id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-008407-jbp8bxjz	Derdeyn, Cynthia A.	Characterization of defective-interfering RNAs of rubella virusgenerated during serial undiluted passage	1995-01-10		.txt	text/plain	6710	298	56	During serial undiluted passage of rubella virus (RUB) in Vero cells, two species of defective-interfering (DI) RNAs of approximately 7000 and 800 nucleotides (nts) in length were generated (Frey, T. Finally, to determine whether the majority of DI RNAs present in P12 RNA contained a deletion in the SP-ORF, an oligonucleotide probe which is complementary to a sequence located within the E1 protein coding region (nts 8472 to 8488, eligonucleotide 83, Fig. 3E ) that was deleted in all three cDNA clones was hybridized to P12 RNA. Analysis of the 5' terminus of DI RNA generated during serial passage As shown in Figure 3A , oligonucleotide probes complementary to the exact 5' terminal sequences of the RUB genome hybridized to the large DI RNAs. To confirm that these DI RNAs contained the authentic 5' end of the genome, primer extension was performed on intraceilular RNA from standard RUB-infected cells and P12 RNA.	./cache/cord-008407-jbp8bxjz.txt	./txt/cord-008407-jbp8bxjz.txt