31 ) ) -RRB- cord-001257-t21l6i3f 353 32 , , , cord-001257-t21l6i3f 353 33 in in IN cord-001257-t21l6i3f 353 34 0.02 0.02 CD cord-001257-t21l6i3f 353 35 , , , cord-001257-t21l6i3f 353 36 0.06 0.06 CD cord-001257-t21l6i3f 353 37 , , , cord-001257-t21l6i3f 353 38 0.2 0.2 CD cord-001257-t21l6i3f 353 39 or or CC cord-001257-t21l6i3f 353 40 0.6 0.6 CD cord-001257-t21l6i3f 353 41 mg mg NN cord-001257-t21l6i3f 353 42 amounts amount NNS cord-001257-t21l6i3f 353 43 . . . cord-001257-t21l6i3f 354 1 Strand strand NN cord-001257-t21l6i3f 354 2 separation separation NN cord-001257-t21l6i3f 354 3 assay assay NN cord-001257-t21l6i3f 354 4 was be VBD cord-001257-t21l6i3f 354 5 performed perform VBN cord-001257-t21l6i3f 354 6 as as IN cord-001257-t21l6i3f 354 7 published publish VBN cord-001257-t21l6i3f 354 8 [ [ -LRB- cord-001257-t21l6i3f 354 9 58 58 CD cord-001257-t21l6i3f 354 10 ] ] -RRB- cord-001257-t21l6i3f 354 11 . . . cord-001257-t21l6i3f 355 1 Briefly briefly RB cord-001257-t21l6i3f 355 2 , , , cord-001257-t21l6i3f 355 3 about about RB cord-001257-t21l6i3f 355 4 2 2 CD cord-001257-t21l6i3f 355 5 pmol pmol NN cord-001257-t21l6i3f 355 6 of of IN cord-001257-t21l6i3f 355 7 32 32 CD cord-001257-t21l6i3f 355 8 P p NN cord-001257-t21l6i3f 355 9 - - HYPH cord-001257-t21l6i3f 355 10 labeled label VBN cord-001257-t21l6i3f 355 11 RIII RIII NNP cord-001257-t21l6i3f 355 12 ( ( -LRB- cord-001257-t21l6i3f 355 13 2 2 CD cord-001257-t21l6i3f 355 14 ) ) -RRB- cord-001257-t21l6i3f 355 15 or or CC cord-001257-t21l6i3f 355 16 RI RI NNP cord-001257-t21l6i3f 355 17 / / SYM cord-001257-t21l6i3f 355 18 II II NNP cord-001257-t21l6i3f 355 19 / / SYM cord-001257-t21l6i3f 355 20 III(2 III(2 NNP cord-001257-t21l6i3f 355 21 ) ) -RRB- cord-001257-t21l6i3f 355 22 were be VBD cord-001257-t21l6i3f 355 23 annealed anneal VBN cord-001257-t21l6i3f 355 24 to to IN cord-001257-t21l6i3f 355 25 unlabeled unlabeled JJ cord-001257-t21l6i3f 355 26 DI-72 DI-72 NNP cord-001257-t21l6i3f 355 27 ( ( -LRB- cord-001257-t21l6i3f 355 28 + + CC cord-001257-t21l6i3f 355 29 ) ) -RRB- cord-001257-t21l6i3f 355 30 RNA RNA NNP cord-001257-t21l6i3f 355 31 in in IN cord-001257-t21l6i3f 355 32 STE STE NNP cord-001257-t21l6i3f 355 33 buffer buffer NN cord-001257-t21l6i3f 355 34 by by IN cord-001257-t21l6i3f 355 35 slowly slowly RB cord-001257-t21l6i3f 355 36 cooling cool VBG cord-001257-t21l6i3f 355 37 down down RP cord-001257-t21l6i3f 355 38 the the DT cord-001257-t21l6i3f 355 39 samples sample NNS cord-001257-t21l6i3f 355 40 ( ( -LRB- cord-001257-t21l6i3f 355 41 in in IN cord-001257-t21l6i3f 355 42 20 20 CD cord-001257-t21l6i3f 355 43 ml ml NNS cord-001257-t21l6i3f 355 44 ) ) -RRB- cord-001257-t21l6i3f 355 45 from from IN cord-001257-t21l6i3f 355 46 94uC 94uc CD cord-001257-t21l6i3f 355 47 to to IN cord-001257-t21l6i3f 355 48 25uC 25uc CD cord-001257-t21l6i3f 355 49 in in IN cord-001257-t21l6i3f 355 50 30 30 CD cord-001257-t21l6i3f 355 51 min min NN cord-001257-t21l6i3f 355 52 . . . cord-001257-t21l6i3f 356 1 0.6 0.6 CD cord-001257-t21l6i3f 356 2 mg mg NN cord-001257-t21l6i3f 356 3 of of IN cord-001257-t21l6i3f 356 4 purified purify VBN cord-001257-t21l6i3f 356 5 recombinant recombinant JJ cord-001257-t21l6i3f 356 6 helicase helicase NN cord-001257-t21l6i3f 356 7 proteins protein NNS cord-001257-t21l6i3f 356 8 ( ( -LRB- cord-001257-t21l6i3f 356 9 in in IN cord-001257-t21l6i3f 356 10 MBP MBP NNP cord-001257-t21l6i3f 356 11 elution elution NN cord-001257-t21l6i3f 356 12 buffer buffer NN cord-001257-t21l6i3f 356 13 ) ) -RRB- cord-001257-t21l6i3f 356 14 or or CC cord-001257-t21l6i3f 356 15 MBP MBP NNP cord-001257-t21l6i3f 356 16 as as IN cord-001257-t21l6i3f 356 17 a a DT cord-001257-t21l6i3f 356 18 negative negative JJ cord-001257-t21l6i3f 356 19 controls control NNS cord-001257-t21l6i3f 356 20 were be VBD cord-001257-t21l6i3f 356 21 added add VBN cord-001257-t21l6i3f 356 22 separately separately RB cord-001257-t21l6i3f 356 23 to to IN cord-001257-t21l6i3f 356 24 the the DT cord-001257-t21l6i3f 356 25 partial partial JJ cord-001257-t21l6i3f 356 26 dsRNA dsrna NN cord-001257-t21l6i3f 356 27 duplex duplex NN cord-001257-t21l6i3f 356 28 in in IN cord-001257-t21l6i3f 356 29 the the DT cord-001257-t21l6i3f 356 30 RdRp RdRp NNP cord-001257-t21l6i3f 356 31 buffer buffer NN cord-001257-t21l6i3f 356 32 . . . cord-001257-t21l6i3f 357 1 2 2 LS cord-001257-t21l6i3f 357 2 mM mm CD cord-001257-t21l6i3f 357 3 of of IN cord-001257-t21l6i3f 357 4 ATP ATP NNP cord-001257-t21l6i3f 357 5 was be VBD cord-001257-t21l6i3f 357 6 added add VBN cord-001257-t21l6i3f 357 7 to to IN cord-001257-t21l6i3f 357 8 the the DT cord-001257-t21l6i3f 357 9 reaction reaction NN cord-001257-t21l6i3f 357 10 . . . cord-001257-t21l6i3f 358 1 Reaction reaction NN cord-001257-t21l6i3f 358 2 mixtures mixture NNS cord-001257-t21l6i3f 358 3 were be VBD cord-001257-t21l6i3f 358 4 incubated incubate VBN cord-001257-t21l6i3f 358 5 for for IN cord-001257-t21l6i3f 358 6 15 15 CD cord-001257-t21l6i3f 358 7 min min NN cord-001257-t21l6i3f 358 8 at at IN cord-001257-t21l6i3f 358 9 room room NN cord-001257-t21l6i3f 358 10 temperature temperature NN cord-001257-t21l6i3f 358 11 and and CC cord-001257-t21l6i3f 358 12 loaded load VBN cord-001257-t21l6i3f 358 13 onto onto IN cord-001257-t21l6i3f 358 14 5 5 CD cord-001257-t21l6i3f 358 15 % % NN cord-001257-t21l6i3f 358 16 nondenaturing nondenature VBG cord-001257-t21l6i3f 358 17 polyacrylamide polyacrylamide NN cord-001257-t21l6i3f 358 18 gel gel NN cord-001257-t21l6i3f 358 19 as as IN cord-001257-t21l6i3f 358 20 described describe VBN cord-001257-t21l6i3f 358 21 previously previously RB cord-001257-t21l6i3f 358 22 [ [ -LRB- cord-001257-t21l6i3f 358 23 58 58 CD cord-001257-t21l6i3f 358 24 ] ] -RRB- cord-001257-t21l6i3f 358 25 . . . cord-001257-t21l6i3f 359 1 Some some DT cord-001257-t21l6i3f 359 2 samples sample NNS cord-001257-t21l6i3f 359 3 were be VBD cord-001257-t21l6i3f 359 4 treated treat VBN cord-001257-t21l6i3f 359 5 with with IN cord-001257-t21l6i3f 359 6 proteinase proteinase NN cord-001257-t21l6i3f 359 7 K k NN cord-001257-t21l6i3f 359 8 after after IN cord-001257-t21l6i3f 359 9 the the DT cord-001257-t21l6i3f 359 10 assay assay NN cord-001257-t21l6i3f 359 11 . . . cord-001257-t21l6i3f 360 1 The the DT cord-001257-t21l6i3f 360 2 incubation incubation NN cord-001257-t21l6i3f 360 3 with with IN cord-001257-t21l6i3f 360 4 proteinase proteinase NN cord-001257-t21l6i3f 360 5 K K NNP cord-001257-t21l6i3f 360 6 lasted last VBD cord-001257-t21l6i3f 360 7 for for IN cord-001257-t21l6i3f 360 8 10 10 CD cord-001257-t21l6i3f 360 9 min min NN cord-001257-t21l6i3f 360 10 at at IN cord-001257-t21l6i3f 360 11 37uC 37uc CD cord-001257-t21l6i3f 360 12 using use VBG cord-001257-t21l6i3f 360 13 0.5 0.5 CD cord-001257-t21l6i3f 360 14 ml ml NNS cord-001257-t21l6i3f 360 15 of of IN cord-001257-t21l6i3f 360 16 proteinase proteinase NN cord-001257-t21l6i3f 360 17 K K NNP cord-001257-t21l6i3f 360 18 from from IN cord-001257-t21l6i3f 360 19 stock stock NN cord-001257-t21l6i3f 360 20 of of IN cord-001257-t21l6i3f 360 21 20 20 CD cord-001257-t21l6i3f 360 22 mg mg NNP cord-001257-t21l6i3f 360 23 / / SYM cord-001257-t21l6i3f 360 24 ml ml NN cord-001257-t21l6i3f 360 25 ( ( -LRB- cord-001257-t21l6i3f 360 26 dissolved dissolve VBN cord-001257-t21l6i3f 360 27 in in IN cord-001257-t21l6i3f 360 28 50 50 CD cord-001257-t21l6i3f 360 29 mM mm CD cord-001257-t21l6i3f 360 30 Tris Tris NNP cord-001257-t21l6i3f 360 31 - - HYPH cord-001257-t21l6i3f 360 32 HCl HCl NNP cord-001257-t21l6i3f 360 33 pH pH NNP cord-001257-t21l6i3f 360 34 8.0 8.0 CD cord-001257-t21l6i3f 360 35 , , , cord-001257-t21l6i3f 360 36 supplemented supplement VBN cord-001257-t21l6i3f 360 37 with with IN cord-001257-t21l6i3f 360 38 1.5 1.5 CD cord-001257-t21l6i3f 360 39 mM mm CD cord-001257-t21l6i3f 360 40 CaCl CaCl NNP cord-001257-t21l6i3f 360 41 2 2 CD cord-001257-t21l6i3f 360 42 ) ) -RRB- cord-001257-t21l6i3f 360 43 , , , cord-001257-t21l6i3f 360 44 followed follow VBN cord-001257-t21l6i3f 360 45 by by IN cord-001257-t21l6i3f 360 46 loading load VBG cord-001257-t21l6i3f 360 47 onto onto IN cord-001257-t21l6i3f 360 48 5 5 CD cord-001257-t21l6i3f 360 49 % % NN cord-001257-t21l6i3f 360 50 nondenaturing nondenature VBG cord-001257-t21l6i3f 360 51 polyacrylamide polyacrylamide NN cord-001257-t21l6i3f 360 52 gel gel NN cord-001257-t21l6i3f 360 53 . . . cord-001257-t21l6i3f 361 1 The the DT cord-001257-t21l6i3f 361 2 UV uv NN cord-001257-t21l6i3f 361 3 - - HYPH cord-001257-t21l6i3f 361 4 cross cross NN cord-001257-t21l6i3f 361 5 - - JJ cord-001257-t21l6i3f 361 6 linking linking JJ cord-001257-t21l6i3f 361 7 assay assay NN cord-001257-t21l6i3f 361 8 was be VBD cord-001257-t21l6i3f 361 9 performed perform VBN cord-001257-t21l6i3f 361 10 as as IN cord-001257-t21l6i3f 361 11 described describe VBN cord-001257-t21l6i3f 361 12 [ [ -LRB- cord-001257-t21l6i3f 361 13 98 98 CD cord-001257-t21l6i3f 361 14 ] ] -RRB- cord-001257-t21l6i3f 361 15 . . . cord-001257-t21l6i3f 362 1 The the DT cord-001257-t21l6i3f 362 2 10 10 CD cord-001257-t21l6i3f 362 3 ml ml NN cord-001257-t21l6i3f 362 4 reaction reaction NN cord-001257-t21l6i3f 362 5 mixture mixture NN cord-001257-t21l6i3f 362 6 contained contain VBD cord-001257-t21l6i3f 362 7 1 1 CD cord-001257-t21l6i3f 362 8 mg mg NN cord-001257-t21l6i3f 362 9 purified purify VBN cord-001257-t21l6i3f 362 10 MBP MBP NNP cord-001257-t21l6i3f 362 11 - - HYPH cord-001257-t21l6i3f 362 12 tagged tag VBN cord-001257-t21l6i3f 362 13 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 362 14 or or CC cord-001257-t21l6i3f 362 15 AtRH5 atrh5 NN cord-001257-t21l6i3f 362 16 proteins protein NNS cord-001257-t21l6i3f 362 17 , , , cord-001257-t21l6i3f 362 18 respectively respectively RB cord-001257-t21l6i3f 362 19 , , , cord-001257-t21l6i3f 362 20 0.5 0.5 CD cord-001257-t21l6i3f 362 21 nM nm CD cord-001257-t21l6i3f 362 22 32 32 CD cord-001257-t21l6i3f 362 23 P p NN cord-001257-t21l6i3f 362 24 - - HYPH cord-001257-t21l6i3f 362 25 UTP UTP NNP cord-001257-t21l6i3f 362 26 - - HYPH cord-001257-t21l6i3f 362 27 labeled label VBN cord-001257-t21l6i3f 362 28 RNA RNA NNP cord-001257-t21l6i3f 362 29 probe probe NN cord-001257-t21l6i3f 362 30 , , , cord-001257-t21l6i3f 363 1 10 10 CD cord-001257-t21l6i3f 363 2 mM mm CD cord-001257-t21l6i3f 363 3 HEPES HEPES NNP cord-001257-t21l6i3f 363 4 , , , cord-001257-t21l6i3f 363 5 pH pH NNP cord-001257-t21l6i3f 363 6 7.9 7.9 CD cord-001257-t21l6i3f 363 7 , , , cord-001257-t21l6i3f 363 8 100 100 CD cord-001257-t21l6i3f 363 9 mM mm CD cord-001257-t21l6i3f 363 10 KCl KCl NNS cord-001257-t21l6i3f 363 11 , , , cord-001257-t21l6i3f 363 12 1 1 CD cord-001257-t21l6i3f 363 13 mM mm CD cord-001257-t21l6i3f 363 14 MgCl MgCl NNP cord-001257-t21l6i3f 363 15 2 2 CD cord-001257-t21l6i3f 363 16 , , , cord-001257-t21l6i3f 363 17 10 10 CD cord-001257-t21l6i3f 363 18 % % NN cord-001257-t21l6i3f 363 19 glycerol glycerol NN cord-001257-t21l6i3f 363 20 , , , cord-001257-t21l6i3f 363 21 and and CC cord-001257-t21l6i3f 363 22 1 1 CD cord-001257-t21l6i3f 363 23 mg mg NNP cord-001257-t21l6i3f 363 24 tRNA tRNA NNP cord-001257-t21l6i3f 363 25 . . . cord-001257-t21l6i3f 364 1 Unlabeled unlabeled JJ cord-001257-t21l6i3f 364 2 RNA RNA NNP cord-001257-t21l6i3f 364 3 transcripts transcript NNS cord-001257-t21l6i3f 364 4 of of IN cord-001257-t21l6i3f 364 5 RIII(2 RIII(2 NNP cord-001257-t21l6i3f 364 6 ) ) -RRB- cord-001257-t21l6i3f 364 7 or or CC cord-001257-t21l6i3f 364 8 ' ' `` cord-001257-t21l6i3f 364 9 ' ' '' cord-001257-t21l6i3f 364 10 + + CC cord-001257-t21l6i3f 364 11 bridge bridge NN cord-001257-t21l6i3f 364 12 ' ' '' cord-001257-t21l6i3f 364 13 ' ' '' cord-001257-t21l6i3f 364 14 and and CC cord-001257-t21l6i3f 364 15 ' ' '' cord-001257-t21l6i3f 364 16 ' ' `` cord-001257-t21l6i3f 364 17 Dbridge Dbridge NNP cord-001257-t21l6i3f 364 18 ' ' '' cord-001257-t21l6i3f 364 19 ' ' '' cord-001257-t21l6i3f 364 20 constructs construct NNS cord-001257-t21l6i3f 364 21 ( ( -LRB- cord-001257-t21l6i3f 364 22 all all DT cord-001257-t21l6i3f 364 23 RNAs rna NNS cord-001257-t21l6i3f 364 24 were be VBD cord-001257-t21l6i3f 364 25 comparable comparable JJ cord-001257-t21l6i3f 364 26 in in IN cord-001257-t21l6i3f 364 27 length length NN cord-001257-t21l6i3f 364 28 ) ) -RRB- cord-001257-t21l6i3f 364 29 were be VBD cord-001257-t21l6i3f 364 30 used use VBN cord-001257-t21l6i3f 364 31 as as IN cord-001257-t21l6i3f 364 32 competitors competitor NNS cord-001257-t21l6i3f 364 33 in in IN cord-001257-t21l6i3f 364 34 0.1 0.1 CD cord-001257-t21l6i3f 364 35 to to TO cord-001257-t21l6i3f 364 36 0.3 0.3 CD cord-001257-t21l6i3f 364 37 mg mg NN cord-001257-t21l6i3f 364 38 amounts amount NNS cord-001257-t21l6i3f 364 39 in in IN cord-001257-t21l6i3f 364 40 the the DT cord-001257-t21l6i3f 364 41 competition competition NN cord-001257-t21l6i3f 364 42 assay assay NN cord-001257-t21l6i3f 364 43 . . . cord-001257-t21l6i3f 365 1 After after IN cord-001257-t21l6i3f 365 2 the the DT cord-001257-t21l6i3f 365 3 formation formation NN cord-001257-t21l6i3f 365 4 of of IN cord-001257-t21l6i3f 365 5 RNAprotein RNAprotein NNP cord-001257-t21l6i3f 365 6 complexes complex NNS cord-001257-t21l6i3f 365 7 during during IN cord-001257-t21l6i3f 365 8 incubation incubation NN cord-001257-t21l6i3f 365 9 of of IN cord-001257-t21l6i3f 365 10 the the DT cord-001257-t21l6i3f 365 11 reaction reaction NN cord-001257-t21l6i3f 365 12 mixtures mixture NNS cord-001257-t21l6i3f 365 13 at at IN cord-001257-t21l6i3f 365 14 room room NN cord-001257-t21l6i3f 365 15 temperature temperature NN cord-001257-t21l6i3f 365 16 for for IN cord-001257-t21l6i3f 365 17 , , , cord-001257-t21l6i3f 365 18 15 15 CD cord-001257-t21l6i3f 365 19 min min NN cord-001257-t21l6i3f 365 20 , , , cord-001257-t21l6i3f 365 21 we -PRON- PRP cord-001257-t21l6i3f 365 22 transferred transfer VBD cord-001257-t21l6i3f 365 23 the the DT cord-001257-t21l6i3f 365 24 reaction reaction NN cord-001257-t21l6i3f 365 25 mixtures mixture NNS cord-001257-t21l6i3f 365 26 to to IN cord-001257-t21l6i3f 365 27 a a DT cord-001257-t21l6i3f 365 28 96-well 96-well CD cord-001257-t21l6i3f 365 29 plate plate NN cord-001257-t21l6i3f 365 30 on on IN cord-001257-t21l6i3f 365 31 ice ice NN cord-001257-t21l6i3f 365 32 . . . cord-001257-t21l6i3f 366 1 To to TO cord-001257-t21l6i3f 366 2 cross cross VB cord-001257-t21l6i3f 366 3 - - JJ cord-001257-t21l6i3f 366 4 link link VB cord-001257-t21l6i3f 366 5 the the DT cord-001257-t21l6i3f 366 6 RNA rna NN cord-001257-t21l6i3f 366 7 and and CC cord-001257-t21l6i3f 366 8 protein protein NN cord-001257-t21l6i3f 366 9 , , , cord-001257-t21l6i3f 366 10 we -PRON- PRP cord-001257-t21l6i3f 366 11 irradiated irradiate VBD cord-001257-t21l6i3f 366 12 the the DT cord-001257-t21l6i3f 366 13 reaction reaction NN cord-001257-t21l6i3f 366 14 mixtures mixture NNS cord-001257-t21l6i3f 366 15 on on IN cord-001257-t21l6i3f 366 16 ice ice NN cord-001257-t21l6i3f 366 17 at at IN cord-001257-t21l6i3f 366 18 254 254 CD cord-001257-t21l6i3f 366 19 nm nm CD cord-001257-t21l6i3f 366 20 wave wave NN cord-001257-t21l6i3f 366 21 - - HYPH cord-001257-t21l6i3f 366 22 length length NN cord-001257-t21l6i3f 366 23 for for IN cord-001257-t21l6i3f 366 24 10 10 CD cord-001257-t21l6i3f 366 25 min min NN cord-001257-t21l6i3f 366 26 using use VBG cord-001257-t21l6i3f 366 27 an an DT cord-001257-t21l6i3f 366 28 UV uv NN cord-001257-t21l6i3f 366 29 Stratalinker Stratalinker NNP cord-001257-t21l6i3f 366 30 1800 1800 CD cord-001257-t21l6i3f 366 31 ( ( -LRB- cord-001257-t21l6i3f 366 32 Stratagene Stratagene NNP cord-001257-t21l6i3f 366 33 ) ) -RRB- cord-001257-t21l6i3f 366 34 . . . cord-001257-t21l6i3f 367 1 Then then RB cord-001257-t21l6i3f 367 2 , , , cord-001257-t21l6i3f 367 3 we -PRON- PRP cord-001257-t21l6i3f 367 4 digested digest VBD cord-001257-t21l6i3f 367 5 the the DT cord-001257-t21l6i3f 367 6 unprotected unprotected JJ cord-001257-t21l6i3f 367 7 RNAs rna NNS cord-001257-t21l6i3f 367 8 by by IN cord-001257-t21l6i3f 367 9 1 1 CD cord-001257-t21l6i3f 367 10 mg mg NNP cord-001257-t21l6i3f 367 11 / / SYM cord-001257-t21l6i3f 367 12 ml ml XX cord-001257-t21l6i3f 367 13 RNase RNase NNP cord-001257-t21l6i3f 367 14 A a NN cord-001257-t21l6i3f 367 15 for for IN cord-001257-t21l6i3f 367 16 15 15 CD cord-001257-t21l6i3f 367 17 min min NN cord-001257-t21l6i3f 367 18 at at IN cord-001257-t21l6i3f 367 19 37uC. 37uc. CD cord-001257-t21l6i3f 367 20 Samples sample NNS cord-001257-t21l6i3f 367 21 were be VBD cord-001257-t21l6i3f 367 22 mixed mix VBN cord-001257-t21l6i3f 367 23 and and CC cord-001257-t21l6i3f 367 24 boiled boil VBN cord-001257-t21l6i3f 367 25 for for IN cord-001257-t21l6i3f 367 26 10 10 CD cord-001257-t21l6i3f 367 27 min min NN cord-001257-t21l6i3f 367 28 in in IN cord-001257-t21l6i3f 367 29 16 16 CD cord-001257-t21l6i3f 367 30 SDS SDS NNP cord-001257-t21l6i3f 367 31 loading loading NN cord-001257-t21l6i3f 367 32 dye dye NN cord-001257-t21l6i3f 367 33 . . . cord-001257-t21l6i3f 368 1 Analysis analysis NN cord-001257-t21l6i3f 368 2 was be VBD cord-001257-t21l6i3f 368 3 performed perform VBN cord-001257-t21l6i3f 368 4 using use VBG cord-001257-t21l6i3f 368 5 SDS SDS NNP cord-001257-t21l6i3f 368 6 - - HYPH cord-001257-t21l6i3f 368 7 PAGE PAGE NNP cord-001257-t21l6i3f 368 8 and and CC cord-001257-t21l6i3f 368 9 phosphorimaging phosphorimage VBG cord-001257-t21l6i3f 368 10 [ [ -LRB- cord-001257-t21l6i3f 368 11 98 98 CD cord-001257-t21l6i3f 368 12 ] ] -RRB- cord-001257-t21l6i3f 368 13 . . . cord-001257-t21l6i3f 369 1 Yeast yeast NN cord-001257-t21l6i3f 369 2 strain strain NN cord-001257-t21l6i3f 369 3 Ddbp3 ddbp3 NN cord-001257-t21l6i3f 369 4 was be VBD cord-001257-t21l6i3f 369 5 transformed transform VBN cord-001257-t21l6i3f 369 6 with with IN cord-001257-t21l6i3f 369 7 plasmids plasmid NNS cord-001257-t21l6i3f 369 8 pGBK pgbk JJ cord-001257-t21l6i3f 369 9 - - HYPH cord-001257-t21l6i3f 369 10 HIS HIS NNP cord-001257-t21l6i3f 369 11 - - HYPH cord-001257-t21l6i3f 369 12 Cup Cup NNP cord-001257-t21l6i3f 369 13 - - HYPH cord-001257-t21l6i3f 369 14 Flag33 Flag33 NNP cord-001257-t21l6i3f 369 15 / / SYM cord-001257-t21l6i3f 369 16 Gal Gal NNP cord-001257-t21l6i3f 369 17 - - HYPH cord-001257-t21l6i3f 369 18 DI-72 DI-72 NNP cord-001257-t21l6i3f 369 19 , , , cord-001257-t21l6i3f 369 20 pGAD pGAD NNP cord-001257-t21l6i3f 369 21 - - HYPH cord-001257-t21l6i3f 369 22 Cup Cup NNP cord-001257-t21l6i3f 369 23 - - HYPH cord-001257-t21l6i3f 369 24 Flagp92 Flagp92 NNP cord-001257-t21l6i3f 369 25 and and CC cord-001257-t21l6i3f 369 26 pYC pYC NNP cord-001257-t21l6i3f 369 27 - - HYPH cord-001257-t21l6i3f 369 28 Gal-66HisRH2 gal-66hisrh2 CD cord-001257-t21l6i3f 369 29 or or CC cord-001257-t21l6i3f 369 30 pYC pYC NNP cord-001257-t21l6i3f 369 31 - - HYPH cord-001257-t21l6i3f 369 32 Gal-66HisRH5 Gal-66HisRH5 NNS cord-001257-t21l6i3f 369 33 to to TO cord-001257-t21l6i3f 369 34 co co VB cord-001257-t21l6i3f 369 35 - - VB cord-001257-t21l6i3f 369 36 express express VB cord-001257-t21l6i3f 369 37 the the DT cord-001257-t21l6i3f 369 38 cellular cellular JJ cord-001257-t21l6i3f 369 39 helicases helicase NNS cord-001257-t21l6i3f 369 40 with with IN cord-001257-t21l6i3f 369 41 the the DT cord-001257-t21l6i3f 369 42 viral viral JJ cord-001257-t21l6i3f 369 43 replication replication NN cord-001257-t21l6i3f 369 44 proteins protein NNS cord-001257-t21l6i3f 369 45 in in IN cord-001257-t21l6i3f 369 46 yeast yeast NN cord-001257-t21l6i3f 369 47 cells cell NNS cord-001257-t21l6i3f 369 48 actively actively RB cord-001257-t21l6i3f 369 49 replicating replicate VBG cord-001257-t21l6i3f 369 50 the the DT cord-001257-t21l6i3f 369 51 TBSV TBSV NNP cord-001257-t21l6i3f 369 52 repRNA repRNA NNS cord-001257-t21l6i3f 369 53 . . . cord-001257-t21l6i3f 370 1 The the DT cord-001257-t21l6i3f 370 2 transformed transform VBN cord-001257-t21l6i3f 370 3 yeast yeast NN cord-001257-t21l6i3f 370 4 strains strain NNS cord-001257-t21l6i3f 370 5 were be VBD cord-001257-t21l6i3f 370 6 selected select VBN cord-001257-t21l6i3f 370 7 on on IN cord-001257-t21l6i3f 370 8 SC SC NNP cord-001257-t21l6i3f 370 9 - - HYPH cord-001257-t21l6i3f 370 10 ULH ULH NNP cord-001257-t21l6i3f 370 11 2 2 CD cord-001257-t21l6i3f 370 12 plates plate NNS cord-001257-t21l6i3f 370 13 and and CC cord-001257-t21l6i3f 370 14 then then RB cord-001257-t21l6i3f 370 15 pre pre VB cord-001257-t21l6i3f 370 16 - - JJ cord-001257-t21l6i3f 370 17 grown grown JJ cord-001257-t21l6i3f 370 18 overnight overnight RB cord-001257-t21l6i3f 370 19 at at IN cord-001257-t21l6i3f 370 20 29uC 29uc CD cord-001257-t21l6i3f 370 21 in in IN cord-001257-t21l6i3f 370 22 selective selective JJ cord-001257-t21l6i3f 370 23 media medium NNS cord-001257-t21l6i3f 370 24 containing contain VBG cord-001257-t21l6i3f 370 25 2 2 CD cord-001257-t21l6i3f 370 26 % % NN cord-001257-t21l6i3f 370 27 glucose glucose NN cord-001257-t21l6i3f 370 28 [ [ -LRB- cord-001257-t21l6i3f 370 29 49 49 CD cord-001257-t21l6i3f 370 30 ] ] -RRB- cord-001257-t21l6i3f 370 31 . . . cord-001257-t21l6i3f 371 1 After after IN cord-001257-t21l6i3f 371 2 that that DT cord-001257-t21l6i3f 371 3 yeast yeast NN cord-001257-t21l6i3f 371 4 strains strain NNS cord-001257-t21l6i3f 371 5 were be VBD cord-001257-t21l6i3f 371 6 pelleted pellete VBN cord-001257-t21l6i3f 371 7 by by IN cord-001257-t21l6i3f 371 8 centrifugation centrifugation NN cord-001257-t21l6i3f 371 9 at at IN cord-001257-t21l6i3f 371 10 2,000 2,000 CD cord-001257-t21l6i3f 371 11 rpm rpm NN cord-001257-t21l6i3f 371 12 for for IN cord-001257-t21l6i3f 371 13 3 3 CD cord-001257-t21l6i3f 371 