id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-253351-b36g09r0	Luo, Zongli	Roles in Cell-to-Cell Fusion of Two Conserved Hydrophobic Regions in the Murine Coronavirus Spike Protein	1998-05-10		.txt	text/plain	6840	356	57	Similar substitutions within PEP2 result in a fusion-negative phenotype; however, these mutant S proteins also exhibit defects in protein processing and surface expression which likely explain the loss of the ability to induce fusion. To determine the importance of the asymmetric distribution of hydrophobicity in the induction of cell-to-cell fusion, as predicted by the ability to model this peptide as a sided ␣ helix ( Fig. 2A) , nonconservative and conservative amino acid substitutions were introduced in PEP1 to target representative bulky hydrophobic residues (F977K, F977L, L981K, L981I) and polar or charged residues (S975D, N978D, N978L, D986V-D989V). All mutant PEP1 S proteins were assayed for fusion using both in situ and quantitative fusion assays, performed as shown in Fig. 3 , using the vTF7-3-infected and wild-type S gene-transfected cells as positive controls and vTF7-3-infected and mock-transfected cells as negative controls.	./cache/cord-253351-b36g09r0.txt	./txt/cord-253351-b36g09r0.txt