id author title date pages extension mime words sentences flesch summary cache txt cord-256703-eaj63c2k Matsuoka, Yumiko Intracellular accumulation of punta toro virus glycoproteins expressed from cloned cDNA 1988-11-30 .txt text/plain 4311 224 56 On the basis of genetic recombination and sequence analysis, it has been concluded that the S RNA encodes the nucleocapsid protein N and a nonstructural protein NS,; the M RNA encodes two glycoproteins Gl and G2 and in some genera a nonstructural protein, NS,; and the L RNA probably contains the information for the viral transcriptase (Bishop et al., 1982; Bouloy et a/., 1984; Cabradilla et al., 1983; Collett et a/., 1985; Eshita and Bishop, 1984; Fuller et al., 1983; lhara et a/., 1985; Lees et al., 1986; Ronnholm and Pettersson, 1987; Schmaljohn et a/., 1987 , 1982, 1984) . To compare the levels of PlV glycoprotein synthesis, monolayers of CV-1 cells were infected with wildtype vaccinia virus, PTV, or the vaccinia recombinants described above which contain the PTV glycoprotein gene with the different sequences in the translation initiation region. We have constructed vaccinia virus recombinants containing a partial cDNA clone of the M genome segment of PTV, which encodes the Gl and G2 glycoproteins. ./cache/cord-256703-eaj63c2k.txt ./txt/cord-256703-eaj63c2k.txt