14 min min NN cord-001257-t21l6i3f 371 15 , , , cord-001257-t21l6i3f 371 16 we -PRON- PRP cord-001257-t21l6i3f 371 17 washed wash VBD cord-001257-t21l6i3f 371 18 the the DT cord-001257-t21l6i3f 371 19 pellet pellet NN cord-001257-t21l6i3f 371 20 with with IN cord-001257-t21l6i3f 371 21 SC SC NNP cord-001257-t21l6i3f 371 22 - - HYPH cord-001257-t21l6i3f 371 23 ULH ULH NNP cord-001257-t21l6i3f 371 24 2 2 CD cord-001257-t21l6i3f 371 25 media medium NNS cord-001257-t21l6i3f 371 26 containing contain VBG cord-001257-t21l6i3f 371 27 2 2 CD cord-001257-t21l6i3f 371 28 % % NN cord-001257-t21l6i3f 371 29 galactose galactose NN cord-001257-t21l6i3f 371 30 and and CC cord-001257-t21l6i3f 371 31 50 50 CD cord-001257-t21l6i3f 371 32 mM mm NN cord-001257-t21l6i3f 371 33 CuSO CuSO NNP cord-001257-t21l6i3f 371 34 4 4 CD cord-001257-t21l6i3f 371 35 , , , cord-001257-t21l6i3f 371 36 yeast yeast NN cord-001257-t21l6i3f 371 37 were be VBD cord-001257-t21l6i3f 371 38 grown grow VBN cord-001257-t21l6i3f 371 39 for for IN cord-001257-t21l6i3f 371 40 36 36 CD cord-001257-t21l6i3f 371 41 hours hour NNS cord-001257-t21l6i3f 371 42 in in IN cord-001257-t21l6i3f 371 43 SC SC NNP cord-001257-t21l6i3f 371 44 - - HYPH cord-001257-t21l6i3f 371 45 ULH ULH NNP cord-001257-t21l6i3f 371 46 2 2 CD cord-001257-t21l6i3f 371 47 media medium NNS cord-001257-t21l6i3f 371 48 containing contain VBG cord-001257-t21l6i3f 371 49 2 2 CD cord-001257-t21l6i3f 371 50 % % NN cord-001257-t21l6i3f 371 51 galactose galactose NN cord-001257-t21l6i3f 371 52 at at IN cord-001257-t21l6i3f 371 53 23uC. 23uc. CD cord-001257-t21l6i3f 371 54 Pelleted Pelleted NNP cord-001257-t21l6i3f 371 55 yeasts yeast NNS cord-001257-t21l6i3f 371 56 ( ( -LRB- cord-001257-t21l6i3f 371 57 about about RB cord-001257-t21l6i3f 371 58 200 200 CD cord-001257-t21l6i3f 371 59 mg mg NNS cord-001257-t21l6i3f 371 60 ) ) -RRB- cord-001257-t21l6i3f 371 61 were be VBD cord-001257-t21l6i3f 371 62 used use VBN cord-001257-t21l6i3f 371 63 for for IN cord-001257-t21l6i3f 371 64 affinity affinity NN cord-001257-t21l6i3f 371 65 - - HYPH cord-001257-t21l6i3f 371 66 purification purification NN cord-001257-t21l6i3f 371 67 of of IN cord-001257-t21l6i3f 371 68 FLAG flag NN cord-001257-t21l6i3f 371 69 - - HYPH cord-001257-t21l6i3f 371 70 p33 p33 NNP cord-001257-t21l6i3f 371 71 and and CC cord-001257-t21l6i3f 371 72 FLAG flag NN cord-001257-t21l6i3f 371 73 - - HYPH cord-001257-t21l6i3f 371 74 p92 p92 NN cord-001257-t21l6i3f 371 75 with with IN cord-001257-t21l6i3f 371 76 anti anti JJ cord-001257-t21l6i3f 371 77 - - JJ cord-001257-t21l6i3f 371 78 FLAG flag JJ cord-001257-t21l6i3f 371 79 M2 m2 NN cord-001257-t21l6i3f 371 80 agarose agarose VBD cord-001257-t21l6i3f 371 81 as as IN cord-001257-t21l6i3f 371 82 published publish VBN cord-001257-t21l6i3f 371 83 previously previously RB cord-001257-t21l6i3f 371 84 [ [ -LRB- cord-001257-t21l6i3f 371 85 15 15 CD cord-001257-t21l6i3f 371 86 , , , cord-001257-t21l6i3f 371 87 49 49 CD cord-001257-t21l6i3f 371 88 ] ] -RRB- cord-001257-t21l6i3f 371 89 . . . cord-001257-t21l6i3f 372 1 FLAG flag NN cord-001257-t21l6i3f 372 2 - - HYPH cord-001257-t21l6i3f 372 3 p33 p33 NNP cord-001257-t21l6i3f 372 4 was be VBD cord-001257-t21l6i3f 372 5 detected detect VBN cord-001257-t21l6i3f 372 6 with with IN cord-001257-t21l6i3f 372 7 anti anti JJ cord-001257-t21l6i3f 372 8 - - JJ cord-001257-t21l6i3f 372 9 Flag flag JJ cord-001257-t21l6i3f 372 10 antibody antibody NN cord-001257-t21l6i3f 372 11 ( ( -LRB- cord-001257-t21l6i3f 372 12 1/10,000 1/10,000 CD cord-001257-t21l6i3f 372 13 dilution dilution NN cord-001257-t21l6i3f 372 14 ) ) -RRB- cord-001257-t21l6i3f 372 15 and and CC cord-001257-t21l6i3f 372 16 AP AP NNP cord-001257-t21l6i3f 372 17 - - HYPH cord-001257-t21l6i3f 372 18 conjugated conjugate VBN cord-001257-t21l6i3f 372 19 anti anti JJ cord-001257-t21l6i3f 372 20 - - JJ cord-001257-t21l6i3f 372 21 mouse mouse JJ cord-001257-t21l6i3f 372 22 antibody antibody NN cord-001257-t21l6i3f 372 23 ( ( -LRB- cord-001257-t21l6i3f 372 24 1/10,000 1/10,000 CD cord-001257-t21l6i3f 372 25 ) ) -RRB- cord-001257-t21l6i3f 372 26 . . . cord-001257-t21l6i3f 373 1 His -PRON- PRP$ cord-001257-t21l6i3f 373 2 6 6 CD cord-001257-t21l6i3f 373 3 -AtRH2 -atrh2 CD cord-001257-t21l6i3f 373 4 or or CC cord-001257-t21l6i3f 373 5 His -PRON- PRP$ cord-001257-t21l6i3f 373 6 6 6 CD cord-001257-t21l6i3f 373 7 -AtRH5 -atrh5 NN cord-001257-t21l6i3f 373 8 proteins protein NNS cord-001257-t21l6i3f 373 9 were be VBD cord-001257-t21l6i3f 373 10 detected detect VBN cord-001257-t21l6i3f 373 11 with with IN cord-001257-t21l6i3f 373 12 anti anti JJ cord-001257-t21l6i3f 373 13 - - JJ cord-001257-t21l6i3f 373 14 His -PRON- PRP$ cord-001257-t21l6i3f 373 15 antibody antibody NN cord-001257-t21l6i3f 373 16 from from IN cord-001257-t21l6i3f 373 17 mouse mouse NN cord-001257-t21l6i3f 373 18 ( ( -LRB- cord-001257-t21l6i3f 373 19 1/10,000 1/10,000 CD cord-001257-t21l6i3f 373 20 dilution dilution NN cord-001257-t21l6i3f 373 21 ) ) -RRB- cord-001257-t21l6i3f 373 22 and and CC cord-001257-t21l6i3f 373 23 AP AP NNP cord-001257-t21l6i3f 373 24 - - HYPH cord-001257-t21l6i3f 373 25 conjugated conjugate VBN cord-001257-t21l6i3f 373 26 anti anti JJ cord-001257-t21l6i3f 373 27 - - JJ cord-001257-t21l6i3f 373 28 mouse mouse NN cord-001257-t21l6i3f 373 29 ( ( -LRB- cord-001257-t21l6i3f 373 30 1/10,000 1/10,000 CD cord-001257-t21l6i3f 373 31 ) ) -RRB- cord-001257-t21l6i3f 373 32 followed follow VBN cord-001257-t21l6i3f 373 33 by by IN cord-001257-t21l6i3f 373 34 NBT NBT NNP cord-001257-t21l6i3f 373 35 - - HYPH cord-001257-t21l6i3f 373 36 BCIP BCIP NNP cord-001257-t21l6i3f 373 37 detection detection NN cord-001257-t21l6i3f 373 38 [ [ -LRB- cord-001257-t21l6i3f 373 39 15 15 CD cord-001257-t21l6i3f 373 40 , , , cord-001257-t21l6i3f 373 41 49 49 CD cord-001257-t21l6i3f 373 42 ] ] -RRB- cord-001257-t21l6i3f 373 43 . . . cord-001257-t21l6i3f 374 1 The the DT cord-001257-t21l6i3f 374 2 split split NN cord-001257-t21l6i3f 374 3 - - HYPH cord-001257-t21l6i3f 374 4 ubiquitin ubiquitin NN cord-001257-t21l6i3f 374 5 assay assay NN cord-001257-t21l6i3f 374 6 was be VBD cord-001257-t21l6i3f 374 7 based base VBN cord-001257-t21l6i3f 374 8 on on IN cord-001257-t21l6i3f 374 9 the the DT cord-001257-t21l6i3f 374 10 Dualmembrane Dualmembrane NNP cord-001257-t21l6i3f 374 11 kit3 kit3 NNP cord-001257-t21l6i3f 374 12 ( ( -LRB- cord-001257-t21l6i3f 374 13 Dualsystems Dualsystems NNP cord-001257-t21l6i3f 374 14 ) ) -RRB- cord-001257-t21l6i3f 374 15 . . . cord-001257-t21l6i3f 375 1 pGAD pgad LS cord-001257-t21l6i3f 375 2 - - HYPH cord-001257-t21l6i3f 375 3 BT2-N BT2-N NNP cord-001257-t21l6i3f 375 4 - - HYPH cord-001257-t21l6i3f 375 5 His33 His33 NNP cord-001257-t21l6i3f 375 6 , , , cord-001257-t21l6i3f 375 7 expressing express VBG cord-001257-t21l6i3f 375 8 the the DT cord-001257-t21l6i3f 375 9 CNV CNV NNP cord-001257-t21l6i3f 375 10 p33 p33 NNP cord-001257-t21l6i3f 375 11 replication replication NN cord-001257-t21l6i3f 375 12 protein protein NN cord-001257-t21l6i3f 375 13 ( ( -LRB- cord-001257-t21l6i3f 375 14 bait bait NN cord-001257-t21l6i3f 375 15 construct construct VB cord-001257-t21l6i3f 375 16 ) ) -RRB- cord-001257-t21l6i3f 375 17 , , , cord-001257-t21l6i3f 375 18 has have VBZ cord-001257-t21l6i3f 375 19 been be VBN cord-001257-t21l6i3f 375 20 published publish VBN cord-001257-t21l6i3f 375 21 earlier early RBR cord-001257-t21l6i3f 375 22 [ [ -LRB- cord-001257-t21l6i3f 375 23 26 26 CD cord-001257-t21l6i3f 375 24 ] ] -RRB- cord-001257-t21l6i3f 375 25 . . . cord-001257-t21l6i3f 376 1 Yeast yeast NN cord-001257-t21l6i3f 376 2 strain strain NN cord-001257-t21l6i3f 377 1 NMY51 NMY51 NNP cord-001257-t21l6i3f 377 2 was be VBD cord-001257-t21l6i3f 377 3 co co JJ cord-001257-t21l6i3f 377 4 - - VBN cord-001257-t21l6i3f 377 5 transformed transform VBN cord-001257-t21l6i3f 377 6 with with IN cord-001257-t21l6i3f 377 7 pGAD pgad JJ cord-001257-t21l6i3f 377 8 - - HYPH cord-001257-t21l6i3f 377 9 BT2-N BT2-N NNP cord-001257-t21l6i3f 377 10 - - HYPH cord-001257-t21l6i3f 377 11 His33 His33 NNP cord-001257-t21l6i3f 377 12 and and CC cord-001257-t21l6i3f 377 13 one one CD cord-001257-t21l6i3f 377 14 of of IN cord-001257-t21l6i3f 377 15 the the DT cord-001257-t21l6i3f 377 16 prey prey NN cord-001257-t21l6i3f 377 17 constructs construct NNS cord-001257-t21l6i3f 377 18 carrying carry VBG cord-001257-t21l6i3f 377 19 the the DT cord-001257-t21l6i3f 377 20 cDNA cdna NN cord-001257-t21l6i3f 377 21 for for IN cord-001257-t21l6i3f 377 22 a a DT cord-001257-t21l6i3f 377 23 given give VBN cord-001257-t21l6i3f 377 24 helicase helicase NN cord-001257-t21l6i3f 377 25 or or CC cord-001257-t21l6i3f 377 26 pPR pPR NNP cord-001257-t21l6i3f 377 27 - - HYPH cord-001257-t21l6i3f 377 28 N N NNP cord-001257-t21l6i3f 377 29 - - HYPH cord-001257-t21l6i3f 377 30 RE RE NNP cord-001257-t21l6i3f 377 31 as as IN cord-001257-t21l6i3f 377 32 a a DT cord-001257-t21l6i3f 377 33 negative negative JJ cord-001257-t21l6i3f 377 34 control control NN cord-001257-t21l6i3f 377 35 or or CC cord-001257-t21l6i3f 377 36 pPR pPR NNP cord-001257-t21l6i3f 377 37 - - HYPH cord-001257-t21l6i3f 377 38 N N NNP cord-001257-t21l6i3f 377 39 - - HYPH cord-001257-t21l6i3f 377 40 SSA1 SSA1 NNP cord-001257-t21l6i3f 377 41 as as IN cord-001257-t21l6i3f 377 42 a a DT cord-001257-t21l6i3f 377 43 positive positive JJ cord-001257-t21l6i3f 377 44 control control NN cord-001257-t21l6i3f 377 45 [ [ -LRB- cord-001257-t21l6i3f 377 46 26 26 CD cord-001257-t21l6i3f 377 47 ] ] -RRB- cord-001257-t21l6i3f 377 48 . . . cord-001257-t21l6i3f 378 1 Yeasts yeast NNS cord-001257-t21l6i3f 378 2 were be VBD cord-001257-t21l6i3f 378 3 plated plate VBN cord-001257-t21l6i3f 378 4 onto onto IN cord-001257-t21l6i3f 378 5 Trp2 Trp2 NNP cord-001257-t21l6i3f 378 6 2 2 CD cord-001257-t21l6i3f 378 7 /Leu2 /leu2 SYM cord-001257-t21l6i3f 378 8 2 2 CD cord-001257-t21l6i3f 378 9 synthetic synthetic JJ cord-001257-t21l6i3f 378 10 minimal minimal JJ cord-001257-t21l6i3f 378 11 medium medium JJ cord-001257-t21l6i3f 378 12 plates plate NNS cord-001257-t21l6i3f 378 13 . . . cord-001257-t21l6i3f 379 1 After after IN cord-001257-t21l6i3f 379 2 transformed transform VBN cord-001257-t21l6i3f 379 3 colonies colony NNS cord-001257-t21l6i3f 379 4 were be VBD cord-001257-t21l6i3f 379 5 picked pick VBN cord-001257-t21l6i3f 379 6 with with IN cord-001257-t21l6i3f 379 7 a a DT cord-001257-t21l6i3f 379 8 loop loop NN cord-001257-t21l6i3f 379 9 and and CC cord-001257-t21l6i3f 379 10 re re NNS cord-001257-t21l6i3f 379 11 - - VBN cord-001257-t21l6i3f 379 12 suspended suspend VBN cord-001257-t21l6i3f 379 13 in in IN cord-001257-t21l6i3f 379 14 water water NN cord-001257-t21l6i3f 379 15 , , , cord-001257-t21l6i3f 379 16 we -PRON- PRP cord-001257-t21l6i3f 379 17 streaked streak VBD cord-001257-t21l6i3f 379 18 them -PRON- PRP cord-001257-t21l6i3f 379 19 onto onto IN cord-001257-t21l6i3f 379 20 TLH TLH NNP cord-001257-t21l6i3f 379 21 2 2 CD cord-001257-t21l6i3f 380 1 ( ( -LRB- cord-001257-t21l6i3f 380 2 Trp2 Trp2 NNP cord-001257-t21l6i3f 380 3 2 2 CD cord-001257-t21l6i3f 380 4 /Leu2 /leu2 SYM cord-001257-t21l6i3f 380 5 2 2 CD cord-001257-t21l6i3f 381 1 /His2 /his2 IN cord-001257-t21l6i3f 381 2 2 2 CD cord-001257-t21l6i3f 381 3 ) ) -RRB- cord-001257-t21l6i3f 381 4 plates plate NNS cord-001257-t21l6i3f 381 5 to to TO cord-001257-t21l6i3f 381 6 test test VB cord-001257-t21l6i3f 381 7 for for IN cord-001257-t21l6i3f 381 8 helicase helicase NN cord-001257-t21l6i3f 381 9 protein protein NN cord-001257-t21l6i3f 381 10 - - HYPH cord-001257-t21l6i3f 381 11 p33 p33 CD cord-001257-t21l6i3f 381 12 interactions interaction NNS cord-001257-t21l6i3f 381 13 as as IN cord-001257-t21l6i3f 381 14 described describe VBN cord-001257-t21l6i3f 381 15 [ [ -LRB- cord-001257-t21l6i3f 381 16 26 26 CD cord-001257-t21l6i3f 381 17 ] ] -RRB- cord-001257-t21l6i3f 381 18 . . . cord-001257-t21l6i3f 382 1 To to TO cord-001257-t21l6i3f 382 2 study study VB cord-001257-t21l6i3f 382 3 the the DT cord-001257-t21l6i3f 382 4 effect effect NN cord-001257-t21l6i3f 382 5 of of IN cord-001257-t21l6i3f 382 6 over over IN cord-001257-t21l6i3f 382 7 - - HYPH cord-001257-t21l6i3f 382 8 expression expression NN cord-001257-t21l6i3f 382 9 of of IN cord-001257-t21l6i3f 382 10 yeast yeast NN cord-001257-t21l6i3f 382 11 and and CC cord-001257-t21l6i3f 382 12 plant plant NN cord-001257-t21l6i3f 382 13 helicase helicase NN cord-001257-t21l6i3f 382 14 proteins protein NNS cord-001257-t21l6i3f 382 15 on on IN cord-001257-t21l6i3f 382 16 DI-72 DI-72 NNP cord-001257-t21l6i3f 382 17 repRNA reprna NN cord-001257-t21l6i3f 382 18 replication replication NN cord-001257-t21l6i3f 382 19 in in IN cord-001257-t21l6i3f 382 20 yeast yeast NN cord-001257-t21l6i3f 382 21 , , , cord-001257-t21l6i3f 382 22 we -PRON- PRP cord-001257-t21l6i3f 382 23 transformed transform VBD cord-001257-t21l6i3f 382 24 S. S. NNP cord-001257-t21l6i3f 382 25 cerevisiae cerevisiae NNS cord-001257-t21l6i3f 382 26 strain strain VB cord-001257-t21l6i3f 382 27 BY4741 BY4741 NNP cord-001257-t21l6i3f 382 28 with with IN cord-001257-t21l6i3f 382 29 three three CD cord-001257-t21l6i3f 382 30 plasmids plasmid NNS cord-001257-t21l6i3f 382 31 : : : cord-001257-t21l6i3f 382 32 pGBK pgbk LS cord-001257-t21l6i3f 382 33 - - HYPH cord-001257-t21l6i3f 382 34 HIS HIS NNP cord-001257-t21l6i3f 382 35 - - HYPH cord-001257-t21l6i3f 382 36 Cup Cup NNP cord-001257-t21l6i3f 382 37 - - HYPH cord-001257-t21l6i3f 382 38 Flag33 Flag33 NNP cord-001257-t21l6i3f 382 39 / / SYM cord-001257-t21l6i3f 382 40 Gal Gal NNP cord-001257-t21l6i3f 382 41 - - HYPH cord-001257-t21l6i3f 382 42 DI-72 DI-72 NNP cord-001257-t21l6i3f 382 43 , , , cord-001257-t21l6i3f 382 44 pGAD pGAD NNP cord-001257-t21l6i3f 382 45 - - HYPH cord-001257-t21l6i3f 382 46 Cup Cup NNP cord-001257-t21l6i3f 382 47 - - HYPH cord-001257-t21l6i3f 382 48 Flag92 Flag92 NNP cord-001257-t21l6i3f 382 49 and and CC cord-001257-t21l6i3f 382 50 one one CD cord-001257-t21l6i3f 382 51 of of IN cord-001257-t21l6i3f 382 52 the the DT cord-001257-t21l6i3f 382 53 following follow VBG cord-001257-t21l6i3f 382 54 plasmids plasmid NNS cord-001257-t21l6i3f 382 55 : : : cord-001257-t21l6i3f 382 56 pYC pyc CD cord-001257-t21l6i3f 382 57 - - HYPH cord-001257-t21l6i3f 382 58 His -PRON- PRP$ cord-001257-t21l6i3f 382 59 - - HYPH cord-001257-t21l6i3f 382 60 RH2 rh2 NN cord-001257-t21l6i3f 382 61 , , , cord-001257-t21l6i3f 382 62 pYC pYC NNP cord-001257-t21l6i3f 382 63 - - HYPH cord-001257-t21l6i3f 382 64 His -PRON- PRP$ cord-001257-t21l6i3f 382 65 - - HYPH cord-001257-t21l6i3f 382 66 RH4 rh4 NN cord-001257-t21l6i3f 382 67 , , , cord-001257-t21l6i3f 382 68 pYC pyc CD cord-001257-t21l6i3f 382 69 - - HYPH cord-001257-t21l6i3f 382 70 His -PRON- PRP$ cord-001257-t21l6i3f 382 71 - - HYPH cord-001257-t21l6i3f 382 72 RH5 rh5 NN cord-001257-t21l6i3f 382 73 , , , cord-001257-t21l6i3f 382 74 pYC pyc CD cord-001257-t21l6i3f 382 75 - - HYPH cord-001257-t21l6i3f 382 76 His -PRON- PRP$ cord-001257-t21l6i3f 382 77 - - HYPH cord-001257-t21l6i3f 382 78 RH7 RH7 NNP cord-001257-t21l6i3f 382 79 , , , cord-001257-t21l6i3f 382 80 pYC pYC NNP cord-001257-t21l6i3f 382 81 - - HYPH cord-001257-t21l6i3f 382 82 His -PRON- PRP$ cord-001257-t21l6i3f 382 83 - - HYPH cord-001257-t21l6i3f 382 84 Dbp3 dbp3 NN cord-001257-t21l6i3f 382 85 , , , cord-001257-t21l6i3f 382 86 pYC pyc CD cord-001257-t21l6i3f 382 87 - - HYPH cord-001257-t21l6i3f 382 88 His -PRON- PRP$ cord-001257-t21l6i3f 382 89 - - HYPH cord-001257-t21l6i3f 382 90 Dbp5 Dbp5 NNP cord-001257-t21l6i3f 382 91 , , , cord-001257-t21l6i3f 382 92 pYC pyc CD cord-001257-t21l6i3f 382 93 - - HYPH cord-001257-t21l6i3f 382 94 His His NNP cord-001257-t21l6i3f 382 95 - - HYPH cord-001257-t21l6i3f 382 96 Dbp7 Dbp7 NNP cord-001257-t21l6i3f 382 97 , , , cord-001257-t21l6i3f 382 98 pYC pyc CD cord-001257-t21l6i3f 382 99 - - HYPH cord-001257-t21l6i3f 382 100 His His NNP cord-001257-t21l6i3f 382 101 - - HYPH cord-001257-t21l6i3f 382 102 Fal1 Fal1 NNP cord-001257-t21l6i3f 382 103 , , , cord-001257-t21l6i3f 382 104 pYC pyc CD cord-001257-t21l6i3f 382 105 - - HYPH cord-001257-t21l6i3f 382 106 His -PRON- PRP$ cord-001257-t21l6i3f 382 107 - - HYPH cord-001257-t21l6i3f 382 108 Tif1 Tif1 NNP cord-001257-t21l6i3f 382 109 ( ( -LRB- cord-001257-t21l6i3f 382 110 empty empty JJ cord-001257-t21l6i3f 382 111 pYC pyc CD cord-001257-t21l6i3f 382 112 plasmid plasmid NN cord-001257-t21l6i3f 382 113 was be VBD cord-001257-t21l6i3f 382 114 used use VBN cord-001257-t21l6i3f 382 115 as as IN cord-001257-t21l6i3f 382 116 a a DT cord-001257-t21l6i3f 382 117 control control NN cord-001257-t21l6i3f 382 118 ) ) -RRB- cord-001257-t21l6i3f 382 119 . . . cord-001257-t21l6i3f 383 1 After after IN cord-001257-t21l6i3f 383 2 the the DT cord-001257-t21l6i3f 383 3 selection selection NN cord-001257-t21l6i3f 383 4 of of IN cord-001257-t21l6i3f 383 5 transformed transform VBN cord-001257-t21l6i3f 383 6 yeast yeast NN cord-001257-t21l6i3f 383 7 cells cell NNS cord-001257-t21l6i3f 383 8 on on IN cord-001257-t21l6i3f 383 9 SC SC NNP cord-001257-t21l6i3f 383 10 - - HYPH cord-001257-t21l6i3f 383 11 ULH ULH NNP cord-001257-t21l6i3f 383 12 2 2 CD cord-001257-t21l6i3f 383 13 plates plate NNS cord-001257-t21l6i3f 383 14 , , , cord-001257-t21l6i3f 383 15 they -PRON- PRP cord-001257-t21l6i3f 383 16 were be VBD cord-001257-t21l6i3f 383 17 pre pre JJ cord-001257-t21l6i3f 383 18 - - VBN cord-001257-t21l6i3f 383 19 grown grown JJ cord-001257-t21l6i3f 383 20 in in IN cord-001257-t21l6i3f 383 21 SC SC NNP cord-001257-t21l6i3f 383 22 - - HYPH cord-001257-t21l6i3f 383 23 ULH ULH NNP cord-001257-t21l6i3f 383 24 2 2 CD cord-001257-t21l6i3f 383 25 media medium NNS cord-001257-t21l6i3f 383 26 containing contain VBG cord-001257-t21l6i3f 383 27 2 2 CD cord-001257-t21l6i3f 383 28 % % NN cord-001257-t21l6i3f 383 29 glucose glucose NN cord-001257-t21l6i3f 383 30 for for IN cord-001257-t21l6i3f 383 31 24 24 CD cord-001257-t21l6i3f 383 32 h h NN cord-001257-t21l6i3f 383 33 at at IN cord-001257-t21l6i3f 383 34 29uC. 29uc. CD cord-001257-t21l6i3f 384 1 Then then RB cord-001257-t21l6i3f 384 2 cells cell NNS cord-001257-t21l6i3f 384 3 were be VBD cord-001257-t21l6i3f 384 4 centrifuged centrifuge VBN cord-001257-t21l6i3f 384 5 at at IN cord-001257-t21l6i3f 384 6 2,000 2,000 CD cord-001257-t21l6i3f 384 7 rpm rpm NN cord-001257-t21l6i3f 384 8 for for IN cord-001257-t21l6i3f 384 9 3 3 CD cord-001257-t21l6i3f 384 10 min min NN cord-001257-t21l6i3f 384 11 , , , cord-001257-t21l6i3f 384 12 washed wash VBN cord-001257-t21l6i3f 384 13 with with IN cord-001257-t21l6i3f 384 14 SC SC NNP cord-001257-t21l6i3f 384 15 - - HYPH cord-001257-t21l6i3f 384 16 ULH ULH NNP cord-001257-t21l6i3f 384 17 2 2 CD cord-001257-t21l6i3f 384 18 media medium NNS cord-001257-t21l6i3f 384 19 containing contain VBG cord-001257-t21l6i3f 384 20 2 2 CD cord-001257-t21l6i3f 384 21 % % NN cord-001257-t21l6i3f 384 22 galactose galactose NN cord-001257-t21l6i3f 384 23 and and CC cord-001257-t21l6i3f 384 24 resuspended resuspend VBD cord-001257-t21l6i3f 384 25 in in IN cord-001257-t21l6i3f 384 26 SC SC NNP cord-001257-t21l6i3f 384 27 - - HYPH cord-001257-t21l6i3f 384 28 ULH ULH NNP cord-001257-t21l6i3f 384 29 2 2 CD cord-001257-t21l6i3f 384 30 media medium NNS cord-001257-t21l6i3f 384 31 containing contain VBG cord-001257-t21l6i3f 384 32 2 2 CD cord-001257-t21l6i3f 384 33 % % NN cord-001257-t21l6i3f 384 34 galactose galactose NN cord-001257-t21l6i3f 384 35 and and CC cord-001257-t21l6i3f 384 36 50 50 CD cord-001257-t21l6i3f 384 37 mM mm NN cord-001257-t21l6i3f 384 38 CuSO CuSO NNP cord-001257-t21l6i3f 384 39 4 4 CD cord-001257-t21l6i3f 384 40 . . . cord-001257-t21l6i3f 385 1 After after IN cord-001257-t21l6i3f 385 2 growing grow VBG cord-001257-t21l6i3f 385 3 yeast yeast NN cord-001257-t21l6i3f 385 4 cells cell NNS cord-001257-t21l6i3f 385 5 for for IN cord-001257-t21l6i3f 385 6 14 14 CD cord-001257-t21l6i3f 385 7 h h NN cord-001257-t21l6i3f 385 8 at at IN cord-001257-t21l6i3f 385 9 23uC 23uc CD cord-001257-t21l6i3f 385 10 , , , cord-001257-t21l6i3f 385 11 they -PRON- PRP cord-001257-t21l6i3f 385 12 were be VBD cord-001257-t21l6i3f 385 13 used use VBN cord-001257-t21l6i3f 385 14 for for IN cord-001257-t21l6i3f 385 15 total total JJ cord-001257-t21l6i3f 385 16 RNA RNA NNP cord-001257-t21l6i3f 385 17 extraction extraction NN cord-001257-t21l6i3f 385 18 and and CC cord-001257-t21l6i3f 385 19 Northern northern JJ cord-001257-t21l6i3f 385 20 blotting blotting NN cord-001257-t21l6i3f 385 21 and and CC cord-001257-t21l6i3f 385 22 Western western JJ cord-001257-t21l6i3f 385 23 blotting blotting NN cord-001257-t21l6i3f 385 24 as as IN cord-001257-t21l6i3f 385 25 previously previously RB cord-001257-t21l6i3f 385 26 published publish VBN cord-001257-t21l6i3f 385 27 [ [ -LRB- cord-001257-t21l6i3f 385 28 15 15 CD cord-001257-t21l6i3f 385 29 ] ] -RRB- cord-001257-t21l6i3f 385 30 . . . cord-001257-t21l6i3f 386 1 After after IN cord-001257-t21l6i3f 386 2 yeast yeast NN cord-001257-t21l6i3f 386 3 strain strain VBP cord-001257-t21l6i3f 386 4 TET TET NNP cord-001257-t21l6i3f 386 5 : : : cord-001257-t21l6i3f 387 1 Fal1 Fal1 NNP cord-001257-t21l6i3f 387 2 was be VBD cord-001257-t21l6i3f 387 3 transformed transform VBN cord-001257-t21l6i3f 387 4 with with IN cord-001257-t21l6i3f 387 5 plasmids plasmid NNS cord-001257-t21l6i3f 387 6 pGBK pgbk JJ cord-001257-t21l6i3f 387 7 - - HYPH cord-001257-t21l6i3f 387 8 HIS HIS NNP cord-001257-t21l6i3f 387 9 - - HYPH cord-001257-t21l6i3f 387 10 Cup Cup NNP cord-001257-t21l6i3f 387 11 - - HYPH cord-001257-t21l6i3f 387 12 Flag33 Flag33 NNP cord-001257-t21l6i3f 387 13 / / SYM cord-001257-t21l6i3f 387 14 Gal Gal NNP cord-001257-t21l6i3f 387 15 - - HYPH cord-001257-t21l6i3f 387 16 DI-72 DI-72 NNP cord-001257-t21l6i3f 387 17 and and CC cord-001257-t21l6i3f 387 18 pGAD pGAD NNP cord-001257-t21l6i3f 387 19 - - HYPH cord-001257-t21l6i3f 387 20 Cup Cup NNP cord-001257-t21l6i3f 387 21 - - HYPH cord-001257-t21l6i3f 387 22 Flag92 Flag92 NNP cord-001257-t21l6i3f 387 23 , , , cord-001257-t21l6i3f 387 24 it -PRON- PRP cord-001257-t21l6i3f 387 25 was be VBD cord-001257-t21l6i3f 387 26 pre pre JJ cord-001257-t21l6i3f 387 27 - - VBN cord-001257-t21l6i3f 387 28 grown grown JJ cord-001257-t21l6i3f 387 29 in in IN cord-001257-t21l6i3f 387 30 SC SC NNP cord-001257-t21l6i3f 387 31 - - HYPH cord-001257-t21l6i3f 387 32 ULH ULH NNP cord-001257-t21l6i3f 387 33 2 2 CD cord-001257-t21l6i3f 387 34 media medium NNS cord-001257-t21l6i3f 387 35 containing contain VBG cord-001257-t21l6i3f 387 36 2 2 CD cord-001257-t21l6i3f 387 37 % % NN cord-001257-t21l6i3f 387 38 glucose glucose NN cord-001257-t21l6i3f 387 39 at at IN cord-001257-t21l6i3f 387 40 29uC. 29uc. CD cord-001257-t21l6i3f 388 1 Then then RB cord-001257-t21l6i3f 388 2 yeast yeast NN cord-001257-t21l6i3f 388 3 cells cell NNS cord-001257-t21l6i3f 388 4 were be VBD cord-001257-t21l6i3f 388 5 centrifuged centrifuge VBN cord-001257-t21l6i3f 388 6 at at IN cord-001257-t21l6i3f 388 7 2,000 2,000 CD cord-001257-t21l6i3f 388 8 rpm rpm NN cord-001257-t21l6i3f 388 9 for for IN cord-001257-t21l6i3f 388 10 3 3 CD cord-001257-t21l6i3f 388 11 min min NN cord-001257-t21l6i3f 388 12 , , , cord-001257-t21l6i3f 388 13 washed wash VBN cord-001257-t21l6i3f 388 14 with with IN cord-001257-t21l6i3f 388 15 SC SC NNP cord-001257-t21l6i3f 388 16 - - HYPH cord-001257-t21l6i3f 388 17 ULH ULH NNP cord-001257-t21l6i3f 388 18 2 2 CD cord-001257-t21l6i3f 388 19 media medium NNS cord-001257-t21l6i3f 388 20 containing contain VBG cord-001257-t21l6i3f 388 21 2 2 CD cord-001257-t21l6i3f 388 22 % % NN cord-001257-t21l6i3f 388 23 galactose galactose NN cord-001257-t21l6i3f 388 24 and and CC cord-001257-t21l6i3f 388 25 resuspended resuspend VBD cord-001257-t21l6i3f 388 26 in in IN cord-001257-t21l6i3f 388 27 SC SC NNP cord-001257-t21l6i3f 388 28 - - HYPH cord-001257-t21l6i3f 388 29 ULH ULH NNP cord-001257-t21l6i3f 388 30 2 2 CD cord-001257-t21l6i3f 388 31 media medium NNS cord-001257-t21l6i3f 388 32 containing contain VBG cord-001257-t21l6i3f 388 33 2 2 CD cord-001257-t21l6i3f 388 34 % % NN cord-001257-t21l6i3f 388 35 galactose galactose NN cord-001257-t21l6i3f 388 36 and and CC cord-001257-t21l6i3f 388 37 50 50 CD cord-001257-t21l6i3f 388 38 mM mm NN cord-001257-t21l6i3f 388 39 CuSO CuSO NNP cord-001257-t21l6i3f 388 40 4 4 CD cord-001257-t21l6i3f 388 41 in in IN cord-001257-t21l6i3f 388 42 the the DT cord-001257-t21l6i3f 388 43 presence presence NN cord-001257-t21l6i3f 388 44 or or CC cord-001257-t21l6i3f 388 45 absence absence NN cord-001257-t21l6i3f 388 46 of of IN cord-001257-t21l6i3f 388 47 1 1 CD cord-001257-t21l6i3f 388 48 mg mg NNP cord-001257-t21l6i3f 388 49 / / SYM cord-001257-t21l6i3f 388 50 ml ml NNP cord-001257-t21l6i3f 388 51 Doxycycline Doxycycline NNP cord-001257-t21l6i3f 388 52 . . . cord-001257-t21l6i3f 389 1 After after IN cord-001257-t21l6i3f 389 2 growing grow VBG cord-001257-t21l6i3f 389 3 for for IN cord-001257-t21l6i3f 389 4 24 24 CD cord-001257-t21l6i3f 389 5 h h NN cord-001257-t21l6i3f 389 6 at at IN cord-001257-t21l6i3f 389 7 23uC 23uc CD cord-001257-t21l6i3f 389 8 , , , cord-001257-t21l6i3f 389 9 and and CC cord-001257-t21l6i3f 389 10 yeasts yeast NNS cord-001257-t21l6i3f 389 11 were be VBD cord-001257-t21l6i3f 389 12 pelleted pellete VBN cord-001257-t21l6i3f 389 13 and and CC cord-001257-t21l6i3f 389 14 the the DT cord-001257-t21l6i3f 389 15 replicase replicase NN cord-001257-t21l6i3f 389 16 was be VBD cord-001257-t21l6i3f 389 17 purified purify VBN cord-001257-t21l6i3f 389 18 according accord VBG cord-001257-t21l6i3f 389 19 to to IN cord-001257-t21l6i3f 389 20 a a DT cord-001257-t21l6i3f 389 21 previously previously RB cord-001257-t21l6i3f 389 22 published publish VBN cord-001257-t21l6i3f 389 23 procedure procedure NN cord-001257-t21l6i3f 389 24 [ [ -LRB- cord-001257-t21l6i3f 389 25 25 25 CD cord-001257-t21l6i3f 389 26 ] ] -RRB- cord-001257-t21l6i3f 389 27 . . . cord-001257-t21l6i3f 390 1 Briefly briefly RB cord-001257-t21l6i3f 390 2 , , , cord-001257-t21l6i3f 390 3 approximately approximately RB cord-001257-t21l6i3f 390 4 200 200 CD cord-001257-t21l6i3f 390 5 mg mg NNS cord-001257-t21l6i3f 390 6 of of IN cord-001257-t21l6i3f 390 7 wet wet JJ cord-001257-t21l6i3f 390 8 yeast yeast NN cord-001257-t21l6i3f 390 9 cell cell NN cord-001257-t21l6i3f 390 10 pellet pellet NN cord-001257-t21l6i3f 390 11 were be VBD cord-001257-t21l6i3f 390 12 resuspended resuspend VBN cord-001257-t21l6i3f 390 13 in in IN cord-001257-t21l6i3f 390 14 TG TG NNP cord-001257-t21l6i3f 390 15 buffer buffer NN cord-001257-t21l6i3f 390 16 [ [ -LRB- cord-001257-t21l6i3f 390 17 In in FW cord-001257-t21l6i3f 390 18 vitro vitro FW cord-001257-t21l6i3f 390 19 TBSV TBSV NNP cord-001257-t21l6i3f 390 20 replication replication NN cord-001257-t21l6i3f 390 21 assay assay NN cord-001257-t21l6i3f 390 22 in in IN cord-001257-t21l6i3f 390 23 cell cell NN cord-001257-t21l6i3f 390 24 - - HYPH cord-001257-t21l6i3f 390 25 free free JJ cord-001257-t21l6i3f 390 26 yeast yeast NN cord-001257-t21l6i3f 390 27 extract extract NN cord-001257-t21l6i3f 391 1 We -PRON- PRP cord-001257-t21l6i3f 391 2 prepared prepare VBD cord-001257-t21l6i3f 391 3 cell cell NN cord-001257-t21l6i3f 391 4 - - HYPH cord-001257-t21l6i3f 391 5 free free JJ cord-001257-t21l6i3f 391 6 extract extract NN cord-001257-t21l6i3f 391 7 ( ( -LRB- cord-001257-t21l6i3f 391 8 CFE CFE NNP cord-001257-t21l6i3f 391 9 ) ) -RRB- cord-001257-t21l6i3f 391 10 from from IN cord-001257-t21l6i3f 391 11 BY4741 BY4741 NNP cord-001257-t21l6i3f 391 12 or or CC cord-001257-t21l6i3f 391 13 Ddbp3 ddbp3 NN cord-001257-t21l6i3f 391 14 / / SYM cord-001257-t21l6i3f 391 15 ts ts JJ cord-001257-t21l6i3f 391 16 - - HYPH cord-001257-t21l6i3f 391 17 Fal1 fal1 JJ cord-001257-t21l6i3f 391 18 yeast yeast NN cord-001257-t21l6i3f 391 19 strains strain NNS cord-001257-t21l6i3f 391 20 as as IN cord-001257-t21l6i3f 391 21 described describe VBN cord-001257-t21l6i3f 391 22 earlier early RBR cord-001257-t21l6i3f 391 23 [ [ -LRB- cord-001257-t21l6i3f 391 24 24 24 CD cord-001257-t21l6i3f 391 25 ] ] -RRB- cord-001257-t21l6i3f 391 26 . . . cord-001257-t21l6i3f 392 1 The the DT cord-001257-t21l6i3f 392 2 CFE CFE NNP cord-001257-t21l6i3f 392 3 - - HYPH cord-001257-t21l6i3f 392 4 based base VBN cord-001257-t21l6i3f 392 5 TBSV TBSV NNP cord-001257-t21l6i3f 392 6 replication replication NN cord-001257-t21l6i3f 392 7 assays assay NNS cord-001257-t21l6i3f 392 8 were be VBD cord-001257-t21l6i3f 392 9 performed perform VBN cord-001257-t21l6i3f 392 10 in in IN cord-001257-t21l6i3f 392 11 20 20 CD cord-001257-t21l6i3f 392 12 ml ml NN cord-001257-t21l6i3f 392 13 total total JJ cord-001257-t21l6i3f 392 14 volume volume NN cord-001257-t21l6i3f 392 15 containing contain VBG cord-001257-t21l6i3f 392 16 2 2 CD cord-001257-t21l6i3f 392 17 ml ml NNS cord-001257-t21l6i3f 392 18 of of IN cord-001257-t21l6i3f 392 19 CFE CFE NNP cord-001257-t21l6i3f 392 20 , , , cord-001257-t21l6i3f 392 21 unlabeled unlabeled JJ cord-001257-t21l6i3f 393 1 0.15 0.15 CD cord-001257-t21l6i3f 393 2 mg mg NNP cord-001257-t21l6i3f 393 3 DI-72 DI-72 NNP cord-001257-t21l6i3f 393 4 ( ( -LRB- cord-001257-t21l6i3f 393 5 + + NN cord-001257-t21l6i3f 393 6 ) ) -RRB- cord-001257-t21l6i3f 393 7 RNA RNA NNP cord-001257-t21l6i3f 393 8 or or CC cord-001257-t21l6i3f 393 9 RI RI NNP cord-001257-t21l6i3f 393 10 / / SYM cord-001257-t21l6i3f 393 11 II II NNP cord-001257-t21l6i3f 393 12 / / SYM cord-001257-t21l6i3f 393 13 IV IV NNP cord-001257-t21l6i3f 393 14 ( ( -LRB- cord-001257-t21l6i3f 393 15 + + CD cord-001257-t21l6i3f 393 16 ) ) -RRB- cord-001257-t21l6i3f 393 17 RNA RNA NNP cord-001257-t21l6i3f 393 18 transcripts transcript NNS cord-001257-t21l6i3f 393 19 , , , cord-001257-t21l6i3f 393 20 200 200 CD cord-001257-t21l6i3f 393 21 ng ng NN cord-001257-t21l6i3f 393 22 purified purify VBN cord-001257-t21l6i3f 393 23 MBP MBP NNP cord-001257-t21l6i3f 393 24 - - HYPH cord-001257-t21l6i3f 393 25 p33 p33 CD cord-001257-t21l6i3f 393 26 , , , cord-001257-t21l6i3f 393 27 200 200 CD cord-001257-t21l6i3f 393 28 ng ng NN cord-001257-t21l6i3f 393 29 purified purify VBN cord-001257-t21l6i3f 393 30 MBP MBP NNP cord-001257-t21l6i3f 393 31 - - HYPH cord-001257-t21l6i3f 393 32 p92 p92 NN cord-001257-t21l6i3f 393 33 pol pol NN cord-001257-t21l6i3f 393 34 and and CC cord-001257-t21l6i3f 393 35 200 200 CD cord-001257-t21l6i3f 393 36 ng ng NN cord-001257-t21l6i3f 393 37 purified purify VBN cord-001257-t21l6i3f 393 38 MBP MBP NNP cord-001257-t21l6i3f 393 39 - - HYPH cord-001257-t21l6i3f 393 40 tagged tag VBN cord-001257-t21l6i3f 393 41 helicase helicase NN cord-001257-t21l6i3f 393 42 proteins protein NNS cord-001257-t21l6i3f 393 43 . . . cord-001257-t21l6i3f 394 1 The the DT cord-001257-t21l6i3f 394 2 assays assay NNS cord-001257-t21l6i3f 394 3 were be VBD cord-001257-t21l6i3f 394 4 performed perform VBN cord-001257-t21l6i3f 394 5 as as IN cord-001257-t21l6i3f 394 6 published publish VBN cord-001257-t21l6i3f 394 7 [ [ -LRB- cord-001257-t21l6i3f 394 8 24 24 CD cord-001257-t21l6i3f 394 9 , , , cord-001257-t21l6i3f 394 10 63 63 CD cord-001257-t21l6i3f 394 11 ] ] -RRB- cord-001257-t21l6i3f 394 12 . . . cord-001257-t21l6i3f 395 1 Fractionation fractionation NN cord-001257-t21l6i3f 395 2 of of IN cord-001257-t21l6i3f 395 3 the the DT cord-001257-t21l6i3f 395 4 assay assay NN cord-001257-t21l6i3f 395 5 products product NNS cord-001257-t21l6i3f 395 6 was be VBD cord-001257-t21l6i3f 395 7 done do VBN cord-001257-t21l6i3f 395 8 as as IN cord-001257-t21l6i3f 395 9 follows follow VBZ cord-001257-t21l6i3f 395 10 : : : cord-001257-t21l6i3f 395 11 after after IN cord-001257-t21l6i3f 395 12 3 3 CD cord-001257-t21l6i3f 395 13 h h NN cord-001257-t21l6i3f 395 14 of of IN cord-001257-t21l6i3f 395 15 incubation incubation NN cord-001257-t21l6i3f 395 16 at at IN cord-001257-t21l6i3f 395 17 25uC 25uc CD cord-001257-t21l6i3f 395 18 , , , cord-001257-t21l6i3f 395 19 reaction reaction NN cord-001257-t21l6i3f 395 20 mixtures mixture NNS cord-001257-t21l6i3f 395 21 were be VBD cord-001257-t21l6i3f 395 22 centrifuged centrifuge VBN cord-001257-t21l6i3f 395 23 at at IN cord-001257-t21l6i3f 395 24 21,0006 21,0006 NNP cord-001257-t21l6i3f 395 25 g g NNP cord-001257-t21l6i3f 395 26 for for IN cord-001257-t21l6i3f 395 27 10 10 CD cord-001257-t21l6i3f 395 28 min min NN cord-001257-t21l6i3f 395 29 to to TO cord-001257-t21l6i3f 395 30 separate separate VB cord-001257-t21l6i3f 395 31 the the DT cord-001257-t21l6i3f 395 32 ' ' `` cord-001257-t21l6i3f 395 33 ' ' `` cord-001257-t21l6i3f 395 34 soluble soluble JJ cord-001257-t21l6i3f 395 35 ' ' '' cord-001257-t21l6i3f 395 36 ' ' '' cord-001257-t21l6i3f 395 37 ( ( -LRB- cord-001257-t21l6i3f 395 38 supernatant supernatant JJ cord-001257-t21l6i3f 395 39 ) ) -RRB- cord-001257-t21l6i3f 395 40 and and CC cord-001257-t21l6i3f 395 41 ' ' `` cord-001257-t21l6i3f 395 42 ' ' `` cord-001257-t21l6i3f 395 43 membrane membrane NN cord-001257-t21l6i3f 395 44 ' ' '' cord-001257-t21l6i3f 395 45 ' ' '' cord-001257-t21l6i3f 395 46 ( ( -LRB- cord-001257-t21l6i3f 395 47 pellet pellet NN cord-001257-t21l6i3f 395 48 ) ) -RRB- cord-001257-t21l6i3f 395 49 fraction fraction NN cord-001257-t21l6i3f 395 50 . . . cord-001257-t21l6i3f 396 1 Then then RB cord-001257-t21l6i3f 396 2 the the DT cord-001257-t21l6i3f 396 3 membrane membrane NN cord-001257-t21l6i3f 396 4 fraction fraction NN cord-001257-t21l6i3f 396 5 was be VBD cord-001257-t21l6i3f 396 6 re re VBN cord-001257-t21l6i3f 396 7 - - VBN cord-001257-t21l6i3f 396 8 suspended suspend VBN cord-001257-t21l6i3f 396 9 in in IN cord-001257-t21l6i3f 396 10 reaction reaction NN cord-001257-t21l6i3f 396 11 buffer buffer NN cord-001257-t21l6i3f 396 12 . . . cord-001257-t21l6i3f 397 1 Both both DT cord-001257-t21l6i3f 397 2 fractions fraction NNS cord-001257-t21l6i3f 397 3 were be VBD cord-001257-t21l6i3f 397 4 then then RB cord-001257-t21l6i3f 397 5 treated treat VBN cord-001257-t21l6i3f 397 6 as as IN cord-001257-t21l6i3f 397 7 separate separate JJ cord-001257-t21l6i3f 397 8 samples sample NNS cord-001257-t21l6i3f 397 9 during during IN cord-001257-t21l6i3f 397 10 phenol phenol NN cord-001257-t21l6i3f 397 11 / / SYM cord-001257-t21l6i3f 397 12 chlorophorm chlorophorm NN cord-001257-t21l6i3f 397 13 extraction extraction NN cord-001257-t21l6i3f 397 14 , , , cord-001257-t21l6i3f 397 15 ethanol ethanol NN cord-001257-t21l6i3f 397 16 precipitation precipitation NN cord-001257-t21l6i3f 397 17 . . . cord-001257-t21l6i3f 398 1 The the DT cord-001257-t21l6i3f 398 2 samples sample NNS cord-001257-t21l6i3f 398 3 were be VBD cord-001257-t21l6i3f 398 4 dissolved dissolve VBN cord-001257-t21l6i3f 398 5 in in IN cord-001257-t21l6i3f 398 6 16RNA 16rna CD cord-001257-t21l6i3f 398 7 loading loading NN cord-001257-t21l6i3f 398 8 dye dye NN cord-001257-t21l6i3f 398 9 and and CC cord-001257-t21l6i3f 398 10 analyzed analyze VBN cord-001257-t21l6i3f 398 11 by by IN cord-001257-t21l6i3f 398 12 PAGE page NN cord-001257-t21l6i3f 398 13 electrophoresis electrophoresis NN cord-001257-t21l6i3f 398 14 in in IN cord-001257-t21l6i3f 398 15 5 5 CD cord-001257-t21l6i3f 398 16 % % NN cord-001257-t21l6i3f 398 17 polyacrylamide polyacrylamide NN cord-001257-t21l6i3f 398 18 gel gel NN cord-001257-t21l6i3f 398 19 containing contain VBG cord-001257-t21l6i3f 398 20 8 8 CD cord-001257-t21l6i3f 398 21 M M NNP cord-001257-t21l6i3f 398 22 urea urea NN cord-001257-t21l6i3f 398 23 with with IN cord-001257-t21l6i3f 398 24 0.56 0.56 CD cord-001257-t21l6i3f 398 25 Tris Tris NNP cord-001257-t21l6i3f 398 26 - - HYPH cord-001257-t21l6i3f 398 27 borate borate NNP cord-001257-t21l6i3f 398 28 / / SYM cord-001257-t21l6i3f 398 29 EDTA EDTA NNP cord-001257-t21l6i3f 398 30 buffer buffer NN cord-001257-t21l6i3f 398 31 as as IN cord-001257-t21l6i3f 398 32 described describe VBN cord-001257-t21l6i3f 398 33 [ [ -LRB- cord-001257-t21l6i3f 398 34 24 24 CD cord-001257-t21l6i3f 398 35 , , , cord-001257-t21l6i3f 398 36 63 63 CD cord-001257-t21l6i3f 398 37 ] ] -RRB- cord-001257-t21l6i3f 398 38 . . . cord-001257-t21l6i3f 399 1 For for IN cord-001257-t21l6i3f 399 2 the the DT cord-001257-t21l6i3f 399 3 detection detection NN cord-001257-t21l6i3f 399 4 of of IN cord-001257-t21l6i3f 399 5 the the DT cord-001257-t21l6i3f 399 6 32 32 CD cord-001257-t21l6i3f 399 7 P p NN cord-001257-t21l6i3f 399 8 - - HYPH cord-001257-t21l6i3f 399 9 labeled label VBN cord-001257-t21l6i3f 399 10 dsRNAs dsRNAs NNP cord-001257-t21l6i3f 399 11 generated generate VBN cord-001257-t21l6i3f 399 12 in in IN cord-001257-t21l6i3f 399 13 the the DT cord-001257-t21l6i3f 399 14 CFE CFE NNP cord-001257-t21l6i3f 399 15 assays assay NNS cord-001257-t21l6i3f 399 16 , , , cord-001257-t21l6i3f 399 17 we -PRON- PRP cord-001257-t21l6i3f 399 18 prepared prepare VBD cord-001257-t21l6i3f 399 19 the the DT cord-001257-t21l6i3f 399 20 RNA RNA NNP cord-001257-t21l6i3f 399 21 samples sample NNS cord-001257-t21l6i3f 399 22 in in IN cord-001257-t21l6i3f 399 23 16 16 CD cord-001257-t21l6i3f 399 24 RNA RNA NNP cord-001257-t21l6i3f 399 25 loading loading NN cord-001257-t21l6i3f 399 26 dye dye NN cord-001257-t21l6i3f 399 27 ( ( -LRB- cord-001257-t21l6i3f 399 28 containing contain VBG cord-001257-t21l6i3f 399 29 25 25 CD cord-001257-t21l6i3f 399 30 % % NN cord-001257-t21l6i3f 399 31 formamide formamide JJ cord-001257-t21l6i3f 399 32 ) ) -RRB- cord-001257-t21l6i3f 399 33 , , , cord-001257-t21l6i3f 399 34 followed follow VBN cord-001257-t21l6i3f 399 35 by by IN cord-001257-t21l6i3f 399 36 dividing divide VBG cord-001257-t21l6i3f 399 37 the the DT cord-001257-t21l6i3f 399 38 samples sample NNS cord-001257-t21l6i3f 399 39 into into IN cord-001257-t21l6i3f 399 40 two two CD cord-001257-t21l6i3f 399 41 equal equal JJ cord-001257-t21l6i3f 399 42 fractions fraction NNS cord-001257-t21l6i3f 399 43 ; ; : cord-001257-t21l6i3f 399 44 one one CD cord-001257-t21l6i3f 399 45 half half NN cord-001257-t21l6i3f 399 46 was be VBD cord-001257-t21l6i3f 399 47 loaded load VBN cord-001257-t21l6i3f 399 48 on on IN cord-001257-t21l6i3f 399 49 the the DT cord-001257-t21l6i3f 399 50 gel gel NN cord-001257-t21l6i3f 399 51 without without IN cord-001257-t21l6i3f 399 52 heat heat NN cord-001257-t21l6i3f 399 53 - - HYPH cord-001257-t21l6i3f 399 54 treatment treatment NN cord-001257-t21l6i3f 399 55 , , , cord-001257-t21l6i3f 399 56 while while IN cord-001257-t21l6i3f 399 57 the the DT cord-001257-t21l6i3f 399 58 other other JJ cord-001257-t21l6i3f 399 59 half half NN cord-001257-t21l6i3f 399 60 was be VBD cord-001257-t21l6i3f 399 61 heattreated heattreate VBN cord-001257-t21l6i3f 399 62 for for IN cord-001257-t21l6i3f 399 63 RNA RNA NNP cord-001257-t21l6i3f 399 64 denaturation denaturation NN cord-001257-t21l6i3f 399 65 at at IN cord-001257-t21l6i3f 399 66 85uC 85uc CD cord-001257-t21l6i3f 399 67 for for IN cord-001257-t21l6i3f 399 68 5 5 CD cord-001257-t21l6i3f 399 69 min min NN cord-001257-t21l6i3f 399 70 and and CC cord-001257-t21l6i3f 399 71 analyzed analyze VBN cord-001257-t21l6i3f 399 72 by by IN cord-001257-t21l6i3f 399 73 PAGE page NN cord-001257-t21l6i3f 399 74 [ [ -LRB- cord-001257-t21l6i3f 399 75 27 27 CD cord-001257-t21l6i3f 399 76 ] ] -RRB- cord-001257-t21l6i3f 399 77 . . . cord-001257-t21l6i3f 400 1 in in IN cord-001257-t21l6i3f 400 2 the the DT cord-001257-t21l6i3f 400 3 presence presence NN cord-001257-t21l6i3f 400 4 of of IN cord-001257-t21l6i3f 400 5 0.4 0.4 CD cord-001257-t21l6i3f 400 6 mg mg NN cord-001257-t21l6i3f 400 7 of of IN cord-001257-t21l6i3f 400 8 purified purify VBN cord-001257-t21l6i3f 400 9 recombinant recombinant JJ cord-001257-t21l6i3f 400 10 cellular cellular JJ cord-001257-t21l6i3f 400 11 helicases helicase NNS cord-001257-t21l6i3f 400 12 ( ( -LRB- cord-001257-t21l6i3f 400 13 except except IN cord-001257-t21l6i3f 400 14 1.0 1.0 CD cord-001257-t21l6i3f 400 15 mg mg NN cord-001257-t21l6i3f 400 16 in in IN cord-001257-t21l6i3f 400 17 case case NN cord-001257-t21l6i3f 400 18 of of IN cord-001257-t21l6i3f 400 19 AtRH20 atrh20 NN cord-001257-t21l6i3f 400 20 ) ) -RRB- cord-001257-t21l6i3f 400 21 is be VBZ cord-001257-t21l6i3f 400 22 shown show VBN cord-001257-t21l6i3f 400 23 . . . cord-001257-t21l6i3f 401 1 Note note VB cord-001257-t21l6i3f 401 2 that that IN cord-001257-t21l6i3f 401 3 lanes lane NNS cord-001257-t21l6i3f 401 4 1 1 CD cord-001257-t21l6i3f 401 5 - - SYM cord-001257-t21l6i3f 401 6 6 6 CD cord-001257-t21l6i3f 401 7 show show NN cord-001257-t21l6i3f 401 8 samples sample NNS cord-001257-t21l6i3f 401 9 from from IN cord-001257-t21l6i3f 401 10 the the DT cord-001257-t21l6i3f 401 11 in in FW cord-001257-t21l6i3f 401 12 vitro vitro FW cord-001257-t21l6i3f 401 13 replication replication NN cord-001257-t21l6i3f 401 14 assays assay NNS cord-001257-t21l6i3f 401 15 with with IN cord-001257-t21l6i3f 401 16 the the DT cord-001257-t21l6i3f 401 17 combination combination NN cord-001257-t21l6i3f 401 18 of of IN cord-001257-t21l6i3f 401 19 two two CD cord-001257-t21l6i3f 401 20 cellular cellular JJ cord-001257-t21l6i3f 401 21 helicases helicase NNS cord-001257-t21l6i3f 401 22 [ [ -LRB- cord-001257-t21l6i3f 401 23 i.e. i.e. FW cord-001257-t21l6i3f 401 24 , , , cord-001257-t21l6i3f 401 25 AtRH20 AtRH20 NNP cord-001257-t21l6i3f 401 26 ( ( -LRB- cord-001257-t21l6i3f 401 27 1.0 1.0 CD cord-001257-t21l6i3f 401 28 mg mg NNP cord-001257-t21l6i3f 401 29 ) ) -RRB- cord-001257-t21l6i3f 401 30 plus plus CC cord-001257-t21l6i3f 401 31 the the DT cord-001257-t21l6i3f 401 32 shown show VBN cord-001257-t21l6i3f 401 33 helicase helicase NN cord-001257-t21l6i3f 401 34 ) ) -RRB- cord-001257-t21l6i3f 401 35 , , , cord-001257-t21l6i3f 401 36 while while IN cord-001257-t21l6i3f 401 37 lanes lane NNS cord-001257-t21l6i3f 401 38 7 7 CD cord-001257-t21l6i3f 401 39 - - SYM cord-001257-t21l6i3f 401 40 13 13 CD cord-001257-t21l6i3f 401 41 show show NN cord-001257-t21l6i3f 401 42 samples sample NNS cord-001257-t21l6i3f 401 43 with with IN cord-001257-t21l6i3f 401 44 only only RB cord-001257-t21l6i3f 401 45 a a DT cord-001257-t21l6i3f 401 46 single single JJ cord-001257-t21l6i3f 401 47 helicase helicase NN cord-001257-t21l6i3f 401 48 in in IN cord-001257-t21l6i3f 401 49 the the DT cord-001257-t21l6i3f 401 50 assay assay NN cord-001257-t21l6i3f 401 51 . . . cord-001257-t21l6i3f 402 1 ( ( -LRB- cord-001257-t21l6i3f 402 2 C C NNP cord-001257-t21l6i3f 402 3 ) ) -RRB- cord-001257-t21l6i3f 402 4 Detection detection NN cord-001257-t21l6i3f 402 5 of of IN cord-001257-t21l6i3f 402 6 ( ( -LRB- cord-001257-t21l6i3f 402 7 + + NN cord-001257-t21l6i3f 402 8 ) ) -RRB- cord-001257-t21l6i3f 402 9 and and CC cord-001257-t21l6i3f 402 10 ( ( -LRB- cord-001257-t21l6i3f 402 11 2)-stranded 2)-stranded CD cord-001257-t21l6i3f 402 12 RNA RNA NNP cord-001257-t21l6i3f 402 13 products product NNS cord-001257-t21l6i3f 402 14 produced produce VBN cord-001257-t21l6i3f 402 15 by by IN cord-001257-t21l6i3f 402 16 the the DT cord-001257-t21l6i3f 402 17 purified purify VBN cord-001257-t21l6i3f 402 18 TBSV TBSV NNP cord-001257-t21l6i3f 402 19 replicase replicase NN cord-001257-t21l6i3f 402 20 on on IN cord-001257-t21l6i3f 402 21 the the DT cord-001257-t21l6i3f 