id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-259095-mfptcw8t	Lu, Yiqi	Determinants of Mouse Hepatitis Virus 3C-like Proteinase Activity	1997-04-14		.txt	text/plain	4488	270	66	Numbering of amino acid residues within MHV 3CLpro is based The linear schematic of the MHV genome shows the organization of Ser 1 (Lu et al., 1995) Site-directed mutagenesis of pGpro with bovine chymotrypsin (Gorbalenya and Koonin, 1993) , 3Cpro of human rhinovirus 14 (Matthews et al., 1994) , and hepatitis A virus Asp53 and Asp65 were mutagenized by the Chame-3Cpro (Allaire et al., 1994) . The comparison of the were checked for residual expression and processing from the pGpro construct by the addition of [ 35 S]-four coronavirus 3CLpro sequences revealed two potential cleavage sites present only in MHV, QS 3554-5 , and methionine to an aliquot of the unlabeled reaction mixture after treatment with RNase and cycloheximide and QG 3607-8 . Mutation of Asp65 to Pro or Ala (D65P and D65A) MHV-A59, IBV, HCV-229E, and TGEV revealed no completely conserved Asp or Glu residues at positions analo-resulted in a proteinase with activity comparable to that expressed from wild-type pGpro (Fig. 3B, lanes 3 and 4) .	./cache/cord-259095-mfptcw8t.txt	./txt/cord-259095-mfptcw8t.txt