id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-325479-2r4oomdp	Torii, Shotaro	Applicability of polyethylene glycol precipitation followed by acid guanidinium thiocyanate-phenol-chloroform extraction for the detection of SARS-CoV-2 RNA from municipal wastewater	2020-10-17		.txt	text/plain	5881	363	58	This study aims (1) to compare the whole process recovery of Pseudomonas phage φ6, a surrogate for enveloped viruses, among combinations of primary concentration [ultrafiltration (UF), electronegative membrane vortex (EMV), and polyethylene glycol precipitation (PEG)] and RNA extraction methods (spin column-based method using QIAamp Viral RNA Mini Kit and acid guanidinium thiocyanate–phenol–chloroform extraction using TRIzol reagent) for three types of raw sewage and (2) to test the applicability of the method providing the highest φ6 recovery to the detection of SARS-CoV-2 RNA. This study aims (1) to compare the combination of primary concentration (UF, EMV, and PEG) and RNA extraction (QIAamp Viral RNA Mini Kit and TRIzol) for the whole process recovery of nonenveloped and enveloped virus surrogates and (2) to test the applicability of the method providing the highest φ6 recovery to detect SARS-CoV-2	./cache/cord-325479-2r4oomdp.txt	./txt/cord-325479-2r4oomdp.txt