id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-302265-97sxlkjp	Ramasamy, R.	Immunogenicity of a malaria parasite antigen displayed by Lactococcus lactis in oral immunisations	2006-05-01		.txt	text/plain	5386	267	47	A putative protective protein from Plasmodium falciparum merozoites, MSA2, was expressed in two different ways on the cell surface of the Gram-positive food-grade bacterium, Lactococcus lactis. In a second display format, MSA2 was fused to the peptidoglycan-binding domain (Protein Anchor) of the lactococcal cell wall hydrolase AcmA and was non-covalently rebound to the surface of non-genetically modified, non-living high-binder L. lactis recombinants carrying covalently bound MSA2 were used to immunise rabbits through nasal and oral routes. lactis-pNG3043 for immunisation were prepared from overnight cultures (the latter strain was induced overnight with nisin A for MSA2-Cov expression) and stored in aliquots of 10 11 colony forming units (cfu) per ml growth medium containing 10% glycerol at −80 • C. lactis-pNG3041(MSA2-Cov) or 5 × 10 10 GEM particles with bound MSA2-nCov. Each dose was repeated for three successive days to obtain reproducible oral immunisation. lactis response was even more minimized in the rabbit orally immunised with the GEM particle vaccine (Fig. 5C ).	./cache/cord-302265-97sxlkjp.txt	./txt/cord-302265-97sxlkjp.txt