id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-004400-li1sc47z	Ma, Jingjiao	Acetylation at K108 of the NS1 protein is important for the replication and virulence of influenza virus	2020-02-24		.txt	text/plain	4836	225	49	However, the growth of the deacetylated mutant virus WSN-NS1-108R was significantly impaired compared with that of the other two viruses in MDCK and A549 cells at 36 and 48 hpi, which indicated that the acetylated K108 of NS1 is important for virus replication in vitro at the late stage of infection (Figures 2A and B) . To further explore how the K108R mutation attenuated the IFN-β antagonistic ability of the NS1 protein, we co-transfected NS1 expression plasmids, an IFN-β reporter plasmid and different type I interferon pathway components, including RIG-I CARD, TBK1, and the active form of IRF3, into 293T cells. This result indicated that the deacetylation-mimic K108R substitution retained NS1 protein in the cytoplasm of infected cells, suggesting that the acetylated K108 residue is important for the nuclear localization of the NS1 protein ( Figures 5A-C ).	./cache/cord-004400-li1sc47z.txt	./txt/cord-004400-li1sc47z.txt