402 22 DI-72 DI-72 NNP cord-001257-t21l6i3f 402 23 RNA RNA NNP cord-001257-t21l6i3f 402 24 / / SYM cord-001257-t21l6i3f 402 25 RNA RNA NNP cord-001257-t21l6i3f 402 26 duplex duplex NN cord-001257-t21l6i3f 402 27 template template NN cord-001257-t21l6i3f 402 28 in in IN cord-001257-t21l6i3f 402 29 vitro vitro FW cord-001257-t21l6i3f 402 30 replication replication NN cord-001257-t21l6i3f 402 31 assay assay NN cord-001257-t21l6i3f 402 32 containing contain VBG cord-001257-t21l6i3f 402 33 cellular cellular JJ cord-001257-t21l6i3f 402 34 AtRH5 atrh5 NN cord-001257-t21l6i3f 402 35 and and CC cord-001257-t21l6i3f 402 36 AtRH20 AtRH20 NNP cord-001257-t21l6i3f 402 37 helicases helicase NNS cord-001257-t21l6i3f 402 38 ( ( -LRB- cord-001257-t21l6i3f 402 39 lane lane NN cord-001257-t21l6i3f 402 40 2 2 CD cord-001257-t21l6i3f 402 41 in in IN cord-001257-t21l6i3f 402 42 panel panel NN cord-001257-t21l6i3f 402 43 B b NN cord-001257-t21l6i3f 402 44 ) ) -RRB- cord-001257-t21l6i3f 402 45 . . . cord-001257-t21l6i3f 403 1 The the DT cord-001257-t21l6i3f 403 2 blot blot NN cord-001257-t21l6i3f 403 3 contains contain VBZ cord-001257-t21l6i3f 403 4 the the DT cord-001257-t21l6i3f 403 5 same same JJ cord-001257-t21l6i3f 403 6 amount amount NN cord-001257-t21l6i3f 403 7 of of IN cord-001257-t21l6i3f 403 8 cold cold JJ cord-001257-t21l6i3f 403 9 ( ( -LRB- cord-001257-t21l6i3f 403 10 + + NN cord-001257-t21l6i3f 403 11 ) ) -RRB- cord-001257-t21l6i3f 403 12 and and CC cord-001257-t21l6i3f 403 13 ( ( -LRB- cord-001257-t21l6i3f 403 14 2)stranded 2)stranded NNP cord-001257-t21l6i3f 403 15 DI-72 DI-72 NNP cord-001257-t21l6i3f 403 16 RNA RNA NNP cord-001257-t21l6i3f 403 17 , , , cord-001257-t21l6i3f 403 18 while while IN cord-001257-t21l6i3f 403 19 the the DT cord-001257-t21l6i3f 403 20 32 32 CD cord-001257-t21l6i3f 403 21 P p NN cord-001257-t21l6i3f 403 22 - - HYPH cord-001257-t21l6i3f 403 23 labeled label VBN cord-001257-t21l6i3f 403 24 repRNA repRNA NNS cord-001257-t21l6i3f 403 25 probes probe NNS cord-001257-t21l6i3f 403 26 were be VBD cord-001257-t21l6i3f 403 27 generated generate VBN cord-001257-t21l6i3f 403 28 as as IN cord-001257-t21l6i3f 403 29 in in IN cord-001257-t21l6i3f 403 30 panel panel NN cord-001257-t21l6i3f 404 1 B. B. NNP cord-001257-t21l6i3f 404 2 The the DT cord-001257-t21l6i3f 404 3 ratio ratio NN cord-001257-t21l6i3f 404 4 of of IN cord-001257-t21l6i3f 404 5 ( ( -LRB- cord-001257-t21l6i3f 404 6 + + NN cord-001257-t21l6i3f 404 7 ) ) -RRB- cord-001257-t21l6i3f 404 8 and and CC cord-001257-t21l6i3f 404 9 ( ( -LRB- cord-001257-t21l6i3f 404 10 2)-stranded 2)-stranded CD cord-001257-t21l6i3f 404 11 RNA RNA NNP cord-001257-t21l6i3f 404 12 products product NNS cord-001257-t21l6i3f 404 13 was be VBD cord-001257-t21l6i3f 404 14 estimated estimate VBN cord-001257-t21l6i3f 404 15 . . . cord-001257-t21l6i3f 405 1 ( ( -LRB- cord-001257-t21l6i3f 405 2 PDF PDF NNP cord-001257-t21l6i3f 405 3 ) ) -RRB- cord-001257-t21l6i3f 405 4 Figure figure NN cord-001257-t21l6i3f 405 5 S4 S4 NNP cord-001257-t21l6i3f 405 6 Cell cell NN cord-001257-t21l6i3f 405 7 - - HYPH cord-001257-t21l6i3f 405 8 free free JJ cord-001257-t21l6i3f 405 9 TBSV TBSV NNP cord-001257-t21l6i3f 405 10 replication replication NN cord-001257-t21l6i3f 405 11 assay assay NN cord-001257-t21l6i3f 405 12 supports support VBZ cord-001257-t21l6i3f 405 13 a a DT cord-001257-t21l6i3f 405 14 role role NN cord-001257-t21l6i3f 405 15 for for IN cord-001257-t21l6i3f 405 16 Fal1p Fal1p NNP cord-001257-t21l6i3f 405 17 and and CC cord-001257-t21l6i3f 405 18 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 405 19 helicases helicase NNS cord-001257-t21l6i3f 405 20 in in IN cord-001257-t21l6i3f 405 21 plus plus JJ cord-001257-t21l6i3f 405 22 - - HYPH cord-001257-t21l6i3f 405 23 strand strand NN cord-001257-t21l6i3f 405 24 synthesis synthesis NN cord-001257-t21l6i3f 405 25 . . . cord-001257-t21l6i3f 406 1 ( ( -LRB- cord-001257-t21l6i3f 406 2 A a NN cord-001257-t21l6i3f 406 3 ) ) -RRB- cord-001257-t21l6i3f 406 4 Scheme scheme NN cord-001257-t21l6i3f 406 5 of of IN cord-001257-t21l6i3f 406 6 the the DT cord-001257-t21l6i3f 406 7 CFE CFE NNP cord-001257-t21l6i3f 406 8 - - HYPH cord-001257-t21l6i3f 406 9 based base VBN cord-001257-t21l6i3f 406 10 TBSV TBSV NNP cord-001257-t21l6i3f 406 11 replication replication NN cord-001257-t21l6i3f 406 12 assay assay NN cord-001257-t21l6i3f 406 13 . . . cord-001257-t21l6i3f 407 1 TBSV TBSV NNP cord-001257-t21l6i3f 407 2 DI-72 DI-72 NNP cord-001257-t21l6i3f 407 3 ( ( -LRB- cord-001257-t21l6i3f 407 4 + + NN cord-001257-t21l6i3f 407 5 ) ) -RRB- cord-001257-t21l6i3f 408 1 repRNA repRNA NNP cord-001257-t21l6i3f 408 2 was be VBD cord-001257-t21l6i3f 408 3 added add VBN cord-001257-t21l6i3f 408 4 to to IN cord-001257-t21l6i3f 408 5 the the DT cord-001257-t21l6i3f 408 6 whole whole JJ cord-001257-t21l6i3f 408 7 cell cell NN cord-001257-t21l6i3f 408 8 extract extract NN cord-001257-t21l6i3f 408 9 ( ( -LRB- cord-001257-t21l6i3f 408 10 CFE CFE NNP cord-001257-t21l6i3f 408 11 ) ) -RRB- cord-001257-t21l6i3f 408 12 prepared prepare VBD cord-001257-t21l6i3f 408 13 from from IN cord-001257-t21l6i3f 408 14 either either CC cord-001257-t21l6i3f 408 15 WT WT NNP cord-001257-t21l6i3f 408 16 yeast yeast NN cord-001257-t21l6i3f 408 17 or or CC cord-001257-t21l6i3f 408 18 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 408 19 - - HYPH cord-001257-t21l6i3f 408 20 depleted deplete VBN cord-001257-t21l6i3f 408 21 and and CC cord-001257-t21l6i3f 408 22 ts ts JJ cord-001257-t21l6i3f 408 23 - - HYPH cord-001257-t21l6i3f 408 24 Fal1p fal1p NN cord-001257-t21l6i3f 408 25 - - HYPH cord-001257-t21l6i3f 408 26 inactivated inactivate VBN cord-001257-t21l6i3f 408 27 yeast yeast NN cord-001257-t21l6i3f 408 28 strain strain NN cord-001257-t21l6i3f 408 29 expressing express VBG cord-001257-t21l6i3f 408 30 p33 p33 CD cord-001257-t21l6i3f 408 31 and and CC cord-001257-t21l6i3f 408 32 p92 p92 NN cord-001257-t21l6i3f 408 33 pol pol NN cord-001257-t21l6i3f 408 34 replication replication NN cord-001257-t21l6i3f 408 35 proteins protein NNS cord-001257-t21l6i3f 408 36 . . . cord-001257-t21l6i3f 409 1 The the DT cord-001257-t21l6i3f 409 2 membrane membrane NN cord-001257-t21l6i3f 409 3 and and CC cord-001257-t21l6i3f 409 4 soluble soluble JJ cord-001257-t21l6i3f 409 5 fractions fraction NNS cord-001257-t21l6i3f 409 6 were be VBD cord-001257-t21l6i3f 409 7 separated separate VBN cord-001257-t21l6i3f 409 8 at at IN cord-001257-t21l6i3f 409 9 the the DT cord-001257-t21l6i3f 409 10 end end NN cord-001257-t21l6i3f 409 11 of of IN cord-001257-t21l6i3f 409 12 the the DT cord-001257-t21l6i3f 409 13 replication replication NN cord-001257-t21l6i3f 409 14 assay assay NN cord-001257-t21l6i3f 409 15 by by IN cord-001257-t21l6i3f 409 16 centrifugation centrifugation NN cord-001257-t21l6i3f 409 17 . . . cord-001257-t21l6i3f 410 1 ( ( -LRB- cord-001257-t21l6i3f 410 2 B b NN cord-001257-t21l6i3f 410 3 ) ) -RRB- cord-001257-t21l6i3f 410 4 Detection detection NN cord-001257-t21l6i3f 410 5 of of IN cord-001257-t21l6i3f 410 6 single single JJ cord-001257-t21l6i3f 410 7 - - HYPH cord-001257-t21l6i3f 410 8 and and CC cord-001257-t21l6i3f 410 9 doublestranded doublestrande VBD cord-001257-t21l6i3f 410 10 RNA RNA NNP cord-001257-t21l6i3f 410 11 products product NNS cord-001257-t21l6i3f 410 12 produced produce VBN cord-001257-t21l6i3f 410 13 in in IN cord-001257-t21l6i3f 410 14 the the DT cord-001257-t21l6i3f 410 15 cell cell NN cord-001257-t21l6i3f 410 16 - - HYPH cord-001257-t21l6i3f 410 17 free free JJ cord-001257-t21l6i3f 410 18 TBSV TBSV NNP cord-001257-t21l6i3f 410 19 replication replication NN cord-001257-t21l6i3f 410 20 assays assay NNS cord-001257-t21l6i3f 410 21 . . . cord-001257-t21l6i3f 410 22 ' ' '' cord-001257-t21l6i3f 410 23 ' ' '' cord-001257-t21l6i3f 410 24 T T NNP cord-001257-t21l6i3f 410 25 ' ' '' cord-001257-t21l6i3f 410 26 ' ' '' cord-001257-t21l6i3f 410 27 total total NN cord-001257-t21l6i3f 410 28 , , , cord-001257-t21l6i3f 410 29 ' ' '' cord-001257-t21l6i3f 410 30 ' ' '' cord-001257-t21l6i3f 410 31 M M NNP cord-001257-t21l6i3f 410 32 ' ' '' cord-001257-t21l6i3f 410 33 ' ' '' cord-001257-t21l6i3f 410 34 membrane membrane NN cord-001257-t21l6i3f 410 35 fraction fraction NN cord-001257-t21l6i3f 410 36 , , , cord-001257-t21l6i3f 410 37 ' ' '' cord-001257-t21l6i3f 410 38 'S 's VBZ cord-001257-t21l6i3f 410 39 ' ' '' cord-001257-t21l6i3f 410 40 ' ' `` cord-001257-t21l6i3f 410 41 soluble soluble JJ cord-001257-t21l6i3f 410 42 fraction fraction NN cord-001257-t21l6i3f 410 43 . . . cord-001257-t21l6i3f 411 1 Note note VB cord-001257-t21l6i3f 411 2 that that IN cord-001257-t21l6i3f 411 3 the the DT cord-001257-t21l6i3f 411 4 ssRNA ssRNA `` cord-001257-t21l6i3f 411 5 present present JJ cord-001257-t21l6i3f 411 6 in in IN cord-001257-t21l6i3f 411 7 the the DT cord-001257-t21l6i3f 411 8 ' ' `` cord-001257-t21l6i3f 411 9 'S 's NN cord-001257-t21l6i3f 411 10 ' ' '' cord-001257-t21l6i3f 411 11 ' ' '' cord-001257-t21l6i3f 411 12 fraction fraction NN cord-001257-t21l6i3f 411 13 represents represent VBZ cord-001257-t21l6i3f 411 14 the the DT cord-001257-t21l6i3f 411 15 released release VBN cord-001257-t21l6i3f 411 16 ( ( -LRB- cord-001257-t21l6i3f 411 17 + + NNS cord-001257-t21l6i3f 411 18 ) ) -RRB- cord-001257-t21l6i3f 411 19 repRNA repRNA NNS cord-001257-t21l6i3f 411 20 products product NNS cord-001257-t21l6i3f 411 21 from from IN cord-001257-t21l6i3f 411 22 the the DT cord-001257-t21l6i3f 411 23 membrane membrane NN cord-001257-t21l6i3f 411 24 - - HYPH cord-001257-t21l6i3f 411 25 bound bind VBN cord-001257-t21l6i3f 411 26 VRCs vrcs NN cord-001257-t21l6i3f 411 27 . . . cord-001257-t21l6i3f 412 1 ( ( -LRB- cord-001257-t21l6i3f 412 2 C C NNP cord-001257-t21l6i3f 412 3 ) ) -RRB- cord-001257-t21l6i3f 412 4 Scheme Scheme NNP cord-001257-t21l6i3f 412 5 of of IN cord-001257-t21l6i3f 412 6 the the DT cord-001257-t21l6i3f 412 7 CFE CFE NNP cord-001257-t21l6i3f 412 8 - - HYPH cord-001257-t21l6i3f 412 9 based base VBN cord-001257-t21l6i3f 412 10 TBSV TBSV NNP cord-001257-t21l6i3f 412 11 replication replication NN cord-001257-t21l6i3f 412 12 assay assay NN cord-001257-t21l6i3f 412 13 with with IN cord-001257-t21l6i3f 412 14 purified purify VBN cord-001257-t21l6i3f 412 15 recombinant recombinant JJ cord-001257-t21l6i3f 412 16 cellular cellular JJ cord-001257-t21l6i3f 412 17 helicases helicase NNS cord-001257-t21l6i3f 412 18 . . . cord-001257-t21l6i3f 413 1 ( ( -LRB- cord-001257-t21l6i3f 413 2 D D NNP cord-001257-t21l6i3f 413 3 ) ) -RRB- cord-001257-t21l6i3f 413 4 Detection detection NN cord-001257-t21l6i3f 413 5 of of IN cord-001257-t21l6i3f 413 6 single single JJ cord-001257-t21l6i3f 413 7 - - HYPH cord-001257-t21l6i3f 413 8 and and CC cord-001257-t21l6i3f 413 9 double double RB cord-001257-t21l6i3f 413 10 - - HYPH cord-001257-t21l6i3f 413 11 stranded strand VBN cord-001257-t21l6i3f 413 12 RNA RNA NNP cord-001257-t21l6i3f 413 13 products product NNS cord-001257-t21l6i3f 413 14 produced produce VBN cord-001257-t21l6i3f 413 15 in in IN cord-001257-t21l6i3f 413 16 the the DT cord-001257-t21l6i3f 413 17 cell cell NN cord-001257-t21l6i3f 413 18 - - HYPH cord-001257-t21l6i3f 413 19 free free JJ cord-001257-t21l6i3f 413 20 TBSV TBSV NNP cord-001257-t21l6i3f 413 21 replication replication NN cord-001257-t21l6i3f 413 22 assays assay NNS cord-001257-t21l6i3f 413 23 by by IN cord-001257-t21l6i3f 413 24 denaturing denature VBG cord-001257-t21l6i3f 413 25 PAGE page NN cord-001257-t21l6i3f 413 26 analysis analysis NN cord-001257-t21l6i3f 413 27 of of IN cord-001257-t21l6i3f 413 28 the the DT cord-001257-t21l6i3f 413 29 32 32 CD cord-001257-t21l6i3f 413 30 P p NN cord-001257-t21l6i3f 413 31 - - HYPH cord-001257-t21l6i3f 413 32 labeled label VBN cord-001257-t21l6i3f 413 33 TBSV TBSV NNP cord-001257-t21l6i3f 413 34 repRNA repRNA NNS cord-001257-t21l6i3f 413 35 products product NNS cord-001257-t21l6i3f 413 36 ( ( -LRB- cord-001257-t21l6i3f 413 37 See see VB cord-001257-t21l6i3f 413 38 panel panel NN cord-001257-t21l6i3f 413 39 C c NN cord-001257-t21l6i3f 413 40 ) ) -RRB- cord-001257-t21l6i3f 413 41 . . . cord-001257-t21l6i3f 414 1 The the DT cord-001257-t21l6i3f 414 2 assay assay NN cord-001257-t21l6i3f 414 3 also also RB cord-001257-t21l6i3f 414 4 contained contain VBD cord-001257-t21l6i3f 414 5 purified purify VBN cord-001257-t21l6i3f 414 6 recombinant recombinant JJ cord-001257-t21l6i3f 414 7 AtRH2 atrh2 NN cord-001257-t21l6i3f 414 8 or or CC cord-001257-t21l6i3f 414 9 AtRH5 atrh5 NN cord-001257-t21l6i3f 414 10 ( ( -LRB- cord-001257-t21l6i3f 414 11 0.15 0.15 CD cord-001257-t21l6i3f 414 12 mg mg NNP cord-001257-t21l6i3f 414 13 ) ) -RRB- cord-001257-t21l6i3f 414 14 or or CC cord-001257-t21l6i3f 414 15 MBP MBP NNP cord-001257-t21l6i3f 414 16 ( ( -LRB- cord-001257-t21l6i3f 414 17 the the DT cord-001257-t21l6i3f 414 18 same same JJ cord-001257-t21l6i3f 414 19 molar molar JJ cord-001257-t21l6i3f 414 20 amount amount NN cord-001257-t21l6i3f 414 21 as as IN cord-001257-t21l6i3f 414 22 the the DT cord-001257-t21l6i3f 414 23 helicases helicase NNS cord-001257-t21l6i3f 414 24 ) ) -RRB- cord-001257-t21l6i3f 414 25 as as IN cord-001257-t21l6i3f 414 26 a a DT cord-001257-t21l6i3f 414 27 control control NN cord-001257-t21l6i3f 414 28 . . . cord-001257-t21l6i3f 415 1 Odd odd JJ cord-001257-t21l6i3f 415 2 numbered number VBD cord-001257-t21l6i3f 415 3 lanes lane NNS cord-001257-t21l6i3f 415 4 represent represent VBP cord-001257-t21l6i3f 415 5 replicase replicase NN cord-001257-t21l6i3f 415 6 products product NNS cord-001257-t21l6i3f 415 7 , , , cord-001257-t21l6i3f 415 8 which which WDT cord-001257-t21l6i3f 415 9 were be VBD cord-001257-t21l6i3f 415 10 not not RB cord-001257-t21l6i3f 415 11 heat heat NN cord-001257-t21l6i3f 415 12 treated treat VBN cord-001257-t21l6i3f 415 13 ( ( -LRB- cord-001257-t21l6i3f 415 14 thus thus RB cord-001257-t21l6i3f 415 15 both both DT cord-001257-t21l6i3f 415 16 ssRNA ssrna NN cord-001257-t21l6i3f 415 17 and and CC cord-001257-t21l6i3f 415 18 dsRNA dsrna NN cord-001257-t21l6i3f 415 19 products product NNS cord-001257-t21l6i3f 415 20 are be VBP cord-001257-t21l6i3f 415 21 present present JJ cord-001257-t21l6i3f 415 22 ) ) -RRB- cord-001257-t21l6i3f 415 23 , , , cord-001257-t21l6i3f 415 24 while while IN cord-001257-t21l6i3f 415 25 the the DT cord-001257-t21l6i3f 415 26 even even RB cord-001257-t21l6i3f 415 27 numbered number VBD cord-001257-t21l6i3f 415 28 lanes lane NNS cord-001257-t21l6i3f 415 29 show show VBP cord-001257-t21l6i3f 415 30 the the DT cord-001257-t21l6i3f 415 31 heat heat NN cord-001257-t21l6i3f 415 32 - - HYPH cord-001257-t21l6i3f 415 33 treated treat VBN cord-001257-t21l6i3f 415 34 replicase replicase NN cord-001257-t21l6i3f 415 35 products product NNS cord-001257-t21l6i3f 415 36 ( ( -LRB- cord-001257-t21l6i3f 415 37 only only RB cord-001257-t21l6i3f 415 38 ssRNA ssrna NN cord-001257-t21l6i3f 415 39 is be VBZ cord-001257-t21l6i3f 415 40 present present JJ cord-001257-t21l6i3f 415 41 ) ) -RRB- cord-001257-t21l6i3f 415 42 . . . cord-001257-t21l6i3f 416 1 The the DT cord-001257-t21l6i3f 416 2 % % NN cord-001257-t21l6i3f 416 3 of of IN cord-001257-t21l6i3f 416 4 dsRNA dsrna NN cord-001257-t21l6i3f 416 5 and and CC cord-001257-t21l6i3f 416 6 ssRNA ssRNA . cord-001257-t21l6i3f 417 1 in in IN cord-001257-t21l6i3f 417 2 the the DT cord-001257-t21l6i3f 417 3 samples sample NNS cord-001257-t21l6i3f 417 4 are be VBP cord-001257-t21l6i3f 417 5 shown show VBN cord-001257-t21l6i3f 417 6 . . . cord-001257-t21l6i3f 418 1 Note note VB cord-001257-t21l6i3f 418 2 that that IN cord-001257-t21l6i3f 418 3 , , , cord-001257-t21l6i3f 418 4 in in IN cord-001257-t21l6i3f 418 5 the the DT cord-001257-t21l6i3f 418 6 nondenatured nondenatured JJ cord-001257-t21l6i3f 418 7 samples sample NNS cord-001257-t21l6i3f 418 8 , , , cord-001257-t21l6i3f 418 9 the the DT cord-001257-t21l6i3f 418 10 dsRNA dsrna NN cord-001257-t21l6i3f 418 11 product product NN cord-001257-t21l6i3f 418 12 represents represent VBZ cord-001257-t21l6i3f 418 13 the the DT cord-001257-t21l6i3f 418 14 annealed anneal VBN cord-001257-t21l6i3f 418 15 ( ( -LRB- cord-001257-t21l6i3f 418 16 2)RNA 2)rna CD cord-001257-t21l6i3f 418 17 and and CC cord-001257-t21l6i3f 418 18 the the DT cord-001257-t21l6i3f 418 19 ( ( -LRB- cord-001257-t21l6i3f 418 20 + + CD cord-001257-t21l6i3f 418 21 ) ) -RRB- cord-001257-t21l6i3f 418 22 RNA RNA NNP cord-001257-t21l6i3f 418 23 , , , cord-001257-t21l6i3f 418 24 while while IN cord-001257-t21l6i3f 418 25 the the DT cord-001257-t21l6i3f 418 26 ssRNA ssRNA NNP cord-001257-t21l6i3f 418 27 products product NNS cord-001257-t21l6i3f 418 28 represents represent VBZ cord-001257-t21l6i3f 418 29 the the DT cord-001257-t21l6i3f 418 30 newly newly RB cord-001257-t21l6i3f 418 31 made make VBN cord-001257-t21l6i3f 418 32 ( ( -LRB- cord-001257-t21l6i3f 418 33 + + CC cord-001257-t21l6i3f 418 34 ) ) -RRB- cord-001257-t21l6i3f 418 35 RNA RNA NNP cord-001257-t21l6i3f 418 36 products product NNS cord-001257-t21l6i3f 418 37 . . . cord-001257-t21l6i3f 419 1 Each each DT cord-001257-t21l6i3f 419 2 experiment experiment NN cord-001257-t21l6i3f 419 3 was be VBD cord-001257-t21l6i3f 419 4 repeated repeat VBN cord-001257-t21l6i3f 419 5 three three CD cord-001257-t21l6i3f 419 6 times time NNS cord-001257-t21l6i3f 419 7 . . . cord-001257-t21l6i3f 420 1 ( ( -LRB- cord-001257-t21l6i3f 420 2 PDF PDF NNP cord-001257-t21l6i3f 420 3 ) ) -RRB- cord-001257-t21l6i3f 420 4 Figure figure NN cord-001257-t21l6i3f 420 5 S5 S5 NNP cord-001257-t21l6i3f 420 6 AtRH5 AtRH5 NNP cord-001257-t21l6i3f 420 7 is be VBZ cord-001257-t21l6i3f 420 8 a a DT cord-001257-t21l6i3f 420 9 component component NN cord-001257-t21l6i3f 420 10 of of IN cord-001257-t21l6i3f 420 11 the the DT cord-001257-t21l6i3f 420 12 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 420 13 replicase replicase NN cord-001257-t21l6i3f 420 14 in in IN cord-001257-t21l6i3f 420 15 yeast yeast NN cord-001257-t21l6i3f 420 16 . . . cord-001257-t21l6i3f 421 1 ( ( -LRB- cord-001257-t21l6i3f 421 2 A a NN cord-001257-t21l6i3f 421 3 ) ) -RRB- cord-001257-t21l6i3f 422 1 The the DT cord-001257-t21l6i3f 422 2 membrane membrane NN cord-001257-t21l6i3f 422 3 - - HYPH cord-001257-t21l6i3f 422 4 bound bind VBN cord-001257-t21l6i3f 422 5 tombusvirus tombusvirus NN cord-001257-t21l6i3f 422 6 replicase replicase NNP cord-001257-t21l6i3f 422 7 was be VBD cord-001257-t21l6i3f 422 8 purified purify VBN cord-001257-t21l6i3f 422 9 via via IN cord-001257-t21l6i3f 422 10 solubilization solubilization NN cord-001257-t21l6i3f 422 11 of of IN cord-001257-t21l6i3f 422 12 the the DT cord-001257-t21l6i3f 422 13 FLAG flag NN cord-001257-t21l6i3f 422 14 - - HYPH cord-001257-t21l6i3f 422 15 tagged tag VBN cord-001257-t21l6i3f 422 16 p33F p33f NN cord-001257-t21l6i3f 422 17 from from IN cord-001257-t21l6i3f 422 18 yeast yeast NN cord-001257-t21l6i3f 422 19 extracts extract NNS cord-001257-t21l6i3f 422 20 using use VBG cord-001257-t21l6i3f 422 21 a a DT cord-001257-t21l6i3f 422 22 FLAG flag NN cord-001257-t21l6i3f 422 23 - - HYPH cord-001257-t21l6i3f 422 24 affinity affinity NN cord-001257-t21l6i3f 422 25 column column NN cord-001257-t21l6i3f 422 26 ( ( -LRB- cord-001257-t21l6i3f 422 27 lanes lane NNS cord-001257-t21l6i3f 422 28 1 1 CD cord-001257-t21l6i3f 422 29 - - SYM cord-001257-t21l6i3f 422 30 2 2 CD cord-001257-t21l6i3f 422 31 ) ) -RRB- cord-001257-t21l6i3f 422 32 . . . cord-001257-t21l6i3f 423 1 Yeast yeast NN cord-001257-t21l6i3f 423 2 not not RB cord-001257-t21l6i3f 423 3 expressing express VBG cord-001257-t21l6i3f 423 4 p33F p33f NN cord-001257-t21l6i3f 423 5 was be VBD cord-001257-t21l6i3f 423 6 used use VBN cord-001257-t21l6i3f 423 7 as as IN cord-001257-t21l6i3f 423 8 a a DT cord-001257-t21l6i3f 423 9 control control NN cord-001257-t21l6i3f 423 10 ( ( -LRB- cord-001257-t21l6i3f 423 11 lane lane NN cord-001257-t21l6i3f 423 12 3 3 CD cord-001257-t21l6i3f 423 13 ) ) -RRB- cord-001257-t21l6i3f 423 14 . . . cord-001257-t21l6i3f 424 1 Top top JJ cord-001257-t21l6i3f 424 2 panel panel NN cord-001257-t21l6i3f 424 3 : : : cord-001257-t21l6i3f 424 4 Western western JJ cord-001257-t21l6i3f 424 5 blot blot NN cord-001257-t21l6i3f 424 6 analysis analysis NN cord-001257-t21l6i3f 424 7 of of IN cord-001257-t21l6i3f 424 8 FLAG flag NN cord-001257-t21l6i3f 424 9 - - HYPH cord-001257-t21l6i3f 424 10 tagged tag VBN cord-001257-t21l6i3f 424 11 p33F p33f NN cord-001257-t21l6i3f 424 12 with with IN cord-001257-t21l6i3f 424 13 anti anti JJ cord-001257-t21l6i3f 424 14 - - JJ cord-001257-t21l6i3f 424 15 FLAG flag JJ cord-001257-t21l6i3f 424 16 antibody antibody NN cord-001257-t21l6i3f 424 17 . . . cord-001257-t21l6i3f 425 1 Bottom bottom JJ cord-001257-t21l6i3f 425 2 panel panel NN cord-001257-t21l6i3f 425 3 : : : cord-001257-t21l6i3f 425 4 Western western JJ cord-001257-t21l6i3f 425 5 blot blot NN cord-001257-t21l6i3f 425 6 analysis analysis NN cord-001257-t21l6i3f 425 7 of of IN cord-001257-t21l6i3f 425 8 His -PRON- PRP$ cord-001257-t21l6i3f 425 9 6 6 CD cord-001257-t21l6i3f 425 10 -tagged -tagged CD cord-001257-t21l6i3f 425 11 AtRH5 atrh5 NN cord-001257-t21l6i3f 425 12 with with IN cord-001257-t21l6i3f 425 13 anti anti JJ cord-001257-t21l6i3f 425 14 - - JJ cord-001257-t21l6i3f 425 15 His -PRON- PRP$ cord-001257-t21l6i3f 425 16 6 6 CD cord-001257-t21l6i3f 425 17 antibody antibody NN cord-001257-t21l6i3f 425 18 in in IN cord-001257-t21l6i3f 425 19 the the DT cord-001257-t21l6i3f 425 20 affinity affinity NN cord-001257-t21l6i3f 425 21 - - HYPH cord-001257-t21l6i3f 425 