id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-005397-i7x50taa	Wang, Wen-juan	Preparation and identification of anti-rabies virus monoclonal antibodies	2012-06-09		.txt	text/plain	1426	82	46	To provide a foundation for the development of rapid and specific methods for the diagnosis of rabies virus infection, anti-rabies virus monoclonal antibodies were prepared and rabies virus nucleoprotein and human rabies virus vaccine strain (PV strain) were used as immunogens to immunize 6–8 week old female BALB/c mice. Spleen cells and SP2/0 myeloma cells were fused according to conventional methods: the monoclonal cell strains obtained were selected using the indirect immunofluorescence test; this was followed by preparation of monoclonal antibody ascitic fluid; and finally, systematic identification of subclass, specificity and sensitivity was carried out. Two high potency and specific monoclonal antibodies against rabies virus were obtained and named 3B12 and 4A12, with ascitic fluid titers of 1:8000 and 1:10000, respectively. Thus, the preparation of potent and specific monoclonal antibodies against rabies virus will contribute significantly to development of techniques for laboratory diagnosis of rabies.	./cache/cord-005397-i7x50taa.txt	./txt/cord-005397-i7x50taa.txt