id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-263862-zzys31e9	Ryan, Elizabeth J.	The Canarypox-virus vaccine vector ALVAC triggers the release of IFN-γ by Natural Killer (NK) cells enhancing Th1 polarization	2007-04-30		.txt	text/plain	5843	318	57	Therefore, this leads us to suggest that ALVAC can act as a Th1 polarizing adjuvant by inducing local inflammation [7] , resulting in DC maturation and chemokine production which in turn causes the recruitment of IFN-␥ secreting NK cells. Understanding the mechanism of adjuvant action of ALVAC, will allow more effective targeting of the desired immune response by taking advantage of the fact that ALVAC can be manipulated to encode a variety of immunomodulatory transgenes, e.g. GM-CSF [11] or IL-12 [12] that can further enhance the activation of NK cells or DCs. Six to eight week old female Balb/c ByJ mice (Charles River, Les Oncins, France) or 129/Sv, IFN-␣ receptor knockout and IFN-␥ receptor knockout (B + K Universal, Hull, UK) were used. In order to detect circulating IFN-␣ groups of Balb/c mice were immunised i.m. with 1/10th of the human dose of ALVAC and then bled at various time-points, and the levels of IFN-␣ in the serum were determined by ELISA.	./cache/cord-263862-zzys31e9.txt	./txt/cord-263862-zzys31e9.txt