22 purified purify VBN cord-001257-t21l6i3f 425 23 replicase replicase NN cord-001257-t21l6i3f 425 24 preparations preparation NNS cord-001257-t21l6i3f 425 25 . . . cord-001257-t21l6i3f 426 1 Note note VB cord-001257-t21l6i3f 426 2 that that IN cord-001257-t21l6i3f 426 3 ' ' `` cord-001257-t21l6i3f 426 4 ' ' `` cord-001257-t21l6i3f 426 5 soluble soluble JJ cord-001257-t21l6i3f 426 6 ' ' '' cord-001257-t21l6i3f 426 7 ' ' '' cord-001257-t21l6i3f 426 8 represents represent VBZ cord-001257-t21l6i3f 426 9 the the DT cord-001257-t21l6i3f 426 10 total total JJ cord-001257-t21l6i3f 426 11 protein protein NN cord-001257-t21l6i3f 426 12 extract extract NN cord-001257-t21l6i3f 426 13 from from IN cord-001257-t21l6i3f 426 14 yeast yeast NN cord-001257-t21l6i3f 426 15 demonstrating demonstrate VBG cord-001257-t21l6i3f 426 16 comparable comparable JJ cord-001257-t21l6i3f 426 17 levels level NNS cord-001257-t21l6i3f 426 18 of of IN cord-001257-t21l6i3f 426 19 His -PRON- PRP$ cord-001257-t21l6i3f 426 20 6 6 CD cord-001257-t21l6i3f 426 21 -AtRH5 -atrh5 NN cord-001257-t21l6i3f 426 22 in in IN cord-001257-t21l6i3f 426 23 each each DT cord-001257-t21l6i3f 426 24 sample sample NN cord-001257-t21l6i3f 426 25 ( ( -LRB- cord-001257-t21l6i3f 426 26 lanes lane NNS cord-001257-t21l6i3f 426 27 4 4 CD cord-001257-t21l6i3f 426 28 - - SYM cord-001257-t21l6i3f 426 29 6 6 CD cord-001257-t21l6i3f 426 30 ) ) -RRB- cord-001257-t21l6i3f 426 31 . . . cord-001257-t21l6i3f 427 1 Each each DT cord-001257-t21l6i3f 427 2 experiment experiment NN cord-001257-t21l6i3f 427 3 was be VBD cord-001257-t21l6i3f 427 4 repeated repeat VBN cord-001257-t21l6i3f 427 5 three three CD cord-001257-t21l6i3f 427 6 times time NNS cord-001257-t21l6i3f 427 7 . . . cord-001257-t21l6i3f 428 1 ( ( -LRB- cord-001257-t21l6i3f 428 2 B b NN cord-001257-t21l6i3f 428 3 ) ) -RRB- cord-001257-t21l6i3f 428 4 Interaction interaction NN cord-001257-t21l6i3f 428 5 between between IN cord-001257-t21l6i3f 428 6 yeast yeast NN cord-001257-t21l6i3f 428 7 DEAD DEAD NNP cord-001257-t21l6i3f 428 8 - - HYPH cord-001257-t21l6i3f 428 9 box box NN cord-001257-t21l6i3f 428 10 helicases helicase NNS cord-001257-t21l6i3f 428 11 and and CC cord-001257-t21l6i3f 428 12 the the DT cord-001257-t21l6i3f 428 13 TBSV TBSV NNP cord-001257-t21l6i3f 428 14 p33 p33 CD cord-001257-t21l6i3f 428 15 replication replication NN cord-001257-t21l6i3f 428 16 protein protein NN cord-001257-t21l6i3f 428 17 based base VBN cord-001257-t21l6i3f 428 18 on on IN cord-001257-t21l6i3f 428 19 the the DT cord-001257-t21l6i3f 428 20 membrane membrane NN cord-001257-t21l6i3f 428 21 yeast yeast NN cord-001257-t21l6i3f 428 22 two two CD cord-001257-t21l6i3f 428 23 hybrid hybrid JJ cord-001257-t21l6i3f 428 24 assay assay NN cord-001257-t21l6i3f 428 25 ( ( -LRB- cord-001257-t21l6i3f 428 26 split split VB cord-001257-t21l6i3f 428 27 - - HYPH cord-001257-t21l6i3f 428 28 ubiquitin ubiquitin NN cord-001257-t21l6i3f 428 29 assay assay NN cord-001257-t21l6i3f 428 30 ) ) -RRB- cord-001257-t21l6i3f 428 31 . . . cord-001257-t21l6i3f 429 1 The the DT cord-001257-t21l6i3f 429 2 bait bait NN cord-001257-t21l6i3f 429 3 p33 p33 NN cord-001257-t21l6i3f 429 4 was be VBD cord-001257-t21l6i3f 429 5 co co VBN cord-001257-t21l6i3f 429 6 - - VBN cord-001257-t21l6i3f 429 7 expressed express VBN cord-001257-t21l6i3f 429 8 with with IN cord-001257-t21l6i3f 429 9 the the DT cord-001257-t21l6i3f 429 10 prey prey NN cord-001257-t21l6i3f 429 11 full full JJ cord-001257-t21l6i3f 429 12 - - HYPH cord-001257-t21l6i3f 429 13 length length NN cord-001257-t21l6i3f 429 14 host host NN cord-001257-t21l6i3f 429 15 proteins protein NNS cord-001257-t21l6i3f 429 16 in in IN cord-001257-t21l6i3f 429 17 yeast yeast NN cord-001257-t21l6i3f 429 18 . . . cord-001257-t21l6i3f 430 1 The the DT cord-001257-t21l6i3f 430 2 yeast yeast NN cord-001257-t21l6i3f 430 3 Ssa1p ssa1p NN cord-001257-t21l6i3f 430 4 ( ( -LRB- cord-001257-t21l6i3f 430 5 HSP70 HSP70 NNP cord-001257-t21l6i3f 430 6 chaperone chaperone NN cord-001257-t21l6i3f 430 7 ) ) -RRB- cord-001257-t21l6i3f 430 8 , , , cord-001257-t21l6i3f 430 9 and and CC cord-001257-t21l6i3f 430 10 the the DT cord-001257-t21l6i3f 430 11 empty empty JJ cord-001257-t21l6i3f 430 12 prey prey NN cord-001257-t21l6i3f 430 13 vector vector NN cord-001257-t21l6i3f 430 14 ( ( -LRB- cord-001257-t21l6i3f 430 15 NubG NubG NNP cord-001257-t21l6i3f 430 16 ) ) -RRB- cord-001257-t21l6i3f 430 17 were be VBD cord-001257-t21l6i3f 430 18 used use VBN cord-001257-t21l6i3f 430 19 as as IN cord-001257-t21l6i3f 430 20 positive positive JJ cord-001257-t21l6i3f 430 21 and and CC cord-001257-t21l6i3f 430 22 negative negative JJ cord-001257-t21l6i3f 430 23 controls control NNS cord-001257-t21l6i3f 430 24 , , , cord-001257-t21l6i3f 430 25 respectively respectively RB cord-001257-t21l6i3f 430 26 . . . cord-001257-t21l6i3f 431 1 The the DT cord-001257-t21l6i3f 431 2 image image NN cord-001257-t21l6i3f 431 3 shows show VBZ cord-001257-t21l6i3f 431 4 10fold 10fold CD cord-001257-t21l6i3f 431 5 serial serial JJ cord-001257-t21l6i3f 431 6 dilutions dilution NNS cord-001257-t21l6i3f 431 7 of of IN cord-001257-t21l6i3f 431 8 yeast yeast NN cord-001257-t21l6i3f 431 9 cultures culture NNS cord-001257-t21l6i3f 431 10 . . . cord-001257-t21l6i3f 432 1 ( ( -LRB- cord-001257-t21l6i3f 432 2 PDF PDF NNP cord-001257-t21l6i3f 432 3 ) ) -RRB- cord-001257-t21l6i3f 432 4 Figure figure NN cord-001257-t21l6i3f 432 5 S6 S6 NNP cord-001257-t21l6i3f 432 6 Interaction Interaction NNP cord-001257-t21l6i3f 432 7 between between IN cord-001257-t21l6i3f 432 8 AtRH2 AtRH2 NNP cord-001257-t21l6i3f 432 9 and and CC cord-001257-t21l6i3f 432 10 AtRH5 AtRH5 NNP cord-001257-t21l6i3f 432 11 and and CC cord-001257-t21l6i3f 432 12 the the DT cord-001257-t21l6i3f 432 13 TBSV TBSV NNP cord-001257-t21l6i3f 432 14 p33 p33 CD cord-001257-t21l6i3f 432 15 replication replication NN cord-001257-t21l6i3f 432 16 protein protein NN cord-001257-t21l6i3f 432 17 . . . cord-001257-t21l6i3f 433 1 ( ( -LRB- cord-001257-t21l6i3f 433 2 A a NN cord-001257-t21l6i3f 433 3 ) ) -RRB- cord-001257-t21l6i3f 434 1 A a DT cord-001257-t21l6i3f 434 2 schematic schematic JJ cord-001257-t21l6i3f 434 3 representation representation NN cord-001257-t21l6i3f 434 4 of of IN cord-001257-t21l6i3f 434 5 viral viral JJ cord-001257-t21l6i3f 434 6 p33 p33 NN cord-001257-t21l6i3f 434 7 and and CC cord-001257-t21l6i3f 434 8 its -PRON- PRP$ cord-001257-t21l6i3f 434 9 derivatives derivative NNS cord-001257-t21l6i3f 434 10 used use VBN cord-001257-t21l6i3f 434 11 in in IN cord-001257-t21l6i3f 434 12 the the DT cord-001257-t21l6i3f 434 13 binding bind VBG cord-001257-t21l6i3f 434 14 assay assay NN cord-001257-t21l6i3f 434 15 ( ( -LRB- cord-001257-t21l6i3f 434 16 each each DT cord-001257-t21l6i3f 434 17 MBPtagged mbptagge VBN cord-001257-t21l6i3f 434 18 at at IN cord-001257-t21l6i3f 434 19 the the DT cord-001257-t21l6i3f 434 20 N n CD cord-001257-t21l6i3f 434 21 - - HYPH cord-001257-t21l6i3f 434 22 terminus terminus NN cord-001257-t21l6i3f 434 23 ) ) -RRB- cord-001257-t21l6i3f 434 24 . . . cord-001257-t21l6i3f 435 1 The the DT cord-001257-t21l6i3f 435 2 various various JJ cord-001257-t21l6i3f 435 3 domains domain NNS cord-001257-t21l6i3f 435 4 include include VBP cord-001257-t21l6i3f 435 5 : : : cord-001257-t21l6i3f 435 6 TMD TMD NNP cord-001257-t21l6i3f 435 7 , , , cord-001257-t21l6i3f 435 8 transmembrane transmembrane NN cord-001257-t21l6i3f 435 9 domain domain NN cord-001257-t21l6i3f 435 10 ; ; : cord-001257-t21l6i3f 435 11 RPR RPR NNP cord-001257-t21l6i3f 435 12 , , , cord-001257-t21l6i3f 435 13 arginine arginine NN cord-001257-t21l6i3f 435 14 - - HYPH cord-001257-t21l6i3f 435 15 proline proline NN cord-001257-t21l6i3f 435 16 - - HYPH cord-001257-t21l6i3f 435 17 rich rich JJ cord-001257-t21l6i3f 435 18 RNA RNA NNP cord-001257-t21l6i3f 435 19 binding binding NN cord-001257-t21l6i3f 435 20 domain domain NN cord-001257-t21l6i3f 435 21 ; ; : cord-001257-t21l6i3f 435 22 P p NN cord-001257-t21l6i3f 435 23 ; ; , cord-001257-t21l6i3f 435 24 phosphorylated phosphorylated JJ cord-001257-t21l6i3f 435 25 serine serine NN cord-001257-t21l6i3f 435 26 and and CC cord-001257-t21l6i3f 435 27 threonine threonine NN cord-001257-t21l6i3f 435 28 ; ; : cord-001257-t21l6i3f 435 29 S1 s1 NN cord-001257-t21l6i3f 435 30 and and CC cord-001257-t21l6i3f 435 31 S2 s2 NN cord-001257-t21l6i3f 435 32 subdomains subdomain NNS cord-001257-t21l6i3f 435 33 involved involve VBN cord-001257-t21l6i3f 435 34 in in IN cord-001257-t21l6i3f 435 35 p33 p33 CD cord-001257-t21l6i3f 435 36 : : : cord-001257-t21l6i3f 435 37 p33 p33 CD cord-001257-t21l6i3f 435 38 / / SYM cord-001257-t21l6i3f 435 39 p92 p92 CD cord-001257-t21l6i3f 435 40 interaction interaction NN cord-001257-t21l6i3f 435 41 . . . cord-001257-t21l6i3f 436 1 The the DT cord-001257-t21l6i3f 436 2 results result NNS cord-001257-t21l6i3f 436 3 of of IN cord-001257-t21l6i3f 436 4 the the DT cord-001257-t21l6i3f 436 5 in in FW cord-001257-t21l6i3f 436 6 vitro vitro FW cord-001257-t21l6i3f 436 7 binding bind VBG cord-001257-t21l6i3f 436 8 experiments experiment NNS cord-001257-t21l6i3f 436 9 are be VBP cord-001257-t21l6i3f 436 10 summarized summarize VBN cord-001257-t21l6i3f 436 11 ( ( -LRB- cord-001257-t21l6i3f 436 12 ' ' `` cord-001257-t21l6i3f 436 13 ' ' '' cord-001257-t21l6i3f 436 14 + + CC cord-001257-t21l6i3f 436 15 ' ' '' cord-001257-t21l6i3f 436 16 ' ' '' cord-001257-t21l6i3f 436 17 or or CC cord-001257-t21l6i3f 436 18 ' ' `` cord-001257-t21l6i3f 436 19 ' ' `` cord-001257-t21l6i3f 436 20 2 2 CD cord-001257-t21l6i3f 436 21 ' ' '' cord-001257-t21l6i3f 436 22 ' ' '' cord-001257-t21l6i3f 436 23 , , , cord-001257-t21l6i3f 436 24 based base VBN cord-001257-t21l6i3f 436 25 on on IN cord-001257-t21l6i3f 436 26 two two CD cord-001257-t21l6i3f 436 27 repeats repeat NNS cord-001257-t21l6i3f 436 28 ) ) -RRB- cord-001257-t21l6i3f 436 29 . . . cord-001257-t21l6i3f 437 1 ( ( -LRB- cord-001257-t21l6i3f 437 2 B b NN cord-001257-t21l6i3f 437 3 ) ) -RRB- cord-001257-t21l6i3f 438 1 In in IN cord-001257-t21l6i3f 438 2 vitro vitro FW cord-001257-t21l6i3f 438 3 pull pull VB cord-001257-t21l6i3f 438 4 - - HYPH cord-001257-t21l6i3f 438 5 down down RP cord-001257-t21l6i3f 438 6 assay assay NN cord-001257-t21l6i3f 438 7 of of IN cord-001257-t21l6i3f 438 8 His -PRON- PRP$ cord-001257-t21l6i3f 438 9 6 6 CD cord-001257-t21l6i3f 438 10 -tagged -tagged CD cord-001257-t21l6i3f 438 11 AtRH5 atrh5 NN cord-001257-t21l6i3f 438 12 ( ( -LRB- cord-001257-t21l6i3f 438 13 lanes lane NNS cord-001257-t21l6i3f 438 14 1 1 CD cord-001257-t21l6i3f 438 15 - - SYM cord-001257-t21l6i3f 438 16 4 4 CD cord-001257-t21l6i3f 438 17 ) ) -RRB- cord-001257-t21l6i3f 438 18 , , , cord-001257-t21l6i3f 438 19 His -PRON- PRP$ cord-001257-t21l6i3f 438 20 6 6 CD cord-001257-t21l6i3f 438 21 -peptide -peptide NN cord-001257-t21l6i3f 438 22 ( ( -LRB- cord-001257-t21l6i3f 438 23 lanes lane NNS cord-001257-t21l6i3f 438 24 5 5 CD cord-001257-t21l6i3f 438 25 - - SYM cord-001257-t21l6i3f 438 26 8 8 CD cord-001257-t21l6i3f 438 27 ) ) -RRB- cord-001257-t21l6i3f 438 28 or or CC cord-001257-t21l6i3f 438 29 His -PRON- PRP$ cord-001257-t21l6i3f 438 30 6 6 CD cord-001257-t21l6i3f 438 31 -tagged -tagged JJ cord-001257-t21l6i3f 438 32 AtRH2 atrh2 NN cord-001257-t21l6i3f 438 33 ( ( -LRB- cord-001257-t21l6i3f 438 34 lanes lane NNS cord-001257-t21l6i3f 438 35 [ [ -LRB- cord-001257-t21l6i3f 438 36 11 11 CD cord-001257-t21l6i3f 438 37 ] ] -RRB- cord-001257-t21l6i3f 438 38 [ [ -LRB- cord-001257-t21l6i3f 438 39 12 12 CD cord-001257-t21l6i3f 438 40 ] ] -RRB- cord-001257-t21l6i3f 438 41 [ [ -LRB- cord-001257-t21l6i3f 438 42 13 13 CD cord-001257-t21l6i3f 438 43 ] ] -RRB- cord-001257-t21l6i3f 438 44 [ [ -LRB- cord-001257-t21l6i3f 438 45 14 14 CD cord-001257-t21l6i3f 438 46 ] ] -RRB- cord-001257-t21l6i3f 438 47 with with IN cord-001257-t21l6i3f 438 48 MBP MBP NNP cord-001257-t21l6i3f 438 49 - - HYPH cord-001257-t21l6i3f 438 50 p33 p33 CD cord-001257-t21l6i3f 438 51 derivatives derivative NNS cord-001257-t21l6i3f 438 52 using use VBG cord-001257-t21l6i3f 438 53 amylose amylose JJ cord-001257-t21l6i3f 438 54 resin resin NN cord-001257-t21l6i3f 438 55 . . . cord-001257-t21l6i3f 439 1 Top top JJ cord-001257-t21l6i3f 439 2 panel panel NN cord-001257-t21l6i3f 439 3 : : : cord-001257-t21l6i3f 440 1 Western western JJ cord-001257-t21l6i3f 440 2 blot blot NN cord-001257-t21l6i3f 440 3 analysis analysis NN cord-001257-t21l6i3f 440 4 with with IN cord-001257-t21l6i3f 441 1 anti anti JJ cord-001257-t21l6i3f 441 2 - - JJ cord-001257-t21l6i3f 441 3 His -PRON- PRP$ cord-001257-t21l6i3f 441 4 antibody antibody NN cord-001257-t21l6i3f 441 5 of of IN cord-001257-t21l6i3f 441 6 His -PRON- PRP$ cord-001257-t21l6i3f 441 7 6 6 CD cord-001257-t21l6i3f 441 8 -tagged -tagged CD cord-001257-t21l6i3f 441 9 helicases helicase NNS cord-001257-t21l6i3f 441 10 pulled pull VBD cord-001257-t21l6i3f 441 11 down down RP cord-001257-t21l6i3f 441 12 with with IN cord-001257-t21l6i3f 441 13 MBP MBP NNP cord-001257-t21l6i3f 441 14 - - HYPH cord-001257-t21l6i3f 441 15 p33 p33 CD cord-001257-t21l6i3f 441 16 derivatives derivative NNS cord-001257-t21l6i3f 441 17 . . . cord-001257-t21l6i3f 442 1 Lane lane NN cord-001257-t21l6i3f 442 2 9 9 CD cord-001257-t21l6i3f 442 3 contains contain VBZ cord-001257-t21l6i3f 442 4 purified purify VBN cord-001257-t21l6i3f 442 5 His -PRON- PRP$ cord-001257-t21l6i3f 442 6 6 6 CD cord-001257-t21l6i3f 442 7 -tagged -tagged CD cord-001257-t21l6i3f 442 8 AtRH5 atrh5 NN cord-001257-t21l6i3f 442 9 as as IN cord-001257-t21l6i3f 442 10 a a DT cord-001257-t21l6i3f 442 11 standard standard NN cord-001257-t21l6i3f 442 12 . . . cord-001257-t21l6i3f 442 13 _SP cord-001257-t21l6i3f 443 1 Yeast yeast NN cord-001257-t21l6i3f 443 2 as as IN cord-001257-t21l6i3f 443 3 a a DT cord-001257-t21l6i3f 443 4 model model NN cord-001257-t21l6i3f 443 5 host host NN cord-001257-t21l6i3f 443 6 to to TO cord-001257-t21l6i3f 443 7 explore explore VB cord-001257-t21l6i3f 443 8 plant plant NN cord-001257-t21l6i3f 443 9 virus virus NN cord-001257-t21l6i3f 443 10 - - HYPH cord-001257-t21l6i3f 443 11 host host NN cord-001257-t21l6i3f 443 12 interactions interaction NNS cord-001257-t21l6i3f 444 1 Cytoplasmic cytoplasmic VB cord-001257-t21l6i3f 444 2 viral viral JJ cord-001257-t21l6i3f 444 3 replication replication NN cord-001257-t21l6i3f 444 4 complexes complex NNS cord-001257-t21l6i3f 445 1 The the DT cord-001257-t21l6i3f 445 2 dependence dependence NN cord-001257-t21l6i3f 445 3 of of IN cord-001257-t21l6i3f 445 4 viral viral JJ cord-001257-t21l6i3f 445 5 RNA RNA NNP cord-001257-t21l6i3f 445 6 replication replication NN cord-001257-t21l6i3f 445 7 on on IN cord-001257-t21l6i3f 445 8 coopted coopte VBN cord-001257-t21l6i3f 445 9 host host NN cord-001257-t21l6i3f 445 10 factors factor NNS cord-001257-t21l6i3f 445 11 + + CD cord-001257-t21l6i3f 445 12 ) ) -RRB- cord-001257-t21l6i3f 446 1 RNA RNA NNP cord-001257-t21l6i3f 446 2 viruses virus NNS cord-001257-t21l6i3f 446 3 rewire rewire VBP cord-001257-t21l6i3f 446 4 cellular cellular JJ cord-001257-t21l6i3f 446 5 pathways pathway NNS cord-001257-t21l6i3f 446 6 to to TO cord-001257-t21l6i3f 446 7 build build VB cord-001257-t21l6i3f 446 8 replication replication NN cord-001257-t21l6i3f 446 9 organelles organelle NNS cord-001257-t21l6i3f 446 10 Yeast yeast NN cord-001257-t21l6i3f 446 11 as as IN cord-001257-t21l6i3f 446 12 a a DT cord-001257-t21l6i3f 446 13 model model NN cord-001257-t21l6i3f 446 14 host host NN cord-001257-t21l6i3f 446 15 to to TO cord-001257-t21l6i3f 446 16 dissect dissect VB cord-001257-t21l6i3f 446 17 functions function NNS cord-001257-t21l6i3f 446 18 of of IN cord-001257-t21l6i3f 446 19 viral viral JJ cord-001257-t21l6i3f 446 20 and and CC cord-001257-t21l6i3f 446 21 host host NN cord-001257-t21l6i3f 446 22 factors factor NNS cord-001257-t21l6i3f 446 23 in in IN cord-001257-t21l6i3f 446 24 tombusvirus tombusvirus NN cord-001257-t21l6i3f 446 25 replication replication NN cord-001257-t21l6i3f 446 26 Host host NN cord-001257-t21l6i3f 446 27 factors factor NNS cord-001257-t21l6i3f 446 28 involved involve VBN cord-001257-t21l6i3f 446 29 in in IN cord-001257-t21l6i3f 446 30 West West NNP cord-001257-t21l6i3f 446 31 Nile Nile NNP cord-001257-t21l6i3f 446 32 virus virus NN cord-001257-t21l6i3f 446 33 replication replication NN cord-001257-t21l6i3f 446 34 Viral viral JJ cord-001257-t21l6i3f 446 35 and and CC cord-001257-t21l6i3f 446 36 cellular cellular JJ cord-001257-t21l6i3f 446 37 proteins protein NNS cord-001257-t21l6i3f 446 38 involved involve VBN cord-001257-t21l6i3f 446 39 in in IN cord-001257-t21l6i3f 446 40 coronavirus coronavirus NN cord-001257-t21l6i3f 446 41 replication replication NN cord-001257-t21l6i3f 446 42 Emerging emerge VBG cord-001257-t21l6i3f 446 43 picture picture NN cord-001257-t21l6i3f 446 44 of of IN cord-001257-t21l6i3f 446 45 host host NN cord-001257-t21l6i3f 446 46 chaperone chaperone NN cord-001257-t21l6i3f 446 47 and and CC cord-001257-t21l6i3f 446 48 cyclophilin cyclophilin NNP cord-001257-t21l6i3f 446 49 roles role NNS cord-001257-t21l6i3f 446 50 in in IN cord-001257-t21l6i3f 446 51 RNA RNA NNP cord-001257-t21l6i3f 446 52 virus virus NN cord-001257-t21l6i3f 446 53 replication replication NN cord-001257-t21l6i3f 446 54 Hepatitis Hepatitis NNP cord-001257-t21l6i3f 446 55 C C NNP cord-001257-t21l6i3f 446 56 virus virus NN cord-001257-t21l6i3f 446 57 replication replication NN cord-001257-t21l6i3f 446 58 cycle cycle NN cord-001257-t21l6i3f 446 59 Virus Virus NNP cord-001257-t21l6i3f 446 60 factories factory NNS cord-001257-t21l6i3f 446 61 : : : cord-001257-t21l6i3f 446 62 associations association NNS cord-001257-t21l6i3f 446 63 of of IN cord-001257-t21l6i3f 446 64 cell cell NN cord-001257-t21l6i3f 446 65 organelles organelle NNS cord-001257-t21l6i3f 446 66 for for IN cord-001257-t21l6i3f 446 67 viral viral JJ cord-001257-t21l6i3f 446 68 replication replication NN cord-001257-t21l6i3f 446 69 and and CC cord-001257-t21l6i3f 446 70 morphogenesis morphogenesis NN cord-001257-t21l6i3f 446 71 Virus virus NN cord-001257-t21l6i3f 446 72 factories factory NNS cord-001257-t21l6i3f 446 73 : : : cord-001257-t21l6i3f 446 74 biogenesis biogenesis NN cord-001257-t21l6i3f 446 75 and and CC cord-001257-t21l6i3f 446 76 structural structural JJ cord-001257-t21l6i3f 446 77 design design NN cord-001257-t21l6i3f 447 1 Diverse diverse JJ cord-001257-t21l6i3f 447 2 roles role NNS cord-001257-t21l6i3f 447 3 of of IN cord-001257-t21l6i3f 447 4 host host NN cord-001257-t21l6i3f 447 5 RNA RNA NNP cord-001257-t21l6i3f 447 6 binding bind VBG cord-001257-t21l6i3f 447 7 proteins protein NNS cord-001257-t21l6i3f 447 8 in in IN cord-001257-t21l6i3f 447 9 RNA RNA NNP cord-001257-t21l6i3f 447 10 virus virus NN cord-001257-t21l6i3f 447 11 replication replication NN cord-001257-t21l6i3f 447 12 Non Non NNP cord-001257-t21l6i3f 447 13 - - NNP cord-001257-t21l6i3f 447 14 Templated Templated NNP cord-001257-t21l6i3f 447 15 Functions Functions NNPS cord-001257-t21l6i3f 447 16 of of IN cord-001257-t21l6i3f 447 17 Viral Viral NNP cord-001257-t21l6i3f 447 18 RNA RNA NNP cord-001257-t21l6i3f 447 19 in in IN cord-001257-t21l6i3f 447 20 Picornavirus Picornavirus NNP cord-001257-t21l6i3f 447 21 Replication Replication NNP cord-001257-t21l6i3f 447 22 Yeast Yeast NNP cord-001257-t21l6i3f 447 23 as as IN cord-001257-t21l6i3f 447 24 a a DT cord-001257-t21l6i3f 447 25 model model NN cord-001257-t21l6i3f 447 26 host host NN cord-001257-t21l6i3f 448 1 to to TO cord-001257-t21l6i3f 448 2 study study VB cord-001257-t21l6i3f 448 3 replication replication NN cord-001257-t21l6i3f 448 4 and and CC cord-001257-t21l6i3f 448 5 recombination recombination NN cord-001257-t21l6i3f 448 6 of of IN cord-001257-t21l6i3f 448 7 defective defective JJ cord-001257-t21l6i3f 448 8 interfering interfere VBG cord-001257-t21l6i3f 448 9 RNA RNA NNP cord-001257-t21l6i3f 448 10 of of IN cord-001257-t21l6i3f 448 11 Tomato Tomato NNP cord-001257-t21l6i3f 448 12 bushy bushy JJ cord-001257-t21l6i3f 448 13 stunt stunt NN cord-001257-t21l6i3f 448 14 virus virus NN cord-001257-t21l6i3f 448 15 Purification purification NN cord-001257-t21l6i3f 448 16 of of IN cord-001257-t21l6i3f 448 17 the the DT cord-001257-t21l6i3f 448 18 cucumber cucumber NN cord-001257-t21l6i3f 448 19 necrosis necrosis NN cord-001257-t21l6i3f 448 20 virus virus NN cord-001257-t21l6i3f 448 21 replicase replicase NN cord-001257-t21l6i3f 448 22 from from IN cord-001257-t21l6i3f 448 23 yeast yeast NN cord-001257-t21l6i3f 448 24 cells cell NNS cord-001257-t21l6i3f 448 25 : : : cord-001257-t21l6i3f 448 26 role role NN cord-001257-t21l6i3f 448 27 of of IN cord-001257-t21l6i3f 448 28 coexpressed coexpresse VBN cord-001257-t21l6i3f 448 29 viral viral JJ cord-001257-t21l6i3f 448 30 RNA RNA NNP cord-001257-t21l6i3f 448 31 in in IN cord-001257-t21l6i3f 448 32 stimulation stimulation NN cord-001257-t21l6i3f 448 33 of of IN cord-001257-t21l6i3f 448 34 replicase replicase NN cord-001257-t21l6i3f 448 35 activity activity NN cord-001257-t21l6i3f 448 36 Role role NN cord-001257-t21l6i3f 448 37 of of IN cord-001257-t21l6i3f 448 38 an an DT cord-001257-t21l6i3f 448 39 internal internal JJ cord-001257-t21l6i3f 448 40 and and CC cord-001257-t21l6i3f 448 41 two two CD cord-001257-t21l6i3f 448 42 39-terminal 39-terminal CD cord-001257-t21l6i3f 448 43 RNA RNA NNP cord-001257-t21l6i3f 448 44 elements element NNS cord-001257-t21l6i3f 448 45 in in IN cord-001257-t21l6i3f 448 46 assembly assembly NN cord-001257-t21l6i3f 448 47 of of IN cord-001257-t21l6i3f 448 48 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 448 49 replicase replicase NNP cord-001257-t21l6i3f 448 50 Specific Specific NNP cord-001257-t21l6i3f 448 51 Binding Binding NNP cord-001257-t21l6i3f 448 52 of of IN cord-001257-t21l6i3f 448 53 Tombusvirus Tombusvirus NNP cord-001257-t21l6i3f 448 54 Replication Replication NNP cord-001257-t21l6i3f 448 55 Protein Protein NNP cord-001257-t21l6i3f 448 56 p33 p33 NNP cord-001257-t21l6i3f 448 57 to to IN cord-001257-t21l6i3f 448 58 an an DT cord-001257-t21l6i3f 448 59 Internal Internal NNP cord-001257-t21l6i3f 448 60 Replication Replication NNP cord-001257-t21l6i3f 448 61 Element Element NNP cord-001257-t21l6i3f 448 62 in in IN cord-001257-t21l6i3f 448 63 the the DT cord-001257-t21l6i3f 448 64 Viral Viral NNP cord-001257-t21l6i3f 448 65 RNA RNA NNP cord-001257-t21l6i3f 448 66 Is be VBZ cord-001257-t21l6i3f 448 67 Essential Essential NNP cord-001257-t21l6i3f 448 68 for for IN cord-001257-t21l6i3f 448 69 Replication replication NN cord-001257-t21l6i3f 448 70 Multiple multiple JJ cord-001257-t21l6i3f 448 71 roles role NNS cord-001257-t21l6i3f 448 72 of of IN cord-001257-t21l6i3f 448 73 viral viral JJ cord-001257-t21l6i3f 448 74 replication replication NN cord-001257-t21l6i3f 448 75 proteins protein NNS cord-001257-t21l6i3f 448 76 in in IN cord-001257-t21l6i3f 448 77 plant plant NN cord-001257-t21l6i3f 448 78 RNA RNA NNP cord-001257-t21l6i3f 448 79 virus virus NN cord-001257-t21l6i3f 448 80 replication replication NN cord-001257-t21l6i3f 448 81 Defining define VBG cord-001257-t21l6i3f 448 82 the the DT cord-001257-t21l6i3f 448 83 Roles Roles NNPS cord-001257-t21l6i3f 448 84 of of IN cord-001257-t21l6i3f 448 85 cis cis NNP cord-001257-t21l6i3f 448 86 - - HYPH cord-001257-t21l6i3f 448 87 Acting Acting NNP cord-001257-t21l6i3f 448 88 RNA RNA NNP cord-001257-t21l6i3f 448 89 Elements Elements NNPS cord-001257-t21l6i3f 448 90 in in IN cord-001257-t21l6i3f 448 91 Tombusvirus Tombusvirus NNP cord-001257-t21l6i3f 448 92 Replicase Replicase NNP cord-001257-t21l6i3f 448 93 Assembly Assembly NNP cord-001257-t21l6i3f 449 1 In in IN cord-001257-t21l6i3f 450 1 Vitro Vitro NNP cord-001257-t21l6i3f 450 2 A a DT cord-001257-t21l6i3f 450 3 discontinuous discontinuous JJ cord-001257-t21l6i3f 450 4 RNA RNA NNP cord-001257-t21l6i3f 450 5 platform platform NN cord-001257-t21l6i3f 450 6 mediates mediate VBZ cord-001257-t21l6i3f 450 7 RNA RNA NNP cord-001257-t21l6i3f 450 8 virus virus NN cord-001257-t21l6i3f 450 9 replication replication NN cord-001257-t21l6i3f 450 10 : : : cord-001257-t21l6i3f 450 11 building build VBG cord-001257-t21l6i3f 450 12 an an DT cord-001257-t21l6i3f 450 13 integrated integrate VBN cord-001257-t21l6i3f 450 14 model model NN cord-001257-t21l6i3f 450 15 for for IN cord-001257-t21l6i3f 450 16 RNA RNA NNP cord-001257-t21l6i3f 450 17 - - HYPH cord-001257-t21l6i3f 450 18 based base VBN cord-001257-t21l6i3f 450 19 regulation regulation NN cord-001257-t21l6i3f 450 20 of of IN cord-001257-t21l6i3f 450 21 viral viral JJ cord-001257-t21l6i3f 450 22 processes process NNS cord-001257-t21l6i3f 451 1 Tomato tomato NN cord-001257-t21l6i3f 451 2 bushy bushy JJ cord-001257-t21l6i3f 451 3 stunt stunt NN cord-001257-t21l6i3f 451 4 virus virus NN cord-001257-t21l6i3f 451 5 Co co NN cord-001257-t21l6i3f 451 6 - - NN cord-001257-t21l6i3f 451 7 Opts opt VBZ cord-001257-t21l6i3f 451 8 the the DT cord-001257-t21l6i3f 451 9 RNA RNA NNP cord-001257-t21l6i3f 451 10 - - HYPH cord-001257-t21l6i3f 451 11 Binding Binding NNP cord-001257-t21l6i3f 451 12 Function Function NNP cord-001257-t21l6i3f 451 13 of of IN cord-001257-t21l6i3f 451 14 a a DT cord-001257-t21l6i3f 451 15 Host Host NNP cord-001257-t21l6i3f 451 16 Metabolic Metabolic NNP cord-001257-t21l6i3f 451 17 Enzyme Enzyme NNP cord-001257-t21l6i3f 451 18 for for IN cord-001257-t21l6i3f 451 19 Viral Viral NNP cord-001257-t21l6i3f 451 20 Genomic Genomic NNP cord-001257-t21l6i3f 451 21 RNA RNA NNP cord-001257-t21l6i3f 451 22 Synthesis Synthesis NNP cord-001257-t21l6i3f 451 23 A A NNP cord-001257-t21l6i3f 451 24 temperature temperature NN cord-001257-t21l6i3f 451 25 sensitive sensitive JJ cord-001257-t21l6i3f 451 26 mutant mutant NN cord-001257-t21l6i3f 451 27 of of IN cord-001257-t21l6i3f 451 28 heat heat NN cord-001257-t21l6i3f 451 29 shock shock NN cord-001257-t21l6i3f 451 30 protein protein NN cord-001257-t21l6i3f 451 31 70 70 CD cord-001257-t21l6i3f 451 32 reveals reveal VBZ cord-001257-t21l6i3f 451 33 an an DT cord-001257-t21l6i3f 451 34 essential essential JJ cord-001257-t21l6i3f 451 35 role role NN cord-001257-t21l6i3f 451 36 during during IN cord-001257-t21l6i3f 451 37 the the DT cord-001257-t21l6i3f 451 38 early early JJ cord-001257-t21l6i3f 451 39 steps step NNS cord-001257-t21l6i3f 451 40 of of IN cord-001257-t21l6i3f 451 41 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 451 42 replication replication NN cord-001257-t21l6i3f 452 1 A a DT cord-001257-t21l6i3f 452 2 key key JJ cord-001257-t21l6i3f 452 3 role role NN cord-001257-t21l6i3f 452 4 for for IN cord-001257-t21l6i3f 452 5 heat heat NN cord-001257-t21l6i3f 452 6 shock shock NN cord-001257-t21l6i3f 452 7 protein protein NN cord-001257-t21l6i3f 452 8 70 70 CD cord-001257-t21l6i3f 452 9 in in IN cord-001257-t21l6i3f 452 10 the the DT cord-001257-t21l6i3f 452 11 localization localization NN cord-001257-t21l6i3f 452 12 and and CC cord-001257-t21l6i3f 452 13 insertion insertion NN cord-001257-t21l6i3f 452 14 of of IN cord-001257-t21l6i3f 452 15 tombusvirus tombusvirus NN cord-001257-t21l6i3f 452 16 replication replication NN cord-001257-t21l6i3f 452 17 proteins protein NNS cord-001257-t21l6i3f 452 18 to to IN cord-001257-t21l6i3f 452 19 intracellular intracellular JJ cord-001257-t21l6i3f 452 20 membranes membrane NNS cord-001257-t21l6i3f 453 1 In in IN cord-001257-t21l6i3f 453 2 vitro vitro FW cord-001257-t21l6i3f 453 3 assembly assembly NN cord-001257-t21l6i3f 453 4 of of IN cord-001257-t21l6i3f 453 5 the the DT cord-001257-t21l6i3f 453 6 Tomato Tomato NNP cord-001257-t21l6i3f 453 7 bushy bushy JJ cord-001257-t21l6i3f 453 8 stunt stunt NN cord-001257-t21l6i3f 453 9 virus virus NN cord-001257-t21l6i3f 453 10 replicase replicase NNP cord-001257-t21l6i3f 453 11 requires require VBZ cord-001257-t21l6i3f 453 12 the the DT cord-001257-t21l6i3f 453 13 host host NN cord-001257-t21l6i3f 453 14 Heat Heat NNP cord-001257-t21l6i3f 453 15 shock shock NN cord-001257-t21l6i3f 453 16 protein protein NN cord-001257-t21l6i3f 453 17 70 70 CD cord-001257-t21l6i3f 453 18 Proteomics Proteomics NNP cord-001257-t21l6i3f 453 19 analysis analysis NN cord-001257-t21l6i3f 453 20 of of IN cord-001257-t21l6i3f 453 21 the the DT cord-001257-t21l6i3f 453 22 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 453 23 replicase replicase NNP cord-001257-t21l6i3f 453 24 : : : cord-001257-t21l6i3f 454 1 Hsp70 Hsp70 NNP cord-001257-t21l6i3f 454 2 molecular molecular JJ cord-001257-t21l6i3f 454 3 chaperone chaperone NN cord-001257-t21l6i3f 454 4 is be VBZ cord-001257-t21l6i3f 454 5 associated associate VBN cord-001257-t21l6i3f 454 6 with with IN cord-001257-t21l6i3f 454 7 the the DT cord-001257-t21l6i3f 454 8 replicase replicase NN cord-001257-t21l6i3f 454 9 and and CC cord-001257-t21l6i3f 454 10 enhances enhance VBZ cord-001257-t21l6i3f 454 11 viral viral JJ cord-001257-t21l6i3f 454 12 RNA RNA NNP cord-001257-t21l6i3f 454 13 replication replication NN cord-001257-t21l6i3f 454 14 Ubiquitin Ubiquitin NNP cord-001257-t21l6i3f 454 15 - - HYPH cord-001257-t21l6i3f 454 16 Conjugating Conjugating NNP cord-001257-t21l6i3f 454 17 Enzyme Enzyme NNP cord-001257-t21l6i3f 454 18 Is be VBZ cord-001257-t21l6i3f 454 19 a a DT cord-001257-t21l6i3f 454 20 Component Component NNP cord-001257-t21l6i3f 454 21 of of IN cord-001257-t21l6i3f 454 22 the the DT cord-001257-t21l6i3f 454 23 Tombusvirus Tombusvirus NNP cord-001257-t21l6i3f 454 24 Replicase Replicase NNP cord-001257-t21l6i3f 454 25 Complex Complex NNP cord-001257-t21l6i3f 454 26 and and CC cord-001257-t21l6i3f 454 27 Ubiquitinates Ubiquitinates NNP cord-001257-t21l6i3f 454 28 p33 p33 NNP cord-001257-t21l6i3f 454 29 Replication Replication NNP cord-001257-t21l6i3f 454 30 Protein Protein NNP cord-001257-t21l6i3f 454 31 Translation Translation NNP cord-001257-t21l6i3f 454 32 elongation elongation NN cord-001257-t21l6i3f 454 33 factor factor NN cord-001257-t21l6i3f 454 34 1A 1a NN cord-001257-t21l6i3f 454 35 facilitates facilitate VBZ cord-001257-t21l6i3f 454 36 the the DT cord-001257-t21l6i3f 454 37 assembly assembly NN cord-001257-t21l6i3f 454 38 of of IN cord-001257-t21l6i3f 454 39 the the DT cord-001257-t21l6i3f 454 40 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 454 41 replicase replicase NN cord-001257-t21l6i3f 454 42 and and CC cord-001257-t21l6i3f 454 43 stimulates stimulate VBZ cord-001257-t21l6i3f 454 44 minus minus JJ cord-001257-t21l6i3f 454 45 - - HYPH cord-001257-t21l6i3f 454 46 strand strand NN cord-001257-t21l6i3f 454 47 synthesis synthesis NN cord-001257-t21l6i3f 455 1 Translation translation NN cord-001257-t21l6i3f 455 2 elongation elongation NN cord-001257-t21l6i3f 455 3 factor factor NN cord-001257-t21l6i3f 455 4 1A 1a NN cord-001257-t21l6i3f 455 5 is be VBZ cord-001257-t21l6i3f 455 6 a a DT cord-001257-t21l6i3f 455 7 component component NN cord-001257-t21l6i3f 455 8 of of IN cord-001257-t21l6i3f 455 9 the the DT cord-001257-t21l6i3f 455 10 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 455 11 replicase replicase NNP cord-001257-t21l6i3f 455 12 complex complex NN cord-001257-t21l6i3f 455 13 and and CC cord-001257-t21l6i3f 455 14 affects affect VBZ cord-001257-t21l6i3f 455 15 the the DT cord-001257-t21l6i3f 455 16 stability stability NN cord-001257-t21l6i3f 455 17 of of IN cord-001257-t21l6i3f 455 18 the the DT cord-001257-t21l6i3f 455 19 p33 p33 CD cord-001257-t21l6i3f 455 20 replication replication NN cord-001257-t21l6i3f 455 21 co co NN cord-001257-t21l6i3f 455 22 - - NN cord-001257-t21l6i3f 455 23 factor factor NN cord-001257-t21l6i3f 455 24 Synergistic synergistic JJ cord-001257-t21l6i3f 455 25 Roles Roles NNPS cord-001257-t21l6i3f 455 26 of of IN cord-001257-t21l6i3f 455 27 Eukaryotic eukaryotic JJ cord-001257-t21l6i3f 455 28 Translation Translation NNP cord-001257-t21l6i3f 455 29 Elongation Elongation NNP cord-001257-t21l6i3f 455 30 Factors Factors NNP cord-001257-t21l6i3f 455 31 1Bgamma 1bgamma CD cord-001257-t21l6i3f 455 32 and and CC cord-001257-t21l6i3f 455 33 1A 1a CD cord-001257-t21l6i3f 455 34 in in IN cord-001257-t21l6i3f 455 35 Stimulation stimulation NN cord-001257-t21l6i3f 455 36 of of IN cord-001257-t21l6i3f 455 37 Tombusvirus Tombusvirus NNP cord-001257-t21l6i3f 455 38 Minus Minus NNP cord-001257-t21l6i3f 455 39 - - HYPH cord-001257-t21l6i3f 455 40 Strand Strand NNP cord-001257-t21l6i3f 455 41 Synthesis Synthesis NNP cord-001257-t21l6i3f 455 42 A a NN cord-001257-t21l6i3f 456 1 Co Co NNP cord-001257-t21l6i3f 456 2 - - JJ cord-001257-t21l6i3f 456 3 Opted Opted NNP cord-001257-t21l6i3f 456 4 DEAD DEAD NNP cord-001257-t21l6i3f 456 5 - - HYPH cord-001257-t21l6i3f 456 6 Box Box NNP cord-001257-t21l6i3f 456 7 RNA RNA NNP cord-001257-t21l6i3f 456 8 Helicase Helicase NNP cord-001257-t21l6i3f 456 9 Enhances enhance VBZ cord-001257-t21l6i3f 456 10 Tombusvirus Tombusvirus NNP cord-001257-t21l6i3f 456 11 Plus Plus NNP cord-001257-t21l6i3f 456 12 - - HYPH cord-001257-t21l6i3f 456 13 Strand Strand NNP cord-001257-t21l6i3f 456 14 Synthesis Synthesis NNP cord-001257-t21l6i3f 457 1 The the DT cord-001257-t21l6i3f 457 2 host host NN cord-001257-t21l6i3f 457 3 Pex19p Pex19p NNP cord-001257-t21l6i3f 457 4 plays play VBZ cord-001257-t21l6i3f 457 5 a a DT cord-001257-t21l6i3f 457 6 role role NN cord-001257-t21l6i3f 457 7 in in IN cord-001257-t21l6i3f 457 8 peroxisomal peroxisomal JJ cord-001257-t21l6i3f 457 9 localization localization NN cord-001257-t21l6i3f 457 10 of of IN cord-001257-t21l6i3f 458 1 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 458 2 replication replication NN cord-001257-t21l6i3f 458 3 proteins protein VBZ cord-001257-t21l6i3f 458 4 Ubiquitination Ubiquitination NNP cord-001257-t21l6i3f 458 5 of of IN cord-001257-t21l6i3f 458 6 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 458 7 p33 p33 NNP cord-001257-t21l6i3f 458 8 replication replication NN cord-001257-t21l6i3f 458 9 protein protein NN cord-001257-t21l6i3f 458 10 plays play VBZ cord-001257-t21l6i3f 458 11 a a DT cord-001257-t21l6i3f 458 12 role role NN cord-001257-t21l6i3f 458 13 in in IN cord-001257-t21l6i3f 458 14 virus virus NN cord-001257-t21l6i3f 458 15 replication replication NN cord-001257-t21l6i3f 458 16 and and CC cord-001257-t21l6i3f 458 17 binding bind VBG cord-001257-t21l6i3f 458 18 to to IN cord-001257-t21l6i3f 458 19 the the DT cord-001257-t21l6i3f 458 20 host host NN cord-001257-t21l6i3f 458 21 Vps23p Vps23p NNP cord-001257-t21l6i3f 458 22 ESCRT ESCRT NNP cord-001257-t21l6i3f 458 23 protein protein NN cord-001257-t21l6i3f 459 1 A a DT cord-001257-t21l6i3f 459 2 Unique unique JJ cord-001257-t21l6i3f 459 3 Role role NN cord-001257-t21l6i3f 459 4 for for IN cord-001257-t21l6i3f 459 5 the the DT cord-001257-t21l6i3f 459 6 Host Host NNP cord-001257-t21l6i3f 459 7 ESCRT ESCRT NNP cord-001257-t21l6i3f 459 8 Proteins protein NNS cord-001257-t21l6i3f 459 9 in in IN cord-001257-t21l6i3f 459 10 Replication Replication NNP cord-001257-t21l6i3f 459 11 of of IN cord-001257-t21l6i3f 459 12 Tomato Tomato NNP cord-001257-t21l6i3f 459 13 bushy bushy JJ cord-001257-t21l6i3f 459 14 stunt stunt NN cord-001257-t21l6i3f 459 15 virus virus NN cord-001257-t21l6i3f 459 16 Global global JJ cord-001257-t21l6i3f 459 17 genomics genomic NNS cord-001257-t21l6i3f 459 18 and and CC cord-001257-t21l6i3f 459 19 proteomics proteomic NNS cord-001257-t21l6i3f 459 20 approaches approach NNS cord-001257-t21l6i3f 459 21 to to TO cord-001257-t21l6i3f 459 22 identify identify VB cord-001257-t21l6i3f 459 23 host host NN cord-001257-t21l6i3f 459 24 factors factor NNS cord-001257-t21l6i3f 459 25 as as IN cord-001257-t21l6i3f 459 26 targets target NNS cord-001257-t21l6i3f 459 27 to to TO cord-001257-t21l6i3f 459 28 induce induce VB cord-001257-t21l6i3f 459 29 resistance resistance NN cord-001257-t21l6i3f 459 30 against against IN cord-001257-t21l6i3f 459 31 tomato tomato NN cord-001257-t21l6i3f 459 32 bushy bushy JJ cord-001257-t21l6i3f 459 33 stunt stunt NN cord-001257-t21l6i3f 459 34 virus virus NN cord-001257-t21l6i3f 460 1 Yeast yeast NN cord-001257-t21l6i3f 460 2 genome genome NN cord-001257-t21l6i3f 460 3 - - HYPH cord-001257-t21l6i3f 460 4 wide wide JJ cord-001257-t21l6i3f 460 5 screen screen NN cord-001257-t21l6i3f 460 6 reveals reveal VBZ cord-001257-t21l6i3f 460 7 dissimilar dissimilar JJ cord-001257-t21l6i3f 460 8 sets set NNS cord-001257-t21l6i3f 460 9 of of IN cord-001257-t21l6i3f 460 10 host host NN cord-001257-t21l6i3f 460 11 genes gene NNS cord-001257-t21l6i3f 460 12 affecting affect VBG cord-001257-t21l6i3f 460 13 replication replication NN cord-001257-t21l6i3f 460 14 of of IN cord-001257-t21l6i3f 460 15 RNA RNA NNP cord-001257-t21l6i3f 460 16 viruses virus NNS cord-001257-t21l6i3f 460 17 Identification Identification NNP cord-001257-t21l6i3f 460 18 of of IN cord-001257-t21l6i3f 460 19 essential essential JJ cord-001257-t21l6i3f 460 20 host host NN cord-001257-t21l6i3f 460 21 factors factor NNS cord-001257-t21l6i3f 460 22 affecting affect VBG cord-001257-t21l6i3f 460 23 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 460 24 RNA RNA NNP cord-001257-t21l6i3f 460 25 replication replication NN cord-001257-t21l6i3f 460 26 based base VBN cord-001257-t21l6i3f 460 27 on on IN cord-001257-t21l6i3f 460 28 the the DT cord-001257-t21l6i3f 460 29 yeast yeast NN cord-001257-t21l6i3f 460 30 Tet Tet NNP cord-001257-t21l6i3f 460 31 promoters promoter NNS cord-001257-t21l6i3f 460 32 Hughes Hughes NNP cord-001257-t21l6i3f 460 33 Collection Collection NNP cord-001257-t21l6i3f 461 1 The the DT cord-001257-t21l6i3f 461 2 roles role NNS cord-001257-t21l6i3f 461 3 of of IN cord-001257-t21l6i3f 461 4 host host NN cord-001257-t21l6i3f 461 5 factors factor NNS cord-001257-t21l6i3f 461 6 in in IN cord-001257-t21l6i3f 461 7 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 461 8 RNA RNA NNP cord-001257-t21l6i3f 461 9 recombination recombination NN cord-001257-t21l6i3f 461 10 Cpr1 Cpr1 NNP cord-001257-t21l6i3f 461 11 cyclophilin cyclophilin NN cord-001257-t21l6i3f 461 12 and and CC cord-001257-t21l6i3f 461 13 Ess1 Ess1 NNP cord-001257-t21l6i3f 461 14 parvulin parvulin NNP cord-001257-t21l6i3f 461 15 prolyl prolyl FW cord-001257-t21l6i3f 461 16 isomerases isomerases NNPS cord-001257-t21l6i3f 461 17 interact interact VBP cord-001257-t21l6i3f 461 18 with with IN cord-001257-t21l6i3f 461 19 the the DT cord-001257-t21l6i3f 461 20 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 461 21 replication replication NN cord-001257-t21l6i3f 461 22 protein protein NN cord-001257-t21l6i3f 461 23 and and CC cord-001257-t21l6i3f 461 24 inhibit inhibit VB cord-001257-t21l6i3f 461 25 viral viral JJ cord-001257-t21l6i3f 461 26 replication replication NN cord-001257-t21l6i3f 461 27 in in IN cord-001257-t21l6i3f 461 28 yeast yeast NN cord-001257-t21l6i3f 461 29 model model NN cord-001257-t21l6i3f 461 30 host host NN cord-001257-t21l6i3f 461 31 mRNA mRNA NNP cord-001257-t21l6i3f 461 32 export export NN cord-001257-t21l6i3f 461 33 : : : cord-001257-t21l6i3f 462 1 RNP rnp NN cord-001257-t21l6i3f 462 2 remodeling remodeling NN cord-001257-t21l6i3f 462 3 by by IN cord-001257-t21l6i3f 462 4 DEAD DEAD NNP cord-001257-t21l6i3f 462 5 - - HYPH cord-001257-t21l6i3f 462 6 box box NN cord-001257-t21l6i3f 462 7 proteins protein NNS cord-001257-t21l6i3f 462 8 Bent bend VBN cord-001257-t21l6i3f 462 9 out out IN cord-001257-t21l6i3f 462 10 of of IN cord-001257-t21l6i3f 462 11 shape shape NN cord-001257-t21l6i3f 462 12 : : : cord-001257-t21l6i3f 462 13 RNA rna NN cord-001257-t21l6i3f 462 14 unwinding unwind VBG cord-001257-t21l6i3f 462 15 by by IN cord-001257-t21l6i3f 462 16 the the DT cord-001257-t21l6i3f 462 17 DEAD DEAD NNP cord-001257-t21l6i3f 462 18 - - HYPH cord-001257-t21l6i3f 462 19 box box NN cord-001257-t21l6i3f 462 20 helicase helicase NN cord-001257-t21l6i3f 462 21 Vasa Vasa NNP cord-001257-t21l6i3f 463 1 The the DT cord-001257-t21l6i3f 463 2 DEAD DEAD NNP cord-001257-t21l6i3f 463 3 - - HYPH cord-001257-t21l6i3f 463 4 box box NN cord-001257-t21l6i3f 463 5 protein protein NN cord-001257-t21l6i3f 463 6 family family NN cord-001257-t21l6i3f 463 7 of of IN cord-001257-t21l6i3f 463 8 RNA RNA NNP cord-001257-t21l6i3f 463 9 helicases helicases NNPS cord-001257-t21l6i3f 463 10 RNA RNA NNP cord-001257-t21l6i3f 463 11 helicases helicase NNS cord-001257-t21l6i3f 463 12 : : : cord-001257-t21l6i3f 463 13 emerging emerge VBG cord-001257-t21l6i3f 463 14 roles role NNS cord-001257-t21l6i3f 463 15 in in IN cord-001257-t21l6i3f 463 16 viral viral JJ cord-001257-t21l6i3f 463 17 replication replication NN cord-001257-t21l6i3f 463 18 and and CC cord-001257-t21l6i3f 463 19 the the DT cord-001257-t21l6i3f 463 20 host host NN cord-001257-t21l6i3f 463 21 innate innate JJ cord-001257-t21l6i3f 463 22 response response NN cord-001257-t21l6i3f 464 1 A a DT cord-001257-t21l6i3f 464 2 host host NN cord-001257-t21l6i3f 464 3 RNA rna NN cord-001257-t21l6i3f 464 4 helicase helicase NN cord-001257-t21l6i3f 464 5 - - HYPH cord-001257-t21l6i3f 464 6 like like JJ cord-001257-t21l6i3f 464 7 protein protein NN cord-001257-t21l6i3f 464 8 , , , cord-001257-t21l6i3f 464 9 AtRH8 AtRH8 NNP cord-001257-t21l6i3f 464 10 , , , cord-001257-t21l6i3f 464 11 interacts interact VBZ cord-001257-t21l6i3f 464 12 with with IN cord-001257-t21l6i3f 464 13 the the DT cord-001257-t21l6i3f 464 14 potyviral potyviral NNP cord-001257-t21l6i3f 464 15 genome genome NN cord-001257-t21l6i3f 464 16 - - HYPH cord-001257-t21l6i3f 464 17 linked link VBN cord-001257-t21l6i3f 464 18 protein protein NN cord-001257-t21l6i3f 464 19 , , , cord-001257-t21l6i3f 464 20 VPg VPg NNS cord-001257-t21l6i3f 464 21 , , , cord-001257-t21l6i3f 464 22 associates associate NNS cord-001257-t21l6i3f 464 23 with with IN cord-001257-t21l6i3f 464 24 the the DT cord-001257-t21l6i3f 464 25 virus virus NN cord-001257-t21l6i3f 464 26 accumulation accumulation NN cord-001257-t21l6i3f 464 27 complex complex NN cord-001257-t21l6i3f 464 28 , , , cord-001257-t21l6i3f 464 29 and and CC cord-001257-t21l6i3f 464 30 is be VBZ cord-001257-t21l6i3f 464 31 essential essential JJ cord-001257-t21l6i3f 464 32 for for IN cord-001257-t21l6i3f 464 33 infection infection NN cord-001257-t21l6i3f 464 34 Genome genome NN cord-001257-t21l6i3f 464 35 - - HYPH cord-001257-t21l6i3f 464 36 wide wide JJ cord-001257-t21l6i3f 464 37 analysis analysis NN cord-001257-t21l6i3f 464 38 of of IN cord-001257-t21l6i3f 464 39 helicase helicase NNP cord-001257-t21l6i3f 464 40 gene gene NN cord-001257-t21l6i3f 464 41 family family NN cord-001257-t21l6i3f 464 42 from from IN cord-001257-t21l6i3f 464 43 rice rice NN cord-001257-t21l6i3f 464 44 and and CC cord-001257-t21l6i3f 464 45 Arabidopsis Arabidopsis NNP cord-001257-t21l6i3f 464 46 : : : cord-001257-t21l6i3f 464 47 a a DT cord-001257-t21l6i3f 464 48 comparison comparison NN cord-001257-t21l6i3f 464 49 with with IN cord-001257-t21l6i3f 464 50 yeast yeast NN cord-001257-t21l6i3f 464 51 and and CC cord-001257-t21l6i3f 464 52 human human JJ cord-001257-t21l6i3f 464 53 STRESS stress NN cord-001257-t21l6i3f 464 54 RESPONSE response NN cord-001257-t21l6i3f 464 55 SUPPRESSOR1 suppressor1 NN cord-001257-t21l6i3f 464 56 and and CC cord-001257-t21l6i3f 464 57 STRESS stress NN cord-001257-t21l6i3f 464 58 RESPONSE response NN cord-001257-t21l6i3f 464 59 SUPPRESSOR2 suppressor2 NN cord-001257-t21l6i3f 464 60 , , , cord-001257-t21l6i3f 464 61 two two CD cord-001257-t21l6i3f 464 62 DEAD dead JJ cord-001257-t21l6i3f 464 63 - - HYPH cord-001257-t21l6i3f 464 64 box box NN cord-001257-t21l6i3f 464 65 RNA RNA NNP cord-001257-t21l6i3f 464 66 helicases helicase NNS cord-001257-t21l6i3f 464 67 that that WDT cord-001257-t21l6i3f 464 68 attenuate attenuate VBP cord-001257-t21l6i3f 464 69 Arabidopsis Arabidopsis NNP cord-001257-t21l6i3f 464 70 responses response NNS cord-001257-t21l6i3f 464 71 to to IN cord-001257-t21l6i3f 464 72 multiple multiple JJ cord-001257-t21l6i3f 464 73 abiotic abiotic JJ cord-001257-t21l6i3f 464 74 stresses stress NNS cord-001257-t21l6i3f 464 75 SDE3 SDE3 NNP cord-001257-t21l6i3f 464 76 encodes encode VBZ cord-001257-t21l6i3f 464 77 an an DT cord-001257-t21l6i3f 464 78 RNA RNA NNP cord-001257-t21l6i3f 464 79 helicase helicase NN cord-001257-t21l6i3f 464 80 required require VBN cord-001257-t21l6i3f 464 81 for for IN cord-001257-t21l6i3f 464 82 post post JJ cord-001257-t21l6i3f 464 83 - - JJ cord-001257-t21l6i3f 464 84 transcriptional transcriptional JJ cord-001257-t21l6i3f 464 85 gene gene NN cord-001257-t21l6i3f 464 86 silencing silencing NN cord-001257-t21l6i3f 464 87 in in IN cord-001257-t21l6i3f 464 88 Arabidopsis Arabidopsis NNP cord-001257-t21l6i3f 464 89 Evolution Evolution NNP cord-001257-t21l6i3f 464 90 and and CC cord-001257-t21l6i3f 464 91 taxonomy taxonomy NN cord-001257-t21l6i3f 464 92 of of IN cord-001257-t21l6i3f 464 93 positive positive JJ cord-001257-t21l6i3f 464 94 - - HYPH cord-001257-t21l6i3f 464 95 strand strand NN cord-001257-t21l6i3f 464 96 RNA RNA NNP cord-001257-t21l6i3f 464 97 viruses virus NNS cord-001257-t21l6i3f 464 98 : : : cord-001257-t21l6i3f 464 99 implications implication NNS cord-001257-t21l6i3f 464 100 of of IN cord-001257-t21l6i3f 464 101 comparative comparative JJ cord-001257-t21l6i3f 464 102 analysis analysis NN cord-001257-t21l6i3f 464 103 of of IN cord-001257-t21l6i3f 464 104 amino amino NN cord-001257-t21l6i3f 464 105 acid acid NN cord-001257-t21l6i3f 464 106 sequences sequence NNS cord-001257-t21l6i3f 464 107 Role Role NNP cord-001257-t21l6i3f 464 108 of of IN cord-001257-t21l6i3f 464 109 RNA RNA NNP cord-001257-t21l6i3f 464 110 chaperones chaperone NNS cord-001257-t21l6i3f 464 111 in in IN cord-001257-t21l6i3f 464 112 virus virus NN cord-001257-t21l6i3f 464 113 replication replication NN cord-001257-t21l6i3f 465 1 Similar similar JJ cord-001257-t21l6i3f 465 2 roles role NNS cord-001257-t21l6i3f 465 3 for for IN cord-001257-t21l6i3f 465 4 yeast yeast NN cord-001257-t21l6i3f 465 5 Dbp2 Dbp2 NNP cord-001257-t21l6i3f 465 6 and and CC cord-001257-t21l6i3f 465 7 Arabidopsis Arabidopsis NNP cord-001257-t21l6i3f 465 8 RH20 RH20 NNP cord-001257-t21l6i3f 465 9 DEAD DEAD NNP cord-001257-t21l6i3f 465 10 - - HYPH cord-001257-t21l6i3f 465 11 box box NN cord-001257-t21l6i3f 465 12 RNA RNA NNP cord-001257-t21l6i3f 465 13 helicases helicase NNS cord-001257-t21l6i3f 465 14 to to IN cord-001257-t21l6i3f 465 15 Ded1 Ded1 NNP cord-001257-t21l6i3f 465 16 helicase helicase NN cord-001257-t21l6i3f 465 17 in in IN cord-001257-t21l6i3f 465 18 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 465 19 plus plus CC cord-001257-t21l6i3f 465 20 - - HYPH cord-001257-t21l6i3f 465 21 strand strand NN cord-001257-t21l6i3f 465 22 synthesis synthesis NN cord-001257-t21l6i3f 465 23 Dbp3p Dbp3p NNP cord-001257-t21l6i3f 465 24 , , , cord-001257-t21l6i3f 465 25 a a DT cord-001257-t21l6i3f 465 26 putative putative JJ cord-001257-t21l6i3f 465 27 RNA RNA NNP cord-001257-t21l6i3f 465 28 helicase helicase NN cord-001257-t21l6i3f 465 29 in in IN cord-001257-t21l6i3f 465 30 Saccharomyces Saccharomyces NNP cord-001257-t21l6i3f 465 31 cerevisiae cerevisiae NNS cord-001257-t21l6i3f 465 32 , , , cord-001257-t21l6i3f 465 33 is be VBZ cord-001257-t21l6i3f 465 34 required require VBN cord-001257-t21l6i3f 465 35 for for IN cord-001257-t21l6i3f 465 36 efficient efficient JJ cord-001257-t21l6i3f 465 37 pre pre JJ cord-001257-t21l6i3f 465 38 - - JJ cord-001257-t21l6i3f 465 39 rRNA rrna JJ cord-001257-t21l6i3f 465 40 processing processing NN cord-001257-t21l6i3f 465 41 predominantly predominantly RB cord-001257-t21l6i3f 465 42 at at IN cord-001257-t21l6i3f 465 43 site site NN cord-001257-t21l6i3f 465 44 A3 A3 NNP cord-001257-t21l6i3f 465 45 Duplex Duplex NNP cord-001257-t21l6i3f 465 46 destabilization destabilization NN cord-001257-t21l6i3f 465 47 by by IN cord-001257-t21l6i3f 465 48 four four CD cord-001257-t21l6i3f 465 49 ribosomal ribosomal JJ cord-001257-t21l6i3f 465 50 DEAD DEAD NNP cord-001257-t21l6i3f 465 51 - - HYPH cord-001257-t21l6i3f 465 52 box box NN cord-001257-t21l6i3f 465 53 proteins protein NNS cord-001257-t21l6i3f 466 1 Human human NN cord-001257-t21l6i3f 466 2 eIF4AIII eIF4AIII NNS cord-001257-t21l6i3f 466 3 interacts interact VBZ cord-001257-t21l6i3f 466 4 with with IN cord-001257-t21l6i3f 466 5 an an DT cord-001257-t21l6i3f 466 6 eIF4G eif4g CD cord-001257-t21l6i3f 466 7 - - HYPH cord-001257-t21l6i3f 466 8 like like JJ cord-001257-t21l6i3f 466 9 partner partner NN cord-001257-t21l6i3f 466 10 , , , cord-001257-t21l6i3f 466 11 NOM1 NOM1 NNP cord-001257-t21l6i3f 466 12 , , , cord-001257-t21l6i3f 466 13 revealing reveal VBG cord-001257-t21l6i3f 466 14 an an DT cord-001257-t21l6i3f 466 15 evolutionarily evolutionarily RB cord-001257-t21l6i3f 466 16 conserved conserved JJ cord-001257-t21l6i3f 466 17 function function NN cord-001257-t21l6i3f 466 18 outside outside IN cord-001257-t21l6i3f 466 19 the the DT cord-001257-t21l6i3f 466 20 exon exon NN cord-001257-t21l6i3f 466 21 junction junction NN cord-001257-t21l6i3f 466 22 complex complex NN cord-001257-t21l6i3f 466 23 Yeast yeast NN cord-001257-t21l6i3f 466 24 screens screen NNS cord-001257-t21l6i3f 466 25 for for IN cord-001257-t21l6i3f 466 26 host host NN cord-001257-t21l6i3f 466 27 factors factor NNS cord-001257-t21l6i3f 466 28 in in IN cord-001257-t21l6i3f 466 29 positive positive JJ cord-001257-t21l6i3f 466 30 - - HYPH cord-001257-t21l6i3f 466 31 strand strand NN cord-001257-t21l6i3f 466 32 RNA RNA NNP cord-001257-t21l6i3f 466 33 virus virus NN cord-001257-t21l6i3f 466 34 replication replication NN cord-001257-t21l6i3f 466 35 based base VBN cord-001257-t21l6i3f 466 36 on on IN cord-001257-t21l6i3f 466 37 a a DT cord-001257-t21l6i3f 466 38 library library NN cord-001257-t21l6i3f 466 39 of of IN cord-001257-t21l6i3f 466 40 temperature temperature NN cord-001257-t21l6i3f 466 41 - - HYPH cord-001257-t21l6i3f 466 42 sensitive sensitive JJ cord-001257-t21l6i3f 466 43 mutants mutant NNS cord-001257-t21l6i3f 466 44 Proteome Proteome NNP cord-001257-t21l6i3f 466 45 - - HYPH cord-001257-t21l6i3f 466 46 wide wide JJ cord-001257-t21l6i3f 466 47 overexpression overexpression NN cord-001257-t21l6i3f 466 48 of of IN cord-001257-t21l6i3f 466 49 host host NN cord-001257-t21l6i3f 466 50 proteins protein NNS cord-001257-t21l6i3f 466 51 for for IN cord-001257-t21l6i3f 466 52 identification identification NN cord-001257-t21l6i3f 466 53 of of IN cord-001257-t21l6i3f 466 54 factors factor NNS cord-001257-t21l6i3f 466 55 affecting affect VBG cord-001257-t21l6i3f 466 56 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 466 57 RNA RNA NNP cord-001257-t21l6i3f 466 58 replication replication NN cord-001257-t21l6i3f 466 59 : : : cord-001257-t21l6i3f 466 60 an an DT cord-001257-t21l6i3f 466 61 inhibitory inhibitory JJ cord-001257-t21l6i3f 466 62 role role NN cord-001257-t21l6i3f 466 63 of of IN cord-001257-t21l6i3f 466 64 protein protein NN cord-001257-t21l6i3f 466 65 kinase kinase NN cord-001257-t21l6i3f 466 66 C C NNP cord-001257-t21l6i3f 466 67 An An NNP cord-001257-t21l6i3f 466 68 internally internally RB cord-001257-t21l6i3f 466 69 located locate VBN cord-001257-t21l6i3f 466 70 RNA RNA NNP cord-001257-t21l6i3f 466 71 hairpin hairpin NN cord-001257-t21l6i3f 466 72 enhances enhance VBZ cord-001257-t21l6i3f 466 73 replication replication NN cord-001257-t21l6i3f 466 74 of of IN cord-001257-t21l6i3f 466 75 Tomato Tomato NNP cord-001257-t21l6i3f 466 76 bushy bushy JJ cord-001257-t21l6i3f 466 77 stunt stunt NN cord-001257-t21l6i3f 466 78 virus virus NN cord-001257-t21l6i3f 466 79 RNAs rna NNS cord-001257-t21l6i3f 467 1 The the DT cord-001257-t21l6i3f 467 2 RNA RNA NNP cord-001257-t21l6i3f 467 3 replication replication NN cord-001257-t21l6i3f 467 4 enhancer enhancer NN cord-001257-t21l6i3f 467 5 element element NN cord-001257-t21l6i3f 467 6 of of IN cord-001257-t21l6i3f 467 7 tombusviruses tombusviruse NNS cord-001257-t21l6i3f 467 8 contains contain VBZ cord-001257-t21l6i3f 467 9 two two CD cord-001257-t21l6i3f 467 10 interchangeable interchangeable JJ cord-001257-t21l6i3f 467 11 hairpins hairpin NNS cord-001257-t21l6i3f 467 12 that that WDT cord-001257-t21l6i3f 467 13 are be VBP cord-001257-t21l6i3f 467 14 functional functional JJ cord-001257-t21l6i3f 467 15 during during IN cord-001257-t21l6i3f 467 16 plus plus JJ cord-001257-t21l6i3f 467 17 - - HYPH cord-001257-t21l6i3f 467 18 strand strand NN cord-001257-t21l6i3f 467 19 synthesis synthesis NN cord-001257-t21l6i3f 467 20 Mechanism mechanism NN cord-001257-t21l6i3f 467 21 of of IN cord-001257-t21l6i3f 467 22 stimulation stimulation NN cord-001257-t21l6i3f 467 23 of of IN cord-001257-t21l6i3f 467 24 plus plus JJ cord-001257-t21l6i3f 467 25 - - HYPH cord-001257-t21l6i3f 467 26 strand strand NN cord-001257-t21l6i3f 467 27 synthesis synthesis NN cord-001257-t21l6i3f 467 28 by by IN cord-001257-t21l6i3f 467 29 an an DT cord-001257-t21l6i3f 467 30 RNA RNA NNP cord-001257-t21l6i3f 467 31 replication replication NN cord-001257-t21l6i3f 467 32 enhancer enhancer NN cord-001257-t21l6i3f 467 33 in in IN cord-001257-t21l6i3f 467 34 a a DT cord-001257-t21l6i3f 467 35 tombusvirus tombusvirus NN cord-001257-t21l6i3f 467 36 RNA RNA NNP cord-001257-t21l6i3f 467 37 chaperone chaperone NN cord-001257-t21l6i3f 467 38 activity activity NN cord-001257-t21l6i3f 467 39 of of IN cord-001257-t21l6i3f 467 40 the the DT cord-001257-t21l6i3f 467 41 tombusviral tombusviral JJ cord-001257-t21l6i3f 467 42 p33 p33 CD cord-001257-t21l6i3f 467 43 replication replication NN cord-001257-t21l6i3f 467 44 protein protein NN cord-001257-t21l6i3f 467 45 facilitates facilitate VBZ cord-001257-t21l6i3f 467 46 initiation initiation NN cord-001257-t21l6i3f 467 47 of of IN cord-001257-t21l6i3f 467 48 RNA RNA NNP cord-001257-t21l6i3f 467 49 synthesis synthesis NN cord-001257-t21l6i3f 467 50 by by IN cord-001257-t21l6i3f 467 51 the the DT cord-001257-t21l6i3f 467 52 viral viral JJ cord-001257-t21l6i3f 467 53 RdRp rdrp NN cord-001257-t21l6i3f 468 1 in in IN cord-001257-t21l6i3f 468 2 vitro vitro FW cord-001257-t21l6i3f 468 3 Use use NN cord-001257-t21l6i3f 468 4 of of IN cord-001257-t21l6i3f 468 5 double double RB cord-001257-t21l6i3f 468 6 - - HYPH cord-001257-t21l6i3f 468 7 stranded strand VBN cord-001257-t21l6i3f 468 8 RNA RNA NNP cord-001257-t21l6i3f 468 9 templates template NNS cord-001257-t21l6i3f 468 10 by by IN cord-001257-t21l6i3f 468 11 the the DT cord-001257-t21l6i3f 468 12 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 468 13 replicase replicase NN cord-001257-t21l6i3f 468 14 in in IN cord-001257-t21l6i3f 468 15 vitro vitro FW cord-001257-t21l6i3f 468 16 : : : cord-001257-t21l6i3f 468 17 Implications implication NNS cord-001257-t21l6i3f 468 18 for for IN cord-001257-t21l6i3f 468 19 the the DT cord-001257-t21l6i3f 468 20 mechanism mechanism NN cord-001257-t21l6i3f 468 21 of of IN cord-001257-t21l6i3f 468 22 plus plus JJ cord-001257-t21l6i3f 468 23 - - HYPH cord-001257-t21l6i3f 468 24 strand strand NN cord-001257-t21l6i3f 468 25 initiation initiation NN cord-001257-t21l6i3f 468 26 Internal internal JJ cord-001257-t21l6i3f 468 27 initiation initiation NN cord-001257-t21l6i3f 468 28 by by IN cord-001257-t21l6i3f 468 29 the the DT cord-001257-t21l6i3f 468 30 cucumber cucumber NN cord-001257-t21l6i3f 468 31 necrosis necrosis NN cord-001257-t21l6i3f 468 32 virus virus NN cord-001257-t21l6i3f 469 1 RNA RNA NNP cord-001257-t21l6i3f 469 2 - - HYPH cord-001257-t21l6i3f 469 3 dependent dependent JJ cord-001257-t21l6i3f 469 4 RNA RNA NNP cord-001257-t21l6i3f 469 5 polymerase polymerase NN cord-001257-t21l6i3f 469 6 is be VBZ cord-001257-t21l6i3f 469 7 facilitated facilitate VBN cord-001257-t21l6i3f 469 8 by by IN cord-001257-t21l6i3f 469 9 promoter promoter NN cord-001257-t21l6i3f 469 10 - - HYPH cord-001257-t21l6i3f 469 11 like like JJ cord-001257-t21l6i3f 469 12 sequences sequence NNS cord-001257-t21l6i3f 469 13 Mechanism Mechanism NNP cord-001257-t21l6i3f 469 14 of of IN cord-001257-t21l6i3f 469 15 DI DI NNP cord-001257-t21l6i3f 469 16 RNA RNA NNP cord-001257-t21l6i3f 469 17 formation formation NN cord-001257-t21l6i3f 469 18 in in IN cord-001257-t21l6i3f 469 19 tombusviruses tombusviruses NNP cord-001257-t21l6i3f 469 20 : : : cord-001257-t21l6i3f 469 21 dissecting dissect VBG cord-001257-t21l6i3f 469 22 the the DT cord-001257-t21l6i3f 469 23 requirement requirement NN cord-001257-t21l6i3f 469 24 for for IN cord-001257-t21l6i3f 469 25 primer primer NN cord-001257-t21l6i3f 469 26 extension extension NN cord-001257-t21l6i3f 469 27 by by IN cord-001257-t21l6i3f 469 28 the the DT cord-001257-t21l6i3f 469 29 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 469 30 RNA RNA NNP cord-001257-t21l6i3f 469 31 dependent dependent JJ cord-001257-t21l6i3f 469 32 RNA RNA NNP cord-001257-t21l6i3f 469 33 polymerase polymerase NN cord-001257-t21l6i3f 469 34 in in IN cord-001257-t21l6i3f 469 35 vitro vitro FW cord-001257-t21l6i3f 469 36 Partial partial JJ cord-001257-t21l6i3f 469 37 purification purification NN cord-001257-t21l6i3f 469 38 and and CC cord-001257-t21l6i3f 469 39 characterization characterization NN cord-001257-t21l6i3f 469 40 of of IN cord-001257-t21l6i3f 469 41 Cucumber Cucumber NNP cord-001257-t21l6i3f 469 42 necrosis necrosis NN cord-001257-t21l6i3f 469 43 virus virus NN cord-001257-t21l6i3f 469 44 and and CC cord-001257-t21l6i3f 469 45 Tomato Tomato NNP cord-001257-t21l6i3f 469 46 bushy bushy JJ cord-001257-t21l6i3f 469 47 stunt stunt NN cord-001257-t21l6i3f 469 48 virus virus NN cord-001257-t21l6i3f 469 49 RNA rna NN cord-001257-t21l6i3f 469 50 - - HYPH cord-001257-t21l6i3f 469 51 dependent dependent JJ cord-001257-t21l6i3f 469 52 RNA RNA NNP cord-001257-t21l6i3f 469 53 polymerases polymerase NNS cord-001257-t21l6i3f 469 54 : : : cord-001257-t21l6i3f 469 55 similarities similarity NNS cord-001257-t21l6i3f 469 56 and and CC cord-001257-t21l6i3f 469 57 differences difference NNS cord-001257-t21l6i3f 469 58 in in IN cord-001257-t21l6i3f 469 59 template template NN cord-001257-t21l6i3f 469 60 usage usage NN cord-001257-t21l6i3f 469 61 between between IN cord-001257-t21l6i3f 469 62 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 469 63 and and CC cord-001257-t21l6i3f 469 64 carmovirus carmovirus NNP cord-001257-t21l6i3f 469 65 RNA RNA NNP cord-001257-t21l6i3f 469 66 - - HYPH cord-001257-t21l6i3f 469 67 dependent dependent JJ cord-001257-t21l6i3f 469 68 RNA RNA NNP cord-001257-t21l6i3f 469 69 polymerases polymerase NNS cord-001257-t21l6i3f 469 70 Authentic authentic JJ cord-001257-t21l6i3f 469 71 replication replication NN cord-001257-t21l6i3f 469 72 and and CC cord-001257-t21l6i3f 469 73 recombination recombination NN cord-001257-t21l6i3f 469 74 of of IN cord-001257-t21l6i3f 469 75 Tomato Tomato NNP cord-001257-t21l6i3f 469 76 bushy bushy JJ cord-001257-t21l6i3f 469 77 stunt stunt NN cord-001257-t21l6i3f 469 78 virus virus NN cord-001257-t21l6i3f 469 79 RNA RNA NNP cord-001257-t21l6i3f 469 80 in in IN cord-001257-t21l6i3f 469 81 a a DT cord-001257-t21l6i3f 469 82 cell cell NN cord-001257-t21l6i3f 469 83 - - HYPH cord-001257-t21l6i3f 469 84 free free JJ cord-001257-t21l6i3f 469 85 extract extract NN cord-001257-t21l6i3f 469 86 from from IN cord-001257-t21l6i3f 469 87 yeast yeast NN cord-001257-t21l6i3f 470 1 The the DT cord-001257-t21l6i3f 470 2 p92 p92 NN cord-001257-t21l6i3f 470 3 polymerase polymerase NN cord-001257-t21l6i3f 470 4 coding code VBG cord-001257-t21l6i3f 470 5 region region NN cord-001257-t21l6i3f 470 6 contains contain VBZ cord-001257-t21l6i3f 470 7 an an DT cord-001257-t21l6i3f 470 8 internal internal JJ cord-001257-t21l6i3f 470 9 RNA RNA NNP cord-001257-t21l6i3f 470 10 element element NN cord-001257-t21l6i3f 470 11 required require VBN cord-001257-t21l6i3f 470 12 at at IN cord-001257-t21l6i3f 470 13 an an DT cord-001257-t21l6i3f 470 14 early early JJ cord-001257-t21l6i3f 470 15 step step NN cord-001257-t21l6i3f 470 16 in in IN cord-001257-t21l6i3f 470 17 Tombusvirus Tombusvirus NNP cord-001257-t21l6i3f 470 18 genome genome NN cord-001257-t21l6i3f 470 19 replication replication NN cord-001257-t21l6i3f 470 20 Composition composition NN cord-001257-t21l6i3f 470 21 of of IN cord-001257-t21l6i3f 470 22 plant plant NN cord-001257-t21l6i3f 470 23 virus virus NN cord-001257-t21l6i3f 470 24 RNA RNA NNP cord-001257-t21l6i3f 470 25 replicase replicase NNP cord-001257-t21l6i3f 470 26 complexes complexes NNP cord-001257-t21l6i3f 470 27 Global Global NNP cord-001257-t21l6i3f 470 28 organization organization NN cord-001257-t21l6i3f 470 29 of of IN cord-001257-t21l6i3f 470 30 a a DT cord-001257-t21l6i3f 470 31 positive positive JJ cord-001257-t21l6i3f 470 32 - - HYPH cord-001257-t21l6i3f 470 33 strand strand NN cord-001257-t21l6i3f 470 34 RNA RNA NNP cord-001257-t21l6i3f 470 35 virus virus NN cord-001257-t21l6i3f 470 36 genome genome NN cord-001257-t21l6i3f 470 37 Yeast Yeast NNP cord-001257-t21l6i3f 470 38 and and CC cord-001257-t21l6i3f 470 39 human human JJ cord-001257-t21l6i3f 470 40 RNA RNA NNP cord-001257-t21l6i3f 470 41 helicases helicase NNS cord-001257-t21l6i3f 470 42 involved involve VBN cord-001257-t21l6i3f 470 43 in in IN cord-001257-t21l6i3f 470 44 ribosome ribosome NN cord-001257-t21l6i3f 470 45 biogenesis biogenesis NN cord-001257-t21l6i3f 470 46 : : : cord-001257-t21l6i3f 470 47 current current JJ cord-001257-t21l6i3f 470 48 status status NN cord-001257-t21l6i3f 470 49 and and CC cord-001257-t21l6i3f 470 50 perspectives perspective NNS cord-001257-t21l6i3f 471 1 A a DT cord-001257-t21l6i3f 471 2 genome genome NN cord-001257-t21l6i3f 471 3 - - HYPH cord-001257-t21l6i3f 471 4 wide wide JJ cord-001257-t21l6i3f 471 5 analysis analysis NN cord-001257-t21l6i3f 471 6 of of IN cord-001257-t21l6i3f 471 7 the the DT cord-001257-t21l6i3f 471 8 RNA RNA NNP cord-001257-t21l6i3f 471 9 helicase helicase NN cord-001257-t21l6i3f 471 10 gene gene NN cord-001257-t21l6i3f 471 11 family family NN cord-001257-t21l6i3f 471 12 in in IN cord-001257-t21l6i3f 471 13 Solanum Solanum NNP cord-001257-t21l6i3f 471 14 lycopersicum lycopersicum NN cord-001257-t21l6i3f 471 15 Viral Viral NNP cord-001257-t21l6i3f 471 16 RNA RNA NNP cord-001257-t21l6i3f 471 17 replication replication NN cord-001257-t21l6i3f 471 18 in in IN cord-001257-t21l6i3f 471 19 association association NN cord-001257-t21l6i3f 471 20 with with IN cord-001257-t21l6i3f 471 21 cellular cellular JJ cord-001257-t21l6i3f 471 22 membranes membrane NNS cord-001257-t21l6i3f 471 23 De De NNP cord-001257-t21l6i3f 471 24 novo novo NN cord-001257-t21l6i3f 471 25 initiation initiation NN cord-001257-t21l6i3f 471 26 of of IN cord-001257-t21l6i3f 471 27 viral viral JJ cord-001257-t21l6i3f 471 28 RNA RNA NNP cord-001257-t21l6i3f 471 29 - - HYPH cord-001257-t21l6i3f 471 30 dependent dependent JJ cord-001257-t21l6i3f 471 31 RNA RNA NNP cord-001257-t21l6i3f 471 32 synthesis synthesis NN cord-001257-t21l6i3f 471 33 Filling fill VBG cord-001257-t21l6i3f 471 34 a a DT cord-001257-t21l6i3f 471 35 GAP(DH gap(dh CD cord-001257-t21l6i3f 471 36 ) ) -RRB- cord-001257-t21l6i3f 471 37 in in IN cord-001257-t21l6i3f 471 38 asymmetric asymmetric JJ cord-001257-t21l6i3f 471 39 viral viral JJ cord-001257-t21l6i3f 471 40 RNA RNA NNP cord-001257-t21l6i3f 471 41 synthesis synthesis NN cord-001257-t21l6i3f 471 42 Characterization characterization NN cord-001257-t21l6i3f 471 43 of of IN cord-001257-t21l6i3f 471 44 the the DT cord-001257-t21l6i3f 471 45 RNA RNA NNP cord-001257-t21l6i3f 471 46 - - HYPH cord-001257-t21l6i3f 471 47 binding bind VBG cord-001257-t21l6i3f 471 48 domains domain NNS cord-001257-t21l6i3f 471 49 in in IN cord-001257-t21l6i3f 471 50 the the DT cord-001257-t21l6i3f 471 51 replicase replicase NN cord-001257-t21l6i3f 471 52 proteins protein NNS cord-001257-t21l6i3f 471 53 of of IN cord-001257-t21l6i3f 471 54 tomato tomato NN cord-001257-t21l6i3f 471 55 bushy bushy JJ cord-001257-t21l6i3f 471 56 stunt stunt NN cord-001257-t21l6i3f 471 57 virus virus NN cord-001257-t21l6i3f 471 58 Direct direct JJ cord-001257-t21l6i3f 471 59 inhibition inhibition NN cord-001257-t21l6i3f 471 60 of of IN cord-001257-t21l6i3f 471 61 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 471 62 plus plus CC cord-001257-t21l6i3f 471 63 - - HYPH cord-001257-t21l6i3f 471 64 strand strand NN cord-001257-t21l6i3f 471 65 RNA RNA NNP cord-001257-t21l6i3f 471 66 synthesis synthesis NN cord-001257-t21l6i3f 471 67 by by IN cord-001257-t21l6i3f 471 68 a a DT cord-001257-t21l6i3f 471 69 dominant dominant JJ cord-001257-t21l6i3f 471 70 - - HYPH cord-001257-t21l6i3f 471 71 negative negative JJ cord-001257-t21l6i3f 471 72 mutant mutant NN cord-001257-t21l6i3f 471 73 of of IN cord-001257-t21l6i3f 471 74 a a DT cord-001257-t21l6i3f 471 75 host host NN cord-001257-t21l6i3f 471 76 metabolic metabolic JJ cord-001257-t21l6i3f 471 77 enzyme enzyme NN cord-001257-t21l6i3f 471 78 , , , cord-001257-t21l6i3f 471 79 GAPDH GAPDH NNP cord-001257-t21l6i3f 471 80 , , , cord-001257-t21l6i3f 471 81 in in IN cord-001257-t21l6i3f 471 82 yeast yeast NN cord-001257-t21l6i3f 471 83 and and CC cord-001257-t21l6i3f 471 84 plants plant NNS cord-001257-t21l6i3f 472 1 Distinct distinct JJ cord-001257-t21l6i3f 472 2 DDX DDX NNP cord-001257-t21l6i3f 472 3 DEAD DEAD NNP cord-001257-t21l6i3f 472 4 - - HYPH cord-001257-t21l6i3f 472 5 box box NN cord-001257-t21l6i3f 472 6 RNA RNA NNP cord-001257-t21l6i3f 472 7 helicases helicase NNS cord-001257-t21l6i3f 472 8 cooperate cooperate VBP cord-001257-t21l6i3f 472 9 to to TO cord-001257-t21l6i3f 472 10 modulate modulate VB cord-001257-t21l6i3f 472 11 the the DT cord-001257-t21l6i3f 472 12 HIV-1 HIV-1 NNP cord-001257-t21l6i3f 472 13 Rev Rev NNP cord-001257-t21l6i3f 472 14 function function NN cord-001257-t21l6i3f 472 15 From from IN cord-001257-t21l6i3f 472 16 promoting promote VBG cord-001257-t21l6i3f 472 17 to to IN cord-001257-t21l6i3f 472 18 inhibiting inhibit VBG cord-001257-t21l6i3f 472 19 : : : cord-001257-t21l6i3f 472 20 diverse diverse JJ cord-001257-t21l6i3f 472 21 roles role NNS cord-001257-t21l6i3f 472 22 of of IN cord-001257-t21l6i3f 472 23 helicases helicase NNS cord-001257-t21l6i3f 472 24 in in IN cord-001257-t21l6i3f 472 25 HIV-1 HIV-1 NNP cord-001257-t21l6i3f 472 26 Replication Replication NNP cord-001257-t21l6i3f 472 27 Viruses Viruses NNPS cord-001257-t21l6i3f 472 28 and and CC cord-001257-t21l6i3f 472 29 the the DT cord-001257-t21l6i3f 472 30 human human JJ cord-001257-t21l6i3f 472 31 DEAD DEAD NNP cord-001257-t21l6i3f 472 32 - - HYPH cord-001257-t21l6i3f 472 33 box box NN cord-001257-t21l6i3f 472 34 helicase helicase NN cord-001257-t21l6i3f 472 35 DDX3 DDX3 NNP cord-001257-t21l6i3f 472 36 : : : cord-001257-t21l6i3f 472 37 inhibition inhibition NN cord-001257-t21l6i3f 472 38 or or CC cord-001257-t21l6i3f 472 39 exploitation exploitation NN cord-001257-t21l6i3f 472 40 ? ? . cord-001257-t21l6i3f 473 1 A a DT cord-001257-t21l6i3f 473 2 mutant mutant JJ cord-001257-t21l6i3f 473 3 allele allele NN cord-001257-t21l6i3f 473 4 of of IN cord-001257-t21l6i3f 473 5 essential essential JJ cord-001257-t21l6i3f 473 6 , , , cord-001257-t21l6i3f 473 7 general general JJ cord-001257-t21l6i3f 473 8 translation translation NN cord-001257-t21l6i3f 473 9 initiation initiation NN cord-001257-t21l6i3f 473 10 factor factor NN cord-001257-t21l6i3f 473 11 DED1 DED1 NNP cord-001257-t21l6i3f 473 12 selectively selectively RB cord-001257-t21l6i3f 473 13 inhibits inhibit VBZ cord-001257-t21l6i3f 473 14 translation translation NN cord-001257-t21l6i3f 473 15 of of IN cord-001257-t21l6i3f 473 16 a a DT cord-001257-t21l6i3f 473 17 viral viral JJ cord-001257-t21l6i3f 473 18 mRNA mRNA NNP cord-001257-t21l6i3f 473 19 RNA RNA NNP cord-001257-t21l6i3f 473 20 helicase helicase NN cord-001257-t21l6i3f 474 1 A a DT cord-001257-t21l6i3f 474 2 modulates modulate VBZ cord-001257-t21l6i3f 474 3 translation translation NN cord-001257-t21l6i3f 474 4 of of IN cord-001257-t21l6i3f 474 5 HIV-1 HIV-1 NNP cord-001257-t21l6i3f 474 6 and and CC cord-001257-t21l6i3f 474 7 infectivity infectivity NN cord-001257-t21l6i3f 474 8 of of IN cord-001257-t21l6i3f 474 9 progeny progeny JJ cord-001257-t21l6i3f 474 10 virions virion NNS cord-001257-t21l6i3f 475 1 Autogenous autogenous JJ cord-001257-t21l6i3f 475 2 translational translational JJ cord-001257-t21l6i3f 475 3 regulation regulation NN cord-001257-t21l6i3f 475 4 of of IN cord-001257-t21l6i3f 475 5 the the DT cord-001257-t21l6i3f 475 6 Borna Borna NNP cord-001257-t21l6i3f 475 7 disease disease NN cord-001257-t21l6i3f 475 8 virus virus NN cord-001257-t21l6i3f 475 9 negative negative JJ cord-001257-t21l6i3f 475 10 control control NN cord-001257-t21l6i3f 475 11 factor factor NN cord-001257-t21l6i3f 475 12 X X NNP cord-001257-t21l6i3f 475 13 from from IN cord-001257-t21l6i3f 475 14 polycistronic polycistronic NNP cord-001257-t21l6i3f 475 15 mRNA mRNA NNP cord-001257-t21l6i3f 475 16 using use VBG cord-001257-t21l6i3f 475 17 host host NN cord-001257-t21l6i3f 475 18 RNA RNA NNP cord-001257-t21l6i3f 475 19 helicases helicase NNS cord-001257-t21l6i3f 475 20 Cyclosporin cyclosporin XX cord-001257-t21l6i3f 476 1 a a DT cord-001257-t21l6i3f 476 2 associated associated JJ cord-001257-t21l6i3f 476 3 helicase helicase NN cord-001257-t21l6i3f 476 4 - - HYPH cord-001257-t21l6i3f 476 5 like like JJ cord-001257-t21l6i3f 476 6 protein protein NN cord-001257-t21l6i3f 476 7 facilitates facilitate VBZ cord-001257-t21l6i3f 476 8 the the DT cord-001257-t21l6i3f 476 9 association association NN cord-001257-t21l6i3f 476 10 of of IN cord-001257-t21l6i3f 476 11 hepatitis hepatitis NNP cord-001257-t21l6i3f 476 12 C C NNP cord-001257-t21l6i3f 476 13 virus virus NN cord-001257-t21l6i3f 476 14 RNA rna NN cord-001257-t21l6i3f 476 15 polymerase polymerase NN cord-001257-t21l6i3f 476 16 with with IN cord-001257-t21l6i3f 476 17 its -PRON- PRP$ cord-001257-t21l6i3f 476 18 cellular cellular JJ cord-001257-t21l6i3f 476 19 cyclophilin cyclophilin NN cord-001257-t21l6i3f 476 20 B B NNP cord-001257-t21l6i3f 476 21 Identification Identification NNP cord-001257-t21l6i3f 476 22 of of IN cord-001257-t21l6i3f 476 23 RNA RNA NNP cord-001257-t21l6i3f 476 24 helicase helicase NNP cord-001257-t21l6i3f 476 25 A a NN cord-001257-t21l6i3f 476 26 as as IN cord-001257-t21l6i3f 476 27 a a DT cord-001257-t21l6i3f 476 28 new new JJ cord-001257-t21l6i3f 476 29 host host NN cord-001257-t21l6i3f 476 30 factor factor NN cord-001257-t21l6i3f 476 31 in in IN cord-001257-t21l6i3f 476 32 the the DT cord-001257-t21l6i3f 476 33 replication replication NN cord-001257-t21l6i3f 476 34 cycle cycle NN cord-001257-t21l6i3f 476 35 of of IN cord-001257-t21l6i3f 476 36 foot foot NN cord-001257-t21l6i3f 476 37 - - HYPH cord-001257-t21l6i3f 476 38 and and CC cord-001257-t21l6i3f 476 39 - - HYPH cord-001257-t21l6i3f 476 40 mouth mouth NN cord-001257-t21l6i3f 476 41 disease disease NN cord-001257-t21l6i3f 476 42 virus virus NN cord-001257-t21l6i3f 476 43 Cellular Cellular NNP cord-001257-t21l6i3f 476 44 RNA RNA NNP cord-001257-t21l6i3f 476 45 helicase helicase NN cord-001257-t21l6i3f 476 46 p68 p68 NNP cord-001257-t21l6i3f 476 47 relocalization relocalization NN cord-001257-t21l6i3f 476 48 and and CC cord-001257-t21l6i3f 476 49 interaction interaction NN cord-001257-t21l6i3f 476 50 with with IN cord-001257-t21l6i3f 476 51 the the DT cord-001257-t21l6i3f 476 52 hepatitis hepatitis NN cord-001257-t21l6i3f 476 53 C C NNP cord-001257-t21l6i3f 476 54 virus virus NN cord-001257-t21l6i3f 476 55 ( ( -LRB- cord-001257-t21l6i3f 476 56 HCV HCV NNP cord-001257-t21l6i3f 476 57 ) ) -RRB- cord-001257-t21l6i3f 476 58 NS5B NS5B NNP cord-001257-t21l6i3f 476 59 protein protein NN cord-001257-t21l6i3f 476 60 and and CC cord-001257-t21l6i3f 476 61 the the DT cord-001257-t21l6i3f 476 62 potential potential JJ cord-001257-t21l6i3f 476 63 role role NN cord-001257-t21l6i3f 476 64 of of IN cord-001257-t21l6i3f 476 65 p68 p68 NN cord-001257-t21l6i3f 476 66 in in IN cord-001257-t21l6i3f 477 1 HCV HCV NNP cord-001257-t21l6i3f 477 2 RNA RNA NNP cord-001257-t21l6i3f 477 3 replication replication NN cord-001257-t21l6i3f 477 4 Affinity affinity NN cord-001257-t21l6i3f 477 5 capture capture NN cord-001257-t21l6i3f 477 6 and and CC cord-001257-t21l6i3f 477 7 identification identification NN cord-001257-t21l6i3f 477 8 of of IN cord-001257-t21l6i3f 477 9 host host NN cord-001257-t21l6i3f 477 10 cell cell NN cord-001257-t21l6i3f 477 11 factors factor NNS cord-001257-t21l6i3f 477 12 associated associate VBN cord-001257-t21l6i3f 477 13 with with IN cord-001257-t21l6i3f 477 14 hepatitis hepatitis NN cord-001257-t21l6i3f 477 15 C C NNP cord-001257-t21l6i3f 477 16 virus virus NN cord-001257-t21l6i3f 477 17 ( ( -LRB- cord-001257-t21l6i3f 477 18 + + CC cord-001257-t21l6i3f 478 1 ) ) -RRB- cord-001257-t21l6i3f 478 2 strand strand NN cord-001257-t21l6i3f 478 3 subgenomic subgenomic NNP cord-001257-t21l6i3f 478 4 RNA RNA NNP cord-001257-t21l6i3f 479 1 The the DT cord-001257-t21l6i3f 479 2 cellular cellular JJ cord-001257-t21l6i3f 479 3 decapping decapping NN cord-001257-t21l6i3f 479 4 activators activator NNS cord-001257-t21l6i3f 479 5 LSm1 lsm1 RB cord-001257-t21l6i3f 479 6 , , , cord-001257-t21l6i3f 479 7 Pat1 Pat1 NNP cord-001257-t21l6i3f 479 8 , , , cord-001257-t21l6i3f 479 9 and and CC cord-001257-t21l6i3f 479 10 Dhh1 Dhh1 NNP cord-001257-t21l6i3f 479 11 control control VB cord-001257-t21l6i3f 479 12 the the DT cord-001257-t21l6i3f 479 13 ratio ratio NN cord-001257-t21l6i3f 479 14 of of IN cord-001257-t21l6i3f 479 15 subgenomic subgenomic NNP cord-001257-t21l6i3f 479 16 to to TO cord-001257-t21l6i3f 479 17 genomic genomic VB cord-001257-t21l6i3f 479 18 Flock Flock NNP cord-001257-t21l6i3f 479 19 House House NNP cord-001257-t21l6i3f 479 20 virus virus NN cord-001257-t21l6i3f 479 21 RNAs RNAs NNP cord-001257-t21l6i3f 479 22 DDX3 DDX3 NNP cord-001257-t21l6i3f 479 23 DEAD DEAD NNP cord-001257-t21l6i3f 479 24 - - HYPH cord-001257-t21l6i3f 479 25 Box Box NNP cord-001257-t21l6i3f 479 26 RNA RNA NNP cord-001257-t21l6i3f 479 27 helicase helicase NN cord-001257-t21l6i3f 480 1 inhibits inhibit NNS cord-001257-t21l6i3f 481 1 hepatitis hepatitis NNP cord-001257-t21l6i3f 481 2 B B NNP cord-001257-t21l6i3f 481 3 virus virus NN cord-001257-t21l6i3f 481 4 reverse reverse VB cord-001257-t21l6i3f 481 5 transcription transcription NN cord-001257-t21l6i3f 481 6 by by IN cord-001257-t21l6i3f 481 7 incorporation incorporation NN cord-001257-t21l6i3f 481 8 into into IN cord-001257-t21l6i3f 481 9 nucleocapsids nucleocapsid NNS cord-001257-t21l6i3f 482 1 The the DT cord-001257-t21l6i3f 482 2 helicase helicase NN cord-001257-t21l6i3f 482 3 activity activity NN cord-001257-t21l6i3f 482 4 of of IN cord-001257-t21l6i3f 482 5 DDX56 DDX56 NNP cord-001257-t21l6i3f 482 6 is be VBZ cord-001257-t21l6i3f 482 7 required require VBN cord-001257-t21l6i3f 482 8 for for IN cord-001257-t21l6i3f 482 9 its -PRON- PRP$ cord-001257-t21l6i3f 482 10 role role NN cord-001257-t21l6i3f 482 11 in in IN cord-001257-t21l6i3f 482 12 assembly assembly NN cord-001257-t21l6i3f 482 13 of of IN cord-001257-t21l6i3f 482 14 infectious infectious JJ cord-001257-t21l6i3f 482 15 West West NNP cord-001257-t21l6i3f 482 16 Nile Nile NNP cord-001257-t21l6i3f 482 17 virus virus NN cord-001257-t21l6i3f 482 18 particles particle NNS cord-001257-t21l6i3f 483 1 Kaposi Kaposi NNP cord-001257-t21l6i3f 483 2 's 's POS cord-001257-t21l6i3f 483 3 sarcoma sarcoma NN cord-001257-t21l6i3f 483 4 - - HYPH cord-001257-t21l6i3f 483 5 associated associate VBN cord-001257-t21l6i3f 483 6 herpesvirus herpesvirus NN cord-001257-t21l6i3f 483 7 viral viral JJ cord-001257-t21l6i3f 483 8 protein protein NN cord-001257-t21l6i3f 483 9 kinase kinase NN cord-001257-t21l6i3f 483 10 interacts interact VBZ cord-001257-t21l6i3f 483 11 with with IN cord-001257-t21l6i3f 483 12 RNA RNA NNP cord-001257-t21l6i3f 483 13 helicase helicase NNP cord-001257-t21l6i3f 483 14 a a DT cord-001257-t21l6i3f 483 15 and and CC cord-001257-t21l6i3f 483 16 regulates regulate VBZ cord-001257-t21l6i3f 483 17 host host NN cord-001257-t21l6i3f 483 18 gene gene NN cord-001257-t21l6i3f 483 19 expression expression NN cord-001257-t21l6i3f 484 1 DEXH DEXH NNP cord-001257-t21l6i3f 484 2 - - HYPH cord-001257-t21l6i3f 484 3 Box Box NNP cord-001257-t21l6i3f 484 4 protein protein NN cord-001257-t21l6i3f 484 5 DHX30 DHX30 NNP cord-001257-t21l6i3f 484 6 is be VBZ cord-001257-t21l6i3f 484 7 required require VBN cord-001257-t21l6i3f 484 8 for for IN cord-001257-t21l6i3f 484 9 optimal optimal JJ cord-001257-t21l6i3f 484 10 function function NN cord-001257-t21l6i3f 484 11 of of IN cord-001257-t21l6i3f 484 12 the the DT cord-001257-t21l6i3f 484 13 zinc zinc NN cord-001257-t21l6i3f 484 14 - - HYPH cord-001257-t21l6i3f 484 15 finger finger NN cord-001257-t21l6i3f 484 16 antiviral antiviral JJ cord-001257-t21l6i3f 484 17 protein protein NN cord-001257-t21l6i3f 484 18 Regulating regulate VBG cord-001257-t21l6i3f 484 19 intracellular intracellular JJ cord-001257-t21l6i3f 484 20 antiviral antiviral JJ cord-001257-t21l6i3f 484 21 defense defense NN cord-001257-t21l6i3f 484 22 and and CC cord-001257-t21l6i3f 484 23 permissiveness permissiveness NN cord-001257-t21l6i3f 484 24 to to IN cord-001257-t21l6i3f 484 25 hepatitis hepatitis NNP cord-001257-t21l6i3f 484 26 C C NNP cord-001257-t21l6i3f 484 27 virus virus NN cord-001257-t21l6i3f 484 28 RNA RNA NNP cord-001257-t21l6i3f 484 29 replication replication NN cord-001257-t21l6i3f 484 30 through through IN cord-001257-t21l6i3f 484 31 a a DT cord-001257-t21l6i3f 484 32 cellular cellular JJ cord-001257-t21l6i3f 484 33 RNA rna NN cord-001257-t21l6i3f 484 34 helicase helicase NN cord-001257-t21l6i3f 484 35 , , , cord-001257-t21l6i3f 484 36 RIG rig NN cord-001257-t21l6i3f 484 37 - - : cord-001257-t21l6i3f 484 38 I -PRON- PRP cord-001257-t21l6i3f 484 39 DExD DExD NNS cord-001257-t21l6i3f 484 40 / / SYM cord-001257-t21l6i3f 484 41 H h NN cord-001257-t21l6i3f 484 42 - - HYPH cord-001257-t21l6i3f 484 43 box box NN cord-001257-t21l6i3f 484 44 RNA RNA NNP cord-001257-t21l6i3f 484 45 helicases helicase NNS cord-001257-t21l6i3f 484 46 as as IN cord-001257-t21l6i3f 484 47 mediators mediator NNS cord-001257-t21l6i3f 484 48 of of IN cord-001257-t21l6i3f 484 49 antiviral antiviral JJ cord-001257-t21l6i3f 484 50 innate innate JJ cord-001257-t21l6i3f 484 51 immunity immunity NN cord-001257-t21l6i3f 484 52 and and CC cord-001257-t21l6i3f 484 53 essential essential JJ cord-001257-t21l6i3f 484 54 host host NN cord-001257-t21l6i3f 484 55 factors factor NNS cord-001257-t21l6i3f 484 56 for for IN cord-001257-t21l6i3f 484 57 viral viral JJ cord-001257-t21l6i3f 484 58 replication replication NN cord-001257-t21l6i3f 484 59 Systematic systematic JJ cord-001257-t21l6i3f 484 60 exploration exploration NN cord-001257-t21l6i3f 484 61 of of IN cord-001257-t21l6i3f 484 62 essential essential JJ cord-001257-t21l6i3f 484 63 yeast yeast NN cord-001257-t21l6i3f 484 64 gene gene NN cord-001257-t21l6i3f 484 65 function function NN cord-001257-t21l6i3f 484 66 with with IN cord-001257-t21l6i3f 484 67 temperature temperature NN cord-001257-t21l6i3f 484 68 - - HYPH cord-001257-t21l6i3f 484 69 sensitive sensitive JJ cord-001257-t21l6i3f 484 70 mutants mutant NNS cord-001257-t21l6i3f 485 1 A a DT cord-001257-t21l6i3f 485 2 versatile versatile JJ cord-001257-t21l6i3f 485 3 toolbox toolbox NN cord-001257-t21l6i3f 485 4 for for IN cord-001257-t21l6i3f 485 5 PCR PCR NNP cord-001257-t21l6i3f 485 6 - - HYPH cord-001257-t21l6i3f 485 7 based base VBN cord-001257-t21l6i3f 485 8 tagging tagging NN cord-001257-t21l6i3f 485 9 of of IN cord-001257-t21l6i3f 485 10 yeast yeast NN cord-001257-t21l6i3f 485 11 genes gene NNS cord-001257-t21l6i3f 485 12 : : : cord-001257-t21l6i3f 485 13 new new JJ cord-001257-t21l6i3f 485 14 fluorescent fluorescent JJ cord-001257-t21l6i3f 485 15 proteins protein NNS cord-001257-t21l6i3f 485 16 , , , cord-001257-t21l6i3f 485 17 more more JJR cord-001257-t21l6i3f 485 18 markers marker NNS cord-001257-t21l6i3f 485 19 and and CC cord-001257-t21l6i3f 485 20 promoter promoter NN cord-001257-t21l6i3f 485 21 substitution substitution NN cord-001257-t21l6i3f 485 22 cassettes cassette NNS cord-001257-t21l6i3f 486 1 Cyclophilin Cyclophilin NNP cord-001257-t21l6i3f 486 2 A A NNP cord-001257-t21l6i3f 486 3 binds bind VBZ cord-001257-t21l6i3f 486 4 to to IN cord-001257-t21l6i3f 486 5 the the DT cord-001257-t21l6i3f 486 6 viral viral JJ cord-001257-t21l6i3f 486 7 RNA rna NN cord-001257-t21l6i3f 486 8 and and CC cord-001257-t21l6i3f 486 9 replication replication NN cord-001257-t21l6i3f 486 10 proteins protein NNS cord-001257-t21l6i3f 486 11 resulting result VBG cord-001257-t21l6i3f 486 12 in in IN cord-001257-t21l6i3f 486 13 inhibition inhibition NN cord-001257-t21l6i3f 486 14 of of IN cord-001257-t21l6i3f 486 15 tombusviral tombusviral JJ cord-001257-t21l6i3f 486 16 replicase replicase NNP cord-001257-t21l6i3f 486 17 assembly assembly NNP cord-001257-t21l6i3f 487 1 The the DT cord-001257-t21l6i3f 487 2 Nedd4-type Nedd4-type NNP cord-001257-t21l6i3f 487 3 Rsp5p rsp5p NN cord-001257-t21l6i3f 487 4 ubiquitin ubiquitin NN cord-001257-t21l6i3f 487 5 ligase ligase NN cord-001257-t21l6i3f 487 6 inhibits inhibit VBZ cord-001257-t21l6i3f 487 7 tombusvirus tombusvirus NN cord-001257-t21l6i3f 487 8 replication replication NN cord-001257-t21l6i3f 487 9 by by IN cord-001257-t21l6i3f 487 10 regulating regulate VBG cord-001257-t21l6i3f 487 11 degradation degradation NN cord-001257-t21l6i3f 487 12 of of IN cord-001257-t21l6i3f 487 13 the the DT cord-001257-t21l6i3f 487 14 p92 p92 NN cord-001257-t21l6i3f 487 15 replication replication NN cord-001257-t21l6i3f 487 16 protein protein NN cord-001257-t21l6i3f 487 17 and and CC cord-001257-t21l6i3f 487 18 decreasing decrease VBG cord-001257-t21l6i3f 487 19 the the DT cord-001257-t21l6i3f 487 20 activity activity NN cord-001257-t21l6i3f 487 21 of of IN cord-001257-t21l6i3f 487 22 the the DT cord-001257-t21l6i3f 487 23 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 487 24 replicase replicase NNP cord-001257-t21l6i3f 487 25 Expression Expression NNP cord-001257-t21l6i3f 487 26 of of IN cord-001257-t21l6i3f 487 27 the the DT cord-001257-t21l6i3f 487 28 Arabidopsis Arabidopsis NNP cord-001257-t21l6i3f 487 29 Xrn4p Xrn4p NNP cord-001257-t21l6i3f 487 30 59 59 CD cord-001257-t21l6i3f 487 31 - - SYM cord-001257-t21l6i3f 487 32 39 39 CD cord-001257-t21l6i3f 487 33 exoribonuclease exoribonuclease NN cord-001257-t21l6i3f 487 34 facilitates facilitate VBZ cord-001257-t21l6i3f 487 35 degradation degradation NN cord-001257-t21l6i3f 487 36 of of IN cord-001257-t21l6i3f 487 37 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 487 38 RNA RNA NNP cord-001257-t21l6i3f 487 39 and and CC cord-001257-t21l6i3f 487 40 promotes promote VBZ cord-001257-t21l6i3f 487 41 rapid rapid JJ cord-001257-t21l6i3f 487 42 emergence emergence NN cord-001257-t21l6i3f 487 43 of of IN cord-001257-t21l6i3f 487 44 viral viral JJ cord-001257-t21l6i3f 487 45 variants variant NNS cord-001257-t21l6i3f 487 46 in in IN cord-001257-t21l6i3f 487 47 plants plant NNS cord-001257-t21l6i3f 487 48 Comparison comparison NN cord-001257-t21l6i3f 487 49 of of IN cord-001257-t21l6i3f 487 50 turnip turnip NN cord-001257-t21l6i3f 487 51 crinkle crinkle NN cord-001257-t21l6i3f 487 52 virus virus NN cord-001257-t21l6i3f 487 53 RNA rna NN cord-001257-t21l6i3f 487 54 - - HYPH cord-001257-t21l6i3f 487 55 dependent dependent JJ cord-001257-t21l6i3f 487 56 RNA RNA NNP cord-001257-t21l6i3f 487 57 polymerase polymerase NN cord-001257-t21l6i3f 487 58 preparations preparation NNS cord-001257-t21l6i3f 487 59 expressed express VBN cord-001257-t21l6i3f 487 60 in in IN cord-001257-t21l6i3f 487 61 Escherichia Escherichia NNP cord-001257-t21l6i3f 487 62 coli coli NNS cord-001257-t21l6i3f 487 63 or or CC cord-001257-t21l6i3f 487 64 derived derive VBN cord-001257-t21l6i3f 487 65 from from IN cord-001257-t21l6i3f 487 66 infected infected JJ cord-001257-t21l6i3f 487 67 plants plant NNS cord-001257-t21l6i3f 487 68 Analysis Analysis NNP cord-001257-t21l6i3f 487 69 of of IN cord-001257-t21l6i3f 487 70 minimal minimal JJ cord-001257-t21l6i3f 487 71 promoter promoter NN cord-001257-t21l6i3f 487 72 sequences sequence NNS cord-001257-t21l6i3f 487 73 for for IN cord-001257-t21l6i3f 487 74 plus plus JJ cord-001257-t21l6i3f 487 75 - - HYPH cord-001257-t21l6i3f 487 76 strand strand NN cord-001257-t21l6i3f 487 77 synthesis synthesis NN cord-001257-t21l6i3f 487 78 by by IN cord-001257-t21l6i3f 487 79 the the DT cord-001257-t21l6i3f 487 80 Cucumber Cucumber NNP cord-001257-t21l6i3f 487 81 necrosis necrosis NN cord-001257-t21l6i3f 487 82 virus virus NN cord-001257-t21l6i3f 487 83 RNA rna NN cord-001257-t21l6i3f 487 84 - - HYPH cord-001257-t21l6i3f 487 85 dependent dependent JJ cord-001257-t21l6i3f 487 86 RNA RNA NNP cord-001257-t21l6i3f 487 87 polymerase polymerase NN cord-001257-t21l6i3f 488 1 Nucleolin Nucleolin NNP cord-001257-t21l6i3f 488 2 / / SYM cord-001257-t21l6i3f 488 3 Nsr1p Nsr1p NNP cord-001257-t21l6i3f 488 4 binds bind VBZ cord-001257-t21l6i3f 488 5 to to IN cord-001257-t21l6i3f 488 6 the the DT cord-001257-t21l6i3f 488 7 39 39 CD cord-001257-t21l6i3f 488 8 noncoding noncoding JJ cord-001257-t21l6i3f 488 9 region region NN cord-001257-t21l6i3f 488 10 of of IN cord-001257-t21l6i3f 488 11 the the DT cord-001257-t21l6i3f 488 12 tombusvirus tombusvirus NNP cord-001257-t21l6i3f 488 13 RNA RNA NNP cord-001257-t21l6i3f 488 14 and and CC cord-001257-t21l6i3f 488 15 inhibits inhibit VBZ cord-001257-t21l6i3f 488 16 replication replication NN cord-001257-t21l6i3f 489 1 The the DT cord-001257-t21l6i3f 489 2 authors author NNS cord-001257-t21l6i3f 489 3 thank thank VBP cord-001257-t21l6i3f 489 4 Drs Drs NNP cord-001257-t21l6i3f 489 5 . . . cord-001257-t21l6i3f 490 1 Ching Ching NNP cord-001257-t21l6i3f 490 2 - - HYPH cord-001257-t21l6i3f 490 3 Kai Kai NNP cord-001257-t21l6i3f 490 4 Chuang Chuang NNP cord-001257-t21l6i3f 490 5 and and CC cord-001257-t21l6i3f 490 6 Judit Judit NNP cord-001257-t21l6i3f 490 7 Pogany Pogany NNP cord-001257-t21l6i3f 490 8 for for IN cord-001257-t21l6i3f 490 9 valuable valuable JJ cord-001257-t21l6i3f 490 10 comments comment NNS cord-001257-t21l6i3f 490 11 . . . cord-001257-t21l6i3f 490 12 _SP